CN103348967B - Semen diluting solution for artificial insemination of poultry and application method thereof - Google Patents
Semen diluting solution for artificial insemination of poultry and application method thereof Download PDFInfo
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- CN103348967B CN103348967B CN201310295107.9A CN201310295107A CN103348967B CN 103348967 B CN103348967 B CN 103348967B CN 201310295107 A CN201310295107 A CN 201310295107A CN 103348967 B CN103348967 B CN 103348967B
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Abstract
The invention discloses a semen diluting solution for artificial insemination of poultry and an application method thereof. 100 mL of the semen diluting solution contains 2.2-2.8 g of sodium glutamate, 1.2-1.6 g of fructose, 0.8-1.2 g of sodium citrate, 10.1-0.3 g of KCl, 0.2-0.4 g of magnesium sulfate and 80,000-100,000 IU of penicillins. The semen diluting solution is capable of improving the extracorporeal viability of sperms and further increasing the fertility rate of hatching-eggs.
Description
Technical field
The invention belongs to duck technology of artificial insemination field, be specifically related to a kind of for the test-tube semen diluent of poultry and using method thereof.
Background technology
Half kind of duck is the filial generation of public kind duck and married woman's parent's home duck, and because kind duck is the duck kind not belonging to together with a duck, the rate of fertilization of natural crossing is lower, and average rate of fertilization is only 30% ~ 40%.In half kind of duck produces, adopt public kind duck and married woman's parent's home duck to carry out artificial insemination at present, rate of fertilization 30% ~ 40% during by natural crossing brings up to 70 ~ 80%, has been widely applied in actual production.
The quality of semen quality has determined the test-tube success or failure of poultry to a great extent, and the rate of fertilization of kind of egg is played to vital effect.On business fowl lacks sexual accessory gland, nutrition content in seminal fluid is little, the deposit of the endogenous energy of sperm itself is also very limited, therefore in the very short time, semen quality will reduce greatly in vitro, this has just limited the sperm survival time in vitro to a great extent, therefore when carrying out poultry artificial insemination and using undiluted seminal fluid, general requirement seminal fluid is finished in 30 minutes after extraction, this has brought certain difficulty just to test-tube work, has limited the utilization of good breeding male fowl on time and space.
In order to improve the utilization ratio of breeding male fowl and the rate of fertilization of kind egg, it is very common way that seminal fluid is diluted.The object of semen dilution is mainly to expand semen volume, reduces in semen collection process because seminal fluid is bonded at and on semen collection cup, causes unnecessary waste; Meanwhile, also can by semen diluent to supplementing energy material in seminal fluid and by semen diluent and in seminal fluid because of the objectionable impuritiess such as the movable lactic acid producing of sperm metabolism, the survival time of prolongation sperm.Good semen diluent should possess the desirable living environment of sperm, and neutralization or eliminating are to the dysgenic factor of sperm.In addition, semen diluent also should have draw materials easily, configuration is simple, cost is low, be convenient to the features such as popularization.In recent ten years, the research of kind duck semen diluent increases gradually, but not yet has so far a comparatively desirable diluent formula.
Summary of the invention
The object of the present invention is to provide one for the test-tube semen diluent of poultry and using method thereof, semen diluent of the present invention can improve sperm viability in vitro, further improves the rate of fertilization of kind of egg.
For achieving the above object, the present invention adopts following technical scheme:
A kind of for the test-tube semen diluent of poultry, in every 100 mL semen diluents, contain Sodium Glutamate 2.2-2.8g, fructose 1.2-1.6g, Trisodium Citrate 0.8-1.2g, KCl 0.1-0.3g, magnesium sulfate 0.2-0.4g, penicillin 80,000-100,000 IU.
Using method for the test-tube semen diluent of poultry as above, in beaker, add Sodium Glutamate, fructose, Trisodium Citrate, KCl, magnesium sulfate and penicillin by proportioning, adding distil water to 100 mL again, stir with glass stick, after dissolving completely, move in the volumetric flask of 100mL, adding distil water to scale shakes up, and regulating pH value is 6.5 ~ 7.5.
Formula Glutamic Acid sodium has better protecting effect to sperm, and motility of sperm is had to desirable influence; The metabolism that fructose is seminal fluid provides necessary energy; Trisodium Citrate has neutralized as weak organic salt the lactic acid that sperm produces because of vital movement in vitro, regulates the pH value of diluent; A small amount of K
+that to maintain the normal function of sperm necessary; Mg
2+there is the motion of protection sperm and the feature of prolongation sperm life; Penicillin has the effect of bactericidal antiphlogistic, can reduce the infection to female bird reproductive tract in semen deposition process.
Remarkable advantage of the present invention is: semen diluent of the present invention can improve sperm viability in vitro, further improves the rate of fertilization of kind of egg.
Embodiment
(1) composition of diluent
Mainly comprise Sodium Glutamate, fructose, Trisodium Citrate, Repone K, magnesium sulfate, penicillin element and distilled water.
(2) preparation of diluent
When preparation, should be noted following item:
all apparatus that configuration diluent uses, thoroughly washes clean, after sterilization, can be used;
it is fresh that diluent need keep, preferably now with the current, as condition does not allow, can in 4 DEG C of refrigerators, deposit 1 week;
it is neutral that distilled water pH used should be;
the medicine that prepared and diluted liquid is used, composition is pure, weighs and wants accurately, dissolves and wants fully, to carry out disinfection after filtration after sealing;
the interpolation of penicillin, must, in the time that diluent is cooled to room temperature, accurately add by consumption; 6. diluent is placed on after configuring in the water-bath of 60-70 DEG C and carries out water-bath sterilization, and the temperature of water-bath is too high, and because containing fructose in diluent, excess Temperature fructose can decompose, and affects the quality of diluent.
