CN115997781B - Special attractant for preventing and controlling lesser leafhoppers in tea garden - Google Patents
Special attractant for preventing and controlling lesser leafhoppers in tea garden Download PDFInfo
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- 241001414720 Cicadellidae Species 0.000 title claims abstract description 59
- 239000005667 attractant Substances 0.000 title claims abstract description 28
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 22
- 241001122767 Theaceae Species 0.000 title claims abstract 8
- GLZPCOQZEFWAFX-UHFFFAOYSA-N Geraniol Chemical compound CC(C)=CCCC(C)=CCO GLZPCOQZEFWAFX-UHFFFAOYSA-N 0.000 claims abstract description 38
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 claims abstract description 38
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 claims abstract description 19
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 claims abstract description 19
- GLZPCOQZEFWAFX-YFHOEESVSA-N Geraniol Natural products CC(C)=CCC\C(C)=C/CO GLZPCOQZEFWAFX-YFHOEESVSA-N 0.000 claims abstract description 19
- 239000005792 Geraniol Substances 0.000 claims abstract description 19
- 229940113087 geraniol Drugs 0.000 claims abstract description 19
- 229930007744 linalool Natural products 0.000 claims abstract description 19
- 230000002265 prevention Effects 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 14
- 239000002904 solvent Substances 0.000 claims description 12
- DOOTYTYQINUNNV-UHFFFAOYSA-N Triethyl citrate Chemical group CCOC(=O)CC(O)(C(=O)OCC)CC(=O)OCC DOOTYTYQINUNNV-UHFFFAOYSA-N 0.000 claims description 7
- VMYFZRTXGLUXMZ-UHFFFAOYSA-N triethyl citrate Natural products CCOC(=O)C(O)(C(=O)OCC)C(=O)OCC VMYFZRTXGLUXMZ-UHFFFAOYSA-N 0.000 claims description 7
- 235000013769 triethyl citrate Nutrition 0.000 claims description 7
- 239000001069 triethyl citrate Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 2
- 239000000877 Sex Attractant Substances 0.000 claims 2
- 239000004480 active ingredient Substances 0.000 claims 2
- 241000607479 Yersinia pestis Species 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 8
- 239000000126 substance Substances 0.000 abstract description 6
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- 238000012544 monitoring process Methods 0.000 abstract 1
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- 235000006468 Thea sinensis Nutrition 0.000 description 18
- 235000009569 green tea Nutrition 0.000 description 15
- 235000020279 black tea Nutrition 0.000 description 14
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 12
- 240000003553 Leptospermum scoparium Species 0.000 description 12
- 235000015459 Lycium barbarum Nutrition 0.000 description 12
- 235000020334 white tea Nutrition 0.000 description 12
- 241000238631 Hexapoda Species 0.000 description 10
- 239000003039 volatile agent Substances 0.000 description 10
- 238000011282 treatment Methods 0.000 description 9
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
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- 235000016299 Canarium odontophyllum Nutrition 0.000 description 1
- 244000001582 Canarium odontophyllum Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000995023 Empoasca Species 0.000 description 1
- 241000086608 Empoasca vitis Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 239000005906 Imidacloprid Substances 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 244000170916 Paeonia officinalis Species 0.000 description 1
- 235000006484 Paeonia officinalis Nutrition 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 239000005941 Thiamethoxam Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 239000012159 carrier gas Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
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- 239000000428 dust Substances 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 description 1
- 229940056881 imidacloprid Drugs 0.000 description 1
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- 238000004949 mass spectrometry Methods 0.000 description 1
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- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
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- 239000003415 peat Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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- NWWZPOKUUAIXIW-FLIBITNWSA-N thiamethoxam Chemical compound [O-][N+](=O)\N=C/1N(C)COCN\1CC1=CN=C(Cl)S1 NWWZPOKUUAIXIW-FLIBITNWSA-N 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a special attractant for preventing and controlling leafhoppers in a tea garden, which relates to the technical field of pest control and comprises the following main components: hexanol, linalool, and geraniol in a mass ratio of 6:16:12 or 8:12:12. The special attractant has good attracting effect on the lesser leafhoppers, and provides support for the establishment of other effective prevention and control measures and the reduction of the usage amount of chemical pesticides, thereby realizing the accurate monitoring and the green and high-efficiency prevention and control of the lesser leafhoppers.
