CN104396941A - Chicken sperm diluting liquid and preparation method thereof - Google Patents

Chicken sperm diluting liquid and preparation method thereof Download PDF

Info

Publication number
CN104396941A
CN104396941A CN201410621015.XA CN201410621015A CN104396941A CN 104396941 A CN104396941 A CN 104396941A CN 201410621015 A CN201410621015 A CN 201410621015A CN 104396941 A CN104396941 A CN 104396941A
Authority
CN
China
Prior art keywords
dilution
semen
cock
solution
sperm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410621015.XA
Other languages
Chinese (zh)
Other versions
CN104396941B (en
Inventor
张英芹
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Taishan Secco Agricultural Technology Co Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201410621015.XA priority Critical patent/CN104396941B/en
Publication of CN104396941A publication Critical patent/CN104396941A/en
Application granted granted Critical
Publication of CN104396941B publication Critical patent/CN104396941B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a chicken sperm diluting liquid. The sterile steamed water is taken as the solvent, and each 1000 mL of the diluting liquid comprises the following components by weight: 1.0 gram of magnesium chloride hexahydrate, 1.15 grams of potassium citrate, 9.5 grams of sodium acetate trihydrate, 12.5 grams of fructose, 15.2 grams of sodium glutamate, 3 grams of composite vitamin, 0.0024 to 0.0246 mg of cerium chloride, and the balance being solvent. The pH value of the chicken sperm diluting liquid is 6.8 to 7.0, and the penetration pressure is 375-380 mmol/L/kg. Compared to the conventional chicken sperm diluting liquids, the provided chicken sperm diluting liquid can improve the sperm activity, effectively prolong the sperm survival time, prominently increase the utilizing rate of breeding cocks, and reduce the phenomenon of stress reactions and oviduct inflammation of hens during the artificial insemination process.

