CN108576495A - A kind of breeding additive of Litopenaeus vannamei Broodstock and its application - Google Patents
A kind of breeding additive of Litopenaeus vannamei Broodstock and its application Download PDFInfo
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- CN108576495A CN108576495A CN201810401551.7A CN201810401551A CN108576495A CN 108576495 A CN108576495 A CN 108576495A CN 201810401551 A CN201810401551 A CN 201810401551A CN 108576495 A CN108576495 A CN 108576495A
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- breeding
- additive
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- protease
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/168—Steroids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
Abstract
The present invention provides a kind of to improve breeding additive and its application of the nutrition supply of close shrimp with the Litopenaeus vannamei Broodstock of the property demand in breeding period, using glucan, Coating sodium butyrate, nucleotide, antibacterial peptide, bacillus isoreactivity ingredient, improve the intestinal health and anti-stress ability of prawn, the vigor and mating rate of prawn are improved, the drugs frequency of use such as disinfection class and management are reduced；Emulsifier, protease is used to be digested and assimilated to high-protein high-fat feed to improve liver sausage, drastically demand of the close shrimp to high nutrition ingredient during meeting breeding；Using ingredients such as yeast, spirulina powder and carrotene, the related pigment composition being rich in using it, abundant cellular content improve the development quality of close shrimp hepatopancrease and sexual gland；Shorten sexual gland maturation time with mating chemical compositions, raising sexual gland quality such as vitamin C, vitamin E, cholesterol, improves the hatching rate of fertilized eggs and the survival rate of the young.
Description
Technical field
The present invention relates to aquaculture feed and raising technology, the breeding additive of especially a kind of Litopenaeus vannamei Broodstock
And its application.
Background technology
Litopenaeus vannamei（Litopenaeus vannamei）, it is commonly called as Penaeus Vannmei, originates in the eurysalinity first in America
Shell class, the kind is big with yield, dressing percentage is high, the features such as being easy to intensive culture and delicious meat and rapidly become aquatic products and support
The upstart of industry is grown, in the latest 20 years, China's litopenaeus vannamei cultured output occupies the first in the world, develops into blue agriculture warp
The important force of Ji industry.But China is mainly by from the national import parent shrimp such as U.S., not only of high cost, the seedling quality of cultivation
Also irregular, cause very big cultivation risk.How to cultivate health young shrimp becomes the key restriction factors for solving prawn culturing.
The nutrition supply cultivated during depending on close shrimp breeding and health control level of health young shrimp, this is to determine close shrimp
The critical stage of gonad development, Fertilized quality and hatching rate and young survival rate.Parent shrimp breeds generally existing sexual gland at present
Maturity deficiency, mating rate deficiency, Egg-laying interval renewal, fish brood amount is insufficient, is even drawn due to Fertilized quality problem
The defects of hatching rate deficiency risen is insufficient with young survival rate.Through investigation and analysis, at present the close shrimp of breeding mainly use squid, clam worm,
The fresh foods such as oyster are directly fed, although these biological feeds are full of nutrition, there is also unbalanced problem, and also it is interim
Suitability is insufficient.It can be seen that the common shrimp material used in the cultivation of market cannot meet the nutritional need in parent shrimp rearing stage.
Invention content
The object of the present invention is to provide a kind of breeding additives of Litopenaeus vannamei Broodstock, to improve the nutrition supply of close shrimp
With the property demand in breeding period.The present invention is using glucan, Coating sodium butyrate, nucleotide, antibacterial peptide, bacillus etc.
Active constituent improves the intestinal health and anti-stress ability of prawn, improves the vigor and mating rate of prawn, reduces the medicines such as disinfection class
Object frequency of use and management；It uses emulsifier, protease to be digested and assimilated to high-protein high-fat feed to improve liver sausage, meets
Drastically demand of the close shrimp to high nutrition ingredient during breeding；Using ingredients such as yeast, spirulina powder and carrotene, its richness is utilized
The related pigment composition, the abundant cellular content that contain improve the development quality of close shrimp hepatopancrease and sexual gland.Inventor is by wound
Make sex exploration, the mating chemical compositions such as the above ingredient and vitamin C, vitamin E, cholesterol, to improve sexual gland quality, shorten sexual gland at
The ripe time improves the hatching rate of fertilized eggs, and the survival rate effect for improving the young is more notable.
The present invention also aims to provide the application of the breeding additive of the Litopenaeus vannamei Broodstock.
A kind of breeding additive of Litopenaeus vannamei Broodstock of the present invention, parts by weight composition are as follows：
Ocean rhodotorula 5-15；Vitamin C ester 5-10；Vitamin E 2-5；Beta carotene 1-5；Cholesterol 3-8；It is anti-
Bacterium peptide 1-5；β-1,3 glucans 5-15；Digest beer yeast powder 5-15；Spirulina powder 8-20；Emulsifier 1-5；Egg
White enzyme 1-5；Glycine zine 0.5-3；L- selenomethionines 0.2-1；Coating sodium butyrate 2-6；Bacillus licheniformis 5-
10；Kelp Powder 10-30.
