CN86101836A - Solution for dilution of cock semen - Google Patents
Solution for dilution of cock semen Download PDFInfo
- Publication number
- CN86101836A CN86101836A CN86101836.2A CN86101836A CN86101836A CN 86101836 A CN86101836 A CN 86101836A CN 86101836 A CN86101836 A CN 86101836A CN 86101836 A CN86101836 A CN 86101836A
- Authority
- CN
- China
- Prior art keywords
- liquid
- dilution
- egg
- semen
- semen deposition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Abstract
The invention belongs to solution for dilution of cock semen, include glucose, sodium citrate, potassium dihydrogen phosphate, sodium hydrogen phosphate and distilled water.The chicken essence liquid that the present invention did 1: 2 and diluted at 1: 1, semen deposition after preserving 7 and 24 hours respectively under 5 ℃, fertility rate of egg is respectively 94.7% and 92.2%, with the no marked difference of fertility rate of egg (95.5%) of former essence semen deposition immediately.Dilute immediately semen deposition at 1: 2 if reduce consumption and do, fertility rate of egg is 95.9%, has reached the fertility of former essence.The present invention is suitable for semen deposition immediately after the dilution, also be suitable for doing after the dilution short-term is preserved and short-distance transport after semen deposition.Solution composition is few, and preparation is simple, draws materials easily, and is cheap, is easy to promote.
Description
The invention belongs to the poultry semen diluent, particularly belong to solution for dilution of cock semen.
Along with the expansion of the scale of raising chickens and the utilization of captive management technology, the technology of artificial insemination of chicken is extensively carried out in poultry husbandry.For improving utilization rate and the test-tube work efficiency of kind of cock, many countries are in the chicken essence liquid semen deposition of adopting through diluted.Ideal diluent is not make chicken essence forfeiture insemination ability, draws materials easily, and preparation is simple, and cost is low, is convenient to promote.Be mixed with such diluent, be pursuing one's goal of poultry breeding man in decades always.Russian Patent SU638334 diluent (1) (hereinafter to be referred as 638334 liquid) is arranged in this respect.This liquid is formulated by anhydrous medical glucose, sodium acetate, sodium bicarbonate, 10% acetic acid and distilled water.But with chicken essence of this liquid dilution, 2 ℃～4 ℃ preservation semen depositions after 7 hours down, fertility rate of egg just reaches 91.8%, and the acid-base value instability of solution.According to CA(ChemicalAbstracts) chemical abstracts, the BPSE diluent (2) of the U.S. can make chicken essence survive 18 hours down at external 5 ℃～15 ℃, and does not lose fertility.The composition of this diluent is fructose, magnesium chloride, sodium acetate, sodium citrate, sodium glutamate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, TES(N-tris (Hydroxymethyl) methyl-2-aminoethanesulfonicacid) and distilled water.It is generally acknowledged that BPSE liquid is a kind of diluent preferably of dilution chicken essence liquid.But since wherein used sodium glutamate and TES not only price is high, also be difficult to buy, it is used widely is restricted.To this diluent, except that digest, do not see other relevant documents, thereby, be difficult to be further described.For this liquid being done further analysis, carried out contrast test at the present invention.Find that the survival index of its chicken essence is not high enough with the chicken essence liquid of this liquid dilution.
