CN106327986A - Manufacturing method for early embryo presentation model of mouse - Google Patents
Manufacturing method for early embryo presentation model of mouse Download PDFInfo
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- CN106327986A CN106327986A CN201610944838.5A CN201610944838A CN106327986A CN 106327986 A CN106327986 A CN 106327986A CN 201610944838 A CN201610944838 A CN 201610944838A CN 106327986 A CN106327986 A CN 106327986A
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/36—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for zoology
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- General Health & Medical Sciences (AREA)
- Algebra (AREA)
- Computational Mathematics (AREA)
- Animal Behavior & Ethology (AREA)
- Mathematical Analysis (AREA)
- Health & Medical Sciences (AREA)
- Mathematical Physics (AREA)
- Zoology (AREA)
- Business, Economics & Management (AREA)
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Abstract
The invention belongs to the technical field of popular science display, specifically relates to a manufacturing method for early embryo presentation model of mouse. The method comprises the steps of fetching newly born mouse with 8.5 days of gestational age, putting the mouse in 4% of PFA for 24h, preparing 200ml and 1% of agar, pouring the agar in a glass dish after being heated and melted by a microwave oven, putting the mouse specimen in the agar in semi-solidified state, cutting the specimen with the agar by the blade after the agar is completely solidified, putting the specimen into a transparent glass specimen bottle, pouring 4% of PFA in the specimen bottle for long-term storage. The embryo model is prepared by living body fixing method, agar and 4% of PFA, and then the mouse embryo in different stages can be presented vividly and completely in the manner of real specimen.
Description
Technical field
The invention belongs to science popularization display technique field, specifically, relate to a kind of Mouse Embryos and show the making of model
Method.
Background technology
Along with the development of science and technology, popular science propaganda becomes more and more important.But life sciences basic research field is
One extremely complex field, microcosmic is mostly compared in involved test, it is impossible to system demonstration is shown.Mistake due to fetal development
Journey is in vivo, and the process observing fetal development wanting very intuitive and convenient at present is extremely difficult, the most well side of displaying
Formula helps our observational study animal full development process in vivo.
Similar popular science teaching aid uses mice or the mankind's (miscarriage) fetus, is fixed in formalin solution making exhibition
Representation model, the displaying model that the method makes is not fixed owing to growing natural law, it is impossible to follow the tracks of the full mistake showing that whole life is grown
Journey, it addition, do popular science teaching aid with mankind's aborted fetus have dispute of ethic, be unsuitable for as exhibition article and teaching aid towards child or
Adolescent student uses, is further improved.
Summary of the invention
It is an object of the invention to provide a kind of Mouse Embryos and show the manufacture method of model, and combined branch general education
Tool, towards teenager and the public, using mice as model, the overall process that system demonstration life is grown.
The embodiment provides a kind of Mouse Embryos and show the manufacture method of model, comprise the steps:
Take gestational age 8.5 days to newborn mice, be placed in 4%PFA at least 24 hours；
Making 200 milliliter of 1% agar, microwave-oven-heating is poured in glass dish after melting；
After being in half curdled appearance in agar, mice is put into the mice specimen fixed；
After agar solidifies completely, with blade, specimen UNICOM agar is together scaled off, put in clear glass Specimen bottle；
In Specimen bottle, fill 4%PFA preserve for a long time.
Further, glass dish is the glass culture dish of 20-30 cm diameter.
Further, a glass dish makes four mice specimen.
Further, each Specimen bottle is placed 1 mice specimen.
Compared with prior art the invention has the beneficial effects as follows: by the way of live body is fixing, use agar, 4%PFA system
Make embryo model, can be by the mice embryonic of different phase with the form of specimen (8 days-19 days) in kind, the most complete is presented in
People are at the moment.
Detailed description of the invention
The present invention is described in detail below, but it should explanation, these embodiments not limit to the present invention
System, those of ordinary skill in the art are according to the equivalent transformation in these embodiment institute work energy, method or structure or replace
In generation, within belonging to protection scope of the present invention.
Present embodiments provide a kind of Mouse Embryos and show the manufacture method of model, comprise the steps:
A) take gestational age 8.5 days to newborn mice, be placed in 4%PFA at least 24 hours；
B) prepare the glass culture dish of 20-30 cm diameter, clean up；
C) making 200 milliliter of 1% agar, microwave-oven-heating is poured in glass dish after melting；
Etc. d) mice is put in glass dish after being in half curdled appearance by agar, and a general ware can put four specimen；
E) after agar solidifies completely, with blade, specimen UNICOM agar is together scaled off, put into clear glass Specimen bottle
In；
F) finally in Specimen bottle, fill 4%PFA to preserve for a long time.
The embodiment that the present invention provides, in the way of live body is fixing, uses agar, and 4%PFA makes embryo model, can be by not
With the mice embryonic in stage with the form of specimen (8 days-19 days) in kind, the most complete is presented in people at the moment, visibility
Height, without ethical issues, it is possible to preserve for a long time, maintains the embryo's original form in parent and organizational structure as far as possible, knot
Close picture (being fertilized-8 days) and the overall process from sperm, ovum, fertilization process and early embryonic development can be shown.
