CN113142183A - System and method for manufacturing ecological specimens of verruca virginiana - Google Patents
System and method for manufacturing ecological specimens of verruca virginiana Download PDFInfo
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- CN113142183A CN113142183A CN202110202615.2A CN202110202615A CN113142183A CN 113142183 A CN113142183 A CN 113142183A CN 202110202615 A CN202110202615 A CN 202110202615A CN 113142183 A CN113142183 A CN 113142183A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
Abstract
The invention discloses a system for manufacturing an ecological verruca vulgaris specimen, which belongs to the technical field of biological specimen manufacturing and comprises a culture device (1), an air breather and a dosing device, wherein the air breather comprises an air pump (2) and an air pipe (3), and the dosing device comprises a medicine storage tank, a flow rate adjusting part and a dosing pipe; one (5) end of the flow rate adjusting part is communicated with the medicine storage tank, and the other end of the flow rate adjusting part is communicated with the medicine feeding pipe; the air outlet of the breather pipe (3) and the outlet of the medicine feeding pipe are arranged at the bottom of the culture device (1); the invention also discloses a method for preparing the ecological sample of the verruca virginiana by using the system. The system and the method for manufacturing the ecological specimen of the green wart sea anemone can quickly manufacture and obtain the vivid ecological specimen of the green wart sea anemone.
Description
Technical Field
The invention belongs to the technical field of biological specimen preparation, relates to a device and a method for preparing a marine biological ecological specimen, and particularly relates to a system and a method for preparing an anemone viridis ecological specimen.
Background
The specimen is an animal, plant, mineral and other real object, can be preserved for a long time after various treatments, and can keep the original appearance as much as possible, thereby being used for exhibition, demonstration, education, identification, examination and other various researches; the processing process of the specimen is an important factor for determining the quality of the specimen, so that the selection of a proper specimen preparation mode is an important precondition for preparing a high-quality specimen. In the preparation of aquatic animal specimens, anesthesia of coelenterates in particular can lead the specimens to be stretched more ideally, thereby achieving the best preparation effect.
The verruca virginiana is marine coelenterate, and in a natural state, the tentacles of the verruca virginiana stretch to be opened and live like a sunflower. Most coelenterates have no skeleton supporting system, and are stimulated by the external environment, the tentacles and the cylinders of the green wart anemones are very easy to shrink, so that in order to obtain lifelike specimens, the specimens are firstly anesthetized, and a large amount of time is spent waiting for the tentacles of the green wart anemones to stretch before the anesthetization. At present, the anesthetic is usually added into the solution in portions to perform anesthetic treatment on the verruca vulgaris, on one hand, tentacles originally stretched by the verruca vulgaris are easy to contract again under the stimulation of the anesthetic, and on the other hand, the time required for anesthetizing the verruca vulgaris is long, and generally takes 4-6 hours. Therefore, a system and a method for rapidly manufacturing and obtaining vivid green wart sea anemone ecological specimen are urgently needed.
Disclosure of Invention
The invention aims to overcome the problem that in the process of manufacturing the ecological sample of the anemone viridans, the vivid ecological sample of the anemone viridans is difficult to quickly prepare due to the stress response of the anemone viridans to the change of the external environment in the prior art, and provides a system and a method capable of quickly manufacturing and obtaining the vivid ecological sample of the anemone viridans.
In order to solve the technical problem, the invention provides a system for manufacturing an ecological verruca vulgaris specimen, which comprises a culture device, a ventilation device and a dosing device, wherein the ventilation device comprises an air pump and an air pipe, and the dosing device comprises a medicine storage tank, a flow rate adjusting part and a dosing pipe; 1-5 sets of medicine adding devices are arranged; one end of the flow rate regulating component is communicated with the medicine storage tank, and the other end of the flow rate regulating component is communicated with the medicine feeding pipe; the culture device is arranged as a groove body, the position of the medicine storage tank is higher than that of the groove body, the flow speed adjusting part is internally provided with an adjusting valve for changing the diameter of the water flow of the pipe cavity, and the air outlet of the vent pipe and the outlet of the medicine feeding pipe are arranged at the bottom of the groove body of the culture device.
