CN110771601B - Fresh plant specimen and preparation method thereof - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/06—Pectin; Derivatives thereof
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/02—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
- C08L2205/025—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group containing two or more polymers of the same hierarchy C08L, and differing only in parameters such as density, comonomer content, molecular weight, structure
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/03—Polymer mixtures characterised by other features containing three or more polymers in a blend
- C08L2205/035—Polymer mixtures characterised by other features containing three or more polymers in a blend containing four or more polymers in a blend
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Abstract
The invention relates to the technical field of plant specimen processing, in particular to a fresh plant specimen and a preparation method thereof, wherein a solid specimen mainly adopts a colloidal substance to replace resin which is not environment-friendly and light-resistant in the prior art as an embedding agent, and the embedding agent is preferably selected, and specifically comprises the following components: carrageenan, pectin, aloe vera gel and jelly powder; simultaneously adding antibacterial agent aloe extract and potassium sorbate into the colloid; the specimen plant is embedded by the embedding medium and then sealed and subjected to ultraviolet sterilization, so that the sterilization effect can be effectively realized, a vacuum sterile state is formed inside the specimen, the specimen plant can be effectively protected, and the anti-photoaging effect can also be realized.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of plant specimen processing, in particular to a fresh plant specimen and a preparation method thereof.
[ background of the invention ]
The original color undisturbed specimen of the plant plays an important role in the plant research of botany, also has important value in aspects such as specimen display, exhibition and artistic appreciation, etc., at present, the solid embedding of the fresh plant mainly adopts resin to embed, for example, Chinese patent CN 109085028A "manufacturing method of citrus leaf disease solidified specimen" the method adopts epoxy resin and curing agent to embed; the resin has strong curing capability and low cost, and becomes the mainstream of embedding the plant specimen, but the resin is easy to change color and become brittle under the irradiation of sunlight, most of the plant specimens embedded by the resin at present are required to be not placed outdoors for display, or cannot be irradiated by the sunlight, and are required to be dried and stored in dark, which brings great inconvenience to the display of the plant specimen, and the resin material is not environment-friendly.
[ summary of the invention ]
In view of the above, it is necessary to develop a solid specimen to produce an embedding material capable of adapting to normal temperature, normal light and normal humidity environment, so as to embed fresh plant specimen without causing color loss of plants; the plant specimen has good anti-photoaging effect.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a fresh plant specimen is a solid specimen and is prepared by processing fresh plants through a color retention solution and then embedding the fresh plants in a solid embedding medium.
Further, the solid color-retaining liquid is prepared by mixing a solid-liquid ratio of 1: 20, preparing the copper sulfate solid and acetic acid into a stock solution, and diluting by 4 times.
Further, the solid embedding medium is prepared from the following components: the aloe gel consists of carrageenan, pectin, aloe gel, aloe polysaccharide, jelly powder, aloe extract and potassium sorbate according to the mass ratio of 2:2-5:2-5:1:3-5:1: 1.
The present invention also provides a method of preparing a fresh plant specimen, the method comprising the steps of:
(1) selecting fresh plants without diseases and insect pests, cleaning and removing soil;
(2) weighing copper sulfate solid and glacial acetic acid according to the solid-liquid ratio; mixing to obtain stock solution; when in use, adding 4 times of water to dilute the plant into color preservation solution, putting the fresh plant in the step (1) into the color preservation solution, heating the plant to 70-80 ℃, stopping heating when the plant is observed to change from green to yellow and then to green, taking out the fresh plant, putting the fresh plant into clear water to wash the plant clean, and preserving the plant in preservation solution;
(3) weighing the embedding agent components according to the mass ratio, and then mixing the embedding agent components according to the solid-liquid ratio of 1:3, measuring an aqueous solution, adding carrageenan, pectin, aloe gel, aloe polysaccharide and jelly powder into water, fully stirring, heating to boil, continuing to heat for 10min, then cooling to 70-75 ℃, continuing to heat at constant temperature until the solution becomes viscous, and then adding an aloe extract and potassium sorbate, and fully and uniformly stirring to obtain an embedding agent solution;
(4) taking the plant in the preservation solution in the step (2) out of the preservation solution, and draining;
(5) pouring the embedding agent solution obtained in the step (3) into a specimen container, pouring 1/3-1/2 with the volume being the specimen container, fully spreading the drained plants in the embedding agent, pouring the embedding agent solution until the whole container is full, sealing the specimen container, and irradiating the whole container under an ultraviolet lamp until the temperature is cooled to room temperature;
(6) after cooling to room temperature, filling glue in the seams of the specimen container, and then putting the specimen container into a refrigerating chamber of a refrigerator for refrigerating for 24-26 h; and taking out the specimen container, and filling glue water at the joint of the specimen container to obtain the fresh plant specimen.
