CN112544636B - Application of Daniu medicine in killing mite and pest, its biological control medicine and preparation method - Google Patents
Application of Daniu medicine in killing mite and pest, its biological control medicine and preparation method Download PDFInfo
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- CN112544636B CN112544636B CN202011516249.XA CN202011516249A CN112544636B CN 112544636 B CN112544636 B CN 112544636B CN 202011516249 A CN202011516249 A CN 202011516249A CN 112544636 B CN112544636 B CN 112544636B
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- mites
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to application of Daniu insecticide in the aspects of mite killing and insect killing, a biological control medicament and a preparation method thereof, and belongs to the field of biological control. The invention relates to application of a Daniiang in mite killing and insect killing, and a biological control medicament based on Daniiang essential oil. The biological control medicament can efficiently kill harmful mites and pests, and is non-toxic, harmless, safe, reliable and low in cost.
Description
Technical Field
The invention belongs to the field of biological control, and particularly relates to application of Daniu insecticide in the aspects of mite killing and insect killing, a biological control medicament and a preparation method thereof.
Background
Tetranychus urticae (Tetranychus urticae) is a worldwide pest mite and belongs to the class of Arachnida. The host plants of the tetranychus urticae are very wide, more than 800 plants of more than 50 families, and the common plants comprise various crops and nearly hundreds of weeds, such as strawberries, eggplants, cucumbers, tomatoes, hot peppers, soybeans, cowpeas, kidney beans, sorghum, watermelons, cucumbers, peaches, pears, apples, apricots, plums, cherries, geraniums, poinsettia, cotton and the like. The two-spotted spider mites and the nymph mites can all be harmed, the initial harmful mites mostly gather on two sides of the vein of the dorsum of the leaves, two sides of the vein of the front of the affected leaves show green loss, the leaves quickly lose green and become brown along with the increase of the number of the mites and the aggravation of the damage degree, the leaves gradually become hard and brittle, and finally become withered and yellow and fall off. The pesticide for preventing and controlling the tetranychus urticae mainly depends on applying a chemosynthetic acaricide, and because of long-term use, the tetranychus urticae has higher resistance to various common acaricides such as abamectin, pyridaben, fenpyroximate, thiamite and the like at present, and the recommended dose is basically ineffective. Meanwhile, the use of chemical agents also has the killing effect on non-target organisms, destroys the ecological balance and causes the pest mites to be rampant. The discovery of high-efficiency and environment-friendly acaricides is urgently needed.
Frankliniella occidentalis (Frankliniella occidentalis) belongs to Thysanoptera thrips, is a worldwide dangerous pest, has wide host range and is a food impurity, host plants comprise more than 500 important economic crops and food crops such as potatoes, eggplants, hot peppers, cucumbers, flowers, bananas, mangoes and the like, and the host types of Frankliniella occidentalis are continuously increased along with the continuous diffusion and spread of Frankliniella occidentalis. The Frankliniella occidentalis eats stems, leaves, flowers and fruits of plants by a rasping-sucking mouthpart, so that petals are discolored, leaves are shrunk, the stems and the fruits form scars, and finally, the plants are withered, and various plant viruses including tomato spotted wilt virus are spread, so that the agricultural production is seriously threatened. The chemical prevention and control method is still a main prevention and control means because the western flower thrips are small in size and secret in harm activities, parthenogenesis can be carried out, the reproductive capacity is strong, the population growth is rapid, but the long-term unreasonable use of chemical pesticides causes that the western flower thrips generate different degrees of drug resistance to insecticides such as organophosphorus, carbamate, pyrethroid and spinosad, so that the prevention and control difficulty is increased, and the pressure on the environment is increased. The discovery of green nuisanceless pesticide effective on frankliniella occidentalis is urgently needed in the production.
