CN103651305A - Method for trapping empoasca vitis gothe by means of tea plantation weed rosemary smell - Google Patents
Method for trapping empoasca vitis gothe by means of tea plantation weed rosemary smell Download PDFInfo
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- CN103651305A CN103651305A CN201310714786.9A CN201310714786A CN103651305A CN 103651305 A CN103651305 A CN 103651305A CN 201310714786 A CN201310714786 A CN 201310714786A CN 103651305 A CN103651305 A CN 103651305A
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Abstract
The invention provides a method for trapping empoasca vitis gothe by means of tea plantation weed rosemary smell. The rosemary smell is used as a smell source, five kinds of components of the rosemary smell are mixed to prepare an empoasca vitis gothe trapping agent, the trapping agent is loaded to a rubber head to form a trapping core which is attached to a jasmine yellow glue board, trapping is conducted during the leafhopper peak period, and the tapping agent has the good trapping effect on the empoasca vitis gothe. According to the method, the plant smell of a tea plantation ecosystem is used for trapping the empoasca vitis gothe, so that the environment is protected, and biological diversity is not damaged. The plant smell is used for trapping, preventing and controlling the empoasca vitis gothe, and using frequency and dosage of chemical pesticide are reduced. The method is simple and convenient to implement, and low in cost. The prepared empoasca vitis gothe trapping agent is high in trapping efficiency, high in trapping speed, stable in performance, and capable of effectively trapping the empoasca vitis gothe, and the preventing and controlling effect reaches 60%.
Description
Technical field
The invention belongs to Pests of Tea-Plants and prevent and treat method, relate to the information substance containing in weed in tea garden rosemary, Xue MingRosma rinus officinalis smell, these information substances are screened, form false eye leafhopper attractant, add synergist, the sustained release agent of suitable dosage, form the efficient attractant of false eye leafhopper; Invested the yellow haftplatte of jasmine, in this leafhopper insect population peak period, use, can be obtained the good control efficiency to this leafhopper when adult percentage surpasses some in tea place.
Background technology
False eye leafhopper
be Chinese tea place the most serious insect so far, with adult and nymph thorn, inhale the tender tip of tea tree of causing harm; Female worm lays eggs in tender stem, destroys tender stem conducting tissue, affects the normal conveying of tea tree nutrient and moisture, causes bud-leaf growth retardation.This insect is distributed widely in Chinese tea district, from Qingdao, Shandong Province Cha Qu to Sanya, Hainan Province Cha Qu, there are every year for 7～22 generations, on mid-May～late July, 2 insect population peaks of early September～late October shape, in its peak period, as not dispenser, 3～5 days cause harm just makes tea place shape as burnt.Present stage mainly adopts chemical control, and the annual dispenser in many tea district more than 10 time causes this leafhopper and produces stronger pesticide resistance, and dispenser causes that tealeaves agriculture is residual to exceed standard often.For effectively controlling this leafhopper, we have inquired into the regulation and control of weed in tea garden rosemary, Xue MingRosma rinus officinalis smell to this leafhopper behavior.
This leafhopper is former is a kind of polyphagy insect on weeds and undershrub, in 50～sixties of 20th century, and China's large area kind of tea of reclaiming wasteland, it is transferred to tea tree and becomes sociales from weeds and undershrub.In present stage China mountain forest, in seminatural tea place, tea tree is with weeds, undershrub together with arbor intermingle, and this leafhopper is slight to causing harm of tea tree, may be that it is transferred on some weeds and has endangered.Tea place common weed rosemary, Xue MingRosma rinus officinalis is selected in this research, detect its smell this leafhopper had to seduction? select wherein active component and make leafhopper attractant, add sustained release agent, synergist to lure effect with strengthening, being loaded in rubber tip makes and lures core again, invest and lure on worm colour table, the allure of stack colour table lures effect further to promote.In leafhopper insect population peak period, tea place, use trapping bait colour table trapping leafhopper, inquire into the approach with weed in tea garden smell regulation and control leafhopper.
