CN113367132A - Application of kidney bean volatile matter in attracting invading pest artichoke - Google Patents
Application of kidney bean volatile matter in attracting invading pest artichoke Download PDFInfo
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- CN113367132A CN113367132A CN202110444611.5A CN202110444611A CN113367132A CN 113367132 A CN113367132 A CN 113367132A CN 202110444611 A CN202110444611 A CN 202110444611A CN 113367132 A CN113367132 A CN 113367132A
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- attracting
- ethylbenzene
- frankliniella occidentalis
- squalene
- phthalate
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- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 29
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 25
- 244000019459 Cynara cardunculus Species 0.000 title claims description 5
- 235000019106 Cynara scolymus Nutrition 0.000 title claims description 5
- 235000016520 artichoke thistle Nutrition 0.000 title claims description 5
- 241000927584 Frankliniella occidentalis Species 0.000 claims abstract description 42
- YNQLUTRBYVCPMQ-UHFFFAOYSA-N Ethylbenzene Chemical compound CCC1=CC=CC=C1 YNQLUTRBYVCPMQ-UHFFFAOYSA-N 0.000 claims abstract description 34
- BJQHLKABXJIVAM-UHFFFAOYSA-N bis(2-ethylhexyl) phthalate Chemical group CCCCC(CC)COC(=O)C1=CC=CC=C1C(=O)OCC(CC)CCCC BJQHLKABXJIVAM-UHFFFAOYSA-N 0.000 claims abstract description 33
- YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000005667 attractant Substances 0.000 claims abstract description 14
- 230000031902 chemoattractant activity Effects 0.000 claims abstract description 14
- 241001414989 Thysanoptera Species 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims description 27
- 239000003039 volatile agent Substances 0.000 claims description 9
- 235000021332 kidney beans Nutrition 0.000 claims description 7
- 241000132536 Cirsium Species 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 19
- 235000013311 vegetables Nutrition 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- XOJVHLIYNSOZOO-SWOBOCGESA-N Arctiin Chemical compound C1=C(OC)C(OC)=CC=C1C[C@@H]1[C@@H](CC=2C=C(OC)C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)=CC=2)C(=O)OC1 XOJVHLIYNSOZOO-SWOBOCGESA-N 0.000 abstract 1
- MEMBJMDZWKVOTB-UHFFFAOYSA-N 1-ethyl-2,4-dimethylbenzene Chemical compound CCC1=CC=C(C)C=C1C MEMBJMDZWKVOTB-UHFFFAOYSA-N 0.000 description 16
- 238000001179 sorption measurement Methods 0.000 description 12
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 239000007789 gas Substances 0.000 description 8
- IVSZLXZYQVIEFR-UHFFFAOYSA-N m-xylene Chemical group CC1=CC=CC(C)=C1 IVSZLXZYQVIEFR-UHFFFAOYSA-N 0.000 description 8
- 230000006399 behavior Effects 0.000 description 7
- XCYJPXQACVEIOS-UHFFFAOYSA-N 1-isopropyl-3-methylbenzene Chemical compound CC(C)C1=CC=CC(C)=C1 XCYJPXQACVEIOS-UHFFFAOYSA-N 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 6
- QUEBYVKXYIKVSO-UHFFFAOYSA-N m-propyltoluene Chemical compound CCCC1=CC=CC(C)=C1 QUEBYVKXYIKVSO-UHFFFAOYSA-N 0.000 description 6
- 239000012855 volatile organic compound Substances 0.000 description 6
- 239000011521 glass Substances 0.000 description 4
- UOHMMEJUHBCKEE-UHFFFAOYSA-N prehnitene Chemical compound CC1=CC=C(C)C(C)=C1C UOHMMEJUHBCKEE-UHFFFAOYSA-N 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- -1 VOCs Natural products 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000004224 protection Effects 0.000 description 3
- 229910021642 ultra pure water Inorganic materials 0.000 description 3
- 239000012498 ultrapure water Substances 0.000 description 3
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000189579 Thripidae Species 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 description 2
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 2
- 239000012159 carrier gas Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
- 235000003642 hunger Nutrition 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000037351 starvation Effects 0.000 description 2
- 150000003505 terpenes Chemical class 0.000 description 2
- 235000007586 terpenes Nutrition 0.000 description 2
- SPSVMMKHVNWAFN-UHFFFAOYSA-N 1,3-xylene Chemical compound CC1=CC=CC(C)=C1.CC1=CC=CC(C)=C1 SPSVMMKHVNWAFN-UHFFFAOYSA-N 0.000 description 1
- ODCUUYDFYXTJIH-UHFFFAOYSA-N C(C)C1=C(C=C(C=C1)C)C.C(C)C1=C(C=C(C=C1)C)C Chemical compound C(C)C1=C(C=C(C=C1)C)C.C(C)C1=C(C=C(C=C1)C)C ODCUUYDFYXTJIH-UHFFFAOYSA-N 0.000 description 1
- 240000004160 Capsicum annuum Species 0.000 description 1
- 235000002567 Capsicum annuum Nutrition 0.000 description 1
- 239000004803 Di-2ethylhexylphthalate Substances 0.000 description 1
