CN107018976A - Reddish yellow dichromatism plant disease sample primary color preservation method - Google Patents
Reddish yellow dichromatism plant disease sample primary color preservation method Download PDFInfo
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- CN107018976A CN107018976A CN201710293014.0A CN201710293014A CN107018976A CN 107018976 A CN107018976 A CN 107018976A CN 201710293014 A CN201710293014 A CN 201710293014A CN 107018976 A CN107018976 A CN 107018976A
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- distilled water
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Abstract
The present invention provides a kind of reddish yellow dichromatism plant disease sample primary color preservation method, and step is as follows：1）Plant disease collection of specimens arranges sterilizing；2）Color is fixed；3）Color is repaired；4）Primary color preservation.The present invention original creation color fix, color reparation, three color treatments steps of primary color preservation, two kinds of color samples can be just preserved using a kind of method.Materials safety environmental protection, method is simple and easy to apply, time saving and energy saving, with adopt with system, can batch making, for complete specimen original shape primary color preservation, preserve that liquid is Clear ＆ Transparent, the application in Plant Pathology teaching and scientific research field has positive realistic meaning.
Description
Technical field
The invention belongs to Plant Pathology field, and in particular to reddish yellow dichromatism plant disease sample primary color preservation method.
Background technology
Reddish yellow dichromatism plant disease sample is one of important component in plant disease sample.Reddish yellow dichromatism phytopathy
Evil sample has many differences with green sample in preservation, and green sample is pure color, and accounting example weight, research dynamics is larger, mesh
Preceding Technical comparing is ripe.Red Yellow disease sample is substantially fruit type sample, complete a red, yellow plant disease sample,
Often the fruit of blade, carpopodium, base of fruit, fruit branch with green portion etc., will accomplish that the primary color preservation of complete specimen is necessary
Accomplish to preserve while multiple color.The Techniques of preserving research for specializing in reddish yellow dichromatism plant disease sample at present is few, generally adopts
Liquid is preserved with one kind directly to preserve, guarantor's color effect is not good, and sample color and luster and fresh specimens difference are excessive, easy dehiscent fruit.Some are protected
Contain formaldehyde components in color effect slightly good method, formula, it is well known that formaldehyde is poisonous and harmful substance, and being unfavorable for making makes
User's is healthy.
For the realistic problem of current Plant Pathology teaching and scientific research field plant disease Saving specimen, how one kind is designed
Reddish yellow dichromatism plant disease sample primary color preservation method, can preserve two kinds of color samples, and simple and easy to apply, safety and environmental protection,
With positive realistic meaning.
The content of the invention
The purpose of the present invention aims to provide a kind of reddish yellow dichromatism plant disease sample primary color preservation method, store method operation
Simply, safety and environmental protection, reddish yellow dichromatism disease sample original shape primary color preservation.
To achieve the above object, reddish yellow dichromatism plant disease sample primary color preservation method of the present invention, step is as follows：
1）Plant disease collection of specimens arranges sterilizing：Gathering 7-8 maturations has single, classical symptom, with blade, carpopodium, fruit
The Red Yellow fruit disease sample of the base of a fruit, fruit branch, trimming cleans complete specimen with clear water after putting in order, is put into 70% alcohol
Middle soaking disinfection 3-4 minutes, cleaned 3-4 times with distilled water；
2）Color is fixed：The sample for sterilizing cleaned is put into fixer, the space that sample accounts for fixer is no more than 80%, mark
Originally to be completely submerged in fixer, sample green portion is turned yellow by green after 5-10 days, gradually after green 1~2 day of xanthochromia, is taken out
Rinsed with clear water；
3）Color is repaired：The sample rinsed, which is put into 70% alcohol, to be soaked 2-3 minutes, and taking-up distilled water, which is cleaned, to be dried, mark
Originally it is put into complex color liquid and preserves 12-15 days；
4）Primary color preservation：Sample is taken out from complex color liquid, is put into the good specimen bottle of sterilization, sample is arranged by nature
Put well, 1-2 duck eye is cut off with dissecting scissors at carpopodium for hollow sample, then preserve liquid, liquid toward injection in specimen bottle
At identity distance bottleneck 2cm, it is exhausted during preservation liquid immersed specimen, exhaust is added and preserves liquid to keep liquid level again after 1 day
At bottleneck 2cm, sealed after 1-2 days in the case where air humidity is the environmental condition within 70% with sealing solution, sample is put
Preserved for a long time into the clean condition that air humidity is 70%-80% lucifuges.