Extensively probed in the world in the last few years the information substance that contains in plant scent or volatile matter for the effect of preventing and treating of insect." push-pull " theory and application technology thereof have more been studied in the world, one of principle is exactly to utilize plant volatile regulation and control insect and natural enemy behavior thereof, in main cultivation crop region, cause to be unfavorable for the condition of insect existence and insect to be pushed away to (push) main cultivation crop, make it trend towards trap crop (pull).The plant protection expert of Australia selects the active component that comes from multiple host and non-host plant to form " super-blending " (super mixture), effectively traps the female moth of cotton bollworm Helicoverpa armigera (H ü bner).Sri Lanka's intercropping in some tea place lures worm plant, to lure important pests angle, tea place fleahopper etc.; In south India tea place, place the branch of the composite family ornamental plants Montanoa bipinnatifida C.Koch in rainforest plant, trap important Pests of Tea-Plants tea twig beetle worm Xyleborus fornicatus Eichhoff etc. strongerly, all can partly alleviate insect pest.Therefore the pheromones, containing in plant scent can be used as one of means of insect prevention and control.
Summary of the invention
The object of the invention is to provide a kind of method of utilizing weed in tea garden rosemary, Xue MingRosma rinus officinalis smell trapping false eye leafhopper, by following steps, realizes:
(1) take false eye leafhopper as examination worm, take rosemary, Xue MingRosma rinus officinalis plant as taste source, in indoor, carry out behavior determination, thereby affirmed rosemary, Xue MingRosma rinus officinalis smell lure false eye leafhopper.
The weed in tea garden rosemary, Xue MingRosma rinus officinalis plant smell of take is taste source, and the false eye leafhopper of take carries out behavior determination as trying worm, and when taste source dosage increases, the leafhopper number of luring also increases, and when taste source dosage surpasses certain threshold value, the leafhopper number of luring reduces; The leafhopper number (y) of being lured (x) becomes parabolic relation y=-0.0943x with supplying tasting source quality
2+ 1.1141x+9.5169, R
2=0.9666(P<0.05).Therefore think that rosemary, Xue MingRosma rinus officinalis smell lures this leafhopper significantly.
(2) use air to carry absorption method secretly, from rosemary, Xue MingRosma rinus officinalis plant smell, separation identifies 46 kinds of components, by 25 kinds of components that wherein content is larger, as taste source, carry out behavior determination, find that Camphor, Caryphyllene, (R)-(-)-α-Phellandrene, α-Terpineol and Eucalyptol have remarkable attractive activity to this leafhopper.
(3) take n-hexane as solvent, Camphor, Carphyllene and α-Terpineol are made into respectively to 10
-2g/ml solution, then three is mixed with to pheromones mixture in 1:1:1 ratio, in tea place, detect, find that this mixture repels this leafhopper.Take n-hexane as solvent, and it is 10 that Camphor, Caryphyllene, (R)-(-)-α-Phellandrene, α-Terpineol and Eucalyptol are made into respectively to concentration
-6g/ml-10
-1the odor source of g/ml, with 1:0.1-15:0.1-15:0.1-15:0.1-15 ratio formulated in combination, become false eye leafhopper trapping bait again, the false eye leafhopper trapping bait of preparation is loaded in rubber tip, make and lure core, lure core to invest on the yellow haftplatte of jasmine (with reference to patent of invention " a kind of method of trapping, preventing and controlling false eye leafhopper ", patent No. ZL201110134803.2, embodiment 1, but the colour table color that the present invention uses is that jasmine Huang rather than bud are green, colour table shape is square rather than rectangle, length of side 25cm), in this leafhopper insect population peak period, when adult percentage>=40%, implement trapping, find that it lures this leafhopper strongly, very strong attractant effect is described.
(4) or with trapping bait following methods replacement step (3) Suo Shu: add again γ-Terpinene to form six component mixtures in five kinds of components of step (2), six kinds of components are all made into respectively to 10
-6g/ml-10
-1the odor source of g/ml, with the assembly of 1:0.1-15:0.1-15:0.1-15:0.1-15:0.1-15 ratio, has formed the efficient trapping bait of false eye leafhopper.Field trapping evidence six component mixtures lure than five component mixtures that effect is better, trapping speed is faster.When this leafhopper insect population is contained the phase of sending out, adult percentage >=40%, the efficient trapping bait of six component leafhopper is invested to the yellow haftplatte of jasmine, can effectively trap false eye leafhopper, preventive effect breaks through 60%.
(5) draw the recess of 1 rubber tip of the efficient trapping bait injection of false eye leafhopper of 250 μ 1 at every turn, a few minutes add the atoleine of 10 μ 1 again as sustained release agent, by the time trapping bait, sustained release agent all immerse in rubber tip, just make one and lure core, store under low temperature standby.Also can will lure the effect Increased Plasma Half-life a few days, can increase trapping leafhopper quantity, trap leafier cicada.
Another object of the present invention is to provide the application of described method in trapping false eye leafhopper.
Trapping bait prepared by the inventive method also can be used for the green prevention and control of false eye leafhopper.
