CN101543209A - Powder for chicken sperm dilution and preservation and preparation method and application thereof - Google Patents
Powder for chicken sperm dilution and preservation and preparation method and application thereof Download PDFInfo
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- CN101543209A CN101543209A CN200910038352A CN200910038352A CN101543209A CN 101543209 A CN101543209 A CN 101543209A CN 200910038352 A CN200910038352 A CN 200910038352A CN 200910038352 A CN200910038352 A CN 200910038352A CN 101543209 A CN101543209 A CN 101543209A
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Abstract
The invention discloses a powder for chicken sperm dilution and preservation and the preparation method and the application thereof. The powder for chicken sperm dilution and preservation comprises the following components according to the weight portion: 20-30 of glucose, 1.5-2.7 of sodium citrate, 35-45 of sodium glutamate, 55-75 of K2HPO4.3H2O, 1-5 of K2HPO4, 15-30 of trihydrate sodium acetate, 5-15 of NaCl and 1-9 of Tris; diluent is obtained after the powder is dissolved by distilled water and is adjusted in the pH value, and disinfectant can be added to the diluent. The powder for chicken sperm dilution and preservation can be applied to low-temperature long-term chicken sperm preservation or chicken sperm dilution. The invention has simple prescription component, low cost, long chicken sperm preservation time, good chicken sperm preservation effect, and high fertilization rate of hatching eggs after the chicken sperm is diluted and delivered.
Description
Technical field
The invention belongs to poultry technology of artificial insemination field, be specifically related to a kind of chicken sperm dilution and preservation powder, its preparation method and the application aspect preservation of chickens' extract liquid low-temperature long acting and dilution.
Background technology
Along with the fast development of modern aviculture, scale and intensification degree that fowl is supported by China are more and more higher, and the poultry technology of artificial insemination has also obtained using widely.
The technology of artificial insemination of chicken has not only improved the fertilization rate of hen kind egg significantly, has also greatly reduced production cost.Because the raising quantity of cock is big, semen collection frequency height, volume of semen is few, and characteristics such as sperm concentration height require the cock seminal fluid to dilute.Chicken sperm dilution can increase the seminal fluid volume simultaneously, and sperm can evenly be distributed, and is convenient to sperm after the semen deposition and moves in the hen oviduct; Semen diluent also can provide energy for sperm, guarantee to be fit to the pH environment of chickens' extract existence, keep suitable osmotic pressure and electrolyte balance, the harmful substance that neutralization and alleviation sperm and microbial metabolism produce, water down the concentration of injurious factor in the seminal plasma, prolong sperm in the time of external survival, thereby improve the fertilization rate of hen; Sperm dilution also can significantly improve kind of a cock utilization ratio, reduces the raising quantity of cock, reduces the feeding cost of kind of cock, increases economic benefit.
And, have only by the seminal fluid of dilution and could preserve, transport.The dilution technology of seminal fluid plays an important role in breeding work, the dilution technology of using seminal fluid can be avoided the problem of carrying the time-consuming trouble of cock and being unfavorable for preventing epidemic, and the cock seminal fluid of different fields, zones of different can be exchanged at any time, improve the seed selection efficient of kind of cock, give full play to the crossbreeding effect of chicken.
Chinese patent CN86101836 discloses a kind of solution for dilution of cock semen, includes glucose, sodium citrate, potassium dihydrogen phosphate, sodium hydrogen phosphate and distilled water.Its dilution was done 1: 2 and the chickens' extract liquid of dilution in 1: 1, semen deposition after preserving 7 and 24 hours respectively under 5 ℃, fertility rate of egg is respectively 94.7% and 92.2%, is suitable for semen deposition immediately after the dilution, also be suitable for doing after the dilution short-term is preserved and short-distance transport after semen deposition.But described patent does not solve the preservation technical problem of chickens' extract liquid longer time, and the anxiety of preserving the transportation that can not fundamentally solve chickens' extract liquid in 7 hours and semen deposition work is busy, requires the time of preservation longer in the production application.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, provide a kind of chicken sperm dilution and preservation to use powder, provided concrete prescription and formed, can effectively solve the technical problem that chickens' extract liquid low-temperature long acting is preserved and diluted.
Another object of the present invention provide described chicken sperm dilution and preserve with powder simple the preparation method.
A further object of the invention has provided described chicken sperm dilution and has preserved the application of using powder.