- MQIUGAXCHLFZKX-UHFFFAOYSA-N Di-n-octyl phthalate Natural products CCCCCCCCOC(=O)C1=CC=CC=C1C(=O)OCCCCCCCC MQIUGAXCHLFZKX-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001256156 Frankliniella minuta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000337007 Oceania Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 235000010354 butylated hydroxytoluene Nutrition 0.000 description 1
- 239000001511 capsicum annuum Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- XTCQUBCCCSJAKJ-UHFFFAOYSA-N ethylbenzene Chemical compound CCC1=CC=CC=C1.CCC1=CC=CC=C1 XTCQUBCCCSJAKJ-UHFFFAOYSA-N 0.000 description 1
- 238000000556 factor analysis Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000008186 parthenogenesis Effects 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- YYGNTYWPHWGJRM-AAJYLUCBSA-N squalene Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CC\C=C(/C)CC\C=C(/C)CCC=C(C)C YYGNTYWPHWGJRM-AAJYLUCBSA-N 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of a kidney bean volatile substance in attracting an invading pest Cisii-Ardisia Arctii, wherein the kidney bean volatile substance is di (2-ethylhexyl) phthalate, trans-squalene or ethylbenzene. Use of di (2-ethylhexyl) phthalate or trans-squalene or ethylbenzene for attracting the invading pest Cithistle. An attractant for attracting the invading pest, called thrips occidentalis, contains di (2-ethylhexyl) phthalate or trans-squalene or ethylbenzene as its active component. The three substances which can be used as the source of the attractant for the invading pest Frankliniella occidentalis can be bought in the market, can be extracted from the volatile substances of host plant Phaseolus vulgaris beans of Frankliniella occidentalis, can be used for producing the Frankliniella occidentalis attractant or can be used for attracting Frankliniella occidentalis by directly planting Phaseolus vulgaris beans with a certain area, has the characteristics of no pollution, high efficiency and stable performance, is suitable for the production of organic vegetables and pollution-free vegetables, and is convenient to transport.
Description
Technical Field
The invention belongs to the technical field of green prevention and control of pests, and particularly relates to application of an existing substance in attracting an invading pest Frankliniella occidentalis.
Background
Frankliniella occidentalis (Frankliniella occidentalis) also known as alfalfa thrips, belonging to the order Thysanoptera (Thysanoptera), thrips (Thripidae). The insect was native to north america, was first discovered in 1955 in hawaii, and was once the most common thrips in california in the united states. Because the individual is tiny and is good at hiding, the flower tree is easy to spread along with the cross-border circulation of flowers and trees between international places and regions. From the 80 s of the 20 th century, western thrips has gradually expanded outward, successively attacking canada, uk, australia, etc. Until now, frankliniella occidentalis has spread over nearly 70 countries and regions, such as america, europe, asia, africa, and oceania, and has become a quarantine pest worldwide. As early as 1996, frankliniella occidentalis was classified as a potentially dangerous pest for entry plant quarantine by the Ministry of agriculture in China. In summer of 2003, the pest is collected from the flowers of capsicum annuum in suburbs of Beijing, which is the research site of animals of Chinese academy of sciences, and is made into a specimen, and the pest is identified as a novel invasive pest, namely the frankliniella occidentalis, which is the first report about occurrence of the frankliniella occidentalis in China. Under the stable environmental condition in a greenhouse, the western flower thrips can continuously generate 12-15 generations in one year, adults have the habit of pupation by burying in soil, the female reproduction mode is amphoteric reproduction and parthenogenesis, the female flower thrips can develop at 15-35 ℃, the eggs lay at 27 ℃ most, the egg laying amount of single female is more than 200, the development speed is high, and only half a month is needed from eggs to adults. Under the field condition, the average egg period of the Frankliniella occidentalis is 5-15 d, the development duration of nymphs is 9-12 d, the prepupa period lasts for 1-3 d, the pupa period lasts for 3-10 d, and the service life of adults is usually 20-30 d. The reproductive capacity of the frankliniella occidentalis is very strong, and the general control method is difficult to achieve effective control effect in the field.