On one hand, the ventilating device continuously blows air into the culture seawater, the air generates bubbles in the water through the ventilating pipe, sea waves in the natural environment are simulated, and the time for waiting for the verruca viridis tentacles to stretch out is greatly shortened; on the other hand, the air is pressed into the water by the air-breather, so that the oxygen in the air is fully contacted with the water, thereby achieving the purpose that partial oxygen is dissolved into the water, increasing the oxygen content of the water and meeting the requirement of the verruca viridis on the oxygen.
The medicine adding device is provided with 1-5 sets. In specific operations, different drugs, such as various anesthetics, and formalin solutions of killed verruca virginiana can be placed into different dosing devices according to specific situations.
As a further improvement measure of the invention, in the system for manufacturing the ecological verruca vulgaris specimen, the flow velocity adjusting part comprises a tube body, an adjusting valve and a sealing ring, the tube body is arranged into a cylindrical body, one end of the tube body is communicated with the medicine storage tank, the other end of the tube body is communicated with the medicine feeding tube, the side wall of the cylindrical body is provided with 1-8 through holes which are radially communicated, the adjusting valve is respectively arranged in the through holes, the sealing ring is arranged at the joint of the two ends of the adjusting valve and the tube wall, the adjusting valve is respectively provided with a first flow guide hole and a second flow guide hole, the first flow guide hole and the second flow guide hole are arranged on the adjusting valve in a cross shape, the diameters of the first flow guide holes are the same, the diameters of the second flow guide holes are gradually reduced in sequence, one of the first flow guide holes can be arranged as a valve switch, so that when the medicine is fed into the culture device, only the adjusting valves with different diameters are rotated according to the demand of the medicine feeding amount, the flow guide holes with different apertures are communicated, so that the flow acceleration of different medicines can be controlled, and the use is convenient.
When the verruca viridis specimen is manufactured, the reaction of the verruca viridis to the medicament feeding speed is observed, the medicament feeding speed is adjusted through the flow speed adjusting part, the medicament feeding time is shortened as much as possible on the premise that the manufacturing effect of the verruca viridis ecological specimen is influenced by the tentacle and cylinder shrinkage caused by the stress reaction of medicament stimulation of the verruca viridis, and the manufacturing efficiency of the specimen is improved.
As a further improvement measure of the invention, the air outlet of the vent pipe and the outlet of the dosing pipe are arranged side by side in a fitting manner, and the air outlet of the vent pipe is provided with an air stone. The gas of the gas outlet of breather pipe is bloated, reaches the wave among the simulation natural environment and to the effect of aquatic oxygenation on the one hand, and on the other hand can make the medicament flow into breed the marine aquatic after quick with breed seawater misce bene, local solution concentration is too high when avoiding the medicament to add, and green wart sea anemone produces stress response and leads to tentacle and cylinder shrink, influences the preparation effect of green wart sea anemone ecological sample.
The invention also provides a method for manufacturing the green wart sea anemone ecological specimen by using the system for manufacturing the green wart sea anemone ecological specimen, which comprises the following steps:
(1) adding seawater into the culture device;
(2) immersing live verruca virginiana in the seawater in the step (1) for culturing;
(3) starting an air pump, introducing air into seawater, and allowing the verruca virginiana tentacles to be completely opened;
(4) anaesthetizing sea anemone green warts: preparing an anesthetic, adding the anesthetic into the culture device through a dosing device, and controlling the feeding speed of the anesthetic through a flow speed adjusting part;
(5) killing malva virginiana: preparing a formalin solution, adding the formalin solution into a culture device through a dosing device, controlling the feeding speed of the formalin solution through a flow speed regulating part, continuously introducing air into seawater for 1-2 hours after the feeding of the formalin solution is finished, and closing an air pump;
(6) and (3) preservation of an ecological sample of verruca virescens: and (4) taking the verruca virginiana treated in the step (5) out, and soaking in 5-8% formalin solution for preservation.
After the anesthetic is fed into the dosing device in the step (4), the dosing device can be used for feeding formalin solution in the step (5). The number of the medicine adding devices can be set according to the number of the types of the medicines, and each medicine is respectively used by one medicine adding device.
As a further improvement of the invention, the seawater has a salinity of 26.6-34.04% and a pH value of 7-8.5.
