CN103843759B - Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent - Google Patents
Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent Download PDFInfo
- Publication number
- CN103843759B CN103843759B CN201410083498.2A CN201410083498A CN103843759B CN 103843759 B CN103843759 B CN 103843759B CN 201410083498 A CN201410083498 A CN 201410083498A CN 103843759 B CN103843759 B CN 103843759B
- Authority
- CN
- China
- Prior art keywords
- solution
- chicken
- semen
- grams
- high altitude
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to chicken seminal fluid diluent applicable to high altitude localities. The chicken seminal fluid diluent is composed of a solution A and a solution B in a volume ratio of 50: (5-6), wherein the solution A is prepared from the following components: 8-9 grams of sodium acetate trihydrate, 18-22 grams of sodium glutamate monohydrate, 9-10 grams of sodium chloride, 10-15 grams of glucose and 10-15 grams of sodium citrate based on each liter of purified water; the solution B is prepared from the following components: 2 grams of sodium percarbonate and 2ml of glycerinum with the volume percentage of 5% based on each liter of purified water. The invention also provides a particular application method of the chicken seminal fluid diluent applicable to high altitude localities. Compared with an existing chicken seminal fluid diluent, the chicken seminal fluid diluent disclosed by the invention has the advantages of being better applicable to anoxic and cold environments of the high altitude localities, and being suitable for immediate insemination of the diluted seminal fluid and also suitable for insemination of diluted seminal fluid after short-period storage and short-distance transportation.
Description
Technical field
The present invention relates to poultry farming, particularly relate to a kind of Chicken Semen being suitable for high altitude localities and release thin liquid and preparation method thereof and application.
Background technology
Hide chicken, be distributed widely in the original chicken breed in place that the artificial selection degree of high altitude localities (1500 meters ~ 3000 meters) is very low, have the raising history of more than 1000 year in China.Hide the long and short legs of chicken build, head height tail is low, and chest muscle is flourishing, and property is active, more neurotic, bellicose, kindly ascends a height and circles in the air.Hide chicken matter extremely delicious, cultivation scale is less, is very applicable to high-end consumption market.Having a most important factor to result in Tibetan chicken to be at present difficult to enter high-end consumption market, is hide chicken sum less.
Cultural technique, relied on heredity to select to improve the egg production of chicken, rely on artificial fertilization technology to substantially increase the fertilization rate of chicken, thus improve incubation rate, expand the colony of chicken, thus the quantity improving chicken supplied market the present age.
The lack of quantity of spermatic fluid of cock, sperm concentration is large, and the seminal fluid at every turn adopted can only give the semen deposition of minority hen, and can expand with solution for dilution of cock semen dilution and measure to hen semen deposition accordingly, improve the availability of excellent cock, the breeding for chicken group has leading role.Solution for dilution of cock semen can also supplement the nutrients material, keeps the change of osmotic pressure that seminal fluid is suitable and buffering seminal fluid pH.Cock spermatozoa can keep fertility for a long time in hen body, and being easy to lose fertility in vitro, its reason is when semen collection, and the various compositions of cloaca body of gland are mixed among seminal fluid, cause that sperm motility is accelerated, energy reduces fast, and cause fertility to reduce.Suitable dilution can the nutrient source of energy as a supplement.Sperm produces and have accumulated carbonic acid gas and lactic acid in metabolic process, and the pH of seminal fluid is reduced, and some enzyme of sperm is suppressed, causes the fertility of sperm to reduce, even dead.Use in energy and the dilution of lactic acid, the effect of buffering can be reached.Application dilution means, the osmotic pressure that seminal fluid can also be kept suitable and electrolytical balance.
So why, hide the still rare numbers of chicken? one is that egg production is low because hide chicken major part without seed selection, and two is that the fertilization rate of the artificial insemination of high altitude localities is lower.And one of lower basic reason of the fertilization rate of the artificial insemination of high altitude localities lacks a kind of solution for dilution of cock semen being applicable to high altitude localities in actual production.
