CN103563848A - Fertilization method for Tibetan chicken - Google Patents
Fertilization method for Tibetan chicken Download PDFInfo
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- CN103563848A CN103563848A CN201310533125.6A CN201310533125A CN103563848A CN 103563848 A CN103563848 A CN 103563848A CN 201310533125 A CN201310533125 A CN 201310533125A CN 103563848 A CN103563848 A CN 103563848A
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Abstract
The invention discloses a fertilization method for Tibetan chicken. The method comprises the following steps: (1) selection of breeding chicken: breeding cocks and breeding hens are selected according to a quantity ratio of 1:(1-20); (2) semen collection of the breeding cocks: semen collection is performed on the breeding cocks in the afternoon, a semen collection cup is used for collecting semen in the copulatory organ position above a cloaca of each cock; and (3) insemination of breeding hens: insemination is performed on the breeding hens in the afternoon, an insemination device filled with the semen is inserted into the vagina of each hen for semen injection, and the hen is placed back to a cage after the insemination is finished. The fertilization method for the Tibetan chicken disclosed by the invention adopts standardized operation, and is not influenced by defects such as unstable fertilization rate, large fluctuation and the like of wild pairing of the Tibetan chicken, mass Tibetan chicken breeding and cultivation with stable and high fertilization rate can be achieved, and the breeding cost is reduced effectively.
Description
Technical field
The present invention relates to a kind of fertilization method, specifically a kind of for hiding the fertilization method of chicken, belong to animal biology field.
Background technology
Hiding chicken is that one way of life is the chicken of pure free of contamination snow-coated plateau, small and exquisite kind the flying of build, the good meat egg dual-purpose type landrace of China, its meat protein content is high, be rich in whole essential amino acids, its content is very similar to the aminogram formula in egg, Ruzhong, is therefore the protein source of high-quality, particularly collagen content is apparently higher than other fowl poultry kind meat product, and nutritious structure is comprehensive.
Hide at present the aquaculture model that chicken cultivates the original backwardness of also raising scattered in peasant household, within its year, the amount of delivering for sale can not meet the needs of national market completely.While, in culture zone, the masses extensively introduced interior ground breeding broiler chicken along with the development of commodity poultry production and the improvement of transportation condition, and the purebred Tibetan of high-quality fowl raising quantity falls sharply.Along the line at primary highway, purebred Tibetan chicken is very rare, hides chicken culturing area more and more less, and this variety source has been absorbed in endangered condition.
It is all the natural insemination mode that adopts groupuscule kind that traditional Tibetan chicken breeds, by natural mating, realize fertilization, but there is many defects in this fertilization mode, mainly comprise: fertilization rate is unstable, fluctuation is large, the fertilization rate height of natural insemination has much relations with the state of male and female chicken, cannot keep stable fertilization rate; Be unfavorable for breed breeding, because natural mating is to take groupuscule as fundamental unit, cannot accomplish the corresponding one by one of cock and hen, cause being difficult to set up pedigree, cannot realize and improve the breed breeding of hiding chicken productivity; Easily cause damage or the pollution of kind of egg.
Summary of the invention
Defect for existing Tibetan chicken fertilization method, the invention discloses a kind of for hiding the fertilization method of chicken, compare with conventional method, it is small that method of the present invention has fully taken into account Tibetan chicken self, cock lack of quantity of spermatic fluid, wild nature is strong, is difficult for the features such as domestication and Tibetan area height above sea level, low, the dry anoxic of temperature etc. and causes the seminal fluid limiting factors such as the time-to-live is short in vitro, vigor is low, whole fertilization process, time etc. are made to specific aim and improve, thereby realized stable, fertilization efficiently; Meanwhile, method of the present invention is not affected by natural conditions and human factor, can realize industrialization, standardization, operation in enormous quantities.
For achieving the above object, the present invention is achieved through the following technical solutions:
For hiding the fertilization method of chicken, comprise the steps: 1) selection of planting chicken: select kind of cock and plant hen, the quantity of the two is than being 1:1-20; 2) plant the semen collection of cock: in the afternoon kind of cock is carried out to semen collection, the copulatory organ place collecting semen with semen collection cup above cock cloaca; 3) plant the semen deposition of hen: in the afternoon kind of hen is carried out to semen deposition, have the inseminating syringe of seminal fluid to insert kind of a hen vagina suction and inject seminal fluid, after semen deposition finishes, kind of a hen is put back in cage.
In said method, the selection of planting the female ratio of cock has fully taken into account that Tibetan chicken is small, body weight is light, plants the little situation of the each volume of semen of cock, in order to guarantee volume of semen and fertilization rate, thereby has selected aforementioned proportion.
Preferably, planting cock and plant the two quantity ratio of hen is 1: 6-10.