Purpose of the present invention is achieved by the following technical programs:
Provide a kind of chicken sperm dilution and preservation to use powder, comprise the component of following weight portion:
Glucose 20～30 weight portions;
Sodium citrate 1.5～2.7 weight portions;
Sodium glutamate 35～45 weight portions;
K
2HPO
4.3H
2O 55～75 weight portions;
K
2HPO
41～5 weight portion;
Sodium acetate trihydrate 15～30 weight portions;
NaCl 5～15 weight portions;
Tris (trishydroxymethylaminomethane) 1～9 weight portion.
With the chicken sperm dilution of above-mentioned preferred version with preserve to be dissolved in fully in the distilled water of 40～60 weight portions and obtain dilution with powder, be routinely added to the pharmaceutically conventional bactericide that uses according to medical science, the bactericide reference concentration is 100,000IU.Can certainly with the bactericide powder with pack in the lump after the lie said components is mixed.
The analysis pure chemistry medicine that described each component was 80 mesh sieves.
The present invention provides described chicken sperm dilution simultaneously and preserves the preparation method who uses powder, and accurately above-mentioned each component of weighing is proportionally mixed, and stirs evenly, and vacuum packaging promptly.
Described weighing, the operating temperature of mixing, stirring evenly and pack are 18～26 ℃, and relative moisture is 45～65%.
Described chicken sperm dilution and the using method of preserving with powder may further comprise the steps:
(1) packaged dilution powder is dissolved in the distilled water in proportion fully, regulates pH value and get dilution;
(2) it is stand-by dilution to be placed 37 ℃ of water-bath preheatings be directly used in dilution semen deposition or 4 ℃ of preservations.
Described dilution adopts the PH test paper to measure, and preferred pH value is 7.6～8.2.Just use when wherein distilled water can begin prepared and diluted liquid half an hour before sperm dilution.
Described chicken sperm dilution and preservation are mixed with the dilution that dilution can be applicable to preservation of chickens' extract liquid low-temperature long acting or chickens' extract liquid with powder.
The invention has the beneficial effects as follows:
The invention provides a kind of new good low temperature dilution prescription, prolong the holding time of seminal fluid effectively, to enlarge the utilization ratio of good species cock; And by semen deposition test behind the chicken sperm dilution, observe, determine to be fit to the chicken sperm dilution formula of liquid of large-scale production needs the fertilization rate of hen kind egg, the influence of emergence rate, and then the fertilization rate of raising kind of egg, reduce the cock number of animals raised, reduce production costs, significantly increase economic benefit.
The present invention is by a large amount of experiment confirms, and chickens' extract liquid is preserved down in 4 ℃ through diluted of the present invention and packing, and sperm viability is more than 0.7 after 6 days; Simultaneously, the semen deposition result of the test behind the chicken sperm dilution shows that fertilization rate and emergence rate can reach about 94% and 93.5% respectively, and than the better effects if of former smart semen deposition, can satisfy produce required; In addition, the fertilization rate difference of semen deposition and former smart control group is not remarkable behind the dilution seminal fluid low temperature preservation 6h, shows that the preservation back semen deposition of dilution seminal fluid short time can be applied to produce, and can not influence fertility rate of hatching egg.So just can carry out that the morning semen collection is preserved, the semen deposition in afternoon, can improve to produce and go up the busy of semen deposition at once after the habitual semen collection in afternoon, alleviate workload to a certain extent, make things convenient for production.
Dilution preparation method of the present invention is simple, and cost is lower, is fit to production application and popularization.
The present invention has enriched the achievement in research of chicken sperm dilution and low temperature preservation aspect, can further promote the Animal Genetics, Breeding and Reproduction subject development.
Embodiment
Further describe the present invention below in conjunction with specific embodiment.
The dilution powder prescription that embodiment 1 provides according to table 1, prepare raw material:
Table 1 dilution powder prescription
| Reagent | Content |
| Glucose | 0.2400g |
| Sodium citrate | 0.0250g |
| Sodium glutamate | 0.4005g |
| K 2HPO 4.3H 2O | 0.62055g |
| K 2HPO 4 | 0.0325g |
| Sodium acetate trihydrate | 0.2050g |
| NaCl | 0.0900g |
| Tris | 0.0415g |
| Penicillin | 0.0100g |
Various chemicalss (analyzing pure) in the buying prescription, bulky grain or the composition that lumpd need be through fragmentation and the 80 mesh sieve formation powder that sieves.
Accurately the various compositions of weighing mix according to the ratio in the prescription, and do to stir fully, are packaged into bag then, vacuumize and seal to obtain 1 bag chicken sperm dilution powder.
Above process generally requires temperature to be controlled at 18～26 ℃, and relative moisture is controlled at 45～65%.
Provide 1 bag chicken sperm dilution powder in the table 1 and can be equipped with 50ml distilled water and dissolve fully, adopting the PH test paper to survey pH value is 7.6.
