CN105994249A - Preservation method for benthonic animal sample - Google Patents
Preservation method for benthonic animal sample Download PDFInfo
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- CN105994249A CN105994249A CN201610410150.9A CN201610410150A CN105994249A CN 105994249 A CN105994249 A CN 105994249A CN 201610410150 A CN201610410150 A CN 201610410150A CN 105994249 A CN105994249 A CN 105994249A
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- CN
- China
- Prior art keywords
- sample
- benthonic animal
- zoobenthos
- specimen
- animal sample
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a preservation method for a benthonic animal sample. The preservation method includes the steps that the benthonic animal sample is soaked in a formaldehyde solution and then in double-distilled water, then washed with an alcoholic solution, dried and placed in glassware, neutral gum is added, and the sample is dried again. First, the benthonic animal sample is soaked in the formaldehyde solution for sterilization, and the benthonic animal is made to die fast and kept in a stiff state to facilitate follow-up observation; then the benthonic animal sample is soaked in the double-distilled water and washed with ethyl alcohol, finally the neutral gum is added into the glassware, and the benthonic animal sample is dried again and manufactured into a specimen. The specimen can be preserved for a long time, and the specimen with the neutral gun removed by adding xylene can be used for molecular DNA analytical research, comparative research of benthonic animal classification and identification and the like.
Description
Technical field
The invention belongs to Sample preservation technical field, particularly relate to the store method of a kind of zoobenthos sample.
Background technology
Zoobenthos refers to live in the invertebrates of water bottom, including in water body, deposit and attached
The all animals in deposit surface, of a great variety, more than 1,000,000 kinds.Zoobenthos is because having quantity
Many, widely distributed, to the feature such as environmental change sensitivity, biocycle length, ability of migrating be more weak frequently as
Environmental monitoring index.Zoobenthos is in the 3rd trophic level, for the circulation of ecosystem material and energy stream
Dynamic have important effect.Along with the research in terms of zoobenthos increases day by day and gos deep into, zoobenthos mark
This collection and preservation seem significant for research in this respect.
The most conventional method is the most single, typically in the formalin (i.e. formalin) of 5wt%～10wt%
Middle preservation.But, in formalin after soak time length, zoobenthos is oxidized easily, and causes
It decomposes or corrupt, loses preservation meaning.And, formaldehyde toxicity is big, and volatility is relatively strong, and teratogenesis causes
Cancer effect is obvious, and in air, concentration reaches 30mg/m3Time, meeting causing death immediately, Long contact time is right
Health effect is bigger.
Summary of the invention
In view of this, it is an object of the invention to provide the store method of a kind of zoobenthos sample, this
The method of bright offer can preserve zoobenthos sample for a long time, and will not impact human health.
The invention provides the store method of a kind of zoobenthos sample, including:
Soak in distilled water after zoobenthos sample is soaked in formalin, then use alcoholic solution
Washing, dries and is placed in glass drying oven, adds neutral gum, again dries.
The present invention, first by zoobenthos sample soaking sterilization in formalin, makes zoobenthos fast simultaneously
Speed is dead and keeps spasticity, it is simple to later observation；Then soak in distilled water, then wash with ethanol
Wash, in glass drying oven, finally add neutral gum or transparent nail polish, after again drying, make specimen,
Can not only preserve for a long time, and add the sample after dimethylbenzene removes neutral gum and can be used for molecular dna
Analysis and research, for the comparative study etc. of zoobenthos taxonomic identification.
After the present invention gathers acquisition zoobenthos sample, it is placed in formalin immersion.In the present invention
In, the concentration of described formalin is preferably 35wt%～45wt%, more preferably 40wt%.In formalin
The time of middle immersion is preferably 50s～70s, more preferably 60s.The formaldehyde of high concentration makes zoobenthos rapid
Death, the most stiff property is dead, it is simple to later observation, also has been able to the sterilization operation to specimen simultaneously.
Being soaked in distilled water by zoobenthos sample after soaking in formalin, soak time is preferably
3min～7min, more preferably 5min.
After distilled water soaks, by zoobenthos sample ethanol wash, the volumetric concentration of described ethanol
It is preferably 50%～70%, more preferably 60%.
Zoobenthos sample is dried after washing by ethanol, removes moisture removal and ethanol.The present invention is excellent
Choosing is dried in an oven, and the temperature of described drying is preferably 25 DEG C～35 DEG C, more preferably 30 DEG C.
