CN105875512B - A method of fusion Pupal parasite and Verticillium lecanii prevent flies - Google Patents
A method of fusion Pupal parasite and Verticillium lecanii prevent flies Download PDFInfo
- Publication number
- CN105875512B CN105875512B CN201610218690.7A CN201610218690A CN105875512B CN 105875512 B CN105875512 B CN 105875512B CN 201610218690 A CN201610218690 A CN 201610218690A CN 105875512 B CN105875512 B CN 105875512B
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- China
- Prior art keywords
- pupa
- pupal
- flies
- fly
- parasite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M99/00—Subject matter not provided for in other groups of this subclass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
Abstract
The invention discloses a kind of methods that fusion Pupal parasite and Verticillium lecanii prevent flies, include the following steps：(1) prepare the housefly pupa 1000 of 1 2d of pupating, be placed in can；(2) prepare the Pupal parasite of 3d or so after sprouting wings；(3) spore suspension of Verticillium lecanii is prepared；(4) by Pupal parasite queen bee, access fills in the can of fly pupa；(5) the fly pupa after parasitism is placed in 25 DEG C, in 75% incubator, is incubated 5d；(6) after being removed gauze, the spore suspension of preparation in fly pupal cell table sprinkling step (3)；(7) can that fly pupa will be filled in step (6), is hung on soot or farm etc. and needs on the region wall prevented flies, from the ground height about 1m.The method that the present invention merges Pupal parasite and Verticillium lecanii prevention flies will carry out economic benefits and social benefits prevention to the pupa time of flies and adult stage, and compared with classical biological control method, effect is more preferable.
Description
Technical field
The present invention relates to insect control technology more particularly to a kind of fusion Pupal parasites and Verticillium lecanii to prevent
The method of flies.
Background technology
Flies are important Vector factors, can mechanical transmission dysentery, typhoid fever, cholera, tuberculosis, trachoma, polio,
A variety of diseases such as hepatitis and parasitic disease or pollution human foods, threaten human health, interfere the life of people.The prevention of flies is not
But the important content of preventive medicine, and the important content in highly civilized health cities and towns is built, it is Tourism at Present, creates well
Investment environment, attract foreign investment institute it is required.Currently, to the control of flies substantially based on chemical prevention, since flies are lived more
In Close-knit Habitation area and ambient enviroment, there are overlapping, food pollution caused by during prevention and human body with the behaviour area of people more
Poisoning occurs repeatedly, and flies produce resistance to many pesticides, how to carry out " innoxious control " to flies and have become
For current important scientific issues urgently to be resolved hurrily.
Invention content
The present invention is to provide a kind of fusion Pupal parasite and Verticillium lecanii prevention to solve above-mentioned deficiency
The method of flies.The present invention prevents fly pupa using Pupal parasite, and Verticillium lecanii prevents adult flies, mutually assisted by the two
Together, achieve the purpose that efficiently to prevent flies.
The above-mentioned purpose of the present invention is realized by technical solution below：A kind of fusion Pupal parasite and lecanium
The method that Verticillium dahliae prevents flies, it is characterised in that：Include the following steps：
(1) prepare the housefly pupa 1000 of 1-2d of pupating, be respectively placed in can, about 100 every bottle；
(2) Pupal parasite for preparing 3d or so after sprouting wings, fully mates, and raise with 20% hydromel；
(3) spore suspension of preparation Verticillium lecanii, a concentration of 108A/ml；
(4) by the Pupal parasite queen bee in step (2), by queen bee：Housefly pupa is 1：5 ratio accesses step
(1) in the can for filling fly pupa, bottleneck is sealed with 200 mesh gauzes, is placed in 25 DEG C, it is parasitic in 75% incubator
For 24 hours, queen bee is then removed；
(5) the fly pupa after parasitism in step (4) is placed in 25 DEG C, in 75% incubator, is incubated 5d；
(6) by after the can removal gauze in step (5), the spore prepared in fly pupal cell table sprinkling step (3) is outstanding
Supernatant liquid, until so that fly pupal cell table is moistened, for 24 hours in incubation in 25 DEG C, 75% incubator；
(7) can that fly pupa will be filled in step (6), is hung on soot or farm etc. and needs to prevent flies
Region wall on, from the ground height about 1m.
