CN108353892A - The production method of rice plant sample - Google Patents
The production method of rice plant sample Download PDFInfo
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- CN108353892A CN108353892A CN201810012043.XA CN201810012043A CN108353892A CN 108353892 A CN108353892 A CN 108353892A CN 201810012043 A CN201810012043 A CN 201810012043A CN 108353892 A CN108353892 A CN 108353892A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
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- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to experimental technique fields, and specifically a kind of production method of rice plant sample, step include：Sampling with cleaning → guarantor it is green → plasticizing → embedding；The rice sample that this method is produced, green using completely new method guarantor, color is close under natural conditions with field, holding time is long, is less prone to the phenomenon gone mouldy and rotted, and the sample produced has stereoscopic effect, ornamental and highly practical, the authenticity of the rice retained to greatest extent.
Description
Technical field
The present invention relates to experimental technique field, a kind of specifically production method of rice plant sample.
Background technology
For plant specimen, when traditional squeeze preparation or humid preparation have been subjected to, disadvantage is apparent, is easy to rot,
Jaundice chlorosis is gone mouldy, and ornamental value is poor, it is difficult to be preserved for a long time.And plant specimen is made capable of effectively solving for amber form
Certainly these problems, and more beautiful exquisiteness.
Because different plants has the characteristics that different, requirement during preparation of specimen is also different, is mainly reflected in
Its blade and the moisture content of stalk, color, size, structure, flower, fruit etc. or even different growthdevelopmental stages its require also not
Together.The prior art mostly uses simply protect green processing after, compacting is then dried, be easy fade, make moist and go mouldy.
Invention content
The purpose of the present invention is existing in view of the above technology, a kind of production method of rice plant sample is provided.
To achieve the above object, the technical solution adopted by the present invention is：A kind of production method of rice plant sample, including
Following steps：
(1) sampling and cleaning：When temperature is higher, selection morphosis is complete, color is normal, without apparent disease and defect
Plant, whole strain maintain root to carry out single plant cleaning in water after taking out, and the plant after cleaning is primary with distilled water flushing；
(2) it protects green：Specimen material, which is put into, after cleaning protects in green solution, with sealing after being totally submerged, is during which seen every 3 days
Color change is examined, is taken out after 7-9 days and prepares plasticizing；
(3) it is plasticized：Protect it is green after specimen material with tap water rinse 2-3 time, clean after it is primary with distilled water flushing, flushing
Each concentration of polyethylene glycol that specimen material uses volumetric concentration to be 25%, 50%, 75% successively afterwards is impregnated 1 day, finally poly- with 100%
Ethylene glycol takes out after impregnating half a day, hangs 2-3 days after taking-up, is then placed in the baking oven of blotting paper in bottom, 40-45 DEG C of condition
Lower drying 5 days, being sealed after taking-up prevents from getting damp again；
(4) it embeds：The openable wooden box mold of surrounding is prepared, lastblock plastic film, wooden box surrounding are padded in wooden box bottom
It is fixed with adhesive tape, then in wooden box bottom surface and the upper pp plates of pad, prepares AB glue 320g, pouring into thickness on bottom plate is no more than
The AB glue of 0.8cm carries out second of glue after hardening, thickness is no more than 1.5cm, when glue thickness reaches required thickness half,
Portion is put into sample and shaping in a mold after equal glue hardening, AB glue and fixation is then uniformly spread on sample, after AB glue hardening
It recycles remaining AB glue to fill the other half, is demoulded after waiting for AB glue fully hardened；
(5) it polishes：Until the place sand paper polishing smoothly for occurring sharp water chestnut on sample edge；
(6) it is sealed：It is covered with the cloche of suitable size, and places drier in cloche, in dry after sealing
It is preserved in the environment in dry cool place.
Further, in step (2), guarantor's green solution is by 50% alcohol 850mL, copper acetate 25g, molecular weight 600
Polyethylene glycol 120mL, glacial acetic acid 60mL be formulated.
