CN104604512A - Cutting seedling method for zanthoxylum dissitum - Google Patents

Cutting seedling method for zanthoxylum dissitum Download PDF

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Publication number
CN104604512A
CN104604512A CN201510067365.0A CN201510067365A CN104604512A CN 104604512 A CN104604512 A CN 104604512A CN 201510067365 A CN201510067365 A CN 201510067365A CN 104604512 A CN104604512 A CN 104604512A
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Prior art keywords
cuttage
side pin
cutting
seedling culture
culture method
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CN201510067365.0A
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CN104604512B (en
Inventor
黄开顺
李开祥
黄剑
王平
孙吉康
陈金艳
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Central South University of Forestry and Technology
Guangxi Zhuang Autonomous Region Forestry Research Institute
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Guangxi Zhuang Autonomous Region Forestry Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Environmental & Geological Engineering (AREA)
  • Pest Control & Pesticides (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a cutting seedling method for zanthoxylum dissitum, and belongs to the technical field of cultivation of Chinese herbal medicines. The method comprises the specific steps of branch picking, pruning, disinfection of cuttings, hormone treatment for the cuttings, preparation of a seedbed and nutrition cups, cuttage, water spraying for moisture preservation, shading, later stage disinfection, mulch film removing and the like. The cutting seedling method has the advantages that materials are convenient to obtain, the process is simple, the rooting percentage reaches 80%-90%, a cutting medium is a mixed light medium, permeability is good, the root number of the cuttings is large, the root system is robust and developed, the survival rate of transplanting can be improved, good characters of the female parent can be maintained through cutting seedling, the efficacy of herbal medicines is relatively stable, and sufficient high-quality seedlings can be provided for large-area artificial cultivation.

