CN112075444A - Sex attractant for Spodoptera litura and application thereof - Google Patents
Sex attractant for Spodoptera litura and application thereof Download PDFInfo
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- CN112075444A CN112075444A CN201910518719.7A CN201910518719A CN112075444A CN 112075444 A CN112075444 A CN 112075444A CN 201910518719 A CN201910518719 A CN 201910518719A CN 112075444 A CN112075444 A CN 112075444A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/10—Catching insects by using Traps
- A01M1/106—Catching insects by using Traps for flying insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Insects & Arthropods (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a sex attractant for Trichoplusia ni and application thereof, belonging to the technical field of insect sex pheromone preparation, wherein the sex attractant comprises cis-7-dodecene acetate, cis-11-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate, and the mass ratio of the cis-7-dodecene acetate to the cis-11-hexadecene-1-ol to the cis-9-tetradecene-3-methyl-2-butenoate is (2.5-3.5): (1.5-2.6): (0.5 to 1.5). The sex attractant for the Trichoplusia ni has the advantages of simple preparation, convenient use, strong trapping capability and good stability; strong specificity, no resistance, no harm to natural enemies, no toxicity to people and livestock and environmental protection; the duration is long, and the control effect can be maintained for more than 30 days. The sex attractant for the broad shin armyworm can be applied to the insect condition monitoring, the mass trapping and the interference mating of the broad shin armyworm, and has important significance for comprehensively controlling the broad shin armyworm.
Description
Technical Field
The invention relates to the technical field of insect sex pheromone preparation, in particular to a sex attractant for panopneumonia armyworm and a preparation method and application thereof.
Background
Trichoplusia scoparia (Schinia scitosa) belongs to Lepidoptera, the family of Noctuidae, and is an important agricultural pest. Widely distributed in east, south, middle, Europe and North America of Asia, and mainly distributed in North Hebei, Shandong, Liaoning, inner Mongolia and Jiangsu. The host plant of Trichoplusia ni comprises leguminous plant, Artemisia plant and Chenopodium plant, and is mainly harmed by feeding larva. Like other phytophagous noctuidae insects, the insect has a predatory property, can cause great damage to crops, and in northern Xinjiang, the damage degree of the broad-shin looper is far higher than that of cotton bollworms, and the broad-shin looper causes great economic loss to quinoa in the Qinghai in 2017. The worm has strong reproductive capacity, and the egg laying amount of a single female can reach thousands of eggs. The imago comes out in the daytime and at night, has strong concealment and phototaxis and has good tropism to the sugar vinegar liquid. The light trapping and the sugar-vinegar liquid trapping can be adopted, but the black light lamp and the sugar-vinegar liquid have limitation in prevention and control because of the limitation of environmental conditions. Chemical control is mostly adopted in the prior art, but a great amount of chemical pesticides can not only cause the drug resistance of the broad shin armyworm, but also cause worry about the safety of crops, so agricultural scientists gradually change the chemical control into biological control.
The new technology of biological pest control by using the physiological and biochemical characteristics and the behavioral characteristics of insects is increasingly paid attention by agricultural scientists. Insect pheromones are intraspecific hormones secreted from various glands of insects and having different actions, and are chemical substances for exchanging information between the same kind of individuals and other kinds of individuals. According to the principle that sex pheromone released by female insects has strong attraction capacity on the same kind of male insects, the components and the proportion of the sex attractant for the female Trichoplusia ni can be accurately applied to insect condition prediction, mating interference and mass trapping, and the ideal effect of comprehensively treating the Trichoplusia ni is achieved.
Disclosure of Invention
In view of this, the present invention aims to provide a sex attractant for broad shin armyworm and an application thereof, so as to improve the effect of comprehensive treatment of the broad shin armyworm.
In order to achieve the purpose, the following technical scheme is adopted:
the sex attractant for the broad-shin noctuid is characterized by comprising one or more of cis-7-dodecene acetate, cis-11-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate.
Optionally, the sex attractant for the broad shin moth is cis-7-dodecene acetate, cis-11-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate, and the mass ratio is (2.5-3.5): (1.5-2.6): (0.5 to 1.5).
Preferably, the sex attractant for the broad shin moth is cis-7-dodecene acetate, cis-11-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate, and the mass ratio is (2.6-3.1): (1.7-2.2): (0.8 to 1.2).