Further, the preservation solution in the step (2) is composed of lemon juice, barbaloin, honey and water according to the mass ratio of 1-3:2-4:1: 100.
Further, the wavelength of the ultraviolet lamp in the step (5) is 100nm-300 nm.
The fresh plant preserved in the application is radix ophiopogonis.
The invention has the following beneficial effects:
1. the application's solid specimen mainly adopts colloidal substance to replace not environmental protection among the prior art, not resin of light resistance as the embedding agent, and the preferred food level of embedding agent glues specifically to be: carrageenan, pectin, aloe vera gel and jelly powder; in our study of the preparation of specimens using a colloid-substituted resin, it was found that: the colloid is soft, although the colloid is not easy to be aged by light, the adopted colloid is mostly food-grade colloid which contains a plurality of nutrients available for microorganisms, if the mixture ratio is not good, the phenomena of deterioration and wilting of the plant are influenced, the appearance of the plant is influenced, and the primary color and the state of the plant cannot be well protected, so that the inventor also adds the aloe extract and the potassium sorbate with antibacterial effect; in order to improve the absorption wave band and diffuse reflection effect of the embedding agent on illumination, the inventor also adds aloe polysaccharide for proportioning to prepare the embedding agent component of the application; at present, the inventor cannot well explain why the original color and state of the plant can be kept by adopting the above-mentioned ratio, and the inventor believes that different colloids and substances have different light absorption wave bands and different diffuse reflection effects, and the change of the absorbed wavelength after the colloids and the substances are combined together finally causes different embedded objects to be affected differently by the light conditions so as to affect the appearance of the plant specimen differently, but the inventor can be sure that the addition of the aloe extract and the potassium sorbate with the sterilization effect in the colloid substances can improve the antibacterial property of the specimen and reduce the harm of microorganisms to the plant specimen; the inventor carries out aging observation on a plant specimen exposed under a UVB lamp condition, and finds that the ultraviolet lamp cannot play a sterilization role with the increase of time, so that mildew spots are generated on the surface of the specimen with incomplete mixture ratio, and therefore, the applicant adds the ultraviolet lamp as an embedding agent and can effectively play an antibacterial effect; protecting the specimen plants and maintaining the original color and state of the specimen plants; certainly, the inventor still needs to seal when preparing the sample, carries out ultraviolet sterilization after sealing, more effectively plays the bactericidal effect, makes the inside vacuum sterile state that forms of sample, can effectively protect the sample plant.
2. This application adopts two segmentations to seal when sealing, scribbles glue earlier, scribbles glue again after cold-stored, utilizes the difference in temperature to make glue paste more firmly, can be fine play sealed effect.
[ description of the drawings ]
FIG. 1 is a specimen diagram of an embodiment of the invention;
FIG. 2 is a diagram of a specimen prepared by a conventional preparation method.
[ detailed description ] embodiments
All of the features disclosed in this specification, or all of the steps in any method or process so disclosed, may be combined in any combination, except combinations of features and/or steps that are mutually exclusive.
Any feature disclosed in this specification (including any accompanying claims, abstract) is merely an example of a generic series of equivalent or similar features, unless explicitly described as such.
