CN106769306A - A kind of improved spice microscopic features observational technique - Google Patents
A kind of improved spice microscopic features observational technique Download PDFInfo
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- CN106769306A CN106769306A CN201611176640.3A CN201611176640A CN106769306A CN 106769306 A CN106769306 A CN 106769306A CN 201611176640 A CN201611176640 A CN 201611176640A CN 106769306 A CN106769306 A CN 106769306A
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- spice
- microscopic features
- observation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
Abstract
The present invention relates to a kind of improved spice microscopic features observational technique, step is as follows：Spice substance is crushed using pulverizer, and crosses 80 mesh sieves, obtain condiment powder；Take a little condiment powder to be placed on slide, chloraldurate solution 1~2 is added dropwise and drips, stand；The unnatural flowing of liquid on slide is slowly heated to using alcolhol burner, then it is 1 that volume ratio is added dropwise:1 glycerite 1 drips, volume ratio is 1:1 dilute iodine solution 1 is dripped, and after being mixed using sharp instruments, adds a cover slide；Observed with low power objective, found image, selected the image of primary part observation, observed with high power objective, you can observation is obtained the microscopic features of spice.This method is contaminated with iodine using low concentration chloraldurate saturatingization and is combined, can not only quickly, comprehensively, clear, accurate observation spice microscopic features, the especially spice higher to content of starch, and avoiding excessively and easily separates out the problem of crystallization, significantly reduce amount of reagent, low cost, safety.
Description
Technical field
The invention belongs to food technology field, especially a kind of improved spice microscopic features observational technique.
Background technology
Biological each tissue morphology is respectively provided with relatively stable microscopic features, even pulverized, its tissue, cell,
Inclusion etc. is still visible, and it is to differentiate the basis of spice to understand and grasp these essential characteristics.
In document at present on the observation of spice microscopic features, the method for microscopic features observation is first with high concentration
Chloraldurate solution processes spice sample, and run-of-the-mill concentration is more than 15%, then carries out micro- sem observation, but high concentration
Chloraldurate solution easily cause cell permeabilization excessively, or even chloraldurate separate out crystallization, have a strong impact on observing effect, increase
Experimental implementation difficulty.
Chloraldurate is a kind of chemical reagent, with pungent acrid odors, the solution of high concentration can make body produce compared with
The sense of discomfort of weight, harm is produced to health, and amount of reagent is big, also increases experimental cost.In some literature methods
Also require to be dried with alcolhol burner during observation, this operation is more easy to make chloraldurate separate out crystallization, and is also easy to produce bubble, no
Beneficial to microexamination.Existing observational technique when content of starch spice higher is observed, such as rhizoma zingiberis, the root of Dahurain angelica, kaempferia galamga, no
Energy is clear, accurately observe starch granule structure, with certain limitation, comprehensively and accurately understand such perfume (or spice) so as to have impact on
The microscopic features of pungent material.
By retrieval, the patent publication us related to present patent application are not yet found.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art part, there is provided a kind of improved spice microscopic features are seen
Method is examined, the method is contaminated with iodine using low concentration chloraldurate saturatingization and is combined, can not only quickly, comprehensively, clearly, accurately
Observation spice microscopic features, the especially spice higher to content of starch, and avoiding excessively and easily separates out knot
Brilliant problem, significantly reduces amount of reagent, low cost, safety.
The present invention solves its technical problem and takes following technical scheme to realize：
A kind of improved spice microscopic features observational technique, step is as follows：
(1) spice sample treatment：Spice substance is crushed using pulverizer, and crosses 80 mesh sieves, obtain condiment powder；
(2) prepared by observation sample：Take a little condiment powder to be placed on slide, the hydration that mass concentration is 1~5% is added dropwise
Chloral solution 1~2 drips, and stands 1~3min；The unnatural flowing of liquid on slide is slowly heated to using alcolhol burner, but should not be steamed
It is dry, then it is 1 that volume ratio is added dropwise:1 glycerite 1 drips, volume ratio is 1:1 dilute iodine solution 1 is dripped, after being mixed using sharp instruments,
Add a cover slide；
(3) micro- sem observation：First observed with low power objective, found image, selected the image of primary part observation, used instead height
Times object lens observation, you can observation obtains the microscopic features of spice.
And, (2) middle sharp instruments are toothpick to the step.
And, the solvent of the step (2) middle chloraldurate solution is distilled water, and the solvent of glycerite is distilled water, dilute
The solvent of iodine solution is distilled water.
And, (3) middle high power objective is 100 times of object lens to the step, is oil mirror, it is necessary to cedar oil is added dropwise.
Advantages and positive effects of the present invention are：
The inventive method improves existing spice microscopic features observational technique, uses low concentration chloraldurate saturatingization and iodine
Dye is combined, can not only quickly, comprehensively, clear, accurate observation spice microscopic features, it is especially higher to content of starch
Spice, can become apparent from, accurately observe starch granule structure, and avoiding excessively and easily separates out asking for crystallization
Topic, significantly reduces amount of reagent, low cost, safety.
