CN106359369B - A kind of production method of wheat plant liquid humid preparation - Google Patents
A kind of production method of wheat plant liquid humid preparation Download PDFInfo
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- CN106359369B CN106359369B CN201610639019.XA CN201610639019A CN106359369B CN 106359369 B CN106359369 B CN 106359369B CN 201610639019 A CN201610639019 A CN 201610639019A CN 106359369 B CN106359369 B CN 106359369B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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Abstract
The production method for the wheat plant liquid humid preparation that the present invention provides a kind of to take wheat plant from field soil, root system rinses, removal root system sundries, whole strain chemical treatment, removal treatment fluid, formaldehyde liquid immersion, sample shaping, transparent acrylic plexiglass box are sealed.The present invention is adapted to wheat crops (including transgenic line) under all soil cultivation conditions and under non-soil cultivation condition such as tissue culturing seedling and whole plant or the only production of the root system partially liq humid preparation of underground.
Description
Technical field
The present invention relates to a kind of plant specimen production method more particularly to a kind of production of wheat plant liquid humid preparation
Method.
Background technique
Wheat is one of the important cereal crops in China, and yield level is most important to guarantee national food security.It is small
Wheat plant is mainly made of aerial part cauline leaf fringe and under ground portion root system.Aerial part is photosynthetic important place.Ground
Lower part is the important nutrition organs of wheat, and root quantity, weight, thickness, length of wheat etc. directly affect wheat plant pair
The absorption and wheat single plant of moisture and nutrition and its lodging tolerance of group.Wheat relies on its complicated huge
System absorbs water from soil and nutrition, to meet the metabolic processes of wheat.Strong root system is to obtain improving yield of wheat
Important material base.The characters such as blade, stalk, fringe portion, the seed of wheat aerial part are that breeder observes most agronomy
Shape, but very important root system is partially due to be longer than deep subsoil, it is difficult to it is directly observed, and then is influenced to itself and ground
The analysis of upper Some Agronomic Traits correlation.Therefore, the complete specimen of wheat plant is made, the ground of wheat can be intuitively observed
Upper and underground two parts organ upgrowth situation, and then analysis is compared with other economical characters.
The method of production plant specimen at present, primarily focuses on the preparation of specimen of aerial part rhizome foliage organ, because of ground
Upper part acquisition is easier.Root system of plant sample is different from general plant specimen, and acquisition difficulty is big, bright to vegetation deterioration
It is aobvious, thus make difficult.Wheat is fibrous root, and the acquisition of fibrous root is more difficult.General wheat plant sample only acquires close
The sub-fraction root system on ground, the root system of wheat is very flourishing in fact, is limited to current acquisition method and production sample method, small
The complete root system preparation of specimen of wheat is particularly difficult.Current wheat plant sample is mostly the dry preserved specimen after wheat is mature, and
It is mostly directly extracted from soil, only there are less root system, most of root system is pulled off, and cannot really reflect wheat root
Upgrowth situation.Current most of wheat liquid humid preparations are only to choose fringe portion and plant top 2-3 section leaf production,
The whole strain sample of wheat it is not, this is because lacking the glass for being suitble to wheat strain preparation of specimen on volume and height in the market
Container, such as glass container height highest in the market less than 50cm, and wheat plant only aerial part usually just in 80cm or so,
The plant height of some Wheat Germplasm Resources is in 1m or more, so glass container in the market is unable to satisfy wheat strain sample
Production.It there is no the liquid for seeing wheat root humid preparation itself and the wheat strain including aerial part organ at present
Humid preparation.
Summary of the invention
To solve the deficiency in the prior art, the present invention provides one kind to take wheat plant, root system from field soil
Flushing, removal root system sundries, whole strain chemical treatment, removal treatment fluid, formaldehyde liquid immersion, sample shaping, transparent acrylic have
The production method for the wheat plant liquid humid preparation that machine glass container is sealed.The present invention be adapted to wheat crops (including
Transgenic line) under all soil cultivation conditions and under non-soil cultivation condition such as tissue culturing seedling and whole plant or only
The production of the root system partially liq humid preparation of underground.