Example 1:
the fresh plant selected in the embodiment is radix ophiopogonis; the preparation method comprises the following steps:
(1) selecting fresh radix Ophiopogonis with roots and without plant diseases and insect pests, pulling up, cleaning and removing soil;
(2) weighing 5g of copper sulfate solid, weighing 100mL of glacial acetic acid, and uniformly mixing to prepare a stock solution; when in use, adding 4 times of water to dilute into color retention solution, placing the radix ophiopogonis in the step (1) into the color retention solution, heating to 70 ℃, stopping heating when the leaves of the plant are observed to change from green to yellow and then to green, taking out the fresh plant, putting the fresh plant into clear water to wash the fresh plant, and storing the fresh plant in storage solution (wherein the preparation method of the storage solution is that the storage solution consists of lemon juice, barbaloin, honey and water according to the mass ratio of 1:2:1: 100);
(3) weighing embedding agent components (200 g in total) according to the mass ratio of carrageenan, pectin, aloe gel, aloe polysaccharide, jelly powder, aloe extract and potassium sorbate of 2:2:2:1:3:1:1, and then mixing the embedding agent components according to the solid-liquid ratio of 1:3, measuring an aqueous solution (namely 600mL of the aqueous solution), adding carrageenan, pectin, aloe gel, aloe polysaccharide and jelly powder into water, fully stirring, heating to boil, continuing to heat for 10min, cooling to 70 ℃, continuing to heat at constant temperature until the solution becomes viscous, then adding an aloe extract and potassium sorbate, and fully and uniformly stirring to obtain an embedding agent solution;
(4) taking out the radix ophiopogonis in the preserving fluid in the step (2), and draining;
(5) pouring the embedding agent solution obtained in the step (3) into a specimen container, pouring 1/3 with the volume being the specimen container, fully spreading the drained radix ophiopogonis in the embedding agent, pouring the embedding agent solution until the whole container is full, sealing the specimen container, and irradiating the whole container under an ultraviolet lamp with the wavelength of 300nm until the container is cooled to room temperature;
(6) after cooling to room temperature, filling glue in the seams of the specimen container, and then putting the specimen container into a refrigerating chamber of a refrigerator for refrigerating for 24-26 h; and taking out the specimen container, and filling glue water at the joint of the specimen container to obtain the fresh plant specimen.
Example 2:
the fresh plant selected in the embodiment is radix ophiopogonis; the preparation method comprises the following steps:
(1) selecting fresh radix Ophiopogonis with roots and without plant diseases and insect pests, pulling up, cleaning and removing soil;
(2) weighing 5g of copper sulfate solid, weighing 100mL of glacial acetic acid, and uniformly mixing to prepare a stock solution; when in use, adding 4 times of water to dilute into color retention solution, placing the radix ophiopogonis in the step (1) into the color retention solution, heating to 80 ℃, stopping heating when the leaves of the plant are observed to change from green to yellow and then to green, taking out the fresh plant, putting the fresh plant into clear water to wash the fresh plant, and storing the fresh plant in the preservation solution (wherein, the preservation solution is prepared by the steps that the preservation solution consists of lemon juice, barbaloin, honey and water according to the mass ratio of 3:4:1: 100);
(3) weighing embedding agent components (200 g in total) according to the mass ratio of carrageenan, pectin, aloe gel, aloe polysaccharide, jelly powder, aloe extract and potassium sorbate of 2:5:5:1:5:1:1, and then mixing the embedding agent components according to the solid-liquid ratio of 1:3, measuring an aqueous solution (namely 600mL of the aqueous solution), adding carrageenan, pectin, aloe gel, aloe polysaccharide and jelly powder into water, fully stirring, heating to boil, continuing to heat for 10min, cooling to 75 ℃, continuing to heat at constant temperature until the solution becomes viscous, then adding an aloe extract and potassium sorbate, and fully and uniformly stirring to obtain an embedding agent solution;
(4) taking out the radix ophiopogonis in the preserving fluid in the step (2), and draining;
(5) pouring the embedding agent solution obtained in the step (3) into a specimen container, pouring 1/2 with the volume being the specimen container, fully spreading the drained radix ophiopogonis in the embedding agent, pouring the embedding agent solution until the whole container is full, sealing the specimen container, and placing the whole container under an ultraviolet lamp with the wavelength of 100nm for irradiation until the container is cooled to room temperature;
(6) after cooling to room temperature, filling glue in the seams of the specimen container, and then putting the specimen container into a refrigerating chamber of a refrigerator for refrigerating for 24-26 h; and taking out the specimen container, and filling glue water at the joint of the specimen container to obtain the fresh plant specimen.
