CN105850897B - A kind of artificial fecundation method of Tetrastichus septentrionalis - Google Patents
A kind of artificial fecundation method of Tetrastichus septentrionalis Download PDFInfo
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- CN105850897B CN105850897B CN201610332714.1A CN201610332714A CN105850897B CN 105850897 B CN105850897 B CN 105850897B CN 201610332714 A CN201610332714 A CN 201610332714A CN 105850897 B CN105850897 B CN 105850897B
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- 238000000034 method Methods 0.000 title claims abstract description 15
- 241001546457 Tetrastichus Species 0.000 title claims description 40
- 241000382353 Pupa Species 0.000 claims abstract description 93
- 241000525857 Micromelalopha Species 0.000 claims abstract description 44
- 241001504501 Troglodytes Species 0.000 claims abstract description 44
- 230000003071 parasitic effect Effects 0.000 claims abstract description 34
- 239000000284 extract Substances 0.000 claims abstract description 19
- 230000024241 parasitism Effects 0.000 claims abstract description 17
- 239000000126 substance Substances 0.000 claims abstract description 16
- 238000012216 screening Methods 0.000 claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000004033 plastic Substances 0.000 claims description 12
- 229920003023 plastic Polymers 0.000 claims description 12
- 238000009423 ventilation Methods 0.000 claims description 7
- 238000010792 warming Methods 0.000 claims description 7
- 239000004677 Nylon Substances 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- 241000317929 Chalcididae Species 0.000 abstract description 13
- 241001531327 Hyphantria cunea Species 0.000 abstract description 6
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 241000607479 Yersinia pestis Species 0.000 abstract description 5
- 241000255777 Lepidoptera Species 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 2
- 241000256844 Apis mellifera Species 0.000 abstract 2
- 241000219000 Populus Species 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- BQPPJGMMIYJVBR-UHFFFAOYSA-N (10S)-3c-Acetoxy-4.4.10r.13c.14t-pentamethyl-17c-((R)-1.5-dimethyl-hexen-(4)-yl)-(5tH)-Delta8-tetradecahydro-1H-cyclopenta[a]phenanthren Natural products CC12CCC(OC(C)=O)C(C)(C)C1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21C BQPPJGMMIYJVBR-UHFFFAOYSA-N 0.000 description 1
- CHGIKSSZNBCNDW-UHFFFAOYSA-N (3beta,5alpha)-4,4-Dimethylcholesta-8,24-dien-3-ol Natural products CC12CCC(O)C(C)(C)C1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21 CHGIKSSZNBCNDW-UHFFFAOYSA-N 0.000 description 1
- XYTLYKGXLMKYMV-UHFFFAOYSA-N 14alpha-methylzymosterol Natural products CC12CCC(O)CC1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21C XYTLYKGXLMKYMV-UHFFFAOYSA-N 0.000 description 1
- FPTJELQXIUUCEY-UHFFFAOYSA-N 3beta-Hydroxy-lanostan Natural products C1CC2C(C)(C)C(O)CCC2(C)C2C1C1(C)CCC(C(C)CCCC(C)C)C1(C)CC2 FPTJELQXIUUCEY-UHFFFAOYSA-N 0.000 description 1
- 241000255978 Antheraea pernyi Species 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- BKLIAINBCQPSOV-UHFFFAOYSA-N Gluanol Natural products CC(C)CC=CC(C)C1CCC2(C)C3=C(CCC12C)C4(C)CCC(O)C(C)(C)C4CC3 BKLIAINBCQPSOV-UHFFFAOYSA-N 0.000 description 1
- LOPKHWOTGJIQLC-UHFFFAOYSA-N Lanosterol Natural products CC(CCC=C(C)C)C1CCC2(C)C3=C(CCC12C)C4(C)CCC(C)(O)C(C)(C)C4CC3 LOPKHWOTGJIQLC-UHFFFAOYSA-N 0.000 description 1