Preferably, the sex attractant for the broad shin moth is cis-7-dodecene acetate, cis-11-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate, and the mass ratio of the cis-7-dodecene acetate to the cis-9-tetradecene-3-methyl-2-butenoate is 3:2: 1.
the wide-shank noctuid sex attractant can be made into a sex attractant attracting core, and the trapper fixed with the wide-shank noctuid sex attractant attracting core is hung in an area where the wide-shank noctuid can or can occur and is used for monitoring the pest situation of the wide-shank noctuid, largely trapping and interfering mating.
The preparation method of the sex attractant lure comprises the following steps: dripping n-hexane solution containing the sex attractant for the Spodoptera cuneata on the lure core (namely on the reversed rubber head), dripping 100 mu L of n-hexane on the lure core after the n-hexane solution containing the sex attractant for the Spodoptera cuneata volatilizes, preparing the lure core containing the sex attractant for the Spodoptera cuneata after the n-hexane volatilizes, filling the prepared lure core into aluminum foil, sealing, and refrigerating at-20 ℃.
The trap is one of a sticky trap, a boat-shaped trap, a triangular trap, a barrel-shaped trap and a basin-shaped trap.
Compared with the prior art, the invention has the following beneficial effects: the sex attractant for the Trichoplusia ni is simple to prepare and easy to obtain raw materials; has strong trapping ability, long lasting period and prevention and control effect lasting for more than 30 days; strong specificity, and does not cause the resistance of the broad shank looper; is harmless to human and livestock and environment-friendly; strong stress resistance and convenient use. The invention can be accurately applied to insect condition prediction, mating interference and mass trapping, and has important significance for comprehensively treating the Spodoptera cuneata.
Drawings
Figure 1 is a graph of mean male trap number for different lure formulations, where different letters indicate male trap number at the 0.05 level.
Figure 2 is a graph of the average number of males trapped at different lure hang heights, with different letters indicating the number of males trapped at the 0.05 level.
Detailed Description
The present invention is described in detail below with reference to the drawings and examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1 screening of optimal formulation of Trichoplusia ni attractant
Dripping n-hexane solution containing the sex attractant for the broad shin moth onto the lure core, dripping 100 mu L of n-hexane onto the lure core after the n-hexane solution containing the sex attractant for the broad shin moth volatilizes, and obtaining the lure core containing the sex attractant for the broad shin moth after the n-hexane volatilizes. The lure was placed in a box trap and then the trap was placed in a plot where broad shin moth development was more severe. In order to ensure the release effect, 200-2400 microgram of the sex attractant for the broad shin moth is placed in each 1mL of rubber lure core, the average distance between traps is 10m, the height of the lure core is set to be 100cm from the ground, and the trap type is a ship-shaped trap. To further illustrate the feasibility of this example, 10 specific formulations are given in Table 1
TABLE 1
The number of the wide shank moths caught at one time after the placement was investigated every two days, the trapping positions were randomly changed, the investigation was continued for 30 days, and the investigation results are shown in table 2:
TABLE 2
The data in the table are counted and made into figure 1, and as can be seen from figure 1, the sex attractant for the broad shin moth has a good trapping effect on the broad shin moth. As shown by comparison of the significance of the difference (P < 0.05), the trapping effect of the formula 9 (with the addition of the cis-7-dodecene acetate, the cis-hexadecene-1-ol and the cis-9-tetradecene-3-methyl-2-butenoate in the dosages of 600. mu.g, 400. mu.g and 200. mu.g, respectively) is the best. The maximum trapping number of the male insects is shown when the mass ratio of cis-7-dodecene acetate, cis-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate is 3:2:1 and the amount of the core inducing additive is 1200 mu g.
Example 2 Wide shank moth sex attractant field screening trap optimum height
The lure core was prepared using the formulation 9 in example 1, a soybean field in the area of Yanqing with a large number of Trichoplusia ni was selected, the area was more than 10 mu, the distance between traps was about 10m, the above-mentioned box-type traps containing Trichoplusia ni sex attractant were randomly placed, and 3 replicates were set for each treatment. Different trap heights are provided, and to further illustrate the feasibility of this embodiment, 10 different trap heights are given in table 3.