Above-mentioned chicken sperm dilution powder is pressed the difference of Packing Unit can be through two steaming dissolved in distilled water to 200ml, 500ml or 1000ml etc., constantly be stirred to dissolving fully through magnetic stirrer then, obtain dilution, put into 37 ℃ of water-bath preheatings, to satisfy the different demands of different scales chicken house.
Usually begin prepared and diluted liquid half an hour before sperm dilution.
Can be directly used in semen deposition according to the ratio of the 1:1 seminal fluid after with chicken sperm dilution, also can 4 ℃ of preservations stand-by.
The dilution powder prescription that embodiment 2 provides according to table 2, prepare raw material:
Table 2 dilution powder prescription
| Reagent | Content |
| Glucose | 0.2000g |
| Sodium citrate | 0.0150g |
| Sodium glutamate | 0.4500g |
| K 2HPO 4.3H 2O | 0.5500g |
| K 2HPO 4 | 0.0500g |
| Sodium acetate trihydrate | 0.3000g |
| NaCl | 0.0500g |
| Tris | 0.0900g |
| Chloramphenicol | 0.0100g |
Other operations are with embodiment 1.Be equipped with 60ml distilled water and dissolve the back fully to transfer pH value be 8.0 seminal fluid to be added directly semen deposition after the diluted according to 1:2, also can 4 ℃ of preservations stand-by.
The dilution powder prescription that embodiment 3 provides according to table 3, prepare raw material:
Table 3 dilution powder prescription
| Reagent | Content |
| Glucose | 0.3000g |
| Sodium citrate | 0.0270g |
| Sodium glutamate | 0.3500g |
| K 2HPO 4.3H 2O | 0.7500g |
| K 2HPO 4 | 0.0100g |
| Sodium acetate trihydrate | 0.3000g |
| NaCl | 0.1500g |
| Tris | 0.0100g |
| Streptomycin | 0.0100g |
Various chemicalss (analyzing pure) in the buying prescription, bulky grain or the composition that lumpd need be through fragmentation and the 80 mesh sieve formation powder that sieves.Other operations are with embodiment 1.The directly semen deposition of seminal fluid after the dilution, also can 4 ℃ of preservations stand-by.
Embodiment 4 adopts embodiment 1～3 described dilution that chickens' extract liquid is tested according to the dilution proportion of 1:1 and the sperm viability situation detection after preserving under 4 ℃.
Embodiment:
Get chicken mixed semen of the same race and dilute, and, observed sperm viability every 24 hours, record vigor situation of change in 4 ℃ of preservations through dilution 1:1 of the present invention.The results are shown in Table shown in 4:
Table 4 dilution seminal fluid low temperature (4 ℃) is preserved the variation of vigor
As can be seen from Table 4, preserve 144 hours (6 days) back sperm viability, show that dilution prescription of the present invention is fine to the effect of chicken sperm dilution preservation still more than 0.7.
Embodiment 5 dilutions of the present invention are preserved the influence test of vigor to different cultivars chickens' extract liquid low temperature (4 ℃)
Embodiment:
Get the cock seminal fluid of five kinds of different cultivars, press the 1:1 dilution proportion with the embodiment of the invention 1 dilution respectively, and, observed sperm viability every 24 hours, record vigor situation of change in 4 ℃ of preservations.The result is as shown in table 5:
The vigor that the different cock seminal fluid of table 5 are preserved with this prescription dilution back low temperature compares
| Holding time (h) | The soil chicken | The fiber crops chicken | Yellow 4 | Hong Kong chicken | Short F |
| 24 | 0.98 | 0.98 | 0.98 | 0.97 | 0.98 |
| 48 | 0.97 | 0.97 | 0.97 | 0.96 | 0.97 |
| 72 | 0.96 | 0.95 | 0.96 | 0.95 | 0.96 |
| 96 | 0.94 | 0.93 | 0.95 | 0.94 | 0.95 |
| 120 | 0.93 | 0.93 | 0.94 | 0.93 | 0.93 |
| 144 | 0.85 | 0.85 | 0.90 | 0.87 | 0.90 |
| 168 | 0.80 | 0.75 | 0.80 | 0.75 | 0.80 |
As can be seen from Table 5, different cultivars cock seminal fluid is through this diluted formulations dilution, and low temperature is preserved the vigor that all had after (7 days) more than 0.7 in 168 hours, shows that this prescription is all fine to the effect of different cultivars chicken sperm dilution preservation, can apply.
