CN101889558A - Method for inducing Verasper moseri sperm maturation and enhancing activity by using slow releasing hormone - Google Patents
Method for inducing Verasper moseri sperm maturation and enhancing activity by using slow releasing hormone Download PDFInfo
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- CN101889558A CN101889558A CN2010102309261A CN201010230926A CN101889558A CN 101889558 A CN101889558 A CN 101889558A CN 2010102309261 A CN2010102309261 A CN 2010102309261A CN 201010230926 A CN201010230926 A CN 201010230926A CN 101889558 A CN101889558 A CN 101889558A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a method for inducing Verasper moseri sperm maturation and enhancing activity by using slow releasing hormone. Sexually mature parent fish is selected, and salmon gonadotropin releasing hormone (sGnRH) is implanted into the back muscle (35 mu g/kg) to promote the sperm to finally become mature and enhance the sperm activity to more than 85-90%, thereby increasing the fertility rate by 4-7 times. The method has the advantages of low reproductive menace for parent fish, high survival rate and sex gland maturation rate of parent fish, and favorable spawning effect, and can obtain massive high quality oosperms. The method is a complete control technique. When the sperm quality starts to degrade, implantation is carried out on the parent fish for the second time, and the repeating effect is good. The method can ensure the large-scale artificial propagation of fries to be smooth.
Description
Technical field:
The invention belongs to verasper moseri genital regulating technology, is a kind of method of utilizing exogenous hormone to promote the final maturation of the male parent population sperm of verasper moseri and promoting sperm viability.
Background technology:
Verasper moseri (Verasper moseri) is the large-scale flounder class of dwelling at the bottom of a kind of cold water, mainly be distributed in to the north of the Ibaraki, Japan the pacific rim and if the sea of Japan bank to the north of the narrow gulf, China's Huanghai Sea and the Bohai Sea also had distribution in the past, did not see fishery harvesting now for many years.The verasper moseri nature is lived in nearshore waters or the bay in the marine site more, and the life depth of water is generally 10-30m, 1-20 ℃ of existence water temperature, 13-20 ℃ of suitable growth water temperature, suitable growth salinity 25-33 is under suitable cultivating condition, through 1 year form, body weight can reach more than the 900 gram/tails, fast growth, the survival rate height, bait conversion ratio height, aquaculture cost is low, and the disease incidence is low, be a kind of good cultured fishes, can extensively culture in the northern area of China.2007, the verasper moseri artificial breeding technology obtained to break through, for the industrialization development that this famous-brand and high-quality fingerling is cultured is had laid a good foundation.
Propagate artificially with warm light control condition under; the male parent population of verasper moseri usually can show that the seminal fluid viscosity is too high, sperm motility is low (30%-40%), the short symptoms such as (1-6min) of sperm life; have a strong impact on obtaining of ovum fertilization rate and batch fertilized egg, hindered the development of artificial breeding technology.In conjunction with sperm structure observation result of study, its reason may be that the hypophysis gonadotropin (GtH) that environmental abnormality causes discharges not enough, thereby blood plasma neutral steroids hormonal readiness is lower, and the part sperm fails to reach final maturation (Mylonas et al., 1997).Previous studies show that, in having the bony fish milter of microphyll type spermary, seminal fluid needs to dilute by the regulation and control of endogenous sex hormone (GTH), be discharged in the vas deferens and finally excrete, if will cause semen quality to reduce (Billard et al., 1982) because of environment-stress causes sex hormone to discharge deficiency.Therefore,, utilize the activity of exogenous hormone regulation and control hypophysis, promote gonadotropin release and blood plasma sex steroid hormone to raise and to improve ratio and the vigor that sperm reaches final maturation greatly from the internal secretion angle.At present, induce for the slow-release hormone of parent population, what adopt both at home and abroad all is muscle heeling-in method and injection, and the report of other abductive approach is not arranged as yet.
Summary of the invention:
Purpose of the present invention: a kind of method of utilizing exogenous hormone to promote the final maturation of the male parent population sperm of verasper moseri and promoting sperm viability is provided, this method be to adopt breeding period the milter of seminal fluid, in muscle of back heeling-in salmon gonadotropin-releasing hormone (GRH) (sGnRH), promote sperm quality, improve fertilization rate, obtain fertilized egg in batches.
