CN103843759A - Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent - Google Patents
Chicken seminal fluid diluent applicable to high altitude localities and preparation method and application of diluent Download PDFInfo
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Abstract
The invention relates to chicken seminal fluid diluent applicable to high altitude localities. The chicken seminal fluid diluent is composed of a solution A and a solution B in a volume ratio of 50: (5-6), wherein the solution A is prepared from the following components: 8-9 grams of sodium acetate trihydrate, 18-22 grams of sodium glutamate monohydrate, 9-10 grams of sodium chloride, 10-15 grams of glucose and 10-15 grams of sodium citrate based on each liter of purified water; the solution B is prepared from the following components: 2 grams of sodium percarbonate and 2ml of glycerinum with the volume percentage of 5% based on each liter of purified water. The invention also provides a particular application method of the chicken seminal fluid diluent applicable to high altitude localities. Compared with an existing chicken seminal fluid diluent, the chicken seminal fluid diluent disclosed by the invention has the advantages of being better applicable to anoxic and cold environments of the high altitude localities, and being suitable for immediate insemination of the diluted seminal fluid and also suitable for insemination of diluted seminal fluid after short-period storage and short-distance transportation.
Description
Technical field
The present invention relates to poultry farming, particularly relate to a kind of chickens' extract liquid that is suitable for high altitude localities and release thin liquid and preparation method thereof and application.
Background technology
Hide chicken, be distributed widely in the very low original chicken breed in place of artificial selection degree of high altitude localities (1500 meters～3000 meters), in China's raising history of existing more than 1000 year.Hide chicken build length and short legs, head height tail is low, chest muscle prosperity, and property is active, more neurotic, bellicose, is apt to ascend a height and circle in the air.Hide chicken matter extremely delicious, cultivation scale is less, is applicable to very much high-end consumption market.Having at present a most important factor to cause Tibetan chicken to be difficult to enter high-end consumption market, is to hide chicken sum less.
The present age, cultural technique, relied on heredity selection to improve the egg production of chicken, relied on artificial fertilization technology greatly to improve the fertilization rate of chicken, thereby improved incubation rate, expand the colony of chicken, thereby the quantity of raising chicken was supplied market.
The lack of quantity of spermatic fluid of cock, sperm concentration is large, and the seminal fluid of at every turn adopting can only be given the semen deposition of minority hen, can expand corresponding amount to hen semen deposition with solution for dilution of cock semen dilution, improves the availability of good cock, has leading role for chicken group's breeding.The solution for dilution of cock semen material that can also supplement the nutrients, keeps osmotic pressure that seminal fluid is suitable and the variation of buffering seminal fluid pH.Cock spermatozoa can keep for a long time fertility in hen body, and be easy in vitro lose fertility, and its reason is in the time of semen collection, the various compositions of cloaca body of gland are sneaked among seminal fluid, cause sperm motility quickening, energy to reduce fast, and cause fertility to reduce.Suitable dilution can be used as the nutrients source of makeup energy.Sperm produces and has accumulated carbonic acid gas and lactic acid in metabolic process, and the pH of seminal fluid is reduced, and some enzyme of sperm is suppressed, and causes the fertility of sperm to reduce, even dead.Use in energy and the dilution of lactic acid, can reach the effect of buffering.Application dilution means, can also keep the suitable osmotic pressure of seminal fluid and electrolytical balance.
So why, hide the still quantity rareness of chicken? the one, because hide chicken major part without seed selection, egg production is low, and the 2nd, the fertilization rate of the artificial insemination of high altitude localities is lower.And one of lower basic reason of the fertilization rate of the artificial insemination of high altitude localities is in actual production, to lack a kind of solution for dilution of cock semen that is applicable to high altitude localities.
Summary of the invention
The object of this invention is to provide a kind of chickens' extract liquid that is suitable for high altitude localities and release thin liquid and preparation method thereof and application.