The preferred embodiment of the present invention is as follows：Ocean rhodotorula 15, vitamin C ester 8, vitamin E 3.5；Beta carotene
2.5, cholesterol 3, antibacterial peptide 5, emulsifier 3, protease 2, glycine zine 1.2；L- selenomethionines 0.3；Coating butyric acid
Sodium 3, β -1,3 Dextran 8s, enzymolysis beer yeast powder 11, spirulina powder 9, bacillus licheniformis 8, Kelp Powder, 17.5.
Inventor obtains that preferred embodiment is one of following or combination of the above by creating sex exploration：
The mixture of the preferred polyethylene glycol glycerol ricinoleic acid fat of emulsifier, glycerin monostearate, lauric acid fat.
The compound alkali protease of serine class that protease is preferably generated with fermentation of bacillus subtilis.
The cecropin antimicrobial peptides that the preferred fermentation of bacillus subtilis of antibacterial peptide generates.
The application of the breeding additive of Litopenaeus vannamei Broodstock of the present invention, is to add the breeding of Litopenaeus vannamei Broodstock
Add agent to wear into fine powder, crosses 100 mesh, mixing, the coefficient of variation≤5%, according to the addition of 1% weight or in ice in artifical compound feed
According to 5% weight ratio spice in fresh bait material, feed.
Compared with prior art, the present invention having the following advantages that：
（1）Shorten the sexual gland processing maturation period during close shrimp breeding, improves the ripe ratio and mating rate of sexual gland；
（2）The quality and hatching rate for improving fertilized eggs, improve the survival rate of the young；
（3）Promote close shrimp intestinal health and digest and assimilate, improves immunity.
（4）It is easy to use, can both be added to mixed feed, also can directly spice fresh food feed.
Specific implementation mode
Embodiment 1
The breeding additive formulations of Litopenaeus vannamei Broodstock are as follows：Ocean rhodotorula 15kg, vitamin C ester 8kg, vitamin E
3.5kg；Beta carotene 2.5kg, cholesterol 3kg, antibacterial peptide 5kg, emulsifier 3kg, protease 2kg, glycine zine
1.2kg；L- selenomethionines 0.3kg；The Coating sodium butyrate Dextran 8 kg of 3kg, β -1,3 digests beer yeast powder 11kg,
Spirulina powder 9kg, bacillus licheniformis 8kg, Kelp Powder, 17.5kg.
The above ingredient is worn into fine powder, crosses 100 mesh, mixing, the coefficient of variation≤5%, according to 1% weight in artifical compound feed
Amount is added or in chilled bait according to 5% weight ratio spice, is fed.
Embodiment 2
The breeding additive formulations of Litopenaeus vannamei Broodstock are as follows：5 kg of ocean rhodotorula；10 kg of vitamin C ester；Vitamin E
2 kg；5 kg of beta carotene；8 kg of cholesterol；1 kg of antibacterial peptide；β -1,3 Dextran 15s kg；Digest beer yeast powder 5
kg；20 kg of spirulina powder；1 kg of emulsifier；5 kg of protease；3 kg of glycine zine；0.2 kg of L- selenomethionines；Coating
6 kg of sodium butyrate；5 kg of bacillus licheniformis；30 kg of Kelp Powder.
The above ingredient is worn into fine powder, crosses 100 mesh, mixing, the coefficient of variation≤5%, according to 1% weight in artifical compound feed
Amount is added or in chilled bait according to 5% weight ratio spice, is fed.
Embodiment 3
The breeding additive formulations of Litopenaeus vannamei Broodstock are as follows：10 kg of ocean rhodotorula；5 kg of vitamin C ester；Vitamin E
5 kg；1 kg of beta carotene；5 kg of cholesterol；3 kg of antibacterial peptide；5 kg of β -1,3 glucans；Digest beer yeast powder 15
kg；8 kg of spirulina powder；5 kg protease of emulsifier, 1 kg；0.5 kg of glycine zine；1 kg of L- selenomethionines；Coating fourth
2 kg of sour sodium；10 kg of bacillus licheniformis；10 kg of Kelp Powder.
The above ingredient is worn into fine powder, crosses 100 mesh, mixing, the coefficient of variation≤5%, according to 1% weight in artifical compound feed
Amount is added or in chilled bait according to 5% weight ratio spice, is fed.
Application effect is tested
Select just primary Litopenaeus vannamei Broodstock of laying eggs（Weight 50g or so）, 10 tails of density/cubic meter, with 3 kinds of differences
Bait feeding, 3 repetitions of each bait.With commercial ice fresh bait material（Clam worm：Squid=3:1）Bait as a comparison case, with implementation
Example 1,2 compares, and obtains data such as following table：
Note：Different letters indicate that otherness is notable in shoulder mark
Examples 1 and 2 and comparative example data analysis：Although the indexs such as genital gland indices and mating rate do not have significant difference,
Sexal maturity time interval, egg laying amount and hatching rate etc. performance are apparent, illustrate the synergist of the addition present invention to the numerous of close shrimp
Fertility can be apparent；Embodiment 1 is close with 2 effect, shows for additive capacity and type, the present invention is rationally effective.
