CN104764640A - Chloral hydrate permeabilization flaking method in microscopic identification of TCM - Google Patents

Chloral hydrate permeabilization flaking method in microscopic identification of TCM Download PDF

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CN104764640A
CN104764640A CN201510123975.8A CN201510123975A CN104764640A CN 104764640 A CN104764640 A CN 104764640A CN 201510123975 A CN201510123975 A CN 201510123975A CN 104764640 A CN104764640 A CN 104764640A
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chloral hydrate
potpourri
saturatingization
mass concentration
microslide
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刘孟奇
扶垭东
高鹏
王小巧
陈随清
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Henan University of Traditional Chinese Medicine HUTCM
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Henan University of Traditional Chinese Medicine HUTCM
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Abstract

The invention relates to a chloral hydrate permeabilization flaking method in microscopic identification of TC, which can effectively solve the problem of easy control, high efficiency and safety of chloral hydrate permeabilization. The method comprises the following steps: adding water in chloral hydrate for dissolving to obtain a chloral hydrate solution with mass concentration of 30-50%; adding 0.1-0.5g of traditional Chinese medicinal material powder into 2ml of chloral hydrate solution with mass concentration of 30-50% to prepare a mixture; filling the mixture into a container, preheating to 100-110 DEG C, then heating to 125-135 DEG C for 4-8 minutes, realizing permeabilization of the mixture; dropping the 1-2 drops of permeabilized mixture on a slide glass, adding 1-2 drops of dilute glycerin, uniformly mixing the traditional Chinese medicinal material powder on the slide glass, covering a coverslip, using an absorbent paper to remove overflowed liquid, and keeping the clean coverslip and slide glass. The flaking method can be effectively used for chloral hydrate permeabilization, and has the advantages of energy saving, safety, high efficiency, controllable performance, wide application and environmental protection, is especially suitable for teaching and scientific research, has strong utility value, and is great innovation of chloral hydrate permeabilization flaking method.

