CN109730083A - Bemisia tabaci approach behavior adjusting control agent, the application of insecticide and insecticide, the application of desinsection plate and desinsection plate - Google Patents
Bemisia tabaci approach behavior adjusting control agent, the application of insecticide and insecticide, the application of desinsection plate and desinsection plate Download PDFInfo
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- CN109730083A CN109730083A CN201910107424.0A CN201910107424A CN109730083A CN 109730083 A CN109730083 A CN 109730083A CN 201910107424 A CN201910107424 A CN 201910107424A CN 109730083 A CN109730083 A CN 109730083A
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Abstract
The present invention provides the applications of a kind of Bemisia tabaci approach behavior adjusting control agent, insecticide and insecticide, the application of desinsection plate and desinsection plate.Bemisia tabaci approach behavior adjusting control agent is neophytadiene, and neophytadiene has the function of luring Bemisia tabaci, can design prevention and treatment of the Bemisia tabaci approach behavior adjusting control agent for Bemisia tabaci.The present invention also provides a kind of insecticides and desinsection plate, neophytadiene is added in pesticide or yellow plate, the addition of neophytadiene can significantly improve insecticide or yellow plate to the killing effect of Bemisia tabaci, it can be applied to the prevention and treatment to Bemisia tabaci, have the advantages that safety non-pollution, dosage are small, active strong.
Description
Technical field
The present invention relates to pest control technical field more particularly to Bemisia tabaci approach behavior adjusting control agent, insecticide and kill
The application of worm agent, the application of desinsection plate and desinsection plate.
Background technique
Bemisia tabaci Bemisia tabaci belongs to Semiptera Hemiptera Aleyrodidae Aleyrodidae, is a kind of world
The primary pest of property, causes huge economic loss to crop-planting, the production etc. of countries in the world.Bemisia tabaci passes through three aspects
Cause damages: (1) direct feeding plant juice causes plant growth bad；(2) cause coal dirty by way of secreting honeydew
Disease；(3) plant virus is propagated during feeding, causes breaking out for virosis.Loss caused by transmission via whitefly virus is most
To be serious, there is an urgent need to reinforce carrying out prevention and control to Bemisia tabaci and its virus of propagation.
Plant pest management relies primarily on chemical prevention in recent years, due to largely using chemical insecticide, so that very much
The Bemisia tabaci in area produces very high resistance to cause to break out greatly, also results in the outbreak of epidemic of the viroses of plant.Therefore,
There is an urgent need to seek the new way of small, the active strong prevention and treatment Bemisia tabaci of no pollution to the environment, dosage.
Plant volatile can influence insect to the selectivity of host, can attract or walk quickly and keep away pest.In recent years, " push-pull "
Strategy has received widespread attention as a kind of means of important pest control." pushing away " is walked quickly and kept away using the volatile matter of avoidance effect
Pest, and " drawing " attracts pest using the volatile matter of sucking action.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art, provide a kind of plant with sucking action
Volatile matter neophytadiene and its application, neophytadiene in test B Bemisia tabaci, Q Bemisia tabaci and take viruliferous B tobacco powder
Lice takes viruliferous Q Bemisia tabaci and has sucking action, can be used for preventing and treating Bemisia tabaci.
In order to solve the above-mentioned technical problems, the present invention provides a kind of Bemisia tabaci approach behavior adjusting control agent, the Bemisia tabacis
Approach behavior adjusting control agent is neophytadiene.
As a total technical concept, the present invention also provides a kind of insecticides, including pesticide and neophytadiene.
Above-mentioned insecticide, it is preferred that the concentration of neophytadiene is 4.8mg/L~19.2mg/L in the insecticide.
Above-mentioned insecticide, it is preferred that the pesticide is anabasine pesticide.Further, the anabasine pesticide
For Diacloden or imidacloprid.
As a total technical concept, the present invention also provides the answering in prevention and treatment Bemisia tabaci of the insecticide described in one kind
With.
Above-mentioned application, it is preferred that the application method are as follows: neophytadiene is added in anabasine pesticide and is uniformly mixed so as to obtain
Insecticide sprays application to insecticide on plant leaf blade.
As a total technical concept, the present invention also provides a kind of desinsection plates, including yellow plate and neophytadiene.
Above-mentioned desinsection plate, it is preferred that the concentration of the neophytadiene is 4.8~19.2mg/ yellow plate.