Embodiment 6 adopts the seminal fluid semen deposition after the diluted of the present invention that the influence of hen fertility rate of hatching egg, emergence rate is tested
The preparation of dilution is with reference to embodiment 1.
Embodiment:
Gather the cock fresh semen, after this dilution 1:1 dilution, at once the test hen carried out semen deposition, and compare (two groups of insemination doses are identical), observe fertilization rate, the emergence rate of hen kind egg, the results are shown in Table shown in 6 with former smart semen deposition:
The comparison of hen fertility rate of hatching egg, emergence rate after table 6 dilution seminal fluid semen deposition and the former smart semen deposition
| Experimental group | Go into incubation | The light egg | Dead germ | Weak embryo | Fertile egg | The number of emerging | Fertilization rate % | Emergence rate % |
| The dilution group | 8283 | 568 | 230 | 89 | 7715 | 7209 | 93.14 a | 93.44 a |
| Control group | 7503 | 520 | 295 | 80 | 6983 | 6394 | 93.07 a | 91.57 b |
Annotate: represent difference not significantly (p〉0.05), shoulder mark different letter representation significant differences (p≤0.05) with shoulder mark same letter in the column of figure
As can be seen from Table 6, the fertilization rate of dilution group is higher than former smart control group, but difference is not remarkable; The emergence rate of dilution group is significantly higher than former smart control group.
Chickens' extract liquid low temperature (4 ℃) after embodiment 7 diluted of the present invention is preserved the influence test of back semen deposition to fertility rate of hatching egg
Embodiment:
The preparation of dilution and using method are with reference to embodiment 1.
The morning, 9 semen collections, 1:1 diluted back 4 ℃ of preservation 6h, and 3 semen depositions that afternoon contrast with former smart semen deposition simultaneously, hen fertility rate of hatching egg after the observation semen deposition, and the result is as follows:
The comparison of semen deposition and former smart semen deposition fertilization rate behind the table 7 dilution seminal fluid preservation 6h
| Experimental group | Go into incubation | The light egg | Dead germ | Weak embryo | Fertile egg | Fertilization rate % |
| Preserve the 6h semen deposition | 1794 | 103 | 42 | 22 | 1691 | 94.26 a |
| Former smart contrast | 10076 | 471 | 276 | 100 | 9605 | 95.33 a |
Annotate: represent difference not significantly (p〉0.05) with shoulder mark same letter in the column of figure
As can be seen from Table 7, preserve 6h after semen deposition not remarkable with the fertilization rate difference of former smart control group, show the dilution seminal fluid preserve 6h after semen deposition can be applied to production, can not influence fertility rate of hatching egg.So just can carry out that the morning semen collection is preserved, the semen deposition in afternoon, can improve to produce and go up the busy of semen deposition at once after the habitual semen collection in afternoon, alleviate workload to a certain extent, make things convenient for production.
Claims (8)
1, a kind of chicken sperm dilution and preservation powder is characterized in that comprising the component of following weight portion:
Glucose 20～30 weight portions;
Sodium citrate 1.5～2.7 weight portions;
Sodium glutamate 35～45 weight portions;
K
2HPO
43H
2O 55～75 weight portions;
K
2HPO
41～5 weight portion;
Sodium acetate trihydrate 15～30 weight portions;
NaCl 5～15 weight portions;
Tris 1～9 weight portion.
2,, it is characterized in that also comprising pharmaceutically conventional sterilization agent component according to described chicken sperm dilution of claim 1 and preservation powder.
3,, it is characterized in that the analysis pure chemistry medicine that described each component was 80 mesh sieves according to claim 1 or 2 described chicken sperm dilutions and preservation powder.
4, a kind of claim 1 or 2 described chicken sperm dilutions and preservation is characterized in that each component of accurate weighing with the preparation method of powder, proportionally mix, and stir evenly, and vacuum packaging promptly.
5, according to the preparation method of described chicken sperm dilution of claim 4 and preservation usefulness powder, the operating temperature that it is characterized in that described weighing, mixes, stirs evenly and pack is 18～26 ℃, and relative moisture is 45～65%.
6, a kind of claim 1 or 2 described chicken sperm dilutions and the preservation using method of powder is characterized in that may further comprise the steps:
(1) packaged dilution powder is dissolved in the distilled water in proportion fully, regulates pH value and get dilution;
(2) it is stand-by dilution to be placed 37 ℃ of water-bath preheatings be directly used in dilution semen deposition or 4 ℃ of preservations.
7, according to the using method of described chicken sperm dilution of claim 6 and preservation usefulness powder, it is characterized in that the described pH value of step (1) is 7.6～8.2.
