CN106359369A - Making method for liquid-preserved specimen of wheat plant - Google Patents
Making method for liquid-preserved specimen of wheat plant Download PDFInfo
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- CN106359369A CN106359369A CN201610639019.XA CN201610639019A CN106359369A CN 106359369 A CN106359369 A CN 106359369A CN 201610639019 A CN201610639019 A CN 201610639019A CN 106359369 A CN106359369 A CN 106359369A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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Abstract
The invention provides a making method for a liquid-preserved specimen of a wheat plant. The making method comprises the following steps: digging of a wheat plant from field soil; flushing of roots; removal of foreign materials in the roots; chemical treatment of the whole wheat plant; removal of a treatment fluid; soaking with formaldehyde liquid; shaping of a specimen; and hermetic preservation of the specimen in a transparent acrylic synthetic-glass container. The method is applicable to making of liquid-preserved specimens of tissue culture seedlings, whole plants, underground roots or the like of triticeae crops (including transgenic materials) under all soil cultivation conditions and non-soil cultivation conditions.
Description
Technical field
The present invention relates to a kind of plant specimen manufacture method, more particularly, to a kind of making of wheat plant liquid humid preparation
Method.
Background technology
Semen Tritici aestivi is one of important cereal crops of China, and its yield level is most important to guarantee national food security.Little
Wheat plant is mainly made up of aerial partss stem and leaf fringe and under ground portion root system.Aerial partss are photosynthetic important places.Ground
Lower part is the important nutrition organs of Semen Tritici aestivi, and the root quantity of Semen Tritici aestivi, weight, thickness, length etc. directly affect wheat plant pair
Moisture and the absorption of nutrition, and the lodging tolerance of Semen Tritici aestivi individual plant and its colony.Semen Tritici aestivi relies on its complicated huge
System absorbs water and nutrition, to meet the metabolic processes of Semen Tritici aestivi from soil.Strong root system is to obtain improving yield of wheat
Important substance basis.The character such as the blade of Semen Tritici aestivi aerial partss, stalk, fringe portion, seed are that breeder observes most agronomy
Shape, but very important root system is partially due to be longer than deep subsoil it is difficult to directly be observed to it, and then affect to itself and ground
The analysis of upper Some Agronomic Traits dependency.Therefore, make the complete specimen of wheat plant, can intuitively observe the ground of Semen Tritici aestivi
The upgrowth situation of upper and underground two parts organ, and then it is compared analysis with other economical characters.
The method making plant specimen at present, primarily focuses on the preparation of specimen of aerial partss rhizome foliage organ, because ground
Upper part collection is easier.Root system of plant specimen is different from general plant specimen, and collection difficulty is big, bright to vegetation deterioration
Aobvious, thus make difficult.Semen Tritici aestivi is fibrous root, and the collection of fibrous root is more difficult.General wheat plant specimen only gathers close
The sub-fraction root system on ground, the root system of Semen Tritici aestivi is very flourishing in fact, is limited to current acquisition method and makes specimen method, little
The complete root system preparation of specimen of wheat is particularly difficult.Current wheat plant specimen mostly is the dry preserved specimen after Semen Tritici aestivi maturation, and
Mostly directly extract from soil, only leave less root system, most of root system is pulled off it is impossible to really reflect wheat root
Upgrowth situation.Most of Semen Tritici aestivi liquid humid preparations at present, are only to choose fringe portion and plant top 2-3 section leaf production,
The whole strain specimen of Semen Tritici aestivi, this be due to lack on market volume and highly on be suitable for wheat strain preparation of specimen glass
Glass container height highest on container, such as market is less than 50cm, and wheat plant only aerial partss are generally just in 80cm,
The plant height of some Wheat Germplasm Resources is in more than 1m, so the glass container on market cannot meet wheat strain specimen
Make.There is no the liquid seeing wheat root humid preparation itself and the wheat strain including aerial partss organ at present
Humid preparation.
Content of the invention
For solving the deficiency in prior art, the invention provides one kind takes wheat plant, root system from field soil
Flushing, removal root system debris, whole strain chemical treatment, removal treatment fluid, formaldehyde liquid immersion, specimen shaping, transparent acrylic have
The manufacture method of the wheat plant liquid humid preparation of machine glass container sealing preserve.The present invention is adapted to wheat crops and (includes
Transgenic line) under all soil cultivation condition and under non-soil cultivation condition as tissue culturing seedling and whole plant or only
The making of the root system partially liq humid preparation of underground.