In conclusion the close shrimp of the present invention breeds Synergistic additives, the special dietary demand in close shrimp breeding stage can be met,
The cycle of gonadal development for shortening close shrimp improves the ripe ratio and mating rate of close shrimp sexual gland, improves quality and the hatching of fertilized eggs
Rate improves young survival rate.
Claims (6)
1. a kind of breeding additive of Litopenaeus vannamei Broodstock, it is characterised in that its parts by weight composition is as follows：Ocean rhodotorula
5—15；Vitamin C ester 5-10；Vitamin E 2-5；Beta carotene 1-5；Cholesterol 3-8；Antibacterial peptide 1-5；β-1,
3 glucans 5-15；Digest beer yeast powder 5-15；Spirulina powder 8-20；Emulsifier 1-5；Protease 1-5；Sweet ammonia
Sour zinc 0.5-3；L- selenomethionines 0.2-1；Coating sodium butyrate 2-6；Bacillus licheniformis 5-10；Kelp Powder 10-30.
2. additive according to claim 1, it is characterised in that its parts by weight composition is as follows：Ocean rhodotorula 15, dimension
Raw element C esters 8, vitamin E 3.5；Beta carotene 2.5, cholesterol 3, antibacterial peptide 5, emulsifier 3, protease 2, glycine
Zinc 1.2；L- selenomethionines 0.3；Coating sodium butyrate 3, β -1,3 Dextran 8s digest beer yeast powder 11, spirulina powder
9, bacillus licheniformis 8, Kelp Powder, 17.5.
3. additive according to claim 1 or 2, it is characterised in that emulsifier be selected from polyethylene glycol glycerol ricinoleic acid fat,
The mixture of glycerin monostearate, lauric acid fat.
4. additive according to claim 3, it is characterised in that protease is selected from and is generated with fermentation of bacillus subtilis
The compound alkali protease of serine class.
5. additive according to claim 4, it is characterised in that antibacterial peptide is selected from the day that fermentation of bacillus subtilis generates
Sbombycin antibacterial peptide.
6. the application of the breeding additive of one of claim 1-5 Litopenaeus vannamei Broodstock, it is characterised in that by vannamei boone
The breeding additive of prawn wears into fine powder, crosses 100 mesh, mixing, the coefficient of variation≤5%, according to 1% in artifical compound feed
Weight is added or in chilled bait according to 5% weight ratio spice, is fed.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2018103508930 | 2018-04-18 | ||
| CN201810350893 | 2018-04-18 |
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| Publication Number | Publication Date |
|---|---|
| CN108576495A true CN108576495A (en) | 2018-09-28 |
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| CN201810401551.7A Pending CN108576495A (en) | 2018-04-18 | 2018-04-28 | A kind of breeding additive of Litopenaeus vannamei Broodstock and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115736131A (en) * | 2022-10-27 | 2023-03-07 | 广东海大集团股份有限公司 | Penaeus vannamei parent feed additive and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1375215A (en) * | 2002-04-12 | 2002-10-23 | 中国科学院南海海洋研究所 | Granular shrimp feed |
| CN1620895A (en) * | 2004-12-10 | 2005-06-01 | 中国科学院南海海洋研究所 | Nutrient reinforcer for shrimp |
| CN1739377A (en) * | 2005-09-13 | 2006-03-01 | 中国水产科学研究院南海水产研究所 | Sex gland mature promoting feed additive for tigar prawn |
| KR20120081841A (en) * | 2011-01-12 | 2012-07-20 | 강원대학교산학협력단 | Feed composition for p.vannamei |
| KR20140106116A (en) * | 2013-02-26 | 2014-09-03 | 한남바이오 주식회사 | Feed composition for Crustacean |
-
2018
- 2018-04-28 CN CN201810401551.7A patent/CN108576495A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1375215A (en) * | 2002-04-12 | 2002-10-23 | 中国科学院南海海洋研究所 | Granular shrimp feed |
| CN1620895A (en) * | 2004-12-10 | 2005-06-01 | 中国科学院南海海洋研究所 | Nutrient reinforcer for shrimp |
| CN1739377A (en) * | 2005-09-13 | 2006-03-01 | 中国水产科学研究院南海水产研究所 | Sex gland mature promoting feed additive for tigar prawn |
| KR20120081841A (en) * | 2011-01-12 | 2012-07-20 | 강원대학교산학협력단 | Feed composition for p.vannamei |
| KR20140106116A (en) * | 2013-02-26 | 2014-09-03 | 한남바이오 주식회사 | Feed composition for Crustacean |
Non-Patent Citations (4)
| Title |
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| 杜少波等: "亲虾营养需求研究进展 ", 《热带海洋学报》 * |
| 欧阳冰等: "抗菌肽新型预混合剂饲养南美白对虾的效果试验 ", 《广东饲料》 * |
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| 艾春香: "功能性水产配合饲料的研发与应用", 《饲料工业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115736131A (en) * | 2022-10-27 | 2023-03-07 | 广东海大集团股份有限公司 | Penaeus vannamei parent feed additive and preparation method and application thereof |
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