Description

A kind of solution for dilution of cock semen and preparation method thereof
Technical field
The present invention relates to a kind of dilution, especially relate to a kind of solution for dilution of cock semen and preparation method thereof.
Background technology
Along with the universal of cage poultry and development, the artificial insemination of chicken is able to extensive popularization.The technology of artificial insemination difficulty of chicken is little, easily grasps, and equipment is simple, small investment.The artificial insemination of chicken can reduce the number of animals raised of stock cock, and during natural mating, the male and female ratio of general chicken is 10-15 only left and right, and when adopting artificial insemination, and as by inseminating weekly once, then 1 cock can join 100 even more hens.For ensureing the high fertilization rate of kind of egg in production practices, generally adopt semen deposition in 3-5 days once more.Calculate by this, during artificial insemination, the male and female ratio of chicken mostly is about 1:50.Than adopting natural mating method, can reduce by the cock number of animals raised of 80%, reducing kind of an egg cost.When adopting artificial insemination, male and female chicken is sub-cage rearing.Sub-cage rearing, can be breeding work and provides convenience, and also provides condition for supplementing the nutrients separately to cock, is conducive to improving kind of a Chicken Semen quality.When adopting artificial insemination under normal circumstances, cock all adopts single cage to raise, thus reduces and peck bucket, reduction death rate between chicken.
The realization of above-mentioned superiority mainly depends on the design of good semen diluent, screening and use.The dilution of Chicken Semen is the diluted with particular formulation.The freezen protective of Chicken Semen is still under test.To actual production, with the fresh more convenient practicality of seminal fluid semen deposition, therefore at this, preservation of Chicken Semen and dilution process are not described.Even if it should be noted that and adopt fresh semen semen deposition, after Chicken Semen extraction cock is external, if environmental temperature is too low also can affect its fertilization rate.So, when environmental temperature is lower than 20 DEG C, preferably adopt the smart cup collection essence of insulation collection.Pour into the warm water of 32 DEG C in thermos cup, if collect smart cup without special insulation during practical operation, also with additive method to the smart cup insulation of collection, such as by teat glass collection essence, can then be placed in the vessel filling warm water.Excellent semen diluent should possess the desirable living environment of sperm, and neutralization or eliminating are to the dysgenic factor of sperm.In addition, semen diluent also should have draw materials easily, configuration is simple, cost is low, be convenient to the features such as popularization.Although the research of solution for dilution of cock semen increases gradually at present, not yet there is the dilution formula that ideal so far.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of solution for dilution of cock semen that sperm viability can be made to improve, extend Sperm survival time.
Another technical problem to be solved by this invention improves a kind of method preparing above-mentioned solution for dilution of cock semen.
Technical problem to be solved by this invention is achieved by the following technical programs:
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0024 ~ 0.0246mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Preferably, described cerium chloride is 0.0036mg.
Preferably, penicillin 400,000 IU, streptomycin 400,000 IU is also comprised.
A kind of method preparing arbitrary above-mentioned solution for dilution of cock semen, it is characterized in that, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride and/or penicillin and streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The present invention has following beneficial effect: compared with existing solution for dilution of cock semen, and solution for dilution of cock semen of the present invention has: (1) improves sperm viability; (2) effectively Sperm survival time is extended; (3) utilization rate of stock cock is significantly improved; (4) reduce Breeding hens artificial insemination process produce stress, the problem such as salpingitis tubal.
Embodiment
Below in conjunction with embodiment, the present invention will be described in detail, and embodiment is only the preferred embodiment of the present invention, is not limitation of the invention.
Embodiment 1
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0024mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
Embodiment 2
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0036mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
Embodiment 3
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0246mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
Embodiment 4
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0036mg, penicillin 400,000 IU, streptomycin 400,000 IU, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, penicillin, streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
Comparative example 1
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, penicillin 400,000 IU, streptomycin 400,000 IU, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add penicillin, streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
In 0 ~ 72 hour, observe the sperm viability of embodiment 1 ~ 4, comparative example 1, and measured motility rate and the abnormal rate of sperm every 24 hours, the results are shown in Table 1.
Table 1 different diluent Cord blood seminal fluid is on the impact of chicken sperm motility rate
As can be seen from Table 1, embodiment 1 ~ 4 is compared with comparative example 1, and after adding cerium chloride, the survival rate of sperm obviously extends, and the abnormal rate of sperm obviously reduces; When adding the cerium chloride of 0.0036mg, the survival rate of sperm is better, and abnormal rate is lower.
Solution for dilution of cock semen provided by the invention, because the vigor of sperm extends, makes twice Inseminated time interval extend, thus reduces the amount of labour, save labour cost.The utilization rate of stock cock can be provided simultaneously, reduce artificial insemination stress and the generation of salpingitis tubal, the laying rate of Breeding hens is provided.

Claims (4)

1. a solution for dilution of cock semen, it is characterized in that, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0024 ~ 0.0246mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
2. solution for dilution of cock semen according to claim 1, is characterized in that, described cerium chloride is 0.0036mg.
3. solution for dilution of cock semen according to claim 1 and 2, is characterized in that, also comprises penicillin 400,000 IU, streptomycin 400,000 IU.
4. prepare the method for the arbitrary described solution for dilution of cock semen of claims 1 to 3 for one kind, it is characterized in that, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride and/or penicillin and streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
CN201410621015.XA 2014-11-07 2014-11-07 A kind of solution for dilution of cock semen and preparation method thereof Active CN104396941B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410621015.XA CN104396941B (en) 2014-11-07 2014-11-07 A kind of solution for dilution of cock semen and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410621015.XA CN104396941B (en) 2014-11-07 2014-11-07 A kind of solution for dilution of cock semen and preparation method thereof

Publications (2)

Publication Number Publication Date
CN104396941A true CN104396941A (en) 2015-03-11
CN104396941B CN104396941B (en) 2016-07-06

Family

ID=52634669

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410621015.XA Active CN104396941B (en) 2014-11-07 2014-11-07 A kind of solution for dilution of cock semen and preparation method thereof

Country Status (1)

