Snow red cuttage breeding method
Technical field
The invention belongs to the vegetative propagation technique field of plant, and in particular to snow red cuttage breeding method.
Background technology
Snow red(Ardisia villosaRoxb), alias medicine teacher, the frizzle Japanese ardisia, it is Myrsinacea Ardisa
Evergreen dwarf shrub, high 0.5 ~ 1m is dilute up to 2 ~ 3m, has creeping rootstock.When young almost complete stool by taupe or the long pubescence of rust or length
Bristle, hair often crimp, after it is gradually hairless.Blade heavily fortified point papery, ellipticity lanceolar is to avette;Single or multiple poly- umbel or umbrella shape flower
Sequence, by the long pubescence of rust, side is raw or is born in the side special spray top of life;Spray grows 2 ~ 15cm, and the nearly top of elder often has 1 ~ 2
Leaf or degeneration leaf;Bennet grows 5 ~ 10mm;Spend long 5 ~ 8mm;Petal lavender or pink, dilute white, avette to wide lanceolar;Fruit
Spherical, 5 ~ 7mm of diameter, peony or with black has gland point, hair.5 ~ July of florescence, fruiting period 2 ~ May of next year.China mainly divides
The provinces and regions such as Yunnan, Guangxi, Guangdong are distributed in, Vietnam to Indian peninsula east also has, and is born under the thin of 500 ~ 1540m of height above sea level, thick forest
Between crack of stone, slope side or roadside Yang Chu, also see and cover moist place.Snow red complete stool hyoscine, there is detumescence, promoting blood circulation to remove blood stasis
Effect, for treating rheumatic ostealgia, traumatic injury, haematemesis, red dysentery characterized by white mucous stool, sore scabies etc..Snow red flower, leaf and fruit all has higher sight
Reward value, and it is resistance to shady, water-fast wet, it is afforestation potentiality kind.About snowing, there has been no document report for red mating system.
The content of the invention
It is an object of the invention to provide one kind can keep maternal merit, and survival rate of plant is high, reproduction speed is fast,
The red cuttage breeding method that snows of the easy and easy popularization and application of cuttage technique.
To reach above-mentioned purpose, the present invention uses following technical scheme:
Snow red cuttage breeding method, comprises the following steps:
(1)Matrix prepares:Matrix is peat soil, vermiculite, one kind in river sand, or is peat soil and river sand, Huo Zhewei
Peat soil, vermiculite and perlite, matrix is puddled uniformly, and matrix is sprayed with liquor potassic permanganate, using irrigate matrix as
Standard, use is opened after sealing 2 ~ 3d with plastic sheeting;
(2)Adopt fringe:Using red 3 years raw plant of snowing as female parent, using healthy and strong, disease-free semi-lignified edible tender branch, slightly
Spend for 0.5 ~ 0.8cm, with adopt with;
(3)Cuttings prepares:Fringe bar is cut into 7 ~ 10cm short fringe, retains 2 ~ 3 internodes, cuttings top clip is truncate, under
30 ~ 45 ° of portion's clip, away from 0.8 ~ 1.2cm of section, cuttings top retains 2 ~ 3 leaves, the cuttings trimmed is put into moisturizing in clear water;
(4)Root-growing agent processing:Cuttings is taken out, after cuttings base portion moisture is slightly dry, quickly dipped 4 ~ 6s in root-growing agent;
(5)Cuttage:It is in 45 ° of oblique angles by cuttings, is drawn with thin waddy and be inserted into matrix, tamp interpolation slit conveniently, cuttage depth is
The 1/3 ~ 1/2 of cutting length, sprays water immediately after cuttage, to ensure that cuttings bar fully contacts with matrix;
(6)Managed after inserting:Watered using artificial, during cuttage root-taking, keep relative air humidity 60 ~ 80%, air themperature
25 ~ 28 DEG C, water content of substrate 60 ~ 80%, 6000 ~ 8000LX of intensity of illumination;The next morning or evening sprinkling bactericidal liquid after cuttage
Once, every 7 ~ 8d sprays 1 bactericidal liquid to cuttings afterwards, until cuttings stops sprinkling after all taking root;After cuttage 20d it
It is interior, potassium dihydrogen phosphate is sprayed weekly once;
(7)Transplanting:Start to take root after 20 ~ 25d of cuttings cuttage, spray potassium dihydrogen phosphate once after taking root, and reduce water spray
Number, pot transplanting can be gone up after 35d.
Further, the step(1)The concentration of middle liquor potassic permanganate is 0.5 ~ 1wt%.
