CN110741986A - Artificial propagation method of silurus hamiltonii - Google Patents
Artificial propagation method of silurus hamiltonii Download PDFInfo
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- CN110741986A CN110741986A CN201911132929.9A CN201911132929A CN110741986A CN 110741986 A CN110741986 A CN 110741986A CN 201911132929 A CN201911132929 A CN 201911132929A CN 110741986 A CN110741986 A CN 110741986A
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- 241000347488 Silurus Species 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241000251468 Actinopterygii Species 0.000 claims abstract description 111
- 230000012447 hatching Effects 0.000 claims abstract description 13
- 230000004720 fertilization Effects 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 46
- 238000002347 injection Methods 0.000 claims description 27
- 239000007924 injection Substances 0.000 claims description 27
- 238000011534 incubation Methods 0.000 claims description 25
- 235000013601 eggs Nutrition 0.000 claims description 18
- 210000000582 semen Anatomy 0.000 claims description 18
- 210000001015 abdomen Anatomy 0.000 claims description 12
- 241000238631 Hexapoda Species 0.000 claims description 6
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 claims description 6
- 101800000989 Oxytocin Proteins 0.000 claims description 6
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 claims description 6
- 230000012173 estrus Effects 0.000 claims description 6
- 239000013505 freshwater Substances 0.000 claims description 6
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 claims description 6
- 229960001723 oxytocin Drugs 0.000 claims description 6
- 239000010802 sludge Substances 0.000 claims description 6
- 239000000262 estrogen Substances 0.000 claims description 4
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 2
- 229940011871 estrogen Drugs 0.000 claims 2
- 241000238557 Decapoda Species 0.000 claims 1
- 241000143060 Americamysis bahia Species 0.000 description 5
- 230000001360 synchronised effect Effects 0.000 description 3
- 241000721179 Clarias Species 0.000 description 2
- 230000002196 ecbolic effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 241001233037 catfish Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides an artificial propagation method of silurus hamiltonii, which comprises the steps of parent fish selection, parent fish cultivation and artificial propagation. The method adopts a scientific artificial propagation method to cultivate the silurus littoralis, has high fertilization and hatching rate, solves the problem that the number of wild silurus littoralis is sharply reduced due to over-fishing, and explores to realize the artificial propagation of the silurus littoralis.
Description
Technical Field
The invention belongs to the technical field of artificial fish breeding, and particularly relates to an artificial breeding method of silurus hamiltonii.
Background
The catfish is a Clarias littoralis (G ü enther, 1864)), a fish of Clarias littoralis of Clariaceae is free of dorsal fins, hip fin strips ii-81-84, pectoral fins i-13-16, ventral fins i-7-8, tail fins i-15-i, gill harrow 18-19, body length 240mm is 4.9-5.3 times of body height, head length is 4.6-5.1 times of head length, head length is 2.7-3.3 times of kiss length, eye diameter is 7-8.3 times of body extension, very side flat head is small, wide and longitudinal flat eye is medium large, 149 is located below the front side of the head, front and back nostrils are far apart, mouth cracks are shallow, rear end is not beyond anterior eye edge, jaw is short and thin, rear end exceeds rear edge, chin is beyond anterior eye edge, thin chin is close to the anterior eye edge, thick, lateral opening is not close to the anterior nares, the lateral opening is not connected with the posterior nares, the upper side of the chin is not connected with the lateral side of the chin, the lateral side of the fin, the chin is not more, the posterior branch of the hip fin, the hip fin strips, the posterior branch point, the posterior branch of the hip fin strips, the dorsal fin strips are not more, the posterior branch point, the dorsal fin strips are more the dorsal fin strips, the dorsal strips, the posterior fin strips are not more the posterior fin strips.
Disclosure of Invention
The invention provides an artificial propagation method of silurus hamiltonii, which comprises the following specific technical scheme:
an artificial propagation method of silurus hamiltonii comprises the following steps:
firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection;
secondly, parent fish cultivation:
(1) a cultivation pool:
the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.2h, the square meter is 0.33h, and the water depth is 1.5m to 2 m; the pond clearing is in accordance with SC/T1008-2002.