Further, in step (4), the weight proportion of A glue and B glue is 3 in the AB glue:1, towards one when mixing AB glue
Direction is stirred.
In practical preparation of specimen, the length of side of mold can be divided into：
Unilateral length≤the 10cm's of 0cm ＜, with silica gel mould or small-sized high plastotype box thoroughly.
Unilateral length≤the 25cm's of 10cm ＜, if any special size requirement, it is contemplated that fixed using pp materials in plastic mould factory
Do disposable tool；
Unilateral long ＞ 25cm's, using from molding jig, asks professional carpenter to carry out the openable wooden box in four sides by size, first exist
The good a little bit thicker plastic film of pad inside wooden box, then the pp plates on wooden box bottom surface and surrounding pad, especially require bottom surface
The smooth no marking of pp plates and smooth, surrounding pp plates can not be bent, and then close wooden box, and fixation is tied up with adhesive tape.This hair
The mold of bright mould therefor length of side ＞ 25cm.
In the present invention, for large-scale plant sample, it is also necessary to carry out shaping step, overall effect, which will reach, seems beautiful
Exquisiteness is seen, blade stalk can be upright, little with field shape difference.If stalk fractures, it can be cut off, by spare material
Material smears AB glue, is inserted into rice plant, does not affect the appearance.Curling for blade can use heater of hair straightener, be pressed
It is flat.Some places slightly damaged can be pinioned with transparent fish silk thread.In order to ensure that the spike of rice grain in maturity period does not fall, use
Syringe puts on syringe needle, cannot have glue to ooze as long as being applied to along the viscous a small amount of AB glue of grain ear branch stalk gently
It is existing.
The method have the benefit that：The rice sample that this method is produced protects green, color using completely new method
Close under natural conditions with field, the holding time is long, is less prone to the phenomenon gone mouldy and rotted, and the sample produced has solid
Effect watches and highly practical, the authenticity of the rice retained to greatest extent.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention without having to pay creative labor, may be used also for those of ordinary skill in the art
With obtain other attached drawings according to these attached drawings.
Fig. 1 is a kind of design sketch of sample that the present invention makes；
Fig. 2 is sample another design sketch that the present invention makes.
Specific implementation mode
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained without creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
(1) sampling and cleaning：When temperature is higher, selection morphosis is complete, color is normal, without apparent disease and defect
Plant, whole strain maintain root to carry out single plant cleaning in water after taking out, and the plant after cleaning is primary with distilled water flushing；
(2) it protects green：Specimen material, which is put into, after cleaning protects in green solution, with sealing after being totally submerged, is during which seen every 3 days
Color change is examined, is taken out after 7-9 days and prepares plasticizing；
(3) it is plasticized：Protect it is green after specimen material with tap water rinse 2-3 time, clean after it is primary with distilled water flushing, flushing
Each concentration of polyethylene glycol that specimen material uses volumetric concentration to be 25%, 50%, 75% successively afterwards is impregnated 1 day, finally poly- with 100%
Ethylene glycol takes out after impregnating half a day, hangs 2-3 days after taking-up, is then placed in the baking oven of blotting paper in bottom, 40-45 DEG C of condition
Lower drying 5 days, being sealed after taking-up prevents from getting damp again；
(4) it embeds：The openable wooden box mold of surrounding is prepared, lastblock plastic film, wooden box surrounding are padded in wooden box bottom
It is fixed with adhesive tape, then in wooden box bottom surface and the upper pp plates of pad, prepares AB glue 320g, pouring into thickness on bottom plate is no more than
The AB glue of 0.8cm carries out second of glue after hardening, thickness is no more than 1.5cm, when glue thickness reaches required thickness half,
Portion is put into sample and shaping in a mold after equal glue hardening, AB glue and fixation is then uniformly spread on sample, after AB glue hardening
It recycles remaining AB glue to fill the other half, is demoulded after waiting for AB glue fully hardened；
(5) it polishes：Until occurring the place of sharp water chestnut on sample edge with sand paper polishing smoothly, preparation of specimen is complete
Finish, it is as shown in Figure 1 or 2 to make later sample.