Further, the step(1)The volume ratio of middle peat soil and river sand is 1:1.
Further, the step(1)The volume ratio of middle peat soil, vermiculite and perlite is 5:3:2.
Further, the step(1)Mesostroma thickness is 8 ~ 10cm.
Further, the step(4)The concentration of middle root-growing agent is 0 ~ 500mg/L.
Further, the step(4)Middle root-growing agent is at least one of methyl α-naphthyl acetate, heteroauxin, indolebutyric acid.
Further, the step(5)Middle cuttage spacing in the rows is 8 ~ 12cm, and line-spacing is 8 ~ 12cm.
Further, the step(6)Middle bactericidal liquid is 800 ~ 1000 times of liquid of carbendazim.
Further, the step(6)The concentration of middle potassium dihydrogen phosphate is 0.05 ~ 0.2wt%.
Further, the step(7)The concentration of middle potassium dihydrogen phosphate is 0.5 ~ 2wt%.
The invention has the advantages that:
The cottage method of the present invention can keep the red maternal merit that snows, and survival rate of plant is high, passes through postgraduate
After the species and concentration of root agent, the species of matrix, in the most preferred embodiment, rooting rate is transplanted into up to more than 85%
Motility rate can reach 95%;Cutting propagation speed is fast, and plant strain growth is neat, healthy and strong, and the easy easy popularization and application of cuttage technique program,
Production efficiency is substantially increased, the demand of afforestation and medical market can be better met.
Embodiment
With reference to specific embodiment, the present invention is described further:
Embodiment 1
Follow the steps below the red cutting propagation that snows:
(1)Matrix prepares:Matrix is using peat soil, river sand(Volume ratio 1:1), matrix is puddled uniformly, stromal thickness is
9cm or so, matrix is sprayed with 0.5wt% liquor potassic permanganate, be defined by irrigating matrix, 3d is sealed with plastic sheeting
After open use;
(2)Adopt fringe:In the morning, it is tender using healthy and strong, disease-free semi-lignified children using red 3 years raw plant of snowing as female parent
Branch, rugosity are 0.5 ~ 0.8cm, with adopt with;
(3)Cuttings prepares:Fringe bar is cut into 7 ~ 10cm short fringe, retains 2 ~ 3 internodes, cuttings top clip is truncate, under
30 ~ 45 ° of portion's clip, away from section 1cm or so, cuttings top retains 2 ~ 3 leaves, and the cuttings trimmed is put into moisturizing in clear water;
(4)Root-growing agent processing:Cuttings is taken out, after cuttings base portion moisture is slightly dry, quickly dipped 5s, root-growing agent in root-growing agent
For 100mg/L methyl α-naphthyl acetate;
(5)Cuttage:It is in 45 ° of oblique angles by cuttings, is drawn with thin waddy and be inserted into matrix, tamp interpolation slit conveniently, cuttage depth is
The 1/3 ~ 1/2 of cutting length, cuttage spacing in the rows are 10cm, and line-spacing 10cm, 90 plants of cuttage, spray water immediately after cuttage altogether, to protect
Card cuttings bar fully contacts with matrix;
(6)Managed after inserting:Watered using artificial, during cuttage root-taking, keep relative air humidity 60 ~ 80%, air themperature
25 ~ 28 DEG C, water content of substrate 60 ~ 80%, 6000 ~ 8000LX of intensity of illumination;To prevent cuttings infection morbidity, second day after cuttage
Spray 800 times of liquid of carbendazim once in the morning, sprays 1 carbendazim, 800 times of liquid to cuttings per 7d afterwards, until cuttings is all taken root
Stop sprinkling afterwards;After cuttage within 20d, 0.1wt% potassium dihydrogen phosphates are sprayed weekly once, promote cuttage rooting;
(7)Transplanting:Start to take root after 20 ~ 25d of cuttings cuttage, spray 1wt% potassium dihydrogen phosphates once after taking root, promote root
It is lignifying, and reduces water spray number, pot transplanting can be gone up after 35d.
The change of 1 cuttings is observed after cuttage every 5d, 30 ~ 35d carries out data statistics to it, and observation index includes taking root
Rate, average adventitious root bar number, most long indefinite root long, average root long.
Statistical result is:The rooting rate of cuttings is 56.7%, and average adventitious root bar number 4.2, most long adventitious root is a length of
3.3cm, average root long 1.9cm.
Embodiment 2
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 300mg/L methyl α-naphthyl acetate.
Statistical result is:The rooting rate of cuttings is 86.7%, and average adventitious root bar number 4.9, most long adventitious root is a length of
4.5cm, average root long 2.6cm.
