CN108852553A - A kind of artificial semenpicking of goose and semen deposition method - Google Patents

A kind of artificial semenpicking of goose and semen deposition method Download PDF

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Publication number
CN108852553A
CN108852553A CN201810750152.1A CN201810750152A CN108852553A CN 108852553 A CN108852553 A CN 108852553A CN 201810750152 A CN201810750152 A CN 201810750152A CN 108852553 A CN108852553 A CN 108852553A
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gander
semen
goose
sperm
days
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CN201810750152.1A
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CN108852553B (en
Inventor
卞友庆
段修军
秦豪荣
陆艳凤
孙国波
李晓鸣
纪荣超
张干生
穆晓惠
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Jiangsu Sci-Tech Demostration Garden Of Modern Animal Husbandry
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Jiangsu Sci-Tech Demostration Garden Of Modern Animal Husbandry
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/02Breeding vertebrates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/021Apparatus for collecting seminal fluids; Artificial vaginas
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/027Devices for injecting semen into animals, e.g. syringes, guns, probes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/027Devices for injecting semen into animals, e.g. syringes, guns, probes
    • A61D19/028Supporting means thereof, e.g. saddles or belts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Reproductive Health (AREA)
  • Public Health (AREA)
  • Animal Husbandry (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Surgical Instruments (AREA)
  • Media Introduction/Drainage Providing Device (AREA)

Abstract

The invention belongs to animal feeding technical fields, are the artificial semenpicking and semen deposition method of a kind of goose.By carrying out artificial semenpicking to gander, the method for carrying out artificial insemination to female goose postpones the superseded time of gander, improves the reproductive performance of female goose, improve the utilization periods of female goose to reduce the number of animals raised of gander.To reduce the feeding cost of goose, the economic benefit for supporting goose is improved.