The present invention is achieved by the following technical solutions:
1) candidate plant is selected
In the field plots of plantation Semen Tritici aestivi, choose eugonic plant as the candidate plant making specimen, and listing mark,
Then other unwanted plant shoots are pulled out；Choose more than 3 candidate plant, space between plants will be remained above or be equal to 70
cm；Or in wheat cultivation, wheat cultivation spacing is remained above or is equal to 70 cm；
2) earthwork is selected
With Semen Tritici aestivi candidate plant as the center of circle, draw circle according to 30cm radius, vertically insert a diameter of 0.5cm, length to soil layer in circumference
Reinforcing bar for 1m, bar spacing 10cm about；
3) the taking of wheat plant
Around the reinforcing bar periphery of insertion soil layer, using shoveling instrument, take internal diameter be 30cm, the cylindrical soil of a height of more than 1m
Side；Meanwhile, in cylindrical earthwork periphery opposite sides, 2 relative, more than internal diameter 30cm, the working linings of deep about 40cm are left
Face, is beneficial to subsequent job operation；
4) rush soil and wash root
Using water injector, gradual flushing is carried out to the cylindrical earthwork；When rinsing, current can not be too strong, in order to avoid causing big soil
Dropping of block, the root system of attachment is broken；The soil layer of cylindrical earthwork 30-40cm is relatively soft, should slowly rinse, the knot of flushing
Really, should be that soil layer above the cylindrical earthwork gradually tails off, soil layer below is slightly many, form an equilateral triangle structure, otherwise
Mound easily collapses；Cylindrical earthwork bottom solum relative rigid, with the water soaked overnight that about 50cm is deep, simultaneously with moistening gauze
By root system parcel exposed for top, in order to avoid root system is dried and damages；Cylindrical earthwork bottom soil block, is ensureing that root system is injury-free
When, can suitably increase flow intensity；Unnecessary current and mud, can be taken out by reserved working interface；
5) carry the taking-up of wheat root mound
Wheat plant root system water rush to mound there are about 10-15kg weight when, by hole in muddy water all take out, and to hole in fill
Dry ground extremely can bear certain human body weight；In hole, using instruments such as scuppits from mound bottom, slowly excavated,
Mound bottom is made to depart from soil eventually；Mound with root system is gently put in plastic box, and ajusts, then water is filled, leaching
Steep and fade to muddy to soil block；
6) wash root
Gently rinse root system using tap water clear to water；When rinsing, to little clod, ensureing the injury-free premise of root system
Under, gently pulverized with handss in water；
7) debris on root are removed
The wheat plant of acquisition is placed in the plastic tub containing clear water, using small-sized tweezers or free-hand will be attached on root system
The debris such as rotten weeds remove totally, and gently ajust root system for growth attitude, are that the process of specimen subsequent chemistry is prepared；
8) plant Schweinfurt green heat treatment
When Schweinfurt green treatment fluid is heated to 85 DEG C, pour 120 cm length into, 25 cm are wide, 25 cm the are high transparent organic glass of acrylic
In glass groove, it is then placed in washing clean fresh wheat plant and carries out chemical treatment；During chemical treatment, by heater to place
Reason liquid is constantly heated, and so that the liquid temperature is controlled at 80 DEG C about, and material process needs 15 min, wheat plant by green by
Gradual change Huang, browning, last be changed into taking out material during green again, and with clear water, the material processing is rinsed 2-3 time；Processing procedure
To carry out in ventilation, and often to stir wheat plant, so that its uniform coloring；
9.) the fixing shaping of specimen
Prepare 5% formalin, and inject in the cylindrical transparent acrylic preparation jar of diameter 20 cm, high 120 cm；Using 2
Symmetrical setline or white Nylon line tie down the upper end of plant root, then from the beginning of plant root lower end, specimen are blown slowly
Put into and fill in the preparation jar preserving liquid, make preservation immersion not have specimen；Adjust plant attitude so as to root system is in plumbness,
Then specimen is fixed；Preparation jar lid is coated vaseline, then covers preparation jar mouth and carry out pressurizing window, be placed in cool place
Place is long-term to be preserved；During preserving, if preserving liquid turbid phenomenon, the formaldehyde that should more renew at once preserves liquid.
1st) in step, described candidate plant select, period be preferably Semen Tritici aestivi period of seedling establishment before.
