CN110313561A - A kind of functional feed for coping with Fugu rubripes winter syndrome - Google Patents

A kind of functional feed for coping with Fugu rubripes winter syndrome Download PDF

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Publication number
CN110313561A
CN110313561A CN201910724740.2A CN201910724740A CN110313561A CN 110313561 A CN110313561 A CN 110313561A CN 201910724740 A CN201910724740 A CN 201910724740A CN 110313561 A CN110313561 A CN 110313561A
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feed
vitamin
fish
fugu rubripes
composite
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卫育良
梁萌青
徐后国
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The present invention discloses a kind of functional feed for coping with Fugu rubripes winter syndrome, belong to aquatic feeds field, the functional feed is on the basis of existing composite fish bait for red-fin Eastern-Globefish, with fish meal and liquid fish substitution part Gluten, corn protein powder and the concentrated albumen-powder of soybeans, reducing the protein content in Fugu rubripes feed by 2 percentage points is 46%；The vegetable oil usage amount in formula is reduced simultaneously, and reducing the fat content in Fugu rubripes feed by 2 percentage points is 9%.Functional feed of the invention compares iced trash fish, under conditions of Fugu rubripes grows without significant difference, improves liver oxidation resistance, significantly reduces 19% winter syndrome disease incidence, improves survival rate.Meanwhile this feed formula also as save forage protein and fatty resource, reduces aquaculture cost, it is ensured that the yield and benefit that the overwintering seed of Fugu rubripes is formed.

Description

A kind of functional feed for coping with Fugu rubripes winter syndrome
Technical field
The invention belongs to seawater fish mixed feed technical fields, and in particular to a kind of reply Fugu rubripes winter is comprehensive The functional feed of sign.
Background technique
Fugu rubripes (Takifugu rubripes) is commonly called as river Puffer, be the important sea-farming kind of northern China it One, economic value with higher, at present to export based on Japan and Korea S, commercial specification is in 600g or more.Fugu rubripes cultivation It is suitable for water temperature at 16-23 DEG C, does not ingest substantially at 10 DEG C or less, and northern China starts in late October or early November to the In April, 2, ocean temperature are not suitable for cultivation.Thus, it is ensured that Fugu rubripes successfully cultivates in the north, and meet market Demand to big specification Fugu rubripes just must pass through the cultivation of outdoor ponds seed or marine net cage in the seed of current year hatching After seed cultivation, it is transferred to the overwintering cultivation of indoor carry out seed.
The overwintering cultivation of Fugu rubripes seed is the basis for obtaining sufficient high-quality healthy seedling, and next year adult fish is supported The guarantee grown.However, different from the northern sea-farming kind such as turbot, overwintering cultivation can be because being up to indoors for Fugu rubripes The cold temperature culture environment of several months leads to reduction of ingesting first, and then generates physical stress, metabolic inhibition, immunity degradation, cause of disease The a series of symptoms such as microorganism infection, it is final that the winters syndromes such as fish body vigor declines, liver whitens, the death rate increases occur. Therefore, the functional feed of exploitation reply Fugu rubripes winter syndrome improves overwintering red fin east by nutrition regulation technology Puffer seed ingests, and enhances the resistance of fish body, is of great significance to Fugu rubripes winter syndrome is solved, is also to ensure that The key link of Fugu rubripes yield and benefit.
Summary of the invention
The technical problem to be solved in the present invention is to provide it is a kind of cope with Fugu rubripes winter syndrome functional feed, with It solves to cause the overwintering immunity degradation of seed after it generates winter syndrome in During Overwintering Period, the problem of survival rate reduces.
The present invention is completed by following operating technology:
A kind of functional feed for coping with Fugu rubripes winter syndrome, the functional collaboration feed is in existing red fin On the basis of Fugu mixed feed, it is concentrated with fish meal and liquid fish substitution part Gluten, corn protein powder and soybean Albumen powder, reducing the protein content in Fugu rubripes feed by 2 percentage points is 46%；The vegetable oil in formula is reduced simultaneously Usage amount, reducing the fat content in Fugu rubripes feed by 2 percentage points is 9%.