Embodiment 3
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 500mg/L methyl α-naphthyl acetate.
Statistical result is:The rooting rate of cuttings is 36.7%, and average adventitious root bar number 4.8, most long adventitious root is a length of
3.8cm, average root long 1.8cm.
Embodiment 4
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 100mg/L indolebutyric acid.
Statistical result is:The rooting rate of cuttings is 33.3%, and average adventitious root bar number 2.2, most long adventitious root is a length of
3.5cm, average root long 1.6cm.
Embodiment 5
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 300mg/L indolebutyric acid.
Statistical result is:The rooting rate of cuttings is 80.0%, and average adventitious root bar number 3.9, most long adventitious root is a length of
3.5cm, average root long 2.0cm.
Embodiment 6
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 500mg/L indolebutyric acid.
Statistical result is:The rooting rate of cuttings is 56.7%, and average adventitious root bar number 3.4, most long adventitious root is a length of
3.8cm, average root long 2.5cm.
Embodiment 7
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 100mg/L heteroauxin.
Statistical result is:The rooting rate of cuttings is 56.7%, and average adventitious root bar number 3.9, most long adventitious root is a length of
4.5cm, average root long 1.7cm.
Embodiment 8
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 300mg/L heteroauxin.
Statistical result is:The rooting rate of cuttings is 66.7%, and average adventitious root bar number 4.0, most long adventitious root is a length of
4.0cm, average root long 2.2cm.
Embodiment 9
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 500mg/L heteroauxin.
Statistical result is:The rooting rate of cuttings is 66.7%, and average adventitious root bar number 3.3, most long adventitious root is a length of
3.8cm, average root long 2.1cm.
Embodiment 10
The red cutting propagation that snows is carried out according to the step of embodiment 1, without any root-growing agent, the quickly dipped clear water of cuttings base portion
5s。
Statistical result is:The rooting rate of cuttings is 60.0%, and average adventitious root bar number 4.0, most long adventitious root is a length of
3.8cm, average root long 2.2cm.
From case study on implementation 1 ~ 10, the cuttings handled with the 3 kinds of root-growing agents or clear water of various concentrations can be successful, raw
The species and concentration of root agent have an impact to rooting rate, wherein, the rooting percent highest of 300mg/L methyl α-naphthyl acetate processing, reach
86.7%, and its average adventitious root bar number, average root long all showed in all processing it is optimal.
Embodiment 11
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 300mg/L indolebutyric acid, and matrix is adopted
Use peat soil.
Statistical result is:The rooting rate of cuttings is 50.0%, and average adventitious root bar number 2.6, most long adventitious root is a length of
3.5cm, average root long 1.8cm.
Embodiment 12
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 300mg/L indolebutyric acid, and matrix is adopted
Use vermiculite.
Statistical result is:The rooting rate of cuttings is 56.7%, and average adventitious root bar number 4.5, most long adventitious root is a length of
3.2cm, average root long 1.9cm.
Embodiment 13
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 300mg/L indolebutyric acid, and matrix is adopted
Use river sand.
Statistical result is:The rooting rate of cuttings is 43.3%, and average adventitious root bar number 3.5, most long adventitious root is a length of
2.5cm, average root long 1.3cm.
Embodiment 14
The red cutting propagation that snows is carried out according to the step of embodiment 1, root-growing agent is 300mg/L indolebutyric acid, and matrix is adopted
With peat soil, vermiculite, perlite(Volume ratio 5:3:2).
Statistical result is:The rooting rate of cuttings is 70.0%, and average adventitious root bar number 4.7, most long adventitious root is a length of
4.5cm, average root long 2.1cm.
, can be successful using different substrates cuttings from case study on implementation 1,11 ~ 14, wherein using volume ratio as 5:3:2
When peat soil, vermiculite, perlite are as matrix, rooting rate, average adventitious root bar number, most long indefinite root long, average root long etc. refer to
Mark shows optimal.
