CN114885942B - Attractant for bemisia tabaci and application thereof - Google Patents
Attractant for bemisia tabaci and application thereof Download PDFInfo
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- CN114885942B CN114885942B CN202210625174.1A CN202210625174A CN114885942B CN 114885942 B CN114885942 B CN 114885942B CN 202210625174 A CN202210625174 A CN 202210625174A CN 114885942 B CN114885942 B CN 114885942B
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- bemisia tabaci
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention provides a bemisia tabaci attractant and application thereof, belongs to the technical field of bemisia tabaci biological control, and particularly relates to application of p-ethyl acetophenone in bemisia tabaci prevention and control, wherein the effective component of the bemisia tabaci attractant comprises p-ethyl acetophenone; the invention discovers that the compound p-ethyl acetophenone has a certain attraction effect on bemisia tabaci for the first time, and the invention utilizes the p-ethyl acetophenone to prevent and control the bemisia tabaci without pollution, accords with the green prevention and control technology, and can reduce the use of chemical pesticides in fields.
Description
Technical Field
The invention belongs to the technical field of biological control of bemisia tabaci, and particularly relates to an attractant for bemisia tabaci and application thereof.
Background
Bemisia tabaci (Gennadius), commonly known as Bemisia tabaci (cotton white) or Bemisia tabaci (sweetpotato whitefly), and the like, belong to the family of Hemiptera (Hemiptera) whitefly (Aleyrodidae), and mainly live in tropical and subtropical areas. The insect has strong fertility, strong adaptability and easy generation of drug resistance, and part of hidden seeds have strong invasiveness and outbreak, and are called super pests. Bemisia tabaci hosts are broad, up to 1000 species, and are considered to be species complexes (species complexes) containing at least 44 cryptospecies. It is counted that bemisia tabaci can spread at least about 320 plant viruses contained in 5 families, resulting in a huge economic loss.
Tomato chlorosis virus (Tomato chlorosis virus, toCV) is belonging to the genus Chaetoceros (Crinivirus) of the family Leptoviridae (Closterovi ridae). After the tomato is infected, the plants are green in pulse, the leaves are withered and yellow, and when serious, the leaves are necrotic and dry and fall off, the fruits are malformed, the quality is seriously reduced, and the commercial value is lost. The bemisia tabaci is found to be the main transmitter of ToCV viruses in China and other countries.
At present, most people adopt chemical pesticide spraying as a main means, but a large amount of chemical pesticide spraying processes not only can cause insect to generate drug resistance, but also can pollute the environment to a certain extent and threaten human health to cause 3R problems and the like, and the use of plant source compounds as biological control medicaments is always an important green biological control means in the plant protection industry, and has wide application prospect due to the characteristics of basically no residue, easy degradation, environmental friendliness and the like. For example, rotenone, nicotine, pyrethrin and other medicines extracted from rotenone, tobacco, pyrethrum and the like have good insecticidal effects; azadirachtin, as extracted from neem, has also been shown to have a potent insecticidal effect.
The bemisia tabaci has strong fertility and adaptability, and especially, the propagation of plant viruses is extremely easy to cause huge economic loss. Therefore, the invention is necessary for attracting or repelling bemisia tabaci, meets the aim of sustainable control of pests, is beneficial to green control of bemisia tabaci, protects natural enemies and controls the spread of plant viruses such as ToCV. There are few reports in the prior art on bemisia tabaci attractants.
Chinese patent document CN102630671a (application number: 201210083281.2) discloses a tussah decorating ventral parasitic fly attractant and application thereof, the attractant comprises the following three components: beta-cis ocimene, alpha-farnesene and p-ethyl acetophenone, the tussah decorating abdominal drosophila is attracted. The attracted pests are tussah ventral parasitic flies belonging to diptera, the parasitic fly family, blepharia genus; the insect pest attracted in the invention is bemisia tabaci belonging to the order of hemiptera, the family of whitefly and the genus of whitefly.
Chinese patent document CN103960276 (application No. 201410178932.5) discloses a black guangdong step a attractant and lure, the attractant is made of beta-trans ocimene, alpha-farnesene, linalool, p-ethylacetophenone, oanthraquinone, cis-3-hexen-acetate, cis-3-hexen-1-ol, n-tetradecane, n-pentadecane and n-heneicosane. The composition of the attractant disclosed in the patent document is complex, and the attracted pests are the Guangdong Jia belonging to coleoptera and the Paracolatae; the insect pest attracted in the invention is bemisia tabaci belonging to the order of hemiptera, the family of whitefly and the genus of whitefly.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an attractant for bemisia tabaci and application thereof
The invention provides a plant source volatile matter for attracting bemisia tabaci to gather, which is used for improving the effect of preventing and controlling bemisia tabaci.
