CN105191935B - A kind of longicorn beetle imago attractant - Google Patents

A kind of longicorn beetle imago attractant Download PDF

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Publication number
CN105191935B
CN105191935B CN201510718829.XA CN201510718829A CN105191935B CN 105191935 B CN105191935 B CN 105191935B CN 201510718829 A CN201510718829 A CN 201510718829A CN 105191935 B CN105191935 B CN 105191935B
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linalool
aldehyde
longicorn beetle
cis
carypohyllene
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CN105191935A (en
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樊建庭
朱宁
张冬勇
谢苹
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Zhejiang A&F University ZAFU
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Zhejiang A&F University ZAFU
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Abstract

A kind of longicorn beetle imago attractant, belongs to forestry pest technical field.It is made up of along the alcohol of 3 hexene 1,98%a farnesenes 95% aldehyde C-9,98% linalool, 90% β carypohyllenes, 98%, 95% aldehyde C-9:98% linalool:90% β carypohyllenes:98% along the alcohol of 3 hexene 1:The volume ratio of 98%a farnesenes is 15:1‑5:1‑10:1‑5:1‑5.A kind of above-mentioned longicorn beetle imago attractant; reasonable mixture ratio; attractant effect is notable; have the advantages that efficient, environmentally friendly, safety, lasting period are long; the common trees such as willow, willow, oranges and tangerines, hickory nut, the Mount Huang Luan Shu endangered by longicorn beetle for protection are significant, can be widely applied to the fields such as shelter-forest, economic forest, afforestation woods and orchard.

Description

A kind of longicorn beetle imago attractant
Technical field
The invention belongs to forestry pest technical field, specially a kind of longicorn beetle imago attractant.
Background technology
Longicorn beetleAnoplophora chinensis(Forster)Belong to coleoptera Coleoptera Cerambycidaes Cerambycidae, English name Citus longhorned beetle, are widely distributed in China, endanger the one of more seeds Moth dryness insect is planted, with larva moth food trunk, causes a large amount of trees weak or even death.Longicorn beetle main host plants include willow, 19 sections 29 such as willow, oranges and tangerines, hickory nut, soapberry, Mount Huang Luan Shu, horse-tail belong to 48 kinds, coast protection forest to China, Economic forest and the serious threat of greening seedling industry composition.Europe found longicorn beetle in 2000 in North of Italy first.At present, Longicorn beetle is classified as important quarantine sex object by European plant protective tissue, brings serious to Chinese normal foreign export Influence.
At present, prevention and controls mainly have manual catching and killing, chemical prevention and Window maintenance to utilize, but longicorn is class protection The more perfect insect of mechanism, particularly larva hidden, channel of living are complicated, and adult body wall, elytrum be thick and hard, drug-resistant capability By force, more long and uneven from generation to generation, grade various features make traditional chemical prevention be difficult to prove effective to fecundity by force.
Golden water etc. finds that China tree has stronger attracting action to longicorn beetle, lures distance up to more than 200m.It is molten with difference After agent immersion, lure is made, there is attracting action to longicorn beetle, but the experiment is to carry out in insect cage indoors, is entered in woodland Longicorn beetle is not lured in capable experiment.Yasui researchs think that longicorn beetle is by the information substance institute of injured host plant release Attract, be gathered near host plant.Additionally, it was found that the contact sex pheromone in longicorn beetle female adult wing base portion includes 8 carbon aquations Compound, 4 ketones and 3 lactone compounds.
The patent of invention " longicorn beetle attractant " of Xu Huachao et al., application number:201410030112.1, just in substantive examination. The invention provides a kind of longicorn beetle attractant, its raw material includes the mass ratio of laurene and ethanol, the laurene and ethanol Be 1: 1, the laurene, ethanol purity >=98%.But, we have discovered that, laurene is to cause longicorn beetle feeler A kind of common host's compound of potentiometric response, and in Field tests, 2 wheat harvesting period times, only trap to 2 longicorn beetles, its Result is difficult to reach the purpose of real monitoring and preventing and treating longicorn beetle.