Description
Technical Field
The invention relates to the technical field of pest control, in particular to a special attractant for controlling leafhoppers in a tea garden and application thereof.
Background
The Fujian tea is a national tea province, is also a multi-tea-producing area, has a tea history and tea culture of over one thousand years, is rich and complete in tea varieties, has a strong tea production capacity due to the wide region of the tea-producing area, and is a main tea production and export area in China for many years. Fujian tea leaves take an extremely important role in the world, and are out of the names of Tieguanyin, dahongpao, wuyi rock tea, pekoe silver needle, congou black tea and the like. Tea is a traditional product for export of agricultural economic crops in Fujian province, the export amount of the tea is more than ten millions of dollars in recent years, and the export quantity and the export amount of the tea are all 5 nationally, so that the tea has strong international market development capability and plays a very important role in increasing income of peasants and creating foreign exchange in export. The tea lesser leafhoppers are the main pests for planting the tea in the province. The insect is commonly known as floating dust seed, she Tiaochong and the like in the family of the leafhopper of the order hemiptera, and occurs in tea provinces and autonomous areas all over the country; sucking tender tip juice of tea tree with adult and nymph to consume nutrients and water; female insects lay eggs in tender shoot tissues, and the growth of buds is blocked. To cause the damaged buds She Sheyuan to turn yellow and dry and burnt after damage, the veins become red, the growth stagnates and hardens, even fall off, the damaged buds and leaves are fragile, astringent and bad in quality. The insects occur for about 8-12 generations in one year, and alternate in generation. The tea is seriously damaged in summer and autumn, the tea tree buds She Quansu, hardening, leaf tips and leaf edges are scorched and scorched, the growth of the bud tips is slow, and the influence on the yield and quality of the tea is great. The insect pest is small in size, more in generation and easy to generate drug resistance, the prevention difficulty is high, the insect pest is used for preventing and controlling tea leafhoppers by thousands of tons each year, on the selection of the types of the insect pest, systemic insect pests such as imidacloprid, thiamethoxam and the like are mainly used for preventing and controlling due to the fact that the leafhoppers belong to piercing-sucking mouthpart insect pests, the ecological environment is not good, and more green and effective measures are needed for cooperative prevention and control.
Plant volatile organic compounds (Volatileorganiccompounds, VOCs) are important signal substances of pest localization hosts, and tea leaves are no exception, and the VOCs are necessarily important signal substances of tea lesser leafhoppers localization hosts; since plant volatiles are naturally derived, relatively safe, and do not produce residues and resistance to drugs, the development of pest attractants using plant volatiles has become an important research direction for pest management. Therefore, the method is urgently needed to excavate volatile substances capable of attracting the tea leafhoppers, is applied to the development of attractants, is used for guiding the selection and the use of pesticides, is beneficial to reducing the use amount of the pesticides and the invasion of pests, has very obvious economic and ecological effects, and has very broad application prospects.
Therefore, how to provide a specific attractant for preventing and controlling leafhoppers in tea gardens is a problem to be solved by the person skilled in the art.
Disclosure of Invention
In view of the above, the invention provides a special attractant for preventing and controlling leafhoppers in tea gardens, which has good attracting effect on the leafhoppers.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
A special attractant for preventing and controlling tea garden lesser leafhoppers comprises the following main components: hexanol, linalool, and geraniol, and the mass ratio of the hexanol, linalool, and geraniol is 6:16:12 or 8:12:12.
As a preferable technical scheme of the invention, the mass ratio of the hexanol to the linalool to the geraniol is 6:16:12.
As a preferable technical scheme of the invention, the attractant also comprises a slow-release solvent, and the weight ratio of the main component to the slow-release solvent is 32:68.
More preferably, the slow release solvent is triethyl citrate.
