CN103070162B - Muscovy duck semen diluent - Google Patents
Muscovy duck semen diluent Download PDFInfo
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- CN103070162B CN103070162B CN201310035006.8A CN201310035006A CN103070162B CN 103070162 B CN103070162 B CN 103070162B CN 201310035006 A CN201310035006 A CN 201310035006A CN 103070162 B CN103070162 B CN 103070162B
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Abstract
The invention relates to a muscovy duck semen diluent which comprises the following raw materials: 1.6-2.4g/100mL of sodium glutamate, 0.8-1.2g/100mL of glucose, 0.3-0.6g/100mL of monopotassium phosphate, 0.1-0.3g/100mL of magnesium chloride, 0.6-1.0g/100mL of sodium bicarbonate, 60000-80000IU/100mL of streptomycin, and the balance of distilled water. A preparation method of the muscovy duck semen diluent comprises the steps of adding sodium glutamate, glucose, monopotassium phosphate, magnesium chloride, sodium bicarbonate and streptomycin at a raw material ratio, then adding the distilled water to 100mL, and regulating a pH (Potential of Hydrogen) value to be 6.5-7.2. Tests indicate that muscovy duck semen is diluted by the muscovy duck semen diluent at a ratio of 1:1 and 1:2, the average fertilization rate of hatching eggs is higher than the fertilization rate when the raw semen is diluted by normal saline at a ratio of 1:1 and 1:2, and the muscovy duck semen diluent can be popularized and used in the production process.
Description
Technical field
The invention belongs to animal reproduction technical field, relate in particular to a kind of kind duck semen diluent.
Background technology
Half kind of duck is the filial generation of kind duck and family duck, and because kind duck is the duck kind not belonging to together with a duck, the fertilization rate of natural mating is lower, and average fertilization rate is only 30% ~ 45%.Technology of artificial insemination is more and more extensive in poultry Breeding Application, and the height of its level directly affects the economic benefit of plant.At present, half kind of duck produces the technology of artificial insemination that adopt more, and fertilization rate is generally between 60% ~ 80%.
The quality of seminal fluid has determined test-tube success or failure, most important to the fertilization rate of kind of fowl.On business fowl lacks sexual accessory gland, nutrition content in seminal fluid is little, the deposit of the endogenous energy of sperm itself is also very limited, in the very short time, semen quality will reduce greatly in vitro, and this has just limited the sperm time-to-live in vitro to a great extent, therefore carries out poultry artificial insemination, directly utilize seminal fluid, the fresh semen that General Requirements gathers is finished in 20 ~ 30 min, and this has brought certain difficulty just to test-tube work, has limited the utilization ratio of good breeding male fowl.In order to improve the availability of breeding male fowl and the fertilization rate of kind egg, seminal fluid is diluted very common.Sperm dilution not only can extend the sperm time-to-live in vitro, strengthens the vigor of sperm, and can increase sperm volume.Simultaneously can be to makeup energy material and nutrient component in seminal fluid by dilution.Good semen diluent should possess the desirable living environment of sperm, and neutralization or eliminating are to the dysgenic factor of sperm.In addition, semen diluent also need to have draw materials easily, configuration is simple, cost is low, be convenient to the features such as popularization.In recent ten years, the research of kind duck semen diluent increases gradually, but not yet has so far a comparatively desirable dilution formula.
Summary of the invention
The object of the present invention is to provide a kind of kind duck semen diluent, for diluting the seminal fluid of kind duck, be intended to improve the viability of sperm, improve fertilization rate.Technical scheme of the present invention is as follows:
The content of the each composition of dilution of the present invention is: sodium glutamate 1.6 ~ 2.4 g/100 mL, glucose 0.8 ~ 1.2 g/100 mL, potassium dihydrogen phosphate 0.3 ~ 0.6 g/100 mL, magnesium chloride 0.1 ~ 0.3 g/100 mL,
Sodium bicarbonate 0.6 ~ 1.0 g/100 mL, streptomycin 60,000 ~ 80,000 IU/100 mL;
The preparation method of above-mentioned dilution is: content adds above-mentioned composition in accordance with regulations, then adding distil water to 100 mL, and pH value is adjusted between 6.5 ~ 7.2.
Preferred version of the present invention is: sodium glutamate 2.0 g/100 mL, glucose 1.0 g/100 mL, potassium dihydrogen phosphate 0.45 g/100 mL, magnesium chloride 0.2 g/100 mL, sodium bicarbonate 0.8 g/100 mL, streptomycin 70,000 IU/100 mL, adding distil water to 100 mL, pH value is 6.8.
