CN106857508A - The trans dilute Zuo Wei of China pink shoulder green plant bug attractant application - Google Patents
The trans dilute Zuo Wei of China pink shoulder green plant bug attractant application Download PDFInfo
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- CN106857508A CN106857508A CN201611229325.2A CN201611229325A CN106857508A CN 106857508 A CN106857508 A CN 106857508A CN 201611229325 A CN201611229325 A CN 201611229325A CN 106857508 A CN106857508 A CN 106857508A
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- dilute
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- green plant
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- china pink
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
Abstract
The present invention relates to the dilute Zuo Wei of trans China pink shoulder green plant bug attractant application, the dilute absolute ethyl alcohol of trans China pink is made into mother liquor, is used for field after paraffin oil or n-hexane dissolution, the 10000ppm Zuo Wei of concentration 10 shoulder green plant bug attractant.The behavior reaction of both sexes adult is determined by Y-shaped smell sensing apparatus, when determining concentration for 50ppm, rate up to 36.67% is lured to male worm;If H types olfactometer determines the behavior reaction of stinkbug, when determining concentration for 50ppm, rate up to 48.65% is lured.Solarium tests, and when determining concentration for 500ppm, lures rate up to 65.84%.Field test, when determining concentration 10000ppm, if pair stinkbug lures rate up to 37.16%, to luring rate up to 20.74% into stinkbug.Present invention determine that anti-China pink it is dilute can be used in field can Yin You shoulder green plant bug, strengthen regulation and control to natural enemy, control rice grub insect density.Also the behavior of each worm state can indoors be adjusted.
Description
Technical field
The present invention relates to the dilute Zuo Wei of trans-China pink shoulder green plant bug attractant application.
Background technology
Mirid, cyrtorhinus are the important natural enemy insects of rice field, are mainly made a living with plant hopper and leafhopper, plant hopper are most liked, in field
Between take food brown paddy plant hopper ovum ratio up to 30%-70% (Zhou, 1986), can take food within one day 7.5-10.2 brown paddy plant hopper (Reyes,
1975).There is research report, aggreation is shown in plant hopper density cell Mirid, cyrtorhinus higher, but it is to brown paddy plant hopper
Ingestion rate is unrelated with plant hopper density, and in stinkbug:Lice quantity ratio reaches 1:1,1:8,20:When 1, stinkbug can be in no chemical pesticide
Under the conditions of, control plant hopper insect density (Tanangsnakool, 1975).Mirid, cyrtorhinus are sensitive to chemical pesticide, pymetrozine etc.
Chemical pesticide influences its ability and to the control dynamics of rice grub (Zhang et al., 2015) of looking for food.And do not have temporarily
Find the chemical pesticide and insecticide-applying way to Mirid, cyrtorhinus safety.Therefore researcher is directed to Mirid, cyrtorhinus attractant exhibition
Research is opened, the extraction of Rice Volatiles has been substantially carried out and has been looked for the compound monomer (Cheng, 2001) for having attracting action,
But find can apply to field lure method.
The content of the invention
The purpose of the present invention is directed to above-mentioned present situation, it is desirable to provide one kind can be used in field, strengthens the tune to natural enemy
Control act on so that control rice grub insect density the dilute Zuo Wei of trans-China pink shoulder green plant bug attractant exploitation with application.
The implementation of the object of the invention is that trans-China pink is dilute is dissolved to 10000ppm in absolute ethyl alcohol, uses paraffin oil
Or n-hexane is when being diluted to concentration 10ppm -10000ppm, Zuo Wei shoulder green plant bug attractant.
The present invention determines the dilute behavior reaction to Mirid, cyrtorhinus adult of trans-China pink by Y-shaped smell sensing apparatus, it is determined that instead
Formula-China pink dilute , Dui when concentration is 500ppm shoulder green plant bug male worm lure rate up to 36.67%;H types olfactometer determines black porch
The green plant bug behavior reaction dilute to trans-China pink, if determine the dilute , Dui in concentration 50ppm of trans-China pink shoulder green plant bug stinkbug institute
Rate up to 48.65% must be lured.Solarium tests, when determining that concentration is 500ppm , Dui shoulder green plant bug lure rate up to 64.84%.
Field test, if it is determined that when concentration is 10000ppm , Dui shoulder green plant bug stinkbug lure rate up to 37.16% , Dui shoulder it is green blind
Stinkbug lures rate up to 20.74% into stinkbug.
By present invention may determine that trans-China pink it is dilute can lure be used in field Yin You shoulder green plant bug, so as to strengthen right
The regulating and controlling effect of natural enemy, controls rice grub insect density.