Further, add a kind of composite feeding promoting agent in the functional feed, the composite feeding promoting agent include alanine, Glycine and DMPT (sulfobetaine), the mass ratio of 3 kinds of ingredients are 1:1:2, matter of the composite feeding promoting agent in feed Measuring score is 0.5%.
Further, a kind of composite functional additive, the composite functional additive are added in the functional feed Including vitamin C, vitamin E, inositol, taurine and Na2SeO3, the mass ratio of 5 kinds of ingredients is 50:30:2:60:5, described Mass fraction of the composite functional additive in feed is 1.62%.
Further, the ingredient of the functional feed and mass fraction are respectively as follows: white fish meal, and 46%；Liquid fish, 5%； Brewer's yeast, 3%；Gluten, 2%；Corn protein powder, 1%；Soy protein concentrate, 1%；Wheat flour, 31.18%；Fish oil, 2.7%；Phosphatide, 1.5%；Calcium dihydrogen phosphate, 1.5%；Vitamin mixtures, 1%；Mineral mixture, 1%；Compound food calling Agent, 0.5%；Composite functional additive, 1.62%.
The composite feeding promoting agent is alanine, glycine and DMPT, and the mass ratio of 3 kinds of ingredients is 1:1:2.
The composite functional additive is vitamin C, vitamin E, inositol, taurine and Na2SeO3, 5 kinds of ingredients Mass ratio is 50:30:2:60:5.
Vitamin mixtures (the mgkg-1Feed) it include: thiamine, 25mg；Riboflavin, 45mg；Puridoxine hydrochloride, 20mg；Vitamin B12, 0.1mg；Vitamin K3, 10mg；Inositol, 800mg；Pantothenic acid, 60mg；Niacin, 200mg；Folic acid, 20mg； Biotin, 1.20mg；Vitamin A, 32mg；Vitamin D, 5mg；Vitamin E, 120mg；Wheat-middlings 8.67g.
Mineral element mixture (the mgkg-1Feed) it include: sodium fluoride, 2mg；Potassium iodide, 0.8mg；Cobalt chloride, 50mg；Copper sulphate, 10mg；Ferric sulfate, 80mg；Zinc sulfate, 50mg；Magnesium sulfate, 1200mg；Calcium dihydrogen phosphate, 3000mg；Chlorination Sodium, 100mg；Zeolite powder, 5.51g.
The present invention compared with prior art the utility model has the advantages that
(1) present invention uses fish meal and liquid fish, reduces existing composite fish bait for red-fin Eastern-Globefish crude protein content 2%, make the protein content 46% in Fugu rubripes functional feed, improves Fugu rubripes and the digestion of protein is inhaled It produces effects rate, saves forage protein, reduce nitrogen discharge and phosphorus discharge.
(2) present invention reduces existing composite fish bait for red-fin Eastern-Globefish fat content 2%, makes Fugu rubripes functional feed In fat content be 9%, reduce winter low temperature environment Fugu rubripes endogenous fat to liver rouge caused by hepatic transport Fat deposition and denaturation, improve the function of liver.
(3) present invention addition composite feeding promoting agent, improves ingesting for Fugu rubripes, slows down fish reduction under cryogenic conditions and takes the photograph Appetite is hoped, to reduce the winter syndrome disease incidence of Fugu rubripes.
(4) present invention addition composite functional additive, improves the oxidation resistance of Fugu rubripes body, enhances fish body Immunity, to improve the resistance to low temperature environment.Compared with existing commercial feed and iced trash fish, feed of the present invention drops respectively The disease incidence of low Fugu rubripes winter syndrome 28% and 19%.
Detailed description of the invention
Fig. 1 Fugu rubripes suffers from winter syndrome judgment basis: there is hyperemia, partially in faint yellow or white, empty in liver 4 kinds of symptoms of such as A, B, C and the D such as alveolation, are determined as illness；Liver is pink and the state without vacuolation such as E, is judged to being good for Health.
Specific embodiment
Below by specific embodiment, to further illustrate the technical scheme of the present invention, but protection scope of the present invention are not It is limited in any form by embodiment.
