CN104396941A - Chicken sperm diluting liquid and preparation method thereof - Google Patents
Chicken sperm diluting liquid and preparation method thereof Download PDFInfo
- Publication number
- CN104396941A CN104396941A CN201410621015.XA CN201410621015A CN104396941A CN 104396941 A CN104396941 A CN 104396941A CN 201410621015 A CN201410621015 A CN 201410621015A CN 104396941 A CN104396941 A CN 104396941A
- Authority
- CN
- China
- Prior art keywords
- dilution
- semen
- cock
- solution
- sperm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000287828 Gallus gallus Species 0.000 title abstract description 13
- 238000007865 diluting Methods 0.000 title abstract 6
- 239000007788 liquid Substances 0.000 title abstract 6
- 238000002360 preparation method Methods 0.000 title description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- VYLVYHXQOHJDJL-UHFFFAOYSA-K cerium trichloride Chemical compound Cl[Ce](Cl)Cl VYLVYHXQOHJDJL-UHFFFAOYSA-K 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000002904 solvent Substances 0.000 claims abstract description 16
- BDKLKNJTMLIAFE-UHFFFAOYSA-N 2-(3-fluorophenyl)-1,3-oxazole-4-carbaldehyde Chemical compound FC1=CC=CC(C=2OC=C(C=O)N=2)=C1 BDKLKNJTMLIAFE-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229930091371 Fructose Natural products 0.000 claims abstract description 15
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims abstract description 15
- 239000005715 Fructose Substances 0.000 claims abstract description 15
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims abstract description 15
- 229940050906 magnesium chloride hexahydrate Drugs 0.000 claims abstract description 15
- DHRRIBDTHFBPNG-UHFFFAOYSA-L magnesium dichloride hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[Cl-].[Cl-] DHRRIBDTHFBPNG-UHFFFAOYSA-L 0.000 claims abstract description 15
- 235000013923 monosodium glutamate Nutrition 0.000 claims abstract description 15
- 239000001508 potassium citrate Substances 0.000 claims abstract description 15
- 229960002635 potassium citrate Drugs 0.000 claims abstract description 15
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 claims abstract description 15
- 235000011082 potassium citrates Nutrition 0.000 claims abstract description 15
- 235000017281 sodium acetate Nutrition 0.000 claims abstract description 15
- 229940087562 sodium acetate trihydrate Drugs 0.000 claims abstract description 15
- 229940073490 sodium glutamate Drugs 0.000 claims abstract description 15
- 238000010790 dilution Methods 0.000 claims description 63
- 239000012895 dilution Substances 0.000 claims description 63
- 210000000582 semen Anatomy 0.000 claims description 60
- 239000000243 solution Substances 0.000 claims description 53
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims description 16
- 229930182555 Penicillin Natural products 0.000 claims description 8
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 229940049954 penicillin Drugs 0.000 claims description 8
- 229960005322 streptomycin Drugs 0.000 claims description 8
- 229960002737 fructose Drugs 0.000 claims description 7
- 230000003204 osmotic effect Effects 0.000 claims description 7
- 238000012856 packing Methods 0.000 claims description 7
- 230000009027 insemination Effects 0.000 abstract description 10
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 2
- 206010061218 Inflammation Diseases 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000004054 inflammatory process Effects 0.000 abstract 1
- 210000003101 oviduct Anatomy 0.000 abstract 1
- 230000035515 penetration Effects 0.000 abstract 1
- 229940088594 vitamin Drugs 0.000 abstract 1
- 229930003231 vitamin Natural products 0.000 abstract 1
- 235000013343 vitamin Nutrition 0.000 abstract 1
- 239000011782 vitamin Substances 0.000 abstract 1
- 150000003722 vitamin derivatives Chemical class 0.000 abstract 1
- 239000003085 diluting agent Substances 0.000 description 4
- 230000002159 abnormal effect Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 230000008021 deposition Effects 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 208000007893 Salpingitis Diseases 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a chicken sperm diluting liquid. The sterile steamed water is taken as the solvent, and each 1000 mL of the diluting liquid comprises the following components by weight: 1.0 gram of magnesium chloride hexahydrate, 1.15 grams of potassium citrate, 9.5 grams of sodium acetate trihydrate, 12.5 grams of fructose, 15.2 grams of sodium glutamate, 3 grams of composite vitamin, 0.0024 to 0.0246 mg of cerium chloride, and the balance being solvent. The pH value of the chicken sperm diluting liquid is 6.8 to 7.0, and the penetration pressure is 375-380 mmol/L/kg. Compared to the conventional chicken sperm diluting liquids, the provided chicken sperm diluting liquid can improve the sperm activity, effectively prolong the sperm survival time, prominently increase the utilizing rate of breeding cocks, and reduce the phenomenon of stress reactions and oviduct inflammation of hens during the artificial insemination process.
