CN106718392A - A kind of method that utilization hemerobild prevents and treats greenhouse flowers insect - Google Patents
A kind of method that utilization hemerobild prevents and treats greenhouse flowers insect Download PDFInfo
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- CN106718392A CN106718392A CN201611069933.1A CN201611069933A CN106718392A CN 106718392 A CN106718392 A CN 106718392A CN 201611069933 A CN201611069933 A CN 201611069933A CN 106718392 A CN106718392 A CN 106718392A
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- hemerobild
- greenhouse
- aphid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Animal Behavior & Ethology (AREA)
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- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of method that utilization hemerobild prevents and treats greenhouse flowers insect, by the Reproduction Conditions for optimizing angle ridge hemerobild, significantly improve egg laying amount, the incubation rate of ovum, larva predatory number, the adult eclosion rate of angle ridge hemerobild, and then the effect that angle ridge hemerobild prevents and treats greenhouse flowers insect is improve, for the biological control of greenhouse flowers provides technical support.
Description
Technical field
The present invention relates to agricultural pests technical field of biological control, more particularly to one kind is using hemerobild preventing and treating greenhouse flowers evil
The method of worm.
Background technology
With the gradually expansion of flowers market demand, greenhouse has become most important, most widely used way in flower culture
Footpath.It for many flower plants while suitable growing environment is provided, and prevention and control of plant diseases, pest control problem is also adjoint.Greenhouse
Interior high temperature, high humidity, the air not enough environmental condition such as circulation result in greenhouse flowers pest and disease damage has type various, several
Amount is big, harm weight, its spread in china are fast, the anniversary frequency it is frequent the features such as, preventing and controlling is there is certain difficulty.Due to temperature
Room floriculture pest and disease damage incidence is higher, if only taking pesticide control, not only increases cost, while will also result in flowers product
Pollution.To overcome this deficiency, the biological prevention for controlling worm with worm turns into the focus of research.For example prevented and treated with Encarsia formosa
Greenhouse primary pest trialeurodes vaporariorum, the former can suppress the generation and harm of the latter, and not on flowers generation influence.And such as Chinese rose
There is serious red spider harm in cultivation, then flutter food mite to prevent and treat using another natural enemy.Purposefully Protect natural enemies, can have
Effect improves the effect of biological control.At present, measure of biotic control applies less in the greenhouse flowers prevention and control of plant diseases, pest control, but this respect
Work has the advantages that pollution-free, noresidue, effect are long, is a developing direction of following prevention and elimination of disease and pests.
Neuraopteron is used for the predominantly chrysopidae insect of biological control, and Chrysopa belongs to insect predator, widely distributed,
Species is various, there are about 1300 kinds.Chrysopa can prey on the various pests such as aphid, scale insect, red spider, leafhopper, wood louse, aleyrodid, energy
Effectively suppress the growth of pest population quantity in forest, nursery, orchard, farmland.The U.S. just utilizes early in the sixties in 20th century
Chrysopa carnea prevents and treats cotton noctuid, the factorial praluction of Chrysopa of successful implementation at present, breeding, the former Soviet Union, Britain, method
The country such as state, Canada, India also carried out the research of correlation to Chrysopa, in developed countries such as American and Britain, existing each worm state
Chrysopa is sold.Neuroptera Hemerobiidae insect is used for the report of biological control, studies less, and Hemerobiidae insect is in biological control
With very big potentiality, main cause shows three aspects：First, adult and larva predatory number are all very big, can prey on many kinds
The ovum of the insect of class, such as aphid, mite class, scale insect, wood louse and other small flexible insects etc. and adult；Second, larva
Activity is sensitive, and action is rapid, and prey ability is strong；3rd, Life of Adult is long and fertility is strong, and diapause under cryogenic is treated
Condition properly continues spawning and is bred afterwards.Neuroptera is extraordinary biological control resource, and Hemerobiidae insect is in this side
Face or blank out, inventor test during doctor by numerous studies, it is proposed that raise angle ridge hemerobild using aphid
The laboratory method of (Micromus angulatus Stephens), the method for breeding breeds angle ridge hemerobild in egg laying amount, ovum
The aspect such as incubation rate, larva predatory number, adult eclosion rate it is not ideal enough, inventor has further carried out excellent to method for breeding
Change, the characteristics of generation with reference to greenhouse flowers insect pest, it is proposed that the method that greenhouse flowers insect is prevented and treated using hemerobild, is greenhouse flowers
Biological control provide technical support.
The content of the invention
It is an object of the invention to provide a kind of method that utilization hemerobild prevents and treats greenhouse flowers insect, by soybean aphid
(Aphis glycines Matsumura) breeds angle ridge hemerobild (Micromus angulatus Stephens), then uses angle
Ridge hemerobild prevents and treats greenhouse flowers insect.