The present invention is achieved by the following technical solutions:
1) candidate plant is selected
In the field plots of plantation wheat, candidate plant of the eugonic plant as production sample is chosen, and is listed
Label, then pulls out other unwanted plant shoots；3 or more candidate plant are chosen, plant spacing will be remained above or wait
In 70 cm；Or in wheat cultivation, wheat cultivation spacing is remained above or is equal to 70 cm；
2) earthwork is selected
It using wheat candidate plant as the center of circle, draws and justifies according to 30cm radius, be vertically to soil layer insertion diameter in circumference
The reinforcing bar of 0.5cm, a length of 1m, bar spacing 10cm or so；
3) wheat plant takes
Around the reinforcing bar periphery of insertion soil layer, using shoveling tool, take the cylinder that internal diameter is 30cm, a height of 1m or more
Cubic metre of earth；Meanwhile in cylindrical earthwork periphery opposite sides, there are 2 opposite, internal diameter 30cm or more, deep about 40cm work
Level, in favor of subsequent job operation；
4) punching is native and washes root
Using water injector, gradual flushing is carried out to the cylindrical earthwork；When rinsing, water flow cannot be too strong, in order to avoid cause
Large clod is fallen, and the root system of attachment is broken；The soil layer of cylindrical earthwork 30-40cm is relatively soft, should slowly rinse, and rinses
As a result, should be that soil layer above the cylindrical earthwork gradually tails off, soil layer below is slightly more, form an equilateral triangle structure,
Otherwise mound is easy to collapse；Cylindrical earthwork bottom solum relative rigid, with the water soaked overnight of about 50cm depth, while with wet
Gauze wraps up the exposed root system in top, in case root system is dry and damages；Cylindrical earthwork bottom soil block, guarantee root system not by
When damage, it can suitably increase flow intensity；Extra water flow and mud can be taken out by reserved working interface；
5) taking-up of wheat root mound is had
Wheat plant root system is rushed to mound with water there are about when 10-15kg weight, the muddy water in hole is all taken out, and into hole
Landfill dry ground is to being able to bear certain human body weight；In hole, using tools such as scuppits from mound bottom, slowly dug
Pick finally makes mound bottom be detached from soil；Mound with root system is gently put in plastic box, and is ajusted, then infuses water
It is full, it is dipped to soil block and fades to muddy；
6) root is washed
It is clear to water that root system is gently rinsed using tap water；When rinsing, to small clod, before guaranteeing that root system is injury-free
It puts, is gently pulverized with hand in water；
7) sundries on root is removed
The wheat plant of acquisition is placed in the plastic tub containing clear water, root system will using small-sized tweezers or be manually attached to
On the sundries removal such as rotten weeds it is clean, and ajust root system gently as growth posture, prepare for the processing of sample subsequent chemistry；
8) plant copper acetate is heat-treated
When copper acetate treatment fluid is heated to 85 DEG C, pour into 120 cm long, 25 cm wide, 25 cm high acrylic transparent have
In machine glass guide channel, it is then placed in the clean fresh wheat plant of washing and is chemically treated；During chemical treatment, pass through heater
Treatment fluid is constantly heated, makes liquid temperature control system at 80 DEG C or so, material processing needs 15 min or so, and wheat plant is by green
Color gradually turns yellow, browning, last and become taking out material when green, and is rinsed 2-3 times with clear water to processed material；Processing
Process will be carried out in ventilation, and often stir wheat plant, so that its uniform coloring；
9.) the fixation shaping of sample
5% formalin is prepared, and is injected in the cylindrical transparent acrylic specimen jar of 20 cm of diameter, high 120 cm；Benefit
The upper end that plant root is tied down with 2 symmetrical setlines or white Nylon line, then since the lower end of plant root, by sample
Slowly it is put into and fills in the specimen jar for saving liquid, make to save liquid submergence sample；Plant posture is adjusted, its root system is made to be in vertical configuration
Then state fixes sample；Specimen jar lid is coated into vaseline, specimen jar mouth is then covered and is sealed sealing, be placed in
Shady place long-term preservation；During preservation, if saving liquid turbid phenomenon occurs, the formaldehyde that should more renew at once saves liquid.