Up to now, also without a kind of real effective longicorn beetle attractant product in production application.
The content of the invention
For the above-mentioned problems in the prior art, a kind of efficient, environmental protection, peace are provided it is an object of the invention to design Entirely, the technical scheme of lasting period longicorn beetle imago attractant long, the attractant effect of the attractant is notable.
A kind of described longicorn beetle imago attractant, it is characterised in that by 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α-farnesene composition, 95% aldehyde C-9:98% linalool:90% β-carypohyllene:98% cis- 3- oneself Alkene -1- alcohol:The volume ratio of 98% α-farnesene is 1-5:1-5:1-10:1-5:1-5.
A kind of described longicorn beetle imago attractant, it is characterised in that 95% aldehyde C-9:98% linalool:90% β-carypohyllene: 98% cis- blatter alcohol:The volume ratio of 98% α-farnesene is 2-4:2-4:2-8:2-4:2-4.
A kind of described longicorn beetle imago attractant, it is characterised in that 95% aldehyde C-9:98% linalool:90% β-carypohyllene: 98% cis- blatter alcohol:The volume ratio of 98% α-farnesene is 2.5-3.5:2.5-3.5:4-6:2.5-3.5:2.5-3.5.
A kind of described longicorn beetle imago attractant, it is characterised in that 95% aldehyde C-9:98% linalool:90% β-carypohyllene: 98% cis- blatter alcohol:The volume ratio of 98% α-farnesene is 3:3:5:3:3.
The application method of described a kind of longicorn beetle imago attractant, it is characterised in that comprise the following steps:
1)According to 95% aldehyde C-9:98% linalool:90% β-carypohyllene:98% cis- blatter alcohol:98% α-farnesene Volume ratio is 1-5:1-5:1-10:1-5:1-5 measures each material respectively, standby;
2)Respectively by step 1)In 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α-farnesene is added drop-wise on the carrier filter fiber in different sustained release bags;
3)Then each sustained release bag to be held together can be used for and lures longicorn beetle adult.
A kind of application method of described longicorn beetle imago attractant, it is characterised in that step 1)In:95% aldehyde C-9:98% virtue Camphor tree alcohol:90% β-carypohyllene:98% cis- blatter alcohol:The volume ratio of 98% α-farnesene is 2-4:2-4:2-8:2-4:2-4.
A kind of application method of described longicorn beetle imago attractant, it is characterised in that step 1)In:95% aldehyde C-9:98% virtue Camphor tree alcohol:90% β-carypohyllene:98% cis- blatter alcohol:The volume ratio of 98% α-farnesene is 2.5-3.5:2.5-3.5:4-6: 2.5-3.5:2.5-3.5.
Described aldehyde C-9, English name Nonanal, colourless to faint yellow oily transparency liquid.It is dilute in strong fat breath It is in orange and rose fragrance after releasing.185 DEG C or 93 DEG C of boiling point (3066Pa), 5~7 DEG C of fusing point, 71 DEG C of flash-point.It is dissolved in ethanol, third Glycol, most of nonvolatile oils and mineral oil, it is several insoluble in G & W.GB 2760-1996 are defined as temporarily allowing to use Flavorant.It is mainly used in preparing the type essence such as orange, lemon and limette.Can be used for food.
Described linalool, alias Linalool, English name Linalool belongs to chain terpene alcohols, there is α-and β-two kinds Isomers.Colourless liquid, with lily of the valley fragrance, but has different aromas with source.Proportion 0.865(15℃), boiling point 195-200 DEG C, specific rotation+19.30.Water is practically insoluble in, insoluble in glycerine.Be dissolved in propane diols, nonvolatile oil and mineral oil, it is miscible in Ethanol and ether.Index of refraction:1.4600~1.4640.The green calyx green grass or young crops base of a fruit of plant often contains such delicate fragrance.Left-handed partially sweet, dextrorotation is inclined Clearly, its delicate fragrance is mainly used, therefore dextrorotation fragrance is preferable.The linalool fragrance of separate sources has larger difference, and natural linalool is clear Fragrant hair thoroughly, but be frequently not single fragrance;Synthesis linalool fragrance is general more pure and mild.