(2) And (3) male and female matching:
the male and female proportion is as follows: 1: 1;
(3) stocking amount:
the stocking amount of the parent fish is 140kg/100 square meters or the stocking density is 50-100 per 100 square meters;
(4) feeding and feeding:
silurus hamiltonii belongs to carnivorous fishes, and the parent fish feed is mainly palatable live bait; the daily bait feeding amount is 2% -3% of the weight of the fish, and the daily bait feeding amount is adjusted according to seasons and water temperature;
thirdly, artificial propagation:
(1) parent fish selection and matching:
the selection criteria were: the female fish is more than 3+ old, the weight is more than 1.5 kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 1kg, and semen flows out when the abdomen is lightly pressed; the male and female proportion is as follows: 1: 1;
(2) injection amount and method of oxytocin:
the male and female fishes are injected with the multi-oestrogen (Chorionic Gonadothrophin for Injection (I)) at one time, the Injection amount of the female fishes is 1kg/900 unit to 1kg/2100 unit, and the fishes are half of the amount of the female fishes and are synchronously injected at one time; test results show that the injection amount of the oxytocic is 1kg/1200 units to 1kg/1500 units, the fertilization rate reaches more than 90 percent, and the oxytocic is most suitable.
(3) Artificial fertilization:
when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container;
(4) hatching:
and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
Further, in the step two (3), the filter-feeding fishes are put in the parent fish putting-in pond in a matching way.
Further, in the second step (4), the palatable live baits are live fishes, live shrimps and live insects.
Further, the artificial propagation time is 4-7 months per year.
Further, in the third step (4), the hatching in flowing water is performed by adopting an annular flowing water groove.
Further, in the third step (4), the incubation temperature is 22-28 ℃.
The invention has the beneficial effects that: the method has the advantages that the silurus ascheri is cultivated by adopting a scientific artificial propagation method, the fertilization and hatching rate is high, the problem that the number of wild silurus ascheri is sharply reduced due to excessive fishing for economic purposes is solved, and the artificial propagation of the silurus ascheri is explored and realized.
Detailed Description
Example 1:
an artificial propagation method of silurus hamiltonii comprises the following steps:
firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection;
secondly, parent fish cultivation:
(1) a cultivation pool:
the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.2h, and the water depth is 1.5 m; the pond clearing is in accordance with SC/T1008-2002.
(2) And (3) male and female matching:
the male and female proportion is as follows: 1: 1;
(3) stocking amount:
the stocking amount of parent fishes is 140kg/100 square meters or the stocking density is 50 pieces/100 square meters;
(4) feeding and feeding:
silurus hamiltonii belongs to carnivorous fishes, and the parent fish feed is mainly palatable live bait; the daily bait feed amount is 2% of the weight of the fish, and is adjusted according to seasons and water temperature;
thirdly, artificial propagation:
(1) parent fish selection and matching:
the selection criteria were: the female fish is more than 3+ old, the weight is more than 1.5 kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 1kg, and semen flows out when the abdomen is lightly pressed; the male and female proportion is as follows: 1: 1;
(2) injection amount and method of oxytocin:
female and male fish are injected with multivitamin (Chorionic Gonadophophin for Injection (I)) at one time, the Injection amount of female fish is 1kg/900 unit-1 kg/1200 unit, the fish is half of the amount of female fish, and the Injection is performed at one time synchronously;
(3) artificial fertilization:
when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container;
(4) hatching:
and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
Further, in the step two (3), the filter-feeding fishes are put in the parent fish putting-in pond in a matching way.
Further, in the second step (4), the palatable live baits are live fishes, live shrimps and live insects.
Further, the artificial propagation time is 4-7 months per year.
Further, in the third step (4), the hatching in flowing water is performed by adopting an annular flowing water groove.
Further, in the third step (4), the incubation temperature is 22-28 ℃.