The present invention utilizes the most important insect in information substance in the common weed rosemary, Xue MingRosma rinus officinalis smell of tea place trapping tea place---false eye leafhopper, can reduce or remit the usage amount of chemical pesticide and reduces pollution.Be to utilize the holocoenotic plant scent trapping in tea place false eye leafhopper, be beneficial to protection of the environment, do not injure bio-diversity; Use the little greenery cicada of plant scent trapping, preventing and controlling artificial eye, access times and the dosage of deduction and exemption chemical pesticide; The inventive method is simple and easy to do, and cost is low, and prepared leafhopper trapping bait lures that effect is high, trapping speed is fast, stable performance, can effectively trap false eye leafhopper, and preventive effect breaks through 60%.
Accompanying drawing explanation
Fig. 1 selects rosemary, Xue MingRosma rinus officinalis taste source or CK(air) false eye leafhopper number.
Fig. 2 is the TIC of each component in rosemary, Xue MingRosma rinus officinalis smell.
Fig. 3 is the false eye leafhopper quantity that rosemary, Xue MingRosma rinus officinalis five components lure core trapping.
Fig. 4 is the false eye leafhopper quantity that rosemary, Xue MingRosma rinus officinalis five component slowly-releasings lure core trapping.
Fig. 5 is the false eye leafhopper quantity that Blend1 lures core trapping.
Fig. 6 is the false eye leafhopper quantity that Blend1 slowly-releasing lures core trapping.
Fig. 7 be the time period on 7 of July 22 tea place leafhopper fly to 4 kinds and lure the quantity of core to be significant temporal rhythm, the difference indicating in figure between the pillar of same color of different lowercases reaches significance level.
Fig. 8 be the time period on 7 of July 23 tea place leafhopper fly to 4 kinds and lure the quantity of core to be significant temporal rhythm, the difference indicating in figure between the pillar of same color of different lowercases reaches significance level.
Fig. 9 be the time period on 7 of July 24 tea place leafhopper fly to 4 kinds and lure the quantity of core to be significant temporal rhythm, the difference indicating in figure between the pillar of same color of different lowercases reaches significance level.
Figure 10 be the time period on 7 of July 25 tea place leafhopper fly to 4 kinds and lure the quantity of core to be significant temporal rhythm, the difference indicating in figure between the pillar of same color of different lowercases reaches significance level.
Figure 11 be the time period on 7 of July 26 tea place leafhopper fly to 4 kinds and lure the quantity of core to be significant temporal rhythm, the difference indicating in figure between the pillar of same color of different lowercases reaches significance level.
Embodiment
The present invention is further described in conjunction with the accompanying drawings and embodiments.
Embodiment 1
The 1st step rosemary, Xue MingRosma rinus officinalis smell is lured the test of false eye leafhopper
1. taste source
Rosemary, Xue MingRosma rinus officinalis Rosmarinus officinalis L. is the weeds of self-sow in China Measures Institute tea place, and during test, the tender part of clip children is lured false eye leafhopper as taste source material to discharge smell.If 1g, 2g, 3g, 5g, 7g, 9g be totally 6 dosage, each dosage retest 5 times.
2. for examination insect
From China Measures Institute tea place, collect female, male false eye leafhopper adult, be placed on the potted plant tea tree in dependent insect cage and raise, allow its free mating, lay eggs.Treat ovum hatching, grow into 3 ages during nymph as examination worm.Test before half an hour get 3 age nymph standby in test tube, fasting.
3.Y shape olfactometer and biologicall test program
Y tube is made by colourless transparent glass, and two arms and base portion are 10cm, and internal diameter is 1.0cm, and two arm angles are 90 °.Two arms connect respectively taste source bottle (or control bottle), humidification bottle, air filtering bottle (inside filling the active carbon having activated) and flowmeter, between each parts, all with Teflon pipe, connect.
During mensuration, regulating two arm flows is 100ml/min, and the 10min that first bleeds before each mensuration, makes to be full of smell in the arm of Y tube taste source.During test with dactylethrae by 13 age false eye leafhopper from Y tube base portion, introduce, when false eye leafhopper enters Y tube one arm the 5cm that creeps against the wind, counting.Every kind of dosage retest 5 times is tested 20 at every turn, and every only with once; 10 of every tests, clean Y pipe inside and outside wall with 75% ethanol, dry the link position of transposing Y tube two arms and taste source bottle and control bottle, the impact causing to eliminate possible asymmetric of Y tube.After each dosage tests, Y tube, taste source bottle, control bottle, humidification bottle are cleaned with potassium bichromate, 100 ℃ of oven for drying, active carbon is placed in activation 4h desorption in 100 ℃ of baking ovens, eliminates the gas of absorption, standby.