Description
Technical field
The present invention relates to a kind of dilution, especially relate to a kind of solution for dilution of cock semen and preparation method thereof.
Background technology
Along with the universal of cage poultry and development, the artificial insemination of chicken is able to extensive popularization.The technology of artificial insemination difficulty of chicken is little, easily grasps, and equipment is simple, small investment.The artificial insemination of chicken can reduce the number of animals raised of stock cock, and during natural mating, the male and female ratio of general chicken is 10-15 only left and right, and when adopting artificial insemination, and as by inseminating weekly once, then 1 cock can join 100 even more hens.For ensureing the high fertilization rate of kind of egg in production practices, generally adopt semen deposition in 3-5 days once more.Calculate by this, during artificial insemination, the male and female ratio of chicken mostly is about 1:50.Than adopting natural mating method, can reduce by the cock number of animals raised of 80%, reducing kind of an egg cost.When adopting artificial insemination, male and female chicken is sub-cage rearing.Sub-cage rearing, can be breeding work and provides convenience, and also provides condition for supplementing the nutrients separately to cock, is conducive to improving kind of a Chicken Semen quality.When adopting artificial insemination under normal circumstances, cock all adopts single cage to raise, thus reduces and peck bucket, reduction death rate between chicken.
The realization of above-mentioned superiority mainly depends on the design of good semen diluent, screening and use.The dilution of Chicken Semen is the diluted with particular formulation.The freezen protective of Chicken Semen is still under test.To actual production, with the fresh more convenient practicality of seminal fluid semen deposition, therefore at this, preservation of Chicken Semen and dilution process are not described.Even if it should be noted that and adopt fresh semen semen deposition, after Chicken Semen extraction cock is external, if environmental temperature is too low also can affect its fertilization rate.So, when environmental temperature is lower than 20 DEG C, preferably adopt the smart cup collection essence of insulation collection.Pour into the warm water of 32 DEG C in thermos cup, if collect smart cup without special insulation during practical operation, also with additive method to the smart cup insulation of collection, such as by teat glass collection essence, can then be placed in the vessel filling warm water.Excellent semen diluent should possess the desirable living environment of sperm, and neutralization or eliminating are to the dysgenic factor of sperm.In addition, semen diluent also should have draw materials easily, configuration is simple, cost is low, be convenient to the features such as popularization.Although the research of solution for dilution of cock semen increases gradually at present, not yet there is the dilution formula that ideal so far.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of solution for dilution of cock semen that sperm viability can be made to improve, extend Sperm survival time.
Another technical problem to be solved by this invention improves a kind of method preparing above-mentioned solution for dilution of cock semen.
Technical problem to be solved by this invention is achieved by the following technical programs:
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0024 ~ 0.0246mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Preferably, described cerium chloride is 0.0036mg.
Preferably, penicillin 400,000 IU, streptomycin 400,000 IU is also comprised.
A kind of method preparing arbitrary above-mentioned solution for dilution of cock semen, it is characterized in that, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride and/or penicillin and streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The present invention has following beneficial effect: compared with existing solution for dilution of cock semen, and solution for dilution of cock semen of the present invention has: (1) improves sperm viability; (2) effectively Sperm survival time is extended; (3) utilization rate of stock cock is significantly improved; (4) reduce Breeding hens artificial insemination process produce stress, the problem such as salpingitis tubal.