To achieve the above object, the present invention proposes following technical scheme：A kind of utilization hemerobild preventing and treating greenhouse flowers insect
Method, it is characterised in that comprise the following steps：(1) aphid is raised, the breeding of (two) hemerobild, (three) packing, (four) release hemerobild；
Preferably, the aphid is raised and comprises the following steps:
(1) big bean seedlings posting as soybean aphid (Aphis glycines Matsumura) of the selection with 2-3 piece true leaves
Main plant；
(2) the big bean seedlings with 2-3 piece true leaves are moved into aphid and breed room, every plant big bean seedlings be inoculated with 3-6 soybean aphid into
Aphid, aphid breeds indoor temperature and is maintained at 24-25 DEG C, humidity 70%-80%；
Preferably, described hemerobild breeding is comprised the following steps:
A () will wait to lay eggs in female hemerobild adult immigration raising box, then the soybean aphid that will be raised in step (1) is together with big
Bean seedlings branches and leaves are together put into raising box, and the amount that every hemerobild adult feeds soybean aphid is 30-80 heads/day；
B () female angle ridge hemerobild adult starts spawning after, the ovum that will be collected into is moved into empty raising box, works as egg hatching
Afterwards, the soybean aphid that will be raised in step (1) is together put into raising box together with big bean seedlings branches and leaves, and every hemerobild larva feeds greatly
The amount of bean aphid is 20-80 heads/day；
C () raises 8-13 days after, hemerobild larva turns into adult；
D () described step (a), (b), (c) are carried out in greenhouse, be arranged to 5 cycles, first within daily 24 hours in greenhouse
24 DEG C of individual cycle keeping temperature, humidity 45%, intensity of illumination 15Lx 2 hours, 25 DEG C of second period keeping temperature, humidity
40%th, intensity of illumination 30Lx 4 hours, the 3rd 26 DEG C of cycle keeping temperature, humidity 35%, intensity of illumination 40Lx 6 hours,
Four 24 DEG C of cycle keeping temperatures, humidity 45%, intensity of illumination 15Lx 4 hours, the 5th 23 DEG C of cycle keeping temperature, humidity
55%th, intensity of illumination 0Lx 8 hours；
Preferably, described raising box is transparent plastic box, 18-22cm long, 10-14cm wide, 6-10cm high；Lid retains
Half, in addition half cover with gauze, the aperture of the gauze is 0.05-0.15mm；
Preferably, the hemerobild is angle ridge hemerobild (Micromus angulatus Stephens)；
Preferably, hemerobild adult is dispensed using box is raised, and hemerobild larva, ovum, pupa are dispensed using plastic centrifuge tube；
Preferably, the release hemerobild comprises the following steps:The hemerobild of packing is released in greenhouse, the hemerobild with temperature
The quantity ratio of room Flower Pests is 1:40-150；
Preferably, the hemerobild of the packing is hemerobild adult, larva, pupa；
Preferably, the greenhouse flowers insect is aphid, mite, scale insect；
The beneficial effects of the invention are as follows：
1st, the Reproduction Conditions after present invention optimization, significantly improve egg laying amount, the incubation rate of ovum, the larva of angle ridge hemerobild
Predatory number, adult eclosion rate, for biological control angle ridge hemerobild provides technical support.
2nd, the angle ridge hemerobild of present invention breeding is obvious to greenhouse flowers pest controling effect, especially to aphid, mite, shell
The prevention effect of worm is good；Pollution-free, noresidue, effect are long, cost-effective.