The invention finds that bemisia tabaci tends to select healthy tomato plants after feeding tomato plants infected by ToCV virus, and then the volatile components and the content of the virus-infected tomatoes and uninfected healthy tomatoes are analyzed by GC-MS gas chromatography-mass spectrometry technology. As a result, it was found that p-ethylacetophenone, a compound, could be detected in the aerosol fraction of healthy tomatoes, but not in tomato plants infected with ToCV virus.
The invention discovers that the compound p-ethyl acetophenone has a certain attraction effect on bemisia tabaci and also has an attraction effect on bemisia tabaci carrying toxic ToCV. Therefore, the compound p-ethylacetophenone is used as a research object, and the Y-type olfactometer is used for testing the effect of an olfactory system in the selection of bemisia tabaci hosts; meanwhile, the influence of the compound treatment tomato on the selection behavior of bemisia tabaci hosts is explored by simulating the field drug application situation.
The technical scheme of the invention is as follows:
the application of the p-ethylacetophenone in the prevention and control of bemisia tabaci.
A bemisia tabaci attractant, the components comprising p-ethyl acetophenone.
According to a preferred embodiment of the invention, the attractant further comprises paraffin.
Further preferably, the mass volume ratio of the p-ethylacetophenone to the paraffin wax is (150-220): 1mg/L.
More preferably, the mass volume ratio of the p-ethylacetophenone to the paraffin is 200:1mg/L.
According to a preferred embodiment of the invention, the attractant further comprises methanol.
Further preferably, the molar volume ratio of p-ethylacetophenone to methanol is (5-11): 1mmoL/L.
More preferably, the molar volume ratio of p-ethylacetophenone to methanol is 10:1mmoL/L.
The application of the attractant in the prevention and control of bemisia tabaci.
According to a preferred embodiment of the invention, the attractant is used in combination with tobacco in the control of bemisia tabaci.
According to a preferred embodiment of the invention, the attractant is used for controlling Bemisia tabaci carrying ToCV virus.
The application of the attractant in preventing and controlling plant ToCV virus diseases caused by whiteflies carrying ToCV virus cigarettes.
Preferably, according to the invention, the plant is tomato, tobacco.
Advantageous effects
1. The invention discovers that the compound p-ethyl acetophenone has a certain attraction effect on bemisia tabaci for the first time, and the invention utilizes the p-ethyl acetophenone to prevent and control the bemisia tabaci without pollution, accords with the green prevention and control technology, and can reduce the use of chemical pesticides in fields.
2. The attractant provided by the invention can effectively solve the problem of pollution caused by bemisia tabaci prevention and control, and has wide application prospect due to the characteristics of basically no residue, environmental friendliness and the like of the plant.
3. The attractant provided by the invention has better attraction effect on the bemisia tabaci carrying the ToCV virus, and is beneficial to preventing and controlling plant ToCV virus diseases caused by the bemisia tabaci carrying the ToCV virus.
4. The attractant provided by the invention is combined with tobacco, and has better attraction effect on bemisia tabaci.
Drawings
FIG. 1 is a graph showing the results of the olfactory behavior of toxic ToCV bemisia tabaci against p-ethyl acetophenone.
Fig. 2 is a schematic diagram of a device for testing the selective behavior of bemisia tabaci on p-ethyl acetophenone.
FIG. 3 is a graph showing the results of the selection of healthy bemisia tabaci for p-ethyl acetophenone.
FIG. 4 is a graph showing the results of the selection of toxic ToCV bemisia tabaci versus p-ethyl acetophenone.
Fig. 5 is a schematic diagram of a device for testing the selective behavior of bemisia tabaci on p-ethyl acetophenone.
Fig. 6 is a graph showing the results of the selection of healthy bemisia tabaci versus p-ethyl acetophenone.
Detailed Description
The technical scheme of the present invention is further described in conjunction with the following examples, but the scope of the present invention is not limited thereto.
What is not described in detail in the examples is known in the art.
