CN107114178A - A kind of Piao Shu cuttage seedling raising method - Google Patents
A kind of Piao Shu cuttage seedling raising method Download PDFInfo
- Publication number
- CN107114178A CN107114178A CN201710227366.6A CN201710227366A CN107114178A CN 107114178 A CN107114178 A CN 107114178A CN 201710227366 A CN201710227366 A CN 201710227366A CN 107114178 A CN107114178 A CN 107114178A
- Authority
- CN
- China
- Prior art keywords
- cutting
- cuttage
- callus
- ointment
- branch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of Piao Shu cuttage seedling raising method, following steps are specifically included:(1)The screening of cutting;(2)The clip of cutting;(3)The processing of cutting;(4)The cuttage of cutting.By experiment, a kind of Piao Shu provided using the present invention cuttage seedling raising method substantially increases the rooting rate of Piao Shu cuttings, conventional method cuttage seedling raising method Piao Shu rooting of cuttings rates are only 46.3%, and using the method for the invention provided, rooting of cuttings rate is improved to more than 60.8, effect is notable.
Description
Technical field
The invention belongs to planting technology field, and in particular to a kind of Piao Shu cuttage seedling raising method.
Background technology
Piao Shu, Ulmaceae Celtis deciduous tree, bark is smooth, grey;Being born in Piao Shu at the resistance to shade in Plain, property light, thermophilic more
Warm moist climate, it is suitable to be born in fertile flat ground.Not tight is required to soil, there is certain Drought resistance, it is also water-fast wet and barren
Soil, adaptive faculty is stronger, is a kind of excellent Landscape Tree Species.
Piao Shuchang uses seminal propagation as breeding method, during using normal cutting propagation seedling method, Piao Shu cutting callus
Form difficult, cell differentiation speed is slow, causes rooting of cuttings rate relatively low, therefore relatively infrequently, but cuttage seedling raising method can be protected
Excellent maternal character is stayed, it is with the obvious advantage, therefore it is badly in need of a kind of Piao Shu cuttage seedling raising method, improve the rooting rate of cutting.
The content of the invention
In order to solve the above problems, the invention provides a kind of Piao Shu cuttage seedling raising method.
The present invention is achieved through the following technical solutions:
A kind of Piao Shu cuttage seedling raising method, specifically includes following steps:
(1)The screening of cutting
Annual mid or late April, preferably grow fine disease-free, the age of tree is maternal in 6-8 high-quality, select its 1-2 lifes wooden
Change or semi-lignified, diameter are in 1.5-2cm branch, tool chase is used at from taper 35-45cm, groove profile is U-shaped, groove depth
0.5cm or so, groove width 1.2-1.5cm, then smear ointment in notch, stimulate Callus formation, are grown while suppressing germ,
The ointment, its preparation method specifically includes following steps:
Extracting liquorice 8-10 parts by weight of A, 4-5 parts of Herba Andrographitis, 1-2 parts of the coptis clean drying, grind, add compound bacteria in
30-35 DEG C of fermentation process 30-35 minutes, then drying and sterilizing obtains medicinal powder;
B is by the medicinal powder, aloe ointment, sorbic acid in mass ratio 500:2000:1 it is well mixed after the ointment is made;
(2)The clip of cutting
After 10-15 days, the branch handled well is cut along U-type groove away from treetop side edge, notes avoiding callus in groove, then
Branch taper 5-10cm branch is clipped, length 18-22cm cutting is become, then using 0.1% sodium bicarbonate aqueous solution
U-type groove is rinsed well, can prevent ointment from remaining, current excessive should not prevent callus from coming off, then cutting blade is plucked
Remove, only retain 2-3 piece tender leafs, blade is then cut into half reduction transpiration, it is stand-by;Now method is obtained with callus
Cutting, the Callus formation time is faster and survival rate is high after cutting cuttage;
(3)The processing of cutting
End of the cutting with callus is used:80-90mg/L NAA solution soaks 2-3 hours, sterilization, cuttage;
(4)The cuttage of cutting
Cutting digs out nursery soil with scoop deep 5-6cm heatable adobe sleeping platform during cuttage, and cutting is carried to the end of callus
Downward 45 ° of cuttages are held, then fill compaction, it is to avoid hurt callus.