For realizing above purpose, the technical scheme that the present invention takes is:
Select sexually matured male veraspei moseri, it is carried out inducing of exogenous hormone, promote the final maturation of sperm and promote sperm viability.
Described milter: under artificial temperature light regulation measure, the ripe male parent population that can extrude the milky seminal fluid.
Described exogenous hormone is: salmon gonadotropin-releasing hormone (GRH) (sGnRH).
Described compound salmon gonadotropin-releasing hormone (GRH) sGnRH heeling-in dosage is 35 μ g/kg, and its mode is the muscle of back heeling-in.
Its mode of described heeling-in is the muscle of back heeling-in, and the heeling-in back is near 2cm place, head side line top.Repeated once every 10 days, but every tail milter heeling-in 2 times.
The present invention has the following advantages:
1, utilization of the present invention be in normal breeding period sexually matured adopt the male parent population of milky seminal fluid, adopt hormone induction, can improve the ratio that sperm reaches final maturation greatly, significantly promote sperm viability, improve fertilization rate, obtain high quality fertilized egg of scophthatmus in batches, guarantee carrying out smoothly of artificial propagation.
2, the present invention adopts muscle of back heeling-in method, and is little to parent population reproduction stimulation, is convenient to the production practices operation.
3, the present invention adopts compound salmon gonadotropin-releasing hormone (GRH) (35 μ g/kg) to induce parent population, exogenous hormone can make blood plasma neutral steroids hormone keep in a long time than high expression level, the seminal fluid viscosity obviously descends behind the 96h, sperm motility, Fast Activities rate and sperm fertilization greatly improve, and be respond well.The hormone effect time is about 72-96h, and the effect of inducing can be kept 4-6 days, can carry out the secondary heeling-in in the hormone heeling-in first time after 10 days, and effect can repeat.
4, the present invention provides technical guarantee for verasper moseri seed propagation in scale, and is significant for the industrialized development of culturing.
Embodiment:
Embodiment:
At present, not relevant for the report of growth annual period of verasper moseri milter spermary, sperm cell characteristics and sperm quality, along with the rise of verasper moseri aquaculture, seed produces the dealer the stable technology requirement that obtains of fertilized egg is seemed more and more urgent as yet.
This research is to produce Co., Ltd at Qingdao loyalty seawater and carry out in March, 2009-April, and specific implementation process is as follows:
1). parent population is selected: select artificial temperature light regulation and control down the row of being in can adopt the essence phase verasper moseri milter 8 tails (the total length 25-33cm of milky seminal fluid, body weight 540-680g), parent population with MS222 (150mg/L) anesthesia back manual compression adopt seminal fluid, microscopy is found seminal fluid viscosity height, sperm motility all is lower than 45%, sperm Fast Activities rate is lower than 30%, sperm life 1-6min.
2). the hormone heeling-in: parent population is divided into two groups, every group 4 tail, heeling-in sGnRH.Use special-purpose heeling-in utensil, according to the dosage of 35 μ g/kg, about 2cm place muscle heeling-in above close head side line.
3). sampling detects and the fertilization check: parent population places in independent cultivation the pond in and cultivates behind the heeling-in hormone, and breeding condition is the parent population of heeling-in slow-release hormone not together.24h, 48h, 72h, 96h, 192h difference collecting semen after heeling-in, and at 0h, 48h, 96h and 192h gather the 1.0ml blood sample respectively.Observation seminal fluid viscosity is existed side by side and is engraved in inspection record sperm concentration under the mirror, sperm motility, Fast Activities rate and sperm life.Blood sample is collected serum, the content of measured by radioimmunoassay sex steroid hormone testosterone (T) with the centrifugal back of 12000rpm.Behind the different time collecting semen, utilize the experiment of being fertilized of fresh high-quality ovum, the fertilization effect of check seminal fluid.
4). experimental result: after hormone entered fish body 96h, the parent population sperm quality obviously promoted.Be embodied in: the seminal fluid viscosity significantly descends, sperm motility is brought up to 90.5-98.2%, the Fast Activities rate is increased to 84.7%-92.1%, sperm life 11.06-14.3min, the plasma testosterone expression rises to 290ng/ml (96h) by 29.5ng/dl (oh), and still remains on 194.8ng/dl at 192h.Fertilization rate rises to 45.7-78.3% (96h) by 0-13.4% (before the hormone induction), and effect is comparatively remarkable.