(3) using method of diluent
after semen collection, seminal fluid should dilute immediately, and lower, lopsided, the dead sperm count of seminal fluid longer its motility rate storage period is more;
diluent must be adjusted consistent with the temperature of seminal fluid, before using, need to be placed in the constant water bath box of 42 DEG C, 10min left and right, the temperature of diluent and temperature that seminal fluid is stored in duck body are close, in order to avoid dilution when seminal fluid because diluent temperature is too low, cause sperm to be subject to cold strike and dormancy or death, the rate of fertilization of impact kind of egg;
the Dilution ratio of diluent and seminal fluid is 1:1 or 1:2, and in actual production, the ratios that adopt 1:1 are diluted more;
the dilution of seminal fluid must be even, the diluent of the half that is used for diluting can be put in advance to collection essence cup, after adopting seminal fluid, rinse the seminal fluid on the smart wall of cup of collection with remaining half diluent again, in the time of dilution, need slowly to add along the smart wall of cup of collection, prevent diluent to pour into rapidly in seminal fluid, cause local dilution to hit;
after dilution, will collect essence cup and rotate gently, seminal fluid is mixed with diluent, must guard against concuss.
Diluent its rate of fertilization experimental basis of filling a prescription that the present invention proposes is, utilizes kind duck to produce half kind of duck with changing in vain duck hybridization, and test is chosen 2000 and is in that raising in cages of egg-laying peak is white changes duck, is divided at random 5 groups, and every group has 2 repetitions.Semen deposition time unification is selected 8:00-11:00 in the morning, collects altogether the kind egg of 4 days.Result shows that the average rate of fertilization of former seminal fluid is 76.59 ± 1.04%; Physiological saline is pressed 1:1 dilution proportion, and average rate of fertilization is 75.18 ± 0.63%; Physiological saline is pressed 1:2 dilution proportion, and average rate of fertilization is 73.49 ± 1.89%; Use diluent of the present invention to press 1:1 dilution proportion, average fertilization rate reached is to 79.25 ± 1.70%; Use diluent of the present invention to press 1:2 dilution proportion, average fertilization rate reached is to 76.97 ± 1.53%; Show to utilize this diluent to kind duck seminal fluid according to the dilution proportion of 1:1,1:2, the average rate of fertilization of kind of egg is all higher than the rate of fertilization of former seminal fluid and physiological saline 1:1,1:2 dilution proportion, when particularly this diluent is pressed the dilution proportion of 1:1, plant the fertilization rate reached of egg to 79.25%, be significantly higher than physiological saline group, also higher nearly 3 percentage points than former seminal fluid, can be applicable aborning.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. for the test-tube semen diluent of poultry, it is characterized in that: in 100 mL semen diluents, contain Sodium Glutamate 2.2-2.8g, fructose 1.2-1.6g, Trisodium Citrate 0.8-1.2g, KCl 0.1-0.3g, magnesium sulfate 0.2-0.4g, penicillin 80,000-100,000 IU; Described semen diluent is a kind duck semen diluent, for the production of half kind of duck.
2. the preparation method for the test-tube semen diluent of poultry as claimed in claim 1, it is characterized in that: in beaker, add Sodium Glutamate, fructose, Trisodium Citrate, KCl, magnesium sulfate and penicillin by proportioning, adding distil water to 100 mL again, stir with glass stick, after dissolving completely, move in the volumetric flask of 100mL, adding distil water to scale shakes up, and regulating pH value is 6.5 ~ 7.5.
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| CN201310295107.9A CN103348967B (en) | 2013-07-15 | 2013-07-15 | Semen diluting solution for artificial insemination of poultry and application method thereof |
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| CN201310295107.9A CN103348967B (en) | 2013-07-15 | 2013-07-15 | Semen diluting solution for artificial insemination of poultry and application method thereof |
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| CN103348967A CN103348967A (en) | 2013-10-16 |
| CN103348967B true CN103348967B (en) | 2014-10-08 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609552A (en) * | 2013-12-06 | 2014-03-05 | 安徽五星食品股份有限公司 | Poultry semen diluent for maintaining sperm motility and prolonging insemination time |
| CN107913118A (en) * | 2017-12-19 | 2018-04-17 | 佛山科学技术学院 | A kind of mule duck artificial insemination method |
| CN110973120A (en) * | 2019-12-25 | 2020-04-10 | 西北农林科技大学 | Preparation method of Muscovy duck semen normal temperature preservation diluent |
| CN110946132A (en) * | 2019-12-31 | 2020-04-03 | 赵章成 | Poultry semen nutrient, nutrient diluent, preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070162A (en) * | 2013-01-30 | 2013-05-01 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103070162A (en) * | 2013-01-30 | 2013-05-01 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
Non-Patent Citations (4)
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| 曹金元等.稀释精液人工授精技术在罗曼粉蛋种鸡中的应用.《安徽农业科学》.2013,第41卷(第15期),第6740页表1，第1栏第2段，第6741页第1栏第2段. |
| 稀释精液人工授精技术在罗曼粉蛋种鸡中的应用;曹金元等;《安徽农业科学》;20130531;第41卷(第15期);第6740页表1，第1栏第2段，第6741页第1栏第2段 * |
| 辛清武等.鸭人工授精技术研究进展.《中国家禽》.2012,第34卷(第24期),第48页第1栏第1-2段. |
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Application publication date: 20131016 Assignee: Guisheng Chicken and Duck Breeding Professional Cooperative in Yanping District, Nanping City Assignor: INSTITUTE OF ANIMAL HUSBANDRY AND VETERINARY, FUJIAN ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023350000031 Denomination of invention: A semen diluent for artificial insemination of poultry and its use method Granted publication date: 20141008 License type: Common License Record date: 20230223 |
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