The hatchability of the water at 24-26 ℃ is best, and can reach more than 80%.
Example 2:
an artificial propagation method of silurus hamiltonii comprises the following steps:
firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection;
secondly, parent fish cultivation:
(1) a cultivation pool:
the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.2h, and the water depth is 1.5 m; the pond clearing is in accordance with SC/T1008-2002.
(2) And (3) male and female matching:
the male and female proportion is as follows: 1: 1;
(3) stocking amount:
the stocking amount of parent fishes is 140kg/100 square meters or the stocking density is 50 pieces/100 square meters;
(4) feeding and feeding:
silurus hamiltonii belongs to carnivorous fishes, and the parent fish feed is mainly palatable live bait; the daily bait feed amount is 2% of the weight of the fish, and is adjusted according to seasons and water temperature;
thirdly, artificial propagation:
(1) parent fish selection and matching:
the selection criteria were: the female fish is more than 3+ old, the weight is more than 1.5 kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 1kg, and semen flows out when the abdomen is lightly pressed; the male and female proportion is as follows: 1: 1;
(2) injection amount and method of oxytocin:
female and male fish are injected with multivitamin (Chorionic Gonadophophin for Injection (I)) at one time, the Injection amount of female fish is 1kg/1200 unit-1 kg/1500 unit, the fish is half of the amount of female fish, and the synchronous Injection is carried out at one time;
(3) artificial fertilization:
when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container;
(4) hatching:
and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
Further, in the step two (3), the filter-feeding fishes are put in the parent fish putting-in pond in a matching way.
Further, in the second step (4), the palatable live baits are live fishes, live shrimps and live insects.
Further, the artificial propagation time is 4-7 months per year.
Further, in the third step (4), the hatching in flowing water is performed by adopting an annular flowing water groove.
Further, in the third step (4), the incubation temperature is 22-28 ℃.
The hatchability of the water at 24-26 ℃ is best, and can reach more than 80%.
Example 3:
an artificial propagation method of silurus hamiltonii comprises the following steps:
firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection;
secondly, parent fish cultivation:
(1) a cultivation pool:
the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.33h, and the water depth is 2 m; the pond clearing is in accordance with SC/T1008-2002.
(2) And (3) male and female matching:
the male and female proportion is as follows: 1: 1;
(3) stocking amount:
the stocking amount of parent fishes is 140kg/100 square meters or the stocking density is 100 per 100 square meters;
(4) feeding and feeding:
silurus hamiltonii belongs to carnivorous fishes, and the parent fish feed is mainly palatable live bait; the daily bait feed amount is 3% of the weight of the fish, and is adjusted according to seasons and water temperature;
thirdly, artificial propagation:
(1) parent fish selection and matching:
the selection criteria were: the female fish is more than 3+ old, the weight is more than 1.5 kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 1kg, and semen flows out when the abdomen is lightly pressed; the male and female proportion is as follows: 1: 1;
(2) injection amount and method of oxytocin:
female and male fish are injected with multivitamin (Chorionic Gonadophophin for Injection (I)) at one time, the Injection amount of female fish is 1kg/1500 unit-1 kg/1800 unit, the fish is half of the metering amount of the female fish, and the synchronous one-time Injection is carried out;
(3) artificial fertilization:
when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container;
(4) hatching:
and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
Further, in the step two (3), the filter-feeding fishes are put in the parent fish putting-in pond in a matching way.
Further, in the second step (4), the palatable live baits are live fishes, live shrimps and live insects.
Further, the artificial propagation time is 4-7 months per year.
Further, in the third step (4), the hatching in flowing water is performed by adopting an annular flowing water groove.
Further, in the third step (4), the incubation temperature is 22-28 ℃.
The hatchability of the water at 24-26 ℃ is best, and can reach more than 80%.
Example 4:
an artificial propagation method of silurus hamiltonii comprises the following steps:
firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection;
secondly, parent fish cultivation:
(1) a cultivation pool:
the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.33h, and the water depth is 2 m; the pond clearing is in accordance with SC/T1008-2002.