Brief description of the drawings
Fig. 1 is the H type olfactometer structural representations that the present invention is used,
Fig. 2 is Y-shaped smell sensing apparatus structural representation used by the present invention,
Fig. 3 is the Y-piece structural representation that the present invention is used;
Fig. 4 is solarium's test model figure,
Fig. 5 is solarium's experiment lure mode of suspension figure,
Fig. 6 is solarium's test operation figure;
Fig. 7 Shi Dui shoulder green plant bug attractant effect determine field experiment cell distribution map,
Fig. 8 is lure structural representation,
Fig. 9 is that lure specifically hangs and look into the worm location drawing;
If Figure 10 Shi Dui shoulder green plant bug stinkbug attractive activity figure,
Figure 11 Shi Dui the both sexes of shoulder green plant bug into stinkbug attractant effect test result figure,
Figure 12 be solarium Shi Yan Dui shoulder green plant bug attractant effect measurement result figure,
If Figure 13,14 be when field difference takes sample between shoulder green plant bug stinkbugs worm amount variation diagram,
Figure 15 be when field difference takes sample between shoulders green plant bug into stinkbug worm amount variation diagram,
Specific embodiment
Can be used to draw for the trans-China pink of confirmation is dilute and lure shoulder green plant bugs, the applicant has made following experiment.
If determining the Mirid, cyrtorhinus stinkbug behavior reaction dilute to trans-China pink using the H types olfactometer shown in Fig. 1.
The straight-arm 16cm long of H types pipe two, internal diameter 3cm, two straight-arms respectively have the opening that an internal diameter is 3cm, side away from the 4.5cm of bottom
Transverse arm at the opening of face with H type pipes is connected.Transverse arm 18cm long, internal diameter 3cm, have an internal diameter for 3cm in transverse arm middle, high
The cylinder open of 1cm, this is to connect insect population.It is connected with two straight-arms respectively at both ends open.
The dilute absolute ethyl alcohol of trans-China pink to be measured is configured to the mother liquor of 10000ppm, then with n-hexane dilute 8 times, 40
Again, 200 times, 1000 times, concentration is set to 1250ppm, 250ppm, 50ppm, 10ppm.H type pipes are lightly disposed across desktop
On, respectively with capillary draw 12 μ l trans-China pink is dilute and n-hexane (control treatment), be carefully put into H type olfactometers
In two straight-arms, then clogged at straight-arm top end opening with gauze.If again by 10 4 age Mirid, cyrtorhinus stinkbugs of hungry half an hour from
Connect and accessed in H type pipes at insect population, insect population is connect with careful the sealing of sealed membrane.Temperature is lain in for 28 ± 1 DEG C, humidity are
In 90% or so dark culturing case, and with the fully wrapped around firmly H type olfactometers of black cloth, if to exclude light to Mirid, cyrtorhinus stinkbug
The influence of housing choice behavior.If each H pipe accesses 10 4 age Mirid, cyrtorhinus stinkbugs of stalwartness, 60 are tested altogether.After observed and recorded 2h
If distribution situation of the Mirid, cyrtorhinus stinkbug in H type pipes.If if the Mirid, cyrtorhinus stinkbug of test is away from connecing insect population or so 1cm scopes
It is interior, it is designated as not making a choice.If the Mirid, cyrtorhinus of test are designated as if stinkbug is at A, B and at D having to odor source on the right side of H type pipes
Reaction;If conversely, being then designated as test Mirid, cyrtorhinus stinkbug selection H types pipe left side odor source.It is calculated as follows and lures rate.
Lure rate (%)=(treatment borer population-control borer population)/(treatment borer population+control borer population) × 100.
Data analysis:Using the significance of difference between the paired-samples T-test analyzing and processing group and control group of SPSS16.0.
Experimental result is as shown in Figure 10.As seen from the figure when concentration is relatively low, if trans-China pink Xi Dui shoulder green plant bug stinkbug
Have attracting action, and if when concentration is higher , Dui shoulder green plant bug stinkbug have repellent action, concentration be 50ppm when, lure rate most
It is high.
Can be obtained by interpretation of result, concentration be 10ppm when, if trans-China pink Xi Dui shoulder green plant bug stinkbug lure rate to reach
33.33% (P=0.07, t=2.449).Concentration be 50ppm when, if trans-China pink Xi Dui shoulder green plant bug stinkbug have extremely notable
Attracting action (P<0.01, t=8.104) rate up to 48.65%, is lured.When concentration reaches 250ppm, trans-China pink is dilute right
shoulder green plant bug lure rate up to 22.22% (P=0.315, t=1.117).And when concentration is 1250ppm, under indoor environment
If Dui shoulder green plant bug stinkbug have a significant repellent action, scattering ratio is up to 38.27% (P=0.004, t=4.448).Thus result
Analysis, trans-China pink it is dilute concentration be 50ppm when, if indoors environment Xia Dui shoulder green plant bug stinkbug have significant attractant effect,
Lure rate highest.So continue determine trans-China pink it is dilute concentration be 50ppm when , Dui shoulder green plant bug both sexes lured into stinkbug
Effect.