Applicant by a large amount of chicken praxiology researchs different time semen collection and the impact of fertilization on final fertilization rate, discovery can obtain the most stable high fertilization rate in following time period semen collection and fertilization: the semen collection time of planting cock is 2:30-3:30 in afternoon, and the semen deposition time of planting hen is 2:00-8:00 in afternoon.
Applicant has further studied the selection of time of fertilization, consider due to egg in uterus the time of staying have 19 hours more than, if egg semen deposition in uterus time will stop the sperm to move upward along oviduct.Sperm just mutually combines when pars infundibularis and ovum meet, and produces fertilization phenomenon.If funnel position does not have the sperm of sufficient amount, fertilization will be affected.Therefore, before ovum enters uterus, semen deposition effect is not best, therefore more preferably 16 of every afternoons, kind of hen is carried out to semen deposition later.Consider the biological property of smart ovum combination, the volume of semen of every kind cock is 0.3-0.6mL; The semen deposition degree of depth of planting hen is that inseminating syringe inserts 1.5cm in vagina, every kind hen semen deposition 0.025-0.03ml.
Therefore in the present invention, kind cock used should be the Tibetan cock chicken that spermatozoon activity is good, the invention also discloses the system of selection of kind of cock, adopts multistage screening, until select meet semen collection demand cock as kind of a cock.
Concrete, comprise following four steps: for the first time, in 1 age in days cock, eliminate small and weak cock; For the second time, the cock that reservation physically well develops in 60 age in days left and right cocks, cockscomb is scarlet; For the third time, in age in 19-21 week, in cock, retain secondary sexual character good, physically fit, have the cock of sexual reflex; The 4th time, in the cock about 24 week age, eliminate aspermia or the poor cock of semen quality, the cock retaining through above-mentioned four screening processes screening is as kind of a cock.
The control that it should be noted that above-mentioned time point need to be considered the impact of cock breeding environment on cock rate of development, so those skilled in the art can rationally adjust the time point of above-mentioned screening according to actual developmental state.
After filtering out kind of cock, the semen collection of carrying out for can be stable, also comprises the step that it is trained, and the kind cock between 180-200 ages in days is trained continuously until semen deposition.
Meanwhile, the kind cock of selecting should remain under good raising condition, preferred, and plant cock and adopt single cage to raise, 16-24 ℃ of hen house temperature, humidity 55%, keeps ventilated, and light application time is constant is 15-16 hours/days.
In concrete operations of the present invention, the semen deposition of planting hen specifically comprises two steps: 1) turn over anus: the cloaca of kind of hen is outwards routed up, exposed the rectum mouth on right side and the introitus in left side; 2) semen deposition: have the inseminating syringe of seminal fluid to insert vagina by inhaling, slowly inject seminal fluid.
Above-mentioned two processes preferably adopt double completing, and increase work efficiency.
By above-mentioned improvement, method of the present invention has realized following effect:
1, make full use of hen house area, increase raising capacity: owing to having realized the reasonable screening of planting cock and planting hen, can reduce invalid raising and waste.
2, improve the utilization ratio of outstanding cock: by screening suitable kind cock, can avoid invalid fertilization.
3, be conducive to control and the purification of disease: aforesaid operations process is standardized work, be not subject to natural environment influence.
4, good economy performance: for feeding separately of kind of cock and kind hen Different Nutrition demand.Reduce feeding cost.
Embodiment
In following, applicant provides a specific implementation of fertilization method of the present invention, the present invention is not formed and is particularly limited.
One, plant selecting of cock:
In population, according to following step, select kind of a cock, for the first time: 1 age in days, eliminate small and weak cock; For the second time: 60 about ages in days, select and remain physically well develop, cock that cockscomb is scarlet; For the third time: in age in 19-21 week, the secondary sexual character of selecting and remain is good, physically fit, has the cock of sexual reflex; The 4th time: about 24 week age, during semen collection training, eliminate aspermia or the poor cock of semen quality.
By said process, filter out suitable cock as kind of a cock, the biological character of planting cock is as follows:
1. outward appearance: physique stalwartness, brawniness, shirtfront is broad, eyes are bright god, active and intelligent, calls limpid; Legs and feet are sturdy, the solid high resilience of foot pad; Full-fledged glossy, non-variegation, secondary sexual character is obvious, and cockscomb and wattle physically well develop, and color is scarlet.
2. productivity: volume of semen is at 0.3-0.6mL, and seminal fluid is sticky, milky.Use electron microscope monitoring sperm concentration and vigor to screen, density is generally 25-40 hundred million/mL, and sperm rectilinear motion, without deformity.
Above-mentioned kind of cock trained, and between 180-200 ages in days, every three days once, and general continuous training four times is until semen deposition.