Described β-carypohyllene, also known as β-Allyl-4,3-guaiacol, English name β-Caryophyllene, molecular weight is 204.36, its Proterties is colourless to micro- yellow oily liquids, with light cloves like fragrance.119-121 DEG C of boiling point (1466Pa).Be dissolved in ether and Ethanol, it is water insoluble.Natural product are present in caryophyllus oil, clove-stems oil, cassia zeylanicum oil, cinnamon leaves oil, lavender oil, thyme In the essential oils such as oil, papper oil, pimento oil.
Described cis- blatter alcohol, also known as leaf-alcohol, English name cis-3-Hexen-1-ol, colourless oil liquid have Strong fresh leafiness fragrance.Density 0.8505,156.5 DEG C of boiling point, refractive index 1.438,44 DEG C of flash-point is dissolved in ethanol, the third two Alcohol and most of nonvolatile oils, it is atomic to be dissolved in water.It is naturally occurring in citrus fruit juice, beans, perhaps brandy, various plants Leaf, essential oil and fruit in.
Described α-farnesene, also known as α-farnesene, English name α-Farnesene, molecular weight 204, colorless oil Liquid, there is the fragrance of a flower, blue or green fragrant and face cream fragrance.It is water insoluble, it is dissolved in the organic solvents such as ethanol.Boiling point():138-140 ºC (1.2kpa), flash-point 95-100 oC, relative density (0.812, refractive index 1.476.It is naturally occurring in orange oil, attar of rose, beer In hops, Java Cananga Oil.
Related in above-mentioned 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol and 98% α-farnesene And percentage composition refer to product purity.
Above-mentioned 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol and 98% α-farnesene From lark prestige, Science and Technology Ltd. directly buys.
A kind of above-mentioned longicorn beetle imago attractant, reasonable mixture ratio, attractant effect significantly, with it is efficient, environmentally friendly, safely, hold The common trees such as the advantages of effect phase is long, willow, willow, oranges and tangerines, hickory nut, the Mount Huang Luan Shu endangered by longicorn beetle for protection It is significant, can be in field extensive uses such as three Norths shelter-forest, coast protection forest, economic forest, urban afforestation woodss.This is Longicorn beetle imago attractant product is successfully developed first, has filled up domestic and international blank.
The content being related in present specification unless otherwise indicated, is volumn concentration.
Brief description of the drawings
Fig. 1 is different compounds to longicorn beetle woodland trap effect figure;
Fig. 2 is trap effect figure of the attractants that are made of embodiment 1-7 to longicorn beetle adult, and 1-7 is respectively embodiment 1- 7;
Fig. 3 is contrast trap effect figure of the different attractants to longicorn beetle adult, and the ZL in figure makes for the embodiment of the present invention 6 Into attractant embodiment 6, X is the patent Example of Xu Huachao et al.(The mass ratio of laurene and ethanol is 1: 1).
Specific embodiment
In conjunction with embodiments of the invention and contrast test, beneficial effects of the present invention are further illustrated.
Embodiment 1
1)Measure 95% aldehyde C-9 of 2ml, 98% linalool of 2ml, the 90% β-carypohyllene of 2ml, 2ml 98% cis- 3- hexenes- 98% α-the farnesene of 1- alcohol, 2ml, it is standby;
2)Respectively by step 1)In 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α-farnesene is added drop-wise on the carrier filter fiber in different sustained release bags;
3)Then each sustained release bag is held together, obtains final product longicorn beetle imago attractant.
Embodiment 2
1)Measure 95% aldehyde C-9 of 2ml, 98% linalool of 2ml, the 90% β-carypohyllene of 4ml, 4ml 98% cis- 3- hexenes- 98% α-the farnesene of 1- alcohol, 4ml, it is standby;
2)Respectively by step 1)In 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α-farnesene is added drop-wise on the carrier filter fiber in different sustained release bags;
3)Then each sustained release bag is held together, obtains final product longicorn beetle imago attractant.