TABLE 3
The number of the caught Trichoplusia ni was investigated every two days after the placement, the trapping positions were randomly changed, the investigation was continued for 30 days, and the investigation results are shown in Table 4:
TABLE 4
The data in table 4 are counted and shown in fig. 2, and it can be seen from fig. 2 that the trapping effect of the sex attractant for noctuid is in a positive relationship with the hanging height when the lure is placed at a height of 0.2 to 1.0 m. When the hanging height is 1.0m, the number of the induced male caterpillars of the broad shin armyworm is the largest, and the effect is the best. When the number of traps decreases with increasing suspension height. The male looper has weak flight ability, and is preferred to search mating female insects in low-altitude flight.
The above disclosure is only for the specific embodiment of the present invention, but the present invention is not limited thereto, and any variations that can be made by those skilled in the art should fall within the scope of the present invention.
Claims (7)
1. The sex attractant for the broad-shin noctuid is characterized by comprising one or more of cis-7-dodecene acetate, cis-11-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate.
2. The sex attractant for the wide shank moth according to claim 1, wherein the sex attractant for the wide shank moth is cis-7-dodecene acetate, cis-11-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate, and the mass ratio is (2.5-3.5): (1.5-2.6): (0.5 to 1.5).
3. The sex attractant for the wide shank moth according to claim 1, wherein the sex attractant for the wide shank moth is cis-7-dodecene acetate, cis-11-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate, and the mass ratio is (2.6-3.1): (1.7-2.2): (0.8 to 1.2).
4. The sex attractant for the wide shank moth according to claim 1, wherein the sex attractant for the wide shank moth is cis-7-dodecene acetate, cis-11-hexadecene-1-ol and cis-9-tetradecene-3-methyl-2-butenoate, and the mass ratio of the cis-7-dodecene acetate to the cis-11-hexadecene-1-ol to the cis-9-tetradecene-3-methyl-2-butenoate is 3:2: 1.
5. use of a sex attractant for the wide shank moth according to claim 1, characterized in that the sex attractant lure is made such that the trap to which the sex attractant lure for the wide shank moth is fixed is suspended in the area where the wide shank moth occurs or is likely to occur, for the purpose of wide shank moth pest situation monitoring, mass trapping and mating disruption.
6. The use of the sex attractant for Spodoptera cunea according to claim 5, wherein the sex attractant lure is prepared by the following steps: dripping n-hexane solution containing the sex attractant for the Spodoptera cuneata on the lure core, dripping 100 mu L of n-hexane on the lure core after the n-hexane solution containing the sex attractant for the Spodoptera cuneata is volatilized, preparing the lure core containing the sex attractant for the Spodoptera cuneata after the n-hexane is volatilized, filling the prepared lure core into an aluminum foil, sealing, and refrigerating at-20 ℃.
7. The use of a sex attractant for broad shin armyworm according to claim 5, wherein said trap is one of a mythimna separata trap, a boat trap, a triangular trap, a bucket trap and a basin trap.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910518719.7A CN112075444A (en) | 2019-06-15 | 2019-06-15 | Sex attractant for Spodoptera litura and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910518719.7A CN112075444A (en) | 2019-06-15 | 2019-06-15 | Sex attractant for Spodoptera litura and application thereof |
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| CN112075444A true CN112075444A (en) | 2020-12-15 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112021310A (en) * | 2019-06-03 | 2020-12-04 | 中国农业科学院植物保护研究所 | Sex pheromone composition for preventing and treating panopneumonia armyworm |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467020A (en) * | 2017-08-20 | 2017-12-15 | 中国农业科学院植物保护研究所 | A kind of lure composition for being used to prevent and treat black cutworm |
| CN112021310A (en) * | 2019-06-03 | 2020-12-04 | 中国农业科学院植物保护研究所 | Sex pheromone composition for preventing and treating panopneumonia armyworm |
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- 2019-06-15 CN CN201910518719.7A patent/CN112075444A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467020A (en) * | 2017-08-20 | 2017-12-15 | 中国农业科学院植物保护研究所 | A kind of lure composition for being used to prevent and treat black cutworm |
| CN112021310A (en) * | 2019-06-03 | 2020-12-04 | 中国农业科学院植物保护研究所 | Sex pheromone composition for preventing and treating panopneumonia armyworm |
Non-Patent Citations (1)
| Title |
|---|
| TETSU ANDO等: "Sex Attractants for Male Lepidoptera", 《AGRIC.BIOL.CHEM.》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112021310A (en) * | 2019-06-03 | 2020-12-04 | 中国农业科学院植物保护研究所 | Sex pheromone composition for preventing and treating panopneumonia armyworm |
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Application publication date: 20201215 |