CN108852553B - Artificial semen collection and insemination method for geese - Google Patents
Artificial semen collection and insemination method for geese Download PDFInfo
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- CN108852553B CN108852553B CN201810750152.1A CN201810750152A CN108852553B CN 108852553 B CN108852553 B CN 108852553B CN 201810750152 A CN201810750152 A CN 201810750152A CN 108852553 B CN108852553 B CN 108852553B
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- 210000000582 semen Anatomy 0.000 title claims abstract description 69
- 230000009027 insemination Effects 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000272517 Anseriformes Species 0.000 title abstract 4
- 241000272814 Anser sp. Species 0.000 claims abstract description 60
- 241000272808 Anser Species 0.000 claims description 16
- 210000003555 cloaca Anatomy 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 10
- 239000003085 diluting agent Substances 0.000 claims description 7
- 238000007689 inspection Methods 0.000 claims description 7
- 210000003746 feather Anatomy 0.000 claims description 6
- 210000003899 penis Anatomy 0.000 claims description 6
- 238000012549 training Methods 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 4
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 4
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 4
- 229960002635 potassium citrate Drugs 0.000 claims description 4
- 239000001508 potassium citrate Substances 0.000 claims description 4
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 claims description 4
- 235000011082 potassium citrates Nutrition 0.000 claims description 4
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 4
- 229940073490 sodium glutamate Drugs 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 3
- 210000001015 abdomen Anatomy 0.000 claims description 3
- 210000000436 anus Anatomy 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 210000001215 vagina Anatomy 0.000 claims description 3
- KEQXNNJHMWSZHK-UHFFFAOYSA-L 1,3,2,4$l^{2}-dioxathiaplumbetane 2,2-dioxide Chemical compound [Pb+2].[O-]S([O-])(=O)=O KEQXNNJHMWSZHK-UHFFFAOYSA-L 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 230000000630 rising effect Effects 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims 1
- 238000005516 engineering process Methods 0.000 claims 1
- 230000001850 reproductive effect Effects 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 abstract description 2
- 230000008030 elimination Effects 0.000 abstract 1
- 238000003379 elimination reaction Methods 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 7
- 230000012447 hatching Effects 0.000 description 7
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 230000013011 mating Effects 0.000 description 6
- 235000013601 eggs Nutrition 0.000 description 4
- 230000008021 deposition Effects 0.000 description 3
- 210000002414 leg Anatomy 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008451 emotion Effects 0.000 description 2
- 210000004394 hip joint Anatomy 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 208000009701 Embryo Loss Diseases 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000008011 embryonic death Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/021—Apparatus for collecting seminal fluids; Artificial vaginas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/027—Devices for injecting semen into animals, e.g. syringes, guns, probes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/027—Devices for injecting semen into animals, e.g. syringes, guns, probes
- A61D19/028—Supporting means thereof, e.g. saddles or belts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Reproductive Health (AREA)
- Public Health (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Media Introduction/Drainage Providing Device (AREA)
- Surgical Instruments (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the technical field of animal feeding, and relates to an artificial semen collection and insemination method for geese. The method for artificially collecting the semen of the male goose and artificially inseminating the female goose is used for reducing the feeding amount of the male goose, delaying the elimination time of the male goose, improving the reproductive performance of the female goose and improving the utilization life of the female goose. Thereby reducing the feeding cost of the geese and improving the economic benefit of the geese.
Description
Technical Field
The invention belongs to the technical field of animal feeding, and particularly relates to an artificial semen collection and insemination method for geese.
Background
On one hand, the existing poultry has higher breeding cost of the male geese in the breeding process, and the male geese are kept less by the poultry in the breeding process through artificial semen collection and semen deposition, and the death rate of embryos is reduced in the hatching process. On the other hand, because the goose body is large, the reproduction rate is low and the brooding performance is extremely strong, the traditional poultry semen collection and insemination method can not meet the insemination requirement of the goose, and the existing artificial semen collection and insemination method needs to be improved.
Disclosure of Invention
The invention aims to provide an artificial semen collection and insemination method for geese, and aims to solve the problems of high cost, low reproduction rate and high embryo death rate in the hatching process of natural mating of breeding male geese.