Biologicall test is carried out in darkroom, and above Y pipe, 1.5 meters have 1 5W incandescent lamp that illumination is provided.Testing time 8:00 to 16:00, during this, this leafhopper is more active.26 ℃ of room temperatures, relative moisture 70%.
4. data analysing method
Analyze the dependency relation between rosemary, Xue MingRosma rinus officinalis dosage and the leafhopper quantity of its attraction.
5. result: rosemary, Xue MingRosma rinus officinalis plant smell is lured false eye leafhopper significantly
The smell of each dosage rosemary, Xue MingRosma rinus officinalis plant all presents the effect of luring leafhopper, and along with the increase of rosemary, Xue MingRosma rinus officinalis dosage, lures the leafhopper quantity of coming also to increase; And when dosage surpasses certain threshold value, lure the leafhopper quantity of coming to present and reduce trend.When rosemary, Xue MingRosma rinus officinalis dosage is (x) 1g, 2g, 3g, 5g, 7g, 9g, the leafhopper mean (y) in trend taste source is correspondingly 10.5,11.25,12.25,12.75,12.50,12.00, and the two is parabolic relation y=-0.0943x
2+ 1.1141x+9.5169, R
2=0.9666(P<0.05) (referring to Fig. 1).
In the 2nd step rosemary, Xue MingRosma rinus officinalis smell, the separation of volatile component is identified
1. the extraction of rosemary, Xue MingRosma rinus officinalis plant volatiles
The tender stem of clip 25g rosemary, Xue MingRosma rinus officinalis is put into the cylinder of glass quality, and this cylinder is comprised of two parts, and closely break-in also can be opened, and puts into new fresh and tender stem and tightens afterwards.Respectively there are an air inlet and gas outlet in cylinder two ends, air inlet connects an air cleaner (the in-built active carbon having activated) and flowmeter successively, gas outlet connects Super Q adsorption column (100mg), water absorber, air pump successively, and each several part connects with Teflon pipe.Bleed after 12h, take off Super Q adsorption column, with 600 μ l chromatographically pure n-hexane drip washing, in the solution access brown vial under drip washing, and add 10 μ l10
– 4g/ml ethyl caprate, as interior mark, shakes up rear use microsyringe and draws 1 μ l injection gas chromatograph-mass spectrometer.
2.GC-MS measures
The GC-MS instrument model that this experiment adopts is Agilent GC (6890N)-MS (5975B).Chromatographic column is 30.0m * 250um * 0.25um id HP-5MS quartz capillary column.Splitless injecting samples; Constant flow rate, flow is 1.0ml/min.Solvent delay 3min.250 ℃ of injector temperatures, 280 ℃ of GC-MS interface temperature.Temperature programming: 50～190 ℃ of column temperatures, initial 50 ℃ and keep 5min, with 3.5 ℃ of min
-1speed rises to 141 ° of C, then with 2 ℃ of min
-1rise to 171 ℃, finally with 3.5 ℃ of min
-1speed rises to 190 ℃, at 190 ℃, keeps 5min.EI ion gun, ionization energy 70eV, is used full scan, and scan frequency is 2 times/s.Carrier gas is 99.999% helium.
Qualitative method: (1) injects n-compound according to same test program to GC-MS, and the parameters such as the retention time according to n-compound on GC-MS are determined the component in rosemary, Xue MingRosma rinus officinalis smell; (2) according to the matching degree of the mass spectrogram of component in rosemary, Xue MingRosma rinus officinalis smell and GC-MS chem workstation Plays thing collection of illustrative plates.Quantitative approach: per sample, the peak area of each component carries out relative quantification for the ratio of interior mark peak area.
3. result: various components and content thereof in rosemary, Xue MingRosma rinus officinalis smell
From rosemary, Xue MingRosma rinus officinalis smell, detect altogether 46 kinds of volatile compounds (Fig. 2, table 1), comprise a kind of 9 kinds of alcohols, 4 kinds of ketones, 4 kinds of ester classes, a kind of acids, 18 kinds of olefines, 7 kinds of alkanes, a kind of ethers, a kind of aldehydes and phenols.In this 9 compounds, the content of alcohols is maximum, accounting for 52.20% of total content, is secondly respectively alkene (34.51%), ketone (10.06%), ester (1.84%), alkane (0.57%), ether (0.48%), aldehyde (0.22%) and phenol (0.07%), and what content was minimum is acid (0.04%).In these 46 kinds of volatile components, the content of cineole is maximum, accounts for 42.54% of total content, is secondly australene (10.37%), is verbenone (6.82%) again.