2nd) in step, the described earthwork select, period is preferably wheat during jointing stage.
8th) in step, described plant Schweinfurt green heat treatment, the compound method of described Schweinfurt green treatment fluid is: according to 180 g
Schweinfurt green, 500 ml glacial acetic acid, water 500 ml proportions become saturated solution, then this saturated solution is diluted 5 times.
The invention has the beneficial effects as follows:
1, the invention provides to the wheat root method of sampling, the method is little to wheat root damage, is conducive to preserving root system
Natural growth conditions.
2nd, the present invention is conducive to wheat root being preserved for a long time and studying.
3rd, the present invention can according to the differing heights of Semen Tritici aestivi and size, adjust preserve specimen transparent organic glass container and
The lucite groove of sample disposal, is easy to make the whole wheat plant mark of differing heights and size including complete root system
This.
Brief description
The taking of Fig. 1 wheat plant.
Fig. 2 punching is native and washes root.
The wheat plant root system that Fig. 3 flushing takes.
Fig. 4 removes the debris on wheat root and root system is arranged.
Fig. 5 carries out Schweinfurt green heat treatment to wheat plant.
The wheat lines that Fig. 6 clear water rinsing was processed.
Fig. 7 prepares formalin and injects in cylindrical transparent acrylic preparation jar.
The wheat plant liquid humid preparation that Fig. 8 completes.
Specific embodiment
With reference to embodiment the present invention is done with further detailed description:
The making of embodiment 1 Semen Tritici aestivi specimen
In Shandong Academy of Agricultural Sciences Jinan experimental farm, carry out wheat planting and sampling, make specimen.
1. plant is selected
In the field plots of plantation Semen Tritici aestivi, before Semen Tritici aestivi period of seedling establishment, choose eugonic plant as the candidate making specimen
Plant, and listing mark, then pull out other unwanted plant shoots.Typically choose 3 candidate plant, plant has an area of spacing
Keep 70 cm；Or in wheat cultivation, wheat cultivation spacing keeps 70 cm.
2. selecting cubic metre of earth
In wheat during jointing stage, around the Semen Tritici aestivi candidate plant selected, diameter 0.5cm is vertically inserted to soil layer for radius with 30cm
The long reinforcing bar of 1m, bar spacing 10cm about (Fig. 1).
3. the taking of wheat plant
Round the reinforcing bar periphery of insertion, using instruments such as spade, pick, spades, take internal diameter 30cm, the soil of deep more than 1m
Layer.Meanwhile, outside original internal diameter 30cm, leave 2 relative, more than internal diameter 30cm, the working surfaces of deep about 40cm, be beneficial to
To the digging out of bottom solum, subsequently unnecessary muddy water take and whole plant and root system taking-up (Fig. 1).
4. rush soil and wash root
The agricultural water injector being equipped with using driven high voltage hydraulic giant or tractor or piston type children toy water gun, to selected plant
Soil layer carry out gradual flushing.Make sure to keep in mind, when rinsing, current can not be too strong, in order to avoid causing dropping of large clod, by attachment
Root system is broken.The soil layer of 30-40cm is relatively soft, should slowly rinse, the result of flushing, should be that soil layer above tails off, below
Soil layer slightly many, formed an equilateral triangle structure, otherwise mound easily collapse.Bottom solum relative rigid, with pouring about
Root system exposed for mound top is wrapped up with moistening gauze by the deep water soaked overnight of 50cm simultaneously, in order to avoid root system is dried and damages.
To bottom soil block, when ensureing that root system is injury-free, can suitably increase flow intensity.Unnecessary current and mud, Ke Yitong
Cross reserved working interface, repeatedly taken out (Fig. 2) by vessel such as beaker, spades.
5. carry the taking-up of wheat root mound
Whne wheat plant root system rush there are about 10-15kg weight to mound when, the muddy water in hole is all taken out, and dry to landfill in hole
Soil extremely can bear certain human body weight.In hole, using instruments such as scuppits from mound bottom, slowly excavated, finally
Mound is made to separate with bottom.Mound with root system is gently put in plastic box, and ajusts, then water is filled, be dipped to
Soil block fades to muddy.
6. wash root
After the clod in mound is dipped to mud, gently rinsed clear to water using tap water.When rinsing, to little clod,
Under the premise of ensureing that root system is injury-free, in water, gently pulverized (Fig. 3) with handss.