After drying, zoobenthos sample is placed in transparent glass drying oven, after flattening Each part, slow
Slowly add neutral gum, can preserve for a long time after again drying.In the present invention, described again dry excellent
Choosing is the most slowly dried, and the described temperature again dried is preferably 15 DEG C～25 DEG C, more preferably 20 DEG C.
The zoobenthos sample obtained through above-mentioned process is placed in being dried shady and cool indoor long-term observation, also
Dimethylbenzene removing neutral gum can be added be sampled or sample dissection.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that institute
The embodiment described is only a part of embodiment of the present invention rather than whole embodiments.Based on this
Embodiment in bright, those of ordinary skill in the art are obtained under not making creative work premise
Every other embodiment, broadly falls into the scope of protection of the invention.
Comparative example 1
Gather zoobenthos apparent movement Fu (one of Ephemerida, belong to zoobenthos), put into 5wt%～10wt%
Formalin in soak, labelled, the indoor preservation that is dried in cool place.
After preserving half a year, the fresh and alive sample before relatively preserving has occurred and that significant change, specimen have been decomposed,
Structure seems smudgy change.
Testing through DNA molecular, result shows, it can not be normally used for extracting DNA.
Embodiment 2
Gather zoobenthos Dulicidae adult (belonging to zoobenthos), put in the formalin of 40wt% and soak
1 minute, then take out zoobenthos sample, soak 5 minutes in distilled water, take out sample at 60wt%
Alcoholic solution takes out rapidly after washing, after 30 DEG C of baking ovens remove moisture and the ethanol on surface, is placed in
In transparent glass drying oven, after flattening Each part, it is slowly added neutral gum.By facing of making
Time specimen be placed in 20 DEG C of baking ovens and slowly dry, labelled, the indoor preservation that is dried in cool place.
After preserving 2 years, specimen does not changes substantially.
In the specimen obtained, add dimethylbenzene, remove collecting sample after neutral gum, and for DNA molecular
Experiment.Result shows, it can be used for extracting DNA.
The above is only the preferred embodiment of the present invention, it is noted that for the common skill of the art
For art personnel, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications,
These improvements and modifications also should be regarded as protection scope of the present invention.
Claims (7)
1. a store method for zoobenthos sample, including:
Soak in distilled water after zoobenthos sample is soaked in formalin, then use alcoholic solution
Washing, dries and is placed in glass drying oven, adds neutral gum, again dries.
Store method the most according to claim 1, it is characterised in that the concentration of described formalin
For 35wt%～45wt%.
Store method the most according to claim 2, it is characterised in that soak in formalin
Time is 50s～70s.
Method the most according to claim 3, it is characterised in that soak 3min～7min in distilled water.
Method the most according to claim 4, it is characterised in that the volumetric concentration of described ethanol is
50%～70%.
Method the most according to claim 5, it is characterised in that the temperature of described drying is 25 DEG C
～35 DEG C.
Method the most according to claim 6, it is characterised in that the described temperature again dried is 15 DEG C
～25 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610410150.9A CN105994249A (en) | 2016-06-12 | 2016-06-12 | Preservation method for benthonic animal sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610410150.9A CN105994249A (en) | 2016-06-12 | 2016-06-12 | Preservation method for benthonic animal sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105994249A true CN105994249A (en) | 2016-10-12 |
Family
ID=57090238
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610410150.9A Pending CN105994249A (en) | 2016-06-12 | 2016-06-12 | Preservation method for benthonic animal sample |
Country Status (1)
| Country | Link |
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| CN (1) | CN105994249A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108207929A (en) * | 2018-02-06 | 2018-06-29 | 国家海洋局北海环境监测中心 | A kind of store method of zoobenthos sample |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810160A (en) * | 2010-04-13 | 2010-08-25 | 南京医科大学 | Soaking preservation solution for removing formaldehyde from human anatomic specimen and preparation method thereof |
| CN105248410A (en) * | 2015-11-17 | 2016-01-20 | 广东海洋大学 | Preservation method for biological specimen |
-
2016
- 2016-06-12 CN CN201610410150.9A patent/CN105994249A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810160A (en) * | 2010-04-13 | 2010-08-25 | 南京医科大学 | Soaking preservation solution for removing formaldehyde from human anatomic specimen and preparation method thereof |
| CN105248410A (en) * | 2015-11-17 | 2016-01-20 | 广东海洋大学 | Preservation method for biological specimen |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108207929A (en) * | 2018-02-06 | 2018-06-29 | 国家海洋局北海环境监测中心 | A kind of store method of zoobenthos sample |
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Application publication date: 20161012 |