Description

A kind of artificial semenpicking of goose and semen deposition method
Technical field
The invention belongs to animal feeding technical fields, and in particular to a kind of method of goose artificial semenpicking and semen deposition.
Background technique
On the one hand, during the feeding process, the feeding cost of gander is higher for existing poultry, by artificial semenpicking and semen deposition, makes Poultry supports public fowl less during the feeding process, and reduces the death rate of embryo in hatching process.On the other hand, due to goose body Type is larger, low reproduction rate and broodiness is extremely strong, traditional poultry semen collection and Insemination procedures are not able to satisfy the insemination demand of goose, need Existing artificial semenpicking and semen deposition method are improved.
Summary of the invention
The purpose of the present invention is to provide a kind of artificial semenpicking of goose and semen deposition method, ancestor aims to solve the problem that raising gander cost Height, low reproduction rate, the high problem of natural mating rate of embryonic death in hatching process.
The present invention provides a kind of artificial semenpickings of goose and semen deposition method, by the gander sperm manually acquired after screening It is immediately placed in thermosistor using 9 times of diluted, and by the sperm after dilution, gradually decreases thermosistor temperature by room temperature To 5-7 DEG C, and save room temperature is gradually heated to after 8-10min again after take out artificial insemination, the dilution carried out to female goose According to mass ratio to be 14 by potassium citrate, sodium glutamate, disodium hydrogen phosphate, sodium dihydrogen phosphate, glucose and distilled water:142: 97:23:75:The mixed liquor of 10000 compositions.
Preferably, the rate of temperature fall of thermosistor is 0.5-1 DEG C/min.
Preferably, the heating rate of thermosistor is 1-1.5 DEG C/min.
Preferably, the method for artificial insemination is that the gander of artificial semenpicking selects the stronger progress single cage raising of sexual desire, isolation 5 ~7 days are to start semen collection training, are first cut the feather around gander cloaca completely before semen collection training, semen collection cage will be used and be blocked The both legs for trying feelings female goose are fixed with cotton strap and are put into gander cage, when gander catches in its mouth mother with examination feelings female goose one by device Tail feathers is waved in gooseneck boom portion again and again, while when penis base portion is exposed to outside anus, holds and collects smart cup and lean on cloaca into gander, squeezes At gander hip joint penis is routed up, by seminal fluid collecting to collecting in smart cup, it is preferred that semen collection training continues 5~7 days, 5~ Semen deposition work is carried out after 7 days.
Preferably, use technology of artificial insemination male and female ratio for 1:15~20;And gander one week semen collection 5 days, it can connect Continuous semen collection 5 days, rest 2 days or semen collection 2 days such circulations of taking a day off.
Preferably, the screening includes the visual examination and semen volume inspection of sperm, the visual examination of the sperm Whether the color to check sperm is normal, and it is opaque liquid that normal free of contamination sperm, which is creamy white, and is mixed into blood and is in Red, faecal contamination are yellow;The semen volume inspection is to be sucked sperm using the isocratic measuring device of liquid-transfering gun, measurement one Secondary ejaculation amount, ejaculation amount are changed greatly with age, season, individual difference and semen collection skilled operation degree, gander averagely ejaculation amount It is 0.2~2 milliliter, selects ejaculation amount is more, stablizes normal gander to use.
Preferably, the semen deposition method is by female goose by being in that natural mating is squatted shape, and is pressing against female goose on semen deposition platform Back combine semen deposition platform with the abdomen of female goose, squeeze the lower edge of cloaca, push nephrodinic accent aside, open cloaca outer Visual introitus is turned to, and the alignment vagina insertion of inseminating syringe catheter tip is subjected to semen deposition.
Preferably, the female goose is within laying period.
The present invention is optimized by semen collection to goose and semen deposition method, and it is at high cost to solve raising gander, low reproduction rate, The high problem of natural mating rate of embryonic death in hatching process.For dilution in the prior art to the place of the current sperm of goose Scarce capacity is managed, the present invention screens the ingredient of semen diluent according to the physilogical characteristics of goose, finds by potassium citrate, paddy Propylhomoserin sodium, disodium hydrogen phosphate, sodium dihydrogen phosphate, glucose and distilled water are suitable for goose according to the mixed liquor that special ratios form Sperm feature, on this basis, after inventor also found extension rate and the dilution of the dilution collaboration sperm of aforementioned proportion Isothermal holding operation can be such that the sperm activity of goose is promoted within the section time, be conducive to subsequent semen deposition process, to make The fertility rate and hatchability for obtaining goose has obtained improvement by a relatively large margin.
Specific embodiment
Embodiment 1
One, materials and methods
1, test period and mode
1.1 male and female geese are split up into 5~7 days.
1.2 artificial semenpickings and semen deposition and natural mating two ways.
2, the method for artificial semenpicking
The gander of artificial semenpicking selects the stronger progress single cage raising of sexual desire, and isolation 5~7 days start semen collection, will be public before semen collection Feather around goose cloaca is cut completely, and semen collection cage will use radical occlusion device, and gander is allowed to can't see semen collection people in semen collection.Semen collection When with examination feelings female goose one, the both legs for trying feelings female goose are fixed with cotton strap and are put into gander cage, when gander catches in its mouth female goose Tail feathers is waved on head again and again, while when penis base portion starts to expose to outside anus, and semen collection person holds the smart cup of collection with the right hand and leans on into gander Cloaca, left hand gently squeezes at gander hip joint, and the right hand, which is taken, collects smart cup, make to collect smart rim of a cup and resist female goose cloaca, to It is upper firmly gander penis to be helped to rout up, it will be in semen collection to the smart cup of collection.
3, the dilution process of sperm
Using special dilution(Contain potassium citrate 0.14g, sodium glutamate 1.42g, phosphoric acid hydrogen two in every 100 milliliters of distilled water Sodium 0.97g, sodium dihydrogen phosphate 0.23g, glucose 0.75g)For the dilution of goose sperm, by 9 times of initial semen dilution.And it will be dilute Sperm after releasing is immediately placed in thermosistor, setting program make thermosistor temperature by room temperature according to 0.5 DEG C/min speed by 5 DEG C are gradually reduced to, and room temperature is gradually heated to the speed of 1 DEG C/min again after saving 10min, rear take out carries out manually female goose Semen deposition.
4, the method for goose female goose semen deposition
Female goose is pressed on semen deposition platform, semen deposition person is clipped in female goose between two legs, so that female goose is squatted shape in natural mating, semen deposition person Semen deposition platform is combined with the abdomen of female goose with the back that left hand and right hand is pressing against female goose, is gently squeezed the lower edge of cloaca, is used index finger It gently pushes nephrodinic accent aside, opens cloaca, at this moment, we can see two apertures, and one, the right aperture is defecation urine Rectum mouth, one, left side aperture is exactly introitus, vacates the right hand for inseminating syringe catheter tip and is directed at insertion 2~3cm of vagina progress Semen deposition.
Comparative example 1
It is inseminated using natural mating mode
Comparative example 2
All processes keep almost the same with embodiment 1, the difference is that diluted multiple is 8 times.
Comparative example 3
All processes keep almost the same with embodiment 1, the difference is that diluted multiple is 10 times.
Comparative example 4
All processes keep almost the same with embodiment 1, the difference is that will not be in 20 DEG C of incubators of sperm after dilution 5min, but directly semen deposition operation.
Except comparative example 1 uses 20(♂), 100(♀)External as trial flock, embodiment 1 and comparative example 2-4 are all made of 10(♂), 100(♀)As test group, by 30 days group experiments, the Contrast on effect of each group analyzed result such as table 1 It is shown.
Influence of the different semen deposition modes of table 1 to goose reproductive performance
As shown in Table 1, using the method for the present embodiment, three item data of fertility rate of hatching egg, hatching of breeding eggs rate and hatchability of fertile eggs Be significantly better than that comparative example, it can be seen that, the present invention is based on semen collection and insemination technique main points, establish a kind of goose artificial semenpicking with The method of semen deposition effectively improves goose hatching of breeding eggs rate, improves the utilization efficiency of kind of gander, reduces feeding cost.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.