Embodiment 1
This control provides the overwintering control group feed of Fugu rubripes, composite fish bait for red-fin Eastern-Globefish formula described in example It is made of the raw material of following mass fraction: white fish meal, 40%；Brewer's yeast, 3%；Gluten, 6%；Corn protein powder, 6%； Soy protein concentrate, 6%；Wheat flour, 27.9%；Fish oil, 2.7%；Soya-bean oil, 2.4%；Phosphatide, 1.5%；Calcium dihydrogen phosphate, 1.5%；Vitamin mixtures, 1%；Mineral mixture, 1%.
Vitamin mixtures (the mgkg-1Feed) it include: thiamine, 25mg；Riboflavin, 45mg；Puridoxine hydrochloride, 20mg；Vitamin B12, 0.1mg；Vitamin K3, 10mg；Inositol, 800mg；Pantothenic acid, 60mg；Niacin, 200mg；Folic acid, 20mg； Biotin, 1.20mg；Vitamin A, 32mg；Vitamin D, 5mg；Vitamin E, 120mg；Wheat-middlings 8.67g.
Mineral element mixture (the mgkg-1Feed) it include: sodium fluoride, 2mg；Potassium iodide, 0.8mg；Cobalt chloride, 50mg；Copper sulphate, 10mg；Ferric sulfate, 80mg；Zinc sulfate, 50mg；Magnesium sulfate, 1200mg；Calcium dihydrogen phosphate, 3000mg；Chlorination Sodium, 100mg；Zeolite powder, 5.51g.
The protein content that the composite fish bait for red-fin Eastern-Globefish measures is 48%, fat content 11%.
Embodiment 2
The present embodiment provides a kind of a kind of functional feeds for reducing albumen and fat content, by additionally increasing fish meal and water Part Gluten, corn protein powder and the concentrated albumen-powder of soybeans in Fish protein substitution control group feed are solved, to reduce in feed Protein content, reduce fat content by directly reducing in feed plant source fat additive amount.Composite fish bait for red-fin Eastern-Globefish Formula is made of the raw material of following mass fraction: white fish meal, and 46%；Liquid fish, 5%；Brewer's yeast, 3%；Gluten, 2%；Corn protein powder, 1%；Soy protein concentrate, 1%；Wheat flour, 33.3%；Fish oil, 2.7%；Phosphatide, 1.5%；Phosphoric acid Calcium dihydrogen, 1.5%；Vitamin mixtures, 1%；Mineral mixture, 1%.
Vitamin mixtures (the mgkg-1Feed) it include: thiamine, 25mg；Riboflavin, 45mg；Puridoxine hydrochloride, 20mg；Vitamin B12, 0.1mg；Vitamin K3, 10mg；Inositol, 800mg；Pantothenic acid, 60mg；Niacin, 200mg；Folic acid, 20mg； Biotin, 1.20mg；Vitamin A, 32mg；Vitamin D, 5mg；Vitamin E, 120mg；Wheat-middlings 8.67g.
Mineral element mixture (the mgkg-1Feed) it include: sodium fluoride, 2mg；Potassium iodide, 0.8mg；Cobalt chloride, 50mg；Copper sulphate, 10mg；Ferric sulfate, 80mg；Zinc sulfate, 50mg；Magnesium sulfate, 1200mg；Calcium dihydrogen phosphate, 3000mg；Chlorination Sodium, 100mg；Zeolite powder, 5.51g.
The crude protein content that the composite fish bait for red-fin Eastern-Globefish measures is 46%, crude fat content 9%.
Using 2 mixed feed of above-described embodiment 1 and embodiment, the Fugu rubripes juvenile fish of original body mass 150g is selected, 15 DEG C of water temperature of room flowing water culture system carries out 10 weeks by a definite date culture experiments, and every group sets 3 repetitions, after experiment, measurement The indexs such as survival, growth, liver index, the internal organ index of fish.Experimental result is shown in Tables 1 and 2s.