Country Link
CN (1) CN104396941B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111406738A (en) * 2020-05-09 2020-07-14 华南农业大学 Chicken essence diluent for artificial oviduct insemination and preparation method thereof
CN113940340A (en) * 2021-10-28 2022-01-18 广西富凤农牧集团有限公司 Chicken sperm diluent and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030217380A1 (en) * 2002-03-14 2003-11-20 Rutledge Jackie J. Composition containing gamete or embryo and animal white yolk and the use thereof
CN103609552A (en) * 2013-12-06 2014-03-05 安徽五星食品股份有限公司 Poultry semen diluent for maintaining sperm motility and prolonging insemination time
CN103704205A (en) * 2014-01-15 2014-04-09 山东大学 Bovine semen freezing dilute solution containing trace amount of rare-earth and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030217380A1 (en) * 2002-03-14 2003-11-20 Rutledge Jackie J. Composition containing gamete or embryo and animal white yolk and the use thereof
CN103609552A (en) * 2013-12-06 2014-03-05 安徽五星食品股份有限公司 Poultry semen diluent for maintaining sperm motility and prolonging insemination time
CN103704205A (en) * 2014-01-15 2014-04-09 山东大学 Bovine semen freezing dilute solution containing trace amount of rare-earth and application thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张淑娟: "鸡精液不同稀释液配方筛选的研究", 《吉林农业大学硕士学位论文》 *
采克俊等: "种公鸡精液稀释液的研究", 《安徽农业科学》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111406738A (en) * 2020-05-09 2020-07-14 华南农业大学 Chicken essence diluent for artificial oviduct insemination and preparation method thereof
CN113940340A (en) * 2021-10-28 2022-01-18 广西富凤农牧集团有限公司 Chicken sperm diluent and preparation method and application thereof

Also Published As

Publication number Publication date
CN104396941B (en) 2016-07-06

Similar Documents

Publication Publication Date Title
CN102812950B (en) Chicken semen diluent as well as preparation method and application thereof
CN104686860B (en) A kind of nutriment extending aphidius gifuensis life span of adult
CN102578079A (en) Base fluid for diluting semens of equus animals and preparation method and use method thereof
CN103070162B (en) Muscovy duck semen diluent
CN101543209A (en) Powder for chicken sperm dilution and preservation and preparation method and application thereof
CN103719018B (en) Method for improving growth speed of ducks in embryonic phase
CN104094926A (en) Chicken semen diluent and preparation and utilization methods thereof
CN102986647B (en) Non-animal-origin frozen semen diluent
CN104322483A (en) Boar antiviral long-acting semen diluent formula and preparation method
CN104082276B (en) Sheep is containing zinc sperm dilution conserving liquid and compound method thereof
KR101457526B1 (en) Diluent Compound for Improving Pig Sperm Preservation Ability
CN104396941A (en) Chicken sperm diluting liquid and preparation method thereof
CN102726369A (en) Ultralow temperature cryopreservation application method of Epinephelus oanceolutus sperms
CN103843759B (en) Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent
CN102246747A (en) Semen diluter and preparation method as well as application method thereof
CN105145546A (en) Preserving fluid of drone semen and preparation method of preserving fluid of drone semen
CN103348967A (en) Semen diluting solution for artificial insemination of poultry and application method thereof
CN104824377A (en) Heat-stress-resistant breeding promotion composite additive for sows
CN103783032A (en) Equus animal frozen semen diluting liquid and preparation method thereof
CN103688922A (en) Equus animal semen dilution solution
CN103749433A (en) Cryopreservation diluent for equine animal seminal fluid
CN106190955A (en) A kind of method improving cow frozen semen Sperm motility and external fertilization rate
CN104068013A (en) Sheep semen dilution preserving fluid and preparation method thereof
CN111406738A (en) Chicken essence diluent for artificial oviduct insemination and preparation method thereof
CN111449058B (en) Method for improving cock semen preservation quality

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20181228

Address after: 529200 No. 10 Changxing Road, Taicheng, Taishan City, Jiangmen City, Guangdong Province (Living Building B)

Patentee after: Taishan Secco Agricultural Technology Co., Ltd.

Address before: 516898 No. 55 Xilin Road, Longmen County, Huizhou City, Guangdong Province

Patentee before: Zhang Yingqin