Example 1
The effect of the olfactory system in bemisia tabaci host selection behavior is tested by using a Y-type olfactory instrument, and the specific steps are as follows:
step 1, olfactory behavior determination adopts a Y-shaped tube olfactometer, and the whole connection mode is as follows: air pump, active carbon, molecular sieve, allochroic silica gel, gas washing bottle, flowmeter, Y-shaped pipe, gas connecting part comprising soft sterilized silica gel pipe, and 15W-6500K energy-saving lamp set up above the whole. When in use, the whole Y-shaped pipe olfactometer is wiped by absolute alcohol to remove dust and other messy organic compounds and grease, and the system is balanced by ventilation for 30min after airing.
And 2, setting a p-ethylacetophenone paraffin solution with the concentration of 200 mg/L. 100. Mu.L of p-ethylacetophenone solution was pipetted into a filter paper strip and placed into a gas bottle with liquid paraffin as the control. Setting the gas flow rate to be 200mL/min, testing the olfactory selection behavior of each head of bemisia tabaci in a Y-shaped pipe, placing a single head of bemisia tabaci in the inlet of the Y-shaped pipe for about 5cm, and then observing the behavioral response of the bemisia tabaci in 3 min. 60 head of the toCV bemisia tabaci carrying toxin is tested in total, and every 20 head of bemisia tabaci is replaced by a gas washing bottle, and the gas washing bottle is wiped by absolute ethyl alcohol and dried before being used.
The experimental results are shown in fig. 1: compared with liquid paraffin, the bemisia tabaci obviously tends to be p-ethylacetophenone paraffin solution, and the p-ethylacetophenone has obvious attraction effect on the bemisia tabaci on sense of smell.
Example 2
The medicine p-ethylacetophenone is prepared into 10mM concentration methanol diluent, and the methanol diluent is sprayed on the surfaces of healthy tomato plant leaves by using a small handheld atomizer under the condition of spraying 3, and the same growing tomatoes are additionally selected to use the methanol to spray the same amount as a control. The medicine treated tomato plant and the methanol control tomato plant are placed in an insect-proof net at opposite angles, the distance between the opposite angles is about 50cm, and the tomato plant and the methanol control tomato plant stand for 10min. The healthy pairs of bemisia tabaci 50 were drawn in pairs for a total of 100 heads in the sample tubes, with the sample tube ports placed up to a central position equidistant from the tomatoes of the treatment and control groups for release (see fig. 2), and the number of bemisia tabaci falling on the tomato leaves was carefully counted at regular intervals. The dead individuals were not counted during the duration of the test, and finally the ratio was calculated for each test group, and the experimental results are shown in fig. 3.
The calculation formula of the ratio is: for compound selectivity = number of selected compound treated plant bemisia tabaci/(number of selected compound treated plant bemisia tabaci + number of selected methanol treated plant bemisia tabaci) ×100%.
The selection results of bemisia tabaci are shown in figure 3, the difference of the selection results is not obvious in the time periods of 6h and 72h, and the difference is obvious (P is less than or equal to 0.05) in the rest time periods, wherein healthy bemisia tabaci is a tomato plant tending to spray P-ethyl acetophenone methanol solution. The tomato spraying rate of the bemisia tabaci selection medicine tends to be stable at 55%.
Example 3
The medicine p-ethylacetophenone is prepared into 10mM concentration methanol diluent, and the methanol diluent is sprayed on the surfaces of healthy tomato plant leaves by using a small atomizer under the condition of spraying 3, and the same growing tomatoes are additionally selected to use methanol spraying with equal amount as a control. The medicine treated tomato plant and the methanol control tomato plant are placed in an insect-proof net at opposite angles, the distance between the opposite angles is about 50cm, and the tomato plant and the methanol control tomato plant stand for 10min. The pairs of toxic ToCV bemisia tabaci 50 in the sucking population are combined into 100 sample tubes, the mouths of the pairs are upwards placed in the center positions equidistant to tomatoes in the treatment group and the control group, the pairs are released, and the number of bemisia tabaci falling on tomato leaves is carefully counted at fixed intervals. The dead individuals were not counted during the duration of the test, and finally the ratio was calculated for each test group, and the experimental results are shown in fig. 4.
Bemisia tabaci selection results as shown in FIG. 4, the selection results were not significantly different over the 24h, 48h, 72h and 96h time periods. In the rest period, the number of tomato plants sprayed by the toxic ToCV bemisia tabaci selected P-ethyl acetophenone methanol solution is more, and the difference is remarkable (P is less than or equal to 0.05). The tomato spraying rate of the bemisia tabaci selection medicine tends to be stable at 60%.