For overcoming above-mentioned shortcoming, carried out the present invention.The objective of the invention is to improve the survival index of chicken essence in diluent and keep its normal fertility, and diluent is drawn materials easily, price is cheap, helps promotion and application.For this reason, in containing potassium dihydrogen phosphate and distilled water and fowl semen diluent, replenish adding glucose, sodium citrate and sodium hydrogen phosphate.Dextrose components wherein be sperm at external metabolic energy source, phosphate is buffer system, makes the dilution seminal fluid keep certain acid-base value and buffering ability, sodium citrate is kept the Osmotic balance of diluent environment and the interior environment of sperm.The content of every composition is in the diluent:
Glucose 1.20-1.40 gram
Sodium citrate 1.20-1.40 gram
Potassium dihydrogen phosphate 0.15-0.40 gram
Sodium hydrogen phosphate 2.00-2.40 gram
Distilled water adds to 100 milliliters
pH 7.10-7.50
Osmotic pressure 300-370mos/kg
Suitable diluent is named as BJJX liquid, and its component content is:
Glucose 1.40 grams
Sodium citrate 1.40 grams
Potassium dihydrogen phosphate 0.36 gram
Sodium hydrogen phosphate 2.40 grams
Distilled water adds to 100 milliliters
The content of above-mentioned pH7.5 osmotic pressure 365mos/kg diluent can also reduce, and names to be BJJX-II liquid, and every component content is as follows:
Glucose 1.20 grams
Sodium citrate 1.20 grams
Potassium dihydrogen phosphate 0.16 gram
Sodium hydrogen phosphate 2.08 grams
Distilled water adds to 100 milliliters
pH 7.45
Osmotic pressure 310mos/kg
The present invention prepares simply, and content adds mentioned component in accordance with regulations, and adding distil water again is made into 100 ml solns.
Preserve test (as shown in table 1) according to chicken essence liquid, in 33 ℃ of waters bath with thermostatic control, the survival index average out to 4.71 of its chicken essence occupies first of each group of test with the chicken essence liquid of BJJX liquid dilution, survival index than the former essence of not diluted is high by 1.87, and the survival index of former essence is 2.84.Illustrate that BJJX liquid is suitable for the existence of chicken essence, can be used for preserving chicken essence liquid.The computational methods of survival index:
Be after dilution chicken essence liquid is placed above-mentioned water-bath, every one hour observation vigor once, maybe can find out between group till the difference to whole sperm death.During statistics, vigor and this vigor sum SCK two removed with last time, multiply by the summation of twice interval again, promptly got survival index.
According to fertility rate of egg contrast test (as table 2),, under 5 ℃, to preserve and gave the hen semen deposition in 7 hours by 1: 2 dilution chicken essence liquid with BJJX liquid, fertility rate of egg is 94.7%; Be diluted in 5 ℃ by 1: 1 and preserved 24 hours down, fertility rate of egg is 92.2%, and by dilution in 1: 2, fertility rate of egg was 90.6%; And with the semen deposition immediately of former essence, fertility rate of egg is 95.5%.Illustrate with the dilution of BJJX liquid and preserved at low temperatures 7 hours that the egg fertilization can reach bright smart fertility, preserves 24 hours, fertility rate of egg is still more than 90%.If the various composition consumptions of BJJX liquid are reduced, regulate pH value and osmotic pressure, be mixed with BJJX-II liquid, and by 1: 2 dilution chicken essence liquid, semen deposition immediately, fertility rate of egg is 95.9%, has reached the fertility rate of egg of former smart semen deposition.Therefore, semen deposition immediately after BJJX liquid both had been suitable for diluting also is suitable for doing short-term and preserves the back semen deposition, is a kind of dilution preservation liquid that chicken essence liquid is preserved that is suitable for.Semen deposition immediately after BJJX-II liquid is more suitable for diluting.Artificial insemination during these two kinds of diluents all can be used for raising chickens on a large scale.
Industrial experimentation through carrying out at the Da Xing County, Beijing City animal farm is made to dilute at 1: 2 the B-6(1 of this institute (Institute of Animal Husbandry, China Academy of Agriculture Scinces) with BJJX liquid) be the cock seminal fluid, place 2 ℃ of-5 ℃ of ice chests, be transported to this with automobile by this institute.Went through 6-8 hour, and did conventional semen deposition after transporting 40 kilometers, fertility rate of egg is 72-83%, and average out to 79.9%(is according to laying eggs record, and the laying rate of test hen is 50%).Former smart artificial fertilization's fertility rate of egg is 82.9%.This shows, with the chicken essence liquid of BJJX liquid dilution, not only can make short-term at low temperatures and preserve, also be suitable for short-distance transport, be breeding station exchange chicken essence liquid, improving artificial insemination efficient and reducing chicken raising cost provides convenience.
