CN110074283A - Improve compound formulation and the preparation of ocean bivalve shellfish maturity of fish gonads and immunity - Google Patents
Improve compound formulation and the preparation of ocean bivalve shellfish maturity of fish gonads and immunity Download PDFInfo
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- CN110074283A CN110074283A CN201910478699.5A CN201910478699A CN110074283A CN 110074283 A CN110074283 A CN 110074283A CN 201910478699 A CN201910478699 A CN 201910478699A CN 110074283 A CN110074283 A CN 110074283A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The present invention relates to compound formulation and the preparations of a kind of raising ocean bivalve shellfish maturity of fish gonads and immunity, belong to aquaculture feed additive field, the ingredient and mass fraction of the compound formulation are as follows: 18-20 parts of beta glucan, 3-5 parts of xylo-oligosaccharide, 18-22 parts of arginine, 40-50 parts of calcium chloride, 15-20 parts of magnesium sulfate, 5-7 parts of zinc sulfate, 40-45 parts of vitamin C, vitamin B14-5 parts, 26-30 parts of vitamin E, 4-5 parts of vitamin D, 4-5 parts of lecithin, split 4-5 parts of pot algae, 360-400 parts of EM bacterium, 600-640 parts of distilled water.Compound formulation formula of the present invention is reasonable as additive formula, synergistic, meets the gonad development demand of parent.Moreover, water quality improvement also is carried out with the use of EM bacterium, it is significant to optimize parent's fattening environment.Start in terms of body itself and breeding environment two, to the promotion significant effect of parent's maturity of fish gonads and immunity.
Description
Technical field:
The present invention relates to seawater bivalve shellfish seed breeding technical fields, more particularly to a kind of raising ocean bivalve shellfish
The compound formulation and preparation of parent's maturity of fish gonads and immunity.
Background technique:
China is marine shellfish cultivation big country, propagates type artificially already more than 40 kinds.Artificial seed's multiplication technique into
Step greatly supports, has pushed the healthy and sustainable development of marine shellfish aquaculture industry.2017, China's marine shellfish cultured output
Reach 1437.13 ten thousand tons, accounts for the 71.8% of sea-farming total output, and nursery amount reaches 2.48 trillion.Wherein, what master supported is male
The important bivalve seawater economic shellfish seed such as oyster, scallop, razor clam of hanging depends on artificial culture seed.
After decades of development, the full artificial breeding technology of most sea-farming bivalve shellfish seeds have built up and by
It walks perfect.But shellfish artificial seed breeding has always been considered as being high profit, high risk industry.The reason is that scale at present
Often there is serious " falling seedling " phenomenon in cultivating process, nursery is caused to be emerged there are still certain risk in full artificial seed's breeding
Rate is low, or even does not emerge, and causes serious financial consequences.
It is cultivated in work in ocean bivalve shellfish artificial seed, it is Seedling production that kind shellfish, which is temporarily supported, work of accelerating the ripening is particularly important
The key of work success or failure.It is obtained since filial generation early nutrition and immunity mainly pass through ovum from parent transmitting, so, plant shellfish
Sexual gland maturation quality directly affects filial generation hatching and late growth survival.But existing parent's accelerating approach is relatively fewer, mainly
There are two types of, i.e., nutrient accumulation is improved to increase effective accumulated temperature and the supply of enhancing bait by stepping up cultivation temperature.Moreover,
Due to bivalve shellfish filter-feeding feature, the main raising substance fed is unicellular alga, in addition to this, other relevant reports compared with
It is few.For the other kinds of aquiculture animals such as fish, shrimp crab, shellfish raising lacks mature man-made feeds, does not have more
There is the immune formulation of enhancing parent's maturity of fish gonads and immunity, this has seriously affected the high yield and stable yields of shellfish artificial seed,
The bottleneck to develop in a healthy way as the industry of restriction.
Summary of the invention:
The technical problem to be solved in the present invention is that providing a kind of raising ocean bivalve shellfish maturity of fish gonads and immunity
Compound formulation and preparation, fed during parent's accelerating by science, promote gonad development of parents and immune factor accumulation, into
And gamete maturation quality is improved, enhance parent and filial generation immunity of organisms, improves the productive and stability of artificial seed's production.