Further, the fixer is made up of following raw material：15~25 grams of copper sulphate, 40~60ml acetic acid, 50~
80ml glycerine, 15~20ml sulfurous acid, 15~25 grams of copper sulphate is are put into 800ml distilled water by preparation method, and stirring is extremely
Copper sulphate is completely dissolved, and adds acetic acid, glycerine and sulfurous acid, is finally added distilled water to be settled to 1000 ml and is produced fixation
Liquid.
Further, the complex color liquid is made up of following raw material：13~16ml sulfurous acid, 80~100ml glycerine, 4~5
Gram boric acid, 4~5 grams of boric acid is are put into 150ml distilled water by preparation method, and stirring is completely dissolved to boric acid, adds sulfurous
Acid and glycerine, finally add distilled water to be settled to 1000ml and produce complex color liquid.
Further, the complex color liquid is made up of following raw material：13~16ml sulfurous acid, 80~100ml glycerine, 80~
The alcohol of 100ml 95%, preparation method is after mixing sulfurous acid, glycerine, alcohol, finally to add distilled water to be settled to 1000ml i.e.
Obtain complex color liquid.
Further, the preservation liquid is made up of following raw material：10~12ml sulfurous acid, 50~70ml glycerine ml, 4~
5 grams of boric acid, 4~5 grams of boric acid is are put into 150ml distilled water by preparation method, and stirring is completely dissolved to boric acid, adds sulfurous
Acid and glycerine, finally add distilled water to be settled to 1000ml and produce preservation liquid.
Further, the preservation liquid is made up of following raw material：10~12ml sulfurous acid, 50~70ml glycerine, 50~
The alcohol of 80ml 95%, preparation method is：After sulfurous acid, glycerine, alcohol are mixed, finally distilled water is added to be settled to 1000ml i.e.
Liquid must be preserved.
The present invention substantive distinguishing features and marked improvement be：
The reddish yellow dichromatism plant disease sample primary color preservation method of the present invention, original creation color is fixed, color reparation, primary color preservation
Three color treatments steps, two kinds of color samples can be just preserved using a kind of method.Materials safety environmental protection, preparation method is simple
It is easy, time saving and energy saving, with adopt with system, can batch making, for complete specimen original shape primary color preservation, it is Clear ＆ Transparent to preserve liquid,
The application in Plant Pathology teaching and scientific research field has positive realistic meaning.
Embodiment
With reference to embodiment, the present invention is described in detail.
Embodiment one：
Reddish yellow dichromatism plant disease sample primary color preservation method of the present invention, for the preservation of yellow loquat anthracnose, specific steps
For：
1）The collection of specimens of loquat anthracnose arranges sterilizing.Gather single, classical symptom the loquat anthrax mark of bunchiness 7-8 maturation tools
This, removes unnecessary old leaf, other disease fruits, leaves the normal fruit of disease fruit and health of classical symptom.With fruit branch, carpopodium,
Base of fruit, disease fruit, the complete specimen of healthy fruit.Trimming cleans up complete specimen with clear water after putting in order, is put into 70% wine
Soaking disinfection 3-4 minutes in essence, cleaned 3-4 times with distilled water.
2）Color is fixed.The loquat anthracnose sample for sterilizing cleaned is put into fixer, sample accounts for the sky of fixer
Between be no more than 80%, sample will be completely immersed in fixer.Sample green portion is turned yellow by green after 5 days, gradually green 2 days by xanthochromia
Afterwards, taking-up is rinsed with clear water.
The preparation of fixer：15 grams of copper sulphate are put into 800ml distilled water, stirring to copper sulphate is completely dissolved, then added
Enter 40ml acetic acid, 50ml glycerine and 15ml sulfurous acid, finally add distilled water to be settled to 1000 ml and produce fixer.
3）Color is repaired.The sample rinsed, which is put into 70% alcohol, to be soaked 2-3 minutes, is cleaned taking-up with distilled water and is dried,
Sample is put into complex color liquid and preserved 15 days.
The preparation of complex color liquid：15ml sulfurous acid, 100ml glycerine, 95% alcohol 100ml are added in 300ml distilled water and mixed
Close, finally plus distilled water is settled to 1000ml and stirred and produces complex color liquid.