Description

A kind of one side pin cuttage and seedling culture method
Technical field
The present invention relates to technical field of Chinese herbal medicine cultivation, disclose a kind of one side pin cuttage and seedling culture method especially.
Background technology
One side pin formal name used at school a species of small clam living in fresh water shell Chinese prickly ash ( zanthoxylum dissitumhemsl.), for Rutaceae (Rutaceae) Zanthoxylum ( zanthoxylum) plant, mainly be distributed in Guangxi, Yunnan, Guizhou, Hunan, Hubei, Chongqing, Sichuan etc. and economize (district, city), it is a kind of precious Chinese herbal medicine, its root, stem, fruit all can be used as medicine, there is effect of promoting blood circulation to remove blood stasis, reunion of fractured tendons and bones, regulating qi-flowing for relieving pain, control traumatic injury, sprain, fracture and the disease such as hernia pain, studies have found that the polysaccharide in one side pin pericarp also has certain antitumor action (Yang Lin, a species of small clam living in fresh water shell Chinese prickly ash pericarp anti-tumor activity of polysaccharides research [J]. traditional Chinese medicine journal, 2012,40(5): 34-37.).Therefore, the demand of one side pin traditional Chinese medicine is increasing.But the Regional Distribution of one side pin is quite limited, in the middle of the normal cheuch being sporadicly distributed in tor region, under field conditions (factors), one side pin ripening rate is low, and seed coat is hard, and resting stage is long, natural reseeding fertility is low, and in order to obtain economic interests, people excessively excavate wild one side pin resource, cause wild resource endangered.For meeting market demand, people have to develop one side pin artificial cultivation technique, but present people carry out the nursery of one side pin and generally utilize seed to carry out sexual cultivation, its hereditary feature can change by relative parent, have the defects such as effective component content fluctuation is large, drug effect reduction, therefore develop one side pin cuttage asexual grow seedling very urgent.
Summary of the invention
The present invention, in order to make up the deficiencies in the prior art, provides a kind of one side pin cuttage and seedling culture method, and the Clonal regeneration that can be applicable to one side pin is produced.
To achieve these goals, technical scheme of the present invention is as follows:
1. an one side pin cuttage and seedling culture method, comprises the following steps:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with the cutting of bactericide immersion treatment;
(3) with the cutting of plant hormone immersion treatment;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, inserts 1 for every glass;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) opened film spraying fungicide every 7 days and prevent mould from growing after inserting, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock afterwards until go out garden and transplant.
The bactericide of the above step (2) is the 0.5% carbendazim aqueous solution.
The immersion treatment time of the above step (2) is 20 minutes, has soaked rear water purification and has rinsed.
The plant hormone of the above step (3) is the hormotone aqueous solution that indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) are mixedly configured into by a certain percentage, its compound method first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times.
The immersion treatment method of the above step (3) is that long for cutting lower end 1/3 part is soaked 1 hour.
The seedbed of the above step (4) is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand.
The matrix of the nutrition cup of the above step (4) is mixed with 2 ~ 3 parts of peats composed of rotten mosses, 2 parts of red heart soil and 1 ~ 2 part of coconut palm chaff that becomes thoroughly decomposed to form.
Compared with prior art, its beneficial effect is in the present invention:
One side pin cuttage and seedling culture method of the present invention, draw materials conveniently, operation is simple, and rooting rate reaches 80% ~ 90%, cutting medium is mixing Light media, and permeability is good, and the amount of taking root is large, and root system is healthy and strong flourishing, be beneficial to raising transplanting survival rate, cuttage and seedling culture can keep maternal good characteristic, and medicinal material drug effect is relatively stable.
Embodiment
Below in conjunction with concrete example of implementing, technical solution of the present invention is described in detail.
embodiment 1
A kind of one side pin cuttage and seedling culture method, its concrete steps are as follows:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with 0.5% carbendazim aqueous solution soaking process cutting 20 minutes, soaked rear water purification and rinsed;
(3) long 1/3 part in plant hormone aqueous solution soaking process cutting lower end 1 hour is used, the collocation method of the plant hormone aqueous solution got ready in advance first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, insert 1 for every glass, set seedbed is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand, nutrition cup is neatly put on furrow face, and nutrition cup matrix 2 parts of peats composed of rotten mosses, 2 parts of red heart soil and 1 part of coconut palm chaff that becomes thoroughly decomposed are mixed and form;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) to open film after inserting to spray the 0.5% carbendazim aqueous solution prevent mould from growing every 7 days, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock according to a conventional method afterwards.
Insert investigation in latter 60 days, this cuttage and seedling culture rooting of cuttings rate reaches 83.3%.
embodiment 2
A kind of one side pin cuttage and seedling culture method, its concrete steps are as follows:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with 0.5% carbendazim aqueous solution soaking process cutting 20 minutes, soaked rear water purification and rinsed;
(3) long 1/3 part in plant hormone aqueous solution soaking process cutting lower end 1 hour is used, the collocation method of the plant hormone aqueous solution got ready in advance first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, insert 1 for every glass, set seedbed is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand, nutrition cup is neatly put on furrow face, and nutrition cup matrix 3 parts of peats composed of rotten mosses, 2 parts of red heart soil and 1 part of coconut palm chaff that becomes thoroughly decomposed are mixed and form;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) to open film after inserting to spray the 0.5% carbendazim aqueous solution prevent mould from growing every 7 days, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock according to a conventional method afterwards.
Insert investigation in latter 60 days, this cuttage and seedling culture rooting of cuttings rate reaches 91.5%.
embodiment 3
A kind of one side pin cuttage and seedling culture method, its concrete steps are as follows:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with 0.5% carbendazim aqueous solution soaking process cutting 20 minutes, soaked rear water purification and rinsed;
(3) long 1/3 part in plant hormone aqueous solution soaking process cutting lower end 1 hour is used, the collocation method of the plant hormone aqueous solution got ready in advance first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, insert 1 for every glass, set seedbed is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand, nutrition cup is neatly put on furrow face, and nutrition cup matrix 2 parts of peats composed of rotten mosses, 2 parts of red heart soil and 2 parts of coconut palm chaffs that become thoroughly decomposed are mixed and form;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) to open film after inserting to spray the 0.5% carbendazim aqueous solution prevent mould from growing every 7 days, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock according to a conventional method afterwards.
Insert investigation in latter 60 days, this cuttage and seedling culture rooting of cuttings rate reaches 82.4%.
embodiment 4
A kind of one side pin cuttage and seedling culture method, its concrete steps are as follows:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with 0.5% carbendazim aqueous solution soaking process cutting 20 minutes, soaked rear water purification and rinsed;
(3) long 1/3 part in plant hormone aqueous solution soaking process cutting lower end 1 hour is used, the collocation method of the plant hormone aqueous solution got ready in advance first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the insertion matrix degree of depth is 1/3 of cutting length, insert 1 for every glass, set seedbed is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand, nutrition cup is neatly put on furrow face, and nutrition cup matrix 3 parts of peats composed of rotten mosses, 2 parts of red heart soil and 2 parts of coconut palm chaffs that become thoroughly decomposed are mixed and form;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) to open film after inserting to spray the 0.5% carbendazim aqueous solution prevent mould from growing every 7 days, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock according to a conventional method afterwards.
Insert investigation in latter 60 days, this cuttage and seedling culture rooting of cuttings rate reaches 86.6%.