The raising condition of above-mentioned kind of cock is that single cage is raised, and keeps 16-24 ℃, hen house, and humidity 55% and ventilated good, is beneficial to abundant absorption and the utilization of kind of chicken to nutrition.Light application time is constant is 15-16 hours/days, and specific implementation is one, 10 square metres of 45 watts of bulbs.
Two, preparation before semen collection
Before semen collection, the utensil of semen collection process need is carried out to fumigation; To collection spermaduct, to do pre-temperature (winter: 36-37 ℃, summer: 35-36 ℃) process.3-4 hour should stop eating before the cock semen collection of semen collection.
Three, the semen collection time
Be arranged in 2:30-3:30 kind of cock is carried out to semen collection, then semen deposition.
Four, plant cock semen collection method
Semen collection is spaced apart 3 days.In semen collection process, sperm volume obviously kind cock on the low side is raised separately, and replenishing vitamins and protein, makes its many rest a period of time until its sperm volume recovers normal.
During collecting semen, can not sneak into the impurity pollution seminal fluid such as excrement slag, fine hair, if any polluting, not use.
The seminal fluid gathering is put into preheating in 12 milliliters of test tubes of 39-42 ℃ of warm water, and the semen collection time is preferably no more than 15 minutes.
During semen collection operation, preferably, by the method semen collection of abdomen back and massaging, a people stablizes cock, people's massage and collection seminal fluid, the copulatory organ place collecting semen with semen collection cup above cock cloaca.
Five, plant hen semen deposition method
Plant hen when colony's laying rate reaches 5%, just can carry out artificial insemination.Every hen is every semen deposition in 3 days 1 time, and the semen deposition time carries out after every afternoon, 16:00 laid eggs.Before semen deposition, pick up egg, if any the hen of laying eggs after semen deposition, more once defeated.
In semen deposition when operation,, can be coordinated by two people, and a people grabs chicken and turns over anus, and a people inputs seminal fluid, adopts the semen deposition of turning up of hen introitus.
Turn over anus personnel kind of a hen is mentioned, hand thumb and forefinger, middle finger is in cloaca slightly firmly ventrad extruding around; The left hand of simultaneously grabbing leg pulls back on one side, with middle finger, forefinger in breastbone rear end upwards push up on one side, cloaca is outwards routed up, and inside has two openings, and right side is rectum mouth, and left side is introitus.Then semen deposition personnel have the inseminating syringe of seminal fluid to insert vagina by inhaling, and slowly inject seminal fluid.Meanwhile, assistant's right hand slowly unclamps, in case seminal fluid benefit.Note not by air or bubble input oviduct, otherwise will affect fertilization rate.Semen deposition finishes, and hen is put back in cage.
The semen deposition degree of depth is that inseminating syringe is inserted to 1.5cm left and right in vagina, and every hen only need to be inputted 0.025-0.03ml.
In actual use, applicant uses respectively 10 kind cocks and 60 kind hens, 10 kind cocks and 70 kind hens, 10 kind cocks and 90 kind hens, 10 kind cocks and 100 kind hens as four experimental group, the insemination dose adopting is followed successively by 0.03ml, 0.028ml, 0.027ml, 0.025ml, the semen collection time is respectively 2:30 in afternoon, 2:30,3:00,3:30, the semen deposition time is respectively 3:00 in afternoon, 4:00,4:30,5:00, and the semen deposition degree of depth is 1.5cm in vagina.
Experimental result shows, the fertilization rate of every group is all higher than 90%, and wherein second and the 3rd experimental group are fertilized successful interest rate higher than 95%, illustrate that fertilization method of the present invention is a kind of effective fertilization method.
Claims (10)
1. for hiding the fertilization method of chicken, it is characterized in that comprising the steps: 1) selection of planting chicken: select kind of cock and plant hen, the quantity of the two is than being 1:1-20; 2) plant the semen collection of cock: in the afternoon kind of cock is carried out to semen collection, the copulatory organ place collecting semen with semen collection cup above cock cloaca; 3) plant the semen deposition of hen: in the afternoon kind of hen is carried out to semen deposition, have the inseminating syringe of seminal fluid to insert kind of a hen vagina suction and inject seminal fluid, after semen deposition finishes, kind of a hen is put back in cage.
2. fertilization method according to claim 1, is characterized in that kind of cock and plants hen, and the quantity ratio of the two is 1: 6-10.
3. fertilization method according to claim 1, the semen collection time that it is characterized in that kind of cock is 2:30-3:30 in afternoon, the semen deposition time of planting hen is 2:00-8:00 in afternoon.
4. fertilization method according to claim 3, the semen deposition time that it is characterized in that kind of hen is after 4:00 in afternoon.