1. Examples 1 and 2 of table test fish growth result
The experiment ichthyoid index of 2. Examples 1 and 2 of table
Liver body ratio (%) Dirty body ratio (%) Coefficient of condition (g/cm3)
Embodiment 1 8.61±1.54b 13.76±0.46b 3.72±0.10
Embodiment 2 7.39±1.69a 11.68±0.67a 3.91±0.18
T is examined
P value 0.033 0.049 0.368
By the result of Tables 1 and 2 it is found that although the growth result of embodiment 1 and embodiment 2 and control group statistically Without significant difference, but therefore 2 groups of the embodiment liver body ratios and dirty body ratio for reducing Fugu rubripes compare control in embodiment 2 Under conditions of group reduces forage protein and fat content, not only guarantee to grow unaffected, but also reduces liver and interior fat is heavy Product, ensure that the health of fish body.In addition, the program also as save the albumen and fat of feed, reduce to possible in water body Nitrogen discharge and phosphorus discharge.In conclusion embodiment 2 reduces forage protein and the embodiment of fat content is feasible and effective.
Embodiment 3
The present embodiment provides composite fish bait for red-fin Eastern-Globefish composite feeding promoting agent, Fugu rubripes cooperation as described in the examples Feed formula is made of the raw material of following mass fraction: white fish meal, and 46%；Liquid fish, 5%；Brewer's yeast, 3%；Glutelin Powder, 2%；Corn protein powder, 1%；Soy protein concentrate, 1%；Wheat flour, 32.8%；Fish oil, 2.7%；Phosphatide, 1.5%；Phosphorus Acid dihydride calcium, 1.5%；Vitamin mixtures, 1%；Mineral mixture, 1%；Composite feeding promoting agent, 0.5%.
The composite feeding promoting agent is alanine, glycine and DMPT, and mass fraction of each ingredient in feed is respectively 0.125%, 0.125%, 0.25%.
Vitamin mixtures (the mgkg-1Feed) it include: thiamine, 25mg；Riboflavin, 45mg；Puridoxine hydrochloride, 20mg；Vitamin B12, 0.1mg；Vitamin K3, 10mg；Inositol, 800mg；Pantothenic acid, 60mg；Niacin, 200mg；Folic acid, 20mg； Biotin, 1.20mg；Vitamin A, 32mg；Vitamin D, 5mg；Vitamin E, 120mg；Wheat-middlings 8.67g.
Mineral element mixture (the mgkg-1Feed) it include: sodium fluoride, 2mg；Potassium iodide, 0.8mg；Cobalt chloride, 50mg；Copper sulphate, 10mg；Ferric sulfate, 80mg；Zinc sulfate, 50mg；Magnesium sulfate, 1200mg；Calcium dihydrogen phosphate, 3000mg；Chlorination Sodium, 100mg；Zeolite powder, 5.51g.
The crude protein content that the composite fish bait for red-fin Eastern-Globefish measures is 46%, crude fat content 9%.
Embodiment 4
The present embodiment provides composite fish bait for red-fin Eastern-Globefish composite functional additive, the red fin east of raising described in example Square Puffer compound feed prescription is made of the raw material of following mass fraction: white fish meal, and 46%；Liquid fish, 5%；Brewer's yeast, 3%；Gluten, 2%；Corn protein powder, 1%；Soy protein concentrate, 1%；Wheat flour, 31.68%；Fish oil, 2.7%；Phosphorus Rouge, 1.5%；Calcium dihydrogen phosphate, 1.5%；Vitamin mixtures, 1%；Mineral mixture, 1%；Composite functional additive, 1.62%.
The composite functional additive is by vitamin C, vitamin E, inositol, taurine and Na2SeO3Composition, respectively at Dividing the mass fraction in feed is respectively 0.5%, 0.3%, 0.02%, 0.6% and 0.05%.
Vitamin mixtures (the mgkg-1Feed) it include: thiamine, 25mg；Riboflavin, 45mg；Puridoxine hydrochloride, 20mg；Vitamin B12, 0.1mg；Vitamin K3, 10mg；Inositol, 800mg；Pantothenic acid, 60mg；Niacin, 200mg；Folic acid, 20mg； Biotin, 1.20mg；Vitamin A, 32mg；Vitamin D, 5mg；Vitamin E, 120mg；Wheat-middlings 8.67g.