4）Primary color preservation.Loquat anthracnose sample is taken out from complex color liquid, is put into the good specimen bottle of sterilization, is marked
This is discharged by nature, cuts off 1 to 2 duck eyes with dissection near carpopodium, then preserves liquid, liquid toward injection in specimen bottle
At identity distance bottleneck 2cm, unavoidably contain gas in sample, be exhausted during liquid immersed specimen is preserved, after being vented 1 day
Again addition preserve liquid with keep liquid level away from bottleneck 2cm at.In the case where air humidity is the environmental condition within 70% with envelope after 2 days
Mouth agent is sealed, and the formula of sealing compound is beeswax, each 1 part of vaseline.Finally, sample is put into humidity for 70% to 80% lucifuge
Clean condition in preserve for a long time.
Preserve the preparation of liquid：10ml sulfurous acid, 70ml glycerine, 95% alcohol 60ml are added in 300ml distilled water and mixed
Close, finally plus distilled water is settled to 1000ml and stirred and produces preservation liquid.
Embodiment two：
Reddish yellow dichromatism plant disease sample primary color preservation method of the present invention, for the preservation of red tomatoes late blight, specific steps
For：
1）Tomato late blight collection of specimens arranges sterilizing.Single, classical symptom the tomato late blight of bunchiness 7-8 maturation tools is gathered,
Unnecessary old leaf, other disease fruits are removed, the normal fruit of disease fruit and health of classical symptom is left.With blade, fruit branch, fruit
Handle, base of fruit, disease fruit, the complete specimen of healthy fruit.Trimming cleans up complete specimen with clear water after putting in order, is put into 70%
Alcohol in soaking disinfection 3-4 minutes, cleaned 3-4 times with distilled water.
2）Color is fixed.The tomato late blight sample for sterilizing cleaned is put into fixer, sample accounts for the sky of fixer
Between be no more than 80%, sample will be completely immersed in fixer.Sample green portion is turned yellow by green after 6 days, gradually green 2 days by xanthochromia
Afterwards, taking-up is rinsed with clear water.
The preparation of fixer：20 grams of copper sulphate are put into 800ml distilled water, stirring to copper sulphate is completely dissolved, then added
Enter 50ml acetic acid, 65ml glycerine and 15ml sulfurous acid, finally add distilled water to be settled to 1000 ml and produce fixer.
3）Color is repaired.The sample rinsed, which is put into 70% alcohol, to be soaked 2-3 minutes, is cleaned taking-up with distilled water and is dried,
Sample is put into complex color liquid and preserved 15 days.
The preparation of complex color liquid：15ml sulfurous acid, 100ml glycerine, 95% alcohol 100ml are added in 300ml distilled water and mixed
Close, finally plus distilled water is settled to 1000ml and stirred and produces complex color liquid.
4）Primary color preservation.Tomato late blight sample is taken out from complex color liquid, is put into the good specimen bottle of sterilization, is marked
This discharges by nature, then preserves liquid toward injection in specimen bottle, liquid level away from bottleneck 2cm at, unavoidably contain in sample
There is gas, be exhausted during liquid immersed specimen is preserved, exhaust is added and preserves liquid to keep liquid level away from bottleneck again after 1 day
At 2cm.Sealed after 2 days in the case where air humidity is the environmental condition within 70% with sealing compound, the formula of sealing compound is honeybee
Each 1 part of wax, vaseline.Finally, sample is put into the clean condition that humidity is 70% to 80% lucifuge and preserved for a long time.
Preserve the preparation of liquid：10ml sulfurous acid, 70ml glycerine, 95% alcohol 60ml are added in 300ml distilled water and mixed
Close, finally plus distilled water is settled to 1000ml and stirred and produces preservation liquid.
Embodiment three：
Reddish yellow dichromatism plant disease sample primary color preservation method of the present invention, for the preservation of red and yellow pimento anthracnose, tool
Body step is：
1）The collection of specimens of pimento anthracnose arranges sterilizing.Collection 7-8 maturations have single, classical symptom red and yellow sweet tea respectively
Green pepper anthracnose disease fruit, selects the normal fruit of disease fruit and health of classical symptom, with blade, carpopodium, base of fruit, disease fruit, health
The complete specimen of fruit.Trimming cleans up complete specimen with clear water after putting in order, is put into soaking disinfection 3- in 70% alcohol
4 minutes, cleaned 3-4 times with distilled water.
2）Color is fixed.The pimento anthracnose sample for sterilizing cleaned is put into fixer, sample accounts for the sky of fixer
Between be no more than 80%, sample will be completely immersed in fixer.Sample green portion is turned yellow by green after 5 days, gradually green 2 days by xanthochromia
Afterwards, taking-up is rinsed with clear water.
The preparation of fixer：25 grams of copper sulphate are put into 800ml distilled water, stirring to copper sulphate is completely dissolved, then added
Enter 60ml acetic acid, 80ml glycerine and 20ml sulfurous acid, finally add distilled water to be settled to 1000 ml and produce fixer.