The seawater can be natural seawater or artificially prepared by seawater salt. The sea water with too high or too low salinity and too high or too low pH value can cause the death of the verruca virginiana.
As a further improvement measure of the invention, the weight of the verruca virginiana in the step (2) is 6.0g-15.0 g.
The invention adopts a method of adding anesthetic and formalin solution into culture seawater to prepare the ecological sample of the anemone viridis, and the quality of the anemone viridis is positively correlated with the concentration of the anesthetic and the formalin solution. Under normal conditions, the weight of each verruca viridis is 6.0g-15.0 g. If the sea anemone is too large in mass, a good anesthetic effect cannot be achieved rapidly when the anesthetic is added in a feeding mode, the sea anemone cannot be killed rapidly when the formalin solution is added in a feeding mode, and the sea anemone can be killed rapidly only by directly injecting the formalin solution into the sea anemone body by using an injector when the sea anemone with large mass is killed.
As a further improvement measure of the invention, the ventilation amount of the air pump to each liter of seawater in the step (3) is 2-5L/min. Under the condition of the ventilation intensity, after several minutes, the verruca virginiana tentacles can stretch open.
As a further improvement measure of the invention, when 6.0g to 15.0g of verruca virginiana is selected to prepare a specimen, the anesthetic in the step (4) is selected from a magnesium sulfate solution with the concentration of 12.57 percent to 31.20 percent, and the dosage of the magnesium sulfate solution in per liter of seawater is 75ml to 150 ml; the feeding time of the magnesium sulfate solution is 60-100 min.
If the dosage of the anesthetic is too small, the anemonia viridans cannot be completely anesthetized, and if the dosage of the anesthetic is too large, the body cavity of the anemonia viridans is everted; the feeding time of the anesthetic is controlled to be 60-100min, so that the situation that the stress reaction of the verruca virginiana caused by the sudden increase of the concentration of the anesthetic in seawater and the influence on the manufacturing effect of the ecological specimen are avoided.
As a further improvement measure of the invention, when 6.0g-15.0g of green wart sea anemone is selected to be used for preparing the specimen, the concentration of the formalin solution in the step (5) is 5-8%, and the dosage of the formalin solution in each liter of seawater is 50-75 ml; the feeding time of the formalin solution is 60-70 min. At the moment, if the concentration of the formalin solution used for killing the verruca viridis is lower than 5%, the effect of killing the verruca viridis cannot be achieved, and if the concentration of the formalin solution is higher than 8%, the verruca viridis can be stimulated to generate stress reaction, so that tentacles and cylinders shrink, and the manufacturing effect of the ecological specimen of the verruca viridis is influenced; the feeding time of the formalin solution is controlled to be 60-70min, so that the situation that the stress reaction of the verruca virginiana caused by the sudden increase of the concentration of the formalin solution in seawater and the influence on the manufacturing effect of the ecological specimen are avoided.
As a further improvement measure of the invention, in the step (5), after the air pump is turned off, the method further comprises the step of continuously soaking the killed verruca virginiana in the seawater in the step (5) for 12-48 hours.
When the verruca viridis killed, if the verruca viridis is directly taken out and soaked in 5-8% formalin solution for storage, on one hand, the concentration difference of solute concentration inside and outside the verruca viridis cell is large, so that the verruca viridis cell is dehydrated and contracted, and the effect of the verruca viridis ecological specimen is influenced; on the other hand, at the moment, the verruca virginiana is probably not killed, if the verruca virginiana is directly taken out and soaked in a 5-8% formalin solution, the stress response of the verruca virginiana and tentacle contraction can be caused due to sudden increase of the concentration of the formalin solution, and the effect of the ecological specimen of the verruca virginiana is influenced; if the verruca viridis is continuously soaked in the seawater in the step (5) for 12-48 hours after the verruca viridis is killed, the phenomenon can be effectively avoided.