Wherein, green solution is protected by 50% alcohol 850mL, copper acetate 25g, the polyethylene glycol 120mL that molecular weight is 600, ice
Acetic acid 60mL is formulated.
Wherein, the weight proportion of A glue and B glue is 3 in AB glue:1, it is stirred in one direction when mixing AB glue.
Embodiment 2
1, sampling and cleaning
The quality of material is to make the first step of sample, principle be choose morphosis is complete, representative strong, color just
Often, without apparent disease and defect, the integrality of its morphosis should be kept to greatest extent in sampling, avoided damage to.
The sampling and cleaning of 1.1 rice plants or portion of tissue
When field samples, choose morphosis it is complete, it is representative it is strong, color is normal, without apparent disease and defect.By
General temperature is higher when being easy dehydration curling in rice leaf, and sample, and existing enchashment is used, should whole pocket even soil in sampling
It takes out, ensures that plant is fresh and alive, preferably cleaned in larger basin when cleaning soil, the moment keeps root in water, can incite somebody to action
One pocket is broken into two with one's hands, single plant cleaning, soil wash clean, it should be specifically noted that avoiding mechanical damage in cleaning process.It will finally clean
Sample distilled water flushing one time.
2, green and plasticizing is protected
2.1 guarantors are green
Due to the soft easily volume of rice leaf, discomfort shares that the method that copper acetate boils is green to protect, and blade is easy to become more
It is soft, it is difficult to it is upright, and also it is also highly difficult to handle whole pocket plant concrete operations.The method impregnated with copper sulphate is also undesirable.Therefore it adopts
With new protection method for green, it is formulated as follows：
Prepare big container, what guarantee rice plant can unfold is put into wherein, mechanical damage is avoided, then needed for
Liquor capacity is matched in proportion, after mixing, makes acetic acid copper dissolution as possible, and the specimen material after cleaning then is put into solution
In, submergence, record impregnates the date.It since acetic acid and alcoholic solution volatilize, need to be covered, be sealed with plastic film.After 3 days, every
Open within one day observation color change, color should be a process of green → yellow → green, can be taken out after about seven days, or
Greening.It is plasticized after taking-up.
2.2 plasticizing
It should be rinsed 2-3 times, be cleaned with tap water before plasticizing, it is then primary with distillation washing.Polyethylene glycol has good
Water solubility, can be slowly substitution blade in moisture, achieve the effect that one plasticizing, the rice leaf after plasticizing is straight and upright, machine
Tool intensity increases, and colour brightness increases, more added with texture.Polyethylene glycol is made into 4 gradients with distilled water, 25%, 50%,
75%, 100%.First three gradient, each gradient are steeped one day, and 100% polyethylene glycol steeps oneself-meeting and blade is made to fade, and green shoals,
Blade becomes saturating, therefore only needs to steep half a day, must take out.
Polyethylene glycol due to 100% is more sticky and is difficult to volatilize, and after taking-up, is pinioned and is drained 2-3 days with rope,
Extra polyethylene glycol leaches.It is subsequently placed into big baking oven and dries, water suction is padded in oven temperature control below 40-45 degree, baking oven
Paper until feeling not extra polyethylene glycol (about 5 days) with hand, and feels drying.After being taken out in baking oven, plastics are used
Film encases, and prevents from getting damp again.It prepares for next step embedding.
3, mold and embedding
The preparation of 3.1 molds
There are many molds, and basic demand is both materials and asphalt mixtures modified by epoxy resin based on pp materials or silica gel material
Fat (AB glue) does not glue.