- CAHGCLMLTWQZNJ-UHFFFAOYSA-N Nerifoliol Natural products CC12CCC(O)C(C)(C)C1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21C CAHGCLMLTWQZNJ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- QBSJHOGDIUQWTH-UHFFFAOYSA-N dihydrolanosterol Natural products CC(C)CCCC(C)C1CCC2(C)C3=C(CCC12C)C4(C)CCC(C)(O)C(C)(C)C4CC3 QBSJHOGDIUQWTH-UHFFFAOYSA-N 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- CAHGCLMLTWQZNJ-RGEKOYMOSA-N lanosterol Chemical compound C([C@]12C)C[C@@H](O)C(C)(C)[C@H]1CCC1=C2CC[C@]2(C)[C@H]([C@H](CCC=C(C)C)C)CC[C@@]21C CAHGCLMLTWQZNJ-RGEKOYMOSA-N 0.000 description 1
- 229940058690 lanosterol Drugs 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses the artificial fecundation methods that a kind of black stick nibbles chalcid fly, include the following steps：(a) Hosts, the field acquisition of (b) micromelalopha troglodyte pupa and screening, the culture of (c) honeybee kind, the extraction of (d) information substance, the processing of (e) tussah chrysalis, (f) is selected to connect bee, (g) culture development.The present invention impregnates not parasitic pupa by alcohol and obtains pupa table extract, is then sprayed onto on tussah chrysalis by the method manually sprayed, induces black stick to nibble chalcid fly parasitism, improve parasitic rate；Chalcid fly is nibbled using the black stick of indoor large-scale breeding, practical technological means is provided for lepidoptera pests such as woodland biological control fall webworms, micromelalopha troglodytes.
Description
Technical field
The present invention relates to the artificial fecundation methods that chalcid fly is nibbled in insect reproduction field more particularly to a kind of black stick.
Background technology
Tetrastichus septentrionalis Tetrastichus septentrionalis Yang are fall webworms, micromelalopha troglodyte, poplar
The important parasite in a variety of Lepidoptera leafeating insect pupa time such as boat moth is fanned, it is more universal in the distribution of northern China, it is squama
A kind of relatively common natural enemy insect in wing mesh pest pupa time, general every piece of fall webworms pupa, which can sprout wings, bee 86-349 heads, puts down
Equal 160.The small bee imago individual is larger, and body is strong, and ability of circling in the air is strong, and vitality is strong, can survive at ambient temperature 15d with
On, natural parasitic rate is sometime up to 12%~24% in fall webworms pupal cell, has in terms of biological control and utilizes well
Foreground.
Tussah (Antheraea pernyi Guerin-Meneville), which can be used for breeding Zhou Shi, nibbles a variety of pupas such as chalcid fly
Parasitic wasp.Tussah chrysalis may have stronger parasitizing ability as Hosts to parasitic wasp, and in terms of preserving with rejuvenation
Advantage.And the relevant technologies also comparative maturity such as it obtains, preserves, inoculation.Up to the present, also and have no favorably with tussah chrysalis and
Other describe the document report of biological control Tetrastichus septentrionalis.
Invention content
It is woodland biological control fall webworms, poplar the purpose of the present invention is nibbling chalcid fly using the indoor black stick of large-scale breeding
The lepidoptera pests such as bateau moth provide practical technological means, provide the artificial fecundation method that a kind of black stick nibbles chalcid fly.