The invention provides a kind of chickens' extract liquid that is suitable for high altitude localities and release thin liquid, is 50:(5-6 by volume ratio) solution A and solution B form;
Described solution A is made up of following composition: in every liter of pure water, add Sodium acetate trihydrate 8-9 gram, 18-22 gram of sodium glutamate monohydrate, sodium chloride 9-10 gram, glucose 10-15 gram, sodium citrate 10-15 gram;
Described solution B is made up of following composition: the glycerine 2ml that adds 2 grams of SODIUM PERCARBONATE, volumn concentration 5% in every liter of pure water.
Described solution A is preferably as follows composition and makes: in every liter of pure water, add 9 grams of Sodium acetate trihydrates, 20 grams of sodium glutamate monohydrates, 10 grams, sodium chloride, 15 grams of glucose, 12 grams of sodium citrates.
The chickens' extract liquid that is suitable for high altitude localities described in the present invention also provides is released the application of thin liquid in the artificial insemination of high altitude localities chicken.Described chicken is preferably hidden chicken.
The concrete steps of described application are as follows:
1) obtain solution A in advance; While gathering chickens' extract liquid, 30 minutes ahead of time configuration solution B; Mixed solution A and solution B obtain being suitable for the chickens' extract liquid of high altitude localities and release thin liquid, are heated to 40 DEG C of insulations, regulate pH value to be 6.8-7.2, for subsequent use;
2) gather chickens' extract liquid, then according to volume ratio 1:(2-8) ratio release thin liquid dilution with the chickens' extract liquid of step 1) standby be suitable for high altitude localities, obtain the chickens' extract liquid after dilution;
3) step 2) semen deposition after chickens' extract liquid semen deposition immediately after gained dilution or short-term preservation; When semen deposition, the chickens' extract liquid 0.1ml getting after dilution splashes in hen cloaca.
Wherein, obtain solution A described in step 1), for add Sodium acetate trihydrate, sodium glutamate monohydrate, sodium chloride, glucose, sodium citrate in pure water, low-speed centrifugal 5 minutes, obtains solution A.
The speed of described low-speed centrifugal is 1500～3000 revs/min.
Wherein, obtain solution B described in step 1), for add the glycerine of SODIUM PERCARBONATE, volumn concentration 5% in pure water, mixes and obtains solution B.
Wherein, mixed solution A and solution B described in step 1), for according to solution A: the volume ratio of solution B is 50:(5-6) ratio, limit is rocked solution A limit and is added solution B, mixes the chickens' extract liquid that obtains being suitable for high altitude localities and releases thin liquid.
Wherein, regulate pH value described in step 1), be preferably and be adjusted to 7.0.
Wherein, short-term preservation described in step 3), the holding time is 24 hours at most.
Wherein, short-term preservation described in step 3), the temperature of preservation is 0-5 DEG C.
Wherein, short-term preservation described in step 3), in satisfactory situation of temperature and holding time, can do and transport guaranteeing.
The present invention has following beneficial effect:
Solution for dilution of cock semen of the present invention is compared with existing solution for dilution of cock semen, can better adapt to the environment of high altitude localities anoxic, severe cold (low temperature).This be due to: adopted SODIUM PERCARBONATE and glycerine, the former can produce oxygen in the aqueous solution, the latter can extend time of retaining in solution of oxygen that SODIUM PERCARBONATE produces under the hypobaric condition in high altitude localities, promote the dissolved oxygen content of solution B, and in the time will gathering chickens' extract liquid, within 30 minutes ahead of time, configuration solution B has been avoided the problem of overflowing due to long dissolved oxygen of resting period, the effect reaching be join the interior dissolved oxygen content of solution for dilution of cock semen high.Carry out the artificial insemination of chicken with solution for dilution of cock semen of the present invention in high altitude localities, than using existing solution for dilution of cock semen to promote dissolved oxygen content, greatly improved fertility rate and hatchability.