To achieve the above object, technical scheme is as follows:
A kind of compound formulation improving ocean bivalve shellfish maturity of fish gonads and immunity, component is according to quality proportioning
Are as follows: 18-20 parts of beta glucan, 3-5 parts of xylo-oligosaccharide, 18-22 parts of arginine, 40-50 parts of calcium chloride, 15-20 parts of magnesium sulfate, sulphur
Sour zinc 5-7 parts, 40-45 parts of vitamin C, vitamin B14-5 parts, 26-30 parts of vitamin E, 4-5 parts of vitamin D, lecithin
4-5 parts, split 4-5 parts of pot algae, 360-400 parts of EM bacterium, 600-640 parts of distilled water.
Preferably, the compound formulation component is according to quality proportioning are as follows: 20 parts of beta glucan, 4 parts of xylo-oligosaccharide, smart ammonia
20 parts of acid, 50 parts of calcium chloride, 18 parts of magnesium sulfate, 5 parts of zinc sulfate, 40 parts of vitamin C, vitamin B15 parts, vitamin E 30
Part, 5 parts of vitamin D, 5 parts of lecithin, split 5 parts of pot algae, 400 parts of EM bacterium, 600 parts of distilled water.
Preferably, the compound formulation component is according to quality proportioning are as follows: 18 parts of beta glucan, 5 parts of xylo-oligosaccharide, smart ammonia
18 parts of acid, 50 parts of calcium chloride, 20 parts of magnesium sulfate, 6 parts of zinc sulfate, 50 parts of vitamin C, vitamin B15 parts, vitamin E 30
Part, 5 parts of vitamin D, 5 parts of lecithin, split 5 parts of pot algae, 400 parts of EM bacterium, 600 parts of distilled water.
The preparation method of above-mentioned compound formulation includes: each group weighed in addition to EM bacterium and distilled water according to above-mentioned quality proportioning
Point, above-mentioned 600 parts of distilled water (referred to as solution A) is dissolved in after mixing.After each component is completely dissolved, protected with 500 mesh silk cover filterings
It is stored in glass container.
Further, above-mentioned 95% or more each component purity requirement.
Further, above-mentioned solution A and EM bacterium, when use, are ready-to-use.
Above-mentioned compound formulation is in the application for promoting maturity of fish gonads: the compound formulation is before use, by solution A and EM bacterium
It is mixed according to the ratio of 6:4；It hastens parturition 20-25 days a few days ago and starts in plan, after parent shellfish falls pond daily, compound formulation is uniformly splashed
In the water body for cultivating close shellfish, make final concentration of 3-4ppm of the compound formulation in water body, feeding compound formulation does not influence
Other all management works during incubation.
The present invention compared with prior art the utility model has the advantages that
1, compound formulation formula of the present invention includes immunopotentiator, incubation agent, vitamin addition as additive
Agent, trace mineral supplement, proportion is rationally, synergistic, meets the gonad development demand of parent.Moreover, being also used cooperatively EM
Bacterium carries out water quality improvement, significant to optimize parent's fattening environment.Start in terms of body itself and breeding environment two, to parent
The promotion significant effect of maturity of fish gonads and immunity.
2, compound formulation provided by the invention is used as additive, does not influence other any production journeys of parent's accelerating
Sequence.
3, raw material of the present invention is easy to get, and prepares simple and easy, and operability is stronger, is not required to particular facility, equipment, can be in shellfish
It is widely used in artificial seed's cultivating process.
Specific embodiment:
Technical solution of the present invention is further explained below by embodiment, but protection scope of the present invention not by
The limitation of embodiment in any form.
Embodiment 1
The present embodiment Chlamys farreri parent, is arranged 2 experimental groups, 1 group of test be by preferably compound formulation of the invention according to
Quality proportioning are as follows: 20 parts of beta glucan, 4 parts of xylo-oligosaccharide, 0 part of arginase 12,50 parts of calcium chloride, 18 parts of magnesium sulfate, zinc sulfate 5
Part, 40 parts of vitamin C, vitamin B15 parts, 30 parts of vitamin E, 5 parts of vitamin D, 5 parts of lecithin, 5 parts of pot algae are split, EM
400 parts of bacterium, 600 parts of distilled water.