It is anti-the dilute behavior to Mirid, cyrtorhinus both sexes adult of trans-China pink to be determined using the Y-shaped smell sensing apparatus shown in Fig. 2,3
Should.
10000ppm is dissolved in absolute ethyl alcohol by trans-China pink is dilute, 50ppm is diluted to paraffin oil, as taste source,
Control group uses paraffin oil.
Take the Mirid, cyrtorhinus adult of just emergence, hungry 1h.Taste source bottle in be put into the 50ppm containing 40 μ L it is trans-stone
Bamboo is dilute or filter paper of neat solvent paraffin oil (CK), with the speed blowing air 20min of 250mL/min after, accessed at insect population single from connecing
Mirid, cyrtorhinus adult.Record test Mirid, cyrtorhinus into stinkbug enter two selection arms times and direction, every test
Mirid, cyrtorhinus observe 5 minutes into stinkbug, and the Mirid, cyrtorhinus of test are moved to more than any support arm 3cm, and in support arm 3cm into stinkbug
Duration afterwards calculates effectively selection more than 1min.If not moved always in 5min and being then designated as reactionless test worm, data are not counted in.
Data carry out Chi-square Test using spss16.0.
Result of the test such as Figure 11:Concentration be 50ppm under, trans-China pink it is dilute to Xiong shoulder green plant bug have significantly into stinkbug
Attractant effect, lure rate up to 36.67%, P is male=0.041, X2=4.174;To Ci shoulder green plant bug lure rate into stinkbug
Up to 16.67%, X2=0.839, P=0.36.Thus results presumption it is trans-the dilute Xia of the environment indoors Dui of China pink shoulder green plant bug into
Stinkbug has significant attractant effect.
Reference picture 4,5,6, is tested with the dilute solarium for luring Mirid, cyrtorhinus of trans-China pink.
Selection is without strong wind fine day morning 7:30-10:30 and afternoon 4:30-7:30 carry out solarium's examination in Hua Zhong Agriculture University
Test.The rice seedlings (see Fig. 3) in 6 basin tillering stages are put into the 100 mesh sarongs of 1m*1m*0.8m, bread is put into intermediate blank area
Box makes sarong bottom level consistent with gauze.10000ppm is dissolved in absolute ethyl alcohol by trans-China pink is dilute, it is dilute with paraffin oil
Release to 50ppm, 158ppm, 500ppm, 1580ppm, 5000ppm this 5 concentration are tested.40 μ l are added in 1g lanolin
Trans-the China pink of certain concentration is dilute, using paraffin oil as control, is uniformly applied on the rectangle filter paper of 2.5*7cm, uses
Safety pin is fixed in rice seedlings stalk, and fixed position is apart from ground 10cm.A middle basin rice seedlings put 4 filter papers, both sides point
Fang Zhi 2.If being put into 50 4-5 age Mirid, cyrtorhinus stinkbugs sarong center is careful.Each basin rice seedlings are recorded after 3 hours
If on Mirid, cyrtorhinus stinkbug number, a concentration is tested in each sarong, 5 concentration complete on the same day, in triplicate.Experiment
Result is shown in Figure 12.
Data result is analyzed with the Chi-square Test of spss16.0.
Through interpretation of result, the dilute , Dui when concentration is 50ppm of trans-China pink shoulder green plant bug have significant attractant effect (X2
=5.513, P=0.019), lure rate up to 42.84%;When concentration is 158ppm , Dui shoulder green plant bug have and extremely significantly lure
Effect (X2=47.79, P=0.001), lure rate up to 47.79%;Concentration be 500ppm when, trans-China pink Xi Dui shoulder it is green
Fleahopper has extremely significant attracting action (X2=13.476, P=0.001), lure rate up to 47.16%;It is 1580ppm in concentration
When, trans-China pink Xi Dui shoulder green plant bug lure rate up to 20.52% (X2=1.295, P=0.255);It is in concentration
During 5000ppm , Dui attractant effect of the shoulder green plant bug in solarium reach most preferably (P<0.01, X2=24.739), lure rate to surpass
50% is crossed, up to 65.84%.
This experimental result provides the application method with feasibility for field test, and trans-China pink is dilute in concentration
Attractant effect is optimal during for 5000ppm, there is provided the concentration and quality foundation of field test.
With the dilute field test for luring Mirid, cyrtorhinus of trans-China pink.
In Hubei Province, the Suizhou City county trans-China pink of the completion of in October, 2016 No. 1 of in August, 2016 No. 27 to that lives in peace is dilute to shoulders
Green plant bug lures experiment.Confirm before dispenser Tian Jian shoulder green plant bug be mainly into stinkbug.Trans-the China pink of release is dilute and compares paraffin
The field of oil is repeated 4 times, and each plot area is 64m2(8*8m), each minizone sets the protection row of a width of 1m.Cell is distributed
As shown in Figure 7.