The louse prey (lipopeleis bostrychophilus) is an important storage pest belonging to the class of the insecta, order of rodentia. The psylla convolvulus can live among grains, plants, under the bark of trees, on lichens and old trees, is parthenogenesis, has tiny individuals, short development period, long service life of adults, poor appetite, strong hunger resistance and extremely high propagation speed, can cause serious economic loss when occurring in large quantity, has great harm to stored materials such as grains, foods, feeds, medicinal materials and the like, and can bring threat to the health of human beings because excrement adhered to the body of the psylla convolvulus can carry and propagate germs and cause allergy and has extremely high mobility. In actual production, storage class pest uses chemical fumigant prevention and cure more, and long-term single use hydrogen phosphide fumigant leads to the book louse of growing to the resistance to drug of hydrogen phosphide reinforcing, and easily causes the pest rampant again, can cause harm to human health even, seeks novel safe environmental protection's insecticide substitute and is the problem that needs solve urgently.
The radix Stephaniae Cepharanthae is root of Inula pterocarpum (Inula pterocarpus Franch.) of Compositae, and is a specific plant in China. Perennial herbs or shrubs, about 60-100 centimeters in height. The leaf stem of the inula cappa is upright, and the lower part of the inula cappa is woody. With thick woody roots. The leaves are grown alternately, the shape of the inverted cap is like a needle, the upward part of the cap is gradually narrowed, the length of the cap is about 13 cm, the width of the cap is about 4cm, the tip of the cap is blunt or round, the base of the cap is in a narrow wedge shape, the edge of the cap is provided with non-obvious thorn-shaped teeth, and two surfaces of the cap are roughened; the petiole has wings and extends downwards to form a wing stem. Head-shaped inflorescence arranged in a cone shape, apical growth and axillary growth; the general bract is in a bell shape and is provided with a plurality of lines of bracts in a linear shape; the flower is light yellow, the lingulate flower is narrow and thin, and the tubular flower is in multiple rows. The lean fruit has white crown hair. It is born in the shady and wet places of hillside, shrub and forest at the elevation of 2000-2800 m. Mainly produced in the south and west of Sichuan, north of Yunnan and northwest. The Chinese medicinal materials are bitter and mild. Clearing away heat and toxic material, relieving cough, eliminating phlegm, tonifying deficiency, dredging channels and collaterals, promoting diuresis and removing dampness. Can be used for treating dizziness, cardiopalmus, tinnitus, sweating due to debility, common cold, cough, carbuncle, ulcer, toxic swelling, and bone tuberculosis. The application of the large black insecticide in the aspects of killing mites and insects has not been reported in the field.
Disclosure of Invention
The invention is based on the blank in the field, finds the effect of the large black pesticide on killing tetranychus urticae, agricultural pest thrips occidentalis and storage pest psyllid, and provides the application of the large black pesticide in killing mites and pests in the field of biological control.
The technical scheme of the invention is as follows:
the application of Daniu medicine in killing mite and pest is disclosed.
The insects are selected from agricultural pests or storage pests; the mite is a pest mite;
preferably, the pest mites are tetranychus urticae;
preferably, the agricultural pest is frankliniella occidentalis, and the storage pest is booklice.
The acaricidal and insecticidal mode is selected from contact killing or smoking killing.
The big black medicine essential oil is adopted to kill mites and insects;
preferably, the Danigella damascena essential oil is extracted from a traditional Chinese medicine Danigella damascena.
The use of Daniu medicine in preparing biocontrol medicine.
A biological control medicament is characterized in that the active ingredient of the biological control medicament is Daniiang essential oil.
The biological control agent is selected from acaricides, and/or insecticides;
preferably, the acaricidal and insecticidal active ingredients of the big black drug essential oil comprise: carvacrol, thymol isobutyl ester and thymol isoamyl ester.
A process for preparing the biological control chemical includes such steps as decocting Danig medicine in water or distilling, condensing and extracting.
The water boiling refers to boiling treatment with water for 8-12h, preferably 10 h;
preferably, the distillation refers to steam distillation for 8-12h, preferably 10 h;
preferably, the extraction refers to extraction with n-hexane;
preferably, the temperature of the water bath for extraction is 38-45 ℃, preferably 40 ℃;
preferably, the mixture obtained by extraction is dried and then n-hexane is removed by evaporation;
preferably, the drying refers to drying with anhydrous sodium sulfate;
preferably, the evaporation refers to evaporation with a rotary evaporator.