Volatile Organic Compounds (VOCs) released by plants are important links for mediating chemical communication within and between plant species, between plants and insects, and between plants and natural enemies of pests, including regulation of pollination, seed germination, protection against phytophagous insects, parasites, viruses, and the like. Plant volatiles have been one of the hot research spots in the field of plant protection and in chemical ecology for the last three decades. In recent years, researchers at home and abroad have made a lot of progress in research on biosynthesis, metabolism, and functions of secondary metabolites such as VOCs, and interaction with insects. Plant volatiles are odorous substances emitted from the surface of the aerial parts of plants, such as leaves, flowers and shoots, and released by self-biosynthesis during different development stages of the plants, and are generally a mixture of various micro-concentrations of volatile secondary substances, rather than a single substance. The relative molecular mass is generally between 100-200, and the main components are alkanes, aromatic hydrocarbons, alcohols, ketones, aldehydes, esters, phenols, organic acids, terpenes and the like, wherein the VOCs related to the regulation of insect behaviors are mainly phenols, terpenes, alkaloids and green leaf gases.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provide a plant and a specific substance which can be used as an attractant source for invading pest frankliniella occidentalis.
The invention aims to solve the problems by the following technical scheme:
the application of the kidney bean volatile matter in attracting the invading pest of the artichoke is characterized in that: the volatile matter of the kidney beans is di (2-ethylhexyl) phthalate, or trans-squalene, or ethylbenzene.
Use of di (2-ethylhexyl) phthalate or trans-squalene or ethylbenzene for attracting the invading pest Cithistle.
The di (2-ethylhexyl) phthalate or trans-squalene or ethylbenzene can attract the emerging adult Frankliniella occidentalis.
An attractant for attracting the invading pest, called thrips occidentalis, contains di (2-ethylhexyl) phthalate or trans-squalene or ethylbenzene as its active component.
The attractant is a plant source attractant.
Compared with the prior art, the invention has the following advantages:
the three substances which can be used as the source of the attractant for the invading pest Frankliniella occidentalis can be bought in the market, and can be extracted from the volatile matters of the host plant Phaseolus vulgaris, namely the plant source active substances extracted from the volatile matters of the Phaseolus vulgaris: di (2-ethylhexyl) phthalate, trans-squalene and ethylbenzene can be used for producing the Frankliniella occidentalis attractant or used for attracting Frankliniella occidentalis by directly planting kidney beans with a certain area.
When the volatile matter of the kidney beans provided by the invention is used for attracting frankliniella occidentalis, the volatile matter of the kidney beans has the characteristics of no pollution, high efficiency and stable performance, is suitable for the current production of organic vegetables and pollution-free vegetables, and is convenient to transport.
Drawings
FIG. 1 is a schematic structural view of a plant volatile dynamic headspace collection device employed in the present invention;
FIG. 2 is a graph showing the behavioral response of Frankliniella occidentalis to six low-concentration plant volatiles from the host plant Phaseolus vulgaris;
FIG. 3 is a graph showing the behavioral response of Frankliniella occidentalis to plant volatiles from the six medium concentrations of the host plant Phaseolus vulgaris;
FIG. 4 is a graph showing the behavioral response of Frankliniella occidentalis to six high concentration plant volatiles from the host plant Phaseolus vulgaris.