The invention provides an artificial semen collection and insemination method for geese, which comprises the steps of screening artificially collected male goose semen, diluting the semen by 9 times by using diluent, immediately putting the diluted semen into a temperature regulator, gradually reducing the temperature of the temperature regulator from room temperature to 5-7 ℃, preserving the temperature for 8-10min, gradually heating the temperature to room temperature, taking out the semen and performing artificial insemination on female geese, wherein the diluent is mixed solution consisting of potassium citrate, sodium glutamate, disodium hydrogen phosphate, sodium dihydrogen phosphate, glucose and distilled water according to the mass ratio of 14:142:97:23:75: 10000.
Preferably, the temperature reduction rate of the temperature regulator is 0.5-1 ℃/min.
Preferably, the temperature rising rate of the temperature regulator is 1-1.5 ℃/min.
Preferably, the artificial insemination method comprises the steps that a male goose for artificial insemination is fed in a single cage with strong libido, insemination training is started after 5-7 days of isolation, feathers around a male goose reproductive cavity are cut clean before the insemination training, a shielding device is adopted in the insemination cage, one female goose for test is used, two legs of the female goose for test are fixedly placed in the male goose cage through cotton strips, when the male goose bites the head of the female goose through a mouth and frequently swings tail feathers, and meanwhile, the base part of a penis is exposed out of an anus, an insemination cup is held to lean into the reproductive cavity of the male goose, the hip joint of the male goose is squeezed to enable the penis to be turned out, semen is collected into the insemination cup, and preferably, the insemination training lasts for 5-7 days and then the insemination work is carried out after 5-7 days.
Preferably, the ratio of male to female is 1: 15-20; and the male goose adopts semen for 5 days in one week, and can continuously adopt semen for 5 days, and rest for 2 days or 2 days.
Preferably, the screening comprises semen appearance inspection and semen volume inspection, wherein the semen appearance inspection is to inspect whether the color of the semen is normal, the normal non-polluted semen is milk white and is opaque liquid, and the mixed blood is red and is polluted by the excrement to be yellow; the semen quantity inspection adopts a measuring device such as a pipette gun to suck the semen and measure the primary ejaculation quantity, the ejaculatory quantity is greatly changed along with the age, the season, the individual difference and the proficiency of semen collection operation, the average ejaculatory quantity of the gosling is 0.2-2 ml, and the gosling with more ejaculatory quantity and stability and normal use is selected.
Preferably, the insemination method comprises the steps of pressing the female goose on the insemination platform to form a natural mating squat shape, pressing the back of the female goose to enable the insemination platform to be combined with the abdomen of the female goose, pressing the lower edge of the cloaca, poking the cloaca opening, enabling the cloaca to be opened and turned outwards to the visible vaginal opening, and aligning the tail end of the insemination device conduit with the vagina to insert for insemination.
Preferably, the female goose is in the laying period.
By optimizing the methods for semen collection and semen deposition of the geese, the invention solves the problems of high cost, low reproduction rate and high embryonic death rate of natural mating in the hatching process. Aiming at the problem that the processing capacity of the diluent in the prior art on the semen currently used by the geese is insufficient, the invention screens the components of the semen diluent according to the physiological characteristics of the geese, and finds that the mixed solution consisting of potassium citrate, sodium glutamate, disodium hydrogen phosphate, sodium dihydrogen phosphate, glucose and distilled water according to a specific proportion is suitable for the semen characteristics of the geese.
Detailed Description
Example 1
Materials and methods
1. Test time and mode
1.1 the male goose and the female goose are separated for 5 to 7 days.
1.2 two modes of artificial semen collection, semen deposition and natural mating.
2. Artificial semen collection method
The artificial semen collection gosling is fed in a single cage with strong libido, semen collection can be started after 5-7 days of isolation, feathers around the cloaca of the gosling are cut clean before semen collection, a shielding device is adopted for the semen collection cage, and semen collectors cannot be seen by the gosling during semen collection. When semen is collected, one female goose with test emotion is used, the two legs of the female goose with test emotion are fixed by cotton strips and placed into a male goose cage, when the male goose bites the head of the female goose with a mouth, tail feathers frequently swing, and meanwhile, the base part of a penis begins to be exposed out of an anus, a semen collector leans against a cloaca of the male goose by using a right hand to hold a semen collecting cup, a left hand slightly extrudes at a hip joint of the male goose, the semen collecting cup is taken by a right hand to enable the mouth of the semen collecting cup to abut against the cloaca of the female goose, and the force is exerted upwards to help the penis of the male goose to overturn out, and semen is collected into the semen collecting cup.
