CN108426882A - A kind of DANQI PIAN supplement detection method - Google Patents
A kind of DANQI PIAN supplement detection method Download PDFInfo
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- CN108426882A CN108426882A CN201810153974.1A CN201810153974A CN108426882A CN 108426882 A CN108426882 A CN 108426882A CN 201810153974 A CN201810153974 A CN 201810153974A CN 108426882 A CN108426882 A CN 108426882A
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- China
- Prior art keywords
- danqi
- danqi pian
- pian
- plant tissues
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
Abstract
The present invention relates to a kind of DANQI PIANs to supplement detection method, includes the following steps：DANQI PIAN 2 is taken, coating is removed, it is finely ground, fine powder 0.1g is weighed, water 10ml is added to make dissolving, is centrifuged, discard supernatant liquid, precipitation plus water 2ml make dissolving, shake up, and draw the drop of suspension 1 on glass slide, chloraldurate test solution 1 is added to drip, permeabilization is heated, is set under 100 times of microscope, 9 checkpoints is chosen on the cover slip and inspects；When in addition to Radix Notoginseng crude drug microscopic features, other plant tissues are not detected in the visual field, then assert that the production technology of DANQI PIAN meets the requirements；When only there are one other plant tissues are detected in the visual field, then taking DANQI PIAN to carry out retrial 1 time again again, the checkpoint of preliminary examination and retrial detection containing other plant tissues is more than 1, then assert that the production technology of DANQI PIAN does not meet the requirements.Compared with the prior art, during the present invention can screen production DANQI PIAN, whether red rooted salvia is in strict accordance with requiring to be used as medicine with decocting liquid, if other pollutants brought into.
Description
Technical field
The present invention relates to detection technique field, more particularly to a kind of DANQI PIAN supplements detection method.
Background technology
DANQI PIAN is made of Radix Salviae Miltiorrhizae and two taste medicine of Radix Notoginseng, is《Chinese Pharmacopoeia》Record kind.
【Preparation method】Radix Notoginseng is ground into fine powder；Radix Salviae Miltiorrhizae adds water to cook three times, 1 hour every time, and decocting liquid filtration, filtrate merges, dense
Be reduced to appropriate, Radix Notoginseng powder and starch, appropriate dextrin be added, mixing is dry, compacting, sugar coating or film-coating to get.
The two taste medicinal material containing Radix Notoginseng and Radix Salviae Miltiorrhizae in we, wherein Radix Salviae Miltiorrhizae are added water to cook, are used as medicine with concentrate, and Radix Notoginseng is ground into carefully
Powder is directly used as medicine with powder, therefore can observe the microscopic features of Radix Notoginseng crude drug under the microscope, and cannot be detected in addition to Radix Notoginseng
The plant tissue feature of (including Radix Salviae Miltiorrhizae).《Chinese Pharmacopoeia》In one DANQI PIAN of version in 2015【Differentiate】(1) requirement under item：It takes
This product sets microscopically observation：Resin canal fragment secretion containing yellow (Radix Notoginseng).The discriminating item only requires the resin for checking Radix Notoginseng
Whether road fragment can detect, illegal production in order to prevent without regulation Radix Salviae Miltiorrhizae crude drug and other plant tissue features
Producer's disregard of law regulation simplifies legal production technology, Radix Salviae Miltiorrhizae is directly added in powder form, what this standard was established【It is anisotropic
Organic matter】Whether inspection method, the production technology that can screen DANQI PIAN meet legal requirements.
Invention content
The object of the present invention is to provide a kind of DANQI PIANs to supplement detection method, the technical problem to be solved is that：In order to anti-
Only illegal manufacturer's disregard of law regulation simplifies legal production technology, Radix Salviae Miltiorrhizae is directly added in powder form.
The technical solution that the present invention solves above-mentioned technical problem is as follows：A kind of DANQI PIAN supplement detection method, including it is following
Step：
DANQI PIAN 2 is taken, coating is removed, it is finely ground, fine powder 0.1g is weighed, water 10ml is added to make dissolving, centrifuges, discards supernatant
Liquid, precipitation plus water 2ml make dissolving, shake up, and draw the drop of suspension 1 on glass slide, adding chloraldurate test solution 1 to drip, heat permeabilization,
It sets under 100 times of microscope, chooses 9 checkpoints on the cover slip and inspect；When in the visual field in addition to Radix Notoginseng crude drug microscopic features, not
Other plant tissues are detected, then assert that the production technology of DANQI PIAN meets the requirements；When only there are one detect other plants in the visual field
Tissue then takes DANQI PIAN to carry out retrial 1 time again again, and the checkpoint of preliminary examination and retrial detection containing other plant tissues is more than 1,
Then assert that the production technology of DANQI PIAN does not meet the requirements.
