CN102841007A - Semen diluent convenient to count duck sperms and application method thereof - Google Patents
Semen diluent convenient to count duck sperms and application method thereof Download PDFInfo
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- CN102841007A CN102841007A CN2012103250724A CN201210325072A CN102841007A CN 102841007 A CN102841007 A CN 102841007A CN 2012103250724 A CN2012103250724 A CN 2012103250724A CN 201210325072 A CN201210325072 A CN 201210325072A CN 102841007 A CN102841007 A CN 102841007A
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Abstract
The invention discloses semen diluent convenient to count duck sperms and an application method of the semen diluent. The semen diluent is composed of the following components: 40 to 60g of NaHCO3 (sodium bicarbonate), 5 to 15mL of 35% of formaldehyde, 8 to 12g of gentian violet, 5 to 15mL of 96% of alcohol, and normal saline added to 1000mL. The semen after being diluted by the diluent is capable of not only quickly killing the sperms, but also improving definition when a microscopic examination is carried out, so that quantity of the duck sperms is accurately counted, and density of the drake sperms is detected, thereby providing an important reference on use of the drakes, dilution, storage and transportation of semen.
Description
Technical field
The invention belongs to duck technology of artificial insemination field, be specifically related to a kind of semen diluent and method of application thereof of being convenient to the duck sperm count.
Background technology
The evaluation of duck semen quality can be identified the quality of semen quality, confirms its value; It is the only resource of check semen dilution, storage and transport effect; Be requisite important step in the duck artificial insemination process, and the density of duck sperm is to weigh an important indicator of semen quality quality.The sperm count method is the important method that sperm concentration detects, and in order accurately to add up sperm quantity, (hemacytometry) should be pressed seminal fluid the multiple dilution of 1:200 earlier when the number sperm count.Only adopt physiological saline that seminal fluid is diluted generally speaking, can not kill sperm like this, most of sperms also are in the state of motion at 400 times microscopically, are unfavorable for the counting of sperm, and then can't measure the density of duck sperm accurately.
Summary of the invention
In order to solve the above-mentioned defective of prior art, the object of the present invention is to provide a kind of semen diluent and method of application thereof of being convenient to the duck sperm count.Seminal fluid after this diluted; Not only can kill sperm rapidly, the sharpness in the time of also can improving microscopy, and then count the quantity of duck sperm accurately; Measure kind of the density of drake sperm, for utilization, the dilution of seminal fluid, the storage and transport of kind of drake provide important reference.
For realizing above-mentioned purpose, the present invention adopts following technical scheme:
A kind of semen diluent of being convenient to the duck sperm count is made up of the component of following content: NaHCO
340-60g, 35% formaldehyde 5-15mL, gentian violet 8-12g, 96% alcohol 5-15mL adds physiological saline to 1000mL.NaHCO in this dilution
3Mucus in the solubilized seminal fluid, 35% formaldehyde and 96% alcohol can play the effect of killing rapidly and suppressing sperm motility, the sharpness when gentian violet can be improved microscopy.
A kind of aforesaid method of application of being convenient to the semen diluent of duck sperm count; Draw 10 μ L seminal fluid in glass cylinder with the liquid-transfering gun of range 10 μ L; Use range to draw dilution that 2mL configures in glass cylinder again, and come resorption to beat under going up it is mixed as the disposable syringe of 2mL; The seminal fluid of drawing after 20 μ L dilute with liquid-transfering gun drops in the edge between tally and cover glass, and seminal fluid is infiltrated in the counting chamber automatically, and seminal fluid is added on the hemacytometer will place 1min at least, makes the sperm deposition, so that counting.
Beneficial effect of the present invention: the seminal fluid after this diluted; Not only can kill sperm rapidly; Sharpness in the time of also can improving microscopy; And then count the quantity of duck sperm accurately, measure kind of the density of drake sperm, for utilization, the dilution of seminal fluid, the storage and transport of kind of drake provide important reference.
Description of drawings
Fig. 1 is the state of sperm under 400 power microscopes after the diluted.
Embodiment
Embodiment 1
The configuration of semen diluent: take by weighing NaHCO respectively
340g, gentian violet 8g add the formaldehyde of 5ml35% and 96% alcohol in the 250ml beaker, stir with glass bar, and the dissolving back moves in the volumetric flask of 1000ml fully, adds physiological saline to scale and shakes up.
Draw 10 μ l seminal fluid in glass cylinder with the liquid-transfering gun of range 10 μ l, use range to draw dilution that 2ml configures in glass cylinder again, and come resorption to beat under going up it is mixed as the disposable syringe of 2ml.The seminal fluid of drawing after 20 μ l dilute with liquid-transfering gun drops in the edge between tally and cover glass, and seminal fluid is infiltrated in the counting chamber automatically, and seminal fluid is added on the hemacytometer will place 1min at least, makes the sperm deposition, so that counting.
Embodiment 2
The configuration of semen diluent: take by weighing NaHCO respectively
360g, gentian violet 12g add the formaldehyde of 15ml35% and 96% alcohol in the 250ml beaker, stir with glass bar, and the dissolving back moves in the volumetric flask of 1000ml fully, adds physiological saline to scale and shakes up.
Draw 10 μ l seminal fluid in glass cylinder with the liquid-transfering gun of range 10 μ l, use range to draw dilution that 2ml configures in glass cylinder again, and come resorption to beat under going up it is mixed as the disposable syringe of 2ml.The seminal fluid of drawing after 20 μ l dilute with liquid-transfering gun drops in the edge between tally and cover glass, and seminal fluid is infiltrated in the counting chamber automatically, and seminal fluid is added on the hemacytometer will place 1min at least, makes the sperm deposition, so that counting.