The invention discovers the insecticidal and acaricidal effects of the major jettison, and verifies the outstanding effect of the major jettison in the field of biological control, so the invention requests to protect the application of the major jettison in killing mites and insects, the application of the major jettison in preparing acaricidal products, the application of the major jettison essential oil in controlling agricultural pests, the application of the major jettison essential oil in controlling storage pests, and the preparation of the major jettison essential oil takes the traditional Chinese medicine major jettison as a raw material, and the major jettison essential oil is prepared by the steps of boiling/distilling, condensing, extracting, dehydrating and evaporating a solvent, and the application of the major jettison essential oil in killing mites and insects. The invention also provides a biological control medicament based on the major dark green essential oil, which can efficiently kill harmful mites and pests, is nontoxic and harmless, does not produce pollution, and is a novel insecticide/acaricide which is safe, reliable, low in cost, environment-friendly and efficient.
Detailed Description
The present invention will be described in further detail with reference to the following specific examples, which should not be construed as limiting the scope of the present invention.
Sources of biological material
The source of the test insects is as follows: the frankliniella occidentalis, tetranychus urticae and the ticks are artificially bred.
The major dark drugs are traditional Chinese medicinal materials and purchased from a cloud drug net.
The reagents used in the experimental examples of the present invention, the test reagents not specifically described, were all reagents conventional in the art or obtained by methods conventional in the art, and were commercially available as analytical reagents. Unless otherwise specified, the experimental procedures carried out in the experimental examples are all those routine in the art.
Group 1 example, New use of Daniu drug for biological control
The group of embodiments provides the application of the large black insecticide in the aspects of killing mites and insects.
In particular embodiments, the pest is selected from a pest mite or pest;
in more specific embodiments, the pest mite is tetranychus urticae;
in a preferred embodiment, the agricultural pest is selected from thrips occidentalis;
in a preferred embodiment, the stored pests are selected from the group consisting of psyllids.
In some embodiments, the control refers to killing;
in other embodiments, the mode of action on tetranychus urticae and thrips occidentalis is contact killing and the mode of action on psyllid is fumigation.
In a further embodiment, the large black fungus essential oil is used for killing mites and insects;
preferably, the Danigella damascena essential oil is prepared by using a Danigella damascena as a raw material and adopting a distillation method.
Group 2 examples biocontrol pharmaceutical use of Danigella sativa
The present group of embodiments provides the use of a macrorrhiz in the preparation of a biocontrol agent.
In particular embodiments, the biocontrol agents include, but are not limited to: insecticides, acaricides.
More specifically, the biocontrol agents may be formulated into dosage forms conventional in the art, including but not limited to: powders, wettable powders, drops, suspension concentrates, emulsifiable concentrates, sprays, aerosols, baits, mosquito-repellent incense, sticking agents, smoke agents, insecticidal coatings and the like. Group 3 examples of biocontrol agents of the invention
The present group of embodiments provides a biological control agent. All embodiments of this group share the following common features: the active component of the biological control medicament is Daniiang essential oil.
In particular embodiments, the dosage form of the biocontrol agent is selected from a miticide or an insecticide;
preferably, the active ingredients of the essential oil of the great blackfungus comprise
In further embodiments, the biocontrol agent further comprises a pesticide art-acceptable adjuvant common in the art, including but not limited to: emulsifiers, organic solvents, fillers, wetting agents, diluents, and the like.
Group 4 examples preparation of the biocontrol agents of the present invention
The present group of embodiments provides a method of preparing a biological control agent. All embodiments of this group share the following common features: the preparation method comprises the following steps: decocting Daniu medicine in water or distilling, condensing and extracting.
In a particular embodiment, the distillation is steam distillation for 8-12h, preferably 10h, to extract substantially the volatile components of the plant material.
The boiling with water is carried out for 8-12h, preferably 10h, so as to boil essential oil components from plants completely.