As a total technical concept, the present invention also provides the answering in prevention and treatment Bemisia tabaci of the desinsection plate described in one kind
With.
Above-mentioned application, it is preferred that the application method are as follows: neophytadiene sterling is sprayed on yellow plate, Jiang Huangban is suspended to
Bemisia tabaci is trapped and killed using the gluing of yellow plate in field.
Compared with the prior art, the advantages of the present invention are as follows:
(1) the present invention provides a kind of Bemisia tabaci approach behavior adjusting control agents, after applicant is to viral Bemisia tabaci feeding is carried
Tomato volatile matter studied, the tomato after carrying the Bemisia tabaci feeding of tomato yellow leaf curl virus, which has, attracts subsequent cigarette
The effect of aleyrodid is extracted volatile matter from tomato and is measured, and verified with standard items, finds one of terpene
Compound neophytadiene can attract Bemisia tabaci, it was demonstrated that attracting action of the neophytadiene to Bemisia tabaci.The present invention is first public
Neophytadiene is the component of candidate of potential attractant, can be used as adding ingredient and is added in yellow plate, or is added to insecticide
In be processed into sustained release agent, Bemisia tabaci is trapped and killed as Bemisia tabaci approach behavior adjusting control agent, for the prevention and treatment to Bemisia tabaci, tool
Have the advantages that safety non-pollution, dosage are small, active strong, there is extremely wide application prospect.
(2) the present invention provides a kind of insecticides, pesticide and neophytadiene are mixed, the addition of neophytadiene can be significant
Improve and the killing effect of Bemisia tabaci shown as to improve the quantity for killing Bemisia tabaci, and can extend killing Bemisia tabaci it is lasting when
Between.
(3) the present invention provides a kind of desinsection plate, neophytadiene is added on yellow plate, and Bemisia tabaci is attracted to jump on yellow plate, benefit
Bemisia tabaci is pasted and killed with the viscosity of yellow plate, the quantity for killing Bemisia tabaci can be significantly improved.
Detailed description of the invention
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, the technical scheme in the embodiment of the invention is clearly and completely described.
Fig. 1 is neophytadiene in the embodiment of the present invention 1 to the sucking action result figure of Bemisia tabaci.
Fig. 2 be in the embodiment of the present invention 1 after neophytadiene and other compounds to the sucking action result figure of Bemisia tabaci.
Specific embodiment
Below in conjunction with Figure of description and specific preferred embodiment, the invention will be further described, but not therefore and
It limits the scope of the invention.
Material employed in following embodiment and instrument are commercially available.
Embodiment 1:
A kind of Bemisia tabaci approach behavior adjusting control agent: neophytadiene.
Neophytadiene is investigated to the attracting action of Bemisia tabaci, investigates method are as follows:
(1) Bemisia tabaci is raised: taking four without worm cage, two of them are put into 4 healthy tomatoes, other two is put into
4 infection tomato yellow leaf curl virus tomato, take respectively 300 B, Q type Bemisia tabacis be put into four without in worm cage health and
It falls ill on tomato plant.After raising for 6 generations, four Bemisia tabacis: healthy B, health Q, band poison B, band poison Q are obtained, and after progress
Continuous test.
(2) volatile matter analysis method: headspace enrichment method is used, is carried out according to the following steps:
2.1, it the preparation of adsorption tube: is ready to clean the glass adsorption tube (diameter 3mm) of drying, takes a small amount of glass tampon
Enter at adsorption tube one third, weigh 0.1g Super Q adsorbent, pour into adsorption tube one end, then fills in a small amount of mineral wool (note
Anticipating between adsorbent and mineral wool will there are a little gaps).
2.2, the tomato of four population Bemisia tabaci feedings and control tomato are put into sealing collecting tank；Connected with sebific duct one end
Air compressor is connect, pot bottom is collected in one end connection sealing；At the top of another sebific duct one end connection sealing collecting tank, one end connects
Connect adsorption tube；Air compressor is opened, flow is adjusted to 1L/min, continuously collects 6h；Adsorption tube is extracted after 6h, is then used at once
The elution of 1600 μ L n-hexanes；By 0.22 μM of Millipore of membrane filtration of leacheate, by filtrate collection in the sample of 1.5mL
In product bottle, using the concentration of gas chromatographic measurement volatile matter.Processing and control all carry out 4 technologies repetitions and 3 secondary pollutants respectively
It learns and repeats.