Beneficial effects of the present invention:Piao Shu branches are opened into U-type groove and ointment is smeared, help to stimulate Callus formation,
Suppress germ simultaneously to grow, it is ensured that callus is stable to be formed, the branch handled well is fabricated to along the cutting of U-type groove edge slotting
Bar, using cuttage after 80-90mg/L NAA solution immersion, stimulates Callus formation adventitious root, by experiment, uses this hair
A kind of Piao Shu of bright offer cuttage seedling raising method, substantially increases the rooting rate of Piao Shu cuttings, conventional method cuttage seedling raising method Piao
It is only 46.3% to set rooting of cuttings rate, and the method provided using the present invention, and rooting of cuttings rate is improved to more than 60.8, and effect shows
Write.
Embodiment
Embodiment 1
A kind of Piao Shu cuttage seedling raising method, specifically includes following steps:
(1)The screening of cutting
Annual mid or late April, preferably grow fine disease-free, the age of tree is maternal in 6-8 high-quality, select its 1-2 lifes wooden
Change or semi-lignified, diameter are in 1.5-2cm branch, tool chase is used at from taper 35-45cm, groove profile is U-shaped, groove depth
0.5cm or so, groove width 1.2-1.5cm, then smear ointment in notch, stimulate Callus formation, are grown while suppressing germ,
The ointment, its preparation method specifically includes following steps:
A 8 parts of extracting liquorice, 4 parts of Herba Andrographitis, 1 part of the coptis by weight clean drying, grind, add compound bacteria in 30 DEG C of hairs
Ferment is handled 30 minutes, and then drying and sterilizing obtains medicinal powder;
B is by the medicinal powder, aloe ointment, sorbic acid in mass ratio 500:2000:1 it is well mixed after the ointment is made;
(2)The clip of cutting
After 10 days, the branch handled well is cut along U-type groove away from treetop side edge, notes avoiding callus in groove, then cut
Branch taper 5cm branch is removed, length 18cm cutting is become, then using 0.1% sodium bicarbonate aqueous solution by U-type groove
Rinse well, can prevent ointment from remaining, current excessive should not prevent callus from coming off, then by cutting blade removal, only protect
2-3 piece tender leafs are stayed, blade is then cut into half reduction transpiration, it is stand-by;Now method obtains the cutting with callus, more
The injured tissue formation time is faster and survival rate is high after cutting cuttage;
(3)The processing of cutting
End of the cutting with callus is used:80-90mg/L NAA solution soaks 2-3 hours, sterilization, cuttage;
(4)The cuttage of cutting
Cutting digs out nursery soil with scoop deep 5-6cm heatable adobe sleeping platform during cuttage, and cutting is carried to the end of callus
Downward 45 ° of cuttages are held, then fill compaction, it is to avoid hurt callus.
Embodiment 2
A kind of Piao Shu cuttage seedling raising method, specifically includes following steps:
(1)The screening of cutting
Annual mid or late April, preferably grow fine disease-free, the age of tree is maternal in 6-8 high-quality, select its 1-2 lifes wooden
Change or semi-lignified, diameter are in 1.5-2cm branch, tool chase is used at from taper 35-45cm, groove profile is U-shaped, groove depth
0.5cm or so, groove width 1.2-1.5cm, then smear ointment in notch, stimulate Callus formation, are grown while suppressing germ,
The ointment, its preparation method specifically includes following steps:
9 parts of a extracting liquorices, 4.5 parts of Herba Andrographitis, 1.5 parts of the coptis clean drying, grind, and add compound bacteria at 34 DEG C of fermentations
Reason 33 minutes, then drying and sterilizing obtains medicinal powder;
B is by the medicinal powder, aloe ointment, sorbic acid in mass ratio 500:2000:1 it is well mixed after the ointment is made;
(2)The clip of cutting
After 12 days, the branch handled well is cut along U-type groove away from treetop side edge, notes avoiding callus in groove, then cut
Branch taper 5-10cm branch is removed, length 18-22cm cutting is become, then uses 0.1% sodium bicarbonate aqueous solution will
U-type groove is rinsed well, can prevent ointment from remaining, and current excessive should not prevent callus from coming off, then by cutting blade removal,
Only retain 2-3 piece tender leafs, blade is then cut into half reduction transpiration, it is stand-by;Now method obtains inserting with callus
Bar, the Callus formation time is faster and survival rate is high after cutting cuttage;
(3)The processing of cutting
End of the cutting with callus is used:80-90mg/L NAA solution soaks 2-3 hours, sterilization, cuttage;
(4)The cuttage of cutting
Cutting digs out nursery soil with scoop deep 5-6cm heatable adobe sleeping platform during cuttage, and cutting is carried to the end of callus
Downward 45 ° of cuttages are held, then fill compaction, it is to avoid hurt callus.