Connect the industrial experimentation that hollow Wang Wenyuan chicken house carries out according to the west suburb in Beijing horse again, the B-4(1 of this institute with the dilution of BJJX liquid) be that the cock seminal fluid is given the hen semen deposition, fertility rate of egg average out to 91.1% has reached this level with the fertility rate of egg of former polyspermism (90-95%).
Use immediately after BJJX liquid can dilute as can be seen in sum, also use after short-term preservation or the short-distance transport at low temperatures, and the solution preparation is simple, various compositions are obtained easily.
According to fertility rate of egg contrast test (seeing Table 2), by 1: 2 dilution chicken essence liquid, preserve 7 hours down to the hen semen deposition at 5 ℃, fertility rate of egg through the chicken essence liquid of BJJX liquid dilution is 94.7%, fertility rate of egg through the chicken essence liquid of 638334 liquid dilutions is 93.5%, puts down in writing in description of the invention SU638334 simultaneously, dilutes with 638334 liquid, 2 ℃-4 ℃ preservation semen depositions after 7 hours down, fertility rate of egg just reaches 91.8%; By dilution in 1: 1, preserved 24 hours down at 5 ℃, be 92.2% through the fertility rate of egg of the chicken essence liquid of BJJX liquid dilution, and the fertility rate of egg that dilutes with BPSE liquid is 94.8%.As seen, the effect that chicken essence liquid is preserved in the dilution of BJJX liquid is better than 638334 liquid (preserve chicken essence at low temperatures and can only reach 7 hours), is slightly poorer than BPSE liquid, but does not have significant difference (P＜0.05) on the biostatistics.Yet through measuring the sub-survival index of chicken essence down at 33 ℃, the survival index that dilutes the chicken essence liquid of preserving with BJJX liquid is 4.71, and the survival index of preserving with the dilution of BPSE liquid is 3.51.The effect that the dilution of BJJX liquid is preserved is better than BPSE liquid significantly.BJJX liquid comprises 4 kinds of compositions, and 638334 liquid also comprise 4 kinds, and BPSE liquid contains 8 kinds of compositions.By contrast, BJJX liquid and 638334 liquid are simple than BPSE liquid formulation operations.But the acid-base value of 638334 liquid is stable inadequately.The used composition of BJJX liquid all belongs to reagent, buys easily, and is cheap.638334 liquid in this respect similarly.Prepare 100 milliliters, BJJX liquid need be with 0.07 yuan, and 638334 liquid will be with 0.036 yuan.The two is all simple and easy to do, is convenient to promote.And BPSE liquid formulation operations complexity, and sodium glutamate wherein and TES, not only being difficult to buy, price is also expensive.Prepare 100 ml solns, need 0.53 yuan of cost, limited promotion and application.
Take a broad view of fertility rate of egg, chicken essence survival index in diluent and what and the difficulty or ease applied of cost.BJJX liquid is better than BPSE liquid [notes] and 638334 liquid.
Best mode: get glucose 1.40 grams, sodium citrate 1.40 grams, potassium dihydrogen phosphate 0.36 gram, sodium hydrogen phosphate 2.40 grams, add water to 100 milliliters, can make.The diluent of making promptly can be used for diluting the semen deposition immediately of chicken essence liquid, also can be used for dilution and preserves semen deposition after 7 or 24 hours.
Citing document
[1]ОПИСАНИЕ ИЗОЬРЕТЕНИЯ К
АВТОРСКОМУ СВИЦЕТЕЛЬСТВУ 638334
[2]Sexton，T.J.Poultry semen extender
U.S.Pat.APPl.US 288，260 18 Dec 1981，APPl.29 July
1981;12PP Avail NTIS Order NO.PAT-APPL-6-288260;
CA 1982 96∶83127C
The fertility rate of egg contrast test in [notes] in July, 1985: chicken essence liquid is done dilution in 1: 2 through BJJX liquid or BPSE liquid, and after 24 hours, fertility rate of egg is respectively 79.0(184/233 0-5 ℃ of preservation) and 78.5(197/251).