The invention also provides a preparation method of the special attractant for preventing and controlling tea garden leafhoppers, which comprises the following steps: and weighing the hexanol, the linalool and the geraniol according to the mass ratio, and uniformly mixing.
As a preferred embodiment of the present invention, the method further comprises dissolving the mixture of hexanol, linalool and geraniol in a slow release solvent.
More preferably, the slow release solvent is triethyl citrate.
It is a further object of the present invention to provide the use of any of the above-described proprietary attractants or products prepared by any of the above-described methods for controlling leafhoppers.
Compared with the prior art, the special attractant for the lesser leafhoppers is provided, is special for attracting lesser leafhoppers in tea gardens, has a good attracting effect, is safe and free of side effects, and reduces quality damage of lesser leafhoppers to tea gardens.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 is a graph showing the result of the attraction of different tea trees to leafhoppers according to example 1 of the present invention;
fig. 2 is a diagram showing the result of attracting with different proportions of the attractant provided in example 3 of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Effect of different tea plant volatiles on the trapping amount of Empoasca minor
Materials and methods
(1) Test plants: 3 pot plants of green tea (Jiulong green buds), black tea (golden peony) and white tea (Jiulong Dabai) with average plant height of 20-25cm are taken, and are transplanted into a seedling pot by peat soil, and the growth is recovered for 10d for standby. Leafhopper): tea leafhoppers are collected from a tea tree planting base, and are bred by tea leaves in an insect breeding cage under the conditions of 24-27 ℃ at the room temperature, 55-65% relative humidity and 16L/8D photoperiod, and adults with 2d eclosion in the next generation are taken out and starved for 12 hours for standby.
(2) The test method comprises the following steps: under the conditions of 24-27 ℃ and darkness and sealing, the trapping amount of 3 tea tree volatiles to the tea leafhoppers is measured and analyzed by adopting a Y-type olfactometer system with two arms connected with a glass gas collecting tank (with the height of 35cm and the diameter of 25 cm). Test 6 groups of treatments: ① Green tea vs air, ② black tea vs air, ③ white tea vs air, ④ green tea vs black tea, ⑤ green tea vs white tea, ⑥ black tea vs white tea; 1 plant of each treated tea tree is repeated for 3 times; each group of treatment observation is carried out on 30 tea leafhoppers, and the effective selection is counted by the fact that the tea leafhoppers climb to more than 1/3 of two arms of a Y-type olfactometer for more than 30 seconds within 5 minutes; each tea lesser leafhopper is only used for 1 time in the test, and each observation of 5 times is replaced by 1 time of Y-shaped olfactometer; the differences in the amount of tea leafhoppers induced in each treatment were compared by t-test.
Results and analysis
The trapping amount of 3 tea tree volatiles to the tea leafhoppers is obviously different; the average trapping amount of green tea to tea leafhoppers is the greatest, and the green tea vs air, green tea vs black tea and green tea vs white tea respectively reach 23.67, 18.67 and 16.33 heads, which are obviously more than air, black tea and white tea; the average trapping quantity of the white tea to the tea leafhoppers is second, and the white tea vs air, the black tea vs white tea and the green tea vs white tea are respectively 19.67, 14.67 and 12.33 heads, which are obviously more than the air and the black tea; the average trapping amount of black tea to tea leafhoppers is minimal, and only 16.67, 10.33 and 10.00 heads are respectively used for black tea vs air, green tea vs black tea and black tea vs white tea, and only the air is significantly more than the air, see figure 1. It can be seen that the 3 volatile matters of tea trees induce the tea leafhoppers most, the white tea is located next to the green tea and the black tea is the least.