Embodiment 3
The configuration of semen diluent: take by weighing NaHCO respectively
350g, gentian violet 10g add the formaldehyde of 10ml35% and 96% alcohol in the 250ml beaker, stir with glass bar, and the dissolving back moves in the volumetric flask of 1000ml fully, adds physiological saline to scale and shakes up.
Draw 10 μ l seminal fluid in glass cylinder with the liquid-transfering gun of range 10 μ l, use range to draw dilution that 2ml configures in glass cylinder again, and come resorption to beat under going up it is mixed as the disposable syringe of 2ml.The seminal fluid of drawing after 20 μ l dilute with liquid-transfering gun drops in the edge between tally and cover glass, and seminal fluid is infiltrated in the counting chamber automatically, and seminal fluid is added on the hemacytometer will place 1min at least, makes the sperm deposition, so that counting.
Fig. 1 is the state of sperm under 400 power microscopes after the diluted.Annotate: the formula that calculates sperm count is: the sperm sum of 1ml=(5 sperm sum/80 lattices that big grid is interior) * 400 * 10 * 1000 * extension rate
The above is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. semen diluent of being convenient to the duck sperm count, it is characterized in that: described semen diluent is made up of the component of following content: NaHCO
340-60g, 35% formaldehyde 5-15mL, gentian violet 8-12g, 96% alcohol 5-15mL adds physiological saline to 1000mL.
2. method of application of being convenient to the semen diluent of duck sperm count as claimed in claim 1; It is characterized in that: draw 10 μ L seminal fluid in glass cylinder with the liquid-transfering gun of range 10 μ L; Use range to draw dilution that 2mL configures in glass cylinder again, and come resorption to beat under going up it is mixed as the disposable syringe of 2mL; The seminal fluid of drawing after 20 μ L dilute with liquid-transfering gun drops in the edge between tally and cover glass, and seminal fluid is infiltrated in the counting chamber automatically, and seminal fluid is added on the hemacytometer will place 1min at least, makes the sperm deposition, so that counting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103250724A CN102841007A (en) | 2012-09-05 | 2012-09-05 | Semen diluent convenient to count duck sperms and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103250724A CN102841007A (en) | 2012-09-05 | 2012-09-05 | Semen diluent convenient to count duck sperms and application method thereof |
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| CN102841007A true CN102841007A (en) | 2012-12-26 |
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| CN2012103250724A Pending CN102841007A (en) | 2012-09-05 | 2012-09-05 | Semen diluent convenient to count duck sperms and application method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070162A (en) * | 2013-01-30 | 2013-05-01 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
Citations (5)
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| CN1145638A (en) * | 1994-01-05 | 1997-03-19 | 基因切变有限公司 | Ribozymes targeting retroviral packaging sequence expression constructs and recombinant retroviruses containing such constructs |
| CN1631124A (en) * | 2003-12-24 | 2005-06-29 | 甘孜州家畜改良站 | Method for making frozen spermatozoon of yak |
| WO2010069335A2 (en) * | 2008-12-19 | 2010-06-24 | Københavns Universitet | Diagnosis of endometritis |
| CN101948815A (en) * | 2010-07-12 | 2011-01-19 | 中国人民解放军军事医学科学院微生物流行病研究所 | EV-71(Enterovirus 71) type viral plaque purification method |
| US7958848B2 (en) * | 2009-05-30 | 2011-06-14 | Mahmoud Bahmani | Method for artificial breeding of farmed sturgeon |
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2012
- 2012-09-05 CN CN2012103250724A patent/CN102841007A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1145638A (en) * | 1994-01-05 | 1997-03-19 | 基因切变有限公司 | Ribozymes targeting retroviral packaging sequence expression constructs and recombinant retroviruses containing such constructs |
| CN1631124A (en) * | 2003-12-24 | 2005-06-29 | 甘孜州家畜改良站 | Method for making frozen spermatozoon of yak |
| WO2010069335A2 (en) * | 2008-12-19 | 2010-06-24 | Københavns Universitet | Diagnosis of endometritis |
| WO2010069335A3 (en) * | 2008-12-19 | 2010-08-19 | Københavns Universitet | Diagnosis of endometritis |
| US7958848B2 (en) * | 2009-05-30 | 2011-06-14 | Mahmoud Bahmani | Method for artificial breeding of farmed sturgeon |
| CN101948815A (en) * | 2010-07-12 | 2011-01-19 | 中国人民解放军军事医学科学院微生物流行病研究所 | EV-71(Enterovirus 71) type viral plaque purification method |
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| 辛清武: "优质中型肉用鸭的精液特性及人工授精方法研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070162A (en) * | 2013-01-30 | 2013-05-01 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
| CN103070162B (en) * | 2013-01-30 | 2014-10-08 | 福建省农业科学院畜牧兽医研究所 | Muscovy duck semen diluent |
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Inventor after: Zheng Nenzhu Inventor after: Xin Qingwu Inventor after: Zhu Zhiming Inventor after: Jiu Zhongwei Inventor before: Xin Qingwu Inventor before: Zheng Nenzhu Inventor before: Zhu Zhiming Inventor before: Jiu Zhongwei |
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Free format text: CORRECT: INVENTOR; FROM: XIN QINGWU ZHENG NENZHU ZHU ZHIMING MIAO ZHONGWEI TO: ZHENG NENZHU XIN QINGWU ZHU ZHIMING MIAO ZHONGWEI |
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Application publication date: 20121226 |