CN1012375B - Solution for dilution of cock semen - Google Patents
Solution for dilution of cock semenInfo
- Publication number
- CN1012375B CN1012375B CN 86101836 CN86101836A CN1012375B CN 1012375 B CN1012375 B CN 1012375B CN 86101836 CN86101836 CN 86101836 CN 86101836 A CN86101836 A CN 86101836A CN 1012375 B CN1012375 B CN 1012375B
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- liquid
- semen
- dilution
- cock
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention belongs to a dilute solution for cock semen, which contains dextrose, sodium citrate, monopotassium phosphate, disodium hydrogen phosphate and distilled water. In the present invention, the cock semen is diluted in the proportion of 1: 2 and 1: 1, the diluted cock semen is transported after being respectively preserved for 7 and 24 hours at a temperature of 5 DEG C, and the fertilization rate of eggs respectively are 94.7 % and 92.2%, and has no obvious difference from the fertilization rate (95.5%) of eggs immediately inseminated by original semen; if the dosage of the cock semen is reduced, and the cock semen is diluted in the proportion of 1: 2 and immediately transported, the fertilization rate of the eggs is 95.9%, which achieves the fertilization ability of the original semen. The present invention is suitable for immediate insemination after the cock semen is diluted, and for the insemination after the diluted semen is preserved for a short period and transported within a short distance, and has the advantages of little solution composition, simple preparation, easy obtainment of material, low cost and easy popularization.
Description
The invention belongs to the poultry semen diluent, particularly belong to solution for dilution of cock semen.
Along with the expansion of the scale of raising chickens and the utilization of captive management technology, the technology of artificial insemination of chicken is extensively carried out in poultry husbandry.For improving utilization ratio and the test-tube working efficiency of kind of cock, many countries are in the chickens' extract liquid semen deposition of adopting through diluted.The ideal diluent is not make chickens' extract forfeiture fertility, draws materials easily, and preparation is simple, and cost is low, is convenient to promote.Be mixed with such diluent, be pursuing one's goal of poultry breeding man in decades always.Russian patent SU638334 diluent (hereinafter to be referred as 638334 liquid) is arranged in this respect.This liquid is by containing anhydrous medical glucose 3.0 grams in 100 ml distilled waters, sodium-acetate 1.2 grams, sodium bicarbonate 1.5 grams and 1.5 milliliters of 10% acetic acid are formulated, after being used to dilute chickens' extract liquid, 2 ℃-4 ℃ preservation semen depositions after 7 hours down, fertility rate of egg just reaches 91.8%, does not see that the semen deposition result after its preservation longer time reports, and finds the potential of hydrogen instability of this liquid in preparation.Report according to Chemical Abstracts 1982 96:83127C, the BPSE diluent of the U.S. can make phosphoric acid hydrogen dipotassium 12.70 grams in 900 ml distilled waters, Sodium Glutamate 8.67 grams, fructose 5 grams, sodium-acetate 4.3 grams, TES1.95 gram, Tripotassium Citrate 0.64 gram, potassium primary phosphate 0.65 gram and magnesium chloride 0.34 gram increase distilled water again, and osmotic pressure is transferred to 350 millimole concentration/kg of water.It is generally acknowledged, BPSE liquid is a kind of diluent preferably of dilution chickens' extract liquid, but wherein employed Sodium Glutamate and TES(N-trishydroxymethyl-methyl-2-aminoethane sulphonic acid is the biological buffer that a kind of potential of hydrogen scope is PH6.8-8.2), there is not home products at present, necessary import, it is very inconvenient to buy, and agents useful for same is of a great variety, wherein magnesium chloride reagent is the very strong reagent of a kind of water-absorbent, both cannot say for sure to deposit (very easily deliquescence), and it is accurate to be difficult for weighing again.Cause preparation formality complexity, it is used widely be restricted.To this diluent, except that digest, do not see other relevant documents.Thereby, be difficult to be further described.For this liquid being done further analysis, carried out simultaneous test at the present invention.Find that the survival index of its chickens' extract is not high enough with this liquid dilution chickens' extract liquid.