Claims (3)
1, a kind of solution for dilution of cock semen includes potassium dihydrogen phosphate and distilled water, it is characterized in that adding glucose, sodium citrate and sodium hydrogen phosphate, with survival index and its fertility of maintenance that improves chicken essence.The component content of this diluent is:
Glucose 1.20-1.40 gram
Sodium citrate 1.20-1.40 gram
Potassium dihydrogen phosphate 0.15-0.36 gram
Sodium hydrogen phosphate 2.00-2.40 gram
Adding distil water to 100 milliliter.
2, solution for dilution of cock semen according to claim 1 is characterized in that component content is:
Glucose 1.40 grams
Sodium citrate 1.40 grams
Potassium dihydrogen phosphate 0.36 gram
Sodium hydrogen phosphate 2.40 grams
Adding distil water to 100 milliliter
3, solution for dilution of cock semen according to claim 1 is characterized in that component content is:
Glucose 1.20 grams
Sodium citrate 1.20 grams
Potassium dihydrogen phosphate 0.16 gram
Sodium hydrogen phosphate 2.08 grams
Adding distil water to 100 milliliter
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86101836 CN1012375B (en) | 1986-03-22 | 1986-03-22 | Solution for dilution of cock semen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86101836 CN1012375B (en) | 1986-03-22 | 1986-03-22 | Solution for dilution of cock semen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN86101836A true CN86101836A (en) | 1987-01-24 |
| CN1012375B CN1012375B (en) | 1991-04-17 |
Family
ID=4801527
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 86101836 Expired CN1012375B (en) | 1986-03-22 | 1986-03-22 | Solution for dilution of cock semen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1012375B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1313061C (en) * | 2002-03-11 | 2007-05-02 | 刘凤鸣 | Sex sorting method of animal sperma |
| CN103070162A (en) * | 2013-01-30 | 2013-05-01 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
| CN103843759A (en) * | 2014-03-07 | 2014-06-11 | 四川农业大学 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
| CN107637589A (en) * | 2017-11-21 | 2018-01-30 | 广东中农联生物制药有限公司 | Diluent of Pig's Spermatic Fluid and its production method |
| CN108566945A (en) * | 2017-06-26 | 2018-09-25 | 常州市四季禽业有限公司 | Yellow-feather broiler semen diluent and its preparation and application |
| CN108624552A (en) * | 2018-03-27 | 2018-10-09 | 中国农业科学院北京畜牧兽医研究所 | A method of obtaining high-purity chicken sperm |
| CN108852553A (en) * | 2018-07-10 | 2018-11-23 | 江苏现代畜牧科技园 | A kind of artificial semenpicking of goose and semen deposition method |
| CN114794082A (en) * | 2022-04-16 | 2022-07-29 | 贵州省种畜禽种质测定中心 | Antifreeze protein-containing diluent for improving preservation quality of chicken essence and preparation method thereof |
-
1986
- 1986-03-22 CN CN 86101836 patent/CN1012375B/en not_active Expired
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1313061C (en) * | 2002-03-11 | 2007-05-02 | 刘凤鸣 | Sex sorting method of animal sperma |
| CN103070162A (en) * | 2013-01-30 | 2013-05-01 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
| CN103070162B (en) * | 2013-01-30 | 2014-10-08 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
| CN103843759A (en) * | 2014-03-07 | 2014-06-11 | 四川农业大学 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
| CN103843759B (en) * | 2014-03-07 | 2015-05-20 | 四川农业大学 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
| CN108566945A (en) * | 2017-06-26 | 2018-09-25 | 常州市四季禽业有限公司 | Yellow-feather broiler semen diluent and its preparation and application |
| CN107637589A (en) * | 2017-11-21 | 2018-01-30 | 广东中农联生物制药有限公司 | Diluent of Pig's Spermatic Fluid and its production method |