3）Color is repaired.The sample rinsed, which is put into 70% alcohol, to be soaked 2-3 minutes, is cleaned taking-up with distilled water and is dried,
Sample is put into complex color liquid and preserved 15 days.
The preparation of complex color liquid：5 grams of boric acid are put into 150ml distilled water, stirring is completely dissolved to boric acid, adds 15ml
Sulfurous acid and 100ml glycerine, finally add distilled water to be settled to 1000ml and produce complex color liquid.
4）Primary color preservation.Red and yellow pimento anthracnose sample is taken out from complex color liquid, while being put into sterilization
In good specimen bottle, sample is discharged by nature, then preserves liquid toward injection in specimen bottle, and liquid level is located away from bottleneck 2cm, marks
Unavoidably contain gas in this, be exhausted during liquid immersed specimen is preserved, after exhaust 1 day again addition preservation liquid with
Keep liquid level away from bottleneck 2cm at.Sealed, sealed with sealing compound in the case where air humidity is the environmental condition within 70% after 2 days
The formula of agent is beeswax, each 1 part of vaseline.Finally, sample is put into long-term in the clean condition that humidity is 70% to 80% lucifuge
Preserve.
Preserve the preparation of liquid：4 grams of boric acid are put into 150ml distilled water, stirring is completely dissolved to boric acid, adds 10ml
Sulfurous acid and 70ml glycerine, finally add distilled water to be settled to 1000ml and produce preservation liquid.
Preferred embodiment of the invention described in detail above.It should be appreciated that the ordinary skill of this area is without wound
The property made work just can make many modifications and variations according to the design of the present invention.Therefore, all technical staff in the art
Pass through the available technology of logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Scheme, all should be in the protection domain being defined in the patent claims.
Claims (6)
1. a kind of reddish yellow dichromatism plant disease sample primary color preservation method, it is characterised in that step is as follows：
1）Plant disease collection of specimens arranges sterilizing：Gathering 7-8 maturations has single, classical symptom, with blade, carpopodium, fruit
The Red Yellow fruit disease sample of the base of a fruit, fruit branch, trimming cleans complete specimen with clear water after putting in order, is put into 70% alcohol
Middle soaking disinfection 3-4 minutes, cleaned 3-4 times with distilled water；
2）Color is fixed：The sample for sterilizing cleaned is put into fixer, the space that sample accounts for fixer is no more than 80%, mark
Originally to be completely submerged in fixer, sample green portion is turned yellow by green after 5-10 days, gradually after green 1~2 day of xanthochromia, is taken out
Rinsed with clear water；
3）Color is repaired：The sample rinsed, which is put into 70% alcohol, to be soaked 2-3 minutes, and taking-up distilled water, which is cleaned, to be dried, mark
Originally it is put into complex color liquid and preserves 12-15 days；
4）Primary color preservation：Sample is taken out from complex color liquid, is put into the good specimen bottle of sterilization, sample is arranged by nature
Put well, 1-2 duck eye is cut off with dissecting scissors at carpopodium for hollow sample, then preserve liquid, liquid toward injection in specimen bottle
At identity distance bottleneck 2cm, it is exhausted during preservation liquid immersed specimen, exhaust is added and preserves liquid to keep liquid level again after 1 day
At bottleneck 2cm, sealed after 1-2 days in the case where air humidity is the environmental condition within 70% with sealing solution, sample is put
Preserved for a long time into the clean condition that air humidity is 70%-80% lucifuges.
2. reddish yellow dichromatism plant disease sample primary color preservation method according to claim 1, it is characterised in that：The fixation
Liquid is made up of following raw material：15~25 grams of copper sulphate, 40~60ml acetic acid, 50~80ml glycerine, 15~20ml sulfurous acid,
15~25 grams of copper sulphate is are put into 800ml distilled water by preparation method, and stirring is completely dissolved to copper sulphate, add acetic acid,
Glycerine and sulfurous acid, finally add distilled water to be settled to 1000 ml and produce fixer.
3. reddish yellow dichromatism plant disease sample primary color preservation method according to claim 1, it is characterised in that：The secondary color
Liquid is made up of following raw material：13~16ml sulfurous acid, 80~100ml glycerine, 4~5 grams of boric acid, preparation method are by 4~5 grams
Boric acid is put into 150ml distilled water, and stirring is completely dissolved to boric acid, adds sulfurous acid and glycerine, finally adds distilled water to determine
Hold to 1000ml and produce complex color liquid.