Example 2
Relationship between volatile content of tea tree and the amount of tea leafhopper
Materials and methods
3 Tea plants (namely 3 times of repetition) are taken, all leaves of each tea plant are rapidly cut, and are immediately put into liquid nitrogen for fixation for 15min, so that the generation of wound-induced volatile matters is prevented; extracting and detecting 3 volatile matters of tea trees by adopting a solid-phase microextraction method and GC-MS. GC-MS chromatographic column: HP-5MS capillary column (30 m x 0.25mm x 0.25 um); the temperature of the sample inlet is 280 ℃, the temperature of the ion source is 280 ℃, and the temperature of the quaternary rod is 280 ℃; programming temperature: the initial temperature is kept at 40 ℃ for 5min, then the temperature is increased to 100 ℃ at a speed of 3.5 ℃ for -1 min, then the temperature is increased to 200 ℃ at a speed of 8.0 ℃ for -1 and the temperature is kept for 5min, and then the temperature is increased to 280 ℃ at a speed of 150 ℃ for -1 and the temperature is kept for 15min; mass spectrometry conditions: the ionization mode is EI, the electron energy is 70eV, the carrier gas is 99.9995% helium, and the mass range is 50-500amu. The correlation of the relative amounts of volatiles to the amount of green leafhopper attraction was analyzed using Pearson correlation.
Results and analysis
The relative amounts of the volatiles were significantly different among the 3 tea trees, with the relative amounts of hexanol, linalool and geraniol all showing a significant positive correlation (r.gtoreq.0.9976, p < 0.05) with the amount of tea leafhoppers trapped (Table 1). Therefore, the 3 volatile matters have a larger influence on the tea lesser leafhopper trapping amount, and are key volatile matters for attracting tea lesser leafhoppers by tea trees.
TABLE 1 relationship between tea volatile content and tea Empoasca tea
Example 3
Tea volatile standard for attracting tea leafhoppers
Materials and methods
Based on examples 1 and 2, standards of hexanol, linalool and geraniol (purity 97-99%) were purchased, each volatiles was first diluted to different concentrations with the slow release solvent triethyl citrate, where 2,4 and 6 μg/100 μl of hexanol, 4, 8 and 12 μg/100 μl of linalool, 3, 6 and 9 μg/100 μl of geraniol, and air was used as a blank for each treatment. Then respectively sucking 1mL of suspension solution with each treatment concentration of each single volatile substance, dripping the suspension solution on 1 piece of small cotton, placing the small cotton in an open glass culture dish, and placing the culture dish in a gas collection tank; subsequently, according to the method of example 1, the trapping amount of each treatment on the tea leafhoppers is observed by adopting a Y-type olfactometer system; each group of treatments was observed for 30 tea leafhoppers and repeated 3 times. The differences in the amount of tea leafhoppers induced in each treatment were then compared by t-test.
Results and analysis
The trapping amount of 3 volatiles to the tea leafhoppers generally increases with increasing concentration; in hexanol, the trapping amounts of 2 μg/100 μl and 4 μg/100 μl were significantly higher than the control, and the trapping amounts of 6 μg/100 μl were significantly higher than the control; in linalool, the trapping amount of 4 μg/100 μl was significantly higher than that of the control, and the trapping amounts of 8 μg/100 μl and 12 μg/100 μl were significantly higher than that of the control; in geraniol, the trapping amounts were all significantly higher for the 3 concentrations than for the control (table 2). Therefore, 3 volatile matters can effectively attract the tea leafhoppers.
TABLE 2 attraction of tea plant volatile products to tea leafhoppers
Example 4
Influence of volatiles with different proportions on trapping amount of tea leafhoppers
Materials and methods
Based on examples 1,2 and 3, 2 concentrations of each of the hexanol, linalool and geraniol standard substances were set (table 3), 4 ratios (table 4) were designed by using the orthogonal table L 4(23, and the respective solutions were dissolved in the slow-release solvent triethyl citrate to prepare the attractants (the ratio of the main component to the triethyl citrate was 32:68). Respectively sucking 1mL of each attractant, dripping the attractants on 1 piece of small cotton, then placing the small cotton in an open glass culture dish, placing the culture dish in a gas collection tank, and using green tea with the greatest attraction amount of the tea small leafhoppers as a control in the other gas collection tank for simulating a field scene; according to the method of example 1, the amount of attraction of each attractant to tea leafhoppers was observed using a Y-type olfactometer system; each group of treatments was observed for 30 tea leafhoppers and repeated 3 times. The differences in the amount of tea leafhoppers trapped in each group were compared by t-test.