For overcoming above-mentioned shortcoming, carried out the present invention.The objective of the invention is to improve the survival index of chickens' extract in diluent and keep its normal fertility, and diluent is drawn materials easily, price is cheap, helps promotion and application.For this reason, in containing the fowl semen diluent of potassium primary phosphate and distilled water, replenish adding glucose, Trisodium Citrate and Sodium phosphate dibasic.Dextrose components wherein be sperm at external metabolic energy derive, phosphoric acid salt is buffering system, makes the dilution seminal fluid keep certain potential of hydrogen and buffering ability, Trisodium Citrate is kept the osmotic equilibrium of diluent environment and the interior environment of sperm.The content of every composition is in the diluent:
Glucose 1.20-1.40 gram
Trisodium Citrate 1.20-1.40 gram
Potassium primary phosphate 0.15-0.40 gram
Sodium phosphate dibasic 2.00-2.40 gram
Distilled water adds to 100 milliliters
PH 7.10-7.50
Osmotic pressure 300-370mos/kg
Suitable diluent is named as BJJX liquid, and its component content is:
Glucose 1.40 grams
Trisodium Citrate 1.40 grams
Potassium primary phosphate 0.36 gram
Sodium phosphate dibasic 2.40 grams
Distilled water adds to 100 milliliters
PH 7.5
Osmotic pressure 365mos/kg
The content of above-mentioned diluent can also reduce, and names to be BJJX-II liquid, and every component content is as follows:
Glucose 1.20 grams
Trisodium Citrate 1.20 grams
Potassium primary phosphate 0.16 gram
Sodium phosphate dibasic 2.08 grams
Distilled water adds to 100 milliliters
PH 7.45
Osmotic pressure 310mos/kg
The present invention prepares simply, and content adds mentioned component in accordance with regulations, and adding distil water again is made into 100 ml solns.
Preserve test (as shown in table 1) according to chickens' extract liquid, in 33 ℃ of waters bath with thermostatic control, the survival index average out to 4.71 of its chickens' extract occupies first of each group of test with the chickens' extract liquid of BJJX liquid dilution, survival index than the former essence of not diluted is high by 1.87, and the survival index of former essence is 2.84.Illustrate that BJJX liquid is suitable for the existence of chickens' extract, can be used for preserving chickens' extract liquid.The method of calculation of survival index are: after will diluting chickens' extract liquid and placing above-mentioned water-bath, every one hour observation vigor once, maybe can find out between group till the difference to whole sperm death.During statistics, above-mentioned vigor and this vigor sum are removed with two, multiply by the summation in twice timed interval again, promptly get survival index.
According to fertility rate of egg simultaneous test (as table 2),, under 5 ℃, to preserve and gave the hen semen deposition in 7 hours by 1: 2 dilution chickens' extract liquid with BJJX liquid, fertility rate of egg is 94.7%; Be diluted in 5 ℃ by 1: 1 and preserved 24 hours down, fertility rate of egg is 92.2%, and by dilution in 1: 2, fertility rate of egg was 90.6%; And with the semen deposition immediately of former essence, fertility rate of egg is 95.5%.Illustrate with the dilution of BJJX liquid and preserved at low temperatures 7 hours that fertility rate of egg can reach bright smart fertility, preserves 24 hours, fertility rate of egg is still more than 90%.If the various composition consumptions of BJJX liquid are reduced, regulate pH value and osmotic pressure, be mixed with BJJX-II liquid, and by 1: 2 dilution chickens' extract liquid, semen deposition immediately, fertility rate of egg is 95.9%, has reached the fertility rate of egg of former smart semen deposition.Therefore, semen deposition immediately after BJJX liquid both had been suitable for diluting also is suitable for doing short-term and preserves the back semen deposition, is a kind of dilution preservation liquid that chickens' extract liquid is preserved that is suitable for.Semen deposition immediately after BJJX-II liquid is more suitable for diluting.Artificial insemination during these two kinds of diluents all can be used for raising chickens on a large scale.