Make the chickens' extract liquid of 1:2 dilution with semen diluent of the present invention, in high altitude localities, semen deposition immediately and preserve semen deposition after 24 hours at 4 DEG C, the incubation rate of hiding chicken is respectively 79% and 62%, significantly higher than the fertility rate of egg (48%) of former essence semen deposition immediately.The present invention is suitable for semen deposition immediately after dilution, is also suitable for doing semen deposition after short-term preservation (in 24 hours) and short-distance transport after dilution.Semen diluent composition of the present invention is few, and preparation is simple, draws materials easily, cheap, is easy to promote.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.2 grams of embodiment Sodium acetate trihydrates used, sodium glutamate monohydrate, sodium chloride, glucose, sodium citrate, SODIUM PERCARBONATE, 5% glycerine are all opened reagent company purchased from Yaan Rui Jinte company and Yaan.
Embodiment 1 prepares the chickens' extract liquid that the present invention is suitable for high altitude localities and releases thin liquid 1
Solution A is made up of following composition: in every liter of pure water, add 9 grams of Sodium acetate trihydrates, 20 grams of sodium glutamate monohydrates, 10 grams, sodium chloride, 15 grams of glucose, 12 grams of sodium citrates;
Solution B is made up of following composition: the glycerine 2ml that adds 2 grams of SODIUM PERCARBONATE, volumn concentration 5% in every liter of pure water.
According to solution A: the volume ratio of solution B is that 10:1(is 50:5) ratio, limit is rocked solution A limit and is added solution B, mixes the chickens' extract liquid that obtains being suitable for high altitude localities and releases thin liquid 1.
Embodiment 2 prepares the chickens' extract liquid that the present invention is suitable for high altitude localities and releases thin liquid 2
Solution A is made up of following composition: in every liter of pure water, add 8 grams of Sodium acetate trihydrates, 22 grams of sodium glutamate monohydrates, 10 grams, sodium chloride, 15 grams of glucose, 15 grams of sodium citrates;
Solution B is made up of following composition: the glycerine 2ml that adds 2 grams of SODIUM PERCARBONATE, volumn concentration 5% in every liter of pure water.
According to solution A: the ratio that the volume ratio of solution B is 50:6, limit is rocked solution A limit and added solution B, mixes the chickens' extract liquid that obtains being suitable for high altitude localities and releases thin liquid 2.
The chickens' extract liquid that embodiment 3 the present invention are suitable for high altitude localities is released the extension rate of thin liquid
Described the present invention is suitable for the chickens' extract liquid of high altitude localities and releases thin liquid according to the formula of embodiment 1, obtain solution A in advance; While gathering chickens' extract liquid, 30 minutes ahead of time configuration solution B; Mixed solution A and solution B obtain being suitable for the chickens' extract liquid of high altitude localities and release thin liquid, are heated to 40 DEG C of insulations, adjust PH and be 7.0(regulate carry out with the HCl solution of mass percent 1-2% or the NAOH solution of mass percent 1-2%), for subsequent use.
8 extension rates are set, are specially 1:1(and get the seminal fluid that volume is V, adding volume is that the chickens' extract liquid of the present invention of V is released thin liquid and diluted, lower with), 1:2,1:3,1:4,1:5,1:6,1:7,1:8.
Test site is in Xiangcheng County, be positioned at the west and south, Tibetan Autonomous Prefecture of Garze, Sichuan Province, 2865 meters of height above sea level, 16 DEG C of temperature when test, adopt hen in the layer chicken phase of raising 240 days, 3 hens of each ratio, adopt after the seminal fluid of same cock, diluting respectively according to extension rate, give at twice hen semen deposition, is once semen deposition immediately after handling, semen deposition after another time preserved 24 hours under 4 DEG C of environment, adopt the insulation that uses the same method for every group, every hen semen deposition 0.1ml(of semen deposition drips 1 with glue head dropper), every hen is collected about 6～9 of egg.The collected same time of kind egg of twice semen deposition is detected fertilization rate by the method for " according to egg ", the results are shown in Table 1.
