CN1887961A - Small biological sample preparing process with transparant water soluble resin - Google Patents
Small biological sample preparing process with transparant water soluble resin Download PDFInfo
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- CN1887961A CN1887961A CNA2006100544947A CN200610054494A CN1887961A CN 1887961 A CN1887961 A CN 1887961A CN A2006100544947 A CNA2006100544947 A CN A2006100544947A CN 200610054494 A CN200610054494 A CN 200610054494A CN 1887961 A CN1887961 A CN 1887961A
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Abstract
The small biological sample of transparent water soluble resin is prepared with polyethylene glycol, transparent urea-formaldehyde resin and glacial acetic acid as coagulator in certain weight proportion. The preparation process includes the following three steps: preparing base glue, embedding biological sample and preparing facing glue. The preparation process is simple and low in cost, the prepared transparent resin has high flowability and high transparency and is suitable for embedding biological sample, and the prepared biological sample is tough, pest resistant, and mildewproof.
Description
One, technical field
The invention belongs to the biology techniques field, relate to a kind of method of making biological sample.
Two, background technology
At present, the method for making biological sample has following several: first kind is to adopt urea-formaldehyde resin embedding biological sample, and its advantage is that cost is low; But its shortcoming is a colloid water content height, and the evaporation owing to moisture is very easily ftractureed in making processes, does not have flexibility, frangible, poor water resistance.Second kind is to adopt dry process, but its defective is that sample subjects to damage by worms, go mouldy, and easy to change because of losing original color when ambient moisture is big.The third is to use the formaldehyde humid preparation, and this sample is not only harmful, and contaminate environment, and the time also can make sample lose original color after having grown in addition; The 4th kind is to add polyvinyl alcohol in urea-formaldehyde resin, though this method success ratio is higher than first kind, also fails to change fully and can change resin cracks, does not have flexible shortcoming.
Three, summary of the invention
The invention provides a kind of simple method of biological sample of making, overcome existing weak point of making in the biological sample, and be a kind of making method of energy prolonged preservation sample.The sample of this method preparation is not easy to crack, the tool flexibility, and transparency is good and can keep original appearance.
1, related raw material comprises as follows in the small biological sample preparing process with transparant water soluble resin method: transparent urea-formaldehyde resin liquid, polyoxyethylene glycol and Glacial acetic acid.Wherein, transparent urea-formaldehyde resin liquid and polyoxyethylene glycol weight ratio are 1: 0.4～2.3, form transparent water-soluble resin (annotate: the molecular weight of above-mentioned polyoxyethylene glycol is between 200～600) after reacting.The add-on of Glacial acetic acid (content 〉=99.5%) be polyoxyethylene glycol and transparent urea-formaldehyde resin carry out transparent glue total amount that polyreaction forms 5～15%, led peptizer effect.Comprise following operation with above-mentioned transparent water-soluble resin specimen embedding: the preparation of a. primer:, put into 50～55 ℃ of incubators and solidify with the bottom of this resin injection mould; B. specimen embedding: on the primer after the biological sample after will handling is placed on and solidifies, inject this resin, just its submergence sample, and insert in 50～55 ℃ of incubators and solidify; C. the making of face glue: this resin is injected glue face after the b operation is solidified, place 50～55 ℃ of incubators to solidify after, from mould, take out, be placed on 50～55 ℃ of incubators once more until completely solidified; After treating its completely solidified, take out from incubator, preparation of specimen finishes.
Remarks: the thickness that injects transparent water-soluble resin in the a.b.c. operation is respectively 10～20mm; For each procedure of a.b.c., sample need be placed in 50～55 ℃ of incubators and solidify, and (look Glacial acetic acid add-on what) setting time be about 24h, just can carry out next process.
The transparent water-soluble resin that the present invention generates with polyoxyethylene glycol and the reaction of transparent urea-formaldehyde resin, has the stronger toughness different with other resin, under the big situation of temperature variation, do not ftracture, indeformable, add in the urea-formaldehyde resin at polyoxyethylene glycol, increase along with add-on, flexibility does not ftracture equally along with increasing yet, and is indeformable.
Advantage of the present invention: the method that the present invention relates to is a kind of simple method, and this method raw material is easy to get, and cost is low; The transparent water-soluble resin of preparation is mobile strong, and transparency is good, is convenient to the embedding of biological sample; The sample tool toughness of this method preparation, not easy to crack, the energy insect protected, mildew-resistant, and can keep the original color of sample, be the making method of the higher small-sized biological sample of a kind of success ratio.
