CN104396941B - A kind of solution for dilution of cock semen and preparation method thereof - Google Patents
A kind of solution for dilution of cock semen and preparation method thereof Download PDFInfo
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- CN104396941B CN104396941B CN201410621015.XA CN201410621015A CN104396941B CN 104396941 B CN104396941 B CN 104396941B CN 201410621015 A CN201410621015 A CN 201410621015A CN 104396941 B CN104396941 B CN 104396941B
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- 210000000582 semen Anatomy 0.000 title claims abstract description 64
- 238000010790 dilution Methods 0.000 title claims abstract description 58
- 239000012895 dilution Substances 0.000 title claims abstract description 58
- 239000000243 solution Substances 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 17
- VYLVYHXQOHJDJL-UHFFFAOYSA-K cerium trichloride Chemical compound Cl[Ce](Cl)Cl VYLVYHXQOHJDJL-UHFFFAOYSA-K 0.000 claims abstract description 16
- 239000002904 solvent Substances 0.000 claims abstract description 16
- BDKLKNJTMLIAFE-UHFFFAOYSA-N 2-(3-fluorophenyl)-1,3-oxazole-4-carbaldehyde Chemical compound FC1=CC=CC(C=2OC=C(C=O)N=2)=C1 BDKLKNJTMLIAFE-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229930091371 Fructose Natural products 0.000 claims abstract description 15
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims abstract description 15
- 239000005715 Fructose Substances 0.000 claims abstract description 15
- -1 compound vitamin Chemical class 0.000 claims abstract description 15
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims abstract description 15
- 229940050906 magnesium chloride hexahydrate Drugs 0.000 claims abstract description 15
- DHRRIBDTHFBPNG-UHFFFAOYSA-L magnesium dichloride hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[Cl-].[Cl-] DHRRIBDTHFBPNG-UHFFFAOYSA-L 0.000 claims abstract description 15
- 235000013923 monosodium glutamate Nutrition 0.000 claims abstract description 15
- 239000001508 potassium citrate Substances 0.000 claims abstract description 15
- 229960002635 potassium citrate Drugs 0.000 claims abstract description 15
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 claims abstract description 15
- 235000011082 potassium citrates Nutrition 0.000 claims abstract description 15
- 235000017281 sodium acetate Nutrition 0.000 claims abstract description 15
- 229940087562 sodium acetate trihydrate Drugs 0.000 claims abstract description 15
- 229940073490 sodium glutamate Drugs 0.000 claims abstract description 15
- 235000013343 vitamin Nutrition 0.000 claims abstract description 15
- 229940088594 vitamin Drugs 0.000 claims abstract description 15
- 229930003231 vitamin Natural products 0.000 claims abstract description 15
- 239000011782 vitamin Substances 0.000 claims abstract description 15
- 239000003085 diluting agent Substances 0.000 claims abstract description 14
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 230000003204 osmotic effect Effects 0.000 claims abstract description 8
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims description 16
- 229930182555 Penicillin Natural products 0.000 claims description 8
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 8
- 229940049954 penicillin Drugs 0.000 claims description 8
- 229960005322 streptomycin Drugs 0.000 claims description 8
- 229960002737 fructose Drugs 0.000 claims description 7
- 230000004720 fertilization Effects 0.000 abstract description 5
- 230000004899 motility Effects 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 208000007893 Salpingitis Diseases 0.000 abstract description 3
- 241000287828 Gallus gallus Species 0.000 description 8
- 230000009027 insemination Effects 0.000 description 7
- 230000002159 abnormal effect Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 230000008021 deposition Effects 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of solution for dilution of cock semen, with sterile purified water for solvent, every 1000ml diluent consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, compound vitamin 3g, cerium chloride 0.0024 ~ 0.0246mg, all the other be solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.Compared with existing solution for dilution of cock semen, the solution for dilution of cock semen of the present invention has: (1) improves motility of sperm；(2) Sperm survival time is effectively extended；(3) utilization rate of stock cock is significantly improved；(4) reduce Breeding hens artificial fertilization's process produce stress, the problem such as salpingitis tubal.
Description
Technical field
The present invention relates to a kind of diluent, especially relate to a kind of solution for dilution of cock semen and preparation method thereof.