The fertilization rate of the different dilution ratios of table 1
| Chickens' extract liquid and dilution ratio | 1:1 | 1:2 | 1：3 | 1：4 | 1：5 | 1：6 | 1：7 | 1：8 |
| Fertilization rate % after semen deposition immediately | 70 | 79 | 77 | 74 | 71 | 70 | 68 | 68 |
| Fertilization rate % after semen deposition in 24 hours | 52 | 62 | 53 | 37 | 33 | 28 | 22 | 19 |
Result shows, dilution 1:(1-8) all can obtain good effect, especially dilution ratio and be 1:2 time, the fertilization rate after semen deposition is that 79%, 24 hour fertilization rate after semen deposition is 62% immediately.
The chickens' extract liquid that embodiment 4 the present invention are suitable for high altitude localities is released the application of thin liquid
Test site is in Xiangcheng County, be positioned at the west and south, Tibetan Autonomous Prefecture of Garze, Sichuan Province, 2865 meters of height above sea level, 16 DEG C of temperature when test, 200 of hens in the employing raising layer chicken phase of 240 days, 16 of cocks, be divided into 4 groups, 4 of every group of cocks, 50 of hens, whole process of the test is by same semen collection person's semen collection, and cock extraction sperm volume is 0.3ml to 2ml.After extraction seminal fluid according to every group of processing method to seminal fluid, at twice to 25 hen semen depositions of this group, it is once semen deposition immediately after handling, semen deposition after another time preserved 24 hours under 4 DEG C of environment, adopt the insulation that uses the same method for every group, every hen semen deposition 0.1ml(of semen deposition drips 1 with glue head dropper), in method of operating, only difference is the dilution difference of seminal fluid.The collected same time of kind egg of twice semen deposition is detected fertilization rate by the method for " according to egg ".
The dilution of different seminal fluid is processed as follows:
(1) not add semen diluent as contrast.
(2) add physiological saline (get the seminal fluid that volume is V, adding volume is that the physiological saline of 2V dilutes, lower with) according to 1:2.Physiological saline is provided by poultry scientific research breeding factory of Sichuan Agricultural University.
(3) add common semen diluent according to 1:2, common semen diluent is provided by poultry scientific research breeding factory of Sichuan Agricultural University, and formula is in 1 premium on currency, to add 9 grams, sodium chloride, 22 grams of glucose, 15 grams of sodium citrates.
(4) the chickens' extract liquid that adds the present invention to be suitable for high altitude localities according to 1:2 is released thin liquid, and described the present invention is suitable for the chickens' extract liquid of high altitude localities and releases thin liquid according to the formula of embodiment 1, obtain solution A in advance; While gathering chickens' extract liquid, 30 minutes ahead of time configuration solution B; Mixed solution A and solution B obtain being suitable for the chickens' extract liquid of high altitude localities and release thin liquid, are heated to 40 DEG C of insulations, adjust PH and are 7.0, for subsequent use.
Every group of seminal fluid processing method and incubation rate the results are shown in Table 2:
Fertilization rate after the semen deposition of table 2 different diluent
This test, in process of the test, is processed (1), and because seminal fluid does not pass through any processing, collecting semen amount is less, and underweight is to complete test, so caused fertilization rate lower.The former seminal fluid of chicken, as without any processing, in outside air, just loses activity after common 6 hours.So fertilization rate is 0 after 24 hours.
Adopt the fertilization rate (62%) of fertilization rate (79%) after dilution of the present invention semen deposition immediately and semen deposition after 24 hours all higher than other processing.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. the chickens' extract liquid that is suitable for high altitude localities is released a thin liquid, it is characterized in that, it is 50:(5-6 by volume ratio that this chickens' extract liquid is released thin liquid) solution A and solution B form;
Described solution A is made up of following composition: in every liter of pure water, add Sodium acetate trihydrate 8-9 gram, 18-22 gram of sodium glutamate monohydrate, sodium chloride 9-10 gram, glucose 10-15 gram, sodium citrate 10-15 gram;
Described solution B is made up of following composition: the glycerine 2ml that adds 2 grams of SODIUM PERCARBONATE, volumn concentration 5% in every liter of pure water.