If housefly pupa is by parasitism success, from sprouting wings in pupa, the Pupal parasite can be gone in parasitic ambient enviroment
The fly pupa multiplied, if housefly pupa is not parasitic by success, the adult housefly of sprouting wings out of pupa is because body surface can carry lecanium wheel branch
The spore of bacterium brings other healthy adult flies by Verticillium lecanii parasitism or by spore, causes Verticillium lecanii in fly population
Prevalence can utilize Verticillium lecanii to prevent adult flies to achieve the effect that not only to prevent fly pupa using Pupal parasite.
Compared with the prior art, the present invention has the advantages of：The present invention merges Pupal parasite and Verticillium lecanii prevention
The method of flies will carry out economic benefits and social benefits prevention to the pupa time of flies and adult stage, and compared with classical biological control method, effect is more
It is good.
Description of the drawings
Fig. 1 is influence chart of the Pupal parasite body surface sprinkling Verticillium lecanii spore to its service life.
Fig. 2 is different time fly pupal cell table sprinkling Verticillium lecanii spore after parasitism, sprouts wings what the adult flies averagely carried
Spore count chart.
Fig. 3 is that the adult flies for sprouting wings in the housefly pupa of sprinkling Verticillium lecanii spore mix raising with healthy housefly, is made
At the cumulative mortality chart of housefly population around.
Specific implementation mode
The present invention is described in further detail with reference to embodiment.
A method of fusion Pupal parasite and Verticillium lecanii prevent flies, it is characterised in that：Including following
Step：
(1) prepare the housefly pupa 1000 of 1-2d of pupating, be respectively placed in can, about 100 every bottle；
(2) Pupal parasite for preparing 3d or so after sprouting wings, fully mates, and raise with 20% hydromel；
(3) spore suspension of preparation Verticillium lecanii, a concentration of 108A/ml；
(4) by the Pupal parasite queen bee in step (2), by queen bee：Housefly pupa is 1：5 ratio accesses step
(1) in the can for filling fly pupa, bottleneck is sealed with 200 mesh gauzes, is placed in 25 DEG C, it is parasitic in 75% incubator
For 24 hours, queen bee is then removed；
(5) the fly pupa after parasitism in step (4) is placed in 25 DEG C, in 75% incubator, is incubated 5d；
(6) by after the can removal gauze in step (5), the spore prepared in fly pupal cell table sprinkling step (3) is outstanding
Supernatant liquid, until so that fly pupal cell table is moistened, for 24 hours in incubation in 25 DEG C, 75% incubator；
(7) can that fly pupa will be filled in step (6), is hung on soot or farm etc. and needs to prevent flies
Region wall on, from the ground height about 1m.
If housefly pupa is by parasitism success, from sprouting wings in pupa, the Pupal parasite can be gone in parasitic ambient enviroment
The fly pupa multiplied, if housefly pupa is not parasitic by success, the adult housefly of sprouting wings out of pupa is because body surface can carry lecanium wheel branch
The spore of bacterium brings other healthy adult flies by Verticillium lecanii parasitism or by spore, causes Verticillium lecanii in fly population
Prevalence can utilize Verticillium lecanii to prevent adult flies to achieve the effect that not only to prevent fly pupa using Pupal parasite.
Following instance is the further explanation to the present invention.
It is being 10 by the housefly pupal cell table spray concentration of Pupal parasite parasitism 5d8The Verticillium lecanii spore of a/ml
Son in being incubated for 24 hours in 25 DEG C, 75% incubator, is subsequently placed in outdoor, finds sprinkling lecanium wheel branch until pupal cell table moistens
Bacterium spore does not make significant difference (as shown in Figure 1) to the existence of Pupal parasite, illustrates that it is peace to Pupal parasite
Complete.Meanwhile the spore count that the adult housefly body surface carries of sprouting wings in not parasitic successfully fly pupa reaches as high as 1 × 106A/
Head (as shown in Figure 2) can cause other houseflies by the way that this housefly for carrying spore is mixed raising with other healthy houseflies
Infection, caused by the death rate reach as high as 60%, median lethal time is about 13.7d (as shown in Figure 3).By the parasitic housefly of success
The Pupal parasite of sprouting wings in pupa has good control action, control effect to reach as high as 78% the fly pupa of surrounding
(as shown in the table).By merging two kinds of biological control methods, it is anti-can biology to be carried out to two kinds of worm states of flies simultaneously
It controls, control effect is more notable.