Wherein: 1-air pump; 2-a flow meter; 3, washing the gas cylinder with ultrapure water; 4-active carbon drying tower; 5-an adsorption container; 6, an adsorption tube.
Detailed Description
The invention is further described with reference to the following figures and examples.
Collecting kidney bean volatile substances by adopting plant volatile substance dynamic headspace collecting device
The plant volatile dynamic headspace collecting device is shown in figure 1. The device comprises an air pump 1, a flowmeter 2, an ultrapure water gas washing bottle 3, an activated carbon drying tower 4, an adsorption container (glass cylinder) 5 and an adsorption pipe 6, wherein the glass cylinder 5 consists of an upper part and a lower part, the inner diameter is 20cm, the height is 60cm, the bottom of the lower half part of the glass cylinder 5 is connected with the adsorption pipe 6, and the adsorption pipe 6 is placed into the lower half part with the inner diameter of 0.1cm and is divided into an air inlet and an air outlet.
5 host plant beans were selected and placed in a 2000ml beaker, ultrapure water was added until the underground part of the plant was submerged, and then the beaker was placed in a glass jar. And (3) filling an adsorption tube 6 in a sterilized 30-min ultra-clean workbench, connecting a plant volatile dynamic headspace collecting device after filling, connecting a power supply, adjusting the flow rate to be 300ml/min, and performing adsorption for 10-12 h.
And leaching the collected adsorption tube 6 by using normal hexane, wherein the gas outlet end of the adsorption tube 6 is connected with a sample bottle with a transparent threaded opening. And after the n-hexane completely flows into the sample bottle, sucking 2-3 ml of n-hexane again, pumping into the adsorption tube 6, standing for 10min, and blowing the n-hexane left in the adsorption tube 6 into the sample bottle by using high-purity nitrogen.
Connecting high-purity nitrogen to a Pasteur tube to slowly blow nitrogen onto the sample, and concentrating to 400-500 ml.
Second, separation and identification of volatile substances
The instrument comprises the following steps: gas chromatography-mass spectrometer (GC-MS, 320-MS, Brukton Mass Spectrometry, USA).
Chromatographic conditions are as follows: HP-5 Agilent capillary column (30cm × 0.25mm × 0.25mm), injection port temperature: he is used as carrier gas at 250 ℃, the flow rate of the carrier gas is 1.2ml/min, and split-flow sample injection is not carried out.
Mass spectrum conditions: the ion source is EI70eV, 250 ℃, the voltage is 350V, the interface temperature is 280 ℃, and the scanning voltage range is 50-550 m/Z. Temperature rising procedure: the initial temperature is 40 ℃, the temperature is increased to 80 ℃ at 3 ℃/min, and the temperature is increased to 260 ℃ at 5 ℃/min and is kept for 5 min. Mu.l of sample was injected each time.
The GC-MS result shows that 30 volatile matters separated and identified from the kidney beans belong to ten plant volatile matters such as aromatic hydrocarbon, ether, alkane, cyclane, alcohol, olefin, aromatic derivative, phenol, ketone, ester and the like, and the classification table is shown in Table 1.
Control and multiplication of CO by undamaged plants (CK)2The compositional percentage change of 30 Volatile Organic Compounds (VOCs) after mechanical injury (MD) and pest Treatment (TI) of kidney beans (p. vulgaris) at concentration was subjected to CO2The concentration levels, wound type and their interaction two-factor analysis of variance (F/P values) are shown in Table 2.