Test 2 groups be by 20 parts of beta glucan, 4 parts of xylo-oligosaccharide, 0 part of arginase 12,50 parts of calcium chloride, 18 parts of magnesium sulfate,
5 parts of zinc sulfate, 40 parts of vitamin C, vitamin B15 parts, 30 parts of vitamin E, 5 parts of vitamin D, 400 parts of EM bacterium, distilled water
600 parts.
The preparation method of the compound formulation is as follows: electronic balance weighing beta glucan, xylo-oligosaccharide, arginine, chlorination
Calcium, magnesium sulfate, zinc sulfate, vitamin C, vitamin B1, vitamin E, vitamin D, (2 groups of test does not have lecithin with pot algae is split
Lecithin and split pot algae), its ratio be 20:4:20:50:18:5:40:5:30:5:5:5.After mixing, it is added and steams according to mass ratio
Distilled water 600 filters out impurity with 500 mesh thin,tough silk after completely dissolution, is stored in glassware (solution A).
The natural sea area Chlamys farreri mating period previous moon sea area 2 age scallop is moved into workshop.It is counted according to work
It draws, in hastening parturition, first 20 days start to feed compound formulation.
The compound formulation is above-mentioned solution A and EM bacterium with 6:4 proportioning concentration.
Described to feed operation after parent falls pond daily, feeding dosage is 4ppm.
According to above scheme, hasten parturition after continuously feeding 20 days, statistical analysis control group and the oviposition for feeding experimental group parent
Rate and sperm drainage rate, three kinds of superoxide dismutase, lysozyme and catalase etc. immune related enzyme activities and hatching rate with
Filial generation larva growing state.The result shows that the spawning rate and sperm drainage rate of 1 group of parent shellfish of test are respectively 81.35 ± 3.69% Hes
79.63 ± 4.90%, the spawning rate and sperm drainage rate for testing 2 groups of parent shellfishes are respectively 77.64 ± 3.27 and 74.25 ± 3.52 control groups
14.64% He is respectively increased than control group in respectively 70.96 ± 2.13% and 66.91 ± 5.93% (tables 1), 1 group of experiment
19.01%.It tests 1 group and is respectively increased 4.78% and 7.25% than 2 groups of experiment, enzyme activity determination the results are shown in Table 2, test 1 group of property
Three kinds of enzyme activities of glandular tissue and musculature are all obviously improved compared with the control group, superoxide dismutase, the lysozyme of sexual gland
Enzyme activity with catalase is 2.56 times, 2.88 times and 2.58 times of control group respectively, musculature is 1.54 times respectively,
1.29 times and 2.45 times.Testing 1 group and control group group the incubation rate of the larvaes is respectively 92.83 ± 1.58% and 90.13 ± 2.78%
(table 1) improves 2.7 percentage points；The shell length of growth aspect, 3 day old larvas has essentially no difference, 6 ages in days surveyed, 9 days
Age, 12 ages in days, 15 ages in days and 22 instar larvae shells are long, and 1 group of experiment has a clear superiority (table 3).
It tests three kinds of enzyme activities of 2 groups of gonadal tissues and musculature to be obviously improved compared with the control group, sexual gland surpasses
The enzyme activity of superoxide dismutase, lysozyme and catalase is 1.69 times, 1.59 times and 2.01 times of control group, flesh respectively
Meat tissue is 1.19 times, 1.11 times and 1.73 times (table 2) respectively.Testing 1 group and control group group the incubation rate of the larvaes is respectively 92.16
± 2.51% and 90.13 ± 2.78% (table 1), improve 2.03 percentage points；2 groups are tested in terms of growth, wherein 6 ages in days, 9
Age in days, 12 ages in days, 15 ages in days and 22 instar larvae shells length are superior to control group (table 3).