Comparative example 1
This comparative example 1 compared with Example 1, without step(1)The smearing ointment operation, method and step in addition
All same.
Comparative example 2
This comparative example 2 compared with Example 2, without step(4)The operation, using only normal cutting propagation method, except this
Outer method and step all same.
Control group
Conventional cuttage seedling raising method.
Piao Shu cuttage and seedling culture is carried out using upper 5 kinds of methods respectively, many experiments are broadcast, the rooting rate of cutting is counted, as a result
Such as table 1:
Table 1
Cutting number(Root) | Take root number(Root) | Rooting rate(%) | |
Embodiment 1 | 400 | 251 | 62.8 |
Embodiment 2 | 400 | 243 | 60.8 |
Comparative example 1 | 400 | 206 | 51.5 |
Comparative example 2 | 400 | 232 | 58.0 |
Control group | 400 | 185 | 46.3 |
As shown in Table 1, a kind of Piao Shu provided using the present invention cuttage seedling raising method, substantially increases taking root for Piao Shu cuttings
Rate, conventional method cuttage seedling raising method Piao Shu rooting of cuttings rates are only 46.3%, and the method provided using the present invention, rooting of cuttings
Rate is improved to more than 60.8%, and effect is notable.
Claims (1)
1. a kind of Piao Shu cuttage seedling raising method, it is characterised in that specifically include following steps:
(1)The screening of cutting
Annual mid or late April, preferably grow fine disease-free, the age of tree is maternal in 6-8 high-quality, select its 1-2 lifes wooden
Change or semi-lignified, diameter are in 1.5-2cm branch, tool chase is used at from taper 35-45cm, groove profile is U-shaped, groove depth
0.5cm or so, groove width 1.2-1.5cm, then smear ointment in notch, and the ointment, its preparation method specifically includes following step
Suddenly:
Extracting liquorice 8-10 parts by weight of A, 4-5 parts of Herba Andrographitis, 1-2 parts of the coptis clean drying, grind, add compound bacteria in
30-35 DEG C of fermentation process 30-35 minutes, then drying and sterilizing obtains medicinal powder;
B is by the medicinal powder, aloe ointment, sorbic acid in mass ratio 500:2000:1 it is well mixed after the ointment is made;
(2)The clip of cutting
After 10-15 days, the branch handled well is cut along U-type groove away from treetop side edge, callus in groove is avoided, then clip
Branch taper 5-10cm branch, becomes length 18-22cm cutting, and then use quality fraction is 0.1% sodium acid carbonate
The aqueous solution rinses U-type groove well, and current excessive should not prevent callus from coming off, then by cutting blade removal, only retain 2-3
Piece tender leaf, then cuts half reduction transpiration by blade, stand-by;
(3)The processing of cutting
End of the cutting with callus is used:80-90mg/L NAA solution soaks 2-3 hours, sterilization, cuttage;
(4)The cuttage of cutting
Cutting digs out nursery soil with scoop deep 5-6cm heatable adobe sleeping platform during cuttage, and cutting is carried to the end of callus
Downward 45 ° of cuttages are held, then fill compaction.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710227366.6A CN107114178A (en) | 2017-04-10 | 2017-04-10 | A kind of Piao Shu cuttage seedling raising method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710227366.6A CN107114178A (en) | 2017-04-10 | 2017-04-10 | A kind of Piao Shu cuttage seedling raising method |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107114178A true CN107114178A (en) | 2017-09-01 |
Family
ID=59725678
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710227366.6A Pending CN107114178A (en) | 2017-04-10 | 2017-04-10 | A kind of Piao Shu cuttage seedling raising method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107114178A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107580893A (en) * | 2017-09-26 | 2018-01-16 | 界首市东方果源家庭农场 | A kind of chrysanthemum cuttage seedling raising method for taking root fast |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102204489A (en) * | 2011-05-25 | 2011-10-05 | 广西壮族自治区林业科学研究院 | Method for culturing teak seedlings by spike culture and rapid propagation in container |