Mineral element mixture (the mgkg-1Feed) it include: sodium fluoride, 2mg；Potassium iodide, 0.8mg；Cobalt chloride, 50mg；Copper sulphate, 10mg；Ferric sulfate, 80mg；Zinc sulfate, 50mg；Magnesium sulfate, 1200mg；Calcium dihydrogen phosphate, 3000mg；Chlorination Sodium, 100mg；Zeolite powder, 5.51g.
The crude protein content that the composite fish bait for red-fin Eastern-Globefish measures is 46%, crude fat content 9%.
Using 4 groups of mixed feeds of above-described embodiment 3 and embodiment, as a control group with the feed of embodiment 1, selection is initial The Fugu rubripes juvenile fish of weight 150g, it is real in the cultivation that 15 DEG C of water temperature of indoor circulating water cultivation systems carry out 10 weeks by a definite date It tests, every group sets 3 repetitions, after experiment, measures the survival, growth, liver index, internal organ index, activities of antioxidant enzymes of fish Etc. indexs.Experimental result is shown in Table 3, table 4 and table 5.Then, 30 tail Fugu rubripes are taken at random for every group, liver is dissected, according to liver Health status determines whether to suffer from winter syndrome, and specific judgment basis is shown in Fig. 1 (color image in visible reference paper), then The disease incidence that winter syndrome is suffered from Fugu rubripes is counted, the result is shown in tables 6.
The growth result of 3. embodiment 1,3 and 4 of table experiment fish
The body index of 4. embodiment 1,3 and 4 of table experiment fish
Liver body ratio (%) Dirty body ratio (%) Coefficient of condition (g/cm3)
Embodiment 1 8.61±1.54b 13.76±0.46b 3.72±0.10
Embodiment 3 7.51±1.54a 11.45±0.72a 4.00±0.29
Embodiment 4 7.26±1.64a 11.38±0.70a 4.16±0.22
ANOVA
P value 0.006 0.029 0.435
5. embodiment 1,3 and 4 of table tests the hepatic antioxidant activity of fish
The morbidity statistics of winter syndrome occurs for 6. embodiment 1,3 of table and 4 Fugu rubripes
Embodiment 1 Embodiment 3 Embodiment 4 ANOVA
Disease incidence (%) 36.67±3.33c 23.33±1.92b 10.00±1.92a P=0.001
By the result of table 3, table 4 and table 5 it is found that compared to the control group, the Fugu rubripes growth of embodiment 4 is significant to be increased, And embodiment 3 and 4 liver body ratio of embodiment and dirty body are than being substantially less than control group；Compared to the control group, hepatic antioxidant activity SOD, CAT and GSH-Px are significantly increased at 4 groups of embodiment, and only the active of GSH-Px significantly increases embodiment 3.These result tables Bright, the feed of embodiment 3 and embodiment 4 is added under the conditions of reducing fat and albumen by addition composite feeding promoting agent and functionality Add agent, not only promotes Fugu rubripes growth, but also significantly improve liver oxidation resistance.In addition, as shown in Table 6, Composite feeding promoting agent and functional additive are added in feed, winter synthesis occurs for the Fugu rubripes for reducing by 13% and 26% respectively Disease incidence is levied, and complex function additive effect is better than composite feeding promoting agent.Therefore, the present embodiment is developed composite feeding promoting agent and Functional additive has reliable effect when coping with Fugu rubripes winter syndrome.
The pilot plant test of 5 Fugu rubripes winter syndrome functional feed of embodiment is verified
In the cement pit of 15 DEG C of indoor water-flowing cultivating systems, indoor culture environment in analog culture production practices, into The pilot experiment of overwintering cultivation in the behavior phase 10 weeks, with existing Fugu rubripes feed (albumen 48%, fat in the market 11%) and iced trash fish compares, and authentication functionality feed copes with the overwintering effect of Fugu rubripes winter syndrome.