Each component detecting in table 1 rosemary, Xue MingRosma rinus officinalis smell and with respect to interior target amount
The indoor Y pipe of the 3rd step olfactometer detects the activity that in rosemary, Xue MingRosma rinus officinalis smell, main component is lured leafhopper
1, from the rosemary, Xue MingRosma rinus officinalis smell of identifying, select 25 kinds of components (table 2) that content is larger: 1. the content of every kind of composition accounts for total amount >=0.73% of 46 kinds of compounds that detect, i.e. cineole, α firpene, verbenone, 3-carene, laurene, borneol, caryophyllene, terpinolene, thatch ketone, beta pinene, linalool, Bronyl acetate, 4-carene, α terpineol, α phellandrene, amphene, terpineol; 2. the compound of luring other kind insect of having reported in pertinent literature, as geraniol, eugenol methyl ether, geranial, neryl acetate, citronellol, ocimene, verbenol, esdragol.
Take n-hexane as solvent, respectively 25 kinds of one-tenth are distributed into 10
– 6g/ml, 10
– 4g/ml and 10
– 2g/ml Three doses, draws 1ml as taste source, draws n-hexane 1ml as CK, carries out behavior determination; Find after measured active stronger component, then be made into 10
– 8g/ml and 10
– 10two kinds of dosage of g/ml continue to measure.Examination worm and mensuration program are with the 1st step " 3Y shape olfactometer and biologicall test program ".
2, result
χ
2test result shows: 10
-2g/ml Camphor, 10
-4g/ml Caryphyllene and 10
-6g/ml and 10
-8g/ml (R)-(-)-α-Phellandrene, and 10
-4g/ml and 10
-10g/ml α-Terpineol, 10
-10g/ml and 10
-8totally 8 dosage of g/ml Eucalyptol has remarkable seduction (table 2) to leafhopper.
Table 2 is significantly lured main component and the dosage thereof in the rosemary, Xue MingRosma rinus officinalis smell of false eye leafhopper
The high dose mixture seduction of three components in the 4th step rosemary, Xue MingRosma rinus officinalis smell
1, with CK(air) compare, Camphor, α-Terpineol and Carphyllene respectively have 1 or 2 dosage significantly to lure false eye leafhopper (P<0.05) (table 2), and the n-hexane of take is respectively made into 10 by these 3 kinds of compounds as solvent
-2g/ml solution, then be made into mixture in 1:1:1 ratio.Each 250 μ l mixture solutions of drawing are loaded in 1 rubber tip, make 1 and lure core, are called rosemary, Xue MingRosma rinus officinalis three components and lure core.Respectively cis-blatter alcohol, trans-1-hexenoic aldehyde and linalool are made and lured core again, be called Blend1 and lure core, this is a kind of leafhopper trapping bait of this assembly system, see (2012) .Behavioral responses for evaluating the attractiveness of specific tea shoot volatiles to the tea green leafhopper such as Mu, Empoaca vitis.Insect Science, 2012,19 (2): 229～238.Each 250 μ l10 that draw
-2g/ml LINL-OX is loaded in 1 rubber tip, makes 1 and lures core, is called LINL-OX and lures core.Each 250 μ l n-hexanes of drawing are loaded in and in 1 rubber tip, make 1 and lure core, are called n-hexane and lure core.Rear 3 all as CK.
In false eye leafhopper insect population peak period, by every kind, lure core to invest on the yellow haftplatte of jasmine respectively, 1 attached 1 of haftplatte lures core, haftplatte to be hung on small wooden, waddy insert in tea capable in, the base of haftplatte is in abutting connection with the tea tip, between haftplatte, distance is 7～8 meters.Every kind lures core to repeat 30 times.Investigate the leafhopper quantity of traping on every block of plate after 24 hours, on June 18th, 2013 link plate, continuous observation on the 19th～21 3 days, each investigation completes at 13:00～14:00.After each investigation, adjust the orientation of colour table to trap equably the leafhopper from all directions.(lure core and haftplatte with reference to patent of invention " a kind of method of trapping, preventing and controlling false eye leafhopper ", patent No. ZL201110134803.2, embodiment 1, but the colour table color that the present invention uses is that jasmine Huang rather than bud are green, colour table shape is square rather than rectangle, length of side 25cm)
4 kinds of leafhopper quantity that lure core to trap are done to variance analysis, then use the new multipole difference analysis of Duncan ' s method comparing difference.