As reference with the mold of quadrangle：
0cm≤unilateral length≤10cm's, with silica gel mould, (due to AB glue condensation heat, silica gel mould is easy greatly very much to become
Shape) or small-sized high plastotype box thoroughly.
10cm≤unilateral length≤25cm's, if any special size requirement, it is contemplated that fixed using pp materials in plastic mould factory
Do disposable tool
Unilateral length >=25cm's, using from molding jig.Please professional carpenter carry out the openable wooden box in 4 faces by size, first exist
The good a little bit thicker plastic film (preventing AB glue from draining on wooden box, conveniently stripped) of pad inside wooden box, then wooden box bottom surface with
And pp plates on surrounding pad, the smooth no marking of pp plates of bottom surface and smooth is especially required, surrounding pp plates can not be bent, and then be closed
Wooden box ties up fixation with adhesive tape.
3.2 match glue
AB glue type is various, need to do sample with high saturating and inhibited from yellowing AB glue.Match by weight, A:B=3:1.AB glue
Mixing vessel uses the disposable transparent lunch box bowl of 480ml.With A glue 240g, B glue 80g, total 320g, as standard.A glue B glue is mixed
It with disposable spoon after conjunction, is stirred in one direction, reduces bubble and generate, until transparent, no silk is then allowed to stand and disappears
Bubble.
3.3 samples are shaped and embedding (with environment temperature, 25 degree are reference)
Since AB glue cannot be mixed disposably too much, in condensation process can heat release, after temperature build, be easy to cause implode,
And if the pp plates of mold bottom surface are larger, and temperature is too high also to be deformed, therefore can only embed down glue by several times, and bottom surface is for the first time
Glue, should be less, and thickness does not exceed 0.8cm, behind the single of falling glue by several times thickness no more than 1.5cm, fall every time
After glue should wait a preceding glue to harden, can just it fall down primary.The sample buried should pour into required thickness in the centre of sample
When the half of degree, after AB glue hardening, it is put into sample, then AB glue is uniformly spread in shaping on sample, fixed, and in this way
The bubble on sample is can effectively prevent to generate and sample floating.Etc. after AB glues have done, sample just secures, and then uses glue handle
Remaining half fills.
Rice large size plant sample embeds, it is impossible to all embeds into AB glue, it can only be with above 10cm in root or so
Embedding enables rice plant to erect and comes.According to rice tillering spy and Characters of Plant Type, one plant of strain rice is stitched together, upper
Middle and lower part is tied up with rope come lower part will tighten, and be conveniently operated.After the glue that fallen in mold carries out bottom, rice plant is tied up in wood
On frame, allow its perpendicular to mold center, then in layer fall glue.Before last layer of glue, lower part rope is taken off,
At rice plant lower part is from root about 25cm, with syringe by AB glue injection rice plant stalk gap, it can inject several times,
Glue is not got outside stalk, allow between stalk and cement.Finally just remaining glue is filled.Then with special cloche,
It covers, drier is placed in the inside, and seals.
The seed specimens of square-outside and round-inside make, and transparent culture ware lid or bottom can be used, culture dish is paved with by seed
It is flat, it then falls glue inside, fills as possible, and observe whether bottom has bubble.At the same time bottom can be carried out in a mold.Deng all
After hardening, in embedding, first one bowl of glue, culture dish is put down from one side slowly have prevented bubble (just as lid coverslip one
Sample).Then remaining glue is filled in layer again.
3.4 demoulding
Etc. AB glues it is dry after, touched with hand and feel hardened, then etc. 2 days, wait for that AB glue parches, can just demould.Silica gel mould and
Disposable tool demoulding is easier to, and can directly be dismantled.From molding jig, first wooden box four sides is opened, plastic film is taken out.