The a series of detailed description of those listed above is only for the feasibility embodiment of the present invention specifically
Bright, they also are not used to limit the scope of the invention, all equivalent implementations made without departing from skill of the present invention spirit
Or change should be included within the scope of the present invention.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of the spirit or essential attributes of the present invention, it is possible to realize the present invention in other specific forms.Therefore, no matter
From the point of view of which point, all should regard embodiment as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all by fall in the implication of equivalency and scope of claim
Change is included in the present invention.
Claims (4)
1. the manufacture method of a Mouse Embryos displaying model, it is characterised in that comprise the steps:
Take gestational age 8.5 days to newborn mice, be placed in 4%PFA at least 24 hours；
Making 200 milliliter of 1% agar, microwave-oven-heating is poured in glass dish after melting；
After being in half curdled appearance in agar, mice is put in glass dish；
After agar solidifies completely, with blade, specimen UNICOM agar is together scaled off, put in clear glass Specimen bottle；
In Specimen bottle, fill 4%PFA preserve for a long time.
A kind of Mouse Embryos the most according to claim 1 shows the manufacture method of model, it is characterised in that described glass
Glass ware is the glass culture dish of 20-30 cm diameter.
A kind of Mouse Embryos the most according to claim 2 shows the manufacture method of model, it is characterised in that an institute
State four mice specimen of making in glass dish.
A kind of Mouse Embryos the most according to claim 3 shows the manufacture method of model, it is characterised in that Mei Gebiao
This bottle preserves 1 mice specimen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610944838.5A CN106327986A (en) | 2016-11-02 | 2016-11-02 | Manufacturing method for early embryo presentation model of mouse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610944838.5A CN106327986A (en) | 2016-11-02 | 2016-11-02 | Manufacturing method for early embryo presentation model of mouse |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106327986A true CN106327986A (en) | 2017-01-11 |
Family
ID=57818868
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610944838.5A Pending CN106327986A (en) | 2016-11-02 | 2016-11-02 | Manufacturing method for early embryo presentation model of mouse |
Country Status (1)
| Country | Link |
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| CN (1) | CN106327986A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108583115A (en) * | 2018-05-02 | 2018-09-28 | 阜阳师范学院 | A kind of manufacturing system of Mouse Embryos model |
| CN112652222A (en) * | 2020-04-30 | 2021-04-13 | 华南农业大学 | Intra-ovarian fertilization process of female silkworm moths and model display method of ovaries |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB901702A (en) * | 1958-07-25 | 1962-07-25 | Ichikawa Woollen Textile Co Lt | A water-absorbing porous substance for use in the paper making industry |
| JPH0374301A (en) * | 1989-08-16 | 1991-03-28 | Akio Imuro | Production of large-sized resin-embedded biological specimen |
| US20080175822A1 (en) * | 2007-01-19 | 2008-07-24 | Schatten Gerald P | Non-human primate embryonic stem and germ cells: methods of use and methods of making same |
| US20090241206A1 (en) * | 2008-03-04 | 2009-09-24 | University Of Iowa Research Foundation | Methods for cloning ferrets and transgenic ferret models for diseases |
| CN103583509A (en) * | 2013-11-27 | 2014-02-19 | 青岛海洋科技馆 | Making method of gel specimen |
-
2016
- 2016-11-02 CN CN201610944838.5A patent/CN106327986A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB901702A (en) * | 1958-07-25 | 1962-07-25 | Ichikawa Woollen Textile Co Lt | A water-absorbing porous substance for use in the paper making industry |
| JPH0374301A (en) * | 1989-08-16 | 1991-03-28 | Akio Imuro | Production of large-sized resin-embedded biological specimen |
| US20080175822A1 (en) * | 2007-01-19 | 2008-07-24 | Schatten Gerald P | Non-human primate embryonic stem and germ cells: methods of use and methods of making same |
| US20090241206A1 (en) * | 2008-03-04 | 2009-09-24 | University Of Iowa Research Foundation | Methods for cloning ferrets and transgenic ferret models for diseases |
| CN103583509A (en) * | 2013-11-27 | 2014-02-19 | 青岛海洋科技馆 | Making method of gel specimen |
Non-Patent Citations (4)
| Title |
|---|
| 曾晓蓓等: "制备组织石蜡切片琼脂预包埋法的应用性探讨", 《实验技术与管理》 * |
| 杜卓民主编: "《实用组织学技术》", 30 April 1998, 北京：人民卫生出版社 * |
| 许凯: "基因修饰大鼠模型的研究进展", 《生命科学》 * |
| 谷甜甜: "一个与小鼠胚胎发育相关新基因0610038D11Rik的研究", 《中国优秀博硕士学位论文全文数据库(硕士) 基础科学辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108583115A (en) * | 2018-05-02 | 2018-09-28 | 阜阳师范学院 | A kind of manufacturing system of Mouse Embryos model |
| CN108583115B (en) * | 2018-05-02 | 2021-06-18 | 阜阳师范学院 | Manufacturing system of mouse early embryo model |
| CN112652222A (en) * | 2020-04-30 | 2021-04-13 | 华南农业大学 | Intra-ovarian fertilization process of female silkworm moths and model display method of ovaries |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |
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| RJ01 | Rejection of invention patent application after publication |