CN111183976A - Preparation method for preparing wax leaf specimen by using refrigeration pressurization method - Google Patents
Preparation method for preparing wax leaf specimen by using refrigeration pressurization method Download PDFInfo
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- CN111183976A CN111183976A CN202010206742.5A CN202010206742A CN111183976A CN 111183976 A CN111183976 A CN 111183976A CN 202010206742 A CN202010206742 A CN 202010206742A CN 111183976 A CN111183976 A CN 111183976A
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- specimen
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/38—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for botany
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a preparation method for manufacturing a wax leaf specimen by using a refrigeration pressurization method, which is characterized in that the process for manufacturing the wax leaf specimen by using a traditional natural drying method is improved and innovated, the fresh plant is picked and pressed into the specimen, and the whole process is kept at 1-6 ℃ for refrigeration; the traditional specimen folder with the equal scale enlargement is 40cm in length, 35cm in width, 4cm in width and 2.5cm in thickness to increase the pressure resistance; the thickness of the finished specimen holder can be reduced by 3 times by using a screw with the thickness of 0.3cm and the diameter of 1 cm. Compared with the traditional natural drying pressing method, the method of the invention is simpler, lower in cost, easy to learn and labor-saving, the shape of the obtained specimen is consistent with that of the specimen during collection, and the color is almost unchanged.
Description
Technical Field
The invention belongs to the field of specimen preparation, and particularly relates to a preparation method for preparing a wax leaf specimen by using a refrigeration pressurization method.
Background
The wax leaf specimen is a necessary teaching material in the process of plant teaching of Chinese medicines, is a transmitter of important information in scientific research, and is a path for inheriting, innovating, displaying and propagating Chinese traditional medicine culture. A specimen with good quality can vividly explain the important characteristics of the specimen without being limited by regions and time, how to keep the original appearance of plants and what method can prolong the retention time of the natural color of a wax leaf specimen, and is the subject of intense research of many Chinese medical personnel.
The traditional natural drying method for preparing the pressed specimen is simple, low in cost and good in preparation effect. But the manufacturing process needs to change the absorbent paper and arrange the specimen for many times, and the binding of the specimen holder is very labor-consuming; the traditional specimen clamp and the binding tool only play a role in fixing the specimen, the purpose of completely drying the specimen cannot be achieved, and the obtained finished product is withered and yellow in color and even has the phenomena of rotting, worm eating and the like.
Technical scheme
The invention aims to provide a preparation method for preparing a wax leaf specimen by using a refrigeration pressurization method, and the invention is an innovative specimen preparation method which is simpler, lower in price, easy to learn and labor-saving than the traditional pressing method; meanwhile, the yield of the specimen preparation and the quality of the specimen can be improved.
The technical scheme of the invention is as follows:
the method is improved and innovated on the basis of the traditional specimen pressing process by a natural drying method, and discloses a preparation method for manufacturing a wax leaf specimen by using a refrigeration pressurization method, which comprises the following steps:
firstly, storing the fresh plants collected in the field in a heat preservation box at the temperature of 1-6 ℃.
Secondly, the collected plant specimens are arranged on a specimen holder after being fresh, the middle of the plant specimens is separated by absorbent paper, and corrugated paper is used between the plant specimens and the specimen holder.
Thirdly, the collected specimen is compressed in a specimen holder; the scaling-up traditional specimen holder has the dimensions of 40cm in length, 35cm in width and 4cm in width, 2.5cm in thickness, so that the compression resistance of the specimen holder is improved, and the collected specimen is compressed in the specimen holder as much as possible. The binding belt is changed into a triangular iron: the thickness of the finished specimen holder can be reduced by 3 times by using screws with the thickness of 0.3cm of triangular iron and the diameter of 1cm of the screws to apply pressure to the specimen holder. And (4) storing the pressed specimen in a refrigerator at 1-6 ℃.
Fourthly, storing the pressed specimen in a refrigerator at the temperature of 1-6 ℃.
And fifthly, changing the dried absorbent paper the next day, and rearranging the specimen. And (4) pressing the specimen holder by using a screw and a triangular iron again, and storing in a refrigerator at the temperature of 1-6 ℃ until the specimen is completely dried.
The arrangement in the second step comprises the following steps: pruning dead leaves on fresh plants, standing the roots in water for a moment, and washing back and forth to remove mud; cutting the tuber or root into transverse section and longitudinal section. Cutting the tuber or root into pieces with proper thickness, keeping the surplus part or all of the pieces, and pressing together.