2, result
Lure core to compare with n-hexane, Blend1 lures core to have remarkable seduction, and LINL-OX lures core to have repelling effect, and rosemary, Xue MingRosma rinus officinalis three components lure core to have remarkable repelling effect (table 3).
False eye leafhopper quantity and difference thereof that table 3 rosemary, Xue MingRosma rinus officinalis three components lure core etc. to lure for three days on end
Note: the difference indicating in same row between the numerical value of different lowercases reaches significance level (P<0.05), indicates difference between the numerical value of identical lowercase not significantly (P>0.05).Repeat 30 times.
Five component mixtures and add the mixture of sustained release agent to lure leafhopper test in the 5th step rosemary, Xue MingRosma rinus officinalis smell
1 lures core in table 2, significantly to lure 10 of leafhopper
-2g/ml Camphor, 10
-4g/ml Caryphyllene, 10
-6g/ml (R)-(-)-α-Phellandrene, 10
-4g/ml α-Terpineol and 10
-8g/ml Eucalyptol is in 1:1:1:1:1 ratio wiring solution-forming, and every absorption 250 μ l are loaded in and in 1 rubber tip, make 1 and lure core, are called rosemary, Xue MingRosma rinus officinalis five components and lure core.According to five components, lure again the production process of core, finally in luring core, add 10 μ l atoleines as sustained release agent, to slow down the rate of release of information substance, be called rosemary, Xue MingRosma rinus officinalis five component slowly-releasings and lure core.
The Blend1 of take lures core as CK.According to Blend1, lure core method for making, finally in often luring core, add 10 μ l atoleines as sustained release agent, be called Blend1 slowly-releasing and lure core.
2 test and analytical methods
According to the trapping program of the 4th step " 1 ", in a leafhopper Sheng phase, 4 kinds are lured core invest respectively on the yellow haftplatte of jasmine, be positioned in tea place the leafhopper quantity of traping on investigation colour table for continuous 20 days.Within every 3 days, change the yellow plate of jasmine 1 time, change altogether 3 times.Every kind lures core to repeat 30 times.
Do following analysis: (1) does variance analysis, comparative effectiveness for the difference between these the 4 kinds leafhopper quantity that lure core trapping.(2) to analyze every kind of leafhopper quantity (y) that lures trapping core every day and the number of days dependency relation between (x), calculate regression model, obtain the half life period, does does analysis sustained release agent extend and lure effect?
3 results
In 3.1 rosemary, Xue MingRosma rinus officinalis smells, five component moderate dose mixtures are significantly lured false eye leafhopper
As shown in Fig. 3, Fig. 4, Fig. 5 and Fig. 6, July 21 to August 9 was investigated 15 times altogether, traped leafhopper quantity as follows on average every block of plate: it is that to lure core be that to lure core be that to lure core be 46.0 for 37.2, Blend1 slowly-releasing for 38.6, Blend1 for 42.5, five component slowly-releasings that five components lure core.Variance analysis shows that 4 kinds of differences between the leafhopper quantity that lures core trapping are not remarkable.
3.2 sustained release agents lure core to lure the prolongation effect of effect for rosemary, Xue MingRosma rinus officinalis volatile matter five components and Blend1
As shown in Fig. 3, Fig. 4, Fig. 5 and Fig. 6: (1) five component slowly-releasing lures the effect of core to start to surmount five components at the 10th day and lures core; (2) Blend1 slowly-releasing lures core to start to surmount Blend1 at the 3rd day and lures core.By Fig. 3, Fig. 4 calculate five components to lure the half life period of core be that to lure the half life period of core be 13.22 days 11.82 days, five component slowly-releasings, postpone 1.4 days; By Fig. 5, Fig. 6 calculate Blend1 to lure the half life period of core be that to lure the half life period of core be 13.93 days 10.75 days, Blend1 slowly-releasing, postpone 3.2 days.
Five components and the effect that adds the mixture of synergist to lure leafhopper in the 6th step rosemary, Xue MingRosma rinus officinalis smell
1,, according to test procedure in the 5th step tea place, select the information substance thing of the 5th step: 10
-2g/ml Camphor, 10
-4g/mlCaryphyllene, 10
-6g/ml (R)-(-)-α-Phellandrene, 10
-4g/ml α-Terpineol and 10
-8g/ml Eucalyptol, and from peppermint, touch the γ-Terpinene of perfume (or spice) and separated evaluation the careless volatile matter of taking drugs, be mixed with 10
-10g/ml.In 1:1:1:1:1:1 ratio, draw these 6 kinds of solution and make mixture, be above loaded in the core that lures that rubber tip makes and lure core six components.
