CN104094926A - Chicken semen diluent and preparation and utilization methods thereof - Google Patents
Chicken semen diluent and preparation and utilization methods thereof Download PDFInfo
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- CN104094926A CN104094926A CN201410380561.9A CN201410380561A CN104094926A CN 104094926 A CN104094926 A CN 104094926A CN 201410380561 A CN201410380561 A CN 201410380561A CN 104094926 A CN104094926 A CN 104094926A
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Abstract
Chicken semen diluent comprises 7.0 to 9.0 g per L of fructose, 4.0 to 6.0 g per L of sodium glutamate, 11.0 to 14.0 g per L of dipotassium phosphate, 0.5 to 0.7 g per L of monopotassium phosphate, 3.0 to 5.0 g per L of TES (N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid), 4.0 to 0.8 g per L of potassium citrate, 3.0 to 6.0 g per L of sodium acetate trihydrate, 0.2 to 0.4 g per L of anhydrous magnesium chloride, 0.05 to 0.1 g per L of lycopene. A PH (Potential of Hydrogen) value of the diluent is from 7.3 to 7.5 and the osmotic pressure is from 260 to 380 mOsm. The invention also provides preparation and utilization methods for the chicken semen diluent. The preparation and utilization methods for the chicken semen diluent are characterized in that the lycopene is added to the diluent, adjusting modes of the PH value and an osmotic pressure are changed, the diluent is stored in a cryopreservation mode, the semen deposition can be performed immediately after the chicken semen is diluted or after the cold storage, and the diluent also can be used as fundamental liquid for semen cryoprotectant. The chicken semen diluent has the advantages of being simple in preparation method, long in expiration data, free of antibiotics and stable in fertility rate.
Description
Technical field
The present invention relates to poultry propagation field, more concrete, relate to a kind of solution for dilution of cock semen and preparation thereof and using method.
Technical background
At present, in poultry propagation field, to chicken carry out artificial insemination used be nearly all undiluted chickens' extract liquid, on the one hand, the chickens' extract liquid of not diluted, the holding time exceedes half an hour, will affect fertilization rate; On the other hand, use through dilution chickens' extract liquid, the cost of poultry propagation is drawn high, and if the former essence of chicken can be diluted 1～2 times, can significantly save kind of a cock feeding cost.
In addition, in activity in production, plant chicken artificial insemination, often occur firm when added the sperm volume of cock semen collection inadequate, the later stage is because the too high sperm volume that also causes of death rate is inadequate.
The technology of artificial insemination of dilution chickens' extract liquid is a complicated system engineering, be subject to chickens' extract liquid kind, age in days, feeding environment, feed, the composition of dilution, the dilution ratio of chickens' extract liquid, the interval between insemination, diluent preparation method and preservation, insemination dose, the impact (Cao Jinyuan etc. 2013) of the factors such as the semen deposition degree of depth and semen collection number of times, in addition, Van Wambeke just once reported the successful case that chickens' extract liquid does not make significant difference to fertilization rate after dilution after 5～10 DEG C of preservation 24h in 1967, but prior art still exists solution for dilution of cock semen storage life short, even now with the current, lack technical standard, the problems such as fertilization rate is unstable.
A kind of chicken sperm dilution of patent of invention and preserving with powder and its preparation method and application (CN 101543209 A) although proposed a kind ofly for diluting and preserve product and the method for chickens' extract liquid, the actual operability of described product and method is little; And the described dilution that a kind of solution for dilution of cock semen of patent of invention and its preparation method and application (CN 102812950 A) proposes is 4 DEG C of preservations, shelf-life is short and added antibiotic in described dilution, applies difficulty and causes antibiotic residue.
In frozen chicken seminal fluid field, also face at present after thawing the fertilization rate of seminal fluid extremely low, even can not get the predicament of fertilization rate, also make to utilize frozen semen conserving species resource, significantly save the technology of conservation expense, also cannot realize in poultry propagation field.
Lycopene is a kind of phytochemical being extensively present in the fruit such as tomato, tomato product and watermelon, grapefruit, is a kind of well antioxidant (Xu Yue etc. 2012).
ROSATO equals to have delivered for 2012 and can improve low temperature about lycopene and preserve the report of the integrality of the vigor of turkey sperm, survival index, osmotic pressure resistance, DNA.But at present, also there is not the research report of lycopene to chicken semen freezing effect.
Summary of the invention
Still the problem and shortage existing in order to solve above-mentioned prior art, patent of the present invention has proposed a kind of solution for dilution of cock semen.
A kind of solution for dilution of cock semen, its chief component composition is as follows,
Add quartzy dual distilled water to 1.0L
Dilution described in patent of the present invention is made to further restriction, as follows,
A kind of solution for dilution of cock semen, also comprises composition lycopene 0.05～0.1g/L.
The pH value of described dilution is 7.3～7.5, and osmotic pressure is 260～380mOsm/kg.