7. remove the debris on root
The wheat plant of acquisition is placed in the plastic tub containing clear water, using small-sized tweezers or free-hand will be attached on root system
The debris such as rotten weeds remove totally, and gently ajust root growth attitude, are that specimen staining prepares (Fig. 4).
8. plant Schweinfurt green heat treatment
First by 180 g Schweinfurt greens, 500 ml glacial acetic acid, 500 ml that add water stir into saturated solution, then this saturated solution is diluted 5 times
It is material process liquid.When treatment fluid is heated to 85 DEG C, pour 120 cm length into, 25 cm are wide, 25 cm are high acrylic transparent
In lucite groove, it is then placed in washing clean fresh wheat plant and carries out chemical treatment (Fig. 5).During chemical treatment, lead to
Cross heater treatment fluid is constantly heated, so that liquid temperature is controlled at 80 DEG C about, material process needs 15 min, treat that Semen Tritici aestivi is planted
Strain is gradually turned yellow from green, browning, last be changed into during green taking out material, and with clear water to the material rinsing 2-3 processing again
Secondary (Fig. 6).Note to carry out in ventilation in processing procedure, and often stir material, so that its uniform coloring.
9. the fixing shaping of specimen
Prepare 5% formalin, and inject in the cylindrical transparent acrylic preparation jar of diameter 20 cm, high 120 cm (Fig. 7).Profit
Tie down the upper end of plant root with 2 symmetrical setlines or white Nylon line, then from the beginning of plant root lower end, by specimen
Slowly put into and fill in the preparation jar preserving liquid, do not cross specimen.Adjustment plant attitude is so as to root system is in plumbness, so
Afterwards specimen is fixed.Preparation jar lid is coated vaseline, then covers preparation jar mouth and carry out pressurizing window, be placed in shady place
Long-term preservation (Fig. 8).During preserving, if preserving liquid turbid phenomenon, the formaldehyde that should more renew at once preserves liquid.
The above is only the preferred implementation of this patent it is noted that ordinary skill people for the art
For member, on the premise of without departing from the art of this patent principle, some improvement can also be made and replace, these improve and replace
Also should be regarded as the protection domain of this patent.
Claims (4)
1. a kind of manufacture method of wheat plant liquid humid preparation, comprises the following steps:
1) candidate plant is selected
In the field plots of plantation Semen Tritici aestivi, choose eugonic plant as the candidate plant making specimen, and listing mark,
Then other unwanted plant shoots are pulled out；Choose more than 3 candidate plant, space between plants will be remained above or be equal to 70
cm；Or in wheat cultivation, wheat cultivation spacing is remained above or is equal to 70 cm；
2) earthwork is selected
With Semen Tritici aestivi candidate plant as the center of circle, draw circle according to 30cm radius, vertically insert a diameter of 0.5cm, length to soil layer in circumference
Reinforcing bar for 1m, bar spacing 10cm about；
3) the taking of wheat plant
Around the reinforcing bar periphery of insertion soil layer, using shoveling instrument, take internal diameter be 30cm, the cylindrical soil of a height of more than 1m
Side；Meanwhile, in cylindrical earthwork periphery opposite sides, 2 relative, more than internal diameter 30cm, the working linings of deep about 40cm are left
Face, is beneficial to subsequent job operation；
4) rush soil and wash root
Using water injector, gradual flushing is carried out to the cylindrical earthwork；When rinsing, current can not be too strong, in order to avoid causing big soil
Dropping of block, the root system of attachment is broken；The soil layer of cylindrical earthwork 30-40cm is relatively soft, should slowly rinse, the knot of flushing
Really, should be that soil layer above the cylindrical earthwork gradually tails off, soil layer below is slightly many, form an equilateral triangle structure, otherwise
Mound easily collapses；Cylindrical earthwork bottom solum relative rigid, with the water soaked overnight that about 50cm is deep, simultaneously with moistening gauze
By root system parcel exposed for top, in order to avoid root system is dried and damages；Cylindrical earthwork bottom soil block, is ensureing that root system is injury-free
When, can suitably increase flow intensity；Unnecessary current and mud, can be taken out by reserved working interface；
5) carry the taking-up of wheat root mound
Wheat plant root system water rush to mound there are about 10-15kg weight when, by hole in muddy water all take out, and to hole in fill
Dry ground extremely can bear certain human body weight；In hole, using instruments such as scuppits from mound bottom, slowly excavated,