3. Semen dilution method
The goose semen is diluted by special diluent (each 100 ml of distilled water contains 0.14g of potassium citrate, 1.42g of sodium glutamate, 0.97g of disodium hydrogen phosphate, 0.23g of sodium dihydrogen phosphate and 0.75g of glucose), and the original semen is diluted by 9 times. And immediately putting the diluted semen into a temperature regulator, setting a program to gradually reduce the temperature of the temperature regulator from room temperature to 5 ℃ at a speed of 0.5 ℃/min, storing for 10min, gradually increasing the temperature to room temperature at a speed of 1 ℃/min, and taking out to perform artificial insemination on the female goose.
4. Method for insemination of female goose
Pressing a female goose on an insemination platform, clamping the female goose between two legs by an insemination person to enable the female goose to be in a natural mating and squatting state, pressing the back of the female goose by a left hand and a right hand by the insemination person to enable the insemination platform to be combined with the abdomen of the female goose, slightly pressing the lower edge of a cloaca, slightly poking the cloaca opening by using an index finger to enable the cloaca to be opened, and at the moment, the two small holes can be seen, one small hole on the right side is a rectum opening for discharging excrement and urine, one small hole on the left side is a vaginal opening, and the right hand is vacated to align the tail end of a sperm feeder catheter to be inserted into a vagina for 2-3 cm to carry out insemination.
Comparative example 1
Insemination by natural mating
Comparative example 2
All procedures were substantially the same as in example 1 except that the dilution was 8-fold.
Comparative example 3
All procedures were substantially the same as in example 1 except that the dilution was 10-fold.
Comparative example 4
All procedures were essentially the same as in example 1, except that the diluted semen was not incubated for 5min at 20 ℃ but was directly inseminated.
Except that 20 (male) and 100 (female) are adopted as the test population in the comparative example 1, 10 (male) and 100 (female) are adopted as the test population in the examples 1 and the comparative examples 2-4, and the results of the comparative analysis of the effects of the groups are shown in the table 1 after 30-day grouping tests.
TABLE 1 Effect of different insemination modes on goose reproductive performance
As can be seen from table 1, by adopting the method of the present embodiment, three data, namely hatching egg fertility rate, hatching egg hatchability and fertilized egg hatchability, are significantly superior to those of the comparative example, and thus, the present invention establishes an artificial goose semen collection and insemination method based on the technical points of semen collection and insemination, which effectively improves the hatching rate of the hatching eggs of the geese, improves the utilization efficiency of the breeding male geese, and reduces the breeding cost.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (1)
1. An artificial semen collection and insemination method for geese is characterized in that male goose semen collected manually is diluted by 9 times through diluent after being screened, the diluted semen is immediately placed into a temperature regulator, the temperature of the temperature regulator is gradually reduced from room temperature to 5 ℃, the diluted semen is stored for 10min and then is gradually heated to room temperature, and then the diluted semen is taken out to carry out artificial insemination on female geese, wherein the diluent is mixed liquid consisting of potassium citrate, sodium glutamate, disodium hydrogen phosphate, sodium dihydrogen phosphate, glucose and distilled water according to the mass ratio of 14:142:97:23:75: 10000; the cooling rate of the temperature regulator is 0.5 ℃/min; the temperature rising rate of the temperature regulator is 1 ℃/min; the artificial insemination method comprises the steps that a male goose with artificial insemination is fed in a single cage with strong libido, insemination training is started after 5-7 days of isolation, feathers around a male goose insemination cavity are trimmed firstly before the insemination training, a shielding device is adopted for the insemination cage, one female goose with a test case is used, the two legs of the female goose with the test case are fixedly placed in the male goose cage through cotton strips, when the male