Meanwhile, patent of the present invention has also proposed a kind of chicken sperm dilution liquid making method, and the method comprises
4.1), under aseptic condition, get fructose, sodium glutamate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, TES (N-trihydroxy methyl methyl-2-amino ethyl sulfonic acid), potassium citrate, Sodium acetate trihydrate, anhydrous magnesium chloride, lycopene;
4.2) osmotic pressure value adjustment;
4.3) pH value is adjusted;
4.4) under aseptic condition, by the described dilution packing preparing and be placed on that refrigerating chamber is freezing to be saved backup.
Using method described in patent of the present invention is made to further restriction, as follows,
The every increase by 10% of described constant volume, the osmotic pressure value of described dilution reduces 25mOsm.
The every increase of described potassium dihydrogen phosphate 0.7g/L, the pH value of described dilution declines 0.1.
In addition, patent of the present invention has also proposed a kind of solution for dilution of cock semen using method, described method,
5.1) described dilution is thawed in advance;
5.2) before dilution, described dilution is put into thermostat water bath the pre-heat treatment;
5.3) use described dilution chickens' extract liquid by dilution in 1: 1～1: 2.
Using method described in patent of the present invention is made to further restriction, as follows,
Chickens' extract liquid semen deposition immediately after described dilution.
Chickens' extract liquid after described dilution can be 5～10 DEG C of refrigeration semen depositions again after 6 hours.
Described dilution can be used as chicken frozen semen, the basal liquid of cryoprotector.
The inventive point of patent of the present invention is, the one, in described dilution, add antioxidant lycopene, and be conducive to dilution semen deposition after stored refrigerated after semen deposition immediately, sperm dilution after chicken sperm dilution, or as the protectant basal liquid of chicken semen freezing; The 2nd, in described compound method, change osmotic pressure and adjust mode, by adjusting volume when constant volume, instead of the content of adjusting single component adjusts osmotic pressure, is applicable to the chickens' extract liquid of different osmotic; The 3rd, in described compound method, change change pH values and adjust mode, by changing the ratio of potassium dihydrogen phosphate, instead of adjust pH value by HCl (hydrochloric acid solution) and/or NaOH (sodium hydroxide solution), be adapted to the chickens' extract liquid of different pH values; The 4th, described dilution is taked freezing preserving type, instead of adds after antibiotic the cold storage mode of 4 DEG C.
The beneficial effect of patent of the present invention is, the one, and compound method is simple; The 2nd, the long shelf-life of dilution, the shelf-life of described dilution is no less than 2 months; The 3rd, described dilution is not containing antibiotic; The 4th, fertilization rate is stable.
Specific embodiment
Specific embodiment 1
A kind of solution for dilution of cock semen, its constituent is as follows,
Add quartzy dual distilled water to 1.0L
Specific embodiment 2
From 50 cocks, select 20 as semen collection cock according to seminal fluid density and vigor quality.
Content with reference to [0024]～[0025] is prepared described a kind of solution for dilution of cock semen, obtains the chickens' extract liquid after diluting by 1: 1 and 1: 2 with reference to the content of [0014]～[0021] simultaneously.
Choose four groups of Meat-type Breeding Hens, respectively with pressing seminal fluid: dilution, chickens' extract liquid and the undiluted former smart semen deposition of 1: 1,1: 2,1: 1 (the freezing preservation of dilution 2 months) dilution, every group 200, every semen deposition in 5 days once, every hen semen deposition volume, the seminal fluid after dilution is identical with former essence, semen deposition three times, starts to receive egg for the 7th day from semen deposition for the first time continuously.Industrial experimentation the results are shown in following table 1.
The fertility rate of hatching egg statistics of table 1 different diluent
Claims (10)
1. a solution for dilution of cock semen, it is characterized in that described dilution main component is as follows,
Add quartzy dual distilled water to 1.0L.
2. dilution according to claim 1, characterized by further comprising lycopene 0.05～0.1g/L.
3. dilution according to claim 1 and 2, the pH value that it is characterized in that described dilution is 7.3～7.5, osmotic pressure is 260～380mOsm.
4. a chicken sperm dilution liquid making method, is characterized in that described method,
4.1), under aseptic condition, get fructose, sodium glutamate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, TES (N-trihydroxy methyl methyl-2-amino ethyl sulfonic acid), potassium citrate, Sodium acetate trihydrate, anhydrous magnesium chloride, lycopene;
4.2) osmotic pressure value adjustment;
4.3) pH value is adjusted;
4.4) under aseptic condition, by the described dilution packing preparing and be placed on that refrigerating chamber is freezing to be saved backup.
5. compound method according to claim 4, is characterized in that osmotic pressure value completes by adjusting constant volume.
6. compound method according to claim 4, is characterized in that pH value is to complete by the ratio of change potassium dihydrogen phosphate.
7. a using method for solution for dilution of cock semen, is characterized in that described method,
5.1) described dilution is thawed in advance;
5.2) before dilution, described dilution is put into the processing of thermostat water bath constant temperature;
5.3) use described dilution chickens' extract liquid by dilution in 1: 1～1: 2.
8. using method according to claim 5, is characterized in that, the chickens' extract liquid semen deposition immediately after described dilution.