Example the above is only the implementation of the present invention is not intended to limit the scope of the invention, every to utilize this hair
Equivalent structure or equivalent flow shift made by bright specification and embodiment content, it is relevant to be applied directly or indirectly in other
Technical field is included within the scope of the present invention.
Claims (1)
1. a kind of method of fusion Pupal parasite and Verticillium lecanii prevention flies, it is characterised in that：Including following step
Suddenly：
(1) prepare the housefly pupa 1000 of 1-2d of pupating, be respectively placed in can, 100 every bottle；
(2) Pupal parasite for preparing 3d after sprouting wings, fully mates, and raise with 20% hydromel；
(3) spore suspension of preparation Verticillium lecanii, a concentration of 108A/ml；
(4) by the Pupal parasite queen bee in step (2), by queen bee：Housefly pupa is 1：5 ratio accesses step (1) institute
It states in the can for filling fly pupa, seals bottleneck with 200 mesh gauzes, be placed in incubator, parasitism for 24 hours, then removes queen bee；
(5) the fly pupa after parasitism in step (4) is placed in incubator, is incubated 5d；
(6) by after the can removal gauze in step (5), the spore suspension of preparation in step (3) is sprayed in fly pupal cell table,
Until so that fly pupal cell table is moistened, in being incubated for 24 hours in incubator；
(7) can that fly pupa will be filled in step (6), is hung on soot or farm needs the region prevented flies
On wall, height 1m from the ground.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610218690.7A CN105875512B (en) | 2016-04-08 | 2016-04-08 | A method of fusion Pupal parasite and Verticillium lecanii prevent flies |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610218690.7A CN105875512B (en) | 2016-04-08 | 2016-04-08 | A method of fusion Pupal parasite and Verticillium lecanii prevent flies |
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| Publication Number | Publication Date |
|---|---|
| CN105875512A CN105875512A (en) | 2016-08-24 |
| CN105875512B true CN105875512B (en) | 2018-10-12 |
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| CN201610218690.7A Expired - Fee Related CN105875512B (en) | 2016-04-08 | 2016-04-08 | A method of fusion Pupal parasite and Verticillium lecanii prevent flies |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1147754A (en) * | 1994-03-21 | 1997-04-16 | 佛罗里达大学 | Methods and formulations for control of insect pests |
| CN1663368A (en) * | 2004-03-03 | 2005-09-07 | 内蒙古自治区林业科学研究院 | Scleroderma guani parasitism control for loghorn beetle |
| CN104126613A (en) * | 2014-06-30 | 2014-11-05 | 泰山医学院 | Method using ants to spread verticillium lecanii for control of tree trunk stomaphissinisalicis |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3914969B2 (en) * | 2003-05-06 | 2007-05-16 | みのる産業株式会社 | Insect trap |
| JP2007054028A (en) * | 2005-08-22 | 2007-03-08 | Setsuya Yamazaki | Hygienic device for capturing and removing cockroach or the like while reducing unpleasant feeling at capturing |
-
2016
- 2016-04-08 CN CN201610218690.7A patent/CN105875512B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1147754A (en) * | 1994-03-21 | 1997-04-16 | 佛罗里达大学 | Methods and formulations for control of insect pests |
| CN1663368A (en) * | 2004-03-03 | 2005-09-07 | 内蒙古自治区林业科学研究院 | Scleroderma guani parasitism control for loghorn beetle |
| CN104126613A (en) * | 2014-06-30 | 2014-11-05 | 泰山医学院 | Method using ants to spread verticillium lecanii for control of tree trunk stomaphissinisalicis |
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| Publication number | Publication date |
|---|---|
| CN105875512A (en) | 2016-08-24 |
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