CN104814005A - Preservation method for seminal fluid of Schizothorax davidi - Google Patents
Preservation method for seminal fluid of Schizothorax davidi Download PDFInfo
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- CN104814005A CN104814005A CN201510135862.XA CN201510135862A CN104814005A CN 104814005 A CN104814005 A CN 104814005A CN 201510135862 A CN201510135862 A CN 201510135862A CN 104814005 A CN104814005 A CN 104814005A
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Abstract
The invention provides a preservation method for the seminal fluid of Schizothorax davidi. The method comprises the following steps: acquisition of the seminal fluid; preparation of a diluent; dilution of the seminal fluid; and refrigeration of the seminal fluid. With the method provided by the invention, the utilization rate of the sperms of Schizothorax davidi and outbreeding fertilization rate of Schizothorax davidi are improved; moreover, requirements on conditions are low, operation can be easily realized, and the method is applicable to large-scale breeding.
Description
Technical field
The present invention relates to fish reproduction technology, belong to technical field of aquaculture, be specifically related to Schizothorax davidi Semen routine method.
Background technology
Schizothorax davidi (Schizothorax (Racoma) davidi) belongs to Cypriniformes, Cyprinidae, Schizothoracinae, Schizothorax, splits buttocks fish subgenus.Be commonly called as: refined fish, Chong Kou, heavy mouth brachymystax lenok, heavy lip brachymystax lenok, thin soft-shelled turtle.English name: David ' s schizothoracin.Be distributed in the Heavenly Stems and Earthly Branches stream of the Changjiang river, especially many to see in the river, valley of Jia Lingjiang River, Ming River, a small bay in a river River Drainage.This kind of meat quality of fish is fertile, is rich in fat.Growth is very fast, and individuality is also comparatively large, and generally can grow to 1-3 kilogram, maximum individuality can reach 10 kilograms.Abundance, is only second to neat mouth schizothoracin in the output in producing region.Be with in Yaan one, be referred to as " refined fish " with neat mouth schizothoracin, with neat mouth schizothoracin with the well-known four directions with " Yaan Fish Head en Casserole ".Except meat edible, though its ovum is poisonous, also edible after boiling.Be economic fish important in the water system of Upper Yangtze River each tributary, be also the middle-size and small-size aquifer cultivation industry of local development put object in a suitable place to breed.The cryopreservation method of fish sperm is mature on the whole, but species variation obviously exists, and does not have the report of this respect in the Semen routine field relating to Schizothorax davidi.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of Schizothorax davidi Semen routine method.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: provide a kind of Schizothorax davidi Semen routine method, comprise the following steps:
(1) semen collection: gather the good Schizothorax davidi seminal fluid of maturity, for subsequent use under depositing in 5 DEG C of clean environment;
(2) diluent preparing: preparation is containing the aqueous solution of weight ratio streptomycin 0.1%, NaCl 0.75%, KCl 0.32%, glucose 2.1%, sucrose 0.5%, sodium citrate 0.6%, sodium glutamate 0.8%, and adjust ph is 6.6;
(3) sperm dilution: by above-mentioned seminal fluid and dilution with after 1: 5 dilution ratio mixed diluting, adding antifreeze to final concentration is 5%, and balances 28 minutes under being put in 5 DEG C of conditions;
(4) semen freezing: the seminal fluid after above-mentioned dilution balance is placed in liquid nitrogen ullage 5 centimeters and balances 18 minutes, then above liquid nitrogen surface 1 centimeters to drop into Liquid nitrogen storage after balancing 8 minutes for subsequent use.
Carry out quality examination after described semen collection step: visually observing normal semen is milky, with simple microscope, under 10 × 40 times, with clear water activate, average motility rate is greater than 80% Semen routine for subsequent use, otherwise, abandon it.
Described antifreeze is the composition of volume ratio DMSO 20%, glycerine 25%, ethylene glycol 35%, DMA 25%.
The invention has the beneficial effects as follows:
(1) improve Schizothorax davidi mirror availability and outbreeding fertilization rate;
(2) conditional request of the present invention is low, and operation is easy to realize, and is suitable for large-scale cultivation and breeds.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
The embodiment of the present invention comprises:
Embodiment
A kind of Schizothorax davidi Semen routine method, comprises the following steps: (1) semen collection: gather the Schizothorax davidi seminal fluid that maturity is good, for subsequent use under depositing in 5 DEG C of clean environment;
(2) diluent preparing: preparation is containing the aqueous solution of weight ratio streptomycin 0.1%, NaCl 0.75%, KCl 0.32%, glucose 2.1%, sucrose 0.5%, sodium citrate 0.6%, sodium glutamate 0.8%, and adjust ph is 6.6;
(3) sperm dilution: by above-mentioned seminal fluid and dilution with after 1: 5 dilution ratio mixed diluting, adding antifreeze to final concentration is 5%, and balances 28 minutes under being put in 5 DEG C of conditions;
(4) semen freezing: the seminal fluid after above-mentioned dilution balance is placed in liquid nitrogen ullage 5 centimeters and balances 18 minutes, then above liquid nitrogen surface 1 centimeters to drop into Liquid nitrogen storage after balancing 8 minutes for subsequent use.