Position hangs two lures as shown in Figure 8 between in the cell.Lure prepares:Trans-China pink is dissolved with paraffin oil
It is dilute to concentration 10000ppm.Uniformly it is applied at the mineral water bottle bottom interior wall of 600ml with lanolin, and is opened herein some
The circular hole of a diameter of 1cm.In order to prevent rainwash and increase the volatilization efficiency of attractant, 3 are prepared on mineral water bottle top
Individual " small window ".The mineral water bottle that will be prepared is fixed in rice terrace with 80cm bamboo poles, mineral water bottle is located at paddy rice cane.
Two lures are placed in the middle of each cell, distance is 4m between two lures (see Fig. 9).
The 2nd, 4,8,16, the 32 day all directions four direction in lure claps method sampling using basin after administration, such as Fig. 9 institutes
Show.Each direction takes 3 pocket rice seedlings, Tiao Cha shoulder green plant bug into stinkbug, if the quantity of stinkbug and brown paddy plant hopper.
Carried out with SPSS16.0 One-way ANOVE analysis dispenser field and control Tian shoulder green plant bug and the pocket worm of brown paddy plant hopper hundred
Difference between amount.
Experimental result as shown in Figure 13, Figure 14, at the 4th day, trans-China pink Xi Dui shoulder green plant bug have extremely notable into stinkbug
Attracting action (P=0.028).In other times for sampling, the dilute field Zhong of trans-China pink shoulder green plant bug it is equal into stinkbug value
Higher than control field, but You Yu shoulder green plant bug lifetime, at the 8-16 days Mirid, cyrtorhinus mainly with Mirid, cyrtorhinus
If the form of stinkbug is present.And at the 32nd day, due to have arrived at Shi months shoulder green plant bug sprout wings migrate, insect population reduction,
So do not have significant difference.If Er shoulder green plant bug stinkbug in each sample time, hundred pocket worm amounts are above control group, and have increasing
Plus trend, if show trans-China pink Xi Dui shoulder green plant bug stinkbug have attracting action, simply because field has different not show
Significant difference.
Experimental result as shown in figure 15, if apply trans-China pink dilute field Zhong , shoulder green plant bug stinkbug 32 days total hundred pockets
Worm amount has significant difference (P=0.022) with field is compareed, and lures rate up to 37.161%.Qie shoulder green plant bug into 32 days of stinkbug
Total hundred pockets worm amount also with compare field and have significant difference (P=0.048), lure rate up to 20.741%.
Claims (5)
1. the dilute Zuo Wei of trans-China pink shoulder green plant bug attractant application, it is characterised in that:Trans-China pink is dilute in absolute ethyl alcohol
In be dissolved to 10000ppm, when with paraffin oil or n-hexane, to be diluted to concentration be 10ppm -10000ppm, Zuo Wei shoulder green plant bug
Attractant.
2. dilute Zuo Wei of trans-China pink according to claims 1 shoulder green plant bug attractant application, it is characterised in that:
Trans-China pink is dilute to be dissolved to 10000ppm in absolute ethyl alcohol, is diluted with n-hexane, conduct when concentration is 10ppm -1250ppm
shoulder green plant bug attractant.
3. dilute Zuo Wei of trans-China pink according to claims 1 or 2 shoulder green plant bug attractant application, its feature exists
In:Trans-China pink is dilute to be dissolved to 10000ppm in absolute ethyl alcohol, is diluted with n-hexane, when concentration is 50ppm Zuo Wei shoulder it is green
If fleahopper stinkbug attractant.
4. dilute Zuo Wei of trans-China pink according to claims 1 or 2 shoulder green plant bug attractant application, its feature exists
In:Trans-China pink is dilute to be dissolved to 10000ppm in absolute ethyl alcohol, is diluted with paraffin oil, when concentration is 50ppm Zuo Wei shoulder it is green
Fleahopper both sexes are into stinkbug attractant.
5. dilute Zuo Wei of trans-China pink according to claims 1 or 2 shoulder green plant bug attractant application, its feature exists
In:Trans-China pink is dilute be dissolved in paraffin oil concentration for 10000ppm be applied to field Yin You shoulder green plant bug.
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CN110367253A (en) * | 2019-08-08 | 2019-10-25 | 福建省农业科学院植物保护研究所 | A kind of inducing agent of planthopper predator Mirid, cyrtorhinus |
CN111141874A (en) * | 2019-03-27 | 2020-05-12 | 广西壮族自治区植保站 | Method for measuring trapping and killing effect of bactrocera dorsalis adults |
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