Preferably, the extraction refers to extraction with n-hexane;
more specifically, using a 2L separatory funnel, n-hexane: and (3) obtaining a distillate of 1:1, and reserving an n-hexane layer in which the essential oil is dissolved after layering.
Preferably, the temperature of the extraction water bath is 38-45 ℃, preferably 40 ℃, and n-hexane can be quickly evaporated, condensed and recycled at the temperature.
Preferably, the mixture obtained by extraction is dried and then n-hexane is removed by evaporation;
preferably, the drying refers to drying with anhydrous sodium sulfate;
preferably, the evaporation refers to evaporation with a rotary evaporator.
Preferably, the specific gravity of the essential oil is 0.926 g/mL.
Experimental example 1 preparation of essential oil of Danigella damascena
3kg of Danig Yam is purchased from Yunyao medicinal net, treated with water boiling method for 8-10 h, the obtained oil-water mixture is condensed and recovered, extracted with n-hexane, dried with anhydrous sodium sulfate, and the n-hexane is removed by rotary evaporator to obtain the component of Danig Yam essential oil. Wherein the water bath temperature is 40 ℃.
The yield of the Daniuzheng essential oil is 0.60% (w/w), namely 0.6g of the Daniuzheng essential oil can be extracted from 100g of the Daniuzheng.
Experimental example 2 identification of essential oil components of Danigella sativa
Identifying the components of the major blackberry lily essential oil by adopting a GC-MS detection method:
a detection instrument: a gas chromatograph (Agilent6890N) and a mass spectrometer (Agilent 5973N). .
Detection conditions are as follows: HP-5MS30mm × 0.25mm × 0.25 μm quartz capillary column, initial column temperature 60 deg.C, keeping for 1min, programming to 180 deg.C at a rate of 12 deg.C/min, keeping for 0.5min, programming to 280 deg.C at a rate of 25 deg.C/min, keeping for 18min, vaporizer temperature 280 deg.C, carrier gas is helium, column flow is 1.0mL/min, and split ratio is 1: 10.
The ionization mode EI of the mass spectrum has the electron bombardment energy of 70eV, the scanning mass range of 20-550m/z and the scanning time of 2 s.
Sampling 1 mu L of Danigella damascena essential oil (100 times of acetone diluent), analyzing and identifying by a gas chromatography-mass spectrometer, searching a Nist05 standard mass spectrum library by an HP-MSD chemical workstation, and detecting and identifying 20 peaks by combining related documents.
The ingredient identification results are shown in table 1 below:
TABLE 1
The total percentage of all the components identified in Table 1 above is less than 100% because the essential oil of Hexaparia henryi still contains unknown components, which may be new compounds and cannot be identified by the existing GC-MS detection method.
Experimental example 3 acaricidal Effect of the biological control agent of the present invention
Tetranychus urticae-poisoning: LD50＝11.45mg/mL(10.70，12.22)
The killing effect verification of the major dark herb essential oil is carried out on tetranychus urticae by adopting a leaf disc method, and the specific operation is as follows: coating polytetrafluoroethylene on the inner wall of the culture plate, and drying by electric air blowing; heating water and agar at a ratio of 100:1.5 in a microwave oven, mixing, pouring into 12-well cell culture plate (2-3 ml per well), and cooling to solidify; taking fresh kidney bean leaves without pesticide, and punching the leaves by using a puncher with the diameter of 19mm to prepare a circular leaf disc; dissolving a certain amount of radix Dactylicapni essential oil with anhydrous ethanol (the amount is not more than 2% of the liquid medicine system), adding dimethyl sulfoxide (not more than 1% of the liquid medicine system) for assisting dissolution, preparing a mother solution, and diluting with distilled water to a required concentration to obtain the liquid medicine system; soaking the leaf disc in the agent for 10s, taking out, air drying, placing the kidney bean leaves soaked with the liquid medicine on a culture plate with agar poured therein with the back side of the kidney bean leaves upward, and inoculating 10 tetranychus urticae into each hole to form mites; firmly sticking the porous surface of the culture plate by rice paper, and putting the culture plate into an incubator at the temperature of 26-28 ℃, the relative humidity of 65-75% and the photoperiod L: D of 16: 8; record under the stereoscope after 24hThe number of dead people, i.e. the person who touches the touch screen with the writing brush is alive and the person who does not touch the touch screen is dead, is counted50。
Mortality (%) ═ death/test insect count 100
Corrected mortality (%) - (treatment-control mortality)/(1-control mortality) × 100
semi-Lethal Dose (LD)50): refers to the dose required to cause death in 50% of the subjects tested. Calculated by using Probit software.