Embodiment 3
1)Measure 95% aldehyde C-9 of 4ml, 98% linalool of 4ml, the 90% β-carypohyllene of 2ml, 2ml 98% cis- 3- hexenes- 98% α-the farnesene of 1- alcohol, 2ml, it is standby;
2)Respectively by step 1)In 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α-farnesene is added drop-wise on the carrier filter fiber in different sustained release bags;
3)Then each sustained release bag is held together, obtains final product longicorn beetle imago attractant.
Embodiment 4
1)Measure 95% aldehyde C-9 of 4ml, 98% linalool of 4ml, the 90% β-carypohyllene of 6ml, 6ml 98% cis- 3- hexenes- 98% α-the farnesene of 1- alcohol, 2ml, it is standby;
2)Respectively by step 1)In 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α-farnesene is added drop-wise on the carrier filter fiber in different sustained release bags;
3)Then each sustained release bag is held together, obtains final product longicorn beetle imago attractant.
Embodiment 5
1)Measure 95% aldehyde C-9 of 6ml, 98% linalool of 4ml, the 90% β-carypohyllene of 2ml, 4ml 98% cis- 3- hexenes- 98% α-the farnesene of 1- alcohol, 6ml, it is standby;
2)Respectively by step 1)In 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α-farnesene is added drop-wise on the carrier filter fiber in different sustained release bags;
3)Then each sustained release bag is held together, obtains final product longicorn beetle imago attractant.
Embodiment 6
1)Measure 95% aldehyde C-9 of 2ml, 98% linalool of 4ml, the 90% β-carypohyllene of 6ml, 4ml 98% cis- 3- hexenes- 98% α-the farnesene of 1- alcohol, 2ml, it is standby;
2)Respectively by step 1)In 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α-farnesene is added drop-wise on the carrier filter fiber in different sustained release bags;
3)Then each sustained release bag is held together, obtains final product longicorn beetle imago attractant.
Embodiment 7
1)Measure 95% aldehyde C-9 of 6ml, 98% linalool of 4ml, the 90% β-carypohyllene of 2ml, 2ml 98% cis- 3- hexenes- 98% α-the farnesene of 1- alcohol, 2ml, it is standby;
2)Respectively by step 1)In 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α-farnesene is added drop-wise on the carrier filter fiber in different sustained release bags;
3)Then each sustained release bag is held together, obtains final product longicorn beetle imago attractant.
Beneficial effects of the present invention are further illustrated below by way of experiment
Described in the step of screening key component following steps:Insect volatile matter is collected with atmosphere sampling instrument, gaseous mass spectrum is used Combined instrument analyzes insect compound, and key compound is screened with the chromatograph joint used instrument of tentaculum electric potential, and field activity is carried out with trapper Experiment, finally filters out key component.
Experiment one:The main volatile constituent analysis of host plant
Test method
The volatile gas of host plant branch are collected using Dynamic headspace bagging method.Use polyethylene plastic bag (Reynolds, Richmond, VA)Set wraps up in branch position, by the glass tube containing Supelco adsorbents(7 cm × 0.5 cm ID)Air inlet with QC-1S atmosphere sampling instruments is connected.And it is filled with the glass tube of activated carbon(7 cm × 1 cm ID)Then it is connected with the gas outlet of atmosphere sampling instrument, all parts of sample devices are connected by silicone tube.The flow velocity of airometer 1.5 l/min are maintained, the sampling time is 5 h(10:00-15:00), each treatment is repeated 3 times.Hickory nut, Chinese wax are extracted respectively With the shoot of Mount Huang Luan Shu.The sample of collection is with containing with n-dodecane(150bbp)It is interior target chromatographically pure n-hexane (3x500ul)Solvent is eluted, and it is stand-by that elution samples are stored in subzero -5 DEG C of refrigerators.GC-MS conditions:Splitless injecting samples, enter every time Sample 2ul.Carrier gas is 99.999% high-purity helium, flow velocity 1ml/min.Heating schedule:It is initially 50 DEG C, constant temperature 1min, with 8 DEG C/speed of min is warmed up to 250 DEG C, keeps 6min, and 32min is run altogether.Solvent delay 3min.230 DEG C of ion source temperature, four 150 DEG C of level bar temperature.Detector temperature is 280 DEG C, and preceding injector temperature is 220 DEG C, electron bombardment voltage 70eV, mass scanning Scope 30-500.