Compared with the prior art, the invention has the beneficial effects that: 1. according to the invention, the aeration device is used for blowing air into the culture seawater, so that on one hand, sea waves can be simulated, dissolved oxygen in water can be increased, and the time for waiting for the tentacles of the verruca virginiana to stretch out is shortened; on the other hand, the stirring effect is achieved, so that the fed-in agent is immediately and uniformly mixed with seawater when entering the culture seawater, and the problem that the concentration of the agent in the culture seawater is suddenly increased to cause stress reaction of the verruca virginiana, which causes tentacles and cylinder shrinkage, and influences the manufacturing effect of the ecological specimens of the verruca virginiana is avoided; 2. according to the invention, the flow rate of the medicament is regulated by the flow rate regulating component, so that the time of medicament flow addition is shortened as much as possible and the preparation efficiency of the specimen is improved on the premise of avoiding the influence on the preparation effect of the ecological specimen of the anoplophora viridans due to the contraction of tentacles and cylinders caused by the stress reaction of the increase and stimulation of the medicament concentration; 3. the invention provides the corresponding relation between the quality of the verruca viridis and the dosage and the feeding time of the medicament, provides convenience for production practice and experimental operation, reduces the time for groping the dosage and the feeding time of the medicament during the preparation of the ecological verruca viridis specimen, and improves the specimen preparation efficiency.
Drawings
Fig. 1 is a schematic view of an ecological preparation system for verruca virginiana according to embodiment 1 of the present invention.
Fig. 2 is a front view of a flow rate regulating section in embodiment 1 of the present invention.
Fig. 3 is a sectional view taken along line a-a in fig. 2.
Fig. 4 is a sectional view taken along line B-B in fig. 2.
Fig. 5 is a schematic view of the regulator valve 12 of the present invention.
Fig. 6 shows an ecological sample of verruca virginiana prepared in example 2 of the present invention.
FIG. 7 shows an ecological sample of Semiaquilegia adoxoides prepared in example 3 of the present invention.
FIG. 8 shows an ecological sample of Semiaquilegia adoxoides prepared in example 4 of the present invention.
FIG. 9 shows an ecological sample of Semiaquilegia adoxoides prepared in example 5 of the present invention.
FIG. 10 shows an ecological sample of Semiaquilegia adoxoides prepared in example 6 of the present invention.
FIG. 11 shows an ecological sample of Semiaquilegia adoxoides prepared in example 7 of the present invention.
The reference numbers illustrate: 1-a culture device, 2-an air pump, 3-an air pipe, 4-a medicine storage tank I, 5-a flow rate adjusting part I, 6-a medicine feeding pipe I, 7-a medicine storage tank II, 8-a flow rate adjusting part II, 9-a medicine feeding pipe II, 10-air stone, 11-a pipe body, 12-an adjusting valve, 13-a sealing ring, 14-a diversion hole I and 15-a diversion hole II.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
On the contrary, the invention is intended to cover alternatives, modifications, equivalents and alternatives which may be included within the spirit and scope of the invention as defined by the appended claims. Furthermore, in the following detailed description of the present invention, certain specific details are set forth in order to provide a better understanding of the present invention. It will be apparent to one skilled in the art that the present invention may be practiced without these specific details.
The normal temperature of the invention means that the temperature is 20-30 ℃.
Example 1: the system for manufacturing the ecological verruca viridis specimen comprises a culture device 1, a ventilation device, a first dosing device and a second dosing device, wherein the ventilation device comprises an air pump 2 and a ventilation pipe 3, the first dosing device comprises a first medicine storage tank 4, a first flow rate regulating part 5 and a first dosing pipe 6, one end of the first flow rate regulating part 5 is communicated with the first medicine storage tank 4, and the other end of the first flow rate regulating part is communicated with the first dosing pipe 6; the second dosing device comprises a second medicine storage tank 7, a second flow rate adjusting part 8 and a second dosing pipe 9, wherein one end of the second flow rate adjusting part 8 is communicated with the second medicine storage tank 7, and the other end of the second flow rate adjusting part is communicated with the second dosing pipe 9; the air outlet of the air pipe 3 and the outlets of the first dosing pipe 6 and the second dosing pipe 9 are attached to the bottom of the culture device 1 side by side; the bottom of the dosing device is higher than the level of the seawater surface in the culture device 1, and the medicament flows into the culture seawater under the action of gravity; the gas outlet of breather pipe 3 is provided with air stone 10, has a lot of micropores above air stone 10, and air stone 10 is put into the water, utilizes ventilation unit to blast air, and gas passes through breather pipe 3 and gets into air stone 10, produces even micropore bubble in aqueous, makes the wave of simulation more press close to nature like this, and verruca virginiana tentacle touches the hand and changes and extend.