Background technology
Along with the universal of cage poultry and development, the artificial insemination of chicken is widely popularized.The technology of artificial insemination difficulty of chicken is little, it is easy to grasp, and equipment is simple, small investment.The artificial insemination of chicken can reduce the number of animals raised of stock cock, and during natural mating, the male and female ratio of general chicken is 10-15 only left and right, and when adopting artificial insemination, as by inseminating weekly once, then 1 cock can join 100 even more hens.For ensureing the high rate of fertilization of hatching egg in production practices, general many employings semen deposition in 3-5 days is once.Calculating by this, during artificial insemination, the male and female ratio of chicken mostly is about 1:50.Than adopting natural mating method, can reduce by the cock number of animals raised of 80%, reduce hatching egg cost.When adopting artificial insemination, male and female chicken is sub-cage rearing.Sub-cage rearing, can provide convenient for breeding work, also provide condition for cock is individually supplemented the nutrients, be conducive to improving kind of a Chicken Semen quality.During employing artificial fertilization, cock all adopts single cage to raise under normal circumstances, thus the bucket of pecking reduced between chicken, reduces death rate.
The realization of above-mentioned superiority mainly depends on the design of good semen diluent, screening and use.The dilution of Chicken Semen is by the diluted of particular formulation.The freezen protective of Chicken Semen is still in experimental stage.To actual production, with the fresh more convenient practicality of seminal fluid semen deposition, therefore at this, preservation of Chicken Semen is not described with dilution process.Even if it should be noted that employing fresh semen semen deposition, after Chicken Semen extraction cock is external, if ambient temperature is too low also can affect its rate of fertilization.So, when ambient temperature is lower than 20 DEG C, it is desirable to adopt insulation collection essence cup collection essence.Thermos cup irrigates the warm water of 32 DEG C, if without special insulation collection essence cup during practical operation, it is also possible to additive method, to collection essence cup insulation, such as by teat glass collection essence, is subsequently placed in the vessel filling warm water.Excellent semen diluent should possess the desirable living environment of sperm, neutralizes or gets rid of the dysgenic factor of sperm.Additionally, semen diluent also should have draw materials easily, configuration is simple, cost is low, be easy to the features such as popularization.Although the research of solution for dilution of cock semen increases gradually at present, but not yet has an ideal diluent formula so far.
Summary of the invention
The technical problem to be solved is to provide a kind of solution for dilution of cock semen that can make motility of sperm raising, prolongation Sperm survival time.
To be solved by this invention another technical problem is that a kind of method preparing above-mentioned solution for dilution of cock semen of raising.
The technical problem to be solved is achieved by the following technical programs:
A kind of solution for dilution of cock semen, with sterile purified water for solvent, every 1000ml diluent consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, compound vitamin 3g, cerium chloride 0.0024 ~ 0.0246mg, all the other be solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Preferably, described cerium chloride is 0.0036mg.
Preferably, penicillin 400,000 IU, streptomycin 400,000 IU are also included.
A kind of method preparing arbitrary above-mentioned solution for dilution of cock semen, it is characterized in that, aseptically, weigh the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, compound vitamin to containing, in a small amount of sterile purified water sterile beaker, dissolving, add cerium chloride and/or penicillin and streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and subpackage is placed at 3 DEG C and keeps standby.
There is advantages that, compared with existing solution for dilution of cock semen, the solution for dilution of cock semen of the present invention has: (1) improves motility of sperm；(2) Sperm survival time is effectively extended；(3) utilization rate of stock cock is significantly improved；(4) reduce Breeding hens artificial fertilization's process produce stress, the problem such as salpingitis tubal.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be described in detail, and embodiment is only the preferred embodiment of the present invention, is not limitation of the invention.
Embodiment 1
A kind of solution for dilution of cock semen, with sterile purified water for solvent, every 1000ml diluent consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, compound vitamin 3g, cerium chloride 0.0024mg, all the other be solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
The method preparing above-mentioned solution for dilution of cock semen, aseptically, weigh the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, compound vitamin to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and subpackage is placed at 3 DEG C and keeps standby.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein Chicken Semen is 1:2 with the mass ratio of solution for dilution of cock semen.
Embodiment 2
A kind of solution for dilution of cock semen, with sterile purified water for solvent, every 1000ml diluent consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, compound vitamin 3g, cerium chloride 0.0036mg, all the other be solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
The method preparing above-mentioned solution for dilution of cock semen, aseptically, weigh the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, compound vitamin to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and subpackage is placed at 3 DEG C and keeps standby.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein Chicken Semen is 1:2 with the mass ratio of solution for dilution of cock semen.
Embodiment 3
A kind of solution for dilution of cock semen, with sterile purified water for solvent, every 1000ml diluent consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, compound vitamin 3g, cerium chloride 0.0246mg, all the other be solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
The method preparing above-mentioned solution for dilution of cock semen, aseptically, weigh the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, compound vitamin to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and subpackage is placed at 3 DEG C and keeps standby.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein Chicken Semen is 1:2 with the mass ratio of solution for dilution of cock semen.