Description

In a kind of TCD MICROSCOPIC IDENTIFICATION, chloral hydrate changes the method for film-making thoroughly
Technical field
The present invention relates to traditional Chinese medicine quality to detect, particularly in a kind of TCD MICROSCOPIC IDENTIFICATION, chloral hydrate changes the method for film-making thoroughly.
Background technology
It is normal now technical operation in Chinese traditional medicine identification method that hydration atmosphere aldehyde changes film-making thoroughly, and the quality of film-making directly has influence on qualification.But along with the development need of teaching and scientific research, this traditional saturatingization mode deficiency displays gradually, except using except spirit lamp occurs that naked light is dangerous in test, in the process making load, also easily there is the situations such as heating is uneven, produce bubble, powder is scorched, have impact on accuracy and the conventional efficient of experiment to a great extent.
It is a kind of material processing method the most often used in TCD MICROSCOPIC IDENTIFICATION that chloral hydrate changes method of tableting thoroughly.Principle: chloral hydrate is good clarifier, can infiltrate rapidly powder organization's cell, strengthens penetrability, is convenient to observe.But in long-term practice, this traditional saturatingization mode deficiency displays gradually.Use spirit lamp to there is naked light danger in experiment, in film-making, difficult control of temperature easily occurs that heating is uneven, and the situations such as powder is scorched, and easily produces bubble, the easy sooting microslide of spirit lamp flame, have impact on the clear observation of microscopic morphology to a great extent.Chloral hydrate heat releases poisonous gas, is poisonous substances, and irritant.Especially in the experimental teaching of traditional Chinese medicine class universities and colleges, many people are long-term in the classroom of an improper ventilation often uses, and can work the mischief to Faculty and Students, and not easily manipulate, efficiency is low, poor stability.Therefore, how improvement and bring new ideas chloral hydrate changes method of tableting is thoroughly the technical matters that must solve.
Summary of the invention
For above-mentioned situation, for overcoming the defect that prior art exists, the object of the present invention is just to provide chloral hydrate in new a kind of TCD MICROSCOPIC IDENTIFICATION and thoroughly changes the method for film-making, effectively can solve chloral hydrate and thoroughly change easy control, efficient, safe problem.
The technical scheme that the present invention solves first installs firing equipment, then thoroughly changes film-making according to the following steps:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 30-50%;
(2) potpourri of Chinese drugs powder and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 30-50% that 0.1-0.5g Chinese drugs powder is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 100-110 DEG C, then be warming up to 125-135 DEG C, heating 4-8min, realizes saturatingization to potpourri;
(4) film-making after saturatingization: the potpourri 1-2 of step (3) saturatingization is dripped and (often drips 0.05ml, be designated as 0.05ml/ to drip) drop in (the wide 25.4mm of microslide, long 76.2mm on microslide, thick 1mm-1.2mm), drip 1-2 again to drip and (often drip 0.05ml, be designated as 0.05ml/ to drip) rare glycerine of mass concentration 15-25%, by the Chinese drugs powder mixing on microslide, covered, the liquid of spilling is sucked with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making;
Described firing equipment comprises casing, casing top is provided with airtight heating zone 4 and open heating zone 5 side by side, airtight heating zone 4 is provided with the heating tank 4a placing heating container, casing front portion is disposed with display 1, pilot lamp 2, power switch 3, attaching plug 10 is equipped with through wire in the rear portion of casing, the casing left and right sides is respectively equipped with vent window 11, box house is equipped with well heater (JRQ) A, relay 6, temperature controller 9, temperature sensor 8 and isolating switch (QF) B form heating arrangement, well heater is placed in the casing below airtight heating zone 4 and open heating zone 5, temperature controller 9 is synthermal sensor 8 respectively, display 1, relay 6 is connected, and be connected with isolating switch B through wire, well heater one end is connected with relay, the other end is connected with isolating switch B through joint 7, isolating switch is connected with attaching plug 10 through power switch 3, power switch 3 is connected with the pilot lamp 2 before casing,
Described Chinese drugs powder is the root of herbaceous peony (root), adhesive rehmannia leaf, Chinese yam (root-like stock), the red sage root (root), Chinese drugs powder that a kind of pulverizing of honeysuckle (flower) etc. is made (are only provide several Chinese medicine embodiment here, Chinese drugs powder can also be the powder of other different Chinese crude drugs prepared as required, does not enumerate).
Method of tableting of the present invention is effective to chloral hydrate and thoroughly changes method, energy-conservation, safe, efficient, controlled, wide application, environmental protection, is particularly suitable for teaching and scientific research, has very strong practical value, be that chloral hydrate is thoroughly changed one on method of tableting and innovated greatly, economic and social benefit is huge.
Accompanying drawing explanation
Fig. 1 is the three-dimensional structure diagram of firing equipment of the present invention.
Fig. 2 is the circuit connection structure figure of firing equipment of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the invention is elaborated.
The present invention, in concrete enforcement, can be provided by following examples.
Embodiment 1
The present invention, in concrete enforcement, first installs firing equipment, then completes chloral hydrate in TCD MICROSCOPIC IDENTIFICATION according to the following steps and thoroughly change film-making:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 30%;