The 1) in step, the candidate plant it is selected, before period is preferably wheat period of seedling establishment.
The 2) in step, the earthwork it is selected, period is preferably wheat during jointing stage.
The 8) in step, the plant copper acetate heat treatment, the preparation method of the copper acetate treatment fluid are as follows: according to 180 g
Copper acetate, 500 ml glacial acetic acid, 500 ml ratio of water are configured to saturated solution, then this saturated solution is diluted 5 times.
The beneficial effects of the present invention are:
1, the present invention provides to the wheat root method of sampling, the method is small to wheat root damage, is conducive to save root
The natural growth conditions of system.
2, the present invention is conducive to carry out long-term preservation and research to wheat root.
3, the present invention can according to the different height and size of wheat, adjust save sample transparent organic glass container and
The organic glass slot of sample disposal, convenient for different height of the production including complete root system and the entire wheat plant mark of size
This.
Detailed description of the invention
Fig. 1 wheat plant takes.
Fig. 2 punching is native and washes root.
Fig. 3 rinses the wheat plant root system taken.
Fig. 4 removes the sundries on wheat root and arranges to root system.
Fig. 5 carries out copper acetate heat treatment to wheat plant.
Fig. 6 clear water rinses processed wheat lines.
Fig. 7 prepares formalin and injects in cylindrical transparent acrylic specimen jar.
The wheat plant liquid humid preparation that Fig. 8 completes.
Specific embodiment
Further detailed description is done to the present invention below with reference to embodiment:
The production of 1 wheat sample of embodiment
In Shandong Academy of Agricultural Sciences, experimental farm, Jinan, wheat planting and sampling are carried out, makes sample.
1. plant is selected
In the field plots of plantation wheat, before wheat period of seedling establishment, eugonic plant is chosen as production sample
Then candidate plant, and listing mark pull out other unwanted plant shoots.It is general to choose 3 candidate plant, plant circumference
Spacing will keep 70 cm；Or in wheat cultivation, wheat cultivation spacing keeps 70 cm.
2. cubic metre of earth it is selected
In wheat during jointing stage, around the wheat candidate plant selected, diameter vertically is inserted into soil layer using 30cm as radius
The 1m long reinforcing bar of 0.5cm, bar spacing 10cm or so (Fig. 1).
3. wheat plant takes
Around the reinforcing bar periphery of insertion, using tools such as spade, pick, spades, take internal diameter 30cm, depth 1m or more
Soil layer.Meanwhile outside original internal diameter 30cm, there are 2 opposite, internal diameter 30cm or more, deep about 40cm working surfaces, with benefit
In to the digging out of bottom solum, subsequent extra muddy water take and the taking-up (Fig. 1) of entire plant and root system.
4. punching is native and washes root
The agricultural water injector or piston type children toy water gun being equipped with using driven high voltage hydraulic giant or tractor, to selected
The soil layer of plant carries out gradual flushing.Make sure to keep in mind, when rinsing, water flow cannot be too strong, in order to avoid falling for large clod is caused, it will be attached
Root system break.The soil layer of 30-40cm is relatively soft, should slowly rinse, flushing as a result, should be that soil layer above tails off,
Following soil layer is slightly more, forms an equilateral triangle structure, otherwise mound is easy to collapse.Bottom solum relative rigid, with pouring
The water soaked overnight of about 50cm depth, while being wrapped up the exposed root system in mound top with wet gauze, in case root system is dry and damages
Wound.Flow intensity can suitably be increased when guaranteeing that root system is injury-free to bottom soil block.Extra water flow and mud, can be with
By reserved working interface, (Fig. 2) is taken out repeatedly by vessel such as beaker, spades.
5. having the taking-up of wheat root mound
It rushes to mound when wheat plant root system there are about when 10-15kg weight, the muddy water in hole is all taken out, and is filled out into hole
Dry ground is buried to being able to bear certain human body weight.In hole, using tools such as scuppits from mound bottom, slowly excavated,
Finally separate mound with bottom.Mound with root system is gently put in plastic box, and is ajusted, then fills water, is soaked
Bubble to soil block fades to muddy.