The technical scheme is that realize in the following manner：
A kind of artificial fecundation method of Tetrastichus septentrionalis, includes the following steps：
(a) Hosts are selected：It using northeast tussah autumn pupa as Hosts, is placed in freezer and stores, temperature control exists
4-6 DEG C, relative humidity is maintained at 70%-80%；It screens of fine quality, fresh, individual big northeast tussah autumn pupa and carries out warming cocoons, pass through
It is spare that 2d is gradually warming up to environment temperature；
(b) field acquisition and screening of micromelalopha troglodyte pupa：Micromelalopha troglodyte pupa is acquired in nature, and to micromelalopha troglodyte parasitism
Pupa with the not parasitic pupa of micromelalopha troglodyte screen spare；Micromelalopha troglodyte parasitism pupal cell table colour-darkening, pupal cell internode elongate, and poplar is small
The not parasitic pupal cell table of boat moth is vivid；
(c) kind bee culture：Micromelalopha troglodyte parasitism pupa is put into Tube propagation, Tetrastichus septentrionalis kind bee is used as after emergence
It is spare；
(d) information substance extracts：Micromelalopha troglodyte is not posted with the concentration of 20-40 heads/100ml with the alcohol of 40%-50%
Raw pupa impregnates 10-15h, forms soak；
(e) northeast tussah autumn pupa is handled：By the soak sprayer containing information substance extract of step (d) to step
Suddenly the northeast tussah autumn pupa of (a) screening is uniformly sprayed, and 3-4h is placed after sprinkling, is dried spare；
(f) bee is connect：The northeast tussah autumn pupa of step (e) is put into plastic culture box, under dark condition, uses small brushes
Gently the Tetrastichus septentrionalis kind bee of step (c) is uniformly swept into box, Tetrastichus septentrionalis kind bee and northeast tussah autumn
The ratio of pupa is 30-50：1, temperature control is at 26~28 DEG C, relative humidity 70~80%, connect bee for 24 hours~48h completes；
(g) culture development：Temperature is controlled at 23~28 DEG C, relative humidity 70%-80% and in the environment of keeping ventilation
Culture development；It is Tetrastichus septentrionalis after emergence.
Above-mentioned technical proposal can do following optimization：
(a) Hosts are selected：It using northeast tussah autumn pupa as Hosts, is placed in freezer and stores, temperature is controlled 5
DEG C, relative humidity is maintained at 75%；
(d) information substance extracts：The not parasitic pupa of micromelalopha troglodyte is impregnated with the concentration of 30/100ml with 45% alcohol
13h forms soak；
(e) northeast tussah autumn pupa is handled：By the soak sprayer containing information substance extract of step (d) to step
Suddenly the northeast tussah autumn pupa of (a) screening is uniformly sprayed, and 3.5h is placed after sprinkling, is dried spare；
(f) bee is connect：The northeast tussah autumn pupa of step (e) is put into plastic culture box, under dark condition, uses small brushes
Gently the Tetrastichus septentrionalis kind bee of step (c) is uniformly swept into box, Tetrastichus septentrionalis kind bee and northeast tussah autumn
The ratio of pupa is 40：1, temperature control meets bee 26h and completes in 27 DEG C, relative humidity 75%；
(g) culture development：Temperature is controlled at 26 DEG C, relative humidity 75% and keeps cultivating development in the environment of ventilation；
It is Tetrastichus septentrionalis after emergence.
Above-mentioned technical proposal can also do following optimization：
The length, width and height that step (f) connects the plastic culture box of bee are respectively 15*10*7cm, and 4 radius 1cm are uniformly opened on upper lid
Round air hole, with 300 mesh nylon wire seals on air hole；The northeast tussah autumn pupa 20 of step (e) is placed per box；It spreads as possible
It is one layer, is no more than two layers.
Above-mentioned technical proposal can also do following optimization：
The plastic culture box is put into wooden numerous beehive, 12 boxes is put into per case, places the northeast toothed oak of step (e) altogether
Silkworm autumn pupa 240.
Beneficial effects of the present invention：
1, chalcid fly is nibbled using the black stick of indoor large-scale breeding, is the squamas wings such as woodland biological control fall webworms, micromelalopha troglodyte
Mesh pest provides practical technological means.A large amount of lanosterol are not contained in parasitic micromelalopha troglodyte pupa table extract
(62.411%), the substance is not present in the parasitic micromelalopha troglodyte pupa table extract crossed.The not parasitic pupa table extraction of high concentration
Object can lure Tetrastichus septentrionalis, and parasitic pupa table extract to chalcid fly without attracting action.The present invention is soaked by alcohol
The not parasitic pupa of bubble obtains pupa table extract, is then sprayed onto on tussah chrysalis by the method manually sprayed, black stick is induced to nibble chalcid fly
Parasitism improves parasitic rate.