2.3, GC conditions: injector temperature is 240 DEG C, and using temperature programming, initial column temperature is 50 DEG C, is kept
1min；240 DEG C are risen to 5 DEG C/min；240 DEG C, maintain 2min；300 DEG C are risen to 30 DEG C/min, 300 DEG C, maintains 5min.Matter
Spectral condition: ion source is the source electron bombardment EI, and excitation voltage 70eV, 150 DEG C of level four bars temperature, is swept by 300 DEG C of ion source temperature
Retouching range is 50m/z to 650m/z, scanning speed 0.5scans/s.Using areas of peak normalization method to the retention time of the compound
It is identified, and (No. CAS: 6789-80-6, purity 95.0%, purchase has from Beijing Wall Boke skill with standard items 3- hexenoic aldehyde
Limit responsible company), (No. CAS: 544-12-7, purity 98.0%, purchase is from the limited duty of Beijing Wall Boke skill for blatter alcohol
Ren company), (No. CAS: 3856-25-5, purity 98.0%, purchase is from Sigma-Aldrich), beta-myrcene (CAS for australene
Number: 123-35-3, purity 90.0%, purchase from Sigma-Aldrich), α-phellandrene (No. CAS: 99-83-2, purity
98.0%, purchase from Beijing Wall win science and technology limited Company), ρ-p-cymene (No. CAS: 99-87-6, purity 99.0%,
From Sigma-Aldrich), β-phellandrene, (No. CAS: 555-10-2, purchase is public from Beijing Wall Boke skill Limited Liability for purchase
Department), β-carypohyllene (No. CAS: 87-44-5, purity 98.0%, purchase from Sigma-Aldrich), α-humulene (No. CAS:
6753-98-6, purity 96.0%, purchase from Sigma-Aldrich), thujene (No. CAS: 2867-05-2, purity 96.0%,
Purchase from Beijing Wall win science and technology limited Company) and neophytadiene (No. CAS: 504-96-1, purity 95.0%, buy oneself
Win science and technology limited Company in Beijing Wall) it is compared one by one, the final concentration for determining volatile matter.
2.4, volatile matter is extracted and is identified:
According to volatile matter extraction and qualification result and 3- hexenoic aldehyde, blatter alcohol, australene, beta-myrcene, α-Chinese celery
Alkene, ρ-p-cymene, β-phellandrene, β-carypohyllene, α-humulene, thujene and neophytadiene are compared.According to volatile matter
The volatile content that measures of standard specimen, measurement result is referring to table 1.
Table 1: the concentration (unit: μ g24h of volatile matter after different population Bemisia tabaci feeding-1·g-1FW)
| Compound | Without Bemisia tabaci | Healthy B feeding | Healthy Q feeding | Band poison B feeding | Band poison Q feeding |
| 3- hexenoic aldehyde | 0.42 | 0.45 | 0.44 | 0.66 | 0.62 |
| Blatter alcohol | 0.13 | 0.44 | 0.49 | 0.42 | 0.45 |
| Australene | 0.03 | 0.06 | 0.07 | 0.07 | 0.07 |
| Beta-myrcene | 0.82 | 0.67 | 0.35 | 1.70 | 1.34 |
| α-phellandrene | 1.28 | 4.97 | 4.85 | 1.36 | 1.49 |
| ρ-p-cymene | 0.94 | 0.58 | 0.84 | 0.45 | 0.23 |
| β-phellandrene | 0.33 | 0.23 | 0.22 | 0.11 | 0.11 |
| β-carypohyllene | 1.93 | 1.23 | 1.22 | 1.37 | 1.37 |
| α-humulene | 0.07 | 0.16 | 0.25 | 0.13 | 0.14 |
| Thujene | - | - | - | 3.42 | 3.54 |
| Neophytadiene | - | - | - | - | 4.82 |
It is waved as it can be seen from table 1 plant can be changed compared with the plant of no Bemisia tabaci feeding, after Bemisia tabaci feeding plant
Send out object ingredient and concentration, wherein band after poison B feeding induction plant produce new volatile matter thujene, and with malicious Q feeding after
Induction plant produces new volatile matter thujene and neophytadiene.