After 10 days, after semen quality begins to descend, parent population is carried out the secondary heeling-in, repeating effect is better.
Claims (5)
1. a slow-release hormone is induced the ripe and vigor method for improving of verasper moseri sperm, and the method that it is characterized in that it is to select sexually matured male veraspei moseri, and it is carried out inducing of exogenous hormone, promotes the final maturation of sperm and promotes sperm viability.
2. slow-release hormone according to claim 1 is induced the ripe and vigor method for improving of verasper moseri sperm, it is characterized in that described male veraspei moseri, is the ripe male parent population that can extrude the milky seminal fluid under artificial temperature light regulation measure.
3. slow-release hormone according to claim 1 is induced the ripe and vigor method for improving of verasper moseri sperm, it is characterized in that described exogenous hormone, is salmon gonadotropin-releasing hormone (GRH) sGnRH.
4. slow-release hormone according to claim 3 is induced the ripe and vigor method for improving of verasper moseri sperm, it is characterized in that described salmon gonadotropin-releasing hormone (GRH) sGnRH, and heeling-in dosage is 35 μ g/kg, and its mode is the muscle of back heeling-in.
5. slow-release hormone according to claim 4 is induced the ripe and vigor method for improving of verasper moseri sperm, it is characterized in that described muscle of back heeling-in, and the position is near 2cm place, head side line top; Repeated heeling-in once every 10 days, every tail milter can be repeated twice.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010102309261A CN101889558A (en) | 2010-07-20 | 2010-07-20 | Method for inducing Verasper moseri sperm maturation and enhancing activity by using slow releasing hormone |
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| CN2010102309261A CN101889558A (en) | 2010-07-20 | 2010-07-20 | Method for inducing Verasper moseri sperm maturation and enhancing activity by using slow releasing hormone |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782939A (en) * | 2014-02-17 | 2014-05-14 | 威海圣航水产科技有限公司 | Manual induced spawning method for platichthys stellatus parental fishes |
| CN104430087A (en) * | 2014-11-27 | 2015-03-25 | 中国水产科学研究院黑龙江水产研究所 | Method for obtaining high-quality Hucho taimen sperms through screening |
| CN112175043A (en) * | 2020-10-12 | 2021-01-05 | 湖南津安生物科技有限公司 | Improved solid-phase synthesis method of salmon gonadotropin releasing hormone |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596972A (en) * | 2004-07-19 | 2005-03-23 | 中山大学 | Slow-releasing oxytocic for artificial inducting maturation and ovulation and ejaculation rockfish and use |
| CN101699997A (en) * | 2009-11-17 | 2010-05-05 | 中国水产科学研究院黄海水产研究所 | Method for inducing gonad maturity and ovulation of verasper variegates parent fish by implanting slow-release hormone |
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2010
- 2010-07-20 CN CN2010102309261A patent/CN101889558A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596972A (en) * | 2004-07-19 | 2005-03-23 | 中山大学 | Slow-releasing oxytocic for artificial inducting maturation and ovulation and ejaculation rockfish and use |
| CN101699997A (en) * | 2009-11-17 | 2010-05-05 | 中国水产科学研究院黄海水产研究所 | Method for inducing gonad maturity and ovulation of verasper variegates parent fish by implanting slow-release hormone |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782939A (en) * | 2014-02-17 | 2014-05-14 | 威海圣航水产科技有限公司 | Manual induced spawning method for platichthys stellatus parental fishes |
| CN103782939B (en) * | 2014-02-17 | 2016-01-13 | 威海圣航水产科技有限公司 | The induced spawning method of a kind of asropyle platichthys stellatus pallas parent population |
| CN104430087A (en) * | 2014-11-27 | 2015-03-25 | 中国水产科学研究院黑龙江水产研究所 | Method for obtaining high-quality Hucho taimen sperms through screening |
| CN112175043A (en) * | 2020-10-12 | 2021-01-05 | 湖南津安生物科技有限公司 | Improved solid-phase synthesis method of salmon gonadotropin releasing hormone |
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Open date: 20101124 |