4. reddish yellow dichromatism plant disease sample primary color preservation method according to claim 1, it is characterised in that：The secondary color
Liquid is made up of following raw material：13~16ml sulfurous acid, 80~100ml glycerine, the alcohol of 80~100ml 95%, preparation method is
Sulfurous acid, glycerine, alcohol are added in 300ml distilled water and mixed, finally adds distilled water to be settled to 1000ml and stirs i.e.
Obtain complex color liquid.
5. reddish yellow dichromatism plant disease sample primary color preservation method according to claim 1, it is characterised in that：It is described to preserve
Liquid is made up of following raw material：10~12ml sulfurous acid, 50~70ml glycerine ml, 4~5 grams of boric acid, preparation method are by 4~5
Gram boric acid is put into 150ml distilled water, and stirring is completely dissolved to boric acid, adds sulfurous acid and glycerine, finally plus distilled water
It is settled to 1000ml and produces preservation liquid.
6. reddish yellow dichromatism plant disease sample primary color preservation method according to claim 1, it is characterised in that：It is described to preserve
Liquid is made up of following raw material：10~12ml sulfurous acid, 50~70ml glycerine, the alcohol of 50~80ml 95%, preparation method is：Will
Sulfurous acid, glycerine, alcohol are added in 300ml distilled water and mixed, finally plus distilled water is settled to 1000ml and stirred and produces
Preserve liquid.
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|---|---|---|---|
| CN201710293014.0A CN107018976B (en) | 2017-04-28 | 2017-04-28 | Primary color preservation method for red and yellow plant disease specimen |
Applications Claiming Priority (1)
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|---|---|---|---|
| CN201710293014.0A CN107018976B (en) | 2017-04-28 | 2017-04-28 | Primary color preservation method for red and yellow plant disease specimen |
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| CN107018976B CN107018976B (en) | 2020-12-08 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077246A (en) * | 2017-12-25 | 2018-05-29 | 重庆尔麟农业开发有限公司 | A kind of production method of flower specimen |
| CN108990968A (en) * | 2018-09-25 | 2018-12-14 | 河南科技大学 | A kind of production method of the biological plasticized sample of Alfalfa primary colors overall picture |
| CN109221095A (en) * | 2018-09-26 | 2019-01-18 | 胡玥瑶 | A kind of production method of green plants humid preparation |
| CN109699638A (en) * | 2019-01-22 | 2019-05-03 | 河北出入境检验检疫局检验检疫技术中心 | A kind of production method that the operatic circle dipping specimen saves liquid and dipping specimen |
| CN113545339A (en) * | 2021-07-12 | 2021-10-26 | 中国林业科学研究院亚热带林业研究所 | Preparation method of bamboo shoot soaked specimen |
| CN114680103A (en) * | 2022-04-15 | 2022-07-01 | 西北农林科技大学 | Method for preparing plant disease specimen |
| CN114982745A (en) * | 2022-04-29 | 2022-09-02 | 濮阳市食品药品检验检测中心 | Method for preparing plant-soaked specimen |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077246A (en) * | 2017-12-25 | 2018-05-29 | 重庆尔麟农业开发有限公司 | A kind of production method of flower specimen |
| CN108990968A (en) * | 2018-09-25 | 2018-12-14 | 河南科技大学 | A kind of production method of the biological plasticized sample of Alfalfa primary colors overall picture |
| CN109221095A (en) * | 2018-09-26 | 2019-01-18 | 胡玥瑶 | A kind of production method of green plants humid preparation |
| CN109699638A (en) * | 2019-01-22 | 2019-05-03 | 河北出入境检验检疫局检验检疫技术中心 | A kind of production method that the operatic circle dipping specimen saves liquid and dipping specimen |
| CN109699638B (en) * | 2019-01-22 | 2021-01-12 | 河北出入境检验检疫局检验检疫技术中心 | Pear dipped specimen preserving fluid and manufacturing method of dipped specimen |
| CN113545339A (en) * | 2021-07-12 | 2021-10-26 | 中国林业科学研究院亚热带林业研究所 | Preparation method of bamboo shoot soaked specimen |
| CN114680103A (en) * | 2022-04-15 | 2022-07-01 | 西北农林科技大学 | Method for preparing plant disease specimen |
| CN114680103B (en) * | 2022-04-15 | 2023-03-14 | 西北农林科技大学 | Method for preparing plant disease specimen |
| CN114982745A (en) * | 2022-04-29 | 2022-09-02 | 濮阳市食品药品检验检测中心 | Method for preparing plant-soaked specimen |
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