(2) potpourri of White Peony Root and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 30% that 0.1g White Peony Root is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 100 DEG C, then be warming up to 125 DEG C, heating 6min, realizes saturatingization to potpourri;
(4) film-making after saturatingization: the potpourri 0.05ml of step (3) saturatingization is dropped in (25.4 × 76.2mm on microslide, thickness 1mm-1.2mm), drip rare glycerine of the mass concentration 15% of 0.05ml again, by the White Peony Root mixing on microslide, covered, suck the liquid of spilling with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
Embodiment 2
The present invention, in concrete enforcement, first installs firing equipment, then thoroughly changes film-making according to the following steps:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 35%;
(2) potpourri of adhesive rehmannia leaf powder and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 35% that 0.2g adhesive rehmannia leaf powder is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 105 DEG C, then be warming up to 130 DEG C, heating 7min, realizes saturatingization to potpourri;
(4) film-making after saturatingization: the potpourri 0.07ml of step (3) saturatingization is dropped in (25.4 × 76.2mm on microslide, thickness 1mm-1.2mm), drip rare glycerine of the mass concentration 20% of 0.07ml again, by the glutinous rehmannia powder mixing on microslide, covered, suck the liquid of spilling with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
Embodiment 3
The present invention in force, first installs firing equipment, then thoroughly changes film-making according to the following steps:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 40%;
(2) potpourri of Chinese yam powder and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 40% that 0.3g Chinese yam powder is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 110 DEG C, then be warming up to 135 DEG C, heating 8min, realizes saturatingization to potpourri;
(4) film-making after saturatingization: the potpourri 0.1ml of step (3) saturatingization is dropped in (25.4 × 76.2mm on microslide, thickness 1mm-1.2mm), drip rare glycerine of the mass concentration 25% of 0.1ml again, by the Chinese yam powder mixing on microslide, covered, suck the liquid of spilling with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
Embodiment 4
The present invention in force, first installs firing equipment, then thoroughly changes film-making according to the following steps:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 45%;
(2) potpourri of Danshen Root and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 45% that 0.4g Danshen Root is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 100 DEG C, then be warming up to 125 DEG C, heating 5min, realizes saturatingization to potpourri;
(4) film-making after saturatingization: the potpourri 0.06ml of step (3) saturatingization is dropped in (25.4 × 76.2mm on microslide, thickness 1mm-1.2mm), drip rare glycerine of the mass concentration 17% of 0.06ml again, by the Chinese drugs powder mixing on microslide, covered, suck the liquid of spilling with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
Embodiment 5
First firing equipment is installed, then thoroughly changes film-making according to the following steps:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 50%;
(2) potpourri of Honeysuckle Flower and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 50% that 0.5g Chinese drugs powder is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 105 DEG C, then be warming up to 125 DEG C, heating 4min, realizes saturatingization to potpourri;
(4) film-making after saturatingization: the potpourri 0.1ml of step (3) saturatingization is dropped in (25.4 × 76.2mm on microslide, thickness 1mm-1.2mm), drip rare glycerine of the mass concentration 23% of 0.1ml again, by the Honeysuckle Flower mixing on microslide, covered, suck the liquid of spilling with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
The above-mentioned embodiment provided is only for illustration of the specific embodiment of the present invention; not limit the scope of the invention, therefore, everyly to change in technical scheme; but its essence is identical with technical scheme of the present invention, all belongs to the scope of request of the present invention protection.
The inventive method is simple, easy to operate, fundamentally changes the heating of existing employing spirit lamp and carries out chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change the method for film-making, be widely used in teaching and scientific research, energy-conservation, efficient, environmental protection, compared with prior art, has following outstanding Advantageous Effects:
safety: effectively avoid spirit lamp to heat and occur the potential safety hazard that naked light exists.
efficient: to avoid coking, heating is uneven and produce bubble phenomenon, greatly reduces raw-material waste, saves material more than 40%, and be easy to operation, can produce abundant sample at short notice, efficiency improves more than 4 times.
controlled: owing to have employed special firing equipment, utilize equipment to carry out digital display and temperature control, conveniently adjusted temperature, ensure that saturatingization is abundant, thoroughly change film-making to realize high-quality chloral hydrate, improve the quality of teaching and scientific research.
be suitable for wide: owing to have employed special firing equipment, be provided with the alternative heating zone of closed and two, open heating zone in firing equipment, therefore, be very beneficial for utilizing different vessels thoroughly to change process to different Chinese drugs powder, applicability is wide, and efficiency improves 2-3 doubly.
environmental protection: add heat penetration process in the bottle of sealing, significantly reduce toxic gas chloral hydrate and evaporate in air, reduce harmful gas to the pollution of environment and the infringement to person health, safety, economic and social benefit is huge.