The present embodiment provides the Fugu rubripes overwintering breeding function feed integrated by formula, the red fins of the raising Fugu compound feed prescription is made of the raw material of following mass fraction: white fish meal, and 46%；Liquid fish, 5%；Beer ferment Mother, 3%；Gluten, 2%；Corn protein powder, 1%；Soy protein concentrate, 1%；Wheat flour, 31.18%；Fish oil, 2.7%； Phosphatide, 1.5%；Calcium dihydrogen phosphate, 1.5%；Vitamin mixtures, 1%；Mineral mixture, 1%；Composite feeding promoting agent, 0.5%；Composite functional additive, 1.62%.
The composite feeding promoting agent is alanine, glycine and DMPT, and mass fraction of each ingredient in feed is respectively 0.125%, 0.125%, 0.25%.
The composite functional additive is vitamin C, vitamin E, inositol, taurine and Na2SeO3, each ingredient exists Mass fraction in feed is respectively 0.5%, 0.3%, 0.02%, 0.6% and 0.05%.
Vitamin mixtures (the mgkg-1Feed) it include: thiamine, 25mg；Riboflavin, 45mg；Puridoxine hydrochloride, 20mg；Vitamin B12, 0.1mg；Vitamin K3, 10mg；Inositol, 800mg；Pantothenic acid, 60mg；Niacin, 200mg；Folic acid, 20mg； Biotin, 1.20mg；Vitamin A, 32mg；Vitamin D, 5mg；Vitamin E, 120mg；Wheat-middlings 8.67g.
Mineral element mixture (the mgkg-1Feed) it include: sodium fluoride, 2mg；Potassium iodide, 0.8mg；Cobalt chloride, 50mg；Copper sulphate, 10mg；Ferric sulfate, 80mg；Zinc sulfate, 50mg；Magnesium sulfate, 1200mg；Calcium dihydrogen phosphate, 3000mg；Chlorination Sodium, 100mg；Zeolite powder, 5.51g.
The crude protein content that the composite fish bait for red-fin Eastern-Globefish measures is 46%, crude fat content 9%.
In the present embodiment, the preparation of the mixed feed uses machine identical with commercial feed and manufacturing process, briefly Be described as follows: by all raw material meals it is broken after be sufficiently mixed, after pulverizing, modulation curing, extruding granulation obtains after dry The mixed feed.
The cultivation object of the present embodiment selects the Fugu rubripes juvenile fish of original body mass 130g or so, after experiment, surveys Determine the indexs such as survival, growth, liver index, internal organ index, the activities of antioxidant enzymes of fish.Experimental result is shown in Table 7, table 8 and table 9. Then, 30 tail Fugu rubripes are selected at random for every group, liver is dissected, according to the criterion (colour in visible reference paper of Fig. 1 Picture), the disease incidence of winter syndrome occurs for statistics Fugu rubripes, and the result is shown in tables 10.
7. pilot plant test growth result of table
8. pilot plant test Fugu rubripes body index of table
Liver body ratio (%) Dirty body ratio (%) Coefficient of condition (g/cm3)
Embodiment 5 7.00±2.26a 11.10±0.68a 4.37±0.21
Commercial feed 8.04±2.29ab 12.61±0.79ab 4.26±0.38
Iced trash fish 8.93±2.13b 15.04±0.94b 4.39±0.37
ANOVA
P value 0.008 0.012 0.959
9. pilot plant test hepatic antioxidant activity of table
The morbidity statistics of 10. Fugu rubripes of table generation winter syndrome
Embodiment 5 Commercial feed Iced trash fish ANOVA
Disease incidence (%) 4.44±1.11a 32.22±2.22c 23.33±1.92b P=0.000
Above-mentioned pilot scale the experimental results showed that, the Fugu rubripes growth performance of ingestion function feed (embodiment 5) is significant Higher than commercial feed group, and with iced trash fish group without significant difference.Meanwhile functional feed is reducing Fugu rubripes liver and interior While dirty fat content, liver oxidation resistance is improved.Finally, to reply Fugu rubripes winter syndrome occurs for reflection On, functional feed reduces 28% He of winter syndrome disease incidence of Fugu rubripes compared with commercial feed and iced trash fish respectively 19%, so that the Fugu rubripes survival rate of ingestion function feed be made to significantly improve.