Several diluents of table 1. are preserved the test of chickens' extract 33 ℃ of waters bath with thermostatic control
The former essence of BJJX liquid 7-2 liquid BPSE liquid
The first time 4.49 4.26 3.26 3.16
The second time 4.56 4.07 3.21 2.46
For the third time 5.09 4.72 4.05 3.12
Average 4.71 4.35 3.51 2.84
Industrial experimentation through carrying out at the Da Xing County, Beijing City stock-farms, make to dilute at 1: 2 the B-6(1 of this institute (herding institute of the Chinese Academy of Agricultural Sciences) with BJJX liquid) be the cock seminal fluid, place 2-5 ℃ of ice chest, be transported to this with automobile by this institute, went through 6-8 hour, and did conventional semen deposition after transporting 40 kilometers, fertility rate of egg is 72-83%, average out to 79.9%(is according to laying eggs record, and the laying rate of test hen is 50%).The test-tube fertility rate of egg of former essence is 82.9%.This shows, with the chickens' extract liquid of BJJX liquid dilution, not only can make short-term at low temperatures and preserve, also be suitable for short distances transportation, be breeding station exchange chickens' extract liquid, improving artificial insemination efficient and reducing chicken raising cost provides convenience.
Connect the industrial experimentation that hollow Wang Wenyuan chicken house carries out according to the west suburb in Beijing horse again, the B-4(1 of this institute with the dilution of BJJX liquid) be that the cock seminal fluid is given the hen semen deposition, fertility rate of egg average out to 91.1% has reached this level with the fertility rate of egg of former polyspermy (90-95%).
In sum, use immediately after BJJX liquid can dilute as can be seen, also use after short-term preservation or the short distances transportation at low temperatures, and the solution preparation is simple, various compositions are obtained easily.
According to fertility rate of egg simultaneous test (seeing Table 2), by 1: 2 dilution chickens' extract liquid, preserve 7 hours down to the hen semen deposition at 5 ℃, fertility rate of egg through the chickens' extract liquid of BJJX liquid dilution is 94.7%, fertility rate of egg through the chickens' extract liquid of 638334 liquid dilutions is 93.5%, puts down in writing in description of the invention SU638334 simultaneously, dilutes with 638334 liquid, 2-4 ℃ of preservation semen deposition after 7 hours down, fertility rate of egg just reaches 91.8%; By dilution in 1: 1, preserved 24 hours down at 5 ℃, be 92.2% through the fertility rate of egg of the chickens' extract liquid of BJJX liquid dilution, and the fertility rate of egg that dilutes with BPSE liquid is 94.8%.As seen, the effect that chickens' extract liquid is preserved in the dilution of BJJX liquid is better than 638334 liquid (preserve chickens' extract at low temperatures and can only reach 7 hours), is slightly poorer than BPSE liquid, but no KOTK shows difference (P＜0.05) on biostatistics.Yet, measure down the sub-survival index of chickens' extract through 33 ℃, the survival index of the chickens' extract liquid of preserving with the dilution of BJJX liquid is 4.71, and is 3.51 with the survival index that BPSE liquid dilutes preservation.The effect that the dilution of BJJX liquid is preserved obviously is better than BPSE liquid.BJJX liquid comprises 4 kinds of compositions, and 638334 liquid also comprise 4 kinds, and BPSE liquid contains 8 kinds of compositions.By contrast, BJJX liquid and 638334 liquid are simple than BPSE liquid formulation operations.But the potential of hydrogen of 638334 liquid is stable inadequately.The used composition of BJJX liquid all belongs to reagent, buys easily, and is cheap.638334 liquid are in this respect similarly prepared 100 milliliters, and BJJX liquid need be with 0.07 yuan, and 638334 liquid will be with 0.036 yuan.The two is all simple and easy to do, is convenient to promote, and BPSE liquid formulation operations complexity, and Sodium Glutamate wherein and TES, not only being difficult to buy, price is also expensive.Prepare 100 ml solns, need 0.53 yuan of cost, limited and promoted the use of.