As is clear from Table 2, the contents of 9 compounds such as ethylbenzene, m-xylene, 1-methyl-3-propylbenzene, 1, 3-dimethyl-4-ethylbenzene, m-isopropyltoluene, 1,2,3, 4-tetramethylbenzene, trans-squalene, 2, 6-di-t-butyl-p-cresol di (2-ethylhexyl) phthalate and the like are influenced by environmental CO2Concentration level, frankliniella occidentalis harmfulness and significant influence of interaction of the concentration level and the frankliniella occidentalis harmfulness; in view of the same number of carbon atoms, similar structure and chemical properties, of 1-methyl-3-propylbenzene, 1, 3-dimethyl-4-ethylbenzene, m-isopropyltoluene and 1,2,3, 4-tetramethylbenzene, 1, 3-dimethyl-4-ethylbenzene is subjected to CO2The concentration level is more influenced, so 1,3-dimethyl-4-ethyl benzene is selected to represent four kinds of plants such as 1-methyl-3-propyl benzene, 1,3-dimethyl-4-ethyl benzene, m-isopropyl toluene and 1,2,3, 4-tetramethyl benzeneThe material volatile is a research object; namely, six kinds of volatile substances such as ethylbenzene, 1, 3-dimethylbenzene, 1, 3-dimethyl-4-ethylbenzene, trans-squalene, 2, 6-di-t-butyl-p-cresol, and di (2-ethylhexyl) phthalate were selected as the subjects to be studied.
Third, behavior determination of Frankliniella occidentalis
(one) test insects
The method comprises the following steps of breeding the frankliniella occidentalis in a laboratory breeding population in an intelligent artificial climate box (RXZ-500C; Ningbo southeast Instrument factory; Ningbo, China), and breeding the leguminous kidney bean pods in an octagonal pot containing the frankliniella occidentalis for test use. The kidney bean pods are replaced every two days and the octagonal pot is rotated to ensure uniform illumination. Setting parameters of the incubator: the temperature is 26 +/-1 ℃, the relative humidity is 70%, and the photoperiod L: D is 16h:8 h.
(II) test volatiles
Standard products such as ethylbenzene (Ethyl benzene), 1, 3-dimethylbenzene (1,3-Dimethyl benzene), 1, 3-Dimethyl-4-ethylbenzene (1,3-Dimethyl-4-Ethyl benzene), trans-Squalene ((E, E, E) -Squalene), 2, 6-di-tert-butyl-p-cresol (2, 6-Dimethyl-butyl-4-methyl phenol) and di-2-ethylhexyl phthalate (Dioctyl phthalate) are all available from Beijing chromatography science and technology Limited, and the purity of 1, 3-Dimethyl-4-ethylbenzene is 95% or more and the purity of other compounds is 99.5% or more.
(III) preparation of lure and hollow core
N-hexane (analytically pure, shoudebio-technology limited, jiangsu Nanjing) is used as a solvent for diluting a substance to be detected, 0.1ml of a solution to be detected (e.g., 40.71 μ l of m-xylene and 57.29 μ l of N-hexane, which form a solution to be detected with a concentration of 40.71%) and N-hexane are respectively dropped on a filter paper (r ═ 1cm) with the same area to prepare a lure core and a blank core of the substance to be detected, and the lure core and the blank core are placed into pear-shaped bottles at two ends of a four-wall olfactometer (PSM 4-150; Nanjing Pmpson instruments and instruments limited, Jiangsu Nanjing) for behavior determination. Experimental concentrations of the kidney bean specific volatile components are shown in Table 3.
TABLE 3 test 6 plant volatiles from the host plant Phaseolus vulgaris and their volume to volume concentration settings (V/V%)
(IV) Experimental methods
The four-wall olfactometer (PSM 4-150; Nanjing Pusen instruments and equipments Limited, Jiangsu Nanjing) is composed of a four-arm main board, a pear-shaped bottle, a drying tower, a gas washing bottle, a flow meter and an air pump, wherein the diagonal line of the main board of the four-wall olfactometer is 35cm in length, 29cm in width, 4.3cm in height and the included angle of two arms is 90 degrees. During the test, a 8W fluorescent lamp is placed right above the four-wall olfactometer. The reliability of the instrument was tested by placing 30 frankliniella occidentalis into a four-wall olfactometer without the introduction of the odorant compound, and the number of frankliniella occidentalis entering 4 regions in 3 repeated tests was not significantly different and was therefore used for the test. The air pump is opened to be connected with the power supply, the joint of the device is adjusted, whether air leaks is checked, and when bubbles emerge from all the four gas washing bottles, the air tightness of the device is proved to be good; switching on a power supply, adjusting the flow rate of the flow meter to ensure that the flow rates of the gas passing through the four arms are the same and are all 100ml/min, and turning on a fluorescent lamp arranged right above the four-arm mainboard to ensure that the four-wall olfactometer is uniformly illuminated; placing the flavor compound in a flavor bottle, and then ventilating for 5 min; selecting 30 heads of emerging Frankliniella occidentalis adults, performing starvation treatment for 8h, and then connecting the imagoes to an air suction port of a four-wall olfactometer, wherein the Frankliniella occidentalis enters an arm tail (a dotted line area with the tail end of the arm as a circle center and a radius of 5 cm) of one arm or enters a pear-shaped bottle through the arm tail within 20min, and then determining that the Frankliniella occidentalis has tropism to the odor of the arm, and if the Frankliniella occidentalis does not enter the four areas within 20min, determining that the Frankliniella occidentalis is not selected. In the test, 30 starvation-treated frankliniella occidentalis are tested each time, and each treatment is repeated for 3 times; in order to avoid the influence of illumination, airflow, the position effect of each arm of the four-wall olfactometer and the like on the selection of thrips, the position of the same processing flavor source is changed after each processing test.