2, using the numerous bee box of small volume, numerous beehive space is artificially isolated, bee pupa in unit space is strengthened and contacts, enhancing
Parasitic is mandatory, improves the parasitic rate that black stick nibbles chalcid fly.The opposite parasitic utilization rate improved again to space of single tube, carries
The high bee yield of unit space, reduces cost.The mass artificial propagation of chalcid fly is nibbled suitable for black stick.
3, by controlling temperature and humidity, illumination, good artificial breeding environment has been built, ensure that higher parasitic rate
And bee yield, propagation in scale parasitic rate is up to 80% or more, per pupa bee yield at 1600 or more.
4, it is distributed more widely to nibble chalcid fly for black stick, can native country acquisition, realize the breeding of localization natural enemy, and can parasitic rate it is white in the U.S.
A variety of important lepidoptera pests such as moth, defoliator in Populur, and parasitic rate is higher, Utilization prospects are wide.
Specific implementation mode
Explanation is further explained to the present invention with reference to specific embodiment.
Embodiment 1：A kind of artificial fecundation method of Tetrastichus septentrionalis, includes the following steps：
(a) Hosts are selected：It using northeast tussah autumn pupa as Hosts, is placed in freezer and stores, temperature is controlled 4
DEG C, relative humidity is maintained at 70%；It screens of fine quality, fresh, individual big northeast tussah autumn pupa and carries out warming cocoons, gradually risen by 2d
Temperature is spare to environment temperature；
(b) field acquisition and screening of micromelalopha troglodyte pupa：Micromelalopha troglodyte pupa is acquired in nature, and to micromelalopha troglodyte parasitism
Pupa with the not parasitic pupa of micromelalopha troglodyte screen spare；Micromelalopha troglodyte parasitism pupal cell table colour-darkening, pupal cell internode elongate, and poplar is small
The not parasitic pupal cell table of boat moth is vivid；
(c) kind bee culture：Micromelalopha troglodyte parasitism pupa is put into Tube propagation, Tetrastichus septentrionalis kind bee is used as after emergence
It is spare；
(d) information substance extracts：The not parasitic pupa of micromelalopha troglodyte is impregnated with the concentration of 20/100ml with 40% alcohol
10h forms soak；
(e) northeast tussah autumn pupa is handled：By the soak sprayer containing information substance extract of step (d) to step
Suddenly the northeast tussah autumn pupa of (a) screening is uniformly sprayed, and 3h is placed after sprinkling, is dried spare；
(f) bee is connect：The northeast tussah autumn pupa of step (e) is put into plastic culture box, under dark condition, uses small brushes
Gently the Tetrastichus septentrionalis kind bee of step (c) is uniformly swept into box, Tetrastichus septentrionalis kind bee and northeast tussah autumn
Pupa ratio is 30: 1, and temperature is controlled at 26 DEG C, and relative humidity 70% connects bee and completes for 24 hours；
(g) culture development：Temperature is controlled at 23 DEG C, relative humidity 70% and keeps cultivating development in the environment of ventilation；
It is Tetrastichus septentrionalis after emergence.