Summary of the invention
The object of this invention is to provide a kind of Chicken Semen being suitable for high altitude localities and release thin liquid and preparation method thereof and application.
The invention provides a kind of Chicken Semen being suitable for high altitude localities and release thin liquid, is 50:(5-6 by volume ratio) solution A and solution B form;
Described solution A is made up of following composition: add in often liter of pure water Sodium acetate trihydrate 8-9 gram, sodium glutamate monohydrate 18-22 gram, sodium chloride 9-10 gram, glucose 10-15 gram, sodium citrate 10-15 gram;
Described solution B is made up of following composition: the glycerine 2ml adding SODIUM PERCARBONATE 2 grams, volumn concentration 5% in often liter of pure water.
Described solution A is preferably as follows composition and makes: add Sodium acetate trihydrate 9 grams, sodium glutamate monohydrate 20 grams, 10 grams, sodium chloride, glucose 15 grams, sodium citrate 12 grams in often liter of pure water.
The Chicken Semen being suitable for high altitude localities described in the present invention also provides releases the application of thin liquid in the artificial insemination of high altitude localities chicken.Described chicken preferably hides chicken.
The concrete steps of described application are as follows:
1) obtain solution A in advance; When gathering Chicken Semen, 30 minutes ahead of time configuration solution B; The Chicken Semen that mixed solution A and solution B obtain being suitable for high altitude localities releases thin liquid, and be heated to 40 DEG C of insulations, adjust ph is 6.8-7.2, for subsequent use;
2) gather Chicken Semen, then according to volume ratio 1:(2-8) ratio with the Chicken Semen being suitable for high altitude localities that step 1) is standby release thin liquid dilution, obtain dilute after Chicken Semen;
3) step 2) gained dilution after Chicken Semen semen deposition immediately or short-term preservation after semen deposition; During semen deposition, get the Chicken Semen 0.1ml after dilution and instill in hen cloaca.
Wherein, obtain solution A described in step 1), for adding Sodium acetate trihydrate, sodium glutamate monohydrate, sodium chloride, glucose, sodium citrate in pure water, low-speed centrifugal 5 minutes, obtains solution A.
The speed of described low-speed centrifugal is 1500 ~ 3000 revs/min.
Wherein, obtain solution B described in step 1), for adding the glycerine of SODIUM PERCARBONATE, volumn concentration 5% in pure water, mixing obtains solution B.
Wherein, mixed solution A described in step 1) and solution B, for according to solution A: the volume ratio of solution B is 50:(5-6) ratio, while rock solution A limit to add solution B, the Chicken Semen that mixing obtains being suitable for high altitude localities releases thin liquid.
Wherein, adjust ph described in step 1), is preferably adjusted to 7.0.
Wherein, short-term preservation described in step 3), the holding time is 24 hours at most.
Wherein, short-term preservation described in step 3), the temperature of preservation is 0-5 DEG C.
Wherein, short-term preservation described in step 3), is guaranteeing, in temperature and holding time satisfactory situation, can do and transport.
The present invention has following beneficial effect:
Solution for dilution of cock semen of the present invention is compared with existing solution for dilution of cock semen, can better adapt to the environment of high altitude localities anoxic, severe cold (low temperature).This be due to: have employed SODIUM PERCARBONATE and glycerine, the former can produce oxygen in aqueous, the time that the oxygen that the latter can extend SODIUM PERCARBONATE generation under the hypobaric condition in high altitude localities retains in the solution, improve the dissolved oxygen content of solution B, and when Chicken Semen will be gathered, within 30 minutes ahead of time, configuration solution B then avoids the problem due to resting period long dissolved oxygen effusion, and the effect reached is that in the solution for dilution of cock semen of joining, dissolved oxygen content is high.Carry out the artificial insemination of chicken with solution for dilution of cock semen of the present invention in high altitude localities, than use existing solution for dilution of cock semen lifting dissolved oxygen content, substantially increase fertility rate and hatchability.