Specific embodiment
With reference to embodiment, the present invention is further described；Following embodiments are illustrative, be not it is limited,
Protection scope of the present invention can not be limited with following embodiments.
Raw material used in the present invention, unless otherwise specified, is conventional commercially available prod；Used in the present invention
Method, unless otherwise specified, is the conventional method of this area.
Embodiment 1
A kind of anistree microscopic features observational technique of improved spice, step is as follows：
(1) anistree sample treatment：Anistree substance is crushed using pulverizer, and crosses 80 mesh sieves, obtain star aniseed powder.
(2) prepared by observation sample：Take a little star aniseed powder to be placed on slide, the chloraldurate that mass concentration is 1% is added dropwise
Solution 2 drips, and stands 2min, the unnatural flowing of liquid (should not be evaporated) on slide is slowly heated to using alcolhol burner, then body is added dropwise
Product is than being 1:1 glycerite 1 drips, volume ratio is 1:1 dilute iodine solution 1 is dripped, and after being mixed using sharp instruments such as toothpicks, adds a cover glass
Piece.
(3) micro- sem observation：First observed with low power objective, found image, selected the image of primary part observation, used instead
100 times of object lens observations (100 times of object lens are oil mirror, it is necessary to cedar oil is added dropwise), you can observation obtains the anistree micro- spy of spice
Levy.
Can clearly, be quickly observed anise microscopic features：
Containing a large amount of light yellow circular oil droplets, endocarp palisade cells cylindricality long, wall is slightly thick, pit aperture crosswise or herringbone
Shape, some cell membranes are thickened into V-shaped.The prothenchyma (of wood)s such as a large amount of lithocytes, lithocyte is faint yellow, yellowish-brown, and it is many that class is seen on surface
Angular, wall is extremely thick, and wavy bending, cell is branched, includes brownish black thing.A small amount of conduit, based on screw thread, there is a small amount of starch
Grain, single amylum body is rounded or Long Circle, and some have herringbone omphalion in circular amylum body, without laminated striation.Some amylum bodies
Aggregation is agglomerating, with less guard cell, stomata.
Embodiment 2：
A kind of microscopic features observational technique of improved spice rhizoma zingiberis, step is as follows：
(1) rhizoma zingiberis sample treatment：Rhizoma zingiberis substance is crushed using pulverizer, and crosses 80 mesh sieves, obtain ginger powder.
(2) prepared by observation sample：Take a little ginger powder to be placed on slide, the chloraldurate that mass concentration is 5% is added dropwise
Solution 1 drips, and stands 3min, the unnatural flowing of liquid (should not be evaporated) on slide is slowly heated to using alcolhol burner, then body is added dropwise
Product is than being 1:1 glycerite 1 drips, volume ratio is 1:1 dilute iodine solution 1 is dripped, and after being mixed using sharp instruments such as toothpicks, adds a cover glass
Piece.
(3) micro- sem observation：First observed with low power objective, found image, selected the image of primary part observation, used instead
100 times of object lens observations (100 times of object lens are oil mirror, it is necessary to cedar oil is added dropwise), you can obtain the microscopic features of spice rhizoma zingiberis.
Can clearly, be quickly observed the microscopic features of rhizoma zingiberis：
Amylum body is numerous, and circular or side protrudes beak shape, point-like or slit-like omphalion, slightly laminated striation.Omphalion point-like,
Positioned at small end, also have in slit-like person, it is obvious that laminated striation has.Eleocyte and resin cell are dissipated in parenchymal tissue, are included light
Yellow oil droplet or reddish dark brown material.Fiber bunchy is dissipated from, tip blunt point, and a small number of bifurcateds, some one side is in wavy or sawtooth
Shape, wall is slightly thick, non-woodization, has oblique microgroove hole, Chang Kejian poor tabula.Scalariform duct, spiral duct and reticulate vessel are common,
Minority is annular duct.A large amount of calcium oxalate crystals, based on prismatic crystal, there is larger prismatic crystal, also there is tiny prismatic crystal
Embodiment 3
A kind of improved spice microscopic features observational technique, step is as follows：
(1) spice sample treatment：Spice substance is crushed using pulverizer, and crosses 80 mesh sieves, obtain condiment powder；
(2) prepared by observation sample：Take a little condiment powder to be placed on slide, the hydration that mass concentration is 1~5% is added dropwise
Chloral solution 1~2 drips, and stands 1~3min；The unnatural flowing of liquid on slide is slowly heated to using alcolhol burner, but should not be steamed
It is dry, then it is 1 that volume ratio is added dropwise:1 glycerite 1 drips, volume ratio is 1:1 dilute iodine solution 1 is dripped, after being mixed using sharp instruments,
Add a cover slide；
(3) micro- sem observation：First observed with low power objective, found image, selected the image of primary part observation, used instead height
The observation of times object lens, you can clearly, be quickly observed the microscopic features of spice；
(2) middle sharp instruments are toothpick to the step；
The solvent of the step (2) middle chloraldurate solution is distilled water, and the solvent of glycerite is distilled water, dilute iodine solution
Solvent be distilled water.