Embodiment 2：A kind of artificial fecundation method of Tetrastichus septentrionalis, includes the following steps：
(a) Hosts are selected：It using northeast tussah autumn pupa as Hosts, is placed in freezer and stores, temperature is controlled 6
DEG C, relative humidity is maintained at 80%；It screens of fine quality, fresh, individual big northeast tussah autumn pupa and carries out warming cocoons, gradually risen by 2d
Temperature is spare to environment temperature；
(b) field acquisition and screening of micromelalopha troglodyte pupa：Micromelalopha troglodyte pupa is acquired in nature, and to micromelalopha troglodyte parasitism
Pupa with the not parasitic pupa of micromelalopha troglodyte screen spare；Micromelalopha troglodyte parasitism pupal cell table colour-darkening, pupal cell internode elongate, and poplar is small
The not parasitic pupal cell table of boat moth is vivid；
(c) kind bee culture：Micromelalopha troglodyte parasitism pupa is put into Tube propagation, Tetrastichus septentrionalis kind bee is used as after emergence
It is spare；
(d) information substance extracts：The not parasitic pupa of micromelalopha troglodyte is impregnated with the concentration of 40/100ml with 50% alcohol
15h forms soak；
(e) northeast tussah autumn pupa is handled：By the soak sprayer containing information substance extract of step (d) to step
Suddenly the northeast tussah autumn pupa of (a) screening is uniformly sprayed, and 4h is placed after sprinkling, is dried spare；
(f) bee is connect：The northeast tussah autumn pupa of step (e) is put into plastic culture box, under dark condition, uses small brushes
Gently the Tetrastichus septentrionalis kind bee of step (c) is uniformly swept into box, Tetrastichus septentrionalis kind bee and northeast tussah autumn
The ratio of pupa is 50: 1, and temperature control meets bee 48h and complete in 28 DEG C, relative humidity 80%；
(g) culture development：Temperature is controlled at 28 DEG C, relative humidity 80% and keeps cultivating development in the environment of ventilation；
It is Tetrastichus septentrionalis after emergence.
Embodiment 3：A kind of artificial fecundation method of Tetrastichus septentrionalis, includes the following steps：
(a) Hosts are selected：It using northeast tussah autumn pupa as Hosts, is placed in freezer and stores, temperature is controlled 5
DEG C, relative humidity is maintained at 75%；It screens of fine quality, fresh, individual big northeast tussah autumn pupa and carries out warming cocoons, gradually risen by 2d
Temperature is spare to environment temperature；
(b) field acquisition and screening of micromelalopha troglodyte pupa：Micromelalopha troglodyte pupa is acquired in nature, and to micromelalopha troglodyte parasitism
Pupa with the not parasitic pupa of micromelalopha troglodyte screen spare；Micromelalopha troglodyte parasitism pupal cell table colour-darkening, pupal cell internode elongate, and poplar is small
The not parasitic pupal cell table of boat moth is vivid；
(c) kind bee culture：Micromelalopha troglodyte parasitism pupa is put into Tube propagation, Tetrastichus septentrionalis kind bee is used as after emergence
It is spare；
(d) information substance extracts：The not parasitic pupa of micromelalopha troglodyte is impregnated with the concentration of 30/100ml with 45% alcohol
13h forms soak；
(e) northeast tussah autumn pupa is handled：By the soak sprayer containing information substance extract of step (d) to step
Suddenly the northeast tussah autumn pupa of (a) screening is uniformly sprayed, and 3.5h is placed after sprinkling, is dried spare；
(f) bee is connect：The northeast tussah autumn pupa of step (e) is put into plastic culture box, under dark condition, uses small brushes
Gently the Tetrastichus septentrionalis kind bee of step (c) is uniformly swept into box, Tetrastichus septentrionalis kind bee and northeast tussah autumn
The ratio of pupa is 40: 1, and temperature control meets bee 26h and complete in 27 DEG C, relative humidity 75%；Step (f) connects the plastic culture of bee
The length, width and height of box are respectively 15*10*7cm, uniformly open 4 radius 1cm circle air holes on upper lid, with 300 mesh Buddhist nuns on air hole
Imperial net seal；The northeast tussah autumn pupa 20 of step (e) is placed per box；Paving is one layer as possible, is no more than two layers；By the modeling
Material culture box is put into wooden numerous beehive, is put into 12 boxes per case, places the northeast tussah autumn pupa 240 of step (e) altogether；
(g) culture development：Temperature is controlled at 26 DEG C, relative humidity 75% and keeps cultivating development in the environment of ventilation；
It is Tetrastichus septentrionalis after emergence.
Above by the specific implementation mode of embodiment form, the present invention is described in detail, but this should not be managed
Solution is the specific implementation mode more than the above-mentioned subject area of the present invention is only limitted to, all skills realized based on the above of the present invention
Art all belongs to the scope of the present invention.
