CN104322485A - Boar frozen semen diluted powder - Google Patents
Boar frozen semen diluted powder Download PDFInfo
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- CN104322485A CN104322485A CN201410529108.XA CN201410529108A CN104322485A CN 104322485 A CN104322485 A CN 104322485A CN 201410529108 A CN201410529108 A CN 201410529108A CN 104322485 A CN104322485 A CN 104322485A
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Abstract
The invention discloses a boar frozen semen diluted powder, which is characterized in that: the diluted powder is composed of D-glucose, trisodium citrate, EDTA sodium salt, potassium dihydrogen phosphate, potassium cyanide, streptomycin sulphate, polyvinyl alcohol (PVP.Typell), trishydroxymethylaminomethanol (Tris), citric acid, cysteine, OEP (amino-sodium dodecyl sulfate), SDS (sodium dodecyl sulfate), PGF2a, vitamin E, GSH (glutathione), hyaluronic acid, and N-acetyl-D-aminoglucose. The boar frozen semen diluted powder provided by the invention improves the fertilization rate and litter size of boar frozen semen, simultaneously can improve the product safety, reduces the risk of semen infection caused by animal derived components in the diluent, and fills the domestic blank in the field, thus having far-reaching significance.
Description
Technical field
The invention belongs to the freezen protective technical field of pig semen, specifically, relate to a boar frozen semen dilution powder.
Background technology
In recent years, the research emphasis of swine artificial insemination is transferred on frozen semen gradually.Pig frozen semen technology refers to and utilizes dry ice (-79 DEG C), liquid nitrogen (-196 DEG C) etc. to make cooling source, by freezing after seminal fluid specially treated, under the state being kept at ultralow temperature (-79 DEG C or-196 DEG C), reach long-term object of preserving, and after thawing, semen deposition reaches the technology of higher conception rate.China first raises pigs and pork big producing country in the world; especially in recent years; the pig industry of China raises the transition of mode to scale intensive farm mode scattered from tradition is extensive, the Artificial Insemination Technology of Swine of taking as the leading factor with normal temperature liquid storage seminal fluid technology by large-area promotion and application in Swine Production practice.But in the Swine Production of China, the work carried out for the research and apply of frozen semen is also short of very much, therefore, carry out the research and apply of technique in a deep going way, not only significant for the advantage playing further technology of artificial insemination, and for carrying out the genetic improvement of boar and for the aspect such as preservation of some peculiar local pig resources in imminent danger, also there is very important strategic importance.
Boar semen storage in vitro is the important step of Artificial Insemination Technology of Swine, and the quality of thinner is the principal element affecting the pig semen holding time.One of object of sperm dilution is the head number expanding breeding sow.When natural mating, boar can only breed for 1 time 1 sow, and once takes boar semen, and through dilution process, can breed 20-30 head sow under normal circumstances, and the pregnancy rate of sow can not reduce.The dilution powder major part of current domestic employing is the product from external import, also has a little part to be introduce production and processing voluntarily of filling a prescription.Dilution powder market is more chaotic, and dragons and fishes jumbled together, does not have evaluation criteria and the mechanism of specialization, makes the producer when buying dilution powder relatively blindly.Therefore, exploitation has the dilution powder product of independent intellectual property right, makes domestic best dilution powder brand, imperative.
Summary of the invention
The object of the invention is for above-mentioned technology, a boar frozen semen dilution powder is provided.
For solving the problem, the technical solution adopted in the present invention is:
One boar frozen semen dilution powder, is characterized in that: described dilution powder by D-Glucose, trisodium citrate, EDETATE SODIUM salt, potassium dihydrogen phosphate, potassium cyanide, streptomycin sulphate, polyvinyl alcohol (PVP.Type11), three carboxymethylamino methyl alcohol (Tris), citric acid, partly coil propylhomoserin, OEP (amino-sodium laurilsulfate), SDS (lauryl sodium sulfate), PGF2a, vitamin E, GSH (glutathione), hyaluronic acid, N-acetyl group-D-aminoglucose form.
Preferably, a described boar frozen semen dilution powder, its weight item is as follows:
Add temperature during use at 37 DEG C, containing the distilled water of the 1000g within 20 impurity, mix use.
This invention exploits the new formula of diluting boar semen agent, the composition that the yolk, skim milk powder etc. that replace in original diluent formulation with lecithin, fructose, glucose etc. are animal derived, and some other additive of conbined usage is optimized in research, such as, buffer: potassium dihydrogen phosphate, sodium citrate, Tris etc.; Antibacterial agent: penicillin, streptomycin etc.; Washing agent: OEP (amino-sodium laurilsulfate), SDS (lauryl sodium sulfate) etc.; Antioxidant: vitamin E, GSH (glutathione) etc.; Steroids composition: PGF2a; Other compositions: hyaluronic acid, N-acetyl group-D-aminoglucose etc., improve fertilization rate and the litter size of pig frozen semen, can improve the safety of product simultaneously, reduce the danger causing seminal fluid to infect because of the animal derived materials in thinner.
Embodiment
Below in conjunction with specific embodiment, do elaboration detailed further to the present invention, but implementation method of the present invention is not limited to the scope represented by embodiment, this embodiment is only limitted to the present invention is described, and not for limiting the scope of the invention.