(3) selectivity of the Bemisia tabaci to volatile matter: the specification of Y-piece is two brachium 18cm of upper end, the long 5cm of base arm, internal diameter
3cm, 75 degree of two-arm angle.Wherein an arm connects the glass tube for being placed with neophytadiene standard items to Y-piece；The empty glass of another arm connection
Glass pipe；Flow control is 100mL/min.The Bemisia tabaci for taking 100 four populations respectively, observes its selectivity；Each processing is seen
20min is examined, is repeated 10 times.Selective judgment criteria: it is greater than 5cm when Bemisia tabaci is stopped in any one arm of two-arm, and steadily
Continue 15s or more, then determines that it is made that selection to the corresponding plant of the arm.If Bemisia tabaci does not make a choice in 20min
It is invalid to be considered as, and carefully wipes Y-piece inside and outside wall with 95% ethyl alcohol immediately, and drying is stand-by.
Fig. 1 is neophytadiene in the embodiment of the present invention 1 to the sucking action result figure of Bemisia tabaci.It will be seen from figure 1 that Y-
In the test of type pipe, the Bemisia tabaci of four populations is intended to neophytadiene one end, and Bemisia tabaci quantity has aobvious compared with air one end
It writes difference (P < 0.05), it was demonstrated that neophytadiene has sucking action to Bemisia tabaci.
(4) influence of other compounds to neophytadiene: ρ-p-cymene and neophytadiene are mixed, according to step
(3) method investigates Bemisia tabaci to the selectivity of volatile matter, investigates result referring to fig. 2.
Figure it is seen that being carried out in neophytadiene and other compounds according to volatile matter ratio extracted in plant
After mixing, Bemisia tabaci still tends to one end of neophytadiene, and there are significance difference in Bemisia tabaci quantity and one end without neophytadiene
It is different.
It can be seen from the above, attracting action of the neophytadiene to Bemisia tabaci, can design Bemisia tabaci approach behavior adjusting control agent and be used for
The prevention and treatment of Bemisia tabaci has the advantages that safety non-pollution, dosage are small, active strong.
Embodiment 2:
A kind of insecticide of Bemisia tabaci, including neophytadiene and Diacloden.
Its application method are as follows: the neophytadiene of various concentration and Diacloden are mixed obtain insecticide respectively, by insecticide
It sprays application on plant leaf blade.Investigate before insecticide sprays and spray the quantity of rear Bemisia tabaci.The results are shown in Table 2 for investigation.
Insecticide 1:800mg/L Diacloden.
Insecticide 2:800mg/L Diacloden+4.8mg/L neophytadiene.
Insecticide 3:800mg/L Diacloden+9.6mg/L neophytadiene.
Insecticide 4:800mg/L Diacloden+14.4mg/L neophytadiene.
Insecticide 5:800mg/L Diacloden+19.2mg/L neophytadiene.
Table 2: Diacloden kills the comparison of Bemisia tabaci situation before and after increasing neophytadiene
From table 2 it can be seen that increasing the number of killing Bemisia tabaci than neophytadiene is not added after Diacloden increases neophytadiene
Amount, and extend the action time to Bemisia tabaci.
Embodiment 3:
A kind of desinsection plate of Bemisia tabaci, including neophytadiene and yellow plate.
The application method of its desinsection plate are as follows: the neophytadiene sterling of different content is sprayed on yellow plate, Jiang Huangban is suspended to field
Between, Bemisia tabaci is trapped and killed using the gluing of yellow plate.Investigate the quantity for the Bemisia tabaci that different desinsection plates attract.It investigates result and is listed in table 3
In.
Desinsection plate 1: yellow plate.
Desinsection plate 2: yellow plate+4.8mg neophytadiene.
Desinsection plate 2: yellow plate+9.6mg neophytadiene.
Desinsection plate 2: yellow plate+14.4mg neophytadiene.
Desinsection plate 2: yellow plate+19.2mg neophytadiene.
Table 3: yellow plate kills the comparison of Bemisia tabaci situation before and after increasing neophytadiene
| Processing | Initial Bemisia tabaci quantity on yellow plate | Bemisia tabaci quantity on yellow plate for 24 hours |
| Yellow plate | 0 | 98 |
| Yellow plate+4.8mg neophytadiene | 0 | 102 |
| Yellow plate+9.6mg neophytadiene | 0 | 113 |
| Yellow plate+14.4mg neophytadiene | 0 | 132 |
| Yellow plate+19.2mg neophytadiene | 0 | 106 |
As can be seen from Table 3, the trapping quantity to Bemisia tabaci can be significantly improved after yellow plate increase neophytadiene.