Embodiment
Below in conjunction with embodiment, the present invention will be described in detail, and embodiment is only the preferred embodiment of the present invention, is not limitation of the invention.
Embodiment 1
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0024mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
Embodiment 2
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0036mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
Embodiment 3
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0246mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
Embodiment 4
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0036mg, penicillin 400,000 IU, streptomycin 400,000 IU, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride, penicillin, streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
Comparative example 1
A kind of solution for dilution of cock semen, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, penicillin 400,000 IU, streptomycin 400,000 IU, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
Prepare the method for above-mentioned solution for dilution of cock semen, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add penicillin, streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
The using method of above-mentioned solution for dilution of cock semen, is preheated to 37 DEG C by above-mentioned solution for dilution of cock semen, then mixes with Chicken Semen, and wherein the mass ratio of Chicken Semen and solution for dilution of cock semen is 1:2.
In 0 ~ 72 hour, observe the sperm viability of embodiment 1 ~ 4, comparative example 1, and measured motility rate and the abnormal rate of sperm every 24 hours, the results are shown in Table 1.
Table 1 different diluent Cord blood seminal fluid is on the impact of chicken sperm motility rate
As can be seen from Table 1, embodiment 1 ~ 4 is compared with comparative example 1, and after adding cerium chloride, the survival rate of sperm obviously extends, and the abnormal rate of sperm obviously reduces; When adding the cerium chloride of 0.0036mg, the survival rate of sperm is better, and abnormal rate is lower.
Solution for dilution of cock semen provided by the invention, because the vigor of sperm extends, makes twice Inseminated time interval extend, thus reduces the amount of labour, save labour cost.The utilization rate of stock cock can be provided simultaneously, reduce artificial insemination stress and the generation of salpingitis tubal, the laying rate of Breeding hens is provided.
Claims (4)
1. a solution for dilution of cock semen, it is characterized in that, take sterile purified water as solvent, every 1000ml dilution consists of the following composition: magnesium chloride hexahydrate 1.0g, potassium citrate 1.15g, Sodium acetate trihydrate 9.5g, fructose 12.5g, sodium glutamate 15.2g, B B-complex 3g, cerium chloride 0.0024 ~ 0.0246mg, all the other are solvent, described solution for dilution of cock semen pH value is 6.8 ~ 7.0, and osmotic pressure is 375-380mmol/L/kg.
2. solution for dilution of cock semen according to claim 1, is characterized in that, described cerium chloride is 0.0036mg.
3. solution for dilution of cock semen according to claim 1 and 2, is characterized in that, also comprises penicillin 400,000 IU, streptomycin 400,000 IU.
4. prepare the method for the arbitrary described solution for dilution of cock semen of claims 1 to 3 for one kind, it is characterized in that, aseptically, take the magnesium chloride hexahydrate of requirement, potassium citrate, Sodium acetate trihydrate, fructose, sodium glutamate, B B-complex to containing in a small amount of sterile purified water sterile beaker, dissolve, add cerium chloride and/or penicillin and streptomycin, add sterile purified water constant volume afterwards, shake all, obtain described solution for dilution of cock semen, and packing keeps for subsequent use at being placed on 3 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410621015.XA CN104396941B (en) | 2014-11-07 | 2014-11-07 | A kind of solution for dilution of cock semen and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410621015.XA CN104396941B (en) | 2014-11-07 | 2014-11-07 | A kind of solution for dilution of cock semen and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104396941A true CN104396941A (en) | 2015-03-11 |
| CN104396941B CN104396941B (en) | 2016-07-06 |
Family
ID=52634669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410621015.XA Active CN104396941B (en) | 2014-11-07 | 2014-11-07 | A kind of solution for dilution of cock semen and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104396941B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111406738A (en) * | 2020-05-09 | 2020-07-14 | 华南农业大学 | Chicken essence diluent for artificial oviduct insemination and preparation method thereof |