Mound bottom is made to depart from soil eventually；Mound with root system is gently put in plastic box, and ajusts, then water is filled, leaching
Steep and fade to muddy to soil block；
6) wash root
Gently rinse root system using tap water clear to water；When rinsing, to little clod, ensureing the injury-free premise of root system
Under, gently pulverized with handss in water；
7) debris on root are removed
The wheat plant of acquisition is placed in the plastic tub containing clear water, using small-sized tweezers or free-hand will be attached on root system
The debris such as rotten weeds remove totally, and gently ajust root system for growth attitude, are that the process of specimen subsequent chemistry is prepared；
8) plant Schweinfurt green heat treatment
When Schweinfurt green treatment fluid is heated to 85 DEG C, pour 120 cm length into, 25 cm are wide, 25 cm the are high transparent organic glass of acrylic
In glass groove, it is then placed in washing clean fresh wheat plant and carries out chemical treatment；During chemical treatment, by heater to place
Reason liquid is constantly heated, and so that liquid temperature is controlled at 80 DEG C about, material process needs 15 min about, when wheat plant is by green
Gradually flavescence, browning, last be changed into taking out material during green again, and with clear water, the material processing is rinsed 2-3 time；Processed
Journey will be carried out in ventilation, and often stirs wheat plant, so that its uniform coloring；
9.) the fixing shaping of specimen
Prepare 5% formalin, and inject in the cylindrical transparent acrylic preparation jar of diameter 20 cm, high 120 cm；Using 2
Symmetrical setline or white Nylon line tie down the upper end of plant root, then from the beginning of plant root lower end, specimen are blown slowly
Put into and fill in the preparation jar preserving liquid, make preservation immersion not have specimen；Adjust plant attitude so as to root system is in plumbness,
Then specimen is fixed；Preparation jar lid is coated vaseline, then covers preparation jar mouth and carry out pressurizing window, be placed in cool place
Place is long-term to be preserved；During preserving, if preserving liquid turbid phenomenon, the formaldehyde that should more renew at once preserves liquid.
2. the manufacture method of a kind of wheat plant liquid humid preparation according to claim 1, the wherein the 1st) in step, described
Candidate plant select, period be Semen Tritici aestivi period of seedling establishment before.
3. the manufacture method of a kind of wheat plant liquid humid preparation according to claim 2, the wherein the 2nd) in step, described
Cubic metre of earth select, period be wheat during jointing stage.
4. the manufacture method of a kind of wheat plant liquid humid preparation according to claim 3, the wherein the 8th) in step, described
Plant Schweinfurt green heat treatment, the compound method of described Schweinfurt green treatment fluid is: according to 180 g Schweinfurt greens, 500 ml glacial acetic acid, water
500 ml proportions become saturated solution, then this saturated solution is diluted 5 times.
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| CN201610639019.XA CN106359369B (en) | 2016-08-08 | 2016-08-08 | A kind of production method of wheat plant liquid humid preparation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108353892A (en) * | 2018-01-05 | 2018-08-03 | 湖南农业大学 | The production method of rice plant sample |
| CN110447423A (en) * | 2019-08-26 | 2019-11-15 | 河北省农林科学院旱作农业研究所 | A kind of millet or wheat root preparation of specimen device and method |
| CN113207873A (en) * | 2021-05-19 | 2021-08-06 | 石家庄市农林科学研究院 | Method for preparing primary color wheat soaked specimen |
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| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
| CN101401567A (en) * | 2008-03-19 | 2009-04-08 | 中国科学院新疆生态与地理研究所 | Eremophyte steeping specimen and method for making protective color |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
| CN103392694A (en) * | 2013-08-26 | 2013-11-20 | 中国医学科学院药用植物研究所海南分所 | Method for preparing tropical plant preserving humid preparation |
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| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
| CN101401567A (en) * | 2008-03-19 | 2009-04-08 | 中国科学院新疆生态与地理研究所 | Eremophyte steeping specimen and method for making protective color |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108353892A (en) * | 2018-01-05 | 2018-08-03 | 湖南农业大学 | The production method of rice plant sample |
| CN110447423A (en) * | 2019-08-26 | 2019-11-15 | 河北省农林科学院旱作农业研究所 | A kind of millet or wheat root preparation of specimen device and method |
| CN113207873A (en) * | 2021-05-19 | 2021-08-06 | 石家庄市农林科学研究院 | Method for preparing primary color wheat soaked specimen |
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| CN106359369B (en) | 2019-11-26 |
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