The above described is only a preferred embodiment of the present invention, being not intended to limit the present invention in any form.Though
So the present invention is disclosed as above with preferred embodiment, and however, it is not intended to limit the invention.It is any to be familiar with those skilled in the art
Member, in the case where not departing from Spirit Essence of the invention and technical solution, all using in the methods and techniques of the disclosure above
Appearance makes many possible changes and modifications or equivalent example modified to equivalent change to technical solution of the present invention.Therefore,
Anything that does not depart from the technical scheme of the invention are made to the above embodiment any simple according to the technical essence of the invention
Modification, equivalent replacement, equivalence changes and modification, all of which are still within the scope of protection of the technical scheme of the invention.
Claims (10)
1. a kind of Bemisia tabaci approach behavior adjusting control agent, which is characterized in that the Bemisia tabaci approach behavior adjusting control agent is neophytadiene.
2. a kind of insecticide, which is characterized in that including pesticide and neophytadiene.
3. insecticide according to claim 2, which is characterized in that the concentration of neophytadiene is 4.8mg/ in the insecticide
L~19.2mg/L.
4. insecticide according to claim 2, which is characterized in that the pesticide is anabasine pesticide.
5. application of the insecticide described in a kind of any one of claim 2 to 4 in prevention and treatment Bemisia tabaci.
6. application according to claim 5, which is characterized in that the application method are as follows: anabasine is added in neophytadiene
It is uniformly mixed so as to obtain insecticide in class pesticide, insecticide is sprayed application on plant leaf blade.
7. a kind of desinsection plate, which is characterized in that including yellow plate and neophytadiene.
8. desinsection plate according to claim 7, which is characterized in that the concentration of the neophytadiene are as follows: 4.8~19.2mg/
A Huang plate.
9. a kind of application of the desinsection plate described in claim 7 or 8 in prevention and treatment Bemisia tabaci.
10. application according to claim 9, which is characterized in that the application method are as follows: neophytadiene sterling is sprayed onto Huang
On plate, Jiang Huangban is suspended to field, traps and kills Bemisia tabaci using the gluing of yellow plate.
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| CN201910107424.0A CN109730083A (en) | 2019-02-02 | 2019-02-02 | Bemisia tabaci approach behavior adjusting control agent, the application of insecticide and insecticide, the application of desinsection plate and desinsection plate |
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| CN201910107424.0A CN109730083A (en) | 2019-02-02 | 2019-02-02 | Bemisia tabaci approach behavior adjusting control agent, the application of insecticide and insecticide, the application of desinsection plate and desinsection plate |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110835655A (en) * | 2019-12-10 | 2020-02-25 | 广东省农业科学院植物保护研究所 | Method for preventing and treating virus disease of trilobate cotton leaf curl |
| CN112042643A (en) * | 2020-09-09 | 2020-12-08 | 湖南省植物保护研究所 | Insect food attractant and preparation method and application thereof |
| CN114885942A (en) * | 2022-06-02 | 2022-08-12 | 青岛农业大学 | Attractant for bemisia tabaci and application of attractant |
-
2019
- 2019-02-02 CN CN201910107424.0A patent/CN109730083A/en active Pending
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| XIAOBIN SHI 等: "Plants Pre-Infested With Viruliferous MED/Q Cryptic Species Promotes Subsequent Bemisia tabaci Infestation", 《FRONTIERS IN MICROBIOLOGY》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110835655A (en) * | 2019-12-10 | 2020-02-25 | 广东省农业科学院植物保护研究所 | Method for preventing and treating virus disease of trilobate cotton leaf curl |
| CN112042643A (en) * | 2020-09-09 | 2020-12-08 | 湖南省植物保护研究所 | Insect food attractant and preparation method and application thereof |
| CN112042643B (en) * | 2020-09-09 | 2021-09-07 | 湖南省植物保护研究所 | Insect food attractant and preparation method and application thereof |
| CN114885942A (en) * | 2022-06-02 | 2022-08-12 | 青岛农业大学 | Attractant for bemisia tabaci and application of attractant |
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