Claims (6)

1. in TCD MICROSCOPIC IDENTIFICATION, chloral hydrate changes a method for film-making thoroughly, it is characterized in that, first installs firing equipment, then carries out chloral hydrate according to the following steps and thoroughly changes film-making:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 30-50%;
(2) potpourri of Chinese drugs powder and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 30-50% that 0.1-0.5g Chinese drugs powder is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 100-110 DEG C, then 125-135 DEG C is warming up to, heating 4-8min, realizes saturatingization to Chinese drugs powder;
(4) mounting after saturatingization: the potpourri 1-2 after step (3) saturatingization is dropped on microslide, drip rare glycerine of the mass concentration 15-25% that 1-2 drips again, by the herb powder mixing on microslide, covered, the liquid of spilling is sucked with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making;
Described firing equipment comprises casing, casing top is provided with airtight heating zone (4) and open heating zone (5) side by side, airtight heating zone (4) is provided with the heating tank (4a) placing heating container, casing front portion is disposed with display (1), pilot lamp (2), power switch (3), attaching plug (10) is equipped with through wire in the rear portion of casing, the casing left and right sides is respectively equipped with vent window (11), box house is equipped with well heater (A), relay (6), temperature controller (9), temperature sensor (8) and isolating switch (B) form heating arrangement, well heater is placed in the casing of airtight heating zone (4) and open heating zone (5) below, temperature controller (9) is synthermal sensor (8) respectively, display (1), relay (6) is connected, and be connected with isolating switch (B) through wire, well heater one end is connected with relay, the other end is connected with isolating switch (B) through joint (7), isolating switch is connected with attaching plug (10) through power switch (3), power switch (3) is connected with the pilot lamp (2) before casing,
Described Chinese drugs powder is a kind of Chinese drugs powder made of the root of herbaceous peony, adhesive rehmannia leaf, Chinese yam, the red sage root, honeysuckle.
2. in TCD MICROSCOPIC IDENTIFICATION according to claim 1, chloral hydrate changes the method for film-making thoroughly, it is characterized in that, first installs firing equipment, then completes chloral hydrate in TCD MICROSCOPIC IDENTIFICATION according to the following steps and thoroughly changes film-making:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 30%;
(2) potpourri of White Peony Root and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 30% that 0.1g White Peony Root is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 100 DEG C, then be warming up to 125 DEG C, heating 6min, realizes saturatingization to Chinese drugs powder;
(4) mounting after saturatingization: the potpourri 0.05ml of step (3) saturatingization is dropped on microslide, drip rare glycerine of the mass concentration 15% of 0.05ml again, by the White Peony Root mixing on microslide, covered, the liquid of spilling is sucked with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
3. in TCD MICROSCOPIC IDENTIFICATION according to claim 1, chloral hydrate changes the method for film-making thoroughly, it is characterized in that, first installs firing equipment, then carries out chloral hydrate according to the following steps and thoroughly changes film-making:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 35%;
(2) potpourri of glutinous rehmannia powder and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 35% that 0.2g glutinous rehmannia powder is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 105 DEG C, then be warming up to 130 DEG C, heating 7min, realizes saturatingization to Chinese drugs powder;
(4) mounting after saturatingization: the potpourri 0.07ml of step (3) saturatingization is dropped on microslide, drip rare glycerine of the mass concentration 20% of 0.07ml again, by the glutinous rehmannia powder mixing on microslide, covered, the liquid of spilling is sucked with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