Select Blend2,10
-2g/ml (instead)-2-hexenoic aldehyde, 10
-2g/ml (suitable)-blatter alcohol, 10
-2g/ml2-amylene-1-ol, 10
-2g/ml (instead)-2-pentenals, 10
-2g/ml (suitable)-3-hexene acetic acid esters, 10
-2g/ml amylalcohol, 10
-2g/ml hexanol and 10
-2the equal amount of mixture of g/ml1-amylene-3-alcohol.Select Blend1,10
-2the cis-blatter alcohol of g/ml, 10
-2the trans-1-hexenoic aldehyde of g/ml and 10
-2the equal amount of mixture of g/ml linalool.Make two kinds and lure core, the n-hexane of take lures core as CK.July 9,8:00～8:30 lured the colour table of core to be put in tea place by having, the leafhopper quantity of traping every 24 hours respectively at 8:00～8:30 investigation on July 10～13.Every kind lures core to repeat 30 times.For lure 4 kinds of every days core leafhopper quantity variance do variance analysis, and with Duncan ' s multiple range test comparing difference.
2, result lures core with CK(n-hexane) to compare, six components lure core, Blend2 to lure core and Blend1 to lure core to have remarkable attractant effect (P<0.05); Six components lure core, Blend2 to lure core to be better than Blend1; A few days ago, six components lure the effect of core better than Blend2 in test; The effect of latter two days Blend2 of test surmounts six components and lures core (table 4).Blend2 is a kind of trapping bait of the false eye leafhopper of this group screening, see (2012) .Behavioral responses for evaluating the attractiveness of specific tea shoot volatiles to the tea green leafhopper such as Mu, Empoaca vitis.Insect Science, 2012,19 (2): 229～238
The comparison of table 43 kind of leafhopper timber quarantine false eye leafhopper effect
Note: the difference indicating in same row between the numerical value of different lowercases reaches significance level (P<0.05), indicates difference between the numerical value of identical lowercase not significantly (P>0.05).Repeat 30 times.
In embodiment 2 tea places, leafhopper is flown to the temporal rhythm that five components lure core
1, adopt 4 kinds in embodiment 1 the 5th step to lure core, invest respectively on the yellow haftplatte of jasmine, with reference to the 5th step program, in tea place, test, every kind lures core to repeat 30 times.Following 7 time period: 18:00-6:00,6:00-8:00,8:00-10:00,10:00-12:00,12:00-14:00,14:00-16:00 and 16:00-18:00 will be divided into for one day.Leafhopper number on investigation of last 30 minutes of each time colour table.From 21～July 26 July, carried out the temporal rhythm investigation of 6 days by a definite date.
For the difference between every kind of leafhopper quantity that lures 7 time period trappings core every day, do variance analysis, and with the poor comparing difference of the new multipole of Duncan ' s, prove that in rosemary, Xue MingRosma rinus officinalis smell, five components lure the attractant effect of core remarkable.
2, result
Every kind lure core be all with 18:00～6:00 time period lure leafhopper maximum, with At All Other Times section difference reach significance level (P<0.05); In trapping in continuous 5 days, five components lure core, five component slowly-releasings lure core, Blend1 lure core and Blend1 slowly-releasing lure core leafhopper number account for respectively lure separately to obtain 75%, 71%, 74% and 75%(Fig. 7, Fig. 8, Fig. 9, Figure 10, Figure 11 of leafhopper sum).Because this leafhopper statvolt at night, on tea tree, therefore dusk and dawn are the main activities periods of this leafhopper, should be noted in this time period trapping.
Embodiment 3 rosemary, Xue MingRosma rinus officinalis smell six components lure core in field the preventive effect for false eye leafhopper
1, at the Cha Qu of Anhui Province, early September～late November is false eye leafhopper insect population peak period, selects Nanma Gu Shanchachang production tea place, Anhui on September 8.Arrange: 20 six components lure the yellow haftplatte/667m of core+jasmine
2; Process 2:20 Blend2 and lure the yellow haftplatte/667m of core+jasmine
2; Process 3:20 n-hexane and lure the yellow haftplatte/667m of core+jasmine
2; Process 4:0 trapper/667m
2be CK.Every kind lures core to repeat 3 times, and the area of each repetition is 667m
2tea place.