Example 4
P-ethylacetophenone was prepared as a 10mM concentration methanol dilution and sprayed onto leaf surfaces of healthy tobacco plants using a small atomizer under 3 spray conditions, and the same growing tobacco was additionally selected as a control using methanol spray in equal amounts. The medicine treated tobacco strain and the methanol control tobacco strain are placed in an insect-proof net at opposite angles, the distance between the opposite angles is about 50cm, and the tobacco strain is kept stand for 10min. The healthy whiteflies 50 in the population were drawn in pairs in a total of 100 heads in the sample tube, placed mouth up in a central position equidistant from the treatment and control group tobacco, released (see fig. 5), and carefully counted at regular intervals for the number of whiteflies falling on the tobacco leaves. The dead individuals were not counted during the duration of the test, and finally the ratio was calculated for each test group, and the experimental results are shown in fig. 6.
Bemisia tabaci selection results as in FIG. 6, the difference in selection results was insignificant only over a 3h period. In the rest period, the tobacco plants sprayed by the Bemisia tabaci selected from the P-ethyl acetophenone methanol solution are more in number, so that the difference is remarkable (P is less than or equal to 0.05). In the latter half of the overall selection time, the bemisia tabaci selection drug sprayed tobacco ratio tended to stabilize at 62%.
Example 5
The difference from example 4 is that the drug p-ethylacetophenone was prepared as a methanol dilution with a concentration of 14mM, all other conditions being identical.
Bemisia tabaci selection results: the tobacco spraying ratio of the Bemisia tabaci to the ethyl acetophenone tends to be stable to be 51%, and compared with the methanol diluent prepared by preparing the medicine of the ethyl acetophenone into the concentration of 10mM in the embodiment 4, the tobacco spraying ratio has a lower effect, but has a certain effect.
From the above results, it can be seen that the p-ethyl acetophenone has attraction capability to bemisia tabaci and can be used as a bemisia tabaci attractant for biological control; when the p-ethyl acetophenone is used for attracting the bemisia tabaci, the attraction capacity can be reduced due to the fact that the use concentration is too high, so that the attraction effect of the p-ethyl acetophenone on the bemisia tabaci is better under the proper concentration. The attractant provided by the invention has better attraction effect on the bemisia tabaci carrying the ToCV virus, and is beneficial to preventing and controlling plant ToCV virus diseases caused by the bemisia tabaci carrying the ToCV virus. The attractant provided by the invention is combined with tobacco, and has better attraction effect on bemisia tabaci.
Claims (7)
1. The application of the p-ethyl acetophenone as an attractant in the prevention and control of bemisia tabaci, wherein the attractant also contains paraffin or methanol; the mass volume ratio of the p-ethylacetophenone to the paraffin is (150-220): 1 mg/L; the mol volume ratio of the p-ethylacetophenone to the methanol is (5-11): 1mmoL/L.
2. Use according to claim 1, characterized in that the mass-to-volume ratio of p-ethylacetophenone to paraffin is 200:1mg/L.
3. Use according to claim 1, characterized in that the molar volume ratio of p-ethylacetophenone to methanol is 10:1mmoL/L.
4. Use according to any one of claims 1 to 3, wherein the attractant is used in combination with tobacco in the control of bemisia tabaci.
5. Use according to any one of claims 1 to 3, wherein the attractant is used for controlling bemisia tabaci carrying a ToCV virus.
6. Use of an attractant in accordance with any one of claims 1 to 3 for controlling a disease of a plant ToCV virus caused by a Bemisia tabaci carrying the ToCV virus.
7. The use according to claim 6, wherein the plant is tomato, tobacco.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210625174.1A CN114885942B (en) | 2022-06-02 | 2022-06-02 | Attractant for bemisia tabaci and application thereof |
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| CN202210625174.1A CN114885942B (en) | 2022-06-02 | 2022-06-02 | Attractant for bemisia tabaci and application thereof |
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| CN114885942B true CN114885942B (en) | 2023-04-28 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103960276B (en) * | 2014-04-30 | 2015-10-28 | 辽宁省蚕业科学研究所 | A kind of black ground beetle attractant and lure core |
| CN107047554A (en) * | 2017-05-12 | 2017-08-18 | 中国科学院植物研究所 | With natural plant essential oils attractant that function is trapped for Bemisia tabaci and preparation method thereof |
| CN109730083A (en) * | 2019-02-02 | 2019-05-10 | 湖南省植物保护研究所 | Bemisia tabaci approach behavior adjusting control agent, the application of insecticide and insecticide, the application of desinsection plate and desinsection plate |
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