| CN113940340A (en) * | 2021-10-28 | 2022-01-18 | 广西富凤农牧集团有限公司 | Chicken sperm diluent and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030217380A1 (en) * | 2002-03-14 | 2003-11-20 | Rutledge Jackie J. | Composition containing gamete or embryo and animal white yolk and the use thereof |
| CN103609552A (en) * | 2013-12-06 | 2014-03-05 | 安徽五星食品股份有限公司 | Poultry semen diluent for maintaining sperm motility and prolonging insemination time |
| CN103704205A (en) * | 2014-01-15 | 2014-04-09 | 山东大学 | Bovine semen freezing dilute solution containing trace amount of rare-earth and application thereof |
-
2014
- 2014-11-07 CN CN201410621015.XA patent/CN104396941B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030217380A1 (en) * | 2002-03-14 | 2003-11-20 | Rutledge Jackie J. | Composition containing gamete or embryo and animal white yolk and the use thereof |
| CN103609552A (en) * | 2013-12-06 | 2014-03-05 | 安徽五星食品股份有限公司 | Poultry semen diluent for maintaining sperm motility and prolonging insemination time |
| CN103704205A (en) * | 2014-01-15 | 2014-04-09 | 山东大学 | Bovine semen freezing dilute solution containing trace amount of rare-earth and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张淑娟: "鸡精液不同稀释液配方筛选的研究", 《吉林农业大学硕士学位论文》 * |
| 采克俊等: "种公鸡精液稀释液的研究", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111406738A (en) * | 2020-05-09 | 2020-07-14 | 华南农业大学 | Chicken essence diluent for artificial oviduct insemination and preparation method thereof |
| CN113940340A (en) * | 2021-10-28 | 2022-01-18 | 广西富凤农牧集团有限公司 | Chicken sperm diluent and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104396941B (en) | 2016-07-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102812950B (en) | Chicken semen diluent as well as preparation method and application thereof | |
| CN104686860B (en) | A kind of nutriment extending aphidius gifuensis life span of adult | |
| CN102578079A (en) | Base fluid for diluting semens of equus animals and preparation method and use method thereof | |
| CN103070162B (en) | Muscovy duck semen diluent | |
| CN101543209A (en) | Powder for chicken sperm dilution and preservation and preparation method and application thereof | |
| CN103719018B (en) | Method for improving growth speed of ducks in embryonic phase | |
| CN104094926A (en) | Chicken semen diluent and preparation and utilization methods thereof | |
| CN102986647B (en) | Non-animal-origin frozen semen diluent | |
| CN104322483A (en) | Boar antiviral long-acting semen diluent formula and preparation method | |
| CN104082276B (en) | Sheep is containing zinc sperm dilution conserving liquid and compound method thereof | |
| KR101457526B1 (en) | Diluent Compound for Improving Pig Sperm Preservation Ability | |
| CN104396941A (en) | Chicken sperm diluting liquid and preparation method thereof | |
| CN102726369A (en) | Ultralow temperature cryopreservation application method of Epinephelus oanceolutus sperms | |
| CN103843759B (en) | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent | |
| CN102246747A (en) | Semen diluter and preparation method as well as application method thereof | |
| CN105145546A (en) | Preserving fluid of drone semen and preparation method of preserving fluid of drone semen | |
| CN103348967A (en) | Semen diluting solution for artificial insemination of poultry and application method thereof | |
| CN104824377A (en) | Heat-stress-resistant breeding promotion composite additive for sows | |
| CN103783032A (en) | Equus animal frozen semen diluting liquid and preparation method thereof | |
| CN103688922A (en) | Equus animal semen dilution solution | |
| CN103749433A (en) | Cryopreservation diluent for equine animal seminal fluid | |
| CN106190955A (en) | A kind of method improving cow frozen semen Sperm motility and external fertilization rate | |
| CN104068013A (en) | Sheep semen dilution preserving fluid and preparation method thereof | |
| CN111406738A (en) | Chicken essence diluent for artificial oviduct insemination and preparation method thereof | |
| CN111449058B (en) | Method for improving cock semen preservation quality |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181228 Address after: 529200 No. 10 Changxing Road, Taicheng, Taishan City, Jiangmen City, Guangdong Province (Living Building B) Patentee after: Taishan Secco Agricultural Technology Co., Ltd. Address before: 516898 No. 55 Xilin Road, Longmen County, Huizhou City, Guangdong Province Patentee before: Zhang Yingqin |