8, a kind of claim 1 or 2 described chicken sperm dilutions and preservation is characterized in that being mixed with the dilution that dilution is applied to preservation of chickens' extract liquid low-temperature long acting or chickens' extract liquid with the application of powder.
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| CN200910038352A CN101543209A (en) | 2009-03-31 | 2009-03-31 | Powder for chicken sperm dilution and preservation and preparation method and application thereof |
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|---|---|---|---|
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246747A (en) * | 2011-06-23 | 2011-11-23 | 北京市华都峪口禽业有限责任公司 | Semen diluter and preparation method as well as application method thereof |
| CN103070162A (en) * | 2013-01-30 | 2013-05-01 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
| CN103843759A (en) * | 2014-03-07 | 2014-06-11 | 四川农业大学 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
| CN104094926A (en) * | 2014-08-05 | 2014-10-15 | 燕海峰 | Chicken semen diluent and preparation and utilization methods thereof |
| CN104336006A (en) * | 2014-10-15 | 2015-02-11 | 隆安凤鸣农牧有限公司 | Semen diluent and method for artificial insemination of breeding hens at interval of six days |
| CN104814005A (en) * | 2015-03-26 | 2015-08-05 | 谢光玉 | Preservation method for seminal fluid of Schizothorax davidi |
| CN105287045A (en) * | 2015-10-15 | 2016-02-03 | 燕海峰 | Semen diluting method for artificial insemination in breeding chicken farm |
| CN108566945A (en) * | 2017-06-26 | 2018-09-25 | 常州市四季禽业有限公司 | Yellow-feather broiler semen diluent and its preparation and application |
| CN108887261A (en) * | 2018-07-04 | 2018-11-27 | 上海市动物疫病预防控制中心 | A kind of pheasant semen diluent |
| CN110946132A (en) * | 2019-12-31 | 2020-04-03 | 赵章成 | Poultry semen nutrient, nutrient diluent, preparation method and application thereof |
| CN110973120A (en) * | 2019-12-25 | 2020-04-10 | 西北农林科技大学 | Preparation method of Muscovy duck semen normal temperature preservation diluent |
| CN114208815A (en) * | 2021-12-30 | 2022-03-22 | 广西富凤农牧集团有限公司 | Sperm diluent suitable for large-scale poultry farm and preparation method thereof |
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2009
- 2009-03-31 CN CN200910038352A patent/CN101543209A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246747A (en) * | 2011-06-23 | 2011-11-23 | 北京市华都峪口禽业有限责任公司 | Semen diluter and preparation method as well as application method thereof |
| CN103070162B (en) * | 2013-01-30 | 2014-10-08 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
| CN103070162A (en) * | 2013-01-30 | 2013-05-01 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
| CN103843759B (en) * | 2014-03-07 | 2015-05-20 | 四川农业大学 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
| CN103843759A (en) * | 2014-03-07 | 2014-06-11 | 四川农业大学 | Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent |
| CN104094926A (en) * | 2014-08-05 | 2014-10-15 | 燕海峰 | Chicken semen diluent and preparation and utilization methods thereof |
| CN104336006A (en) * | 2014-10-15 | 2015-02-11 | 隆安凤鸣农牧有限公司 | Semen diluent and method for artificial insemination of breeding hens at interval of six days |
| CN104814005A (en) * | 2015-03-26 | 2015-08-05 | 谢光玉 | Preservation method for seminal fluid of Schizothorax davidi |
| CN105287045A (en) * | 2015-10-15 | 2016-02-03 | 燕海峰 | Semen diluting method for artificial insemination in breeding chicken farm |
| CN105287045B (en) * | 2015-10-15 | 2017-01-04 | 燕海峰 | A kind of semen dilution method that can be used for kind of chicken house artificial insemination |
| CN108566945A (en) * | 2017-06-26 | 2018-09-25 | 常州市四季禽业有限公司 | Yellow-feather broiler semen diluent and its preparation and application |
| CN108887261A (en) * | 2018-07-04 | 2018-11-27 | 上海市动物疫病预防控制中心 | A kind of pheasant semen diluent |
| CN110973120A (en) * | 2019-12-25 | 2020-04-10 | 西北农林科技大学 | Preparation method of Muscovy duck semen normal temperature preservation diluent |
| CN110946132A (en) * | 2019-12-31 | 2020-04-03 | 赵章成 | Poultry semen nutrient, nutrient diluent, preparation method and application thereof |
| CN114208815A (en) * | 2021-12-30 | 2022-03-22 | 广西富凤农牧集团有限公司 | Sperm diluent suitable for large-scale poultry farm and preparation method thereof |
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Application publication date: 20090930 |