Specific embodiment
The technical scheme to the embodiment of the present invention is described below：
Embodiment 1
1st, test method：(experiment is in triplicate)
(1) there are the big bean seedlings of 2-3 piece true leaves to move to aphid 50 disks and breeds room, every plant big bean seedlings are inoculated with 5 soybean aphids
Adult aphid, aphid breeds indoor temperature and is maintained at 24 DEG C, and humidity 75% is standby after breeding 5-7 days；
(2) the female angle ridge hemerobild adult that will wait to lay eggs moves into be raised in box, and raisings box is transparent plastic box, 20cm long,
12cm wide, 7cm high；Lid retains half, and half covers with gauze in addition, and the aperture of the gauze is 0.15mm；Again by step (1)
The soybean aphid of middle raising is together put into raising box together with a small amount of big bean seedlings branches and leaves, and the amount that every hemerobild adult feeds soybean aphid is
30-45 heads/day, prepare 30 raising boxes, and each is raised in box has 1 to wait the female angle ridge hemerobild adult that lays eggs；
(3) after female angle ridge hemerobild adult starts spawning, the ovum that will be collected into is moved into empty raising box, counts every head
The egg laying amount of female angle ridge hemerobild adult；After breeding 3.8 days, ovum starts hatching (incubation rate of statistics ovum), by step (1)
The soybean aphid of raising is together put into raising box together with big bean seedlings branches and leaves, and the amount that every hemerobild larva feeds soybean aphid is 20-70
Head/day, the daily predatory number of statistics 1-3 age hemerobild larvas；
(4) after raising 9.2 days, hemerobild larva starts to turn into adult, counts adult eclosion rate；
(5) step (2), (3), (4) are carried out in greenhouse, are arranged to 5 cycles, first within daily 24 hours in greenhouse
24 DEG C of individual cycle keeping temperature, humidity 45%, intensity of illumination 15Lx 2 hours, 25 DEG C of second period keeping temperature, humidity
40%th, intensity of illumination 30Lx 4 hours, the 3rd 26 DEG C of cycle keeping temperature, humidity 35%, intensity of illumination 40Lx 6 hours,
Four 24 DEG C of cycle keeping temperatures, humidity 45%, intensity of illumination 15Lx 4 hours, the 5th 23 DEG C of cycle keeping temperature, humidity
55%th, intensity of illumination 0Lx 8 hours；
(6) according to the quantity ratio of angle ridge hemerobild adult and aphid it is 1 in the greenhouse of plantation lily:100 separation angle lines
Arteries and veins hemerobild adult, 10 days after release, counts prevention effect in 30 days respectively；
(7) parallel control is set, and step (5) is adjusted to：" step (2), (3), (4) are carried out in greenhouse, every in greenhouse
Day is arranged to 4 cycles for 24 hours, 25.5 DEG C of a cycle keeping temperature, humidity 45%, intensity of illumination 20Lx 3 hours, the
Two 27 DEG C of cycle keeping temperatures, humidity 35%, intensity of illumination 40Lx 10 hours, the 3rd 26 DEG C of cycle keeping temperature, humidity
45%th, intensity of illumination 20Lx 3 hours, the 4th 25 DEG C of cycle keeping temperature, humidity 60%, intensity of illumination 0Lx8 hours ", so
After repeat the above steps (1), (2), (3), (4), (5), (6), obtain control 1；Step (5) is adjusted to：" step (2), (3),
(4) carried out in greenhouse, 25 DEG C of keeping temperature in greenhouse, humidity 50%, illumination in 12 hours, 12 hours dark ", then repeat
Step (1), (2), (3), (4), (5), (6) are stated, control 2 is obtained.
The difference of table 1 breeds the egg laying amount of condition inferior horn ridge hemerobild
| Breed condition | The egg laying amount (individual/day) of angle ridge hemerobild female adult pest |
| The present invention | 32.5 |
| Control 1 | 24.8 |
| Control 2 | 16.3 |
Note：The egg laying amount of angle ridge hemerobild female adult pest is all three average values of repetition experiment in table 1
The as shown by data of table 1：The optimization that the present invention breeds condition causes that the egg laying amount of angle ridge hemerobild is improved.
The difference of table 2 breeds the incubation rate of condition inferior horn ridge hemerobild ovum
| Breed condition | The incubation rate (%) of angle ridge hemerobild ovum |
| The present invention | 97.8 |
| Control 1 | 89.5 |
| Control 2 | 56.9 |
Note：The incubation rate of angle ridge hemerobild ovum is all three average values of repetition experiment in table 2
The as shown by data of table 2：The present invention breed condition optimization can significantly improve angle ridge hemerobild ovum incubation rate.
The difference of table 3 breeds the predatory number of condition inferior horn ridge hemerobild larva
| Breed condition | 1 instar larvae (head/day) | 2 instar larvaes (head/day) | 3 instar larvaes (head/day) |
| The present invention | 20 | 29 | 56 |
| Control 1 | 17 | 24 | 40 |
| Control 2 | 13 | 19 | 33 |
Note：The predatory number of angle ridge hemerobild 1-3 instar larvaes is all three average values of repetition experiment in table 3
The as shown by data of table 3：The optimization that the present invention breeds condition can improve the predatory number of angle ridge hemerobild larva.
The difference of table 4 breeds the eclosion rate of condition inferior horn ridge hemerobild
| Breed condition | Eclosion rate (%) |
| The present invention | 98.3 |
| Control 1 | 90.6 |
| Control 2 | 69.5 |
Note：The eclosion rate of angle ridge hemerobild is all three average values of repetition experiment in table 4
The as shown by data of table 4：The optimization that the present invention breeds condition can significantly improve the eclosion rate of angle ridge hemerobild.
The difference of table 5 breeds prevention effect of the condition inferior horn ridge hemerobild to aphid in greenhouse
Note：Ridge hemerobild in angle is all three average values of repetition experiment to the prevention effect of aphid in table 5
The as shown by data of table 5：The present invention breeds the optimization of condition so that preventing and treating effect of the angle ridge hemerobild to aphid in greenhouse
Fruit is improved, and prevention effect is better than prior art.