In the third step, the finished thick and thin ends of the plants are placed on the absorbent paper at the bottoms of the specimen holders and the corrugated paper pad in a mutually staggered manner, branches and leaves are spread out, the front and back sides of the leaves are placed, overlong herbaceous or liana plants are bent in the shapes of 'N', 'V' and 'w', 2 or 3 pieces of dry absorbent paper are placed among the plant specimens, 5-10 pieces of absorbent paper and corrugated paper are placed on the top, the other specimen holder is placed on the specimen holder, and the specimen holder is screwed to the top by triangular iron and screws.
Compared with the prior art, the invention has the technical advantages that:
firstly, the size of the traditional specimen holder is enlarged in equal proportion, the intact shape of the fresh plant is kept, and the secondary cutting is carried out after the drying, so that more ideal size and shape are obtained.
Two, use angle iron and screw fixation specimen holder, can reduce 3 times with the specimen holder thickness of putting in order, make the specimen laminate completely in the paper that absorbs water, reduce the inside air of specimen holder, the plant tissue damage that makes through the extrusion lets moisture wherein be absorbed the paper and dry rapidly.
Thirdly, a new strategy of pressing the specimen by a refrigeration pressurization method is provided for the first time, and the defects that the color of the specimen is accelerated by adopting heat treatment drying and the color of the specimen becomes unreal by a chemical reagent method in the traditional method are improved. Based on the principle that the medicinal materials are stored in a refrigerator for refrigeration and can reduce moisture, the plant tissues can be damaged by matching with pressurization, so that the moisture of the specimen is quickly sucked dry, and the mildew and the germination are avoided.
And fourthly, the number of times of paper replacement by the traditional pressing method is about 8, and only one time of paper replacement is needed by using a refrigeration pressurization method, so that the labor intensity is reduced.
And fifthly, the color of the finished product of the specimen is almost close to that of the fresh plant after the process is improved.
Detailed Description
The present invention will be further illustrated below with reference to specific embodiments, which are to be understood as merely illustrative and not limitative of the scope of the present invention.
Example 1
The working principle of the invention is that the plant to be pressed is stored at low temperature (1-6 ℃) in the whole process from collection to specimen preparation; the pressure of the specimen holder is increased by improving the specimen holder and the binding tool, so that the water is quickly absorbed by the absorbent paper after the plant tissue is damaged.
Discloses a preparation method for making a wax leaf specimen by using a refrigeration pressurization method, which comprises the following steps:
firstly, collecting rhizoma corydalis, radix pseudostellariae and rhizoma arisaematis (including root, stem, leaf, flower, fruit or seed), numbering and tagging, and filling field records in detail. The materials are simply cleaned and then placed in a heat preservation box at the temperature of 1-6 ℃ to keep the natural state of the materials as much as possible.
Secondly, pruning dead leaves on fresh plants, standing the roots of the medicinal materials in water for a moment, and washing back and forth to remove mud; cutting the tuber or root into transverse section and longitudinal section. The sample holder is placed on the sample holder, the middle part of the sample holder is separated by absorbent paper, and the two surfaces of the sample holder are corrugated paper.
And thirdly, placing the thick and thin ends of the arranged plants on the water absorbing paper at the bottoms of the specimen holders and the corrugated paper pad in a mutually staggered manner, spreading branches and leaves, placing the front and back sides of the leaves, bending the overlong herbaceous or liana in a shape of 'N', 'V' or 'w', placing 2 or 3 pieces of dry water absorbing paper between every two specimens, placing 5-10 pieces of water absorbing paper and corrugated paper on the top, placing the other specimen holder on the specimen holder, and screwing the specimen holder to be the tightest by using triangular iron and screws. The traditional specimen holder is enlarged in equal proportion, the length of the specimen holder is 40cm, the width of the specimen holder is 35cm, the width of the batten is 4cm, the thickness of the batten is 2.5cm, the collected specimen is compressed in the specimen holder as far as possible, and the compression resistance of the specimen holder is improved. The binding band uses 0.3cm thick angle iron, diameter 1cm screw, can reduce 3 times with the sample clamp thickness of putting in order.
Fourthly, the pressed specimen folder is placed in a refrigerator with the temperature of 1-6 ℃ for refrigeration.
And fifthly, opening the specimen clamp the dried absorbent paper the next day, and rearranging the specimen. The specimen holder was again pressurized with screws and angle iron and placed in a refrigerator at 1-6 ℃ until the specimen was completely dried.
The shape and collection of the specimen pressed by the refrigeration pressurization method are consistent, the color is not changed, but the luster is slightly worse than that of the fresh and alive plants. The technical means disclosed in the invention scheme are not limited to the technical means disclosed in the above embodiments, but also include the technical scheme formed by any combination of the above technical features. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and such improvements and modifications are also considered to be within the scope of the present invention.