Example 3:
the fresh plant selected in the embodiment is radix ophiopogonis; the preparation method comprises the following steps:
(1) selecting fresh radix Ophiopogonis with roots and without plant diseases and insect pests, pulling up, cleaning and removing soil;
(2) weighing 5g of copper sulfate solid, weighing 100mL of glacial acetic acid, and uniformly mixing to prepare a stock solution; when in use, adding 4 times of water to dilute into color preservation solution, putting the radix ophiopogonis in the step (1) into the color preservation solution, heating to 75 ℃, stopping heating when the leaves of the plant are observed to change from green to yellow and then to green, taking out the fresh plant, putting the fresh plant into clear water to wash the fresh plant, and preserving the fresh plant in the preservation solution (wherein, the preservation solution is prepared by the steps that the preservation solution consists of lemon juice, barbaloin, honey and water according to the mass ratio of 2:3:1: 100);
(3) weighing embedding agent components (200 g in total) according to the mass ratio of carrageenan, pectin, aloe gel, aloe polysaccharide, jelly powder, aloe extract and potassium sorbate of 2:4:4:1:4:1:1, and then mixing the embedding agent components according to the solid-liquid ratio of 1:3, measuring an aqueous solution (namely 600mL of the aqueous solution), adding carrageenan, pectin, aloe gel, aloe polysaccharide and jelly powder into water, fully stirring, heating to boil, continuing to heat for 10min, cooling to 72 ℃, continuing to heat at constant temperature until the solution becomes viscous, then adding an aloe extract and potassium sorbate, and fully and uniformly stirring to obtain an embedding agent solution;
(4) taking out the radix ophiopogonis in the preserving fluid in the step (2), and draining;
(5) pouring the embedding agent solution obtained in the step (3) into a specimen container, pouring 1/3 with the volume being the specimen container, fully spreading the drained radix ophiopogonis in the embedding agent, pouring the embedding agent solution until the whole container is full, sealing the specimen container, and placing the whole container under an ultraviolet lamp with the wavelength of 200nm for irradiation until the container is cooled to room temperature;
(6) after cooling to room temperature, filling glue in the seams of the specimen container, and then putting the specimen container into a refrigerating chamber of a refrigerator for refrigerating for 24-26 h; and taking out the specimen container, and filling glue water at the joint of the specimen container to obtain the fresh plant specimen.
The jelly powders of examples 1-3 were all purchased from the market under the brand name: the collected tea aroma specification is 1000 g/bag.
The extraction method of aloe vera extract of examples 1-3 was: grinding aloe, mixing with 75% ethanol according to a solid-liquid mass ratio of 1:2, leaching for 48h, and filtering; then mixing the filter residue with 75% ethanol in a solid-to-liquid ratio of 1:1, and leaching for 24 h; mixing the filtrates, concentrating by rotary evaporation, and removing ethanol to obtain Aloe extract.
Photo-aging performance study:
experimental groups: preparing a plant specimen by adopting the method of example 1, but not sealing the cover, namely filling the container with the embedding medium, then not sealing the container, opening the cover, directly placing the container into an aging box for light irradiation after the embedding medium is condensed;
control group 1: the control group 1 was identical to the experimental group except that the embedding agent was selected: weighing embedding medium components (total 200g) according to the mass ratio of the aloe gel, the aloe polysaccharide, the jelly powder, the aloe extract and the potassium sorbate being 2:1:3:1: 1;
control group 2: the control group 2 was performed in the same manner as the experimental group, except that the embedding agent was selected: weighing embedding medium components (total 200g) according to the mass ratio of carrageenan, pectin, aloe gel, aloe polysaccharide, jelly powder and aloe extract being 2:2:2:1:3: 1;
control group 3: the control group 3 was identical to the experimental group except that the embedding agent was selected: weighing embedding medium components (total 200g) according to the mass ratio of the carrageenan, the pectin, the aloe gel, the aloe polysaccharide and the jelly powder of 2:2:2:1: 3;
control group 4: the control group 4 was performed in the same manner as the experimental group except that the embedding agent was selected: weighing embedding medium components (total 200g) according to the mass ratio of carrageenan, pectin, aloe gel, jelly powder, aloe extract and potassium sorbate being 2:2:2:1:1: 1;
control group 5: the control group 5 was identical to the experimental group except that the embedding agent was selected: the control group 3 was identical to the experimental group except that the embedding agent was selected: epoxy resin (total 200 g).
And (3) CK group: fresh plants are treated by the preservation solution and then directly placed in an aging box for illumination.
The lighting condition of the aging box is set as follows: the UVB lamp continuously irradiates at 400nm without interruption;
observing the shapes and color senses of the embedding agent surface and the specimen plants after 2h, 24h, 48h, 120h and 240h of irradiation, wherein the specific conditions are shown in a table 1:
TABLE 1
As can be seen from the above table, the specimens of the experimental group have no obvious changes in form and color sensation during the experimental period; the appearance of the specimens of the control groups 1-3 shows mildew spots in 240 hours, and the plants are affected to show the phenomena of blackening and wilting; the shape of the plant is changed under the influence of illumination without obvious change; the control group 5 not only has the change of the plant appearance, but also has the aging of the appearance of the specimen, and has the phenomena of yellowing and white powder, which are probably caused by the aging of resin materials and the breakage of molecular bonds due to illumination; CK group does not have specimen material parcel, and the deformation is rotten very fast.
As shown in FIG. 1, which is a diagram of a fresh radix Ophiopogonis specimen prepared by the preparation method of example 1, the specimen has clear outline and good shape retention.