Make the Chicken Semen of 1:2 dilution with semen diluent of the present invention, in high altitude localities, semen deposition immediately and preservation semen deposition after 24 hours at 4 DEG C, the incubation rate hiding chicken is respectively 79% and 62%, significantly higher than the fertility rate of egg (48%) of former essence semen deposition immediately.The present invention is suitable for semen deposition immediately after dilution, does semen deposition after short-term preservation (in 24 hours) and short-distance transport after being also suitable for dilution.Semen diluent composition of the present invention is few, and preparation is simple, draws materials easily, cheap, is easy to promote.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.Embodiment Sodium acetate trihydrate used, sodium glutamate monohydrate, sodium chloride, glucose, sodium citrate, SODIUM PERCARBONATE 2 grams, 5% glycerine all open Reagent Company purchased from Yaan Rui Jinte company and Yaan.
Embodiment 1 is prepared the Chicken Semen that the present invention is suitable for high altitude localities and is released thin liquid 1
Solution A is made up of following composition: add Sodium acetate trihydrate 9 grams, sodium glutamate monohydrate 20 grams, 10 grams, sodium chloride, glucose 15 grams, sodium citrate 12 grams in often liter of pure water;
Solution B is made up of following composition: the glycerine 2ml adding SODIUM PERCARBONATE 2 grams, volumn concentration 5% in often liter of pure water.
According to solution A: the volume ratio of solution B is 10:1(and 50:5) ratio, while rock solution A limit to add solution B, the Chicken Semen that mixing obtains being suitable for high altitude localities releases thin liquid 1.
Embodiment 2 is prepared the Chicken Semen that the present invention is suitable for high altitude localities and is released thin liquid 2
Solution A is made up of following composition: add Sodium acetate trihydrate 8 grams, sodium glutamate monohydrate 22 grams, 10 grams, sodium chloride, glucose 15 grams, sodium citrate 15 grams in often liter of pure water;
Solution B is made up of following composition: the glycerine 2ml adding SODIUM PERCARBONATE 2 grams, volumn concentration 5% in often liter of pure water.
According to solution A: the volume ratio of solution B is the ratio of 50:6, while rock solution A limit to add solution B, the Chicken Semen that mixing obtains being suitable for high altitude localities releases thin liquid 2.
The Chicken Semen that embodiment 3 the present invention is suitable for high altitude localities releases the extension rate of thin liquid
The Chicken Semen that described the present invention is suitable for high altitude localities releases the formula of thin liquid according to embodiment 1, obtain solution A in advance; When gathering Chicken Semen, 30 minutes ahead of time configuration solution B; The Chicken Semen that mixed solution A and solution B obtain being suitable for high altitude localities releases thin liquid, is heated to 40 DEG C of insulations, and adjustment PH is that 7.0(adjustment is carried out with the NAOH solution of the HCl solution of mass percent 1-2% or mass percent 1-2%), for subsequent use.
Arrange 8 extension rates, be specially 1:1(and namely get the seminal fluid that volume is V, adding volume is that the Chicken Semen of the present invention of V is released thin liquid and diluted, lower with), 1:2,1:3,1:4,1:5,1:6,1:7,1:8.
Test site is in Xiangcheng County, be positioned at the west and south, Tibetan Autonomous Prefecture of Garze, Sichuan Province, height above sea level 2865 meters, temperature 16 DEG C during test, adopt hen in the raising layer chicken phase of 240 days, each ratio 3 hens, after adopting the seminal fluid of same cock, dilute respectively according to extension rate, giving hen semen deposition at twice, is once semen deposition immediately after processing, another time preserves semen deposition after 24 hours under 4 DEG C of environment, often group adopts the insulation that uses the same method, and semen deposition every hen semen deposition 0.1ml(drips 1 with glue head dropper), every hen collects about 6 ~ 9 of egg.The kind egg same time collected by twice semen deposition detects fertilization rate by the method for " according to egg ", the results are shown in Table 1.