Four, embodiment
Embodiment one:
First procedure is 600 polyoxyethylene glycol with adding molecular weight in the transparent urea-formaldehyde resin glue, and its weight proportion is 1: 1, and through fully stirring, polyreaction forms transparent water-soluble resin liquid.Second operation work, add peptizer Glacial acetic acid (content 〉=99.5%) make resin accelerated solidification, its add-on be polyoxyethylene glycol and transparent urea-formaldehyde resin carry out polyreaction formation transparent water-soluble resin liquid gross weight 10%; (produce owing to have a large amount of bubbles in stirring, through fully stirring so with vacuum pump 2～5min) the no a large amount of bubbles generations to this glue of bleeding; After placing for some time, when treating that this glue surface does not have the bubble generation again, promptly can be used for making biological sample.Three process: its concrete specimen embedding step is as follows:
A. this resin is injected in the mould and make primer, thickness is 10～20mm, is positioned in 50～55 ℃ of incubators to solidify through 24h;
B. the embedding biological sample is put into the biological sample after handling on the primer face, injects this resin, makes this resin submergence sample, is positioned in 50～55 ℃ of incubators and solidifies through 24h;
C. make face glue, the glue face after this resin injection b operation is solidified, thickness is 10～20mm, is positioned in 50～55 ℃ of incubators to solidify through 24h.From mould, take out, put back in 50～55 ℃ of incubators and solidify.When taking out the sample of making after the completely solidified in this incubator, specimen embedding is finished.
Embodiment two:
First procedure is 400 polyoxyethylene glycol with adding molecular weight in the transparent urea-formaldehyde resin glue, and its weight proportion is 1: 2.3, and through fully stirring, polyreaction forms transparent water-soluble resin liquid.Second operation work, add peptizer Glacial acetic acid (content 〉=99.5%) and make the resin accelerated solidification, its add-on is that polyoxyethylene glycol and transparent urea-formaldehyde resin carry out polyreaction and form 5% of transparent water-soluble resin liquid gross weight, (produce through fully stirring owing to have a large amount of bubbles in stirring, so with vacuum pump bleed 1～3min) to this glue no a large amount of bubbles, place for some time, when treating that this glue surface does not have the bubble generation again, promptly can be used for making biological sample.Operation is with embodiment one, a.b.c later on.
Embodiment three:
First procedure is 200 polyoxyethylene glycol with adding molecular weight in the transparent urea-formaldehyde resin glue, its weight proportion be 1: 0.7 through fully stirring, polyreaction forms transparent water-soluble resin liquid.Second operation work, add peptizer Glacial acetic acid (content 〉=99.5%) and make the resin accelerated solidification, its add-on is that polyoxyethylene glycol and transparent urea-formaldehyde resin carry out polyreaction and form 13% of transparent water-soluble resin liquid gross weight, through fully stir (produce owing to have a large amount of bubbles in stirring, thus with vacuum pump bleed 1～3min) to this glue no a large amount of bubbles; Place for some time, when treating that this glue surface does not have the bubble generation again, promptly can be used for making biological sample.Operation is with embodiment one, a.b.c later on.
Claims (2)
1, the preparation method of the small-sized biological sample liquid of a kind of transparent water-soluble resin, it is characterized in that: raw materials used is polyoxyethylene glycol, transparent urea-formaldehyde resin (liquid) and peptizer Glacial acetic acid, transparent urea-formaldehyde resin liquid and polyoxyethylene glycol weight ratio are 1: 0.4～2.3, form transparent water-soluble resin through polyreaction; The add-on of peptizer Glacial acetic acid (content 〉=99.5%) be polyoxyethylene glycol and transparent urea-formaldehyde resin (liquid) carry out transparent water-soluble resin glue gross weight that polyreaction forms 5～15%, in order to the accelerated solidification time; The molecular weight of described polyoxyethylene glycol is between 200～600.