Result of the test is shown in Table 1.
Separating obtained volatile gas composition analysis result shows that Chinese wax branch predominant gas volatile identification goes out 16 Kind, Mount Huang Luan Shu branches have 17 kinds, and hickory nut branch has 20 kinds.Multiple components are present in the volatile gas of different host plants Significant difference(Table 1).Volatile quantity of the aldehyde C-9 in Chinese wax and Mount Huang Luan Shu is significantly higher than hickory nut branch.2- ethyls -1- oneself Alcohol is present in Chinese wax and Mount Huang Luan Shu simultaneously.Laurene, cis- 3- hexenols, decane, limonene and α-farnesene are only in mountain core Peach branch kind is detected.The volatile quantity of decane, acetophenone and hendecane in the Luan Shu of Mount Huang is significantly higher than Chinese wax and hickory nut.Camphane Alkene is significantly higher than Chinese wax branch in the performance amount of Mount Huang Luan Shu and hickory nut branch.Ocimenum is not detected in Chinese wax branch kind Arrive.Volatile quantity of the tridecane in hickory nut branch is significantly higher than Chinese wax and Mount Huang Luan Shu.Pentadecane is only examined in the Luan Shu of Mount Huang Measure, octadecane is not detected.
Experiment two:Longicorn beetle is reacted the GC-EAD of host's composition
Test method:The whole feeler of active preferably longicorn beetle adult is quickly cut by base portion, the excision of feeler tip A little, the feeler length about 2.5cm after treatment, feeler is transversely lapped on the two ends of the diverging electrodes for speckling with conducting resinl in advance, is touched Angle two ends are immersed in conducting resinl, finally by diverging electrodes insertion EAG probes.Chinese wax, Mount Huang Luan Shu and mountain core are tested respectively Reaction of the peach branch eluent to longicorn beetle adult feeler, using Splitless injecting-Sample, 220 DEG C of injector temperature, detector temperature 280℃.Each sample introduction 2ul is repeated 5 times to GC-EAD analyses are carried out in gas chromatograph.Heating schedule condition:Initial temperature is 50 DEG C, 1min is kept, 250 DEG C are risen to the speed of 8 DEG C/min, keep 6min, run 32 minutes altogether.On GC-EAD chromatograms Compound peak shape and retention time carry out detailed comparisons, so as to identify active material.
Result of the test shows:According to the collection of illustrative plates that 5 repetitions are tested, selection is one of to stimulate more substantially and with repetition The collection of illustrative plates of reaction, longicorn beetle adult has IR to volatile materials aldehyde C-9, cis -3- hexenols and α-farnesene.
Experiment three:Different compounds are to anoplophora glabripennis woodland trap effect preliminary examination
Test method:The active ingredient analyzed in Main Ingredients and Appearance and GC-EAD that will be analyzed in host plant, Yi Jiwen Offering the related compounds of document announcement carries out the trapping experiments of woodland.
Attractant composition is sustained bag (2.5 cm × 2.5 cm × 120 μm thickness) release using polyethylene.According to examination Design is tested, every kind of attractant composition 2ml is added drop-wise to the carrier filter fiber in sustained release bag respectively(15×6 mm)On, every kind of place Reason sets 5 trappers, and attractant is changed once for every 45 days.Using cross type Longicorn trap, on adjacent two trees A waddy is bound, trapper is hung on waddy, trapper is highly about 2 m, is hung up on trapper and lured equipped with longicorn beetle The polyethylene sustained release bag of agent composition, is separated by about 30 meters between adjacent trapper.Result is once traped in inspection per week, in detail record Species, quantity, sex of longicorn etc..Described polyethylene sustained release bag prompt four directions Science and Technology Ltd. can be bought from Beijing.