4. in TCD MICROSCOPIC IDENTIFICATION according to claim 1, chloral hydrate changes the method for film-making thoroughly, it is characterized in that, first installs firing equipment, then carries out chloral hydrate according to the following steps and thoroughly changes film-making:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 40%;
(2) potpourri of Chinese yam powder and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 40% that 0.3g Chinese yam powder is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 110 DEG C, then be warming up to 135 DEG C, heating 8min, realizes saturatingization to Chinese drugs powder;
(4) mounting after saturatingization: the potpourri 0.1ml of step (3) saturatingization is dropped on microslide, drip rare glycerine of the mass concentration 25% of 0.1ml again, by the Chinese yam powder mixing on microslide, covered, the liquid of spilling is sucked with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
5. in TCD MICROSCOPIC IDENTIFICATION according to claim 1, chloral hydrate changes the method for film-making thoroughly, it is characterized in that, first installs firing equipment, then carries out chloral hydrate according to the following steps and thoroughly changes film-making:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 45%;
(2) potpourri of Danshen Root and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 45% that 0.4g Danshen Root is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 100 DEG C, then be warming up to 125 DEG C, heating 5min, realizes saturatingization to Chinese drugs powder;
(4) mounting after saturatingization: the potpourri 0.06ml of step (3) saturatingization is dropped on microslide, drip rare glycerine of the mass concentration 17% of 0.06ml again, by the Danshen Root mixing on microslide, covered, the liquid of spilling is sucked with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
6. in TCD MICROSCOPIC IDENTIFICATION according to claim 1, chloral hydrate changes the method for film-making thoroughly, it is characterized in that, first installs firing equipment, then carries out chloral hydrate according to the following steps and thoroughly changes film-making:
(1) chloral hydrate solution is prepared: chloral hydrate is dissolved in water into the chloral hydrate solution that mass concentration is 50%;
(2) potpourri of Honeysuckle Flower and chloral hydrate solution is prepared: it is in the chloral hydrate solution 2ml of 50% that 0.5g honeysuckle material powder is joined mass concentration, makes potpourri;
(3) heat penetration is added to potpourri: loaded by potpourri in container, put into the heating tank of the airtight heating zone of firing equipment or open heating zone, start firing equipment, first be preheated to 105 DEG C, then be warming up to 125 DEG C, heating 4min, realizes saturatingization to Chinese drugs powder;
(4) mounting after saturatingization: the potpourri 0.1ml of step (3) saturatingization is dropped on microslide, drip rare glycerine of the mass concentration 23% of 0.1ml again, by the Honeysuckle Flower mixing on microslide, covered, the liquid of spilling is sucked with thieving paper, keep cover glass and microslide cleaning, namely complete chloral hydrate in TCD MICROSCOPIC IDENTIFICATION and thoroughly change film-making.
CN201510123975.8A 2015-03-21 2015-03-21 Chloral hydrate permeabilization flaking method in microscopic identification of TCM Pending CN104764640A (en)

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CN105241740A (en) * 2015-11-20 2016-01-13 河南中医学院 Closed heating device used for permeabilizing and dyeing in traditional Chinese medicine microscopic flaking
CN105241740B (en) * 2015-11-20 2017-11-03 河南中医学院 It is a kind of to be used for the airtight heating device of permeabilization and dyeing in Chinese medicine microsection
CN106769306A (en) * 2016-12-19 2017-05-31 山东天博食品配料有限公司 A kind of improved spice microscopic features observational technique
CN106769306B (en) * 2016-12-19 2019-08-02 山东中质华检测试检验有限公司 A kind of improved spice microscopic features observation method
CN110161156A (en) * 2018-02-13 2019-08-23 特丰制药有限公司 The method of efficient liquid phase measurement chloraldurate content
CN110161156B (en) * 2018-02-13 2022-12-09 特丰制药有限公司 Method for determining content of chloral hydrate by high performance liquid phase
CN108426882A (en) * 2018-02-22 2018-08-21 广西壮族自治区柳州食品药品检验所 A kind of DANQI PIAN supplement detection method

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