As shown in fig. 2 to 5, the flow rate adjusting part includes a tube 11, an adjusting valve 12 and a sealing ring 13, the tube 11 is a tubular body, one end of the tube 11 is communicated with the drug storage tank, the other end of the tube 11 is communicated with the drug feeding pipe, the sidewall of the tubular body is provided with 6 first through holes which are through in the radial direction, the adjusting valve 12 is respectively arranged in the first through holes, the sealing ring 13 is arranged at the connection between the two ends of the adjusting valve 12 and the tube wall, the adjusting valve 12 is respectively provided with a first diversion hole 14 and a second diversion hole 15, the first diversion hole 14 and the second diversion hole 15 are arranged in a cross shape on the adjusting valve 12, the diameters of the first diversion holes 14 are the same, the diameters of the second diversion holes 15 are gradually reduced in sequence, the diameter can be set to be between 0.1 mm and 2 mm, one of the first diversion holes 14 can be set as a valve switch, only one diversion hole 14 is arranged, and plays a role of conducting when the first diversion hole 14 is opened, when the first diversion hole 14 is closed, the first diversion hole plays a role in blocking water flow. Therefore, when the medicine is added into the culture device 1, according to the requirement of the medicine adding amount, the valve switch is opened, the regulating valves 12 with different apertures are rotated, the second flow guide holes 15 with different apertures are switched on, the flow adding speed of different medicines can be controlled, the use is convenient, and the medicine adding amount is accurate.
Example 2: the method for manufacturing the verruca vulgaris by using the system for manufacturing the ecological verruca vulgaris specimen comprises the following steps: selecting a beaker with the volume of 1500ml as a culture device 1, adding 800ml of seawater with the salinity of 26.2 percent and the pH value of 7 into the beaker, wherein the temperature of the seawater is normal temperature; selecting fresh and alive verruca virginiana with the mass of 6.0g and placing the fresh verruca virginiana in a beaker; starting the air pump 2, wherein the ventilation volume is 2L/min, simulating sea waves, and completely opening the tentacles of the sea anemones 35min later; preparing a 12.57% magnesium sulfate solution as an anesthetic in advance, adding 60ml of the 12.57% magnesium sulfate solution into a first medicine storage tank 4, adjusting a first flow rate adjusting part 5, and adding the magnesium sulfate solution into the culture device 1 through a first medicine adding pipe 6 at a constant flow rate for 60 min; standing for 60min, and touching with glass rod to make sure that the contact hand does not contract, and the green wart anemone is completely anesthetized; preparing 8% formalin solution in advance, adding 50ml of 8% formalin solution into a second medicine storage tank 7, adjusting a second flow rate adjusting part 8 to enable the formalin solution to flow into the culture device 1 through a second medicine adding pipe 9 at a constant speed, wherein the flowing time is 70min, after the flowing of the formalin solution is completed, continuously introducing air into the seawater for 1 hour, and closing the air pump 2; and (3) continuously standing and soaking the malva virginiana in the beaker for 12 hours, taking out the malva virginiana, soaking the malva virginiana in 8% formalin solution for preservation, and taking a picture, wherein the picture is shown in figure 6.
