Summary of the invention
The present invention, in order to make up the deficiencies in the prior art, provides a kind of one side pin cuttage and seedling culture method, and the Clonal regeneration that can be applicable to one side pin is produced.
To achieve these goals, technical scheme of the present invention is as follows:
1. an one side pin cuttage and seedling culture method, comprises the following steps:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with the cutting of bactericide immersion treatment;
(3) with the cutting of plant hormone immersion treatment;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, inserts 1 for every glass;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) opened film spraying fungicide every 7 days and prevent mould from growing after inserting, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock afterwards until go out garden and transplant.
The bactericide of the above step (2) is the 0.5% carbendazim aqueous solution.
The immersion treatment time of the above step (2) is 20 minutes, has soaked rear water purification and has rinsed.
The plant hormone of the above step (3) is the hormotone aqueous solution that indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) are mixedly configured into by a certain percentage, its compound method first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times.
The immersion treatment method of the above step (3) is that long for cutting lower end 1/3 part is soaked 1 hour.
The seedbed of the above step (4) is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand.
The matrix of the nutrition cup of the above step (4) is mixed with 2 ~ 3 parts of peats composed of rotten mosses, 2 parts of red heart soil and 1 ~ 2 part of coconut palm chaff that becomes thoroughly decomposed to form.
Compared with prior art, its beneficial effect is in the present invention:
One side pin cuttage and seedling culture method of the present invention, draw materials conveniently, operation is simple, and rooting rate reaches 80% ~ 90%, cutting medium is mixing Light media, and permeability is good, and the amount of taking root is large, and root system is healthy and strong flourishing, be beneficial to raising transplanting survival rate, cuttage and seedling culture can keep maternal good characteristic, and medicinal material drug effect is relatively stable.
Embodiment
Below in conjunction with concrete example of implementing, technical solution of the present invention is described in detail.
embodiment 1
A kind of one side pin cuttage and seedling culture method, its concrete steps are as follows:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with 0.5% carbendazim aqueous solution soaking process cutting 20 minutes, soaked rear water purification and rinsed;
(3) long 1/3 part in plant hormone aqueous solution soaking process cutting lower end 1 hour is used, the collocation method of the plant hormone aqueous solution got ready in advance first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, insert 1 for every glass, set seedbed is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand, nutrition cup is neatly put on furrow face, and nutrition cup matrix 2 parts of peats composed of rotten mosses, 2 parts of red heart soil and 1 part of coconut palm chaff that becomes thoroughly decomposed are mixed and form;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) to open film after inserting to spray the 0.5% carbendazim aqueous solution prevent mould from growing every 7 days, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock according to a conventional method afterwards.
Insert investigation in latter 60 days, this cuttage and seedling culture rooting of cuttings rate reaches 83.3%.
embodiment 2
A kind of one side pin cuttage and seedling culture method, its concrete steps are as follows:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with 0.5% carbendazim aqueous solution soaking process cutting 20 minutes, soaked rear water purification and rinsed;
(3) long 1/3 part in plant hormone aqueous solution soaking process cutting lower end 1 hour is used, the collocation method of the plant hormone aqueous solution got ready in advance first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, insert 1 for every glass, set seedbed is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand, nutrition cup is neatly put on furrow face, and nutrition cup matrix 3 parts of peats composed of rotten mosses, 2 parts of red heart soil and 1 part of coconut palm chaff that becomes thoroughly decomposed are mixed and form;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) to open film after inserting to spray the 0.5% carbendazim aqueous solution prevent mould from growing every 7 days, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock according to a conventional method afterwards.
Insert investigation in latter 60 days, this cuttage and seedling culture rooting of cuttings rate reaches 91.5%.
embodiment 3
A kind of one side pin cuttage and seedling culture method, its concrete steps are as follows:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with 0.5% carbendazim aqueous solution soaking process cutting 20 minutes, soaked rear water purification and rinsed;
(3) long 1/3 part in plant hormone aqueous solution soaking process cutting lower end 1 hour is used, the collocation method of the plant hormone aqueous solution got ready in advance first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, insert 1 for every glass, set seedbed is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand, nutrition cup is neatly put on furrow face, and nutrition cup matrix 2 parts of peats composed of rotten mosses, 2 parts of red heart soil and 2 parts of coconut palm chaffs that become thoroughly decomposed are mixed and form;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) to open film after inserting to spray the 0.5% carbendazim aqueous solution prevent mould from growing every 7 days, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock according to a conventional method afterwards.
Insert investigation in latter 60 days, this cuttage and seedling culture rooting of cuttings rate reaches 82.4%.
embodiment 4
A kind of one side pin cuttage and seedling culture method, its concrete steps are as follows:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with 0.5% carbendazim aqueous solution soaking process cutting 20 minutes, soaked rear water purification and rinsed;
(3) long 1/3 part in plant hormone aqueous solution soaking process cutting lower end 1 hour is used, the collocation method of the plant hormone aqueous solution got ready in advance first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, insert 1 for every glass, set seedbed is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand, nutrition cup is neatly put on furrow face, and nutrition cup matrix 3 parts of peats composed of rotten mosses, 2 parts of red heart soil and 2 parts of coconut palm chaffs that become thoroughly decomposed are mixed and form;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) to open film after inserting to spray the 0.5% carbendazim aqueous solution prevent mould from growing every 7 days, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock according to a conventional method afterwards.
Insert investigation in latter 60 days, this cuttage and seedling culture rooting of cuttings rate reaches 86.6%.