Embodiment 4
A kind of solution for dilution of cock semen, with sterile purified water for solvent, every 1000ml diluent consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, compound vitamin 3g, cerium chloride 0.0036mg, penicillin 400,000 IU, streptomycin 400,000 IU, all the other be solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
The method preparing above-mentioned solution for dilution of cock semen, aseptically, weigh the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, compound vitamin to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, penicillin, streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and subpackage is placed at 3 DEG C and keeps standby.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein Chicken Semen is 1:2 with the mass ratio of solution for dilution of cock semen.
Comparative example 1
A kind of solution for dilution of cock semen, with sterile purified water for solvent, every 1000ml diluent consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, compound vitamin 3g, penicillin 400,000 IU, streptomycin 400,000 IU, all the other be solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
The method preparing above-mentioned solution for dilution of cock semen, aseptically, weigh the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, compound vitamin to containing in a small amount of sterile purified water sterile beaker, dissolve, add penicillin, streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and subpackage is placed at 3 DEG C and keeps standby.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein Chicken Semen is 1:2 with the mass ratio of solution for dilution of cock semen.
Observing the motility of sperm of embodiment 1 ~ 4, comparative example 1 in 0 ~ 72 hour, and measured the motility rate of sperm and abnormal rate every 24 hours, result is in Table 1.
The impact on chicken sperm motility rate of the table 1 different diluent cryopreservation seminal fluid
As it can be seen from table 1 embodiment 1 ~ 4 is compared with comparative example 1, after adding cerium chloride, the survival rate of sperm is obviously prolonged, and the abnormal rate of sperm substantially reduces；When the cerium chloride adding 0.0036mg, the survival rate of sperm is better, and abnormal rate is relatively low.
Solution for dilution of cock semen provided by the invention, owing to the vigor of sperm extends so that twice Inseminated time interval extends, thus reducing the amount of labour, saves labour cost.The utilization rate of stock cock can be provided simultaneously, reduce artificial insemination stress and the generation of salpingitis tubal, it is provided that the laying rate of Breeding hens.
Claims (3)
1. a solution for dilution of cock semen, it is characterized in that, with sterile purified water for solvent, every 1000ml diluent consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, compound vitamin 3g, cerium chloride 0.0036mg, all the other be solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
2. solution for dilution of cock semen according to claim 1, it is characterised in that also include penicillin 400,000 IU and streptomycin 400,000 IU.
3. the method preparing the arbitrary described solution for dilution of cock semen of claim 1 to 2, it is characterized in that, aseptically, the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, compound vitamin are weighed to containing, in a small amount of sterile purified water sterile beaker, dissolving, add cerium chloride, and it is optionally added the combination of penicillin and streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and subpackage is placed at 3 DEG C and keeps standby.
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| CN201410621015.XA CN104396941B (en) | 2014-11-07 | 2014-11-07 | A kind of solution for dilution of cock semen and preparation method thereof |
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| CN201410621015.XA CN104396941B (en) | 2014-11-07 | 2014-11-07 | A kind of solution for dilution of cock semen and preparation method thereof |
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| CN104396941A CN104396941A (en) | 2015-03-11 |
| CN104396941B true CN104396941B (en) | 2016-07-06 |
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| CN111406738A (en) * | 2020-05-09 | 2020-07-14 | 华南农业大学 | Chicken essence diluent for artificial oviduct insemination and preparation method thereof |
| CN113940340B (en) * | 2021-10-28 | 2022-05-17 | 广西富凤农牧集团有限公司 | Chicken sperm diluent and preparation method and application thereof |
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| US7083909B2 (en) * | 2002-03-14 | 2006-08-01 | Wisconsin Alumni Research Foundation | Composition containing gamete or embryo and animal white yolk and the use thereof |
| CN103609552A (en) * | 2013-12-06 | 2014-03-05 | 安徽五星食品股份有限公司 | Poultry semen diluent for maintaining sperm motility and prolonging insemination time |
| CN103704205B (en) * | 2014-01-15 | 2015-09-23 | 山东大学 | A kind of ox sperm freezing dilution liquid containing trace rare-earth and application thereof |
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Effective date of registration: 20181228 Address after: 529200 No. 10 Changxing Road, Taicheng, Taishan City, Jiangmen City, Guangdong Province (Living Building B) Patentee after: Taishan Secco Agricultural Technology Co., Ltd. Address before: 516898 No. 55 Xilin Road, Longmen County, Huizhou City, Guangdong Province Patentee before: Zhang Yingqin |