Example 3: the method for manufacturing the verruca vulgaris by using the system for manufacturing the ecological verruca vulgaris specimen comprises the following steps: selecting a beaker with the volume of 1500ml as a culture device 1, adding 800ml of seawater with the salinity of 26.2 percent and the pH value of 8.5 into the beaker, wherein the temperature of the seawater is normal temperature; selecting fresh and alive verruca virginiana with the mass of 10g and placing the fresh verruca virginiana in a beaker; starting the air pump 2, wherein the ventilation volume is 3L/min, simulating sea waves, and completely opening the tentacles of the sea anemones after 29 min; preparing 31.20% magnesium sulfate solution as anesthetic in advance, adding 80ml of 31.20% magnesium sulfate solution into a first medicine storage tank 4, adjusting a first flow rate adjusting part 5, and adding the magnesium sulfate solution into the culture device 1 through a first medicine adding pipe 6 at a constant flow rate for 80 min; standing for 80min, and touching with glass rod to make sure that the semiaquilegia virens is not contracted and completely anesthetized; preparing 6% formalin solution in advance, adding 48ml of 8% formalin solution into a second medicine storage tank 7, adjusting a second flow rate adjusting part 8 to enable the formalin solution to flow into the culture device 1 through a second medicine adding pipe 9 at a constant speed, wherein the flowing time is 65min, after the flowing of the formalin solution is completed, continuously introducing air into the seawater for 2 hours, and closing the air pump 2; and (3) continuously standing and soaking the malva virginiana in the beaker for 24 hours, taking out the malva virginiana, soaking the malva virginiana in a 6% formalin solution for preservation, and taking a picture, wherein the picture is shown in figure 7.
Example 4: the method for manufacturing the verruca vulgaris by using the system for manufacturing the ecological verruca vulgaris specimen comprises the following steps: selecting a beaker with the volume of 1500ml as a culture device 1, adding 800ml of seawater with the salinity of 34.04 percent and the pH value of 7.5 into the beaker, wherein the temperature of the seawater is normal temperature; selecting fresh and alive verruca virginiana with the mass of 15g and placing the fresh and alive verruca virginiana in a beaker; starting the air pump 2, wherein the ventilation capacity is 4L/min, simulating sea waves, and completely opening the tentacles of the sea anemones after 25 min; preparing a magnesium sulfate solution with the concentration of 20.80% in advance as an anesthetic, adding 120ml of the magnesium sulfate solution with the concentration of 20.80% into a first medicine storage tank 4, adjusting a first flow rate adjusting part 5, and enabling the magnesium sulfate solution to flow into a culture device 1 through a first medicine adding pipe 6 at a constant speed for 100 min; standing for 100min, and touching with glass rod to make sure the Sedum viridans is not contracted and completely anesthetized; preparing 8% formalin solution, adding 60ml of 8% formalin solution into a second medicine storage tank 7, adjusting a second flow rate adjusting part 8 to enable the formalin solution to be fed into the culture device 1 through a second medicine feeding pipe 9 at a constant flow rate, wherein the feeding time is 70min, after the feeding of the formalin solution is finished, continuously introducing air into the seawater for 1.5 hours, and closing the air pump 2; and (3) continuously standing and soaking the malva virginiana in the beaker for 48 hours, taking out the malva virginiana, soaking the malva virginiana in 8% formalin solution for preservation, and taking a picture, wherein the picture is shown in figure 8.
Example 5: the method for manufacturing the verruca vulgaris by using the system for manufacturing the ecological verruca vulgaris specimen comprises the following steps: selecting a beaker with the volume of 1500ml as a culture device 1, adding 800ml of seawater with the salinity of 26.2 percent and the pH value of 7.5 into the beaker, wherein the temperature of the seawater is normal temperature; selecting fresh and alive verruca virginiana with the mass of 15g and placing the fresh and alive verruca virginiana in a beaker; starting the air pump 2, wherein the ventilation volume is 4L/min, simulating sea waves, and completely opening the tentacles of the sea anemones after 17 min; preparing a 12.57% magnesium sulfate solution as an anesthetic in advance, adding 50ml of the 12.57% magnesium sulfate solution into a first medicine storage tank 4, adjusting a first flow rate adjusting part 5, and adding the magnesium sulfate solution into the culture device 1 through a first medicine adding pipe 6 at a constant flow rate for 24 min; after 22min, the verruca vulgaris was lightly touched by a glass rod, and when the verruca vulgaris was touched, the hand was significantly contracted, and the verruca vulgaris was not completely anesthetized; preparing 8% formalin solution in advance, adding 60ml of 8% formalin solution into a second medicine storage tank 7, adjusting a second flow rate adjusting part 8, and enabling the formalin solution to flow into the culture device 1 through a second medicine feeding pipe 9 at a constant speed for 70 min; and (3) continuously standing and soaking the malva virginiana in the beaker for 48 hours, taking out the malva virginiana, soaking the malva virginiana in 8% formalin solution for preservation, and taking a picture, wherein the picture is shown in figure 9.