Claims (3)
1. A preparation method for manufacturing a wax leaf specimen by using a refrigeration pressurization method is characterized by comprising the following steps: the preparation method comprises the following steps:
firstly, storing fresh plants collected in the field in a heat preservation box at the temperature of 1-6 ℃;
secondly, arranging the collected plant specimens and then placing the plant specimens on a specimen holder, wherein the plant specimens are separated by absorbent paper, and corrugated paper is used between the plant specimens and the specimen holder;
expanding the size of a traditional specimen holder in equal proportion, wherein the size is that the holder is 40cm long, 35cm wide, 4cm wide and 2.5cm thick, and the collected specimen is compressed in the specimen holder; the binding belt is made of triangular iron with the thickness of 0.3cm and a screw with the diameter of 1 cm; the specimen holder is pressed by a screw and a triangular iron,
fourthly, storing the pressed specimen in a refrigerator at the temperature of 1-6 ℃;
and fifthly, opening the specimen holder for replacing dry absorbent paper, rearranging the specimen, applying pressure to the specimen holder by using screws and triangular iron again, and placing the specimen holder in a refrigerator at the temperature of 1-6 ℃ until the specimen is completely dried.
2. The method of claim 1, wherein the preparation of the wax leaf specimen by cold storage and pressurization comprises: the arrangement in the second step comprises the following steps: pruning dead leaves on fresh plants, standing the roots in water for a moment, and washing back and forth to remove mud; cutting the tuber or root into transverse section and longitudinal section.
3. The method of claim 1, wherein the preparation of the wax leaf specimen by cold storage and pressurization comprises: in the third step, the finished thick and thin ends of the plants are placed on the absorbent paper at the bottoms of the specimen holders and the corrugated paper pad in a mutually staggered manner, branches and leaves are spread out, the front and back sides of the leaves are placed, overlong herbaceous or liana plants are bent in the shapes of 'N', 'V' and 'w', 2 or 3 pieces of dry absorbent paper are placed among the plant specimens, 5-10 pieces of absorbent paper and corrugated paper are placed on the top, the other specimen holder is placed on the specimen holder, and the specimen holder is screwed to the top by triangular iron and screws.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010206742.5A CN111183976A (en) | 2020-03-23 | 2020-03-23 | Preparation method for preparing wax leaf specimen by using refrigeration pressurization method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010206742.5A CN111183976A (en) | 2020-03-23 | 2020-03-23 | Preparation method for preparing wax leaf specimen by using refrigeration pressurization method |
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| CN111183976A true CN111183976A (en) | 2020-05-22 |
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| CN202010206742.5A Pending CN111183976A (en) | 2020-03-23 | 2020-03-23 | Preparation method for preparing wax leaf specimen by using refrigeration pressurization method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112697556A (en) * | 2020-12-25 | 2021-04-23 | 四川省农业科学院园艺研究所 | Method for preparing citrus plant root tip chromosome slices |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972392A (en) * | 2012-12-20 | 2013-03-20 | 李嘉 | Low-temperature vacuum drying preservation method for flowers |
| CN103493801A (en) * | 2013-09-18 | 2014-01-08 | 安徽师范大学 | Method for preparing herbarium specimens |
| CN108925551A (en) * | 2018-07-09 | 2018-12-04 | 公安县中医医院 | A kind of method of microwave production plant specimen |
| CN110771601A (en) * | 2019-11-21 | 2020-02-11 | 钦州市中医药研究所 | Fresh plant specimen and preparation method thereof |
-
2020
- 2020-03-23 CN CN202010206742.5A patent/CN111183976A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972392A (en) * | 2012-12-20 | 2013-03-20 | 李嘉 | Low-temperature vacuum drying preservation method for flowers |
| CN103493801A (en) * | 2013-09-18 | 2014-01-08 | 安徽师范大学 | Method for preparing herbarium specimens |
| CN108925551A (en) * | 2018-07-09 | 2018-12-04 | 公安县中医医院 | A kind of method of microwave production plant specimen |
| CN110771601A (en) * | 2019-11-21 | 2020-02-11 | 钦州市中医药研究所 | Fresh plant specimen and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 施泽荣等: "《机场植物学野外实习指导》", 31 March 2017, 合肥工业大学出版社 * |
| 李钦: "《药用植物学》", 31 July 2012, 中国医药科技出版社 * |
| 陈学林: "《植物学实验实习指导》", 31 December 2016, 甘肃科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112697556A (en) * | 2020-12-25 | 2021-04-23 | 四川省农业科学院园艺研究所 | Method for preparing citrus plant root tip chromosome slices |
| CN112697556B (en) * | 2020-12-25 | 2024-02-23 | 四川省农业科学院园艺研究所 | Method for preparing citrus plant root tip chromosome tablet |
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Application publication date: 20200522 |
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