The above, is only presently preferred embodiments of the present invention, and any form is not made to technical scheme
On limitation.Every any simple modification made to above example according to technical spirit of the invention, equivalent variations and repair
Decorations, still fall within the range of technical scheme.
Claims (4)
1. a kind of improved spice microscopic features observational technique, it is characterised in that：Step is as follows：
(1) spice sample treatment：Spice substance is crushed using pulverizer, and crosses 80 mesh sieves, obtain condiment powder；
(2) prepared by observation sample：Take a little condiment powder to be placed on slide, the chloraldurate that mass concentration is 1~5% is added dropwise
Solution 1~2 drips, and stands 1~3min；The unnatural flowing of liquid on slide is slowly heated to using alcolhol burner, but should not be evaporated, then
It is 1 that volume ratio is added dropwise:1 glycerite 1 drips, volume ratio is 1:1 dilute iodine solution 1 is dripped, and after being mixed using sharp instruments, adds a cover glass
Piece；
(3) micro- sem observation：First observed with low power objective, found image, selected the image of primary part observation, used instead high power thing
Sem observation, you can observation obtains the microscopic features of spice.
2. improved spice microscopic features observational technique according to claim 1, it is characterised in that：The step (2) in
Sharp instruments are toothpick.
3. improved spice microscopic features observational technique according to claim 1, it is characterised in that：The step (2) in
The solvent of chloraldurate solution is distilled water, and the solvent of glycerite is distilled water, and the solvent of dilute iodine solution is distilled water.
4. the improved spice microscopic features observational technique according to any one of claims 1 to 3, it is characterised in that：Institute
(3) middle high power objective is 100 times of object lens to state step, is oil mirror, it is necessary to cedar oil is added dropwise.
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| CN201611176640.3A CN106769306B (en) | 2016-12-19 | 2016-12-19 | A kind of improved spice microscopic features observation method |
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| CN201611176640.3A CN106769306B (en) | 2016-12-19 | 2016-12-19 | A kind of improved spice microscopic features observation method |
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| CN106769306A true CN106769306A (en) | 2017-05-31 |
| CN106769306B CN106769306B (en) | 2019-08-02 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021102949A1 (en) * | 2019-11-29 | 2021-06-03 | 深圳迈瑞生物医疗电子股份有限公司 | Cell morphology analysis device, cell recognition method, and storage medium |
| CN115372088A (en) * | 2022-07-04 | 2022-11-22 | 河北省药品医疗器械检验研究院(河北省化妆品检验研究中心) | Method for manufacturing medicinal material transverse slice capable of clearly observing cell wall structure |
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| CN101706404A (en) * | 2008-11-03 | 2010-05-12 | 河南大学 | Method for identifying crude drug powder |
| CN101943665A (en) * | 2009-07-10 | 2011-01-12 | 华北制药集团制剂有限公司 | Method for detecting quality of wild jujube barks |
| CN102768209A (en) * | 2012-08-06 | 2012-11-07 | 河南中医学院 | Method for observing microscopic structures inside plant roots |
| CN104764640A (en) * | 2015-03-21 | 2015-07-08 | 河南中医学院 | Chloral hydrate permeabilization flaking method in microscopic identification of TCM |
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2016
- 2016-12-19 CN CN201611176640.3A patent/CN106769306B/en active Active
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| CN101706404A (en) * | 2008-11-03 | 2010-05-12 | 河南大学 | Method for identifying crude drug powder |
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| CN102768209A (en) * | 2012-08-06 | 2012-11-07 | 河南中医学院 | Method for observing microscopic structures inside plant roots |
| CN104764640A (en) * | 2015-03-21 | 2015-07-08 | 河南中医学院 | Chloral hydrate permeabilization flaking method in microscopic identification of TCM |
| CN105004726A (en) * | 2015-08-04 | 2015-10-28 | 江苏科技大学 | Phellinus identification method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021102949A1 (en) * | 2019-11-29 | 2021-06-03 | 深圳迈瑞生物医疗电子股份有限公司 | Cell morphology analysis device, cell recognition method, and storage medium |
| CN115372088A (en) * | 2022-07-04 | 2022-11-22 | 河北省药品医疗器械检验研究院(河北省化妆品检验研究中心) | Method for manufacturing medicinal material transverse slice capable of clearly observing cell wall structure |
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| CN106769306B (en) | 2019-08-02 |
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Effective date of registration: 20190618 Address after: 272073 Lunan Quality Inspection Center, No. 16 Huanhe Road, Jining High-tech Zone, Shandong Province Applicant after: Shandong China Quality Inspection & Testing Co., Ltd. Address before: 272100 6 Hai Chuan Road, Jie Zhuang Street, Jining hi tech Zone, Shandong Applicant before: Shandong Tianbo Food Ingredients Co., Ltd. |
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