(V) results of the experiment
Studies of the behavioral response of Frankliniella occidentalis to six plant volatiles (ethylbenzene, meta-xylene, 1, 3-dimethyl-4-ethylbenzene, trans-squalene, 2, 6-di-tert-butyl-P-cresol, di (2-ethylhexyl) phthalate) from the host plant phaseolus vulgaris by means of a four-walled olfactometer are shown in FIGS. 2-4, which results show, by means of the independent variance t test, a significant difference between treatment and control (P < 0.05), indicating that low concentrations of di (2-ethylhexyl) phthalate, medium concentrations of trans-squalene, and high concentrations of ethylbenzene have a significant attractive effect on Frankliniella occidentalis.
It should be noted that the above examples and test examples are only for further illustration and understanding of the technical solutions of the present invention, and are not to be construed as further limitations of the technical solutions of the present invention, and the invention which does not highlight essential features and significant advances made by those skilled in the art still belongs to the protection scope of the present invention. The technology not related to the invention can be realized by the prior art.
Claims (6)
1. The application of the kidney bean volatile matter in attracting the invading pest of the artichoke is characterized in that: the volatile matter of the kidney beans is di (2-ethylhexyl) phthalate, or trans-squalene, or ethylbenzene.
2. The use of kidney bean volatiles to attract the invading pest artichoke (thistle) as claimed in claim 1 wherein: the di (2-ethylhexyl) phthalate or trans-squalene or ethylbenzene can attract the emerging adult Frankliniella occidentalis.
3. Use of di (2-ethylhexyl) phthalate or trans-squalene or ethylbenzene for attracting the invading pest Cithistle.
4. Use according to claim 3, characterized in that: the di (2-ethylhexyl) phthalate or trans-squalene or ethylbenzene can attract the emerging adult Frankliniella occidentalis.
5. An attractant capable of attracting an invading pest, namely frankliniella occidentalis, is characterized in that: the effective component of the attractant is di (2-ethylhexyl) phthalate or trans-squalene or ethylbenzene.
6. An attractant capable of attracting the invading pest thrips occidentalis according to claim 5, wherein: the attractant is a plant source attractant.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201642741A (en) * | 2015-04-01 | 2016-12-16 | 協友股份有限公司 | Combination of harmful arthropod-attracting compound and harmful arthropod-repelling compound |
| CN109730065A (en) * | 2018-07-23 | 2019-05-10 | 黑龙江省植检植保站 | A kind of lure composition of Frankliniella occidentalis |
| CN111296453A (en) * | 2020-03-30 | 2020-06-19 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Vegetable pest thrips occidentalis attractant and attracting device thereof |
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|---|---|---|---|---|
| TW201642741A (en) * | 2015-04-01 | 2016-12-16 | 協友股份有限公司 | Combination of harmful arthropod-attracting compound and harmful arthropod-repelling compound |
| CN109730065A (en) * | 2018-07-23 | 2019-05-10 | 黑龙江省植检植保站 | A kind of lure composition of Frankliniella occidentalis |
| CN111296453A (en) * | 2020-03-30 | 2020-06-19 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Vegetable pest thrips occidentalis attractant and attracting device thereof |
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