2. the chickens' extract liquid that is suitable for high altitude localities claimed in claim 1 is released thin liquid, it is characterized in that, described solution A is made up of following composition: in every liter of pure water, add 9 grams of Sodium acetate trihydrates, 20 grams of sodium glutamate monohydrates, 10 grams, sodium chloride, 15 grams of glucose, 12 grams of sodium citrates.
3. the chickens' extract liquid that is suitable for high altitude localities as described in claim 1 or 2 any one is released the application of thin liquid in the artificial insemination of high altitude localities chicken.
4. application claimed in claim 3, is characterized in that, concrete steps are as follows:
1) obtain solution A in advance; While gathering chickens' extract liquid, 30 minutes ahead of time configuration solution B; Mixed solution A and solution B obtain being suitable for the chickens' extract liquid of high altitude localities and release thin liquid, are heated to 40 DEG C of insulations, regulate pH value to be 6.8-7.2, for subsequent use;
2) gather chickens' extract liquid, then according to volume ratio 1:(2-8) ratio release thin liquid dilution with the chickens' extract liquid of step 1) standby be suitable for high altitude localities, obtain the chickens' extract liquid after dilution;
3) step 2) semen deposition after chickens' extract liquid semen deposition immediately after gained dilution or short-term preservation; When semen deposition, the chickens' extract liquid 0.1ml getting after dilution splashes in hen cloaca.
5. application claimed in claim 4, is characterized in that, obtain solution A described in step 1), and for add Sodium acetate trihydrate, sodium glutamate monohydrate, sodium chloride, glucose, sodium citrate in pure water, low-speed centrifugal 5 minutes, obtains solution A.
6. application claimed in claim 5, is characterized in that, the speed of described low-speed centrifugal is 1500～3000 revs/min.
7. application described in claim 4, is characterized in that, obtain solution B described in step 1), for add the glycerine of SODIUM PERCARBONATE, volumn concentration 5% in pure water, mixes and obtain solution B.
8. application described in claim 4, it is characterized in that, mixed solution A and solution B described in step 1), for according to solution A: the volume ratio of solution B is 50:(5-6) ratio, limit is rocked solution A limit and is added solution B, mixes the chickens' extract liquid that obtains being suitable for high altitude localities and releases thin liquid.
9. application described in claim 4, is characterized in that, regulates pH value described in step 1), for being adjusted to 7.0.
10. application described in claim 4, is characterized in that, short-term preservation described in step 3), and the holding time is 24 hours at most, the temperature of preservation is 0-5 DEG C.
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Cited By (4)
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| CN105287045A (en) * | 2015-10-15 | 2016-02-03 | 燕海峰 | Semen diluting method for artificial insemination in breeding chicken farm |
| CN106175977A (en) * | 2016-09-20 | 2016-12-07 | 成都测迪森生物科技有限公司 | A kind chicken sperm extractor tool |
| CN106420104A (en) * | 2016-09-20 | 2017-02-22 | 四川农业大学 | Electric shock semen collection cup for chicken |
| CN107913118A (en) * | 2017-12-19 | 2018-04-17 | 佛山科学技术学院 | A kind of mule duck artificial insemination method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106420104A (en) * | 2016-09-20 | 2017-02-22 | 四川农业大学 | Electric shock semen collection cup for chicken |
| CN106175977B (en) * | 2016-09-20 | 2017-12-19 | 上海丰蓓生物科技有限公司 | A kind of breeder sperm extractor tool |
| CN107913118A (en) * | 2017-12-19 | 2018-04-17 | 佛山科学技术学院 | A kind of mule duck artificial insemination method |
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