Claims (8)

1. an one side pin cuttage and seedling culture method, is characterized in that comprising the following steps:
(1) gather annual stalwartness, one side pin spray without damage by disease and insect, skin zone's green, wipe out long winglike compound leaf, be cut into long 15cm, cutting with full bud point;
(2) with the cutting of bactericide immersion treatment;
(3) with the cutting of plant hormone immersion treatment;
(4) fill in the nutrition cup of matrix by cutting cuttage to seedbed, the degree of depth inserting matrix is 1/3 of cutting length, inserts 1 for every glass;
(5) to drench after having inserted water 1 time, make substrate water content in nutrition cup reach about 80%, and cover with bamboo cane shed transparent plastic film and carry out moisturizing;
(6) shade with sunshade net scaffolding, avoid direct sunlight, the light transmittance of canopy is 30%;
(7) opened film spraying fungicide every 7 days and prevent mould from growing after inserting, cover film again after spray;
(8) when more than 90% rooting of cuttings and taking out sprout blade aging after throw off the plastic film of covering, manage nursery stock afterwards until go out garden and transplant.
2. one side pin cuttage and seedling culture method according to claim 1, is characterized in that the bactericide of described step (2) and (7) is the 0.5% carbendazim aqueous solution.
3. one side pin cuttage and seedling culture method according to claim 1, is characterized in that the immersion treatment time of described step (2) is 20 minutes, has soaked rear water purification and rinsed.
4. one side pin cuttage and seedling culture method according to claim 1, is characterized in that the plant hormone of described step (3) is the hormotone aqueous solution that indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) are mixedly configured into by a certain percentage.
5. one side pin cuttage and seedling culture method according to claim 4, it is characterized in that the compound method of the described hormotone aqueous solution first uses appropriate 95% alcohol indole-3-butyric acid (IBA) and methyl α-naphthyl acetate (NAA) to be dissolved respectively, adding distil water is configured to the hormone solution of 10g/L respectively again, measure two kinds of hormone solution by volume respectively to mix, finally add the distilled water mixing constant volume of 22/3 times.
6. one side pin cuttage and seedling culture method according to claim 1, is characterized in that the immersion treatment method of described step (3) is that long for cutting lower end 1/3 part is soaked 1 hour.
7. one side pin cuttage and seedling culture method according to claim 1, is characterized in that the seedbed of described step (4) is the smooth furrow face of making wide 1.2m, high 10cm with thick river sand.
8. one side pin cuttage and seedling culture method according to claim 1, is characterized in that the matrix of the nutrition cup of described step (4) is mixed with 2 ~ 3 parts of peats composed of rotten mosses, 2 parts of red heart soil and 1 ~ 2 part of coconut palm chaff that becomes thoroughly decomposed to form.
CN201510067365.0A 2015-02-10 2015-02-10 Cutting seedling method for zanthoxylum dissitum Expired - Fee Related CN104604512B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106718544A (en) * 2016-12-05 2017-05-31 广西壮族自治区桂林茶叶科学研究所 A kind of tea cutting urges the method for culturing seedlings of root
CN107409925A (en) * 2017-09-06 2017-12-01 广西壮族自治区林业科学研究院 A kind of method of the asexual fast breeding of Moringa
CN108966877A (en) * 2018-07-10 2018-12-11 思南县华丰果蔬专业合作社 A kind of method for culturing seedlings of cherry tree

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101099433A (en) * 2007-08-06 2008-01-09 柳州两面针股份有限公司 Reproduction method of plant Zanthoxylum Nitidum
CN102293116A (en) * 2011-07-12 2011-12-28 广西壮族自治区林业科学研究院 Fast culture method for camellia oleifera light substrate container cuttage seedlings
CN102986437A (en) * 2012-12-14 2013-03-27 茂名市天一农业科技发展有限公司 Method for cultivating radix zanthoxyli artificially

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101099433A (en) * 2007-08-06 2008-01-09 柳州两面针股份有限公司 Reproduction method of plant Zanthoxylum Nitidum
CN102293116A (en) * 2011-07-12 2011-12-28 广西壮族自治区林业科学研究院 Fast culture method for camellia oleifera light substrate container cuttage seedlings
CN102986437A (en) * 2012-12-14 2013-03-27 茂名市天一农业科技发展有限公司 Method for cultivating radix zanthoxyli artificially

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
梁淑娈等: "花椒育苗技术", 《农村科技开发》 *
牛丙午: "花椒绿枝扦插育苗", 《河北林业科技》 *
王丽敏: "小花花椒生态学特性及繁殖技术研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *
赵建民等: "花椒种子与扦插育苗技术", 《中国林副特产》 *
韦树德: "花椒扦插育苗技术", 《北方园艺》 *
黄开顺等: "蚬壳花椒种质资源与栽培研究进展", 《广西林业科学》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106718544A (en) * 2016-12-05 2017-05-31 广西壮族自治区桂林茶叶科学研究所 A kind of tea cutting urges the method for culturing seedlings of root
CN107409925A (en) * 2017-09-06 2017-12-01 广西壮族自治区林业科学研究院 A kind of method of the asexual fast breeding of Moringa
CN108966877A (en) * 2018-07-10 2018-12-11 思南县华丰果蔬专业合作社 A kind of method for culturing seedlings of cherry tree

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