CN104904369B - A kind of hair Lai seed accelerating germination methods - Google Patents
A kind of hair Lai seed accelerating germination methods Download PDFInfo
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- CN104904369B CN104904369B CN201510274709.5A CN201510274709A CN104904369B CN 104904369 B CN104904369 B CN 104904369B CN 201510274709 A CN201510274709 A CN 201510274709A CN 104904369 B CN104904369 B CN 104904369B
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Abstract
The invention provides a kind of hair Lai seed accelerating germination methods, including (1) seed collection；(2) seed pre-treatment；(3) acid etching；(4) gibberellin is handled；(5) germinate, produce.Relative to prior art, hair Lai seed accelerating germination methods of the present invention, a problem of mao Lai seeds deep dormancy can not only effectively be overcome, can not be germinateed then, and the method for combining alternating temperature round the clock by extending seed soaking time, a mao Lai percentages of seedgermination greatly improved in prior art basis.
Description
Technical field
The present invention relates to a kind of hair Lai seed accelerating germination methods, belong to a mao Lai reproduction techniques field.
Background technology
Hair Lai is Cornaceae deciduous tree, is born in the shaw of mountain valley, the country is distributed on the south Hebei, Shanxi and the Changjiang river
Each provinces and regions.Also known as bicycle beam wood, growth is extremely slow, and many decades lose it and increased, wooden as hard as iron, and axe difficulty is cut, and saws difficult disconnected.Pass
When saying that Confucius multiplies horse traction wood car and travelled through all the kingdoms then, jolting for Chang Yinlu and cause that bicycle beam is disconnected to ruin, after meet this wood by chance, cut down and do bicycle beam,
Though going through frustration, the distant horse in road is thin, and bicycle beam has no hurt unexpectedly.This tree is referred to as bicycle beam wood by descendant Gu Er.Dietotherapeutic is woody
Plant, its branches and leaves are used as medicine, browse and seed can food, it is among the people with a long history in China.Traditional oil tree, fruit contain
Oil is edible or make senior lubricant up to 27-38%, and the dregs of fat are capable of using as feed and fertilizer；Timber is hard, has a fine grain, be attractive in appearance,
Furniture, vehicle, farm implements etc. can be used as；Leaf and bark can obtain through refining mimosa extract, but also as " by the of four " greening and water and soil conservation seeds.
Hair Lai has obvious dormancy habit, and kind skin is hard, and undressed seed can hardly germinate then, even if through
Crossing Stratificated treatment and typically also taking 2-3 to germinate.At present, existing hair Lai Stratification techniques, existing hair Lai seeds be can solve the problem that
The problem of deep dormancy, slow germination, but germination percentage is only 60% or so, effect is not satisfactory.
The content of the invention
Goal of the invention：In order to overcome the deficiencies in the prior art, the invention provides a kind of hair Lai presprouting of seeds sides
Method.
Technical scheme：To achieve the above object, the present invention provides a kind of hair Lai seed accelerating germination methods, comprises the following steps：
(1) seed collection：It is acquired when the exocarp of hair Lai seeds is even completely black by green blackening；
(2) seed pre-treatment：The seed that step (1) gathers is dried, is then soaked in water, is carried out after exocarp softening
Manually rub to remove exocarp, then seed is cleaned and drifts out flat grain, dries, is placed in culture dish, it is standby；
(3) acid etching：By the seed obtained in step (2) and strong sulfuric acid response 1-15 minutes, clear water rinses 6-8 hours, then
Artificial rubbing removes the debris of acid etching, and water purification rinses 30-50 minutes, dried；
(4) gibberellin is handled：Seed obtained by step (3) is added in Gibberellins solution and soaked seed 80-100 days；
(5) germinate：Seed obtained by step (4) is taken out, germinates, produces in wet sand under normal temperature.
Preferably, step (2) reclaimed water is aqueous sodium carbonate, with weight ratio meter, concentration 6-8%.
As another kind preferably, soaking temperature is 30-40 degree in the step (2).
As another kind preferably, the concentration of the concentrated sulfuric acid is 98% in the step (3).
As another kind preferably, the concentration of gibberellin is 450-550mg/L in the step (4).
As another kind preferably, during step (4) seed soaking, daytime, keeping temperature was 18-22 DEG C, and night keeping temperature is
3-7℃。
Hair Lai seed accelerating germination methods provided by the invention, asked for existing hair Lai presproutings of seeds technology germination percentage is undesirable
Topic, pre-treatment and acid etching first are carried out to seed, and soaked seed with gibberellin, so as to effective breaking dormancy.Most importantly exist
During seed soaking, using method of the seed soaking time with reference to alternating temperature round the clock is extended, percentage of seedgermination effectively greatly improved.
Beneficial effect：Relative to prior art, hair Lai seed accelerating germination methods of the present invention, a mao Lai kinds can not only be effectively overcome
Sub- deep dormancy, the problem of can not germinateing then, and the method for combining alternating temperature round the clock by extending seed soaking time, in prior art
On the basis of a mao Lai percentages of seedgermination greatly improved.