Example 6: the method for manufacturing the verruca vulgaris by using the system for manufacturing the ecological verruca vulgaris specimen comprises the following steps: selecting a beaker with the volume of 1500ml as a culture device 1, adding 800ml of seawater with the salinity of 26.2 percent and the pH value of 8.5 into the beaker, wherein the temperature of the seawater is normal temperature; selecting fresh and alive verruca virginiana with the mass of 10g and placing the fresh verruca virginiana in a beaker; starting the air pump 2, wherein the ventilation volume is 5L/min, simulating sea waves, and completely opening the tentacles of the sea anemones 17min later; preparing 31.20% magnesium sulfate solution as anesthetic in advance, adding 80ml of 31.20% magnesium sulfate solution into a first medicine storage tank 4, adjusting a first flow rate adjusting part 5, and adding the magnesium sulfate solution into the culture device 1 through a first medicine adding pipe 6 at a constant flow rate for 80 min; standing for 80min, and touching with glass rod to make sure that the semiaquilegia virens is not contracted and completely anesthetized; preparing 6% formalin solution in advance, adding 48ml of 8% formalin solution into a second medicine storage tank 7, adjusting a second flow rate adjusting part 8, and enabling the formalin solution to be added into the culture device 1 through a second medicine adding pipe 9 at a constant flow rate for 30min, wherein the flow time is poor because the flow acceleration of the formalin solution is too high when the anemonia viridans is killed, the anemonia viridis tentacles are contracted due to sudden increase of the concentration of the formalin solution in seawater, and the preparation effect of the specimen is poor; and (3) continuously standing and soaking the malva virginiana in the beaker for 24 hours, taking out the malva virginiana, soaking the malva virginiana in a 6% formalin solution for preservation, and taking a picture, wherein the picture is shown in figure 10.
Example 7: the method for manufacturing the verruca vulgaris by using the system for manufacturing the ecological verruca vulgaris specimen comprises the following steps: selecting a beaker with the volume of 1500ml as a culture device 1, adding 800ml of seawater with the salinity of 26.2 percent and the pH value of 7 into the beaker, wherein the temperature of the seawater is normal temperature; selecting fresh and alive verruca virginiana with the mass of 6.0g and placing the fresh verruca virginiana in a beaker; starting the air pump 2, wherein the ventilation volume is 2L/min, simulating sea waves, and completely opening the tentacles of the sea anemones after 34 min; preparing a 12.57% magnesium sulfate solution as an anesthetic in advance, adding 60ml of the 12.57% magnesium sulfate solution into a first medicine storage tank 4, adjusting a first flow rate adjusting part 5, and adding the magnesium sulfate solution into the culture device 1 through a first medicine adding pipe 6 at a constant flow rate for 60 min; standing for 60min, and touching with glass rod to make sure the semiaquilegia virens not contract and completely narcotize the semiaquilegia virens; preparing 8% formalin solution in advance, adding 50ml of 8% formalin solution into a second medicine storage tank 7, adjusting a second flow rate adjusting part 8, and enabling the formalin solution to flow into the culture device 1 through a second medicine adding pipe 9 at a constant speed for 70 min; immediately taking out the verruca viridis, soaking the verruca viridis in 8% formalin solution for storage, wherein the verruca viridis is not killed, the stress reaction of the verruca viridis and tentacle contraction are caused by sudden increase of the concentration of the formalin solution, the ecological specimen of the verruca viridis is poor in preparation effect, and the photograph is taken as shown in fig. 11.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and it will be apparent to those skilled in the art that several modifications and improvements may be made without departing from the present invention, and these should be construed as falling within the scope of the present invention.
Claims (10)
1. A preparing system of an ecological verruca vulgaris specimen comprises a culture device (1), an aeration device and a dosing device, and is characterized in that the aeration device comprises an air pump (2) and an aeration pipe (3), and the dosing device comprises a medicine storage tank, a flow rate adjusting part and a dosing pipe; the medicine adding device is set to be 1-5 sets; one (5) end of the flow rate adjusting part is communicated with the medicine storage tank, and the other end of the flow rate adjusting part is communicated with the medicine feeding pipe; the culture device (1) is arranged as a groove body, the position of the medicine storage tank is higher than that of the groove body, a regulating valve (12) used for changing the diameter of the water flow of the pipe cavity is arranged in the flow rate regulating part, and the gas outlet of the ventilating pipe (3) and the outlet of the medicine adding pipe are arranged at the bottom of the groove body of the culture device (1).