The beneficial effects of the invention are as follows：Pharmaceutical factory can be screened during producing the kind, whether red rooted salvia is stringent
According to legal manufacturing technique requirent with decocting liquid be used as medicine and production link in dirt visual under other microscopes that may bring into
Object is contaminated, quality standard and specification pharmaceutical technology to improve the medicine provide detection foundation.
Description of the drawings
Fig. 1 is checkpoint schematic diagram on coverslip of the present invention.
Specific implementation mode
The principle and features of the present invention will be described below with reference to the accompanying drawings, and the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
A kind of DANQI PIAN supplement detection method, includes the following steps：
1, instrument：DM500 microscopic imaging apparatus, Leca microscopic system Co., Ltd
Electronic analytical balance Merttler AE200, plum Teller Instrument Ltd. of Switzerland
2, reagent：Chloraldurate, Sinopharm Chemical Reagent Co., Ltd.
Three-level detection water, Chongqing mole Molelement companies
3, sample DANQI PIAN sample table
4, control medicinal material：
1. Radix Salviae Miltiorrhizae, lot number 120923-200610, source：Chinese pharmaceutical biological product examines and determine research institute
2. Radix Notoginseng, lot number 120941-201409, source：Chinese pharmaceutical biological product examines and determine research institute
5, detection method：DANQI PIAN 2 is taken, coating is removed, it is finely ground, fine powder 0.1g is weighed, water 10ml is added to make dissolving, from
The heart, discards supernatant liquid, and precipitation plus water 2ml make dissolving, shake up, and draws the drop of suspension 1 on glass slide, adding chloraldurate test solution 1
Drop heats permeabilization, sets under 100 times of microscope, is inspected as shown in Figure 1, choosing 9 checkpoints on the cover slip；When being removed in the visual field
Outside Radix Notoginseng crude drug microscopic features, other plant tissues are not detected, then assert that the production technology of DANQI PIAN meets the requirements；When only
Other plant tissues are detected in one visual field, then DANQI PIAN is taken to carry out retrial 1 time again again, preliminary examination and retrial detection contain other plants
The checkpoint of object tissue is more than 1, then assert that the production technology of DANQI PIAN does not meet the requirements.
6, testing result takes 16 batches of DANQI PIAN samples and Radix Salviae Miltiorrhizae control medicinal material and Radix Notoginseng control medicinal material, according to above-mentioned detection side
Method is detected, and is set and is observed under 100 times of microscope, and is imaged by DM500 microscopic imaging apparatus, and micro-image is obtained；
According to《Chinese Pharmacopoeia》Version in 2015 one and Pharmacopoeia of People's Republic of China herb powder microscopical characters cromogram
Collect [4], the microscopic features of Notoginseng Root have：Resin canal secretion containing yellow；It can be seen that scalariform duct, reticulate vessel and screw thread are led
Pipe；Amylum body has simple grain, composite grain, a lot of；Calcium oxalate cluster crystal is rare.The microscopic features of Danshen Root have：Lithocyte similar round, class
Triangle, rectangle like or irregular shape, some extensions are in threadiness, and edge out-of-flatness, hole ditch is apparent, and some cells include Huang
Brown object；Wood-fibred spindle shape, end beveled tip or blunt circle, pit oblique segmentation gap-like or cross；It can be seen that reticulate vessel and tool edge line
Hole conduit.Through testing, in the sample of 16 batches of different lot numbers of 6 production units, the DANQI PIAN of 5 producers such as A, C, D, E and F is examined
Go out the powder characteristics of medicinal material Radix Notoginseng --- resin canal, scalariform duct, reticulate vessel, spiral duct and accidental calcium oxalate cluster crystal (because
Sample powder water dissolution, and chloraldurate reagent is added to heat permeabilization, therefore amylum body has become paste), in addition, others are not detected
Histocyte feature.The lot number of B producers production is 151101,150419,160109,160501,161201 and 170302 etc. 6 batches
In secondary DANQI PIAN, other than detecting Radix Notoginseng crude drug tissue, it was found that wood-fibred, fibre bundle and lithocyte etc. are other to plant
Object tissue signature, wherein the wood-fibred tool spindle shape detected in the batches such as 150419,160501,170302, pit oblique segmentation gap-like
And the features such as lithocyte similar round in 160109, the microscopic features with red rooted salvia are very much like.We contain Radix Notoginseng and Radix Salviae Miltiorrhizae
Two taste medicinal materials, wherein Radix Salviae Miltiorrhizae are added water to cook, are used as medicine with concentrate, and Radix Notoginseng is ground into fine powder, is directly used as medicine with powder, therefore micro-
Under the microscope, other than the microscopic features of Notoginseng Root, Radix Salviae Miltiorrhizae and other plant tissue features cannot be detected.Collect the 6 of B producers
It criticizes in sample, it is found that 5 batches have plant tissue of the detection in addition to Radix Notoginseng, from 2015 to 2017 in year continuous 3 years samples
There is detection, however not excluded that the factory simplifies legal production technology, and red rooted salvia is directly added in powder form；Or the factory is producing
During the kind, there are the cross contamination of raw material, the pharmacy health of the cleaning of instrument and equipment and other production links is not
Legal requirements etc. can be reached.