5. fertilization method according to claim 1, the volume of semen that it is characterized in that every kind cock is 0.3-0.6mL; The semen deposition degree of depth of planting hen is that inseminating syringe inserts 1.5cm in vagina, every kind hen semen deposition 0.025-0.03ml.
6. fertilization method according to claim 1, the system of selection that it is characterized in that kind of cock is multistage screening, until select meet semen collection demand cock as kind of a cock.
7. fertilization method according to claim 6, is characterized in that the system of selection of kind of cock comprises following four steps: for the first time: in 1 age in days cock, eliminate small and weak cock; For the second time: the cock that reservation physically well develops in 60 age in days left and right cocks, cockscomb is scarlet; For the third time: in age in 19-21 week, in cock, retain secondary sexual character good, physically fit, have the cock of sexual reflex; The 4th time: in the cock about 24 week age, eliminate aspermia or the poor cock of semen quality, the cock retaining through above-mentioned four screening processes screening is as kind of a cock.
8. fertilization method according to claim 7, is characterized in that also comprising after extracted race cock the step that it is trained, and the kind cock between 180-200 ages in days is trained continuously until semen deposition.
9. fertilization method according to claim 1, is characterized in that kind of a cock adopts single cage to raise, 16-24 ℃ of hen house temperature, and humidity 55%, keeps ventilated, and light application time is constant is 15-16 hours/days.
10. fertilization method according to claim 1, is characterized in that the semen deposition of described kind of hen specifically comprises two steps: 1) turn over anus: the cloaca of kind of hen is outwards routed up, exposed the rectum mouth on right side and the introitus in left side; 2) semen deposition: have the inseminating syringe of seminal fluid to insert vagina by inhaling, slowly inject seminal fluid.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310533125.6A CN103563848A (en) | 2013-11-01 | 2013-11-01 | Fertilization method for Tibetan chicken |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310533125.6A CN103563848A (en) | 2013-11-01 | 2013-11-01 | Fertilization method for Tibetan chicken |
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| CN103563848A true CN103563848A (en) | 2014-02-12 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885993A (en) * | 2015-05-04 | 2015-09-09 | 霍邱县科瑞达禽业有限公司 | Artificial insemination method for breeding chicken |
| CN105769377A (en) * | 2016-03-21 | 2016-07-20 | 广东温氏食品集团股份有限公司 | Novel semen deposition method for breeding hens |
| CN105941303A (en) * | 2016-04-28 | 2016-09-21 | 广西凤翔集团畜禽食品有限公司 | Method for improving fertilization rate of Guangxi local breeding chicken |
| CN107047455A (en) * | 2017-03-17 | 2017-08-18 | 枞阳县恒祥生态农业有限公司 | A kind of method for improving free range chicken fertility rate of hatching egg |
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| CN102369907A (en) * | 2011-09-17 | 2012-03-14 | 兰州宏庆科技有限公司 | Method for breeding special wild chicken through artificial fertilization |
| CN102986595A (en) * | 2012-12-11 | 2013-03-27 | 凭祥市斗鸡协会 | Methods for breeding and artificially fertilizing game chickens |
| EP2639298A1 (en) * | 2010-11-09 | 2013-09-18 | Hiroshima University | Diluent for frozen human sperm, dilution method for frozen human sperm, and adjustment method for human semen for in vitro fertilization or semen for artificial insemination |
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| EP2639298A1 (en) * | 2010-11-09 | 2013-09-18 | Hiroshima University | Diluent for frozen human sperm, dilution method for frozen human sperm, and adjustment method for human semen for in vitro fertilization or semen for artificial insemination |
| CN102369907A (en) * | 2011-09-17 | 2012-03-14 | 兰州宏庆科技有限公司 | Method for breeding special wild chicken through artificial fertilization |
| CN102986595A (en) * | 2012-12-11 | 2013-03-27 | 凭祥市斗鸡协会 | Methods for breeding and artificially fertilizing game chickens |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885993A (en) * | 2015-05-04 | 2015-09-09 | 霍邱县科瑞达禽业有限公司 | Artificial insemination method for breeding chicken |
| CN105769377A (en) * | 2016-03-21 | 2016-07-20 | 广东温氏食品集团股份有限公司 | Novel semen deposition method for breeding hens |
| CN105941303A (en) * | 2016-04-28 | 2016-09-21 | 广西凤翔集团畜禽食品有限公司 | Method for improving fertilization rate of Guangxi local breeding chicken |
| CN107047455A (en) * | 2017-03-17 | 2017-08-18 | 枞阳县恒祥生态农业有限公司 | A kind of method for improving free range chicken fertility rate of hatching egg |
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Application publication date: 20140212 |