Technical solution of the present invention is not limited to feed base formula in embodiment, and basic components therein are in art technology It is adjusted the ratio of each composition in personnel's common sense, and on the basis of the basic components, is replaced with fish meal or hydrolyzed fish protein powder For Activities of Some Plants albumen, vegetable oil consumption is reduced, keeps protein content 46% on the whole, fat content is grown 9% With the indifference of basal feed, composite feeding promoting agent and composite functional additive of the invention are added on this basis, then it is answered Reduction disease incidence effect of the present invention can be reached to the effect of Fugu rubripes winter syndrome.

Claims (4)

1. a kind of functional feed for coping with Fugu rubripes winter syndrome, it is characterised in that the functional feed is existing red It is dense with fish meal and liquid fish substitution part Gluten, corn protein powder and soybean on the basis of fin dongfang globe fish mixed feed Pix protein powder, reducing the protein content in composite fish bait for red-fin Eastern-Globefish by 2 percentage points is 46%；It is reduced in formula simultaneously Vegetable oil usage amount, reducing the fat content in composite fish bait for red-fin Eastern-Globefish by 2 percentage points is 9%.
2. a kind of functional feed for coping with Fugu rubripes winter syndrome according to claim 1, it is characterised in that institute A kind of composite feeding promoting agent is added in the functional feed stated, the composite feeding promoting agent includes alanine, glycine and thio beet Alkali, the mass ratio of 3 kinds of ingredients are 1:1:2, and mass fraction of the composite feeding promoting agent in functional feed is 0.5%.
3. a kind of functional feed for coping with Fugu rubripes winter syndrome according to claim 1, it is characterised in that institute A kind of composite functional additive is added in the functional feed stated, the composite functional additive includes vitamin C, vitamin E, inositol, taurine and Na2SeO3, the mass ratio of 5 kinds of ingredients is 50:30:2:60:5, and the composite functional additive exists Mass fraction in functional feed is 1.62%.
4. a kind of functional feed for coping with Fugu rubripes winter syndrome according to claim 1, it is characterised in that institute The ingredient and mass fraction for stating functional feed are respectively as follows: white fish meal, and 46%；Liquid fish, 5%；
Brewer's yeast, 3%；Gluten, 2%；Corn protein powder, 1%；Soy protein concentrate, 1%；Wheat flour, 31.18%；Fish Oil, 2.7%；Phosphatide, 1.5%；Calcium dihydrogen phosphate, 1.5%；Vitamin mixtures, 1%；Mineral mixture, 1%；It is compound to lure Food agent, 0.5%；Composite functional additive, 1.62%；
The composite feeding promoting agent is alanine, glycine and sulfobetaine, and the mass ratio of 3 kinds of ingredients is 1:1:2；
The composite functional additive is vitamin C, vitamin E, inositol, taurine and Na2SeO3, the quality of 5 kinds of ingredients Ratio is 50:30:2:60:5；
The vitamin mixtures are the ingredient contained in per kilogram functional feed and quality includes: thiamine, 25mg；Core yellow Element, 45mg；Puridoxine hydrochloride, 20mg；Vitamin B12, 0.1mg；Vitamin K3, 10mg；Inositol, 800mg；Pantothenic acid, 60mg；Cigarette Acid, 200mg；Folic acid, 20mg；Biotin, 1.20mg；Vitamin A, 32mg；Vitamin D, 5mg；Vitamin E, 120mg；Wheat-middlings 8.67g；
The mineral element mixture is the ingredient contained in per kilogram functional feed and quality includes: sodium fluoride, 2mg；Iodate Potassium, 0.8mg；Cobalt chloride, 50mg；Copper sulphate, 10mg；Ferric sulfate, 80mg；Zinc sulfate, 50mg；Magnesium sulfate, 1200mg；Di(2-ethylhexyl)phosphate Hydrogen calcium, 3000mg；Sodium chloride, 100mg；Zeolite powder, 5.51g.
CN201910724740.2A 2019-08-07 2019-08-07 A kind of functional feed for coping with Fugu rubripes winter syndrome Pending CN110313561A (en)

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Application publication date: 20191011