| CN108624552A (en) * | 2018-03-27 | 2018-10-09 | 中国农业科学院北京畜牧兽医研究所 | A method of obtaining high-purity chicken sperm |
| CN108624552B (en) * | 2018-03-27 | 2020-11-27 | 中国农业科学院北京畜牧兽医研究所 | Method for obtaining high-purity chicken sperms |
| CN108852553A (en) * | 2018-07-10 | 2018-11-23 | 江苏现代畜牧科技园 | A kind of artificial semenpicking of goose and semen deposition method |
| CN108852553B (en) * | 2018-07-10 | 2020-06-23 | 江苏现代畜牧科技园 | Artificial semen collection and insemination method for geese |
| CN114794082A (en) * | 2022-04-16 | 2022-07-29 | 贵州省种畜禽种质测定中心 | Antifreeze protein-containing diluent for improving preservation quality of chicken essence and preparation method thereof |
| CN114794082B (en) * | 2022-04-16 | 2022-10-18 | 贵州省种畜禽种质测定中心 | Antifreeze protein-containing diluent for improving preservation quality of chicken essence and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1012375B (en) | 1991-04-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rothstein et al. | The enzymology of the cell surface | |
| Hoffmann et al. | An integrated approach to environmental stress tolerance and life-history variation: desiccation tolerance in Drosophila | |
| Homme et al. | Avian influenza virus infections. I. Characteristics of influenza A/Turkey/Wisconsin/1966 virus | |
| Beckman et al. | Egg production by Acartia tonsa in Long Island sound | |
| CN86101836A (en) | Solution for dilution of cock semen | |
| Skjoldal et al. | Ecobiochemical studies on the deep-water pelagic community of Korsfjorden, Western Norway. Adenine nucleotides in zooplankton | |
| Sexton | Studies on the dilution of turkey semen | |
| Griffith et al. | Serum composition of the coelacanth, Latimeria chalumnae Smith | |
| Bootwalla et al. | Development of diluents for domestic fowl semen | |
| CN112314592A (en) | Egg chicken semen diluent and diluting method | |
| Goodall et al. | Factors affecting the activation and duration of motility of the spermatozoa of the summer whiting (Sillago ciliata) | |
| Rogers | Nitrogen catabolism in nematode parasites | |
| Thomson et al. | Elucidation of the mechanism responsible for the temperature‐dependent reversible inactivation of the motility of fowl spermatozoa | |
| CN102480931A (en) | Sperm diluent solution and method for artificial insemination using same | |
| Wang et al. | Biochemical and physicochemical characteristics of muscle and natural actomyosin isolated from young Atlantic salmon (Salmo Salar) fillets stored at 0 and 4 C | |
| Sexton | Effect of various cryoprotective agents on the viability and reproductive efficiency of chicken spermatozoa | |
| JP3364680B2 (en) | Diluent for swine semen storage and dilution of swine semen | |
| Packard et al. | Water relations and nitrogen excretion in embryos of the oviparous snake Coluber constrictor | |
| Saari et al. | Cleavage of S-(1, 2-dichlorovinyl)-L-cysteine by Escherichia coli B | |
| Billard et al. | The amino acid composition of rainbow trout (Salmo gairdneri) seminal fluid and blood plasma: a comparison with carp (Cyprinus carpio) | |
| CN1232634C (en) | Diluent for sperm of blue fox | |
| Buxton et al. | Enzymes and electrolytes in the semen of Japanese quail | |
| Adebisi et al. | Comparative effect of saline solutions as diluents on in vitro semen storage, egg fertility and hatchability in Turkey hens | |
| ES2907760T3 (en) | Polypeptide sperm stabilizer for semen used in artificial insemination | |
| Zhang et al. | Glycine betaine improves survival of fresh bovine spermatozoa |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C13 | Decision | ||
| C14 | Grant of patent or utility model | ||
| C19 | Lapse of patent right due to non-payment of the annual fee |