goose bites the head of the female goose through a mouth, tail feathers swing frequently, and meanwhile, the base part of a penis is exposed out of an anus, a sperm collecting cup is held to lean against the insemination cavity of the male goose, the joint of the male goose is extruded to enable the penis to turn out, sperm is collected into the sperm collecting cup, the insemination training lasts for 5-7 days, and insemination work is carried out after 5-7 days; the proportion of male to female by adopting the artificial insemination technology is 1: 15-20; and the male goose adopts semen for 5 days in one week, and can continuously adopt semen for 5 days, and then the male goose takes 2 days for rest or takes 2 days for rest for one day; the screening comprises semen appearance inspection and semen volume inspection, wherein the semen appearance inspection is to inspect whether the color of the semen is normal, the normal non-polluted semen is milk white and is opaque liquid, and the mixed blood is red and is polluted by the excrement to be yellow; the semen quantity check comprises the steps of sucking semen by adopting a pipette gun measuring device, measuring the primary ejaculation quantity, wherein the ejaculatory quantity is greatly changed along with the age, season, individual difference and proficiency of semen collection operation, and the average ejaculatory quantity of the gosling is 0.2-2 ml; the insemination method comprises the steps that a female goose is naturally mated and squat on an insemination platform, the back of the female goose is pressed to enable the insemination platform to be combined with the abdomen of the female goose, the lower edge of a cloaca is squeezed, the cloaca opening is poked open, the cloaca is opened and turned outwards to a visible vaginal opening, and the tail end of a duct of a insemination device is aligned with a vagina and inserted for insemination; the female goose is in the egg producing period.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810750152.1A CN108852553B (en) | 2018-07-10 | 2018-07-10 | Artificial semen collection and insemination method for geese |
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| CN201810750152.1A CN108852553B (en) | 2018-07-10 | 2018-07-10 | Artificial semen collection and insemination method for geese |
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| CN108852553A CN108852553A (en) | 2018-11-23 |
| CN108852553B true CN108852553B (en) | 2020-06-23 |
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| CN112630457B (en) * | 2020-12-25 | 2022-12-27 | 河南农业大学 | Method for screening goose wings based on blood biochemical indexes and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86101836A (en) * | 1986-03-22 | 1987-01-24 | 中国农业科学院畜牧研究所 | Solution for dilution of cock semen |
| CN103271779A (en) * | 2013-05-13 | 2013-09-04 | 沅江市湘丰鹅业发展有限公司 | Artificial fertilization method for breeding geese |
| CN103598142A (en) * | 2013-11-16 | 2014-02-26 | 罗平县智龙养鹅专业合作社 | Diandong goose and cultivation method thereof |
| CN105875503A (en) * | 2016-04-29 | 2016-08-24 | 西南大学荣昌校区 | Treatment method for increasing fertilization rate of white goose |
-
2018
- 2018-07-10 CN CN201810750152.1A patent/CN108852553B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86101836A (en) * | 1986-03-22 | 1987-01-24 | 中国农业科学院畜牧研究所 | Solution for dilution of cock semen |
| CN103271779A (en) * | 2013-05-13 | 2013-09-04 | 沅江市湘丰鹅业发展有限公司 | Artificial fertilization method for breeding geese |
| CN103598142A (en) * | 2013-11-16 | 2014-02-26 | 罗平县智龙养鹅专业合作社 | Diandong goose and cultivation method thereof |
| CN105875503A (en) * | 2016-04-29 | 2016-08-24 | 西南大学荣昌校区 | Treatment method for increasing fertilization rate of white goose |
Non-Patent Citations (2)
| Title |
|---|
| 不同稀释液对鸡精液低温保存影响的研究;金美林，等;《甘肃畜牧兽医》;20170831;第47卷(第8期);第87-88页 * |
| 鹅的人工授精技术及应注意的问题;张玉前，等;《畜禽业》;20061231(第212期);第33-35页 * |
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Inventor after: Lu Yanfeng Inventor after: Mu Xiaohui Inventor after: Bian Youqing Inventor after: Duan Xiujun Inventor after: Qin Haorong Inventor after: Ding Yinhua Inventor after: Sun Guobo Inventor after: Li Xiaoming Inventor after: Ji Rongchao Inventor after: Zhang Gansheng Inventor before: Bian Youqing Inventor before: Duan Xiujun Inventor before: Qin Haorong Inventor before: Lu Yanfeng Inventor before: Sun Guobo Inventor before: Li Xiaoming Inventor before: Ji Rongchao Inventor before: Zhang Gansheng Inventor before: Mu Xiaohui |
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