Claims (8)

1. a kind of artificial semenpicking of goose and semen deposition method, it is characterised in that by the gander sperm manually acquired using dilute after screening It releases liquid and dilutes 9 times, and the sperm after dilution is immediately placed in thermosistor, thermosistor temperature is made to be gradually decrease to 5-7 by room temperature DEG C, and save room temperature is gradually heated to after 8-10min again after take out artificial insemination carried out to female goose, the dilution is by lemon Lemon acid potassium, sodium glutamate, disodium hydrogen phosphate, sodium dihydrogen phosphate, glucose and distilled water are 14 according to mass ratio:142:97:23: 75:The mixed liquor of 10000 compositions.
2. according to the method described in claim 1, it is characterized in that the rate of temperature fall of the thermosistor is 0.5-1 DEG C/min.
3. according to the method described in claim 1, it is characterized in that the heating rate of the thermosistor is 1-1.5 DEG C/min.
4. according to the method described in claim 1, it is characterized in that the method for artificial insemination is optional for the gander of artificial semenpicking It is intended to stronger progress single cage raising, isolation 5~7 days start semen collection training, first will be around gander cloaca before semen collection training Feather is cut completely, and semen collection cage will use radical occlusion device, with examination feelings female goose one, the both legs for trying feelings female goose is fixed with cotton strap and are put Enter in gander cage, when gander catches in its mouth female goose head, wave tail feathers again and again, while when penis base portion is exposed to outside anus, holding Collect smart cup and lean on cloaca into gander, squeezes and at gander hip joint penis is routed up, seminal fluid collecting is arrived into collection essence cup, it is excellent Choosing, semen collection training carries out semen deposition work after continuing 5~7 days, 5~7 days.
5. according to the method described in claim 1, it is characterized in that using technology of artificial insemination male and female ratio for 1:15~20;And Gander one week semen collection 5 days, can be semen collection 5 days continuous, rest 2 days or semen collection 2 days such circulations of taking a day off.
6. according to the method described in claim 1, it is characterized in that the screening includes the visual examination and semen volume of sperm It checks, the visual examination of the sperm is to check whether the color of sperm is normal, and normal free of contamination sperm is creamy white, and is Opaque liquid, and be mixed into blood and take on a red color, faecal contamination is yellow;The semen volume inspection is using liquid-transfering gun etc. Device is measured, sperm is sucked, measures primary ejaculation amount, ejaculation amount is with age, season, individual difference and semen collection skilled operation degree It changes greatly, gander averagely ejaculation amount is 0.2~2 milliliter.
7. according to the method described in claim 1, it is characterized in that the semen deposition method is to be in by semen deposition platform by female goose Natural mating is squatted shape, and the back for pressing against female goose combines semen deposition platform with the abdomen of female goose, squeezes the lower edge of cloaca, is dialled Nephrodinic accent is opened, makes cloaca opening is outer to turn to visual introitus, and the alignment vagina insertion progress of inseminating syringe catheter tip is defeated Essence.
8. according to the method described in claim 1, it is characterized in that the female goose is within laying period.
CN201810750152.1A 2018-07-10 2018-07-10 Artificial semen collection and insemination method for geese Active CN108852553B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112630457A (en) * 2020-12-25 2021-04-09 河南农业大学 Method for screening goose wings based on blood biochemical indexes and application thereof

Citations (4)

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Publication number Priority date Publication date Assignee Title
CN86101836A (en) * 1986-03-22 1987-01-24 中国农业科学院畜牧研究所 Solution for dilution of cock semen
CN103271779A (en) * 2013-05-13 2013-09-04 沅江市湘丰鹅业发展有限公司 Artificial fertilization method for breeding geese
CN103598142A (en) * 2013-11-16 2014-02-26 罗平县智龙养鹅专业合作社 Diandong goose and cultivation method thereof
CN105875503A (en) * 2016-04-29 2016-08-24 西南大学荣昌校区 Treatment method for increasing fertilization rate of white goose

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
CN86101836A (en) * 1986-03-22 1987-01-24 中国农业科学院畜牧研究所 Solution for dilution of cock semen
CN103271779A (en) * 2013-05-13 2013-09-04 沅江市湘丰鹅业发展有限公司 Artificial fertilization method for breeding geese
CN103598142A (en) * 2013-11-16 2014-02-26 罗平县智龙养鹅专业合作社 Diandong goose and cultivation method thereof
CN105875503A (en) * 2016-04-29 2016-08-24 西南大学荣昌校区 Treatment method for increasing fertilization rate of white goose

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112630457A (en) * 2020-12-25 2021-04-09 河南农业大学 Method for screening goose wings based on blood biochemical indexes and application thereof
CN112630457B (en) * 2020-12-25 2022-12-27 河南农业大学 Method for screening goose wings based on blood biochemical indexes and application thereof

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