Embodiment 15
Follow the steps below the red cutting propagation that snows:
(1)Matrix prepares:Matrix is using peat soil, vermiculite, perlite(Volume ratio 5:3:2), matrix is puddled uniformly, base
Matter thickness is 8cm, and matrix is sprayed with 1wt% liquor potassic permanganate, is defined by irrigating matrix, is sealed with plastic sheeting
Use is opened after 2d;
(2)Adopt fringe:It is tender using healthy and strong, disease-free semi-lignified children using red 3 years raw plant of snowing as female parent at the cloudy day
Branch, rugosity are 0.5 ~ 0.8cm, with adopt with;
(3)Cuttings prepares:Fringe bar is cut into 7 ~ 10cm short fringe, retains 2 ~ 3 internodes, cuttings top clip is truncate, under
30 ~ 45 ° of portion's clip, away from section 1.0cm or so, cuttings top retains 2 ~ 3 leaves, and the cuttings trimmed is put into moisturizing in clear water;
(4)Root-growing agent processing:Cuttings is taken out, after cuttings base portion moisture is slightly dry, quickly dipped 4s, root-growing agent in root-growing agent
For 300mg/L indolebutyric acid;
(5)Cuttage:It is in 45 ° of oblique angles by cuttings, is drawn with thin waddy and be inserted into matrix, tamp interpolation slit conveniently, cuttage depth is
The 1/3 ~ 1/2 of cutting length, cuttage spacing in the rows are 8cm, line-spacing 8cm, are sprayed water immediately after cuttage, to ensure that cuttings bar and matrix are filled
Tap is touched;
(6)Managed after inserting:Watered using artificial, during cuttage root-taking, keep relative air humidity 60 ~ 80%, air themperature
25 ~ 28 DEG C, water content of substrate 60 ~ 80%, 6000 ~ 8000LX of intensity of illumination;To prevent cuttings infection morbidity, second day after cuttage
1000 times of liquid of sprinkling carbendazim once, spray 1 carbendazim, 1000 times of liquid per 8d to cuttings afterwards at night, until cuttings is all raw
Stop sprinkling after root;After cuttage within 20d, 0.05wt% potassium dihydrogen phosphates are sprayed weekly once, promote cuttage rooting;
(7)Transplanting:Start to take root after 20 ~ 25d of cuttings cuttage, spray 0.5wt% potassium dihydrogen phosphates once after taking root, promote
Root system lignifying, and water spray number is reduced, it can go up pot transplanting after 35d;Transplanting survival rate is 95%.
Embodiment 16
Follow the steps below the red cutting propagation that snows:
(1)Matrix prepares:Matrix is using peat soil, vermiculite, perlite(Volume ratio 5:3:2), matrix is puddled uniformly, base
Matter thickness is 10cm, and matrix is sprayed with 0.5wt% liquor potassic permanganate, is defined by irrigating matrix, close with plastic sheeting
Use is opened after envelope 3d;
(2)Adopt fringe:In the morning, it is tender using healthy and strong, disease-free semi-lignified children using red 3 years raw plant of snowing as female parent
Branch, rugosity are 0.5 ~ 0.8cm, with adopt with;
(3)Cuttings prepares:Fringe bar is cut into 7 ~ 10cm short fringe, retains 2 ~ 3 internodes, cuttings top clip is truncate, under
30 ~ 45 ° of portion's clip, away from 0.8 ~ 1.2cm of section, cuttings top retains 2 ~ 3 leaves, the cuttings trimmed is put into moisturizing in clear water;
(4)Root-growing agent processing:Cuttings is taken out, after cuttings base portion moisture is slightly dry, quickly dipped 6s, root-growing agent in root-growing agent
For 300mg/L indolebutyric acid;
(5)Cuttage:It is in 45 ° of oblique angles by cuttings, is drawn with thin waddy and be inserted into matrix, tamp interpolation slit conveniently, cuttage depth is
The 1/3 ~ 1/2 of cutting length, cuttage spacing in the rows are 12cm, line-spacing 12cm, are sprayed water immediately after cuttage, to ensure cuttings bar and matrix
Fully contact;
(6)Managed after inserting:Watered using artificial, during cuttage root-taking, keep relative air humidity 60 ~ 80%, air themperature
25 ~ 28 DEG C, water content of substrate 60 ~ 80%, 6000 ~ 8000LX of intensity of illumination;To prevent cuttings infection morbidity, second day after cuttage
800 times of liquid of sprinkling carbendazim once, spray 1 carbendazim, 800 times of liquid per 7d to cuttings afterwards at night, until cuttings is all taken root
Stop sprinkling afterwards;After cuttage within 20d, 0.2wt% potassium dihydrogen phosphates are sprayed weekly once, promote cuttage rooting;
(7)Transplanting:Start to take root after 20 ~ 25d of cuttings cuttage, spray 2wt% potassium dihydrogen phosphates once after taking root, promote root
It is lignifying, and reduces water spray number, pot transplanting can be gone up after 35d;Transplanting survival rate is 96%.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Belong to those skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in, all should
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be defined by scope of the claims.