Dilution its theoretical foundation of filling a prescription that the present invention proposes is that sodium glutamate has good protective effect to sperm, and sperm viability is had to desirable influence; Glucose can provide the energy of sperm in vitro existence; Potassium dihydrogen phosphate is buffer substance, makes to dilute seminal fluid and can keep certain acid-base value and buffer environment; Magnesium ion in magnesium chloride has the motion of protection sperm and extends sperm life feature; Mucus in sodium bicarbonate solubilized seminal fluid, can fully mix seminal fluid, and maintains the osmotic equilibrium of environment in dilution environment and sperm; Streptomycin has bactericidal action, can reduce the infection of female bird genital tract in semen deposition process.
Dilution its fertilization rate experimental basis of filling a prescription that the present invention proposes is, utilizes kind duck to produce half kind of duck with changing in vain duck hybridization, and test is chosen 2000 and is in that raising in cages of egg-laying peak is white changes duck, is divided at random 5 groups, and every group has 2 repetitions.Semen deposition time unification is selected 8:00 ~ 11:00 in the morning, collects altogether the kind egg of 4 days.Result shows that the average fertilization rate of the former seminal fluid of I group is 76.05 ± 0.78%; II group physiological saline is pressed 1:1 dilution proportion, and average fertilization rate is 75.62 ± 0.53%; III group physiological saline is pressed 1:2 dilution proportion, and average fertilization rate is 74.73 ± 1.29%; IV group is used dilution of the present invention to press 1:1 dilution proportion, and average fertilization rate reached is to 77.77 ± 1.08%; V group is used dilution of the present invention to press 1:2 dilution proportion, and average fertilization rate reached is to 76.44 ± 0.62%; Show to utilize this dilution to kind duck seminal fluid according to the dilution proportion of 1:1,1:2, plant the average fertilization rate of egg all higher than the fertilization rate of former seminal fluid and physiological saline 1:1,1:2 dilution proportion, can be applicable aborning.
Remarkable advantage of the present invention: one provided by the invention kind duck semen diluent, the results showed the present invention to kind duck seminal fluid according to the dilution proportion of 1:1,1:2, the average fertilization rate of kind of egg all, higher than the fertilization rate of former seminal fluid and physiological saline 1:1,1:2 dilution proportion, can be applicable aborning.Of the present invention kind of duck semen diluent is applicable to a kind duck fresh semen to dilute laggard row artificial insemination, improves the sperm time-to-live in vitro, keeps sperm viability.Preparation method of the present invention is simple, draws materials conveniently, with low cost, can large scale application.
Embodiment
(1) diluent ingredient of the present invention: mainly comprise sodium glutamate, glucose, potassium dihydrogen phosphate, magnesium chloride, sodium bicarbonate, streptomycin and distilled water.
(2) diluent preparing of the present invention: will note following item when preparation.1, all apparatus that configuration dilution uses, thoroughly washes clean, after sterilization, can be used; 2, dilution need keep fresh, preferably now with the current, as condition does not allow, can in refrigerator, deposit 1 week; 3, distilled water pH used should be neutral; 4, prepared and diluted liquid medicine used, composition is pure, weighs and wants accurately, dissolves and wants fully, to carry out disinfection after filtration after sealing; 5, the interpolation of streptomycin, must, in the time that dilution is cooled to room temperature, accurately add by consumption.
(3) dilution process of the present invention: 1, after semen collection, seminal fluid should dilute immediately, seminal fluid longer its motility rate standing time is lower, and dead number of sperm is more; 2, dilution and the temperature of seminal fluid must be adjusted unanimously, avoid excessive temperature differentials, and sperm is caused to damage; 3, be 1:1 or 1:2 with the dilution ratio of seminal fluid, in actual production, the ratios that adopt 1:1 are diluted more; 4, while dilution, dilution is slowly added along the smart wall of cup of collection, prevent dilution to pour into rapidly in seminal fluid, cause local dilution to hit; 5, after dilution, will collect essence cup and rotate gently, seminal fluid is mixed with dilution, must guard against concuss.
Enumerate specific embodiment below, further describe the present invention, but the present invention is not limited to this.
embodiment 1:one of the present invention kind duck dilution is raw material composition according to the following ratio:
Sodium glutamate 1.6g/100 mL, glucose 0.8 g/100 mL, potassium dihydrogen phosphate 0.3 g/100 mL, magnesium chloride 0.1g/100 mL, sodium bicarbonate 0.6 g/100 mL, streptomycin 60,000 IU/100 mL.
Its preparation method is: add the raw material of above-mentioned proportioning components, then adding distil water to 100 mL, pH value is adjusted to 6.5.
embodiment 2:one of the present invention kind duck dilution is raw material composition according to the following ratio:
Sodium glutamate 2.4 g/100 mL, glucose 1.2 g/100 mL, potassium dihydrogen phosphate 0.6 g/100 mL, magnesium chloride 0.3 g/100 mL, sodium bicarbonate 1.0 g/100 mL, streptomycin 80,000 IU/100 mL.