The fertilization rate of the different dilution ratio of table 1
| Chicken Semen and dilution ratio | 1:1 | 1:2 | 1：3 | 1：4 | 1：5 | 1：6 | 1：7 | 1：8 |
| Fertilization rate % immediately after semen deposition | 70 | 79 | 77 | 74 | 71 | 70 | 68 | 68 |
| Fertilization rate % after semen deposition in 24 hours | 52 | 62 | 53 | 37 | 33 | 28 | 22 | 19 |
Result shows, dilution 1:(1-8) all can obtain good effect, when especially dilution ratio is 1:2, the fertilization rate immediately after semen deposition is the fertilization rate after semen deposition in 79%, 24 hours is 62%.
The Chicken Semen that embodiment 4 the present invention is suitable for high altitude localities releases the application of thin liquid
Test site is in Xiangcheng County, be positioned at the west and south, Tibetan Autonomous Prefecture of Garze, Sichuan Province, height above sea level 2865 meters, temperature 16 DEG C during test, adopts hen 200 in the raising layer chicken phase of 240 days, cock 16, be divided into 4 groups, often organize cock 4, hen 50, whole process of the test is by same semen collection person's semen collection, and cock extraction sperm volume is 0.3ml to 2ml.After extraction seminal fluid according to every group to the processing method of seminal fluid, at twice to 25 of this group hen semen depositions, it is once semen deposition immediately after processing, another time preserves semen deposition after 24 hours under 4 DEG C of environment, often group adopts the insulation that uses the same method, semen deposition every hen semen deposition 0.1ml(drips 1 with glue head dropper), in method of operating, only difference is that the dilution of seminal fluid is different.The kind egg same time collected by twice semen deposition detects fertilization rate by the method for " according to egg ".
The dilution process of different seminal fluid is as follows:
(1) not add semen diluent for contrast.
(2) physiological saline (namely get the seminal fluid that volume is V, adding volume is that the physiological saline of 2V dilutes, lower with) is added according to 1:2.Physiological saline is provided by poultry scientific research breeding factory of Sichuan Agricultural University.
(3) add common semen diluent according to 1:2, common semen diluent is provided by poultry scientific research breeding factory of Sichuan Agricultural University, and formula is add 9 grams, sodium chloride, glucose 22 grams, sodium citrate 15 grams in 1 premium on currency.
(4) add according to 1:2 the Chicken Semen that the present invention is suitable for high altitude localities and release thin liquid, the Chicken Semen that described the present invention is suitable for high altitude localities releases the formula of thin liquid according to embodiment 1, obtain solution A in advance; When gathering Chicken Semen, 30 minutes ahead of time configuration solution B; The Chicken Semen that mixed solution A and solution B obtain being suitable for high altitude localities releases thin liquid, is heated to 40 DEG C of insulations, and adjustment PH is 7.0, for subsequent use.
Often group liquefacient duration method and incubation rate the results are shown in Table 2:
Fertilization rate after the semen deposition of table 2 different diluent
This test is in process of the test, and process (1), because seminal fluid is without any process, collecting semen amount is less, and underweight is to complete test, so it is lower to cause fertilization rate.The former seminal fluid of chicken, as without any process, in outside air, just loses activity after usual 6 hours.So fertilization rate is 0 after 24 hours.
Adopt the fertilization rate (62%) of the fertilization rate (79%) after dilution of the present invention semen deposition immediately and semen deposition after 24 hours all higher than other process.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. the Chicken Semen being suitable for high altitude localities releases a thin liquid, it is characterized in that, it is 50:(5-6 that this Chicken Semen releases thin liquid by volume ratio) solution A and solution B form;
Described solution A is made up of following composition: add in often liter of pure water Sodium acetate trihydrate 8-9 gram, sodium glutamate monohydrate 18-22 gram, sodium chloride 9-10 gram, glucose 10-15 gram, sodium citrate 10-15 gram;
Described solution B is made up of following composition: the glycerine 2ml adding SODIUM PERCARBONATE 2 grams, volumn concentration 5% in often liter of pure water.