Table 33 design of the level concentration of volatile 2 (μg/100 μl)
| Factor level | Hexanol | Linalool | Geraniol |
| 1 | 6 | 12 | 9 |
| 2 | 8 | 16 | 12 |
TABLE 4 orthogonal design match of volatile concentration L 4(23 (μg/100 μl)
| Volatile matter proportioning | Hexanol | Linalool | Geraniol |
| 1 | 6 | 12 | 9 |
| 2 | 6 | 16 | 12 |
| 3 | 8 | 12 | 12 |
| 4 | 8 | 16 | 9 |
Results and analysis
The 4 proportion attractants have obviously different attraction amounts to the tea leafhoppers. The attractant with the proportion of 2 and 3 has higher trapping quantity which respectively reaches 21.33 heads and 18.67 heads and is also extremely obvious and obviously higher than green tea; the trapping amounts of the mixture ratios 1 and 4 were slightly higher than that of green tea, but the differences from green tea were not significant (see fig. 2). The comprehensive evaluation of the attracting effect of each proportion shows that the mass ratio of the hexanol to the linalool to the geraniol is 6:16:12 and 8:12:12, and the 2 preferential proportions are suitable proportions for preparing the tea lesser leafhopper special attractant, have a synergistic effect relative to a single volatile, can efficiently attract the insects, are used for guiding the selection and use of pesticides, are beneficial to reducing the dosage of the pesticides and the invasion of the pests, and have very obvious economic and ecological effects, thus having very broad application prospects.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. The special attractant for preventing and controlling tea garden lesser leafhoppers is characterized in that the active ingredients of the attractant are hexanol, linalool and geraniol, and the mass ratio of the hexanol to the linalool to the geraniol is 6:16:12 or 8:12:12.
2. A tea garden leafhopper prevention and control specific attractant as claimed in claim 1, wherein the mass ratio of hexanol, linalool and geraniol is 6:16:12.
3. A sex attractant for controlling leafhoppers in tea gardens as claimed in claim 1, further comprising a slow release solvent, wherein the weight ratio of active ingredient to slow release solvent is 32:68.
4. A tea garden leafhopper prevention and control sex attractant as claimed in claim 3, wherein the slow release solvent is triethyl citrate.
5. The method for preparing the special attractant for preventing and controlling leafhoppers in tea gardens as claimed in any one of claims 1 to 4, which is characterized by comprising the following steps: and weighing the hexanol, the linalool and the geraniol according to the mass ratio, and uniformly mixing.
6. The method of claim 5, further comprising dissolving the mixture of hexanol, linalool and geraniol in a slow release solvent.
7. The method for preparing a specific attractant for preventing and controlling tea garden leafhoppers according to claim 6, wherein the slow-release solvent is triethyl citrate.
8. Use of the specific attractant according to any one of claims 1 to 4 or the product prepared by the method according to any one of claims 5 to 7 for controlling leafhoppers.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102187852A (en) * | 2011-05-24 | 2011-09-21 | 中国计量学院 | Method for preventing and controlling empoasca vitis by trapping |
| CN103651305A (en) * | 2013-12-22 | 2014-03-26 | 中国计量学院 | Method for trapping empoasca vitis gothe by means of tea plantation weed rosemary smell |
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- 2022-12-16 CN CN202211626627.9A patent/CN115997781B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102187852A (en) * | 2011-05-24 | 2011-09-21 | 中国计量学院 | Method for preventing and controlling empoasca vitis by trapping |
| CN103651305A (en) * | 2013-12-22 | 2014-03-26 | 中国计量学院 | Method for trapping empoasca vitis gothe by means of tea plantation weed rosemary smell |
Non-Patent Citations (2)
| Title |
|---|
| Dan Mu等.Behavioral responses for evaluating the attractiveness of specific tea shoot volatiles to the tea green leafhopper,Empoaca vitis.Insect Science.2012,第19卷229–238. * |
| 陈宗懋,许宁,韩宝瑜,赵冬香.茶树-害虫-天敌间的化学信息联系.茶叶科学.2003,2338-45. * |
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