6. washing root
After the clod in mound is dipped to mud, gently rinsed using tap water clear to water.When rinsing, to small mud
Block is gently pulverized (Fig. 3) with hand in water under the premise of guaranteeing that root system is injury-free.
7. removing the sundries on root
The wheat plant of acquisition is placed in the plastic tub containing clear water, root system will using small-sized tweezers or be manually attached to
On the sundries removal such as rotten weeds it is clean, and gently ajust root growth posture, prepare (Fig. 4) for specimen staining.
8. plant copper acetate is heat-treated
First by 180 g copper acetates, 500 ml glacial acetic acid, 500 ml of water is added to stir into saturated solution, then this saturated solution is dilute
Releasing 5 times is material processing liquid.When treatment fluid is heated to 85 DEG C, the acrylic of 120 cm long, 25 cm wide, 25 cm high are poured into
In transparent organic glass slot, it is then placed in the clean fresh wheat plant of washing and is chemically treated (Fig. 5).The chemical treatment phase
Between, treatment fluid is constantly heated by heater, makes liquid temperature control system at 80 DEG C or so, material processing needs 15 min, to
Wheat plant from green, gradually take out material, and floated with clear water to processed material by flavescences, browning, last and while becoming green
It washes 2-3 times (Fig. 6).Pay attention to carrying out in ventilation during processing, and often stir material, so that its uniform coloring.
9. the fixation shaping of sample
5% formalin is prepared, and injects in the cylindrical transparent acrylic specimen jar of 20 cm of diameter, high 120 cm (figure
7).The upper end that plant root is tied down using 2 symmetrical setlines or white Nylon line, then since the lower end of plant root,
Sample is blown slowly to be put into and is filled in the specimen jar for saving liquid, does not cross sample.Plant posture is adjusted, its root system is made to be in vertical configuration
Then state fixes sample.Specimen jar lid is coated into vaseline, specimen jar mouth is then covered and is sealed sealing, be placed in
Shady place long-term preservation (Fig. 8).During preservation, if saving liquid turbid phenomenon occurs, the formaldehyde that should more renew at once is saved
Liquid.
The above is only the preferred embodiment of this patent, it is noted that for the ordinary skill people of the art
For member, under the premise of not departing from the art of this patent principle, several improvement and replacement can also be made, these are improved and replacement
Also it should be regarded as the protection scope of this patent.
Claims (1)
1. a kind of production method of wheat plant liquid humid preparation, comprising the following steps:
1) candidate wheat plant is selected
Before wheat period of seedling establishment, in the field plots of plantation wheat, chooses eugonic plant and planted as the candidate of production sample
Strain, and listing mark, then pull out other unwanted plant shoots；3 or more candidate plant are chosen, plant spacing will be kept
More than or equal to 70 cm；Or in wheat cultivation, wheat cultivation spacing is remained above or is equal to 70 cm；
2) earthwork is selected
Wheat during jointing stage is drawn according to 30cm radius and is justified using wheat candidate plant as the center of circle, is vertically inserted into diameter to soil layer in circumference
For the reinforcing bar of 0.5cm, a length of 1m, bar spacing 10cm；
3) wheat plant takes
Take internal diameter using shoveling tool around the reinforcing bar periphery of insertion soil layer for the cylinder soil of 30cm, a height of 1m or more
Side；Meanwhile in cylindrical earthwork periphery opposite sides, there are 2 opposite, internal diameter 30cm or more, depth 40cm working surfaces,
In favor of subsequent job operation；
4) punching is native and washes root
Using water injector, gradual flushing is carried out to the cylindrical earthwork；When rinsing, water flow cannot be too strong, in order to avoid cause big soil
Block is fallen, and the root system of attachment is broken；The soil layer of cylindrical earthwork 30-40cm is relatively soft, should slowly rinse, the knot of flushing
Fruit should be that the soil layer above the cylindrical earthwork gradually tails off, and soil layer below is slightly more, forms an equilateral triangle structure, otherwise
Mound is easy to collapse；Cylindrical earthwork bottom solum relative rigid, with the water soaked overnight of 50cm depth, while will with wet gauze