(2) And (3) male and female matching:
the male and female proportion is as follows: 1: 1;
(3) stocking amount:
the stocking amount of parent fishes is 140kg/100 square meters or the stocking density is 100 per 100 square meters;
(4) feeding and feeding:
silurus hamiltonii belongs to carnivorous fishes, and the parent fish feed is mainly palatable live bait; the daily bait feed amount is 3% of the weight of the fish, and is adjusted according to seasons and water temperature;
thirdly, artificial propagation:
(1) parent fish selection and matching:
the selection criteria were: the female fish is more than 3+ old, the weight is more than 1.5 kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 1kg, and semen flows out when the abdomen is lightly pressed; the male and female proportion is as follows: 1: 1;
(2) injection amount and method of oxytocin:
the female and male fishes are injected with the multi-oestrogen (Chorionic Gonadothrophin for Injection (I)) at one time, the Injection amount of the female fishes is 1kg/1800 unit to 1kg/2100 unit, the fish is half of the metering amount of the female fishes, and the synchronous one-time Injection is carried out;
(3) artificial fertilization:
when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container;
(4) hatching:
and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
Further, in the step two (3), the filter-feeding fishes are put in the parent fish putting-in pond in a matching way.
Further, in the second step (4), the palatable live baits are live fishes, live shrimps and live insects.
Further, the artificial propagation time is 4-7 months per year.
Further, in the third step (4), the hatching in flowing water is performed by adopting an annular flowing water groove.
Further, in the third step (4), the incubation temperature is 22-28 ℃.
The hatchability of the water at 24-26 ℃ is best, and can reach more than 80%.
Claims (6)
1. An artificial propagation method of silurus hamiltonii, which is characterized by comprising the following steps: firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection; secondly, parent fish cultivation: (1) a culture pond: the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.2h, the square meter is 0.33h, and the water depth is 1.5m to 2 m; (2) male and female ratio: the male and female proportion is as follows: 1: 1; (3) stocking amount: the stocking amount of the parent fish is 140kg/100 square meters or the stocking density is 50-100 per 100 square meters; (4) feeding and feeding: silurus hamiltonii belongs to carnivorous fishes, and the parent fish feed is mainly palatable live bait; the daily bait feeding amount is 2% -3% of the weight of the fish, and the daily bait feeding amount is adjusted according to seasons and water temperature; thirdly, artificial propagation: (1) parent fish selection and matching: the selection criteria were: the female fish is more than 3+ old, the weight is more than 1.5 kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 1kg, and semen flows out when the abdomen is lightly pressed; the male and female proportion is as follows: 1: 1; (2) injection amount and method of oxytocin: injecting the female and male fish with 1kg/900 units-1 kg/2100 units of estrogen and half of the female fish at one time, and synchronously injecting the estrogen and the male fish at one time; (3) artificial fertilization: when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container; (4) hatching: and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
2. The artificial propagation method of silurus hamiltonii as claimed in claim 1, wherein in step two (3), filter-feeding fishes are bred in the parent fish breeding pond.
3. The artificial propagation method of silurus hamiltonii as claimed in claim 1, wherein in the second step (4), the palatable live baits are live fish, live shrimp, and live insects.
4. The artificial propagation method of silurus hamatu as claimed in claim 1, wherein the time for artificial propagation is 4-7 months per year.
5. The artificial propagation method of silurus hamiltonii as claimed in claim 1, wherein in the third step (4), incubation in running water is performed by using an annular flowing water tank.
6. The artificial propagation method of silurus hamiltonii as claimed in claim 1, wherein in the third step (4), the incubation temperature is 22-28 ℃.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114868676A (en) * | 2022-03-08 | 2022-08-09 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for male meinan silurus meridionalis |
| CN114885865A (en) * | 2022-03-29 | 2022-08-12 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for female silurus riparia |
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| US20100035327A1 (en) * | 2008-08-11 | 2010-02-11 | Ann Marie Steele | Use of rice-derived products in a universal cell culture medium |
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