2. the Chicken Semen being suitable for high altitude localities according to claim 1 releases thin liquid, it is characterized in that, described solution A is made up of following composition: add Sodium acetate trihydrate 9 grams, sodium glutamate monohydrate 20 grams, 10 grams, sodium chloride, glucose 15 grams, sodium citrate 12 grams in often liter of pure water.
3. the Chicken Semen being suitable for high altitude localities as described in any one of claim 1 or 2 releases the application of thin liquid in the artificial insemination of high altitude localities chicken; Concrete steps are as follows:
1) obtain solution A in advance; When gathering Chicken Semen, 30 minutes ahead of time configuration solution B; The Chicken Semen that mixed solution A and solution B obtain being suitable for high altitude localities releases thin liquid, and be heated to 40 DEG C of insulations, adjust ph is 6.8-7.2, for subsequent use;
2) gather Chicken Semen, then according to volume ratio 1:(2-8) ratio with step 1) the standby Chicken Semen being suitable for high altitude localities release thin liquid dilution, obtain dilute after Chicken Semen;
3) step 2) gained dilution after Chicken Semen semen deposition immediately or short-term preservation after semen deposition; During semen deposition, get the Chicken Semen 0.1ml after dilution and instill in hen cloaca.
4. application according to claim 3, is characterized in that, step 1) described obtain solution A, for adding Sodium acetate trihydrate, sodium glutamate monohydrate, sodium chloride, glucose, sodium citrate in pure water, low-speed centrifugal 5 minutes, obtains solution A.
5. application according to claim 4, is characterized in that, the speed of described low-speed centrifugal is 1500 ~ 3000 revs/min.
6. apply described in claim 3, it is characterized in that, step 1) described obtain solution B, for adding the glycerine of SODIUM PERCARBONATE, volumn concentration 5% in pure water, mixing obtains solution B.
7. apply described in claim 3, it is characterized in that, step 1) described mixed solution A and solution B, for according to solution A: the volume ratio of solution B is 50:(5-6) ratio, while rock solution A limit to add solution B, the Chicken Semen that mixing obtains being suitable for high altitude localities releases thin liquid.
8. apply described in claim 3, it is characterized in that, step 1) described adjust ph, for being adjusted to 7.0.
9. apply described in claim 3, it is characterized in that, step 3) described short-term preservation, the holding time is 24 hours at most, and the temperature of preservation is 0-5 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410083498.2A CN103843759B (en) | 2014-03-07 | 2014-03-07 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410083498.2A CN103843759B (en) | 2014-03-07 | 2014-03-07 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103843759A CN103843759A (en) | 2014-06-11 |
| CN103843759B true CN103843759B (en) | 2015-05-20 |
Family
ID=50852217
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410083498.2A Expired - Fee Related CN103843759B (en) | 2014-03-07 | 2014-03-07 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103843759B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105287045B (en) * | 2015-10-15 | 2017-01-04 | 燕海峰 | A kind of semen dilution method that can be used for kind of chicken house artificial insemination |
| CN106420104B (en) * | 2016-09-20 | 2017-12-19 | 四川农业大学 | Semen collection cup is hit in one breeder electricity consumption |
| CN106175977B (en) * | 2016-09-20 | 2017-12-19 | 上海丰蓓生物科技有限公司 | A kind of breeder sperm extractor tool |
| CN107913118A (en) * | 2017-12-19 | 2018-04-17 | 佛山科学技术学院 | A kind of mule duck artificial insemination method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86101836A (en) * | 1986-03-22 | 1987-01-24 | 中国农业科学院畜牧研究所 | Solution for dilution of cock semen |
| JP3636609B2 (en) * | 1999-02-24 | 2005-04-06 | 日本全薬工業株式会社 | Pig semen preservation solution and method of use thereof |