The DANQI PIAN that this standard is established supplements detection method, can screen pharmaceutical factory during producing the kind, Radix Salviae Miltiorrhizae
Medicinal material whether in strict accordance with legal manufacturing technique requirent with decocting liquid be used as medicine and production link in may bring into other are micro-
Visual pollutant under mirror, quality standard and specification pharmaceutical technology to improve the medicine provide detection foundation.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (1)
1. a kind of DANQI PIAN supplements detection method, which is characterized in that include the following steps：
DANQI PIAN 2 is taken, coating is removed, it is finely ground, fine powder 0.1g is weighed, water 10m l is added to make dissolving, centrifuges, discards supernatant liquid, is sunk
It forms sediment plus water 2ml makes dissolving, shake up, draw the drop of suspension 1 on glass slide, adding chloraldurate test solution 1 to drip, heat permeabilization, set aobvious
Under 100 times of micro mirror, 9 checkpoints are chosen on the cover slip and are inspected；When in addition to Radix Notoginseng crude drug microscopic features, being not detected in the visual field
Other plant tissues then assert that the production technology of DANQI PIAN meets the requirements；When only there are one detecting other plant tissues in the visual field,
DANQI PIAN is then taken to carry out retrial 1 time again again, the checkpoint of preliminary examination and retrial detection containing other plant tissues is more than 1, then recognizes
The production technology for determining DANQI PIAN does not meet the requirements.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810153974.1A CN108426882A (en) | 2018-02-22 | 2018-02-22 | A kind of DANQI PIAN supplement detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810153974.1A CN108426882A (en) | 2018-02-22 | 2018-02-22 | A kind of DANQI PIAN supplement detection method |
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| CN108426882A true CN108426882A (en) | 2018-08-21 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762422A (en) * | 2005-10-20 | 2006-04-26 | 北京汉典中西药研究开发中心 | Process for preparing soft capsule of red sage root and notoginseng |
| CN102353735A (en) * | 2011-07-13 | 2012-02-15 | 贵阳中医学院第二附属医院 | Quality detection method for Tongmai Tangyanming capsule |
| CN104764640A (en) * | 2015-03-21 | 2015-07-08 | 河南中医学院 | Chloral hydrate permeabilization flaking method in microscopic identification of TCM |
-
2018
- 2018-02-22 CN CN201810153974.1A patent/CN108426882A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762422A (en) * | 2005-10-20 | 2006-04-26 | 北京汉典中西药研究开发中心 | Process for preparing soft capsule of red sage root and notoginseng |
| CN102353735A (en) * | 2011-07-13 | 2012-02-15 | 贵阳中医学院第二附属医院 | Quality detection method for Tongmai Tangyanming capsule |
| CN104764640A (en) * | 2015-03-21 | 2015-07-08 | 河南中医学院 | Chloral hydrate permeabilization flaking method in microscopic identification of TCM |
Non-Patent Citations (3)
| Title |
|---|
| WANGYUAN: "丹七片", 《医学百科》 * |
| 王文洁等: "《药物检测技术》", 31 January 2017 * |
| 肖冰梅等: "复方丹参胶囊的显微及理化鉴别质量研究", 《中华中医药学会第九届中药鉴定学术会议论文集》 * |
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Application publication date: 20180821 |
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