1 group is tested compared with 2 groups of experiment, in terms of three kinds of enzyme activities of gonadal tissue and musculature, tests 1 group than experiment
The enzyme activity of the superoxide dismutases of 2 groups of sexual glands, lysozyme and catalase has been respectively increased 51.39%, 80.17%,
28.54%, the enzyme activity in musculature has been respectively increased 28.77%, 15.79%, 41.39%.Test 1 group and 2 groups of experiment
The incubation rate of the larvaes does not have significant difference.In terms of larva growth, the larval growth rates of 1 group of experiment are better than 2 groups of experiment, 22
The most obvious in day old larva, the embryophores on the 22nd that 1 group 2 groups than experiment of experiment are long to be higher by 3.05%.
To sum up, 1 group of parental performance is tested to go out than control group and 2 groups of experiment with higher spawning rate and sperm drainage rate, simultaneously
Immune correlation enzyme activity also significantly improves, and 1 group of experiment has a clear superiority in terms of the speed of growth.This shows to utilize this compound system
Agent can effectively improve ocean bivalve shellfish parent maturity of fish gonads and immunity.
Table 1 feeds group and control group parent spawning rate, sperm drainage rate and the incubation rate of the larvaes comparison
Unit: (%)
Table 2 feeds group and the immune related enzyme activity comparison of control group parent's shellfish different tissues
Unit: (U/mgprot)
Table 3 feeds group and the long comparison of control group larvae development shell early period
Unit: μm
Claims (4)
1. a kind of compound formulation for improving ocean bivalve shellfish maturity of fish gonads and immunity, it is characterised in that the compound formulation
Ingredient and mass fraction are as follows: 18-20 parts of beta glucan, 3-5 parts of xylo-oligosaccharide, 18-22 parts of arginine, 40-50 parts of calcium chloride,
15-20 parts of magnesium sulfate, 5-7 parts of zinc sulfate, 40-45 parts of vitamin C, vitamin B14-5 parts, 6-30 parts of vitamin E2, vitamin
D4-5 parts, 4-5 parts of lecithin, split 4-5 parts of pot algae, 360-400 parts of EM bacterium, 600-640 parts of distilled water.
2. a kind of compound formulation for improving ocean bivalve shellfish maturity of fish gonads and immunity according to claim 1,
It is characterized in that the ingredient and mass fraction of the compound formulation are as follows: 20 parts of beta glucan, 4 parts of xylo-oligosaccharide, 0 part of arginase 12, chlorine
50 parts of calcium of change, 18 parts of magnesium sulfate, 5 parts of zinc sulfate, 40 parts of vitamin C, vitamin B15 parts, 30 parts of vitamin E, Vitamin D5
Part, 5 parts of lecithin, split 5 parts of pot algae, 400 parts of EM bacterium, 600 parts of distilled water.
3. a kind of compound formulation for improving ocean bivalve shellfish maturity of fish gonads and immunity according to claim 1,
It is characterized in that the compound formulation component according to quality proportioning are as follows: 18 parts of beta glucan, 5 parts of xylo-oligosaccharide, 18 parts of arginine, chlorine
50 parts of calcium of change, 20 parts of magnesium sulfate, 6 parts of zinc sulfate, 50 parts of vitamin C, vitamin B15 parts, 30 parts of vitamin E, Vitamin D5
Part, 5 parts of lecithin, split 5 parts of pot algae, 400 parts of EM bacterium, 600 parts of distilled water.
4. the preparation method of any one of the claim 1-3 compound formulation includes: to weigh according to above-mentioned quality proportioning except EM
Each component outside bacterium and distilled water is dissolved in above-mentioned distilled water, referred to as solution A after mixing；After each component is completely dissolved, use
500 mesh silk cover filterings are stored in glass container；Solution A and EM bacterium, when use, are ready-to-use.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158659A (en) * | 2021-12-17 | 2022-03-11 | 江苏省农业科学院宿迁农科所 | Composite preparation for improving immunity of juvenile procambarus clarkia and using method thereof |
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2019
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158659A (en) * | 2021-12-17 | 2022-03-11 | 江苏省农业科学院宿迁农科所 | Composite preparation for improving immunity of juvenile procambarus clarkia and using method thereof |
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