FIG. 2 is a specimen soaked by the conventional preservative (formaldehyde), and the specimen is unclear in outline, serious in decoloration and incapable of well maintaining the appearance of the specimen.
In conclusion, the embedding agent combination has strong light aging resistance, can effectively protect the specimen from being influenced by illumination conditions, adopts purely natural food-grade materials, and is an environment-friendly, safe, efficient and light-resistant specimen embedding agent and a specimen preparation method.
The above examples are merely illustrative of several embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (2)
1. A method of preparing a fresh plant specimen, the method comprising the steps of:
(1) selecting fresh plants without diseases and insect pests, cleaning and removing soil;
(2) according to the solid-liquid ratio of 1: 20, uniformly mixing copper sulfate solid and acetic acid to prepare stock solution; when in use, adding 4 times of water to dilute the plant into color preservation solution, putting the fresh plant in the step (1) into the color preservation solution, heating the plant to 70-80 ℃, stopping heating when the plant is observed to change from green to yellow and then to green, taking out the fresh plant, putting the fresh plant into clear water to wash the plant clean, and preserving the plant in preservation solution;
(3) weighing the embedding agent components according to the mass ratio of carrageenan, pectin, aloe gel, aloe polysaccharide, jelly powder, aloe extract and potassium sorbate of 2:2-5:2-5:1:3-5:1:1, and then mixing the components according to the solid-liquid ratio of 1:3, measuring an aqueous solution, adding carrageenan, pectin, aloe gel, aloe polysaccharide and jelly powder into water, fully stirring, heating to boil, continuing to heat for 10min, then cooling to 70-75 ℃, continuing to heat at constant temperature until the solution becomes viscous, and then adding an aloe extract and potassium sorbate, and fully and uniformly stirring to obtain an embedding agent solution;
(4) taking the plant in the preservation solution in the step (2) out of the preservation solution, and draining;
(5) pouring the embedding agent solution obtained in the step (3) into a specimen container, pouring 1/3-1/2 with the volume being the specimen container, fully spreading the drained plants in the embedding agent, pouring the embedding agent solution until the whole container is full, sealing the specimen container, and irradiating the whole container under an ultraviolet lamp until the temperature is cooled to room temperature;
(6) after cooling to room temperature, filling glue in the seams of the specimen container, and then putting the specimen container into a refrigerating chamber of a refrigerator for refrigerating for 24-26 h; taking out and then filling glue water at the joint of the specimen container to obtain a fresh plant specimen;
the preserving fluid in the step (2) is composed of lemon juice, barbaloin, honey and water according to the mass ratio of 1-3:2-4:1: 100;
the plant is radix Ophiopogonis.
2. The method of claim 1, wherein the ultraviolet lamp of step (5) has a wavelength of 100nm to 300 nm.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0822403A1 (en) * | 1996-08-02 | 1998-02-04 | Milestone S.r.l. | Process for processing organic specimens |
| CN102187857A (en) * | 2011-04-02 | 2011-09-21 | 仲恺农业工程学院 | Method for protecting color and keeping shape of China loropetal leaves |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
| CN103583509A (en) * | 2013-11-27 | 2014-02-19 | 青岛海洋科技馆 | Making method of gel specimen |
| WO2016084094A1 (en) * | 2014-11-30 | 2016-06-02 | Yissum Research Develoment Company Of The Hebrew University Of Jerusalem Ltd. | Edible morpholine-free coating formulations |
| CN108552164A (en) * | 2018-05-25 | 2018-09-21 | 宁德师范学院 | A kind of semisolid store method of plant specimen |
| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
-
2019
- 2019-11-21 CN CN201911147118.6A patent/CN110771601B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0822403A1 (en) * | 1996-08-02 | 1998-02-04 | Milestone S.r.l. | Process for processing organic specimens |
| CN102187857A (en) * | 2011-04-02 | 2011-09-21 | 仲恺农业工程学院 | Method for protecting color and keeping shape of China loropetal leaves |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
| CN103583509A (en) * | 2013-11-27 | 2014-02-19 | 青岛海洋科技馆 | Making method of gel specimen |
| WO2016084094A1 (en) * | 2014-11-30 | 2016-06-02 | Yissum Research Develoment Company Of The Hebrew University Of Jerusalem Ltd. | Edible morpholine-free coating formulations |
| CN108552164A (en) * | 2018-05-25 | 2018-09-21 | 宁德师范学院 | A kind of semisolid store method of plant specimen |
| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
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