Claims (9)
1. a kind of method that utilization hemerobild prevents and treats greenhouse flowers insect, it is characterised in that comprise the following steps：（One）Aphid is raised
Support,（Two）Hemerobild breeds,（Three）Packing,（Four）Release hemerobild.
2. the method that utilization hemerobild according to claim 1 prevents and treats greenhouse flowers insect, it is characterised in that the aphid is raised
Support and comprise the following steps:
（1）Selection with 2-3 piece true leaves big bean seedlings as soybean aphid (Aphis glycines Matsumura host) plants
Thing；
（2）Big bean seedlings with 2-3 piece true leaves are moved into aphid and breeds room, every plant big bean seedlings are inoculated with 3-6 soybean aphid adult aphid, aphid
Worm breeds indoor temperature and is maintained at 24-25 DEG C, humidity 70%-80%.
3. the method that utilization hemerobild according to claim 2 prevents and treats greenhouse flowers insect, it is characterised in that described hemerobild
Breeding is comprised the following steps:
（a）The female hemerobild adult that will wait to lay eggs moves into be raised in box, then by step（One）The soybean aphid of middle raising is together with big bean seedlings
Branches and leaves are together put into raising box, and the amount that every hemerobild adult feeds soybean aphid is 30-80 heads/day；
（b）After female angle ridge hemerobild adult starts spawning, the ovum that will be collected into is moved into empty raising box, after egg hatching,
By step（One）The soybean aphid of middle raising is together put into raising box together with big bean seedlings branches and leaves, and every hemerobild larva feeds soybean aphid
Amount be 20-80 heads/day；
（c）After raising 8-13 days, hemerobild larva turns into adult；
（d）The step（a）、（b）、（c）Carried out in greenhouse, be arranged to 5 cycles, the first week within daily 24 hours in greenhouse
24 DEG C of phase keeping temperature, humidity 45%, intensity of illumination 15Lx 2 hours, 25 DEG C of second period keeping temperature, humidity 40%, illumination
Intensity 30Lx 4 hours, the 3rd 26 DEG C of cycle keeping temperature, humidity 35%, intensity of illumination 40Lx 6 hours, the 4th cycle
24 DEG C of keeping temperature, humidity 45%, intensity of illumination 15Lx 4 hours, the 5th 23 DEG C of cycle keeping temperature, humidity 55%, illumination are strong
Degree 0Lx 8 hours.
4. the method that utilization hemerobild according to claim 3 prevents and treats greenhouse flowers insect, it is characterised in that described raising
Box is transparent plastic box, 18-22cm long, 10-14cm wide, 6-10cm high；Lid retains half, and half covers with gauze in addition, institute
The aperture for stating gauze is 0.05-0.15mm.
5. the method that utilization hemerobild according to claim 4 prevents and treats greenhouse flowers insect, it is characterised in that the hemerobild is
Angle ridge hemerobild (Micromus angulatus Stephens)。
6. the method that utilization hemerobild according to claim 5 prevents and treats greenhouse flowers insect, it is characterised in that the packing bag
Include following steps:Hemerobild adult is dispensed using box is raised, and hemerobild larva, ovum, pupa are dispensed using plastic centrifuge tube.
7. the method that utilization hemerobild according to claim 6 prevents and treats greenhouse flowers insect, it is characterised in that the release is brown
Sandfly comprises the following steps:The hemerobild of packing is released in greenhouse, the hemerobild is 1 with the quantity ratio of greenhouse flowers insect:
40-150。
8. the method that utilization hemerobild according to claim 7 prevents and treats greenhouse flowers insect, it is characterised in that the packing
Hemerobild is hemerobild adult, larva, pupa.
9. the method that utilization hemerobild according to claim 8 prevents and treats greenhouse flowers insect, it is characterised in that the greenhouse flower
Grass insect is aphid, mite, scale insect.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611069933.1A CN106718392A (en) | 2016-11-29 | 2016-11-29 | A kind of method that utilization hemerobild prevents and treats greenhouse flowers insect |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611069933.1A CN106718392A (en) | 2016-11-29 | 2016-11-29 | A kind of method that utilization hemerobild prevents and treats greenhouse flowers insect |
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| CN201611069933.1A Pending CN106718392A (en) | 2016-11-29 | 2016-11-29 | A kind of method that utilization hemerobild prevents and treats greenhouse flowers insect |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108967751A (en) * | 2018-09-26 | 2018-12-11 | 江苏丘陵地区南京农业科学研究所 | A kind of man-made feeds for breeding hemerobild |
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| CN108967751A (en) * | 2018-09-26 | 2018-12-11 | 江苏丘陵地区南京农业科学研究所 | A kind of man-made feeds for breeding hemerobild |
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Application publication date: 20170531 |