Embodiment
The present invention is further described with reference to embodiment.
Embodiment 1
(1) seed collection：It is acquired when the exocarp of hair Lai seeds is even completely black by green blackening；
(2) seed pre-treatment：The seed that step (1) gathers is dried, then soaked with aqueous sodium carbonate, with weight ratio
Meter, concentration of sodium carbonate 6%, soaking temperature are 30 degree, after exocarp softening manually rub to remove exocarp, then
Seed is cleaned and drifts out flat grain, dries, is placed in culture dish, it is standby；
(3) acid etching：By strong sulfuric acid response that the seed obtained in step (2) and concentration are 98% 1 minute, clear water rinsed 6
Hour, then the debris of artificial rubbing removal acid etching, water purification flushing 30 minutes, dry；
(4) gibberellin is handled：Seed obtained by step (3) is added in the Gibberellins solution that concentration is 450mg/L and soaked seed
100 days, daytime, keeping temperature was 18 DEG C, and night keeping temperature is 3 DEG C；
(5) germinate：Seed obtained by step (4) is taken out, germinates, produces in wet sand under normal temperature.
Embodiment 2
(1) seed collection：It is acquired when the exocarp of hair Lai seeds is even completely black by green blackening；
(2) seed pre-treatment：The seed that step (1) gathers is dried, then soaked with aqueous sodium carbonate, with weight ratio
Meter, concentration of sodium carbonate 8%, soaking temperature are 40 degree, after exocarp softening manually rub to remove exocarp, then
Seed is cleaned and drifts out flat grain, dries, is placed in culture dish, it is standby；
(3) acid etching：By strong sulfuric acid response that the seed obtained in step (2) and concentration are 98% 15 minutes, clear water rinsed 8
Hour, then the debris of artificial rubbing removal acid etching, water purification flushing 50 minutes, dry；
(4) gibberellin is handled：Seed obtained by step (3) is added in the Gibberellins solution that concentration is 550mg/L and soaked seed
80 days, daytime, keeping temperature was 22 DEG C, and night keeping temperature is 7 DEG C；
(5) germinate：Seed obtained by step (4) is taken out, germinates, produces in wet sand under normal temperature.
Embodiment 3 (without intermittent warming round the clock during seed soaking)
(1) seed collection：It is acquired when the exocarp of hair Lai seeds is even completely black by green blackening；
(2) seed pre-treatment：The seed that step (1) gathers is dried, then soaked with aqueous sodium carbonate, with weight ratio
Meter, concentration of sodium carbonate 7%, soaking temperature are 35 degree, after exocarp softening manually rub to remove exocarp, then
Seed is cleaned and drifts out flat grain, dries, is placed in culture dish, it is standby；
(3) acid etching：By strong sulfuric acid response that the seed obtained in step (2) and concentration are 98% 8 minutes, clear water rinsed 7
Hour, then the debris of artificial rubbing removal acid etching, water purification flushing 40 minutes, dry；
(4) gibberellin is handled：Seed obtained by step (3) is added in the Gibberellins solution that concentration is 500mg/L and soaked seed
90 days；
(5) germinate：Seed obtained by step (4) is taken out, germinates, produces in wet sand under normal temperature.
Embodiment 4
(1) seed collection：It is acquired when the exocarp of hair Lai seeds is even completely black by green blackening；
(2) seed pre-treatment：The seed that step (1) gathers is dried, then soaked with aqueous sodium carbonate, with weight ratio
Meter, concentration of sodium carbonate 7%, soaking temperature are 35 degree, after exocarp softening manually rub to remove exocarp, then
Seed is cleaned and drifts out flat grain, dries, is placed in culture dish, it is standby；
(3) acid etching：By strong sulfuric acid response that the seed obtained in step (2) and concentration are 98% 8 minutes, clear water rinsed 7
Hour, then the debris of artificial rubbing removal acid etching, water purification flushing 40 minutes, dry；
(4) gibberellin is handled：Seed obtained by step (3) is added in the Gibberellins solution that concentration is 500mg/L and soaked seed
90 days, daytime, keeping temperature was 20 DEG C, and night keeping temperature is 5 DEG C；
(5) germinate：Seed obtained by step (4) is taken out, germinates, produces in wet sand under normal temperature.
Embodiment 5
(1) seed collection：It is acquired when the exocarp of hair Lai seeds is even completely black by green blackening；
(2) seed pre-treatment：The seed that step (1) gathers is dried, then soaked with aqueous sodium carbonate, with weight ratio
Meter, concentration of sodium carbonate 7%, soaking temperature are 32 degree, after exocarp softening manually rub to remove exocarp, then
Seed is cleaned and drifts out flat grain, dries, is placed in culture dish, it is standby；
(3) acid etching：By strong sulfuric acid response that the seed obtained in step (2) and concentration are 98% 4 minutes, clear water rinsed 7
Hour, then the debris of artificial rubbing removal acid etching, water purification flushing 35 minutes, dry；
(4) gibberellin is handled：Seed obtained by step (3) is added in the Gibberellins solution that concentration is 480mg/L and soaked seed
95 days, daytime, keeping temperature was 19 DEG C, and night keeping temperature is 4 DEG C；
(5) germinate：Seed obtained by step (4) is taken out, germinates, produces in wet sand under normal temperature.