9. using method according to claim 5, is characterized in that, the chickens' extract liquid after described dilution can be 5～10 DEG C of refrigeration semen depositions again after 6 hours.
10. using method according to claim 5, is characterized in that, described semen diluent can be used as the basal liquid of bird semen cryoprotectant.
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| CN201410380561.9A CN104094926A (en) | 2014-08-05 | 2014-08-05 | Chicken semen diluent and preparation and utilization methods thereof |
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| CN201410380561.9A CN104094926A (en) | 2014-08-05 | 2014-08-05 | Chicken semen diluent and preparation and utilization methods thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105076112A (en) * | 2015-08-12 | 2015-11-25 | 江西华裕家禽育种有限公司 | Chicken' semen diluent and application thereof |
| CN106538510A (en) * | 2016-09-22 | 2017-03-29 | 西北农林科技大学 | The application of lycopene and sesamol compatibility in domestic animal semen cryopreservation agent |
| CN107251892A (en) * | 2017-05-25 | 2017-10-17 | 中国热带农业科学院热带作物品种资源研究所 | It is a kind of suitable for the solution for dilution of cock semen of torrid areas, preparation and its application process |
| CN108566945A (en) * | 2017-06-26 | 2018-09-25 | 常州市四季禽业有限公司 | Yellow-feather broiler semen diluent and its preparation and application |
| CN111406738A (en) * | 2020-05-09 | 2020-07-14 | 华南农业大学 | Chicken essence diluent for artificial oviduct insemination and preparation method thereof |
| CN112314592A (en) * | 2020-10-28 | 2021-02-05 | 贵州凤集生态农业科技有限公司 | Egg chicken semen diluent and diluting method |
| CN112852720A (en) * | 2021-02-04 | 2021-05-28 | 西北农林科技大学 | Sheep X, Y sperm sorting solution, sorting planktonic system and sorting method |
| CN114794082A (en) * | 2022-04-16 | 2022-07-29 | 贵州省种畜禽种质测定中心 | Antifreeze protein-containing diluent for improving preservation quality of chicken essence and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080199846A1 (en) * | 2007-02-09 | 2008-08-21 | Julie Long | Poultry semen extender |
| CN101543209A (en) * | 2009-03-31 | 2009-09-30 | 华南农业大学 | Powder for chicken sperm dilution and preservation and preparation method and application thereof |
| CN102812950A (en) * | 2012-09-07 | 2012-12-12 | 江苏省家禽科学研究所 | Chicken semen diluent as well as preparation method and application thereof |
-
2014
- 2014-08-05 CN CN201410380561.9A patent/CN104094926A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080199846A1 (en) * | 2007-02-09 | 2008-08-21 | Julie Long | Poultry semen extender |
| CN101543209A (en) * | 2009-03-31 | 2009-09-30 | 华南农业大学 | Powder for chicken sperm dilution and preservation and preparation method and application thereof |
| CN102812950A (en) * | 2012-09-07 | 2012-12-12 | 江苏省家禽科学研究所 | Chicken semen diluent as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| MARIA GRAZIA MANGIAGALLI等: ""Effect of lycopene on fowl sperm characteristics during in vitro storage"", 《EUROPEAN POULTRY SCIENCE》, 31 December 2007 (2007-12-31), pages 27 - 2 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105076112A (en) * | 2015-08-12 | 2015-11-25 | 江西华裕家禽育种有限公司 | Chicken' semen diluent and application thereof |
| CN106538510A (en) * | 2016-09-22 | 2017-03-29 | 西北农林科技大学 | The application of lycopene and sesamol compatibility in domestic animal semen cryopreservation agent |
| CN107251892A (en) * | 2017-05-25 | 2017-10-17 | 中国热带农业科学院热带作物品种资源研究所 | It is a kind of suitable for the solution for dilution of cock semen of torrid areas, preparation and its application process |
| CN108566945A (en) * | 2017-06-26 | 2018-09-25 | 常州市四季禽业有限公司 | Yellow-feather broiler semen diluent and its preparation and application |
| CN111406738A (en) * | 2020-05-09 | 2020-07-14 | 华南农业大学 | Chicken essence diluent for artificial oviduct insemination and preparation method thereof |
| CN112314592A (en) * | 2020-10-28 | 2021-02-05 | 贵州凤集生态农业科技有限公司 | Egg chicken semen diluent and diluting method |
| CN112852720A (en) * | 2021-02-04 | 2021-05-28 | 西北农林科技大学 | Sheep X, Y sperm sorting solution, sorting planktonic system and sorting method |
| CN114794082A (en) * | 2022-04-16 | 2022-07-29 | 贵州省种畜禽种质测定中心 | Antifreeze protein-containing diluent for improving preservation quality of chicken essence and preparation method thereof |
| CN114794082B (en) * | 2022-04-16 | 2022-10-18 | 贵州省种畜禽种质测定中心 | Antifreeze protein-containing diluent for improving preservation quality of chicken essence and preparation method thereof |
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Application publication date: 20141015 |