Its preparation method is: add the raw material of above-mentioned proportioning components, then adding distil water to 100 mL, pH value is adjusted to 7.2.
embodiment 3:
One of the present invention kind duck dilution is raw material composition according to the following ratio:
Sodium glutamate 2.0 g/100 mL, glucose 1.0 g/100 mL, potassium dihydrogen phosphate 0.45 g/100 mL, magnesium chloride 0.2 g/100 mL, sodium bicarbonate 0.8 g/100 mL, streptomycin 70,000 IU/100 mL.
Its preparation method is: add the raw material of above-mentioned proportioning components, then adding distil water to 100 mL, pH value is adjusted to 6.8.
Claims (3)
1. one kind of kind of duck semen diluent, it is characterized in that: described dilution is raw material composition according to the following ratio: sodium glutamate 2.0 g/100 mL, glucose 1.0 g/100 mL, potassium dihydrogen phosphate 0.45 g/100 mL, magnesium chloride 0.2 g/100 mL, sodium bicarbonate 0.8 g/100 mL, streptomycin 70,000 IU/100 mL.
2. the preparation method of a kind of kind of duck semen diluent as claimed in claim 1, it is characterized in that: described preparation method is, add sodium glutamate, glucose, potassium dihydrogen phosphate, magnesium chloride, sodium bicarbonate, the streptomycin of raw material proportioning, adding distil water to 100 mL again, tune pH value is 6.5-7.2.
3. the preparation method of a kind of kind of duck semen diluent according to claim 2, it is characterized in that: described preparation method is, add sodium glutamate 2.0 g, glucose 1.0 g, potassium dihydrogen phosphate 0.45 g, magnesium chloride 0.2 g, sodium bicarbonate 0.8 g, streptomycin 70,000 IU, then adding distil water to 100 mL, adjusting pH value is 6.8.
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| CN201310035006.8A CN103070162B (en) | 2013-01-30 | 2013-01-30 | Muscovy duck semen diluent |
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| CN201310035006.8A CN103070162B (en) | 2013-01-30 | 2013-01-30 | Muscovy duck semen diluent |
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| CN103070162B true CN103070162B (en) | 2014-10-08 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103348967B (en) * | 2013-07-15 | 2014-10-08 | 福建省农业科学院畜牧兽医研究所 | Semen diluting solution for artificial insemination of poultry and application method thereof |
| CN103609552A (en) * | 2013-12-06 | 2014-03-05 | 安徽五星食品股份有限公司 | Poultry semen diluent for maintaining sperm motility and prolonging insemination time |
| CN107913118A (en) * | 2017-12-19 | 2018-04-17 | 佛山科学技术学院 | A kind of mule duck artificial insemination method |
| CN111000658A (en) * | 2019-12-25 | 2020-04-14 | 西北农林科技大学 | Muscovy duck female duck artificial insemination method |
| CN110946132A (en) * | 2019-12-31 | 2020-04-03 | 赵章成 | Poultry semen nutrient, nutrient diluent, preparation method and application thereof |
| CN113812396B (en) * | 2021-09-28 | 2022-09-16 | 广东海洋大学 | Duck essence cryopreservation diluent |
| CN113973811A (en) * | 2021-11-26 | 2022-01-28 | 华中农业大学 | Muscovy duck semen diluent and application thereof |
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| CN86101836A (en) * | 1986-03-22 | 1987-01-24 | 中国农业科学院畜牧研究所 | Solution for dilution of cock semen |
| CN101543209A (en) * | 2009-03-31 | 2009-09-30 | 华南农业大学 | Powder for chicken sperm dilution and preservation and preparation method and application thereof |
| CN102812950A (en) * | 2012-09-07 | 2012-12-12 | 江苏省家禽科学研究所 | Chicken semen diluent as well as preparation method and application thereof |
| CN102841007A (en) * | 2012-09-05 | 2012-12-26 | 福建省农业科学院畜牧兽医研究所 | Semen diluent convenient to count duck sperms and application method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101109410B1 (en) * | 2009-04-24 | 2012-06-27 | 대한민국 | An artificial seminal plasma for sperm cryopreservation in grouper |
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Patent Citations (4)
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| CN86101836A (en) * | 1986-03-22 | 1987-01-24 | 中国农业科学院畜牧研究所 | Solution for dilution of cock semen |
| CN101543209A (en) * | 2009-03-31 | 2009-09-30 | 华南农业大学 | Powder for chicken sperm dilution and preservation and preparation method and application thereof |
| CN102841007A (en) * | 2012-09-05 | 2012-12-26 | 福建省农业科学院畜牧兽医研究所 | Semen diluent convenient to count duck sperms and application method thereof |
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