Result of the test(See Fig. 1)Show:In single compound, aldehyde C-9, linalool, β-carypohyllene, cis- 3- hexenes -1- Alcohol and α-farnesene have all traped longicorn beetle, and wherein β-carypohyllene trap effect is best, aldehyde C-9, linalool, and cis- 3- hexenes- 1- alcohol and α-farnesene trap effect are secondly;And 3- carenes, hexanal, trans- 2- hexenoic aldehydes, trans- 2- hexenols, cis- 2- amylenes Alcohol, 1-POL, 3- amylalcohols, eudesmol, benzaldehyde, phenylacetaldehyde and (E) -2- octenals all do not trap longicorn beetle.
Experiment four:The woodland trapping experiments of attractant composition different ratio
Test method:Aldehyde C-9, linalool, β-carypohyllene, five kinds of cis- blatter alcohol and α-farnesene are effectively drawn Lure composition is tested according to different ratio, tests its trap effect, and other test methods are with experiment three.
Result of the test(See Fig. 2)Show:95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol and 98% α-farnesene is 1:2:3:2:In the case of 1(That is embodiment 6), its trap effect preferably, embodiment 1~5, embodiment 7 Also there is good trap effect.
Experiment five:From the woodland contrast test of different attractants
Test method:By this patent embodiment 6 and other reported that attractant composition carries out Field tests, comparative analysis its To the trap effect of longicorn beetle, other test methods are with three, of experiment
Result of the test(See Fig. 3)Show:This patent embodiment 6 can trap a large amount of longicorn beetles, be significantly higher than other Attractant, identical experiment is carried out with embodiment 1-5 and 7, can also reach beneficial effect of the present invention.
Applicant on the basis of a large amount of host plant compounds, successfully filters out a kind of longicorn beetle by studying for many years Imago attractant embodiment, has the advantages that efficient, safe and environment-friendly, long using the simple, lasting period, its cooperation Longicorn trap Hang on woodland, can monitor and trap and kill longicorn beetle, can be widely applied for be subject to longicorn beetle harm oranges and tangerines, willow, willow, The shelter-forests such as Mount Huang Luan Shu, soapberry and the greening woods, with wide popularizing application prospect.

Claims (5)

1. a kind of longicorn beetle imago attractant, it is characterised in that by 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- 3- Hexen-1-ol, 98% α-farnesene composition, 95% aldehyde C-9:98% linalool:90% β-carypohyllene:98% cis- blatter alcohol: The volume ratio of 98% α-farnesene is 1-3:2:1-3:2-3:1-3.
2. a kind of longicorn beetle imago attractant as claimed in claim 1, it is characterised in that 95% aldehyde C-9:98% linalool:90%β- Carypohyllene:98% cis- blatter alcohol:The volume ratio of 98% α-farnesene is 2:2:3:3:1.
3. a kind of longicorn beetle imago attractant as claimed in claim 1, it is characterised in that 95% aldehyde C-9:98% linalool:90%β- Carypohyllene:98% cis- blatter alcohol:The volume ratio of 98% α-farnesene is 3:2:1:2:3.
4. a kind of longicorn beetle imago attractant as claimed in claim 1, it is characterised in that 95% aldehyde C-9:98% linalool:90%β- Carypohyllene:98% cis- blatter alcohol:The volume ratio of 98% α-farnesene is 1:2:3:2:1.
5. a kind of application method of longicorn beetle imago attractant as claimed in claim 1, it is characterised in that comprise the following steps:
1)95% aldehyde C-9 is measured according to volume ratio:98% linalool:90% β-carypohyllene:98% cis- blatter alcohol:98% α-method Alkene, it is standby;
2)Respectively by step 1)In 95% aldehyde C-9,98% linalool, 90% β-carypohyllene, 98% cis- blatter alcohol, 98% α- Farnesene is added drop-wise on the carrier filter fiber in different sustained release bags;
3)Then each sustained release bag to be held together can be used for and lures longicorn beetle adult.
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