CN103518512A (en) * | 2013-10-17 | 2014-01-22 | 周家学 | Wild peach cutting seedling method |
CN104396487A (en) * | 2014-10-29 | 2015-03-11 | 陆川县大鹏现代农业发展有限公司 | Chinese mesona herb cutting planting method |
CN104663199A (en) * | 2015-02-02 | 2015-06-03 | 重庆绿带农业开发有限公司 | Colorful-osmanthus cottage method |
CN105165421A (en) * | 2015-10-23 | 2015-12-23 | 宜宾云辰乔木园林有限责任公司 | Top grafting method of golden silk willows |
CN105706838A (en) * | 2016-03-14 | 2016-06-29 | 山东省果树研究所 | Seedling breeding method of cherry rootstocks |
-
2017
- 2017-04-10 CN CN201710227366.6A patent/CN107114178A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102204489A (en) * | 2011-05-25 | 2011-10-05 | 广西壮族自治区林业科学研究院 | Method for culturing teak seedlings by spike culture and rapid propagation in container |
CN103518512A (en) * | 2013-10-17 | 2014-01-22 | 周家学 | Wild peach cutting seedling method |
CN104396487A (en) * | 2014-10-29 | 2015-03-11 | 陆川县大鹏现代农业发展有限公司 | Chinese mesona herb cutting planting method |
CN104663199A (en) * | 2015-02-02 | 2015-06-03 | 重庆绿带农业开发有限公司 | Colorful-osmanthus cottage method |
CN105165421A (en) * | 2015-10-23 | 2015-12-23 | 宜宾云辰乔木园林有限责任公司 | Top grafting method of golden silk willows |
CN105706838A (en) * | 2016-03-14 | 2016-06-29 | 山东省果树研究所 | Seedling breeding method of cherry rootstocks |
Non-Patent Citations (2)
Title |
---|
何国生: "《园林树木学》", 30 September 2008 * |
肖深根: "《中药材采种与种苗繁育》", 31 October 2012 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107580893A (en) * | 2017-09-26 | 2018-01-16 | 界首市东方果源家庭农场 | A kind of chrysanthemum cuttage seedling raising method for taking root fast |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103563745B (en) | A kind of method for tissue culture of ilex verticillata | |
CN104756867B (en) | The method for building up of camellia azalea Callus of Leaf regenerating system | |
Lee et al. | Improved cryopreservation of chrysanthemum (Chrysanthemum morifolium) using droplet-vitrification | |
CN104885768A (en) | Method for cuttage reproduction of horseradish tree | |
CN103609313B (en) | Pineapple leaf bud cuttage method | |
CN104620832A (en) | Cutting propagation method for ancient platycladus orientalis | |
CN104838868A (en) | Hydrangea cuttage reproduction method | |
CN104920077A (en) | High-survival-rate litchi tree grafting method | |
CN104542298A (en) | Grape tissue regeneration culture method | |
CN106613147A (en) | Cutting seedling raising method for pomegranates | |
CN109197203A (en) | A kind of vegetational zone leaf engrafting method | |
CN104255499B (en) | A kind of method of Radix seu Ramulus Diospyroris rhombifoliae tissue culture and rapid proliferation | |
CN110150147B (en) | Tissue culture and rapid propagation method for populus euphratica | |
CN107114178A (en) | A kind of Piao Shu cuttage seedling raising method | |
CN107094567A (en) | A kind of method of promotion cinnamomum camphora rooting of cuttings | |
CN106613993B (en) | A kind of cultural method of the tissue cultures regrowth of trifoliate orange | |
CN104904369B (en) | A kind of hair Lai seed accelerating germination methods | |
Hosseinpour et al. | High frequency in vitro propagation of M× M60, a cherry rootstock: the effects of culture media and growth regulators | |
CN104186181A (en) | Litsea glutinosa cuttage method | |
Vinothkumar et al. | Clonal propagation of Wattakaka volubilis through nodal explant culture | |
CN104067863A (en) | Walnut top grating method | |
KR101971978B1 (en) | Method for vegetative propagation of Chaenomeles sinensis using somatic embryo induction and plant regeneration | |
OKA et al. | HYPOCOTYL EXPLANTS | |
CN111972287A (en) | Tissue culture method for spanish burley grass | |
CN111296286A (en) | Moringa oleifera tetraploid induction and propagation method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170901 |
|
RJ01 | Rejection of invention patent application after publication |