Carry out quality examination after described semen collection step: visually observing normal semen is milky, with simple microscope, under 10 × 40 times, with clear water activate, average motility rate is greater than 80% Semen routine for subsequent use, otherwise, abandon it.
Described antifreeze is the composition of volume ratio DMSO 20%, glycerine 25%, ethylene glycol 35%, DMA 25%.
The Schizothorax davidi seminal fluid fertilization utilizing the present invention to preserve, can reach 55%, higher than existing market about 22% at primitive gut fertilization rate in mid-term.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (3)
1. a Schizothorax davidi Semen routine method, is characterized in that, comprises the following steps:
(1) semen collection: gather the good Schizothorax davidi seminal fluid of maturity, for subsequent use under depositing in 5 DEG C of clean environment;
(2) diluent preparing: preparation is containing the aqueous solution of weight ratio streptomycin 0.1%, NaCl 0.75%, KCl 0.32%, glucose 2.1%, sucrose 0.5%, sodium citrate 0.6%, sodium glutamate 0.8%, and adjust ph is 6.6;
(3) sperm dilution: by above-mentioned seminal fluid and dilution with after 1: 5 dilution ratio mixed diluting, adding antifreeze to final concentration is 5%, and balances 28 minutes under being put in 5 DEG C of conditions;
(4) semen freezing: the seminal fluid after above-mentioned dilution balance is placed in liquid nitrogen ullage 5 centimeters and balances 18 minutes, then above liquid nitrogen surface 1 centimeters to drop into Liquid nitrogen storage after balancing 8 minutes for subsequent use.
2. Schizothorax davidi Semen routine method according to claim 1, is characterized in that:
Carry out quality examination after described semen collection step: visually observing normal semen is milky, with simple microscope, under 10 × 40 times, with clear water activate, average motility rate is greater than 80% Semen routine for subsequent use, otherwise, abandon it.
3. Schizothorax davidi Semen routine method according to claim 1 and 2, is characterized in that: described antifreeze is the composition of volume ratio DMSO 20%, glycerine 25%, ethylene glycol 35%, DMA 25%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510135862.XA CN104814005A (en) | 2015-03-26 | 2015-03-26 | Preservation method for seminal fluid of Schizothorax davidi |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510135862.XA CN104814005A (en) | 2015-03-26 | 2015-03-26 | Preservation method for seminal fluid of Schizothorax davidi |
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| CN104814005A true CN104814005A (en) | 2015-08-05 |
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| CN201510135862.XA Pending CN104814005A (en) | 2015-03-26 | 2015-03-26 | Preservation method for seminal fluid of Schizothorax davidi |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122668A (en) * | 2018-10-25 | 2019-01-04 | 镇江市绿色农业科技有限公司 | A kind of store method of hilsa herring sperm |
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| CN103518705A (en) * | 2013-10-17 | 2014-01-22 | 南京市水产科学研究所 | Yellow-head catfish meat semen preserving fluid and preparation method thereof |
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| CN101088511A (en) * | 2007-02-15 | 2007-12-19 | 中国水产科学研究院长江水产研究所 | Simple method for ultralow temperature preservation of fish sperm |
| CN101543209A (en) * | 2009-03-31 | 2009-09-30 | 华南农业大学 | Powder for chicken sperm dilution and preservation and preparation method and application thereof |
| CN101711522A (en) * | 2009-10-28 | 2010-05-26 | 广东海洋大学 | Ultralow-temperature preservation method of rachycentron canadum spermia |
| CN102077823A (en) * | 2010-11-16 | 2011-06-01 | 佛山市生生水产股份有限公司 | Method for cryopreservation of semen of siniperca scherzeri and artificial insemination |
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| CN103518705A (en) * | 2013-10-17 | 2014-01-22 | 南京市水产科学研究所 | Yellow-head catfish meat semen preserving fluid and preparation method thereof |
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| CN109122668A (en) * | 2018-10-25 | 2019-01-04 | 镇江市绿色农业科技有限公司 | A kind of store method of hilsa herring sperm |
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