| CN1814749A (en) * | 2005-02-04 | 2006-08-09 | 谢来安 | Instant pork-essence liquor diluting solution |
| CN101543209A (en) * | 2009-03-31 | 2009-09-30 | 华南农业大学 | Powder for chicken sperm dilution and preservation and preparation method and application thereof |
| CN102696577A (en) * | 2011-08-18 | 2012-10-03 | 北京富通华投资有限公司 | Bovine semen protective agent |
| CN102812950A (en) * | 2012-09-07 | 2012-12-12 | 江苏省家禽科学研究所 | Chicken semen diluent as well as preparation method and application thereof |
-
2014
- 2014-03-07 CN CN201410083498.2A patent/CN103843759B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86101836A (en) * | 1986-03-22 | 1987-01-24 | 中国农业科学院畜牧研究所 | Solution for dilution of cock semen |
| JP3636609B2 (en) * | 1999-02-24 | 2005-04-06 | 日本全薬工業株式会社 | Pig semen preservation solution and method of use thereof |
| CN1814749A (en) * | 2005-02-04 | 2006-08-09 | 谢来安 | Instant pork-essence liquor diluting solution |
| CN101543209A (en) * | 2009-03-31 | 2009-09-30 | 华南农业大学 | Powder for chicken sperm dilution and preservation and preparation method and application thereof |
| CN102696577A (en) * | 2011-08-18 | 2012-10-03 | 北京富通华投资有限公司 | Bovine semen protective agent |
| CN102812950A (en) * | 2012-09-07 | 2012-12-12 | 江苏省家禽科学研究所 | Chicken semen diluent as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 不同稀释液2—5℃保存鸡精液的研究;朱庆等;《四川农业大学学报》;19851031;第3卷(第2期);第463-467页 * |
| 不同稀释液及稀释倍数对鸡蛋受精率的影响;张珊等;《中国家禽科学研究进展——第十四次全国家禽科学学术讨论会论文集》;20091231;第125-133页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103843759A (en) | 2014-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103314904B (en) | Large gauge Eriocheir sinensia pond ecological seedling-cultivating method | |
| CN103843759B (en) | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent | |
| CN103202127B (en) | Method for stopping Taxus chinensis var. mairei seed dormancy | |
| CN103202214B (en) | Breeding method of sargassum vachellianum artificial seeds | |
| CN104686860B (en) | A kind of nutriment extending aphidius gifuensis life span of adult | |
| CN104737971B (en) | Method for culturing litopenaeus vannamei through water body osmotic pressure regulator | |
| CN102939943A (en) | Artificial propagation method for myzus persicae | |
| CN104094926A (en) | Chicken semen diluent and preparation and utilization methods thereof | |
| CN103891711B (en) | The processing method of flower perch seminal fluid | |
| CN101543211A (en) | Cut flower preservative and application thereof | |
| CN102293338A (en) | Artificial forage for larvae of Holotrichia parallcla, preparation method thereof, and application thereof | |
| CN106719170B (en) | Sturgeon hatching method | |
| CN103960182A (en) | Method for establishing exopalaemon carinicauda family by applying in-vitro egg culture | |
| CN109722407B (en) | Method for compensating growth of microalgae by starting at ice temperature | |
| CN103518775B (en) | A kind of Seabuckthorn growth regulator | |
| CN102246747A (en) | Semen diluter and preparation method as well as application method thereof | |
| CN105432565A (en) | Hatching method for increasing energy reserve of commercial generation hatching eggs of white feather broilers | |
| CN102326673B (en) | Method for producing aerobic fermentation photosynthetic bacteria livestock culturing feed additive | |
| CN105052792A (en) | High-yield breeding method for clams | |
| CN106035090B (en) | A kind of Chunlan root-like stock quick breeding method for tissue culture | |
| CN104585102A (en) | High-yield culture method of whitebait | |
| CN103283654A (en) | Method for increasing settlement and metamorphosis rate in Apostichopus japonicus larvae | |
| CN107897061A (en) | A kind of cultural method of Epinephelus coioides | |
| CN105036908A (en) | Pleurotus nebrodensis mother seed medium | |
| CN108203696A (en) | A kind of orange ocean rhodotorula process for preparation of dry powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150520 Termination date: 20160307 |