The results are shown in table 2 below:
TABLE 2
LD5011.45mg/mL, 95% confidence interval (10.70, 12.22), y-6.98 +6.59 x. The meaning of this semilethal dose is: the drug solution system (including distilled water, solvent-alcohol and cosolvent-dimethyl sulfoxide) contains 11.45mg of essential oil per ml, and half of the tested individuals die after treatment at such concentration.
Experimental example 4 agricultural Pest-killing Effect of the biological control agent of the present invention
Frankliniella occidentalis-poisoning: LD50＝4.76mg/mL(4.39，5.17)
Frankliniella occidentalis-poisoning: dissolving a certain amount of radix Dactylicapni essential oil with anhydrous ethanol (amount is not more than 1% of the liquid medicine system), adding Tween 80 (not more than 0.5% of the liquid medicine system) for assisting dissolution, preparing a mother solution, and diluting with distilled water to a required concentration to obtain the liquid medicine system for later use; putting 1mL of liquid medicine into a glass scintillation bottle with the volume of 22mL, uniformly rolling for a plurality of circles to form a medicine film, pouring out the liquid medicine, and airing the scintillation bottle for later use. Taking fresh hyacinth beans without pesticide, preparing the hyacinth beans into a long section of 1cm square, immersing the hyacinth beans into the pesticide for 10s, taking out the hyacinth beans, airing the hyacinth beans, and placing the hyacinth beans in a scintillation bottle; setting 5 repetitions for each treatment, repeatedly inoculating second-instar nymphs of 10 Frankliniella occidentalis, and sealing the bottle mouth with a 150-mesh insect-proof net; and (5) placing the worm body at room temperature, observing the result after 24h, and stirring the worm body with a writing brush, wherein the worm body is recorded as dead after being immobilized.
Mortality (%) ═ death/test insect count 100
Corrected mortality (%) - (treatment-control mortality)/(1-control mortality) × 100
semi-Lethal Dose (LD)50): refers to the dose required to cause death in 50% of the subjects tested. Calculated by using Probit software.
The results are shown in Table 3:
TABLE 3
LD504.76mg/mL with a 95% confidence interval of (4.39, 5.17) and y-3.18 +4.69 x. The meaning of this semilethal dose is: each milliliter of the drug solution system (including distilled water, solvent-alcohol and cosolvent-Tween) contains mg of essential oil, and half of the test subjects die after treatment at such concentrations.
Experimental example 5 insecticidal Effect of the biological control agent of the present invention on storage pests
Psyllid-fumigation: LC (liquid Crystal)50＝0.38mg/L(0.36，0.40)
Fumigating activity of the great blackfungus essential oil on the lophatherum gracile is determined: picking 10 test insects with a brush pen, placing into a small glass bottle (easy to observe death condition of the test insects) with diameter of 0.5cm and height of 1.6cm, coating polytetrafluoroethylene on the inner wall of the bottle mouth to prevent the test insects from escaping, placing the small glass bottle into a large ground glass bottle (290mL) with height of 12cm and diameter of 4cm, taking acetone as solvent to dilute the medicament into an equal ratio series, and sucking 10 microliters of the medicament diluent with a pipette gun
Dropping the release liquid (6 concentrations) on a filter paper strip (7.5 cm in length and 1.5cm in width), adhering the filter paper strip in the ground bottle cap by using a solid adhesive, quickly screwing down the ground bottle cap, and winding a sealing film for a circle for sealing. After 24 hours, the death of the test insects was observed and recorded. Each concentration was repeated 5 times, each 10 replicates with no drug added to acetone as a blank. After 24 hours, observing, pouring the test insects in the small glass bottle on a piece of white paper, and stirring the bodies with a writing brush to ensure that the bodies die after being immobilized.