4, polishing and shaping
No matter the sample which kind of mold is made just has slightly sharp place since surface can be tensioned, need to beat
Mill can first be polished with thick sand paper, then be polished with thin sand paper, it is known that not until oarsman.It is had from molding jig relatively rough
Place, be difficult to be polished flat with sand paper, cutting machine can be touched with hand, adapted cotton flywheel is polished.Such as cutting machine is touched with hand
Polishing plant sample, should wrap rice plant with plastic film, prevents airborne dust, and should all wear yarn hand in operating process
Set.
Shaping is primarily directed to large-scale plant sample, and overall effect, which will reach, seems beautiful exquisite, blade stalk energy
It is enough upright, it is little with field shape difference.It if stalk fractures, can be cut off, by spare material, AB glue be smeared, by it
It is inserted into rice plant, does not affect the appearance.Curling for blade can use heater of hair straightener, be pressed.What some were slightly damaged
Place can be pinioned with transparent fish silk thread.In order to ensure that the spike of rice grain in maturity period does not fall, with syringe, syringe needle, edge are put on
The viscous a small amount of AB glue of grain ear branch stalk gently, as long as being applied to, there cannot be glue to ooze existing.
Claims (3)
1. the production method of rice plant sample, which is characterized in that include the following steps：
(1) sampling and cleaning：When temperature is higher, selection morphosis is complete, color is normal, is planted without apparent disease and defect
Strain, whole strain maintain root to carry out single plant cleaning in water after taking out, and the plant after cleaning is primary with distilled water flushing；
(2) it protects green：Specimen material, which is put into, after cleaning protects in green solution, with sealing after being totally submerged, during which observes face every 3 days
Color change takes out after 7-9 days and prepares plasticizing；
(3) it is plasticized：Protect it is green after specimen material rinsed 2-3 time with tap water, it is clean after, flushing after sample primary with distilled water flushing
Each concentration of polyethylene glycol that product material uses volumetric concentration to be 25%, 50%, 75% successively is impregnated 1 day, finally with 100% poly- second two
Alcohol takes out after impregnating 12h, is hung 2-3 days after taking-up, is then placed in the baking oven of blotting paper in bottom, is dried under the conditions of 40-45 DEG C
5 days dry, being sealed after taking-up prevents from getting damp again；
(4) it embeds：The openable wooden box mold of surrounding is prepared, pads lastblock plastic film in wooden box bottom, wooden box surrounding is with thoroughly
Gelatin band is fixed, and then in wooden box bottom surface and the upper pp plates of pad, prepares AB glue 320g, pouring into thickness on bottom plate is no more than
The AB glue of 0.8cm carries out second of glue after hardening, thickness is no more than 1.5cm, when glue thickness reaches required thickness half,
Portion is put into sample and shaping in a mold after equal glue hardening, AB glue and fixation is then uniformly spread on sample, after AB glue hardening
It recycles remaining AB glue to fill the other half, is demoulded after waiting for AB glue fully hardened；
(5) it polishes：Until the place sand paper polishing smoothly for occurring sharp water chestnut on sample edge；
(6) it is sealed：It is covered with the cloche of suitable size, and places drier in cloche, in dry cloudy after sealing
It is preserved in cool environment.
2. the production method of rice plant sample according to claim 1, which is characterized in that in step (2), the guarantor is green molten
Liquid is formulated by 50% alcohol 850mL, copper acetate 25g, polyethylene glycol 120mL, the glacial acetic acid 60mL that molecular weight is 600.
3. the production method of rice plant sample according to claim 1, which is characterized in that in step (4), in the AB glue
The weight proportion of A glue and B glue is 3:1, it is stirred in one direction when mixing AB glue.
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| CN201810012043.XA CN108353892A (en) | 2018-01-05 | 2018-01-05 | The production method of rice plant sample |
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| CN110447423A (en) * | 2019-08-26 | 2019-11-15 | 河北省农林科学院旱作农业研究所 | A kind of millet or wheat root preparation of specimen device and method |
| CN114982745A (en) * | 2022-04-29 | 2022-09-02 | 濮阳市食品药品检验检测中心 | Method for preparing plant-soaked specimen |
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