Take a broad view of fertility rate of egg, the survival index of chickens' extract in diluent and what and the difficulty or ease of applying of cost.BJJX liquid is better than BPSE liquid
1.With 638334 liquid.
Best mode: get glucose 1.40 grams, Trisodium Citrate 1.40 grams, potassium primary phosphate 0.36 gram, Sodium phosphate dibasic 2.40 grams, add water to 100 milliliters, can make.The diluent of making promptly can be used for diluting the semen deposition immediately of chickens' extract liquid, also can be used for dilution and preserves semen deposition after 7 or 24 hours.
1. the fertility rate of egg comparative test result in July, 1985: chickens' extract liquid is done dilution in 1: 2 through BJJX liquid or BPSE liquid, 0-5 ℃ preserve 24 hours after, fertility rate of egg is respectively 79.0%(184/233) and 78.5%(197/251).
Claims (3)
1, a kind of solution for dilution of cock semen includes potassium primary phosphate and distilled water, it is characterized in that adding glucose, Trisodium Citrate and Sodium phosphate dibasic, with survival index and its fertility of maintenance that improves chickens' extract.The component content of this diluent is:
Glucose 1.20-1.40 gram
Trisodium Citrate 1.20-1.40 gram
Potassium primary phosphate 0.15-0.36 gram
Sodium phosphate dibasic 2.00-2.40 gram
Adding distil water to 100 milliliter
2, solution for dilution of cock semen according to claim 1 is characterized in that component content is:
Glucose 1.40 grams
Trisodium Citrate 1.40 grams
Potassium primary phosphate 0.36 gram
Sodium phosphate dibasic 2.40 grams
Adding distil water to 100 milliliter
3, solution for dilution of cock semen according to claim 1 is characterized in that, component content is
Glucose 1.20 grams
Trisodium Citrate 1.20 grams
Potassium primary phosphate 0.16 gram
Sodium phosphate dibasic 2.08 grams
Adding distil water to 100 milliliter
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86101836 CN1012375B (en) | 1986-03-22 | 1986-03-22 | Solution for dilution of cock semen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86101836 CN1012375B (en) | 1986-03-22 | 1986-03-22 | Solution for dilution of cock semen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN86101836A CN86101836A (en) | 1987-01-24 |
| CN1012375B true CN1012375B (en) | 1991-04-17 |
Family
ID=4801527
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 86101836 Expired CN1012375B (en) | 1986-03-22 | 1986-03-22 | Solution for dilution of cock semen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1012375B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1313061C (en) * | 2002-03-11 | 2007-05-02 | 刘凤鸣 | Sex sorting method of animal sperma |
| CN103070162B (en) * | 2013-01-30 | 2014-10-08 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
| CN103843759B (en) * | 2014-03-07 | 2015-05-20 | 四川农业大学 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
| CN108566945A (en) * | 2017-06-26 | 2018-09-25 | 常州市四季禽业有限公司 | Yellow-feather broiler semen diluent and its preparation and application |
| CN107637589A (en) * | 2017-11-21 | 2018-01-30 | 广东中农联生物制药有限公司 | Diluent of Pig's Spermatic Fluid and its production method |
| CN108624552B (en) * | 2018-03-27 | 2020-11-27 | 中国农业科学院北京畜牧兽医研究所 | Method for obtaining high-purity chicken sperms |
| CN108852553B (en) * | 2018-07-10 | 2020-06-23 | 江苏现代畜牧科技园 | Artificial semen collection and insemination method for geese |
| CN114794082B (en) * | 2022-04-16 | 2022-10-18 | 贵州省种畜禽种质测定中心 | Antifreeze protein-containing diluent for improving preservation quality of chicken essence and preparation method thereof |
-
1986
- 1986-03-22 CN CN 86101836 patent/CN1012375B/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CN86101836A (en) | 1987-01-24 |
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