One boar frozen semen dilution powder, described dilution powder by D-Glucose, trisodium citrate, EDETATE SODIUM salt, potassium dihydrogen phosphate, potassium cyanide, streptomycin sulphate, polyvinyl alcohol (PVP.Type11), three carboxymethylamino methyl alcohol (Tris), citric acid, partly coil propylhomoserin, OEP (amino-sodium laurilsulfate), SDS (lauryl sodium sulfate), PGF2a, vitamin E, GSH (glutathione), hyaluronic acid, N-acetyl group-D-aminoglucose form.
Embodiment:
By D-Glucose 11.5g, trisodium citrate 11.65g, EDETATE SODIUM salt 2.35g, potassium dihydrogen phosphate 1.75g, potassium cyanide 0.75g, streptomycin sulphate 0.5g, polyvinyl alcohol (PVP.Type11) 1.0g, three carboxymethylamino methyl alcohol (Tris) 5.5g, citric acid 4.1g, half dish propylhomoserin 0.07g, OEP (amino-sodium laurilsulfate) 0.4g, SDS (lauryl sodium sulfate) 0.3g, PGF2a 0.5g, vitamin E 0.5g, GSH (glutathione) 0.5g, hyaluronic acid 0.3g, N-acetyl group-D-aminoglucose 0.5g adds 1000g temperature at 37 DEG C, containing the redistilled water within 20 impurity, mix and use.
The result of use of above-described embodiment is as shown in the table:
The present invention is a kind of dilution powder when being specifically applied to boar semen, through experimental data display, due to sugar in thinner of the present invention by monose and disaccharide with the use of, ensure that acrosomal integrity and sperm freeze rear motility rate.This formula in use conventional glycerine be used as cryoprotector combine with the use of; improve fertilization rate and the litter size of pig frozen semen; improve the safety of product simultaneously; reduce the danger of the seminal fluid infection caused because of the animal derived materials in thinner; fill up the blank in this field domestic, there is profound significance.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are realized or uses the present invention, to be apparent for those skilled in the art to the multiple amendment of embodiment, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to embodiment illustrated herein, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (2)
1. a boar frozen semen dilution powder, is characterized in that: described dilution powder by D-Glucose, trisodium citrate, EDETATE SODIUM salt, potassium dihydrogen phosphate, potassium cyanide, streptomycin sulphate, polyvinyl alcohol (PVP.Typell), three carboxymethylamino methyl alcohol (Tris), citric acid, partly coil propylhomoserin, OEP (amino-sodium laurilsulfate), SDS (lauryl sodium sulfate), PGF2a, vitamin E, GSH (glutathione), hyaluronic acid, N-acetyl group-D-aminoglucose form.
2. a boar frozen semen dilution powder according to claim 1, it is characterized in that, its weight item is as follows:
Add temperature during use at 37 DEG C, containing the redistilled water of the 1000g within 20 impurity, mix use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410529108.XA CN104322485A (en) | 2014-10-10 | 2014-10-10 | Boar frozen semen diluted powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410529108.XA CN104322485A (en) | 2014-10-10 | 2014-10-10 | Boar frozen semen diluted powder |
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| CN104322485A true CN104322485A (en) | 2015-02-04 |
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| CN201410529108.XA Pending CN104322485A (en) | 2014-10-10 | 2014-10-10 | Boar frozen semen diluted powder |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105850985A (en) * | 2016-05-31 | 2016-08-17 | 山东鑫基牧业有限公司 | Porcine frozen semen diluting powder |
| CN106070184A (en) * | 2016-05-31 | 2016-11-09 | 山东鑫基牧业有限公司 | The freezing of a kind of pig semen and defreezing method |
| WO2017139909A1 (en) * | 2016-02-16 | 2017-08-24 | 权国波 | Freezing diluent for livestock semen |
| CN110622957A (en) * | 2019-10-10 | 2019-12-31 | 北京田园奥瑞生物科技有限公司 | Stable and efficient pig frozen semen reagent |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101392236A (en) * | 2008-11-06 | 2009-03-25 | 上海交通大学 | Antifreezer for diluting boar semen |
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2014
- 2014-10-10 CN CN201410529108.XA patent/CN104322485A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101392236A (en) * | 2008-11-06 | 2009-03-25 | 上海交通大学 | Antifreezer for diluting boar semen |
Non-Patent Citations (3)
| Title |
|---|
| 李旭主编: "《中国基层畜牧兽医管理全书》", 30 June 2006, article "（四十一）猪氢氰酸中毒", pages: 761 * |
| 柏惠等: "氰化钾对人精子线粒体细胞色素氧化酶作用的研究", 《南京医科大学学报》, vol. 20, no. 6, 30 November 2000 (2000-11-30), pages 458 - 459 * |
| 胡跃飞主编: "《现代有机合成试剂-性质、制备和反应》", 31 July 2006, article "氰化钾", pages: 262 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017139909A1 (en) * | 2016-02-16 | 2017-08-24 | 权国波 | Freezing diluent for livestock semen |
| CN105850985A (en) * | 2016-05-31 | 2016-08-17 | 山东鑫基牧业有限公司 | Porcine frozen semen diluting powder |
| CN106070184A (en) * | 2016-05-31 | 2016-11-09 | 山东鑫基牧业有限公司 | The freezing of a kind of pig semen and defreezing method |
| CN110622957A (en) * | 2019-10-10 | 2019-12-31 | 北京田园奥瑞生物科技有限公司 | Stable and efficient pig frozen semen reagent |
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Application publication date: 20150204 |