Claims (4)
1. a kind of artificial fecundation method of Tetrastichus septentrionalis, it is characterised in that include the following steps：
(a) Hosts are selected：It using northeast tussah autumn pupa as Hosts, is placed in freezer and stores, temperature is controlled in 4-6
DEG C, relative humidity is maintained at 70%-80%；It screens of fine quality, fresh, individual big northeast tussah autumn pupa and carries out warming cocoons, by 2d
It is spare to be gradually warming up to environment temperature；
(b) field acquisition and screening of micromelalopha troglodyte pupa：Nature acquire micromelalopha troglodyte pupa, and to micromelalopha troglodyte parasitism pupa with
The not parasitic pupa of micromelalopha troglodyte screen spare；Micromelalopha troglodyte parasitism pupal cell table colour-darkening, pupal cell internode elongate, micromelalopha troglodyte
Not parasitic pupal cell table is vivid；
(c) kind bee culture：Micromelalopha troglodyte parasitism pupa is put into Tube propagation, it is standby as Tetrastichus septentrionalis kind bee after emergence
With；
(d) information substance extracts：With the alcohol of 40%-50% with the concentration of 20-40 heads/100ml to the not parasitic pupa of micromelalopha troglodyte
10-15h is impregnated, soak is formed；
(e) northeast tussah autumn pupa is handled：By the soak sprayer containing information substance extract of step (d) to step
(a) the northeast tussah autumn pupa screened is uniformly sprayed, and 3-4h is placed after sprinkling, is dried spare；
(f) bee is connect：The northeast tussah autumn pupa of step (e) is put into plastic culture box, under dark condition, gently with small brushes
The Tetrastichus septentrionalis kind bee of step (c) is uniformly swept into box, Tetrastichus septentrionalis kind bee and northeast tussah autumn pupa
Ratio is 30-50: 1, and temperature control is at 26~28 DEG C, relative humidity 70~80%, connect bee for 24 hours~48h completes；
(g) culture development：Temperature is controlled at 23~28 DEG C, relative humidity 70%-80% and keeps cultivating in the environment of ventilation
Development；It is Tetrastichus septentrionalis after emergence.
2. the artificial fecundation method of Tetrastichus septentrionalis according to claim 1, it is characterised in that：
(a) Hosts are selected：Using northeast tussah autumn pupa as Hosts, it is placed in freezer and stores, temperature is controlled at 5 DEG C,
Relative humidity is maintained at 75%；
(d) information substance extracts：13h is impregnated to the not parasitic pupa of micromelalopha troglodyte with the concentration of 30/100ml with 45% alcohol,
Form soak；
(e) northeast tussah autumn pupa is handled：By the soak sprayer containing information substance extract of step (d) to step
(a) the northeast tussah autumn pupa screened is uniformly sprayed, and 3.5h is placed after sprinkling, is dried spare；
(f) bee is connect：The northeast tussah autumn pupa of step (e) is put into plastic culture box, under dark condition, gently with small brushes
The Tetrastichus septentrionalis kind bee of step (c) is uniformly swept into box, Tetrastichus septentrionalis kind bee and northeast tussah autumn pupa
Ratio is 40: 1, and temperature control meets bee 26h and complete in 27 DEG C, relative humidity 75%；
(g) culture development：Temperature is controlled at 26 DEG C, relative humidity 75% and keeps cultivating development in the environment of ventilation；Sprout wings
It is Tetrastichus septentrionalis afterwards.
3. the artificial fecundation method of Tetrastichus septentrionalis according to claim 1 or 2, it is characterised in that：Step (f) connects
The length, width and height of the plastic culture box of bee are respectively 15*10*7cm, and 4 radius 1cm circle air holes, air hole are uniformly opened on upper lid
Upper 300 mesh nylon wire seals；The northeast tussah autumn pupa 20 of step (e) is placed per box；Paving is one layer as possible, is no more than two
Layer.
4. the artificial fecundation method of Tetrastichus septentrionalis according to claim 3, it is characterised in that：By the plastics
Culture box is put into wooden numerous beehive, is put into 12 boxes per case, places the northeast tussah autumn pupa 240 of step (e) altogether.
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