2. The system for preparing an ecological sample of verruca vulgaris according to claim 1, the flow velocity adjusting part comprises a tube body (11), an adjusting valve (12) and a sealing ring (13), the tube body (11) is arranged into a cylindrical body, one end of the tube body (11) is communicated with the medicine storage tank, the other end of the tube body (11) is communicated with the medicine feeding pipe, 1 to 8 through holes I which are penetrated in the radial direction are arranged on the side wall of the cylindrical body, regulating valves (12) are respectively arranged in the through holes I, sealing rings (13) are arranged at the joints of the two ends of the regulating valve (12) and the pipe wall, a first diversion hole (14) and a second diversion hole (15) are respectively arranged on the regulating valve (12), the first diversion hole (14) and the second diversion hole (15) are arranged on the regulating valve (12) in a cross shape, the diameters of the first diversion hole (14) are the same, and the diameters of the second diversion hole (15) are gradually reduced in sequence.
3. The system for preparing the ecological verruca vulgaris specimen according to claim 1, wherein an air outlet of the vent pipe (3) and an outlet of the medicine feeding pipe are attached side by side, and an air stone (10) is arranged at the air outlet of the vent pipe (3).
4. The method for preparing the ecological sample of the verruca viridis by using the ecological sample preparation system of the verruca viridis as claimed in claim 2, which is characterized by comprising the following steps:
(1) adding seawater into the culture device (1);
(2) immersing live verruca virginiana in the seawater in the step (1) for culturing;
(3) starting the air pump (2) until the verruca virginiana tentacles are completely opened;
(4) anaesthetizing sea anemone green warts: the anesthetic is prepared, the anesthetic is added into the culture device (1) through a dosing device, and the flow rate of the anesthetic is controlled through a flow rate adjusting part;
(5) killing malva virginiana: preparing a formalin solution, adding the formalin solution into the culture device (1) through a dosing device, controlling the feeding speed of the formalin solution through a flow speed regulating part, continuously introducing air into the seawater for 1-2 hours after the feeding of the formalin solution is finished, and closing an air pump (2);
(6) and (3) preservation of an ecological sample of verruca virescens: and (4) taking the verruca virginiana treated in the step (5) out, and soaking in 5-8% formalin solution for preservation.
5. The method for preparing the ecological sample of verruca virginiana according to claim 4, wherein the seawater in the step (1) has a salinity of 26.6% -34.04% and a pH value of 7-8.5.
6. The method for preparing the ecological sample of the verruca nigra as claimed in claim 4, wherein the weight of the verruca nigra in the step (2) is 6.0g to 15.0 g.
7. The method for preparing the ecological sample of verruca aeruginosa according to claim 4 wherein the air pump (2) in step (3) has a ventilation volume of 2-5L/min per liter of seawater.
8. The method for preparing an ecological sample of anemonia viridescens as claimed in claim 6, wherein in step (4), said anesthetic is selected from magnesium sulfate solution with concentration of 12.57% -31.20%, and the dosage of magnesium sulfate solution per liter of seawater is 75-150 ml; the feeding time of the magnesium sulfate solution is 60-100 min.
9. The method for preparing an ecological sample of verruca viridis as claimed in claim 6, wherein the concentration of the formalin solution in the step (5) is 5-8%, and the amount of the formalin solution in each liter of seawater is 50-75 ml; the feeding time of the formalin solution is 60-70 min.
10. The method for preparing the ecological sample of the verruca virginiana according to claim 4, wherein in the step (5), the killed verruca virginiana is continuously soaked in the seawater in the step (5) for 12-48 hours after the air pump (2) is turned off.
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| FR2857946A1 (en) * | 2003-07-24 | 2005-01-28 | Amblard Overseas Trading | Living aquatic animals e.g. coral, transporting package, for aquarium, has living aquatic animals covered by plastic humidifier sheet for being protected from dehydration by contact with air and partially immersed in water |
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