2, a kind of transparent water-soluble resin embedding biological sample method is characterized in that: the described transparent water-soluble resin of claim 1 is made biological sample comprise following operation:
The preparation of a, primer: require the transparent water-soluble resin of 1 preparation to inject in the mould aforesaid right and make primer, be positioned in 50～55 ℃ of incubators and solidify through 24h;
B, embedding biological sample: the biological sample after will handling is put on the primer face, injects this resin, makes this resin submergence sample, is positioned in 50～55 ℃ of incubators to solidify through 24h;
The preparation of c, face glue: this resin is injected glue face after the b operation is solidified, be positioned in 50～55 ℃ of incubators and solidify through 24h.From mould, take out, put back in 50～55 ℃ of incubators and solidify.When taking out the sample of making after the completely solidified in this incubator, specimen embedding completes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100544947A CN100569854C (en) | 2006-07-28 | 2006-07-28 | Small biological sample preparing process with transparant water soluble resin |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100544947A CN100569854C (en) | 2006-07-28 | 2006-07-28 | Small biological sample preparing process with transparant water soluble resin |
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| CN1887961A true CN1887961A (en) | 2007-01-03 |
| CN100569854C CN100569854C (en) | 2009-12-16 |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101935447A (en) * | 2010-08-12 | 2011-01-05 | 重庆师范大学 | Transparent water soluble resin |
| CN102301994A (en) * | 2011-08-03 | 2012-01-04 | 刘卉秋 | Standard sample of dodder, and preparation method thereof |
| CN102323125A (en) * | 2011-08-03 | 2012-01-18 | 刘卉秋 | Euphorbia dentata Michx standard sample and preparation method thereof |
| CN102353568A (en) * | 2011-08-03 | 2012-02-15 | 胡强 | Trogoderma granarium standard sample and preparation method thereof |
| CN103299985A (en) * | 2013-05-21 | 2013-09-18 | 西北农林科技大学 | Cantharias specimen and making method thereof |
| CN106982819A (en) * | 2017-05-10 | 2017-07-28 | 广西柳州中嘉知识产权服务有限公司 | Honeybee Slide processing |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
| CN108207929A (en) * | 2018-02-06 | 2018-06-29 | 国家海洋局北海环境监测中心 | A kind of store method of zoobenthos sample |
| CN108877446A (en) * | 2018-07-09 | 2018-11-23 | 张家港市德仁科教仪器设备有限公司 | A kind of foot collagen position embedding method and model |
| CN110802778A (en) * | 2019-11-08 | 2020-02-18 | 深圳集美殿堂实业有限公司 | Forming process for embedding large special-shaped objects in transparent resin |
| CN112277521A (en) * | 2020-10-27 | 2021-01-29 | 安庆师范大学 | Method for making creative microorganism specimen picture with stable appearance character |
| CN113180033A (en) * | 2021-04-28 | 2021-07-30 | 黄倞朗 | Preparation method of small biological specimen |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB953342A (en) * | 1959-05-27 | 1964-03-25 | Minnesota Mining & Mfg | Improved resin compositions and their production |
-
2006
- 2006-07-28 CN CNB2006100544947A patent/CN100569854C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101935447A (en) * | 2010-08-12 | 2011-01-05 | 重庆师范大学 | Transparent water soluble resin |
| CN101935447B (en) * | 2010-08-12 | 2012-09-26 | 重庆师范大学 | Transparent water soluble resin |
| CN102301994A (en) * | 2011-08-03 | 2012-01-04 | 刘卉秋 | Standard sample of dodder, and preparation method thereof |
| CN102323125A (en) * | 2011-08-03 | 2012-01-18 | 刘卉秋 | Euphorbia dentata Michx standard sample and preparation method thereof |
| CN102353568A (en) * | 2011-08-03 | 2012-02-15 | 胡强 | Trogoderma granarium standard sample and preparation method thereof |
| CN103299985A (en) * | 2013-05-21 | 2013-09-18 | 西北农林科技大学 | Cantharias specimen and making method thereof |
| CN106982819A (en) * | 2017-05-10 | 2017-07-28 | 广西柳州中嘉知识产权服务有限公司 | Honeybee Slide processing |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
| CN108207929A (en) * | 2018-02-06 | 2018-06-29 | 国家海洋局北海环境监测中心 | A kind of store method of zoobenthos sample |
| CN108877446A (en) * | 2018-07-09 | 2018-11-23 | 张家港市德仁科教仪器设备有限公司 | A kind of foot collagen position embedding method and model |
| CN110802778A (en) * | 2019-11-08 | 2020-02-18 | 深圳集美殿堂实业有限公司 | Forming process for embedding large special-shaped objects in transparent resin |
| CN112277521A (en) * | 2020-10-27 | 2021-01-29 | 安庆师范大学 | Method for making creative microorganism specimen picture with stable appearance character |
| CN113180033A (en) * | 2021-04-28 | 2021-07-30 | 黄倞朗 | Preparation method of small biological specimen |
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| Publication number | Publication date |
|---|---|
| CN100569854C (en) | 2009-12-16 |
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