The exposed root system package in top, in case root system is dry and damages；Cylindrical earthwork bottom soil block is guaranteeing that root system is injury-free
When, it can suitably increase flow intensity；Extra water flow and mud can be taken out by reserved working interface；
5) taking-up of wheat root mound is had
Wheat plant root system is rushed with water when having 10-15kg weight to mound, the muddy water in hole is all taken out, and fill and do into hole
Soil is to being able to bear certain human body weight；In hole, using scuppit tool from mound bottom, is slowly excavated, finally made
Mound bottom is detached from soil；Mound with root system is gently put in plastic box, and is ajusted, then water is filled, is dipped to
Soil block fades to muddy；
6) root is washed
It is clear to water that root system is gently rinsed using tap water；When rinsing, to small clod, guaranteeing the injury-free premise of root system
Under, it is gently pulverized with hand in water；
7) sundries on root is removed
The wheat plant of acquisition is placed in the plastic tub containing clear water, will using small-sized tweezers or be manually attached on root system
Rotten weeds and the removal of other sundries are clean, and ajust root system gently to grow posture, prepare for the processing of sample subsequent chemistry；
8) plant copper acetate is heat-treated
It prepares copper acetate treatment fluid: being configured to saturated solution according to 180 g copper acetates, 500 ml glacial acetic acid, 500 ml ratio of water,
This saturated solution is diluted 5 times again；When copper acetate treatment fluid is heated to 85 DEG C, 120 cm long, 25 cm wide, 25 cm high are poured into
In acrylic transparent organic glass slot, it is then placed in the clean fresh wheat plant of washing and is chemically treated；The chemical treatment phase
Between, treatment fluid is constantly heated by heater, makes liquid temperature control system at 80 DEG C, material processing needs 15 min, works as wheat
Plant from green gradually flavescences, browning, it is last become green again when take out material, and with clear water to processed material rinsing 2-
3 times；Treatment process will be carried out in ventilation, and often stir wheat plant, so that its uniform coloring；
9) the fixation shaping of sample
5% formalin is prepared, and is injected in the cylindrical transparent acrylic specimen jar of 20 cm of diameter, high 120 cm；Utilize 2
Then sample is blown slowly since the lower end of plant root the upper end that symmetrical setline or white Nylon line tie down plant root
It is put into and fills in the specimen jar for saving liquid, make to save liquid submergence sample；Plant posture is adjusted, its root system is made to be in plumbness,
Then sample is fixed；Specimen jar lid is coated into vaseline, specimen jar mouth is then covered and is sealed sealing, be placed in cool place
Locate long-term preservation；During preservation, if saving liquid turbid phenomenon occurs, the formaldehyde that should more renew at once saves liquid.
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| CN201610639019.XA CN106359369B (en) | 2016-08-08 | 2016-08-08 | A kind of production method of wheat plant liquid humid preparation |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108353892A (en) * | 2018-01-05 | 2018-08-03 | 湖南农业大学 | The production method of rice plant sample |
| CN110447423A (en) * | 2019-08-26 | 2019-11-15 | 河北省农林科学院旱作农业研究所 | A kind of millet or wheat root preparation of specimen device and method |
| CN113207873A (en) * | 2021-05-19 | 2021-08-06 | 石家庄市农林科学研究院 | Method for preparing primary color wheat soaked specimen |
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| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
| CN101401567A (en) * | 2008-03-19 | 2009-04-08 | 中国科学院新疆生态与地理研究所 | Eremophyte steeping specimen and method for making protective color |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
| CN103392694A (en) * | 2013-08-26 | 2013-11-20 | 中国医学科学院药用植物研究所海南分所 | Method for preparing tropical plant preserving humid preparation |
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2016
- 2016-08-08 CN CN201610639019.XA patent/CN106359369B/en active Active
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|---|---|---|---|---|
| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
| CN101401567A (en) * | 2008-03-19 | 2009-04-08 | 中国科学院新疆生态与地理研究所 | Eremophyte steeping specimen and method for making protective color |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
| CN103392694A (en) * | 2013-08-26 | 2013-11-20 | 中国医学科学院药用植物研究所海南分所 | Method for preparing tropical plant preserving humid preparation |
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