The mortality rate (%). is 100 x dead number/test number.
Corrected mortality (%) -100 × (treatment-control mortality)/(1-control mortality).
Semi-lethal dose (LC)50) I.e. the dose that caused 50% of the mortality of the psyllids, the semi-lethal dose was calculated using the Probit software package.
The results are shown in Table 4:
TABLE 4
LC500.38mg/L with a 95% confidence interval of (0.36, 0.40) and y 3.72+8.84 x. The meaning of this semilethal dose is: each liter of the enclosed space contains 0.38mg of essential oil, and half of the individuals to be tested die when treated at such a concentration.
Claims (21)
1. The application of the big black pesticide in agricultural acaricidal and insecticidal aspects;
the major jetiao is Asteraceae plant Eupatorium adenophorumInula pterocaula Root of franch.
2. Use of a cyazofamid as claimed in claim 1 in agriculture for killing mites and pests, where the mites are selected from the group consisting of mites; the pests are selected from agricultural pests, or, stored-grain pests.
3. Use of a tetranychus urticae as claimed in claim 2 in agricultural acaricidal killing, wherein the mites are selected from tetranychus urticae; the agricultural pests are selected from frankliniella occidentalis, or the storage pests are selected from booklice.
4. Use of a macrocarpium nigrum as claimed in any one of claims 1 to 3 in agriculture for killing acarids and insects, wherein the acarid killing and insect killing mode is selected from contact killing or smoking killing.
5. Use of the big black insecticide according to any one of claims 1 to 3, wherein the big black insecticide essential oil is used for killing mites and insects.
6. The use of the big black medicine in the aspects of agricultural acaricidal killing and insecticide according to claim 5, wherein the big black medicine essential oil is extracted from a traditional Chinese medicine big black medicine.
7. The use of the big black medicine in the aspect of preparing a biocontrol medicament, wherein the biocontrol refers to agricultural killing of mites and insects; the major jetiao is Asteraceae plant Eupatorium adenophorumInula pterocaula Root of franch.
8. A biological control agent is characterized in that the active ingredient of the biological control agent is Daniiang essential oil; the acaricidal and insecticidal active ingredients of the Daniiang essential oil comprise: carvacrol, thymol isobutyl ester, thymol isoamyl ester; the biological control refers to agricultural acaricidal and insecticidal; the major jetiao is Asteraceae plant Eupatorium adenophorumInula pterocaula Root of franch.
9. The biocontrol agent of claim 8, wherein said biocontrol agent is selected from the group consisting of acaricides, and/or insecticides.
10. A preparation method of biological control medicament is characterized in that the medicament is prepared by boiling or distilling, condensing, extracting and evaporating solvent of Danig drug;
the biological control refers to agricultural acaricidal and insecticidal; the major jetiao is Asteraceae plant Eupatorium adenophorumInula pterocaula Root of franch.
11. The process for preparing a biocontrol agent as claimed in claim 10, wherein said boiling with water is carried out for 8-12 hours.
12. The method of claim 11, wherein the boiling is carried out for 10 hours.
13. The process of claim 10, wherein the distillation is steam distillation for 8-12 h.
14. The process of claim 13, wherein the distillation is steam distillation for 10 hours.
15. The method of claim 10, wherein said extraction is performed with n-hexane.
16. The process of claim 15, wherein the temperature of the aqueous bath of the extraction is 38-45 ℃.
17. The process of claim 16, wherein the temperature of the aqueous bath used for extraction is 40 ℃.
18. The process for the preparation of a biocontrol agent as in any of claims 10 and 15-17 wherein the mixture obtained from the extraction is dried and then evaporated to remove n-hexane.
19. The process of claim 18, wherein drying is over anhydrous sodium sulfate.
20. The method of claim 10, wherein said evaporation is performed using a rotary evaporator.
21. The method of claim 18, wherein said evaporation is by rotary evaporation.
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