Embodiment 6
(1) seed collection：It is acquired when the exocarp of hair Lai seeds is even completely black by green blackening；
(2) seed pre-treatment：The seed that step (1) gathers is dried, then soaked with aqueous sodium carbonate, with weight ratio
Meter, concentration of sodium carbonate 7%, soaking temperature are 38 degree, after exocarp softening manually rub to remove exocarp, then
Seed is cleaned and drifts out flat grain, dries, is placed in culture dish, it is standby；
(3) acid etching：By strong sulfuric acid response that the seed obtained in step (2) and concentration are 98% 12 minutes, clear water rinsed 7
Hour, then the debris of artificial rubbing removal acid etching, water purification flushing 45 minutes, dry；
(4) gibberellin is handled：Seed obtained by step (3) is added in the Gibberellins solution that concentration is 520mg/L and soaked seed
85 days, daytime, keeping temperature was 21 DEG C, and night keeping temperature is 6 DEG C；
(5) germinate：Seed obtained by step (4) is taken out, germinates, produces in wet sand under normal temperature.
Experimental example hair Lai seed accelerating germination methods germination percentage of the present invention is investigated
Gather with a batch of mao of Lai seed, carry out vernalization according to the embodiment of the present invention 3,4,5 and 6 germination accelerating methods respectively,
The quantity for terminating rear selected seed in step (2) is 100, carries out subsequent treatment；
According to the germination accelerating method of the embodiment of the present invention 3, only change seed soaking time therein as 2 hours, as a control group；
Each group germination percentage investigates result and see the table below 1
The each group germination accelerating method hair Lai germination percentage results of table 1
Note：Compared with control group, * P<0.05；Compared with 3 groups of embodiment, #P<0.05.
It can be obtained by the result of upper table 1, compared with control group, by the embodiment of the present invention 3, embodiment 4, embodiment 5 and implemented
After the germination accelerating method vernalization of example 6, the germination percentage of hair Lai seeds is significantly increased.
In addition, compared with 3 groups of embodiment, i.e., compared with method when seed soaking without intermittent warming round the clock, the present invention is real
After applying example 4, embodiment 5 and the method vernalization of embodiment 6, the germination percentage of hair Lai seeds is significantly increased, and can reach 87%.Table
It is bright to combine intermittent warming process round the clock by extending seed soaking time, mao Lai seeds can be greatly improved in prior art basis
Germination percentage, effect are preferable.
Claims (1)
1. a kind of mao Lai seed accelerating germination methods, it is characterised in that comprise the following steps：
(1) seed collection：It is acquired when the exocarp of hair Lai seeds is even completely black by green blackening；
(2) seed pre-treatment：The seed that step (1) gathers is dried, then with the sodium carbonate that concentration is 6-8% (weight ratio)
Solution soaks, and soaking temperature is 30-40 degree, after exocarp softening manually rub to remove exocarp, then by seed
Clean and drift out flat grain, dry, be placed in culture dish, it is standby；
(3) acid etching：By the seed obtained in step (2) and the strong sulfuric acid response 1-15 minutes that concentration is 98%, clear water rinses 6-8
Hour, then the debris of artificial rubbing removal acid etching, water purification flushing 30-50 minutes, dry；
(4) gibberellin is handled：Seed obtained by step (3) is added to concentration as the 80- that soaked seed in 450-550mg/L Gibberellins solutions
100 days, daytime, keeping temperature was 18-22 DEG C, and night keeping temperature is 3-7 DEG C；
(5) germinate：Seed obtained by step (4) is taken out, germinates, produces in wet sand under normal temperature.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510274709.5A CN104904369B (en) | 2015-05-26 | 2015-05-26 | A kind of hair Lai seed accelerating germination methods |
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| CN201510274709.5A CN104904369B (en) | 2015-05-26 | 2015-05-26 | A kind of hair Lai seed accelerating germination methods |
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| CN104904369A CN104904369A (en) | 2015-09-16 |
| CN104904369B true CN104904369B (en) | 2017-12-05 |
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| CN105409678B (en) * | 2015-11-20 | 2017-12-29 | 山东万路达园林科技有限公司 | A kind of hair Lai seed scale vernalization and method for culturing seedlings |
| CN105284406B (en) * | 2015-11-30 | 2018-08-14 | 金陵科技学院 | A kind of seed simple germination method of mao of Lai seed |
| CN106612709A (en) * | 2016-10-13 | 2017-05-10 | 合肥润雨农业科技有限公司 | Hackberry seed germination accelerating method |
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