Morning September 8,10:00～12:00, processed 5 samplings in 12Ge communities respectively at 4 kinds, and it is capable that every sampling point is got 1 meter of tea, is longitudinally divided into two, and firmly pats tea branch, with Polypropylence Sheet, accepts, and counting shakes, and fallen leafhopper becomes, nymph.14:00～16:00 will lure core to be suspended to colour table central authorities, and colour table is suspended from small bamboo and forms trapper, and trapper is evenly positioned in tea place, spacing 7m * 8m.Little bamboo bar inserts in the middle of tea clump, and colour table base and the tea tip are contour.
September 10, the same five point sampling investigation leafhopper quantity of using, investigated the leafhopper quantity of catching on the yellow haftplatte of jasmine.Duration of test became a fine day without rain day, 30～35 ℃ of temperature, relative moisture 70%～80%.
2, result
Blend2 be this group screening for false eye leafhopper, have the leafhopper trapping bait of traping more by force effect, once the preventive effect on first insect population peak of this leafhopper can reach 59.3%.This duration of test tea place adult is more, and the preventive effect of Blend2 surpasses 60%, and still, six components lure the better effects if (table 5) of core.
In table 5 tea place, 4 kinds lure core for the comparison of false eye leafhopper preventive effect
Note: proofread and correct insect population decline rate=(trapping area insect population decline rate-check plot insect population decline rate)/(1-check plot insect population decline rate) * 100%.The difference indicating between the numerical value of different lowercases reaches significance level.
Claims (5)
1. a method of utilizing weed in tea garden rosemary, Xue MingRosma rinus officinalis smell trapping false eye leafhopper, is characterized in that, by following steps, realizes:
(1) take false eye leafhopper as examination worm, take rosemary, Xue MingRosma rinus officinalis plant as taste source, in the indoor behavior determination that carries out, determine rosemary, Xue MingRosma rinus officinalis smell lure false eye leafhopper;
(2) use air to carry absorption method secretly, from rosemary, Xue MingRosma rinus officinalis plant smell, separation identifies 46 kinds of components, determines that Camphor, Caryphyllene, (R)-(-)-α-Phellandrene, α-Terpineol and Eucalyptol have remarkable attractive activity to this leafhopper;
(3) take n-hexane as solvent, it is 10 that these five kinds of active components are made into respectively to concentration
-6g/ml-10
-1the odor source of g/ml, again with 1: 0.1-15: 0.1-15: 0.1-15:0.1-15 ratio formulated in combination becomes false eye leafhopper trapping bait, this trapping bait is loaded in rubber tip and makes and lure core, invest on the yellow haftplatte of jasmine, in this leafhopper insect population peak period, implement trapping;
(4) in the efficient trapping bait of false eye leafhopper, add 10
atoleine as sustained release agent, extend and to lure the effect half life period, increase trapping leafhopper quantity.
2. a kind of method of utilizing weed in tea garden rosemary, Xue MingRosma rinus officinalis smell trapping false eye leafhopper according to claim 1, it is characterized in that, the described trapping bait of step (3) replaces with: in five kinds of components of step (2), add γ-Terpinene to form six component mixtures, being made into concentration is 10 again
-6g/ml-10
-1the odor source of g/ml, with 1: 0.1-15: 0.1-15: the ratio formulated in combination of 0.1-15:0.1-15:0.1-15 becomes the efficient trapping bait of false eye leafhopper.
3. a kind of method of utilizing weed in tea garden rosemary, Xue MingRosma rinus officinalis smell trapping false eye leafhopper according to claim 1, it is characterized in that: it is taste source that step (1) be take weed in tea garden rosemary, Xue MingRosma rinus officinalis plant smell, the false eye leafhopper of take carries out behavior determination as trying worm, when taste source dosage increases, the leafhopper number of luring also increases, when taste source dosage surpasses certain threshold value, the leafhopper number of luring reduces; The leafhopper number (y) of being lured (x) becomes parabolic relation y=-0.0943 x with supplying tasting source quality
2+ 1.1141x+9.5169, R
2=0.9666(P
0.05).
4. the application of a kind of method of utilizing weed in tea garden rosemary, Xue MingRosma rinus officinalis smell trapping false eye leafhopper according to claim 1 and 2 in trapping false eye leafhopper.
5. a kind of method of weed in tea garden rosemary, Xue MingRosma rinus officinalis smell trapping false eye leafhopper of utilizing according to claim 1 and 2 is applied in the green prevention and control of false eye leafhopper.
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